Ube4b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube4b
Entrez Gene ID	63958
Full Name	ubiquitination factor E4B
Synonyms	4930551I19Rik,4933406G05Rik,AU014668,D4Bwg0973e,UFD2,UFD2a,Ufd2p,mKIAA0684
General protein information	Preferred Names ubiquitination factor E4B Names ubiquitin conjugation factor E4 B RING-type E3 ubiquitin transferase E4 B ubiquitin fusion degradation protein 2 ubiquitination factor E4B, UFD2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E2\|4 79.08 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006539088.4	XP_006539151.1	ubiquitin conjugation factor E4 B isoform X1
NM_022022.3	NP_071305.2	ubiquitin conjugation factor E4 B
XM_006539089.3	XP_006539152.1	ubiquitin conjugation factor E4 B isoform X2
XM_006539088.3	XP_006539151.1	ubiquitin conjugation factor E4 B isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F05269g	XP_455319.1
Eremothecium gossypii	AGOS_ADR096C	NP_984192.1
Neurospora crassa	NCU03357	XP_956466.1
Pan troglodytes (chimpanzee)	UBE4B	XP_001161561.1
Schizosaccharomyces pombe (fission yeast)	ufd2	NP_593630.1
Caenorhabditis elegans (roundworm)	ufd-2	NP_495691.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007870	XP_317622.4
Magnaporthe oryzae (rice blast fungus)	MGG_03255	XP_003716719.1
Rattus norvegicus (Norway rat)	Ube4b	NP_001258127.1
Drosophila melanogaster (fruit fly)	CG9934	NP_609597.1
Arabidopsis thaliana (thale cress)	AT5G15400	NP_568313.2
Mus musculus (house mouse)	Ube4b	NP_071305.2
Danio rerio (zebrafish)	ube4b	NP_919343.1
Xenopus tropicalis (tropical clawed frog)	ube4b	XP_004916084.1
Homo sapiens (human)	UBE4B	NP_001099032.1
Macaca mulatta (Rhesus monkey)	UBE4B	XP_001101836.1
Canis lupus familiaris (dog)	UBE4B	XP_005620583.1
Bos taurus (cattle)	UBE4B	NP_001095974.1
Gallus gallus (chicken)	UBE4B	XP_004947510.1
Saccharomyces cerevisiae (baker's yeast)	UFD2	NP_010091.2

Gene Ontology
Bibliography

Related articles in PubMed

Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis.
Starita LM, Pruneda JN, Lo RS, Fowler DM, Kim HJ, Hiatt JB, Shendure J, Brzovic PS, Fields S, Klevit RE
Proceedings of the National Academy of Sciences of the United States of America110(14)E1263-72(2013 Apr)

Islet α-, β-, and δ-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor.
Hunter CS, Dixit S, Cohen T, Ediger B, Wilcox C, Ferreira M, Westphal H, Stein R, May CL
Diabetes62(3)875-86(2013 Mar)

A novel conserved isoform of the ubiquitin ligase UFD2a/UBE4B is expressed exclusively in mature striated muscle cells.
Mammen AL, Mahoney JA, St Germain A, Badders N, Taylor JP, Rosen A, Spinette S
PloS one6(12)e28861(2011)

Increased E4 activity in mice leads to ubiquitin-containing aggregates and degeneration of hypothalamic neurons resulting in obesity.
Susaki E, Kaneko-Oshikawa C, Miyata K, Tabata M, Yamada T, Oike Y, Katagiri H, Nakayama KI
The Journal of biological chemistry285(20)15538-47(2010 May)

WldS can delay Wallerian degeneration in mice when interaction with valosin-containing protein is weakened.
Beirowski B, Morreale G, Conforti L, Mazzola F, Di Stefano M, Wilbrey A, Babetto E, Janeckova L, Magni G, Coleman MP
Neuroscience166(1)201-11(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34371	XM_006539088.4 Latest version!	Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu34372	XM_006539089.3 Latest version!	Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu12578	NM_022022.3 Latest version!	Mus musculus ubiquitination factor E4B (Ube4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$692.30 $989.00
View Old Accession Versions >>
OMu34371	XM_006539088.3	Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube4b ( XM_006539088.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539151.1 Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34371
Clone ID Related Accession (Same CDS sequence)	XM_006539088.4 , XM_006539088.3
Accession Version	XM_006539088.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT 
CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA 
GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC 
TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG 
GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG 
GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC 
AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC 
CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT 
GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG 
TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC 
CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC 
CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT 
GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA 
CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG 
AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA 
TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA 
CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG 
CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT 
GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG 
ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA 
GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG 
TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA 
CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA 
GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT 
TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC 
CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC 
AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG 
GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC 
GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC 
GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT 
CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC 
CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA 
GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA 
GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT 
GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT 
GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT 
GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC 
CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC 
CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG 
GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC 
CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT 
GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT 
TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT 
TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC 
TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT 
TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC 
AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC 
AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT 
TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC 
GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG 
TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG 
CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC 
CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA 
GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT 
AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG 
TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC 
TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT 
GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG 
TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA 
GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT 
CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC 
AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG 
CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539151.1
CDS	2257..6516
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCNMGSFSTSSLSSLYETSPAPSASLWSFVPALSPSFALPSHPAPQAAMPSQSLSPHGVAPQAAVPSQSLSPHGVAPQAAVPSQSLSPHGVAPQVAVPSQSLSTHGVAPQAAVPSQSLSTHGVAPQAAVPSQSLSTHGVAPQVAVPSQSLSTHGVAPQAAVPSPPLSPHSTASGNAAGSQPSSPRYRPYTVTHPWGSSPCPTPRSSILASSPGFPSHASSPRAVPASSSRQRPRSRVPAFPPASPSAASRRPSSLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLESDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAALVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNERLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRILDPAYPDVTLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTVMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH

