Prkar1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkar1b
Entrez Gene ID	25521
Full Name	protein kinase cAMP-dependent type 1 regulatory subunit beta
Synonyms	RGD1563094,RIbeta
General protein information	Preferred Names protein kinase cAMP-dependent type 1 regulatory subunit beta Names cAMP-dependent protein kinase type I-beta regulatory subunit cAMP dependent protein kinase, subunit type 1 beta protein kinase, cAMP dependent regulatory, type I, beta protein kinase, cAMP-dependent, regulatory subunit type I beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q11
Summary	may play a role in protein amino acid phosphorylation and signal transduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008768942.2	XP_008767164.1	cAMP-dependent protein kinase type I-beta regulatory subunit isoform X1
NM_001033679.1	NP_001028851.2	cAMP-dependent protein kinase type I-beta regulatory subunit

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKAR1B	NP_001158231.1
Canis lupus familiaris (dog)	PRKAR1B	XP_005621278.1
Bos taurus (cattle)	PRKAR1B	NP_001068669.1
Mus musculus (house mouse)	Prkar1b	NP_001240819.1
Rattus norvegicus (Norway rat)	Prkar1b	NP_001028851.2
Gallus gallus (chicken)	PRKAR1B	XP_004945171.1
Danio rerio (zebrafish)	prkar1b	NP_001070091.1
Caenorhabditis elegans (roundworm)	kin-2	NP_001076771.1
Xenopus tropicalis (tropical clawed frog)	prkar1b	NP_001072922.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, Jahn O, Brose N, Herzog E
The EMBO journal33(2)157-70(2014 Jan)

The testis-specific Cα2 subunit of PKA is kinetically indistinguishable from the common Cα1 subunit of PKA.
Vetter MM, Zenn HM, Méndez E, van den Boom H, Herberg FW, Skålhegg BS
BMC biochemistry1240(2011 Aug)

An active protein kinase A (PKA) is involved in meiotic arrest of rat growing oocytes.
Kovo M, Kandli-Cohen M, Ben-Haim M, Galiani D, Carr DW, Dekel N
Reproduction (Cambridge, England)132(1)33-43(2006 Jul)

Localized effects of cAMP mediated by distinct routes of protein kinase A.
Taskén K, Aandahl EM
Physiological reviews84(1)137-67(2004 Jan)

Clustered distribution of cAMP-dependent protein kinase regulatory isoform RI alpha during the development of the rat brain.
Mucignat-Caretta C, Caretta A
The Journal of comparative neurology451(4)324-33(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prkar1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkar1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08543	NM_001033679.1 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type I regulatory subunit beta (Prkar1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa43131	XM_008768942.2 Latest version!	Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit beta (Prkar1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkar1b ( NM_001033679.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001028851.2 Rattus norvegicus protein kinase cAMP-dependent type I regulatory subunit beta (Prkar1b), mRNA.

Price & Availability↑

CloneID	ORa08543
Clone ID Related Accession (Same CDS sequence)	NM_001033679.1
Accession Version	NM_001033679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type I-beta regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104679.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC104679.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689604, SAMN02642708 [ECO:0000350] ##Evidence-Data-END##

