aman-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	aman-2
Entrez Gene ID	179632
Full Name	Alpha-mannosidase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_073594.4	NP_505995.2	Alpha-mann_mid domain-containing protein
NM_073594.3	NP_505995.2	Alpha-mannosidase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0245700	NP_001057277.2
Rattus norvegicus (Norway rat)	Man2a2	NP_001100997.1
Xenopus tropicalis (tropical clawed frog)	man2a2	NP_001006777.1
Pan troglodytes (chimpanzee)	MAN2A2	XP_001169951.1
Gallus gallus (chicken)	MAN2A2	XP_001233608.2
Danio rerio (zebrafish)	man2a2	XP_005163040.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004020	XP_318477.4
Homo sapiens (human)	MAN2A2	NP_006113.2
Bos taurus (cattle)	MAN2A2	NP_001179570.1
Mus musculus (house mouse)	Man2a2	NP_766491.2
Drosophila melanogaster (fruit fly)	alpha-Man-II	NP_524291.2
Canis lupus familiaris (dog)	MAN2A2	XP_545863.3
Arabidopsis thaliana (thale cress)	GMII	NP_196999.1
Caenorhabditis elegans (roundworm)	aman-2	NP_505995.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following aman-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aman-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa346613	NM_073594.4 Latest version!	Caenorhabditis elegans Alpha-mann_mid domain-containing protein (aman-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa346613	NM_073594.3	Caenorhabditis elegans Alpha-mannosidase (aman-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aman-2 ( NM_073594.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_505995.2 Caenorhabditis elegans Alpha-mann_mid domain-containing protein (aman-2), mRNA.

Price & Availability↑

CloneID	OCa346613
Clone ID Related Accession (Same CDS sequence)	NM_073594.4 , NM_073594.3
Accession Version	NM_073594.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Caenorhabditis elegans
Product	Alpha-mann_mid domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_073594.3.