Target ORF information:

RefSeq Version	XM_006539088.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539088.4

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT 
CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA 
GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC 
TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG 
GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG 
GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC 
AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC 
CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT 
GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG 
TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC 
CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC 
CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT 
GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA 
CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG 
AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA 
TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA 
CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG 
CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT 
GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG 
ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA 
GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG 
TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA 
CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA 
GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT 
TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC 
CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC 
AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG 
GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC 
GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC 
GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT 
CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC 
CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA 
GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA 
GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT 
GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT 
GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT 
GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC 
CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC 
CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG 
GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC 
CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT 
GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT 
TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT 
TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC 
TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT 
TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC 
AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC 
AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT 
TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC 
GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG 
TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG 
CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC 
CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA 
GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT 
AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG 
TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC 
TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT 
GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG 
TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA 
GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT 
CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC 
AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG 
CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube4b ( XM_006539088.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539151.1 Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34371
Clone ID Related Accession (Same CDS sequence)	XM_006539088.4 , XM_006539088.3
Accession Version	XM_006539088.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT 
CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA 
GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC 
TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG 
GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG 
GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC 
AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC 
CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT 
GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG 
TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC 
CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC 
CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT 
GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA 
CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG 
AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA 
TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA 
CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG 
CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT 
GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG 
ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA 
GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG 
TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA 
CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA 
GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT 
TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC 
CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC 
AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG 
GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC 
GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC 
GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT 
CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC 
CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA 
GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA 
GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT 
GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT 
GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT 
GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC 
CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC 
CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG 
GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC 
CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT 
GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT 
TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT 
TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC 
TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT 
TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC 
AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC 
AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT 
TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC 
GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG 
TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG 
CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC 
CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA 
GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT 
AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG 
TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC 
TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT 
GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG 
TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA 
GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT 
CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC 
AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG 
CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539151.1
CDS	725..4984
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCNMGSFSTSSLSSLYETSPAPSASLWSFVPALSPSFALPSHPAPQAAMPSQSLSPHGVAPQAAVPSQSLSPHGVAPQAAVPSQSLSPHGVAPQVAVPSQSLSTHGVAPQAAVPSQSLSTHGVAPQAAVPSQSLSTHGVAPQVAVPSQSLSTHGVAPQAAVPSPPLSPHSTASGNAAGSQPSSPRYRPYTVTHPWGSSPCPTPRSSILASSPGFPSHASSPRAVPASSSRQRPRSRVPAFPPASPSAASRRPSSLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLESDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAALVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNERLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRILDPAYPDVTLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTVMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH

Target ORF information:

RefSeq Version	XM_006539088.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539088.3

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT 
CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA 
GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC 
TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG 
GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG 
GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC 
AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC 
CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT 
GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG 
TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC 
CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC 
CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT 
GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA 
CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG 
AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA 
TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA 
CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG 
CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT 
GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG 
ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA 
GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG 
TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA 
CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA 
GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT 
TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC 
CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC 
AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG 
GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC 
GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC 
GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT 
CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC 
CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA 
GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA 
GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT 
GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT 
GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT 
GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC 
CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC 
CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG 
GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC 
CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT 
GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT 
TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT 
TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC 
TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT 
TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC 
AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC 
AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT 
TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC 
GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG 
TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG 
CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC 
CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA 
GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT 
AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG 
TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC 
TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT 
GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG 
TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA 
GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT 
CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC 
AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG 
CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube4b ( XM_006539089.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539152.1 Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA.

