TNF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	7124
Full Name	tumor necrosis factor
Synonyms	DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F
General protein information	Preferred Names tumor necrosis factor Names tumor necrosis factor APC1 protein TNF, macrophage-derived TNF, monocyte-derived TNF-a cachectin tumor necrosis factor ligand 1F tumor necrosis factor ligand superfamily member 2 tumor necrosis factor-alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008].
Disorder MIM:	191160
Disorder Html:	{Malaria, cerebral, susceptibility to}, 611162 (3); {Septic shock, susceptibility to} (3); {Asthma, susceptibility to}, 600807 (3); {Dementia, vascular, susceptibility to} (3); {Migraine without aura, susceptibility to}, 157300 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000594.3	NP_000585.2	tumor necrosis factor
NM_000594.4	NP_000585.2	tumor necrosis factor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnf	NP_038721.1
Canis lupus familiaris (dog)	TNF	NP_001003244.4
Bos taurus (cattle)	TNF	NP_776391.2
Rattus norvegicus (Norway rat)	Tnf	NP_036807.1
Macaca mulatta (Rhesus monkey)	TNF	NP_001040614.1
Xenopus tropicalis (tropical clawed frog)	tnf	NP_001107143.1
Homo sapiens (human)	TNF	NP_000585.2
Pan troglodytes (chimpanzee)	TNF	NP_001038976.1

Gene Ontology
Bibliography

Related articles in PubMed

TNFα Induces Multidrug Resistance-Associated Protein 4 Expression through p38-E2F1-Nrf2 Signaling in Obstructive Cholestasis.
Lian W, Liu X, Chen W
Yonsei medical journal60(11)1045-1053(2019 Nov)

Polymorphism of the IL-1β, TNF, IL-1RA and IL-4 Cytokine Genes Significantly Increases the Risk of Preterm Birth.
Belousova VS, Svitich OA, Timokhina EV, Strizhakov AN, Bogomazova IM
Biochemistry. Biokhimiia84(9)1040-1046(2019 Sep)

Oxidative stress and biomarker of TNF-α, MDA and FRAP in hypertension.
Verma MK, Jaiswal A, Sharma P, Kumar P, Singh AN
Journal of medicine and life12(3)253-259(2019 Jul-Sep)

TLR4/NF-κB signaling pathway gene single nucleotide polymorphisms alter gene expression levels and affect ARDS occurrence and prognosis outcomes.
Ding Y, Feng Q, Chen J, Song J
Medicine98(26)e16029(2019 Jun)

Quantification of TNF-
Martínez-Aguilar VM, Carrillo-Ávila BA, Sauri-Esquivel EA, Guzmán-Marín E, Jiménez-Coello M, Escobar-García DM, Pozos-Guillén A
BioMed research international20197984891(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27508	NM_000594.4 Latest version!	Homo sapiens tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu27508	NM_000594.3	Homo sapiens tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( NM_000594.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000585.2 Homo sapiens tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OHu27508
Clone ID Related Accession (Same CDS sequence)	NM_000594.3 , NM_000594.4
Accession Version	NM_000594.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514649600000
Organism	Homo sapiens(human)
Product	tumor necrosis factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP215875.1, BC028148.1 and CA306559.1. This sequence is a reference standard in the RefSeqGene project. On Jul 12, 2012 this sequence version replaced NM_000594.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.559704.1, BC028148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC 
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG 
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT 
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG 
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA 
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC 
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG 
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC 
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000585.2
CDS	176..877
Translation	MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_000594.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000594.3

ORF Insert Sequence:

ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC 
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG 
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT 
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG 
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA 
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC 
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG 
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC 
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( NM_000594.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000585.2 Homo sapiens tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OHu27508
Clone ID Related Accession (Same CDS sequence)	NM_000594.3 , NM_000594.4
Accession Version	NM_000594.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	tumor necrosis factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL662801.7, BP215875.1, BC028148.1 and CA306559.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000594.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028148.1, X01394.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000449264.3/ ENSP00000398698.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC 
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG 
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT 
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG 
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA 
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC 
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG 
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC 
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000585.2
CDS	178..879
Translation	MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_000594.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000594.4

ORF Insert Sequence:

ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC 
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG 
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT 
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG 
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA 
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC 
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG 
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC 
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TNFα Induces Multidrug Resistance-Associated Protein 4 Expression through p38-E2F1-Nrf2 Signaling in Obstructive Cholestasis.
Yonsei medical journal60(11)1045-1053(2019 Nov)
Lian W,Liu X,Chen W

Polymorphism of the IL-1β, TNF, IL-1RA and IL-4 Cytokine Genes Significantly Increases the Risk of Preterm Birth.
Biochemistry. Biokhimiia84(9)1040-1046(2019 Sep)
Belousova VS,Svitich OA,Timokhina EV,Strizhakov AN,Bogomazova IM

Oxidative stress and biomarker of TNF-α, MDA and FRAP in hypertension.
Journal of medicine and life12(3)253-259(2019 Jul-Sep)
Verma MK,Jaiswal A,Sharma P,Kumar P,Singh AN

TLR4/NF-κB signaling pathway gene single nucleotide polymorphisms alter gene expression levels and affect ARDS occurrence and prognosis outcomes.
Medicine98(26)e16029(2019 Jun)
Ding Y,Feng Q,Chen J,Song J

Quantification of TNF-
BioMed research international20197984891(2019)
Martínez-Aguilar VM,Carrillo-Ávila BA,Sauri-Esquivel EA,Guzmán-Marín E,Jiménez-Coello M,Escobar-García DM,Pozos-Guillén A

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TNF cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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