MAN2A2 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_009429842.2	XP_009428117.1	alpha-mannosidase 2x isoform X1
XM_024349368.1	XP_024205136.1	alpha-mannosidase 2x isoform X2
XM_016927436.2	XP_016782925.1	alpha-mannosidase 2x isoform X1
XM_009429844.3	XP_009428119.1	alpha-mannosidase 2x isoform X1
XM_016927436.1	XP_016782925.1	alpha-mannosidase 2x isoform X1
XM_016927438.1	XP_016782927.1	alpha-mannosidase 2x isoform X2
XM_009429844.2	XP_009428119.1	alpha-mannosidase 2x isoform X1
XM_016927437.2	XP_016782926.1	alpha-mannosidase 2x isoform X1
XM_009429842.3	XP_009428117.1	alpha-mannosidase 2x isoform X1
XM_016927437.1	XP_016782926.1	alpha-mannosidase 2x isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc19091	XM_024349368.1 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPc50248	XM_016927437.2 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc50248	XM_016927436.2 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc50248	XM_009429842.3 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc50248	XM_009429844.3 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc69099	XM_016927437.1 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc69099	XM_016927436.1 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc69099	XM_009429842.2 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc69099	XM_009429844.2 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc69100	XM_016927438.1 Latest version!	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

MAN2A2 ( XM_024349368.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024205136.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc19091
Clone ID Related Accession (Same CDS sequence)	XM_024349368.1
Accession Version	XM_024349368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA 
GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ACGCAGCTCC CAGGCCCTGG TCTGCGCTCA 
TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACCCTC 
CAGGCTGAGG AGGACACCCT GCCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG 
GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG 
ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG 
GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024205136.1
CDS	391..3843
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_024349368.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024349368.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA 
GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ACGCAGCTCC CAGGCCCTGG TCTGCGCTCA 
TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACCCTC 
CAGGCTGAGG AGGACACCCT GCCCTCGGCG GAGACCGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG 
GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG 
ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG 
GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_016927436.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016782925.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc50248
Clone ID Related Accession (Same CDS sequence)	XM_016927436.2 , XM_009429844.3 , XM_016927437.2 , XM_009429842.3
Accession Version	XM_016927436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016782925.1
CDS	364..3891
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_016927436.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927436.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_009429844.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009428119.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

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CloneID	OPc50248
Clone ID Related Accession (Same CDS sequence)	XM_016927436.2 , XM_009429844.3 , XM_016927437.2 , XM_009429842.3
Accession Version	XM_009429844.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009429844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009428119.1
CDS	289..3816
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_009429844.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009429844.3

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_016927437.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016782926.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

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CloneID	OPc50248
Clone ID Related Accession (Same CDS sequence)	XM_016927436.2 , XM_009429844.3 , XM_016927437.2 , XM_009429842.3
Accession Version	XM_016927437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016927437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016782926.1
CDS	223..3750
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_016927437.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927437.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_009429842.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009428117.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

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CloneID	OPc50248
Clone ID Related Accession (Same CDS sequence)	XM_016927436.2 , XM_009429844.3 , XM_016927437.2 , XM_009429842.3
Accession Version	XM_009429842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009429842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009428117.1
CDS	589..4116
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_009429842.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009429842.3

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC CGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_009429842.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009428117.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

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CloneID	OPc69099
Clone ID Related Accession (Same CDS sequence)	XM_009429842.2 , XM_016927436.1 , XM_009429844.2 , XM_016927437.1
Accession Version	XM_009429842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009429842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009428117.1
CDS	600..4127
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_009429842.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009429842.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_016927436.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016782925.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc69099
Clone ID Related Accession (Same CDS sequence)	XM_009429842.2 , XM_016927436.1 , XM_009429844.2 , XM_016927437.1
Accession Version	XM_016927436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016782925.1
CDS	365..3892
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_016927436.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927436.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_009429844.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009428119.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

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CloneID	OPc69099
Clone ID Related Accession (Same CDS sequence)	XM_009429842.2 , XM_016927436.1 , XM_009429844.2 , XM_016927437.1
Accession Version	XM_009429844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009429844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009428119.1
CDS	289..3816
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_009429844.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009429844.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_016927437.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016782926.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