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CloneID	OMu34372
Clone ID Related Accession (Same CDS sequence)	XM_006539089.3
Accession Version	XM_006539089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ubiquitin conjugation factor E4 B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGAC GGAGGCGCCT GGCACGACTT GCTGGTGGAC AGACCTCCCA GCCGACCACC 
CCGCTTACAT CTCCCCAGAG GGAGAACCCT CCGGGACCTC CAATAGCTGC ATCAGCCCCA 
GGCCCCTCCC AGAGTCTTGG TCTCAATGTC CACAACATGA CCCCAGCTAC CTCCCCCATA 
GGTGCAGCAG GAGTTGCCCA TCGGAGCCAG AGCAGCGAAG GCGTCAGTTC TCTCAGCAGC 
TCACCTTCCA ACAGCCTTGA GACACAGTCT CAGTCCCTCT CCCGTTCCCA GAGCATGGAT 
ATTGACGGTG TCTCCTGTGA GAAAAGCATG TCCCAGGTGG ATGTGGATTC GGGAATTGAG 
AATATGGAGG TGGACGAGAA CGACCGAAGA GAAAAACGGA GCCTGAGTGA CAAGGAGCCT 
TCTTCAGGTC CTGAGGTGTC TGAAGAGCAA GCCTTACAGC TGGTCTGTAA GATCTTCCGT 
GTCTCCTGGA AGGACCGCGA CAGAGATGTC ATCTTCCTTT CTTCTCTCTC TGCGCAGTTT 
AAACAGAACC CAAAAGAAGT GTTCTCTGAT TTTAAGGATC TGATTGGCCA GATTTTAATG 
GAAGTACTGA TGATGTCAAC TCAGACACGA GATGAAAATC CATTTGCCAG TCTGACAGCC 
ACATCCCAGC CCATCGCCAC GGCAGCTCGG TCACCAGACC GCAATCTCAT GCTGAACACT 
GGCTCCAGTT CAGGAACAAG CCCCATGTTC TGCAACATGG GCTCCTTCAG CACCAGCTCG 
TTGTCTAGCC TCTATGAAAC GAGCCCCGCC CCCTCTGCCA GTTTATGGAG TTTTGTGCCA 
GCGCTCAGCC CATCCTTTGC CTTACCTTCC CACCCAGCCC CACAGGCGGC TATGCCTTCC 
CAGTCCCTTA GCCCCCATGG TGTAGCCCCA CAGGCGGCTG TGCCTTCCCA GTCCCTCAGC 
CCCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCAGT CCCTCAGCCC CCATGGTGTA 
GCCCCACAGG TGGCTGTGCC TTCCCAGTCC CTCAGCACCC ATGGTGTAGC CCCGCAGGCA 
GCTGTGCCTT CCCAGTCCCT CAGCACCCAT GGTGTAGCCC CGCAGGCGGC TGTGCCTTCC 
CAGTCCCTCA GCACCCATGG TGTAGCCCCG CAGGTGGCTG TGCCTTCCCA GTCCCTCAGC 
ACCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCCAC CCCTCAGCCC TCATAGTACA 
GCTTCTGGAA ATGCTGCCGG AAGCCAGCCC TCATCCCCGA GGTATCGGCC CTATACTGTC 
ACGCACCCTT GGGGGTCTTC ACCTTGCCCC ACCCCACGAT CCTCCATTCT GGCTAGCTCC 
CCAGGCTTCC CTTCCCACGC AAGTAGTCCC CGGGCTGTAC CTGCCAGCTC CTCCAGACAG 
AGGCCCCGCA GCAGGGTCCC CGCCTTCCCA CCTGCCTCGC CCAGTGCCGC CAGCAGACGT 
CCCTCCTCCC TGCGGATCTC TCCTAGTTTG GGAGCCTCTG GTGGAGCAAG TAACTGGGAT 
TCGTACAGTG ACCATTTCAC CATTGAAACC TGCAAAGAGA CGGACATGCT GAACTACCTC 
ATCGAGTGTT TTGACCGAGT TGGAATAGAG GAAAAAAAAG CACCAAAGAT GTGCAGCCAG 
CCAGCAGTCA GCCAGCTTCT GAGCAACATC CGCTCGCAGT GCATCTCCCA CACCGCACTA 
GTGCTACAAG GTTCCCTCAC ACAGCCCAGG TCCTTGCAAC AGCCATCCTT CCTCGTGCCG 
TACATGCTCT GCAGGAACCT CCCGTACGGC TTCATTCAAG AGCTGGTGAG GACCACTCAC 
CAGGATGAAG AGGTGTTCAA GCAGATCTTT ATCCCCATTT TACAAGGCCT GGCGCTGGCT 
GCCAAAGAGT GCTCCTTGGA AAGCGACTAC TTTAAGTACC CCCTCATGGC ACTGGGTGAA 
CTCTGTGAAA CCAAGTTTGG GAAGACACAC CCTATGTGCA ATTTGGTCGC TTCTTTACCC 
CTGTGGTTGC CGAAGTCCTT AAGCCCTGGT TCTGGGCGGG AGCTGCAGAG ACTCTCCTAC 
TTAGGGGCCT TCTTTAGCTT CTCAGTCTTC GCAGAGGACG ATGCAAAGGT GGTTGAGAAA 
TACTTCTCAG GGCCTGCCAT TACCCTGGAG AACACACGCG TGGTCAGCCA GTCACTACAG 
CATTACCTGG AGCTGGGACG GCAAGAACTT TTCAAGATTC TACATAGTAT TTTGTTAAAT 
GGCGAAACTC GTGAAGCGGC TCTCAGCTAC ATGGCGGCCC TTGTCAATGC CAACATGAAG 