ATGATGAGTC CACAGATCAT GTGTGTCTCA GTGTGTGGGG AAGGCATGGG ATGGGACCCT 
GTGGAAATAA GTCAGTCACA TGCTGAAGGC TGTGCTCAGA CTACAGGGCT GGAGGAAAAC 
AGGCAGATCC TGGCTCGGCA GAAGTCAAAC TCACAGTGTG ATTCCCACGA TGAGGAGATC 
TCCCCAACAC CTCCAAATCC TGTGGTAAAG GCACGTCGTC GACGGGGTGG CGTGAGTGCT 
GAAGTCTACA CTGAGGAAGA TGCCGTCTCC TACGTGAGGA AGGTCATTCC CAAGGACTAT 
AAGACCATGA CTGCGCTGGC CAAGGCCATT TCTAAGAACG TGCTCTTTTC TCACCTGGAT 
GACAACGAGA GAAGTGACAT ATTCGATGCC ATGTTTCCTG TCACCCACAT CGATGGGGAA 
ACAGTCATAC AGCAAGGGAA CGAAGGGGAT AATTTCTACG TGATTGACCA AGGAGAAGTA 
GACGTATATG TGAACGGGGA GTGGGTGACC AACATCAGTG AGGGGGGAAG CTTCGGGGAG 
CTGGCCCTCA TCTACGGCAC CCCTAGAGCG GCTACTGTGA AGGCCAAAAC GGACCTCAAG 
CTCTGGGGCA TCGACCGTGA CAGCTACAGG CGCATCCTCA TGGGAAGCAC ACTGAGGAAA 
CGCAAGATGT ATGAGGAATT CCTCAGCAAA GTCTCCATCT TAGAGTCTCT GGAGAAGTGG 
GAACGCCTCA CTGTAGCTGA TGCCCTGGAG CCTGTGCAGT TTGAAGATGG AGAGAAAATC 
GTTGTGCAGG GGGAGCCTGG GGATGATTTC TACATCATCA CAGAGGGTAC TGCTTCAGTC 
CTCCAGCGAC GATCCCCCAA CGAGGAGTAT GTGGAGGTGG GGCGCCTTGG ACCCTCTGAC 
TACTTTGGAG AGATTGCCCT GCTGCTGAAC CGGCCCCGTG CAGCCACTGT GGTGGCACGG 
GGTCCCCTCA AGTGTGTGAA GTTGGACCGG CCTCGTTTTG AGCGTGTCCT GGGCCCCTGC 
TCTGAGATCC TGAAGAGAAA CATCCAGCGT TACAACAGCT TCATCTCCCT AACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028851.2
CDS	65..1144
Translation	MMSPQIMCVSVCGEGMGWDPVEISQSHAEGCAQTTGLEENRQILARQKSNSQCDSHDEEISPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFSHLDDNERSDIFDAMFPVTHIDGETVIQQGNEGDNFYVIDQGEVDVYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKLDRPRFERVLGPCSEILKRNIQRYNSFISLTV

Target ORF information:

RefSeq Version	NM_001033679.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type I regulatory subunit beta (Prkar1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033679.1

ORF Insert Sequence:

ATGATGAGTC CACAGATCAT GTGTGTCTCA GTGTGTGGGG AAGGCATGGG ATGGGACCCT 
GTGGAAATAA GTCAGTCACA TGCTGAAGGC TGTGCTCAGA CTACAGGGCT GGAGGAAAAC 
AGGCAGATCC TGGCTCGGCA GAAGTCAAAC TCACAGTGTG ATTCCCACGA TGAGGAGATC 
TCCCCAACAC CTCCAAATCC TGTGGTAAAG GCACGTCGTC GACGGGGTGG CGTGAGTGCT 
GAAGTCTACA CTGAGGAAGA TGCCGTCTCC TACGTGAGGA AGGTCATTCC CAAGGACTAT 
AAGACCATGA CTGCGCTGGC CAAGGCCATT TCTAAGAACG TGCTCTTTTC TCACCTGGAT 
GACAACGAGA GAAGTGACAT ATTCGATGCC ATGTTTCCTG TCACCCACAT CGATGGGGAA 
ACAGTCATAC AGCAAGGGAA CGAAGGGGAT AATTTCTACG TGATTGACCA AGGAGAAGTA 
GACGTATATG TGAACGGGGA GTGGGTGACC AACATCAGTG AGGGGGGAAG CTTCGGGGAG 
CTGGCCCTCA TCTACGGCAC CCCTAGAGCG GCTACTGTGA AGGCCAAAAC GGACCTCAAG 
CTCTGGGGCA TCGACCGTGA CAGCTACAGG CGCATCCTCA TGGGAAGCAC ACTGAGGAAA 
CGCAAGATGT ATGAGGAATT CCTCAGCAAA GTCTCCATCT TAGAGTCTCT GGAGAAGTGG 
GAACGCCTCA CTGTAGCTGA TGCCCTGGAG CCTGTGCAGT TTGAAGATGG AGAGAAAATC 
GTTGTGCAGG GGGAGCCTGG GGATGATTTC TACATCATCA CAGAGGGTAC TGCTTCAGTC 
CTCCAGCGAC GATCCCCCAA CGAGGAGTAT GTGGAGGTGG GGCGCCTTGG ACCCTCTGAC 
TACTTTGGAG AGATTGCCCT GCTGCTGAAC CGGCCCCGTG CAGCCACTGT GGTGGCACGG 
GGTCCCCTCA AGTGTGTGAA GTTGGACCGG CCTCGTTTTG AGCGTGTCCT GGGCCCCTGC 
TCTGAGATCC TGAAGAGAAA CATCCAGCGT TACAACAGCT TCATCTCCCT AACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkar1b ( XM_008768942.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767164.1 Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit beta (Prkar1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43131
Clone ID Related Accession (Same CDS sequence)	XM_008768942.2
Accession Version	XM_008768942.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-dependent protein kinase type I-beta regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGATGAGTC CACAGATCAT GTGTGTCTCA GTGTGTGGGG AAGGCATGGG ATGGGACCCT 
GTGGAAATAA GTCAGTCACA TGCTGAAGGC TGTGCTCAGA CTACAGGGCT GGAGGAAAAC 
AGGCAGATCC TGGCTCGGCA GAAGTCAAAC TCACAGTGTG ATTCCCACGA TGAGGAGATC 
TCCCCAACAC CTCCAAATCC TGTGGTAAAG GCACGTCGTC GACGGGGTGG CGTGAGTGCT 
GAAGTCTACA CTGAGGAAGA TGCCGTCTCC TACGTGAGGA AGGTCATTCC CAAGGACTAT 
AAGACCATGA CTGCGCTGGC CAAGGCCATT TCTAAGAACG TGCTCTTTTC TCACCTGGAT 
GACAACGAGA GAAGTGACAT ATTCGATGCC ATGTTTCCTG TCACCCACAT CGATGGGGAA 
ACAGTCATAC AGCAAGGTAT GTTCCCAACT CCATCCGGAT GCTTGTCATC GGTCCTGACC 
CACTTAGGCC ACCTTAAGAA CTGCCTGAGG GGTTACCTCC AGGACAGGAG TATGTACGAC 
GTGGTCCAAG AACCTTGCAA AGCACCTGTC CTTTCCACTG GGGATGGTTG TGGGTTCCCC 
CACAGAGGAA GCGGCTATTA CAAGCCATGT GTCCCACTGA CCTTTCTCTC CTATTCTCCA 
AAGGTATATG TGAACGGGGA GTGGGTGACC AACATCAGTG AGGGGGGAAG CTTCGGGGAG 
CTGGCCCTCA TCTACGGCAC CCCTAGAGCG GCTACTGTGA AGGCCAAAAC GGACCTCAAG 
CTCTGGGGCA TCGACCGTGA CAGCTACAGG CGCATCCTCA TGGGAAGCAC ACTGAGGAAA 
CGCAAGATGT ATGAGGAATT CCTCAGCAAA GTCTCCATCT TAGAGTCTCT GGAGAAGTGG 
GAACGCCTCA CTGTAGCTGA TGCCCTGGAG CCTGTGCAGT TTGAAGATGG AGAGAAAATC 
GTTGTGCAGG GGGAGCCTGG GGATGATTTC TACATCATCA CAGAGGGTAC TGCTTCAGTC 
CTCCAGCGAC GATCCCCCAA CGAGGAGTAT GTGGAGGTGG GGCGCCTTGG ACCCTCTGAC 
TACTTTGGAG AGATTGCCCT GCTGCTGAAC CGGCCCCGTG CAGCCACTGT GGTGGCACGG 
GGTCCCCTCA AGTGTGTGAA GTTGGACCGG CCTCGTTTTG AGCGTGTCCT GGGCCCCTGC 
TCTGAGATCC TGAAGAGAAA CATCCAGCGT TACAACAGCT TCATCTCCCT AACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767164.1
CDS	94..1353
Translation	MMSPQIMCVSVCGEGMGWDPVEISQSHAEGCAQTTGLEENRQILARQKSNSQCDSHDEEISPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFSHLDDNERSDIFDAMFPVTHIDGETVIQQGMFPTPSGCLSSVLTHLGHLKNCLRGYLQDRSMYDVVQEPCKAPVLSTGDGCGFPHRGSGYYKPCVPLTFLSYSPKVYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKLDRPRFERVLGPCSEILKRNIQRYNSFISLTV

Target ORF information:

RefSeq Version	XM_008768942.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cAMP-dependent type 1 regulatory subunit beta (Prkar1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768942.2

ORF Insert Sequence:

ATGATGAGTC CACAGATCAT GTGTGTCTCA GTGTGTGGGG AAGGCATGGG ATGGGACCCT 
GTGGAAATAA GTCAGTCACA TGCTGAAGGC TGTGCTCAGA CTACAGGGCT GGAGGAAAAC 
AGGCAGATCC TGGCTCGGCA GAAGTCAAAC TCACAGTGTG ATTCCCACGA TGAGGAGATC 
TCCCCAACAC CTCCAAATCC TGTGGTAAAG GCACGTCGTC GACGGGGTGG CGTGAGTGCT 
GAAGTCTACA CTGAGGAAGA TGCCGTCTCC TACGTGAGGA AGGTCATTCC CAAGGACTAT 
AAGACCATGA CTGCGCTGGC CAAGGCCATT TCTAAGAACG TGCTCTTTTC TCACCTGGAT 
GACAACGAGA GAAGTGACAT ATTCGATGCC ATGTTTCCTG TCACCCACAT CGATGGGGAA 
ACAGTCATAC AGCAAGGTAT GTTCCCAACT CCATCCGGAT GCTTGTCATC GGTCCTGACC 
CACTTAGGCC ACCTTAAGAA CTGCCTGAGG GGTTACCTCC AGGACAGGAG TATGTACGAC 
GTGGTCCAAG AACCTTGCAA AGCACCTGTC CTTTCCACTG GGGATGGTTG TGGGTTCCCC 
CACAGAGGAA GCGGCTATTA CAAGCCATGT GTCCCACTGA CCTTTCTCTC CTATTCTCCA 
AAGGTATATG TGAACGGGGA GTGGGTGACC AACATCAGTG AGGGGGGAAG CTTCGGGGAG 
CTGGCCCTCA TCTACGGCAC CCCTAGAGCG GCTACTGTGA AGGCCAAAAC GGACCTCAAG 
CTCTGGGGCA TCGACCGTGA CAGCTACAGG CGCATCCTCA TGGGAAGCAC ACTGAGGAAA 
CGCAAGATGT ATGAGGAATT CCTCAGCAAA GTCTCCATCT TAGAGTCTCT GGAGAAGTGG 
GAACGCCTCA CTGTAGCTGA TGCCCTGGAG CCTGTGCAGT TTGAAGATGG AGAGAAAATC 
GTTGTGCAGG GGGAGCCTGG GGATGATTTC TACATCATCA CAGAGGGTAC TGCTTCAGTC 
CTCCAGCGAC GATCCCCCAA CGAGGAGTAT GTGGAGGTGG GGCGCCTTGG ACCCTCTGAC 
TACTTTGGAG AGATTGCCCT GCTGCTGAAC CGGCCCCGTG CAGCCACTGT GGTGGCACGG 
GGTCCCCTCA AGTGTGTGAA GTTGGACCGG CCTCGTTTTG AGCGTGTCCT GGGCCCCTGC 
TCTGAGATCC TGAAGAGAAA CATCCAGCGT TACAACAGCT TCATCTCCCT AACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
The EMBO journal33(2)157-70(2014 Jan)
Biesemann C,Grønborg M,Luquet E,Wichert SP,Bernard V,Bungers SR,Cooper B,Varoqueaux F,Li L,Byrne JA,Urlaub H,Jahn O,Brose N,Herzog E

The testis-specific Cα2 subunit of PKA is kinetically indistinguishable from the common Cα1 subunit of PKA.
BMC biochemistry1240(2011 Aug)
Vetter MM,Zenn HM,Méndez E,van den Boom H,Herberg FW,Skålhegg BS

An active protein kinase A (PKA) is involved in meiotic arrest of rat growing oocytes.
Reproduction (Cambridge, England)132(1)33-43(2006 Jul)
Kovo M,Kandli-Cohen M,Ben-Haim M,Galiani D,Carr DW,Dekel N

Localized effects of cAMP mediated by distinct routes of protein kinase A.
Physiological reviews84(1)137-67(2004 Jan)
Taskén K,Aandahl EM

Clustered distribution of cAMP-dependent protein kinase regulatory isoform RI alpha during the development of the rat brain.
The Journal of comparative neurology451(4)324-33(2002 Sep)
Mucignat-Caretta C,Caretta A

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Prkar1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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