ATGGGAAAAC GCAATTTCTA TATTATCCTA TGTTTGGGAG TCTTTCTCAC CGTATCACTC 
TATTTGTACA ATGGAATTGA AACCGGAGCT GAAGCGCTCA CCAAACGACA AAGATATGTA 
GATGATTTAC GGCGGAAAAT CGGAAATTTG GAGCATGTAG CAGAAGAAAA TGGAAGAACG 
ATAGACCGCT TGGAACAAGA AGTTCAACGA GCAAAAGCTG AAAAATCGGT AGATTTTGAT 
GAAGAAAAAG AAAAAACGGA AGAAAAAGAA GTAGAAAAAG AGGAAAAAGA AGTTGCACCA 
GTTCCAGTTC GAGGAAATCG TGGTGAAATG GCTCATATTC ATCAAGTAAA GCAACATATC 
AAGCCAACTC CATCGATGAA AGATGTTTGT GGAATTAGAG AAAACGTCAG CATTGCTCAT 
TCAGACCTGC AGATGCTCGA TCTCTATGAC ACCTGGAAGT TCGAAAATCC AGACGGAGGT 
GTATGGAAAC AAGGATGGAA AATTGAATAC GATGCAGAGA AAGTCAAATC TCTTCCACGT 
TTGGAAGTTA TTGTGATACC TCATTCTCAT TGTGATCCCG GATGGATTAT GACTTTCGAA 
GAGTATTACA ACAGACAAAC TCGCAATATT CTTGATGGAA TGGCTAAACA TTTGGCAGAA 
AAAGACGAAA TGCGGTTTAT ATATGCAGAA ATATCATTTT TCGAAACTTG GTGGAGAGAC 
CAGGCAGATG AAATTAAAAA GAAAGTTAAA GGATATTTGG AAGCAGGAAA GTTTGAAATT 
GTTACTGGCG GATGGGTTAT GACAGATGAA GCTAATGCAC ATTATCACTC AATGATCACT 
GAATTGTTCG AAGGACATGA ATGGATTCAA AATCATTTGG GAAAAAGCGC CATTCCACAA 
TCTCATTGGT CAATTGATCC ATTCGGTTTA TCACCATCAA TGCCACATCT TCTAACTTCT 
GCTAATATAA CCAATGCTGT AATTCAAAGA GTTCATTATT CGGTGAAACG TGAGCTTGCT 
CTGAAAAAGA ATCTTGAATT CTACTGGAGA CAATTATTTG GATCAACTGG ACATCCTGAT 
CTTCGTTCAC ATATTATGCC TTTCTACTCT TACGATATAC CTCATACGTG TGGCCCAGAA 
CCGTCTGTTT GCTGTCAATT CGATTTCCGT AGAATGCCAG AAGGTGGAAA ATCATGTGAT 
TGGGGAATCC CTCCACAGAA AATTAACGAT GACAATGTGG CTCACAGAGC TGAAATGATT 
TATGATCAAT ATAGAAAGAA AAGTCAACTT TTCAAGAATA ATGTGATTTT CCAACCACTT 
GGAGATGATT TCAGGTACGA CATTGATTTT GAATGGAATT CACAATATGA AAACTATAAG 
AAATTGTTCG AATACATGAA TTCCAAATCA GAATGGAATG TTCATGCTCA ATTCGGAACT 
CTTTCTGATT ATTTCAAGAA GCTTGATACT GCAATTTCTG CGTCTGGCGA GCAACTTCCA 
ACTTTTTCTG GAGATTTCTT CACTTATGCG GACAGAGATG ATCATTATTG GAGTGGATAC 
TTCACTTCCC GTCCATTCTA TAAACAGCTT GATCGGGTTC TCCAACATTA TTTAAGATCA 
GCTGAAATCG CCTTTACCCT TGCAAATATT GAAGAAGAAG GAATGGTTGA AGCGAAAATT 
TTTGAGAAGC TTGTGACTGC TCGACGAGCT CTTTCACTTT TCCAACATCA CGATGGTGTA 
ACTGGTACGG CAAAAGATCA CGTCGTCTTG GATTATGGTC AGAAAATGAT TGATGCTTTG 
AACGCATGTG AGGATATTCT TTCGGAAGCT CTTGTTGTAT TGCTGGGAAT TGATTCAACG 
AATAAGATGC AGATGGATGA GCATAGAGTT AATGAAAACC TTCTACCCGA AAAACGTGTC 
TATAAAATTG GGCAAAACGT CGTATTGTTC AATACTTTAT CTAGAAATCG CAACGAGCCA 
ATTTGTATTC AAGTTGATTC TCTTGACGCT GGTGTCGAAG CTGATCCTCC AATTAAAAAA 
CAACAAGTTT CGCCGGTTAT TGCATATGAT GAAGAGAAGA AAACGCTTGT TGTCAAAAAC 
GGAATATTCG AACTTTGCTT CATGTTATCA CTTGGACCAA TGGAGTCTGT CAGTTTCAGA 
CTTGTGAAAA ATACAACAAC ATCCAAAGTT GAAATAATCA CCAATAATGC GGCAGAATTC 
AAAGAAACAA GTTTTAAATC TTCATCCACT TCTGGAGACT TTACTGTGAA AAACGACAAA 