AAAGCCCAGA TGCAGGCAGA TGATCGGTTG GTGTCTACAG ACGGGTTTAT GCTGAATCTC 
CTTTGGGTCC TGCAGCAGCT AAGTACAAAA ATCAAGTTAG AAACAGTTGA CCCCACTTAC 
ATATTCCACC CAAGATGTCG GATCACTCTC CCGAATGATG AGACACGGAT AAATGCAACG 
ATGGAGGATG TGAATGAGCG GCTGACAGAG CTCTATGGTG ATCAGCCTCC ATTTTCTGAG 
CCAAAATTCC CTACGGAATG TTTCTTTCTC ACCCTGCATG CTCATCACCT CTCTATTCTG 
CCTAGTTGTC GTCGCTACAT TCGCCGACTT CGAGCCATCC GCGAGCTCAA TAGAACCGTG 
GAAGATTTGA AAAATAATGA AAGCCAGTGG AAAGATTCCC CACTGGCCAC CAGACACCGT 
GAAATGCTGA AGCGCTGTAA AACTCAGCTT AAGAAACTGG TACGGTGCAA GGCCTGTGCT 
GACGCTGGCT TACTTGACGA GAGCTTCCTA AGAAGATGCC TGAATTTTTA TGGCCTTCTC 
ATTCAGCTGA TGCTGCGCAT CCTGGACCCT GCATATCCTG ACGTAACACT GCCTTTGAAT 
TCAGAAGTCC CCAAGGTATT TGCAGCATTG CCTGAGTTTT ATGTCGAAGA TGTTGCTGAA 
TTTTTATTTT TTATTGTACA ATACTCTCCT CAGGTGCTTT ATGAGCCCTG CACTCAGGAC 
ATTGTCATGT TCCTCGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA CCCTTACCTA 
GTGGCCAAGC TGGTGGAGGT CATGTTCATG ACCAACCCTT CTGTCCAGCC GAGAACTCAG 
AAGTTCTTTG AGATGATTGA GAACCATCCT CTCTCTACCA AACTGCTGGT GCCGTCCCTC 
ATGAAGTTCT ATACAGATGT CGAGCATACT GGAGCCACCA GCGAGTTCTA TGACAAGTTC 
ACAATTCGCT ATCACATTAG CACTATTTTT AAAAGCCTTT GGCAAAACAT AGCTCACCAT 
GGCACCTTCA TGGAGGAGTT CAATTCTGGA AAGCAGTTCG TTCGGTATAT CAATATGCTA 
ATAAATGACA CGACCTTTTT ACTGGACGAA AGTCTGGAGT CTCTGAAGCG GATCCATGAA 
GTGCAAGAAG AGATGAAGAA CAAAGAACAG TGGGACCAGC TGCCTCGGGA TCAGCAGCAG 
GCCCGGCAGT CTCAGCTTGC TCAGGACGAG CGTGTTTCCC GTTCTTATCT TGCCCTGGCA 
ACTGAAACCG TGGACATGTT CCATCTCCTC ACCAAGCAAG TCCAGAAGCC GTTCCTGAGG 
CCAGAGCTTG GTCCCCGATT AGCAGCCATG CTGAACTTTA ACCTGCAGCA GCTCTGCGGA 
CCCAAGTGCC GGGACCTGAA AGTTGAAAAC CCAGAGAAGT ATGGTTTTGA GCCAAAGAAG 
CTGTTGGACC AACTGACAGA TATTTATTTA CAATTGGACT GTGCTCGCTT TGCTAAAGCC 
ATCGCCGATG ACCAGCGATC CTACAGCAAG GAACTGTTTG AAGAAGTCAT TTCAAAGATG 
CGGAAGGCGG GAATCAAATC CACCATCGCA ATAGAGAAGT TTAAACTTCT TGCAGAGAAA 
GTGGAGGAAA TCGTGGCAAA GAATGCGCGG GCAGAAATAG ACTACAGCGA TGCCCCGGAC 
GAGTTCAGAG ACCCTCTGAT GGACACCCTG ATGACCGATC CCGTGAGACT GCCCTCTGGC 
ACCGTCATGG ACCGTTCTAT CATCCTGCGG CATCTGCTCA ACTCCCCCAC CGACCCCTTC 
AACCGCCAGA TGCTGACTGA GAGCATGCTG GAGCCAGTGC CAGAGCTAAA GGAGCAGATT 
CAGGCCTGGA TGAGAGAGAA ACAGAGCAGT GACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539152.1
CDS	209..4447
Translation	MIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCNMGSFSTSSLSSLYETSPAPSASLWSFVPALSPSFALPSHPAPQAAMPSQSLSPHGVAPQAAVPSQSLSPHGVAPQAAVPSQSLSPHGVAPQVAVPSQSLSTHGVAPQAAVPSQSLSTHGVAPQAAVPSQSLSTHGVAPQVAVPSQSLSTHGVAPQAAVPSPPLSPHSTASGNAAGSQPSSPRYRPYTVTHPWGSSPCPTPRSSILASSPGFPSHASSPRAVPASSSRQRPRSRVPAFPPASPSAASRRPSSLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLESDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAALVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNERLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRILDPAYPDVTLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTVMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH