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CloneID	OPc69099
Clone ID Related Accession (Same CDS sequence)	XM_009429842.2 , XM_016927436.1 , XM_009429844.2 , XM_016927437.1
Accession Version	XM_016927437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016782926.1
CDS	223..3750
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEPDFFSKLAAMFRGLIFHSSRSGNREVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_016927437.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927437.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGCCTGAC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAGCGGT 
AACCGAGAGG TCCAAGATAG CCACTCTACC AGCTACCCAT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACGCCCC GGCGCTCGCT CTGCCTGTAG CCAGGACGCA GCTCCCAGGC 
CCTGGTCTGC GCTCATTTCA TCCTCTGGCT TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTACGTA CCCTCCAGGC TGAGGAGGAC ACCCTGCCCT CGGCGGAGAC TGCACTCATC 
TTACACCGCA AGGGTTTTGA CTGCGGCCTG GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACAAGCCAAG GCAAGGTAGC CCTGGGCAGC CTTTTCCATG GCCTGGATGT GGTATTCCTT 
CAGCCAACCT CCTTGACGTT ACTGTACCCT CTGGCCTCCC CGTCCAACAG CACTGACGTC 
TATTTGGAGC CCATGGAGAT TGCTACCTTT CGCCTCCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_016927438.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016782927.1 Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPc69100
Clone ID Related Accession (Same CDS sequence)	XM_016927438.1
Accession Version	XM_016927438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA 
GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ACGCAGCTCC CAGGCCCTGG TCTGCGCTCA 
TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACCCTC 
CAGGCTGAGG AGGACACCCT GCCCTCGGCG GAGACTGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG 
GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG 
ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG 
GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016782927.1
CDS	221..3673
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPPEPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVAERAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEESQPLAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHGRQLSISRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKLYVPKEPPVLRVTEGPFFSEVVAYYEHIHQAVRLYNLPGVEGLSLDISSLVDIRDYVNKELALRIHTDIGSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQKRLTLHTAQALGVSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEVQDSHSTSYPSLLSHLTSMYLNAPALALPVARTQLPGPGLRSFHPLASSLPCDFHLLNLRTLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYLEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_016927438.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016927438.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCCA TCTTCTGTGT GGCAGTCTTC 
TCGCTCTACC TCATGCTGGA CCGAGTGCAA CACGATCCCA CCCGACACCA GAATGGTGGG 
AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTT 
TTGGAGGAGA ACCATGAGAT TATCAGCCAT ATCAAGGACT CCGTGCTGGA GCTGACAGCC 
AACGCAGAGG GCCCGCCCGC CATGCTGCCC TACTACACGG TCAATGGCTC CTGGGTGGTG 
CCACCGGAGC CCCGGCCAAG CTTCTTCTCC ATCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAACTGCAG ATGCTCACTG TGTCGGAGGA GCTGCCGTTT 
GACAACGTGG ATGGTGGTGT GTGGAGGCAA GGCTTCGACA TCTCCTACGA CCCGCACGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCCCACT CTCACAATGA CCCAGGCTGG 
ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAAC ACATCCTCAA TAGCATGGTG 
TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CAGAGGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAA TGTCCAAAAG AGAGCGGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCGAC AGGAGGCTGG GTGATGCCAG ATGAGGCCAA TTCCCACTAC 
TTTGCATTGA TTGACCAGCT CATCGAAGGA CACCAGTGGC TGGAGAGAAA TCTTGGTGCA 
ACCCCCCGCT CTGGCTGGGC AGTGGACCCC TTTGGATACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTCAC CAGCATGCTG ATTCAGAGAG TGCACTATGC CATCAAGAAG 
CACTTTGCTG CCACCCACAG CCTAGAGTTC ATGTGGAGGC AGACATGGGA CTCGGACTCC 
AGCACAGACA TCTTCTGTCA CATGATGCCC TTCTACAGCT ATGACGTCCC CCATACCTGT 