GTTGAAGCTG AATTTGATGG AGAAAATGGA ATGATTAAAA GAGCTACCAG TCTTGTTGAT 
GATAAACCAA TTGATTTGAA TTCTCACTTT ATTCATTATG GAGCACGGAA GTCAAAGAGA 
AAGTTCGCAA ATGGAAATGA AGACAACCCG GCTGGCGCAT ACCTGTTCCT TCCCGATGGA 
GAAGCTAGAG AACTCAAAAA ACAATCAAGT GATTGGATAT TGGTAAAAGG AGAAGTTGTT 
CAAAAAGTGT TTGCAACTCC AAACAATGAT CTGAAAATAT TGCAAACGTA CACACTTTAT 
CAAGGGCTTC CATGGATTGA TTTGGATAAT GAAGTTGATG TACGTTCCAA GGAGAATTTC 
GAGTTGGCAC TGAGATTCAG TTCTTCAGTA AATAGTGGTG ATGAGTTTTT CACTGATCTC 
AATGGAATGC AAATGATAAA AAGGAGACGA CAAACTAAAT TACCAACACA GGCCAATTTC 
TATCCCATGT CTGCTGGTGT TTACATTGAA GACGATACTA CCAGAATGTC AATTCATTCG 
GCACAGGCTC TCGGAGTTAG CAGTCTCTCG TCGGGACAAA TTGAAATAAT GCTTGATCGA 
CGACTTAGTT CAGATGACAA CAGAGGTCTT CAGCAAGGAG TTAGAGACAA CAAACGAACA 
GTTGCACATT TCCGTATTGT TATTGAGCCG ATGTCTTCAT CGAGTGGTAA TAAGAAGGAA 
GAACGAGTTG GATTCCATTC ACATGTTGGT CATCTCGCTA CGTGGTCTCT TCATTATCCT 
CTTGTCAAAA TGATTGGAGA TGCAACACCA AAATCTATTT CTTCGAAAAA TGTGGAACAA 
GAGCTGAACT GTGACCTGCA TCTAGTGACA TTTAGAACAC TGGCATCGCC GACAACTTAC 
GAAGCCAACG AAAGATCTAC GGCAGCTGAG AAGAAAGCAG CGATGGTGAT GCATAGAGTT 
GTTCCAGACT GTAGATCCAG GCTTACCCTC CCAGACACGT CATGCTTAGC TACTGGATTA 
GAAATTGAGC CACTCAAATT GATCTCGACA CTGAAGTCTG CGAAAAAAAC GTCACTAACC 
AATCTTTATG AAGGAAACAA GGCTGAACAA TTCCGACTCC AACCAAACGA TATTTCCAGT 
ATTCTTGTAT CATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_505995.2
CDS	20..3457
Translation	MGKRNFYIILCLGVFLTVSLYLYNGIETGAEALTKRQRYVDDLRRKIGNLEHVAEENGRTIDRLEQEVQRAKAEKSVDFDEEKEKTEEKEVEKEEKEVAPVPVRGNRGEMAHIHQVKQHIKPTPSMKDVCGIRENVSIAHSDLQMLDLYDTWKFENPDGGVWKQGWKIEYDAEKVKSLPRLEVIVIPHSHCDPGWIMTFEEYYNRQTRNILDGMAKHLAEKDEMRFIYAEISFFETWWRDQADEIKKKVKGYLEAGKFEIVTGGWVMTDEANAHYHSMITELFEGHEWIQNHLGKSAIPQSHWSIDPFGLSPSMPHLLTSANITNAVIQRVHYSVKRELALKKNLEFYWRQLFGSTGHPDLRSHIMPFYSYDIPHTCGPEPSVCCQFDFRRMPEGGKSCDWGIPPQKINDDNVAHRAEMIYDQYRKKSQLFKNNVIFQPLGDDFRYDIDFEWNSQYENYKKLFEYMNSKSEWNVHAQFGTLSDYFKKLDTAISASGEQLPTFSGDFFTYADRDDHYWSGYFTSRPFYKQLDRVLQHYLRSAEIAFTLANIEEEGMVEAKIFEKLVTARRALSLFQHHDGVTGTAKDHVVLDYGQKMIDALNACEDILSEALVVLLGIDSTNKMQMDEHRVNENLLPEKRVYKIGQNVVLFNTLSRNRNEPICIQVDSLDAGVEADPPIKKQQVSPVIAYDEEKKTLVVKNGIFELCFMLSLGPMESVSFRLVKNTTTSKVEIITNNAAEFKETSFKSSSTSGDFTVKNDKVEAEFDGENGMIKRATSLVDDKPIDLNSHFIHYGARKSKRKFANGNEDNPAGAYLFLPDGEARELKKQSSDWILVKGEVVQKVFATPNNDLKILQTYTLYQGLPWIDLDNEVDVRSKENFELALRFSSSVNSGDEFFTDLNGMQMIKRRRQTKLPTQANFYPMSAGVYIEDDTTRMSIHSAQALGVSSLSSGQIEIMLDRRLSSDDNRGLQQGVRDNKRTVAHFRIVIEPMSSSSGNKKEERVGFHSHVGHLATWSLHYPLVKMIGDATPKSISSKNVEQELNCDLHLVTFRTLASPTTYEANERSTAAEKKAAMVMHRVVPDCRSRLTLPDTSCLATGLEIEPLKLISTLKSAKKTSLTNLYEGNKAEQFRLQPNDISSILVSF