Target ORF information:

RefSeq Version	XM_006539089.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539089.3

ORF Insert Sequence:

ATGATTCGAC GGAGGCGCCT GGCACGACTT GCTGGTGGAC AGACCTCCCA GCCGACCACC 
CCGCTTACAT CTCCCCAGAG GGAGAACCCT CCGGGACCTC CAATAGCTGC ATCAGCCCCA 
GGCCCCTCCC AGAGTCTTGG TCTCAATGTC CACAACATGA CCCCAGCTAC CTCCCCCATA 
GGTGCAGCAG GAGTTGCCCA TCGGAGCCAG AGCAGCGAAG GCGTCAGTTC TCTCAGCAGC 
TCACCTTCCA ACAGCCTTGA GACACAGTCT CAGTCCCTCT CCCGTTCCCA GAGCATGGAT 
ATTGACGGTG TCTCCTGTGA GAAAAGCATG TCCCAGGTGG ATGTGGATTC GGGAATTGAG 
AATATGGAGG TGGACGAGAA CGACCGAAGA GAAAAACGGA GCCTGAGTGA CAAGGAGCCT 
TCTTCAGGTC CTGAGGTGTC TGAAGAGCAA GCCTTACAGC TGGTCTGTAA GATCTTCCGT 
GTCTCCTGGA AGGACCGCGA CAGAGATGTC ATCTTCCTTT CTTCTCTCTC TGCGCAGTTT 
AAACAGAACC CAAAAGAAGT GTTCTCTGAT TTTAAGGATC TGATTGGCCA GATTTTAATG 
GAAGTACTGA TGATGTCAAC TCAGACACGA GATGAAAATC CATTTGCCAG TCTGACAGCC 
ACATCCCAGC CCATCGCCAC GGCAGCTCGG TCACCAGACC GCAATCTCAT GCTGAACACT 
GGCTCCAGTT CAGGAACAAG CCCCATGTTC TGCAACATGG GCTCCTTCAG CACCAGCTCG 
TTGTCTAGCC TCTATGAAAC GAGCCCCGCC CCCTCTGCCA GTTTATGGAG TTTTGTGCCA 
GCGCTCAGCC CATCCTTTGC CTTACCTTCC CACCCAGCCC CACAGGCGGC TATGCCTTCC 
CAGTCCCTTA GCCCCCATGG TGTAGCCCCA CAGGCGGCTG TGCCTTCCCA GTCCCTCAGC 
CCCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCAGT CCCTCAGCCC CCATGGTGTA 
GCCCCACAGG TGGCTGTGCC TTCCCAGTCC CTCAGCACCC ATGGTGTAGC CCCGCAGGCA 
GCTGTGCCTT CCCAGTCCCT CAGCACCCAT GGTGTAGCCC CGCAGGCGGC TGTGCCTTCC 
CAGTCCCTCA GCACCCATGG TGTAGCCCCG CAGGTGGCTG TGCCTTCCCA GTCCCTCAGC 
ACCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCCAC CCCTCAGCCC TCATAGTACA 
GCTTCTGGAA ATGCTGCCGG AAGCCAGCCC TCATCCCCGA GGTATCGGCC CTATACTGTC 
ACGCACCCTT GGGGGTCTTC ACCTTGCCCC ACCCCACGAT CCTCCATTCT GGCTAGCTCC 
CCAGGCTTCC CTTCCCACGC AAGTAGTCCC CGGGCTGTAC CTGCCAGCTC CTCCAGACAG 
AGGCCCCGCA GCAGGGTCCC CGCCTTCCCA CCTGCCTCGC CCAGTGCCGC CAGCAGACGT 
CCCTCCTCCC TGCGGATCTC TCCTAGTTTG GGAGCCTCTG GTGGAGCAAG TAACTGGGAT 
TCGTACAGTG ACCATTTCAC CATTGAAACC TGCAAAGAGA CGGACATGCT GAACTACCTC 
ATCGAGTGTT TTGACCGAGT TGGAATAGAG GAAAAAAAAG CACCAAAGAT GTGCAGCCAG 
CCAGCAGTCA GCCAGCTTCT GAGCAACATC CGCTCGCAGT GCATCTCCCA CACCGCACTA 
GTGCTACAAG GTTCCCTCAC ACAGCCCAGG TCCTTGCAAC AGCCATCCTT CCTCGTGCCG 
TACATGCTCT GCAGGAACCT CCCGTACGGC TTCATTCAAG AGCTGGTGAG GACCACTCAC 
CAGGATGAAG AGGTGTTCAA GCAGATCTTT ATCCCCATTT TACAAGGCCT GGCGCTGGCT 
GCCAAAGAGT GCTCCTTGGA AAGCGACTAC TTTAAGTACC CCCTCATGGC ACTGGGTGAA 
CTCTGTGAAA CCAAGTTTGG GAAGACACAC CCTATGTGCA ATTTGGTCGC TTCTTTACCC 
CTGTGGTTGC CGAAGTCCTT AAGCCCTGGT TCTGGGCGGG AGCTGCAGAG ACTCTCCTAC 
TTAGGGGCCT TCTTTAGCTT CTCAGTCTTC GCAGAGGACG ATGCAAAGGT GGTTGAGAAA 