GGCCCGGATC CCAAGATCTG CTGCCAATTT GATTTCAAAC GCCTGCCTGG TGGGCGCATC 
AACTGCCCTT GGAAGGTGCC ACCCCGGGCC ATCACAGAGG CCAACGTGGC AGAGAGGGCA 
GCCCTGCTTC TGGACCAATA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTG 
GTGCCTCTTG GAGATGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCTTCAAC AGCAGGCCTA ACCTCCATGT GCAGGCCCAG 
TTTGGCACTC TTTCTGACTA TTTTGATGCC CTGTACAAGA GGACAGGGGT GGAGCCAGGG 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGC GGGGATTTCT TCTCCTATGC GGACCGGGAG 
GATCATTACT GGACAGGCTA TTACACTTCC CGGCCCTTCT ACAAGAGCTT AGACCGAGTC 
CTGGAAGCCC ACCTGCGGGG GGCAGAGGTT CTGTACAGCC TGGCTGCAGC TCACGCTCGC 
CGCTCTGGTC TGGCTGGCCG GTACCCACTG TCTGATTTCA CCCTCCTGAC GGAAGCTCGG 
CGCACACTGG GGCTCTTCCA GCATCACGAT GCCATCACTG GCACGGCCAA GGAGGCTGTG 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA GGTCATCATT 
CATGCAGCCC ACTATCTGGT GCTGGGGGAC AAGGAGACCT ACCACTTTGA CCCTGAGGCG 
CCCTTCCTCC AAGTGGATGA CACTCGCTTA AGTCACGACG CCCTCCCAGA GCGCACGGTG 
ATCCAGCTGG ATTCGTCGCC CAGGTTTGTG GTCCTATTCA ACCCACTGGA ACAGGAGCGA 
TTCAGCATGG TGTCCCTGCT GGTCAACTCT CCCCGCGTGC GTGTCCTTTC GGAGGAGAGT 
CAGCCCCTGG CCGTGCAGAT CAGCGCACAC TGGAGCTCTG CCACCGAGGC GGTCCCTGAC 
GTCTACCAGG TGTCTGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGCGT GCTGCAGCTA 
CAGCTGGGCC TGGATGGGCA CCGCACGCTG CCCTCCTCTG TGCGCATCTA CCTGCACGGC 
CGGCAGCTGT CCATCAGCAG GCACGAAGCG TTTCCTCTCC GTGTCATTGA CTCTGGCACC 
AGCGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACTGGGCTC 
CTCAAGAGCA TCCGAAGGGT GGATGAGGAG CACGAGCAGC AGGTGGACAT GCAGGTCCTT 
GTCTATGGCA CCCGTACGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCCGATGGC 
GAGGCCAAGC TCTACGTCCC CAAGGAGCCC CCCGTGCTGC GTGTCACTGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCGTA CTACGAGCAC ATTCACCAGG CGGTCCGGCT TTACAATCTG 
CCAGGGGTGG AGGGGCTGTC TCTGGACATA TCATCCCTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATCCATACA GACATCGGCA GCCAGGGTAT CTTCTTCACA 
GACCTCAATG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTCCC CCTCCAGGCC 
AACTTCTACC CCATGCCAGT CATGGCCTAT ATCCAGGACG CACAGAAGCG CCTCACGCTG 
CACACTGCCC AGGCCCTGGG TGTCTCTAGC CTCAAAGATG GCCAGCTGGA GGTGATCTTG 
GACCGGCGGC TGATGCAGGA TGACAACCGG GGCCTAGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCGTTTCCG CCTCCTGCTA GAGCGGCGAA CCGTGGGCAG TGAGGTCCAA 
GATAGCCACT CTACCAGCTA CCCATCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
GCCCCGGCGC TCGCTCTGCC TGTAGCCAGG ACGCAGCTCC CAGGCCCTGG TCTGCGCTCA 
TTTCATCCTC TGGCTTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT ACGTACCCTC 
CAGGCTGAGG AGGACACCCT GCCCTCGGCG GAGACTGCAC TCATCTTACA CCGCAAGGGT 
TTTGACTGCG GCCTGGAGGC CAAGAACTTG GGCTTCAACT GCACCACAAG CCAAGGCAAG 
GTAGCCCTGG GCAGCCTTTT CCATGGCCTG GATGTGGTAT TCCTTCAGCC AACCTCCTTG 
ACGTTACTGT ACCCTCTGGC CTCCCCGTCC AACAGCACTG ACGTCTATTT GGAGCCCATG 
GAGATTGCTA CCTTTCGCCT CCGCTTGGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAN2A2
Entrez Gene ID	745986
Full Name	mannosidase alpha class 2A member 2
General protein information	Preferred Names mannosidase alpha class 2A member 2 Names alpha-mannosidase 2x
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Oryza sativa (rice)	Os06g0245700	NP_001057277.2
Rattus norvegicus (Norway rat)	Man2a2	NP_001100997.1
Xenopus tropicalis (tropical clawed frog)	man2a2	NP_001006777.1
Pan troglodytes (chimpanzee)	MAN2A2	XP_001169951.1
Gallus gallus (chicken)	MAN2A2	XP_001233608.2
Danio rerio (zebrafish)	man2a2	XP_005163040.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004020	XP_318477.4
Homo sapiens (human)	MAN2A2	NP_006113.2
Bos taurus (cattle)	MAN2A2	NP_001179570.1
Mus musculus (house mouse)	Man2a2	NP_766491.2
Drosophila melanogaster (fruit fly)	alpha-Man-II	NP_524291.2
Canis lupus familiaris (dog)	MAN2A2	XP_545863.3
Arabidopsis thaliana (thale cress)	GMII	NP_196999.1
Caenorhabditis elegans (roundworm)	aman-2	NP_505995.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAN2A2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

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