Tnf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnf
Entrez Gene ID	24835
Full Name	tumor necrosis factor
Synonyms	RATTNF,TNF-alpha,Tnfa
General protein information	Preferred Names tumor necrosis factor Names tumor necrosis factor cachectin tumor necrosis factor (TNF superfamily, member 2) tumor necrosis factor alpha tumor necrosis factor ligand superfamily member 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012675.3	NP_036807.1	tumor necrosis factor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnf	NP_038721.1
Canis lupus familiaris (dog)	TNF	NP_001003244.4
Bos taurus (cattle)	TNF	NP_776391.2
Rattus norvegicus (Norway rat)	Tnf	NP_036807.1
Macaca mulatta (Rhesus monkey)	TNF	NP_001040614.1
Xenopus tropicalis (tropical clawed frog)	tnf	NP_001107143.1
Homo sapiens (human)	TNF	NP_000585.2
Pan troglodytes (chimpanzee)	TNF	NP_001038976.1

Gene Ontology
Bibliography

Related articles in PubMed

Ellagic Acid Prevents Oxidative Stress, Inflammation, and Histopathological Alterations in Acrylamide-Induced Hepatotoxicity in Wistar Rats.
Karimi MY, Fatemi I, Kalantari H, Mombeini MA, Mehrzadi S, Goudarzi M
Journal of dietary supplements1-12(2019 Jul)

Sitagliptin ameliorates thioacetamide-induced acute liver injury via modulating TLR4/NF-KB signaling pathway in mice.
El-Kashef DH, Serrya MS
Life sciences228266-273(2019 Jul)

Fibronectin inhibitor pUR4 attenuates tumor necrosis factor α-induced endothelial hyperpermeability by modulating β1 integrin activation.
Lee TH, Hsieh ST, Chiang HY
Journal of biomedical science26(1)37(2019 May)

MicroRNA-181c Inhibits Interleukin-6-mediated Beta Cell Apoptosis by Targeting TNF-α Expression.
Oh YS, Bae GD, Park EY, Jun HS
Molecules (Basel, Switzerland)24(7)(2019 Apr)

Ar Rochmah M, Harini IM, Septyaningtrias DE, Sari DCR, Susilowati R
BioMed research international20192649281(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tnf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13562	NM_012675.3 Latest version!	Rattus norvegicus tumor necrosis factor (Tnf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnf ( NM_012675.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036807.1 Rattus norvegicus tumor necrosis factor (Tnf), mRNA.

Price & Availability↑

CloneID	ORa13562
Clone ID Related Accession (Same CDS sequence)	NM_012675.3
Accession Version	NM_012675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Rattus norvegicus(Norway rat)
Product	tumor necrosis factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX883046.1. On Oct 1, 2009 this sequence version replaced NM_012675.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107671.1, HQ201305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACGG AAAGCATGAT CCGAGATGTG GAACTGGCAG AGGAGGCGCT CCCCAAAAAG 
ATGGGGGGCC TCCAGAACTC CAGGCGGTGT CTGTGCCTCA GCCTCTTCTC ATTCCTGCTC 
GTGGCGGGGG CCACCACGCT CTTCTGTCTA CTGAACTTCG GGGTGATCGG TCCCAACAAG 
GAGGAGAAGT TCCCAAATGG GCTCCCTCTC ATCAGTTCCA TGGCCCAGAC CCTCACACTC 
AGATCATCTT CTCAAAACTC GAGTGACAAG CCCGTAGCCC ACGTCGTAGC AAACCACCAA 
GCGGAGGAGC AGCTGGAGTG GCTGAGCCAG CGTGCCAACG CCCTCCTGGC CAATGGCATG 
GATCTCAAAG ACAACCAACT GGTGGTACCA GCAGATGGGC TGTACCTTAT CTACTCCCAG 
GTTCTCTTCA AGGGACAAGG CTGCCCCGAC TACGTGCTCC TCACCCACAC CGTCAGCCGA 
TTTGCCATTT CATACCAGGA GAAAGTCAGC CTCCTCTCTG CCATCAAGAG CCCTTGCCCT 
AAGGACACCC CTGAGGGAGC TGAGCTCAAG CCCTGGTATG AGCCCATGTA CCTGGGAGGA 
GTCTTCCAGC TGGAGAAGGG GGACCTGCTC AGCGCTGAGG TCAACCTGCC CAAGTACTTA 
GACATCACGG AGTCCGGGCA GGTCTACTTT GGAGTCATTG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036807.1
CDS	145..852
Translation	MSTESMIRDVELAEEALPKKMGGLQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPNKEEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL

Target ORF information:

RefSeq Version	NM_012675.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tumor necrosis factor (Tnf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012675.3

ORF Insert Sequence:

ATGAGCACGG AAAGCATGAT CCGAGATGTG GAACTGGCAG AGGAGGCGCT CCCCAAAAAG 
ATGGGGGGCC TCCAGAACTC CAGGCGGTGT CTGTGCCTCA GCCTCTTCTC ATTCCTGCTC 
GTGGCGGGGG CCACCACGCT CTTCTGTCTA CTGAACTTCG GGGTGATCGG TCCCAACAAG 
GAGGAGAAGT TCCCAAATGG GCTCCCTCTC ATCAGTTCCA TGGCCCAGAC CCTCACACTC 
AGATCATCTT CTCAAAACTC GAGTGACAAG CCCGTAGCCC ACGTCGTAGC AAACCACCAA 
GCGGAGGAGC AGCTGGAGTG GCTGAGCCAG CGTGCCAACG CCCTCCTGGC CAATGGCATG 
GATCTCAAAG ACAACCAACT GGTGGTACCA GCAGATGGGC TGTACCTTAT CTACTCCCAG 
GTTCTCTTCA AGGGACAAGG CTGCCCCGAC TACGTGCTCC TCACCCACAC CGTCAGCCGA 
TTTGCCATTT CATACCAGGA GAAAGTCAGC CTCCTCTCTG CCATCAAGAG CCCTTGCCCT 
AAGGACACCC CTGAGGGAGC TGAGCTCAAG CCCTGGTATG AGCCCATGTA CCTGGGAGGA 
GTCTTCCAGC TGGAGAAGGG GGACCTGCTC AGCGCTGAGG TCAACCTGCC CAAGTACTTA 
GACATCACGG AGTCCGGGCA GGTCTACTTT GGAGTCATTG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ellagic Acid Prevents Oxidative Stress, Inflammation, and Histopathological Alterations in Acrylamide-Induced Hepatotoxicity in Wistar Rats.
Journal of dietary supplements1-12(2019 Jul)
Karimi MY,Fatemi I,Kalantari H,Mombeini MA,Mehrzadi S,Goudarzi M

Sitagliptin ameliorates thioacetamide-induced acute liver injury via modulating TLR4/NF-KB signaling pathway in mice.
Life sciences228266-273(2019 Jul)
El-Kashef DH,Serrya MS

Fibronectin inhibitor pUR4 attenuates tumor necrosis factor α-induced endothelial hyperpermeability by modulating β1 integrin activation.
Journal of biomedical science26(1)37(2019 May)
Lee TH,Hsieh ST,Chiang HY

MicroRNA-181c Inhibits Interleukin-6-mediated Beta Cell Apoptosis by Targeting TNF-α Expression.
Molecules (Basel, Switzerland)24(7)(2019 Apr)
Oh YS,Bae GD,Park EY,Jun HS

BioMed research international20192649281(2019)
Ar Rochmah M,Harini IM,Septyaningtrias DE,Sari DCR,Susilowati R