TACTTCTCAG GGCCTGCCAT TACCCTGGAG AACACACGCG TGGTCAGCCA GTCACTACAG 
CATTACCTGG AGCTGGGACG GCAAGAACTT TTCAAGATTC TACATAGTAT TTTGTTAAAT 
GGCGAAACTC GTGAAGCGGC TCTCAGCTAC ATGGCGGCCC TTGTCAATGC CAACATGAAG 
AAAGCCCAGA TGCAGGCAGA TGATCGGTTG GTGTCTACAG ACGGGTTTAT GCTGAATCTC 
CTTTGGGTCC TGCAGCAGCT AAGTACAAAA ATCAAGTTAG AAACAGTTGA CCCCACTTAC 
ATATTCCACC CAAGATGTCG GATCACTCTC CCGAATGATG AGACACGGAT AAATGCAACG 
ATGGAGGATG TGAATGAGCG GCTGACAGAG CTCTATGGTG ATCAGCCTCC ATTTTCTGAG 
CCAAAATTCC CTACGGAATG TTTCTTTCTC ACCCTGCATG CTCATCACCT CTCTATTCTG 
CCTAGTTGTC GTCGCTACAT TCGCCGACTT CGAGCCATCC GCGAGCTCAA TAGAACCGTG 
GAAGATTTGA AAAATAATGA AAGCCAGTGG AAAGATTCCC CACTGGCCAC CAGACACCGT 
GAAATGCTGA AGCGCTGTAA AACTCAGCTT AAGAAACTGG TACGGTGCAA GGCCTGTGCT 
GACGCTGGCT TACTTGACGA GAGCTTCCTA AGAAGATGCC TGAATTTTTA TGGCCTTCTC 
ATTCAGCTGA TGCTGCGCAT CCTGGACCCT GCATATCCTG ACGTAACACT GCCTTTGAAT 
TCAGAAGTCC CCAAGGTATT TGCAGCATTG CCTGAGTTTT ATGTCGAAGA TGTTGCTGAA 
TTTTTATTTT TTATTGTACA ATACTCTCCT CAGGTGCTTT ATGAGCCCTG CACTCAGGAC 
ATTGTCATGT TCCTCGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA CCCTTACCTA 
GTGGCCAAGC TGGTGGAGGT CATGTTCATG ACCAACCCTT CTGTCCAGCC GAGAACTCAG 
AAGTTCTTTG AGATGATTGA GAACCATCCT CTCTCTACCA AACTGCTGGT GCCGTCCCTC 
ATGAAGTTCT ATACAGATGT CGAGCATACT GGAGCCACCA GCGAGTTCTA TGACAAGTTC 
ACAATTCGCT ATCACATTAG CACTATTTTT AAAAGCCTTT GGCAAAACAT AGCTCACCAT 
GGCACCTTCA TGGAGGAGTT CAATTCTGGA AAGCAGTTCG TTCGGTATAT CAATATGCTA 
ATAAATGACA CGACCTTTTT ACTGGACGAA AGTCTGGAGT CTCTGAAGCG GATCCATGAA 
GTGCAAGAAG AGATGAAGAA CAAAGAACAG TGGGACCAGC TGCCTCGGGA TCAGCAGCAG 
GCCCGGCAGT CTCAGCTTGC TCAGGACGAG CGTGTTTCCC GTTCTTATCT TGCCCTGGCA 
ACTGAAACCG TGGACATGTT CCATCTCCTC ACCAAGCAAG TCCAGAAGCC GTTCCTGAGG 
CCAGAGCTTG GTCCCCGATT AGCAGCCATG CTGAACTTTA ACCTGCAGCA GCTCTGCGGA 
CCCAAGTGCC GGGACCTGAA AGTTGAAAAC CCAGAGAAGT ATGGTTTTGA GCCAAAGAAG 
CTGTTGGACC AACTGACAGA TATTTATTTA CAATTGGACT GTGCTCGCTT TGCTAAAGCC 
ATCGCCGATG ACCAGCGATC CTACAGCAAG GAACTGTTTG AAGAAGTCAT TTCAAAGATG 
CGGAAGGCGG GAATCAAATC CACCATCGCA ATAGAGAAGT TTAAACTTCT TGCAGAGAAA 
GTGGAGGAAA TCGTGGCAAA GAATGCGCGG GCAGAAATAG ACTACAGCGA TGCCCCGGAC 
GAGTTCAGAG ACCCTCTGAT GGACACCCTG ATGACCGATC CCGTGAGACT GCCCTCTGGC 
ACCGTCATGG ACCGTTCTAT CATCCTGCGG CATCTGCTCA ACTCCCCCAC CGACCCCTTC 
AACCGCCAGA TGCTGACTGA GAGCATGCTG GAGCCAGTGC CAGAGCTAAA GGAGCAGATT 
CAGGCCTGGA TGAGAGAGAA ACAGAGCAGT GACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube4b ( NM_022022.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_071305.2 Mus musculus ubiquitination factor E4B (Ube4b), mRNA.

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CloneID	OMu12578
Clone ID Related Accession (Same CDS sequence)	NM_022022.3
Accession Version	NM_022022.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	ubiquitin conjugation factor E4 B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK016689.1, BY279585.1 and BC075620.1. On or before Apr 5, 2008 this sequence version replaced XM_001477015.1, NM_022022.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF260926.1, BC067402.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT 
CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA 
GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC 
TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG 
GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG 
GGCTCCTTCA GCACCAGCTC GTTGTCTAGT TTGGGAGCCT CTGGTGGAGC AAGTAACTGG 
GATTCGTACA GTGACCATTT CACCATTGAA ACCTGCAAAG AGACGGACAT GCTGAACTAC 
CTCATCGAGT GTTTTGACCG AGTTGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTGAGCAAC ATCCGCTCGC AGTGCATCTC CCACACCGCA 
CTAGTGCTAC AAGGTTCCCT CACACAGCCC AGGTCCTTGC AACAGCCATC CTTCCTCGTG 
CCGTACATGC TCTGCAGGAA CCTCCCGTAC GGCTTCATTC AAGAGCTGGT GAGGACCACT 
CACCAGGATG AAGAGGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCGCTG 
GCTGCCAAAG AGTGCTCCTT GGAAAGCGAC TACTTTAAGT ACCCCCTCAT GGCACTGGGT 
GAACTCTGTG AAACCAAGTT TGGGAAGACA CACCCTATGT GCAATTTGGT CGCTTCTTTA 
CCCCTGTGGT TGCCGAAGTC CTTAAGCCCT GGTTCTGGGC GGGAGCTGCA GAGACTCTCC 
TACTTAGGGG CCTTCTTTAG CTTCTCAGTC TTCGCAGAGG ACGATGCAAA GGTGGTTGAG 
AAATACTTCT CAGGGCCTGC CATTACCCTG GAGAACACAC GCGTGGTCAG CCAGTCACTA 
CAGCATTACC TGGAGCTGGG ACGGCAAGAA CTTTTCAAGA TTCTACATAG TATTTTGTTA 
AATGGCGAAA CTCGTGAAGC GGCTCTCAGC TACATGGCGG CCCTTGTCAA TGCCAACATG 
AAGAAAGCCC AGATGCAGGC AGATGATCGG TTGGTGTCTA CAGACGGGTT TATGCTGAAT 
CTCCTTTGGG TCCTGCAGCA GCTAAGTACA AAAATCAAGT TAGAAACAGT TGACCCCACT 
TACATATTCC ACCCAAGATG TCGGATCACT CTCCCGAATG ATGAGACACG GATAAATGCA 
ACGATGGAGG ATGTGAATGA GCGGCTGACA GAGCTCTATG GTGATCAGCC TCCATTTTCT 
GAGCCAAAAT TCCCTACGGA ATGTTTCTTT CTCACCCTGC ATGCTCATCA CCTCTCTATT 
CTGCCTAGTT GTCGTCGCTA CATTCGCCGA CTTCGAGCCA TCCGCGAGCT CAATAGAACC 
GTGGAAGATT TGAAAAATAA TGAAAGCCAG TGGAAAGATT CCCCACTGGC CACCAGACAC 
CGTGAAATGC TGAAGCGCTG TAAAACTCAG CTTAAGAAAC TGGTACGGTG CAAGGCCTGT 
GCTGACGCTG GCTTACTTGA CGAGAGCTTC CTAAGAAGAT GCCTGAATTT TTATGGCCTT 
CTCATTCAGC TGATGCTGCG CATCCTGGAC CCTGCATATC CTGACGTAAC ACTGCCTTTG 
AATTCAGAAG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTCGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TTTATGAGCC CTGCACTCAG 
GACATTGTCA TGTTCCTCGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAACCCTTAC 
CTAGTGGCCA AGCTGGTGGA GGTCATGTTC ATGACCAACC CTTCTGTCCA GCCGAGAACT 
CAGAAGTTCT TTGAGATGAT TGAGAACCAT CCTCTCTCTA CCAAACTGCT GGTGCCGTCC 
CTCATGAAGT TCTATACAGA TGTCGAGCAT ACTGGAGCCA CCAGCGAGTT CTATGACAAG 
TTCACAATTC GCTATCACAT TAGCACTATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCT GGAAAGCAGT TCGTTCGGTA TATCAATATG 
CTAATAAATG ACACGACCTT TTTACTGGAC GAAAGTCTGG AGTCTCTGAA GCGGATCCAT 
GAAGTGCAAG AAGAGATGAA GAACAAAGAA CAGTGGGACC AGCTGCCTCG GGATCAGCAG 
CAGGCCCGGC AGTCTCAGCT TGCTCAGGAC GAGCGTGTTT CCCGTTCTTA TCTTGCCCTG 
GCAACTGAAA CCGTGGACAT GTTCCATCTC CTCACCAAGC AAGTCCAGAA GCCGTTCCTG 
AGGCCAGAGC TTGGTCCCCG ATTAGCAGCC ATGCTGAACT TTAACCTGCA GCAGCTCTGC 
GGACCCAAGT GCCGGGACCT GAAAGTTGAA AACCCAGAGA AGTATGGTTT TGAGCCAAAG 
AAGCTGTTGG ACCAACTGAC AGATATTTAT TTACAATTGG ACTGTGCTCG CTTTGCTAAA 
GCCATCGCCG ATGACCAGCG ATCCTACAGC AAGGAACTGT TTGAAGAAGT CATTTCAAAG 
ATGCGGAAGG CGGGAATCAA ATCCACCATC GCAATAGAGA AGTTTAAACT TCTTGCAGAG 
AAAGTGGAGG AAATCGTGGC AAAGAATGCG CGGGCAGAAA TAGACTACAG CGATGCCCCG 
GACGAGTTCA GAGACCCTCT GATGGACACC CTGATGACCG ATCCCGTGAG ACTGCCCTCT 
GGCACCGTCA TGGACCGTTC TATCATCCTG CGGCATCTGC TCAACTCCCC CACCGACCCC 
TTCAACCGCC AGATGCTGAC TGAGAGCATG CTGGAGCCAG TGCCAGAGCT AAAGGAGCAG 
ATTCAGGCCT GGATGAGAGA GAAACAGAGC AGTGACCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071305.2
CDS	727..4248
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCNMGSFSTSSLSSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLESDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAALVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNERLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRILDPAYPDVTLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTVMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH

Target ORF information:

RefSeq Version	NM_022022.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitination factor E4B (Ube4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022022.3

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT 
CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA 
GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC 
TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG 
GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG 
AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG 
CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT 
TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT 
CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC 
CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG 
GGCTCCTTCA GCACCAGCTC GTTGTCTAGT TTGGGAGCCT CTGGTGGAGC AAGTAACTGG 
GATTCGTACA GTGACCATTT CACCATTGAA ACCTGCAAAG AGACGGACAT GCTGAACTAC 
CTCATCGAGT GTTTTGACCG AGTTGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTGAGCAAC ATCCGCTCGC AGTGCATCTC CCACACCGCA 
CTAGTGCTAC AAGGTTCCCT CACACAGCCC AGGTCCTTGC AACAGCCATC CTTCCTCGTG 
CCGTACATGC TCTGCAGGAA CCTCCCGTAC GGCTTCATTC AAGAGCTGGT GAGGACCACT 
CACCAGGATG AAGAGGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCGCTG 
GCTGCCAAAG AGTGCTCCTT GGAAAGCGAC TACTTTAAGT ACCCCCTCAT GGCACTGGGT 
GAACTCTGTG AAACCAAGTT TGGGAAGACA CACCCTATGT GCAATTTGGT CGCTTCTTTA 
CCCCTGTGGT TGCCGAAGTC CTTAAGCCCT GGTTCTGGGC GGGAGCTGCA GAGACTCTCC 
TACTTAGGGG CCTTCTTTAG CTTCTCAGTC TTCGCAGAGG ACGATGCAAA GGTGGTTGAG 
AAATACTTCT CAGGGCCTGC CATTACCCTG GAGAACACAC GCGTGGTCAG CCAGTCACTA 
CAGCATTACC TGGAGCTGGG ACGGCAAGAA CTTTTCAAGA TTCTACATAG TATTTTGTTA 
AATGGCGAAA CTCGTGAAGC GGCTCTCAGC TACATGGCGG CCCTTGTCAA TGCCAACATG 
AAGAAAGCCC AGATGCAGGC AGATGATCGG TTGGTGTCTA CAGACGGGTT TATGCTGAAT 
CTCCTTTGGG TCCTGCAGCA GCTAAGTACA AAAATCAAGT TAGAAACAGT TGACCCCACT 
TACATATTCC ACCCAAGATG TCGGATCACT CTCCCGAATG ATGAGACACG GATAAATGCA 
ACGATGGAGG ATGTGAATGA GCGGCTGACA GAGCTCTATG GTGATCAGCC TCCATTTTCT 
GAGCCAAAAT TCCCTACGGA ATGTTTCTTT CTCACCCTGC ATGCTCATCA CCTCTCTATT 
CTGCCTAGTT GTCGTCGCTA CATTCGCCGA CTTCGAGCCA TCCGCGAGCT CAATAGAACC 
GTGGAAGATT TGAAAAATAA TGAAAGCCAG TGGAAAGATT CCCCACTGGC CACCAGACAC 
CGTGAAATGC TGAAGCGCTG TAAAACTCAG CTTAAGAAAC TGGTACGGTG CAAGGCCTGT 
GCTGACGCTG GCTTACTTGA CGAGAGCTTC CTAAGAAGAT GCCTGAATTT TTATGGCCTT 
CTCATTCAGC TGATGCTGCG CATCCTGGAC CCTGCATATC CTGACGTAAC ACTGCCTTTG 
AATTCAGAAG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTCGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TTTATGAGCC CTGCACTCAG 
GACATTGTCA TGTTCCTCGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAACCCTTAC 
CTAGTGGCCA AGCTGGTGGA GGTCATGTTC ATGACCAACC CTTCTGTCCA GCCGAGAACT 
CAGAAGTTCT TTGAGATGAT TGAGAACCAT CCTCTCTCTA CCAAACTGCT GGTGCCGTCC 
CTCATGAAGT TCTATACAGA TGTCGAGCAT ACTGGAGCCA CCAGCGAGTT CTATGACAAG 
TTCACAATTC GCTATCACAT TAGCACTATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCT GGAAAGCAGT TCGTTCGGTA TATCAATATG 
CTAATAAATG ACACGACCTT TTTACTGGAC GAAAGTCTGG AGTCTCTGAA GCGGATCCAT 
GAAGTGCAAG AAGAGATGAA GAACAAAGAA CAGTGGGACC AGCTGCCTCG GGATCAGCAG 
CAGGCCCGGC AGTCTCAGCT TGCTCAGGAC GAGCGTGTTT CCCGTTCTTA TCTTGCCCTG 
GCAACTGAAA CCGTGGACAT GTTCCATCTC CTCACCAAGC AAGTCCAGAA GCCGTTCCTG 
AGGCCAGAGC TTGGTCCCCG ATTAGCAGCC ATGCTGAACT TTAACCTGCA GCAGCTCTGC 
GGACCCAAGT GCCGGGACCT GAAAGTTGAA AACCCAGAGA AGTATGGTTT TGAGCCAAAG 
AAGCTGTTGG ACCAACTGAC AGATATTTAT TTACAATTGG ACTGTGCTCG CTTTGCTAAA 
GCCATCGCCG ATGACCAGCG ATCCTACAGC AAGGAACTGT TTGAAGAAGT CATTTCAAAG 
ATGCGGAAGG CGGGAATCAA ATCCACCATC GCAATAGAGA AGTTTAAACT TCTTGCAGAG 
AAAGTGGAGG AAATCGTGGC AAAGAATGCG CGGGCAGAAA TAGACTACAG CGATGCCCCG 
GACGAGTTCA GAGACCCTCT GATGGACACC CTGATGACCG ATCCCGTGAG ACTGCCCTCT 
GGCACCGTCA TGGACCGTTC TATCATCCTG CGGCATCTGC TCAACTCCCC CACCGACCCC 
TTCAACCGCC AGATGCTGAC TGAGAGCATG CTGGAGCCAG TGCCAGAGCT AAAGGAGCAG 
ATTCAGGCCT GGATGAGAGA GAAACAGAGC AGTGACCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis.
Proceedings of the National Academy of Sciences of the United States of America110(14)E1263-72(2013 Apr)
Starita LM,Pruneda JN,Lo RS,Fowler DM,Kim HJ,Hiatt JB,Shendure J,Brzovic PS,Fields S,Klevit RE

Islet α-, β-, and δ-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor.
Diabetes62(3)875-86(2013 Mar)
Hunter CS,Dixit S,Cohen T,Ediger B,Wilcox C,Ferreira M,Westphal H,Stein R,May CL

A novel conserved isoform of the ubiquitin ligase UFD2a/UBE4B is expressed exclusively in mature striated muscle cells.
PloS one6(12)e28861(2011)
Mammen AL,Mahoney JA,St Germain A,Badders N,Taylor JP,Rosen A,Spinette S

Increased E4 activity in mice leads to ubiquitin-containing aggregates and degeneration of hypothalamic neurons resulting in obesity.
The Journal of biological chemistry285(20)15538-47(2010 May)
Susaki E,Kaneko-Oshikawa C,Miyata K,Tabata M,Yamada T,Oike Y,Katagiri H,Nakayama KI

WldS can delay Wallerian degeneration in mice when interaction with valosin-containing protein is weakened.
Neuroscience166(1)201-11(2010 Mar)
Beirowski B,Morreale G,Conforti L,Mazzola F,Di Stefano M,Wilbrey A,Babetto E,Janeckova L,Magni G,Coleman MP

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Ube4b cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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