Target ORF information:

RefSeq Version	NM_073594.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Alpha-mann_mid domain-containing protein (aman-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_073594.4

ORF Insert Sequence:

ATGGGAAAAC GCAATTTCTA TATTATCCTA TGTTTGGGAG TCTTTCTCAC CGTATCACTC 
TATTTGTACA ATGGAATTGA AACCGGAGCT GAAGCGCTCA CCAAACGACA AAGATATGTA 
GATGATTTAC GGCGGAAAAT CGGAAATTTG GAGCATGTAG CAGAAGAAAA TGGAAGAACG 
ATAGACCGCT TGGAACAAGA AGTTCAACGA GCAAAAGCTG AAAAATCGGT AGATTTTGAT 
GAAGAAAAAG AAAAAACGGA AGAAAAAGAA GTAGAAAAAG AGGAAAAAGA AGTTGCACCA 
GTTCCAGTTC GAGGAAATCG TGGTGAAATG GCTCATATTC ATCAAGTAAA GCAACATATC 
AAGCCAACTC CATCGATGAA AGATGTTTGT GGAATTAGAG AAAACGTCAG CATTGCTCAT 
TCAGACCTGC AGATGCTCGA TCTCTATGAC ACCTGGAAGT TCGAAAATCC AGACGGAGGT 
GTATGGAAAC AAGGATGGAA AATTGAATAC GATGCAGAGA AAGTCAAATC TCTTCCACGT 
TTGGAAGTTA TTGTGATACC TCATTCTCAT TGTGATCCCG GATGGATTAT GACTTTCGAA 
GAGTATTACA ACAGACAAAC TCGCAATATT CTTGATGGAA TGGCTAAACA TTTGGCAGAA 
AAAGACGAAA TGCGGTTTAT ATATGCAGAA ATATCATTTT TCGAAACTTG GTGGAGAGAC 
CAGGCAGATG AAATTAAAAA GAAAGTTAAA GGATATTTGG AAGCAGGAAA GTTTGAAATT 
GTTACTGGCG GATGGGTTAT GACAGATGAA GCTAATGCAC ATTATCACTC AATGATCACT 
GAATTGTTCG AAGGACATGA ATGGATTCAA AATCATTTGG GAAAAAGCGC CATTCCACAA 
TCTCATTGGT CAATTGATCC ATTCGGTTTA TCACCATCAA TGCCACATCT TCTAACTTCT 
GCTAATATAA CCAATGCTGT AATTCAAAGA GTTCATTATT CGGTGAAACG TGAGCTTGCT 
CTGAAAAAGA ATCTTGAATT CTACTGGAGA CAATTATTTG GATCAACTGG ACATCCTGAT 
CTTCGTTCAC ATATTATGCC TTTCTACTCT TACGATATAC CTCATACGTG TGGCCCAGAA 
CCGTCTGTTT GCTGTCAATT CGATTTCCGT AGAATGCCAG AAGGTGGAAA ATCATGTGAT 
TGGGGAATCC CTCCACAGAA AATTAACGAT GACAATGTGG CTCACAGAGC TGAAATGATT 
TATGATCAAT ATAGAAAGAA AAGTCAACTT TTCAAGAATA ATGTGATTTT CCAACCACTT 
GGAGATGATT TCAGGTACGA CATTGATTTT GAATGGAATT CACAATATGA AAACTATAAG 
AAATTGTTCG AATACATGAA TTCCAAATCA GAATGGAATG TTCATGCTCA ATTCGGAACT 
CTTTCTGATT ATTTCAAGAA GCTTGATACT GCAATTTCTG CGTCTGGCGA GCAACTTCCA 
ACTTTTTCTG GAGATTTCTT CACTTATGCG GACAGAGATG ATCATTATTG GAGTGGATAC 
TTCACTTCCC GTCCATTCTA TAAACAGCTT GATCGGGTTC TCCAACATTA TTTAAGATCA 
GCTGAAATCG CCTTTACCCT TGCAAATATT GAAGAAGAAG GAATGGTTGA AGCGAAAATT 
TTTGAGAAGC TTGTGACTGC TCGACGAGCT CTTTCACTTT TCCAACATCA CGATGGTGTA 
ACTGGTACGG CAAAAGATCA CGTCGTCTTG GATTATGGTC AGAAAATGAT TGATGCTTTG 
AACGCATGTG AGGATATTCT TTCGGAAGCT CTTGTTGTAT TGCTGGGAAT TGATTCAACG 
AATAAGATGC AGATGGATGA GCATAGAGTT AATGAAAACC TTCTACCCGA AAAACGTGTC 
TATAAAATTG GGCAAAACGT CGTATTGTTC AATACTTTAT CTAGAAATCG CAACGAGCCA 
ATTTGTATTC AAGTTGATTC TCTTGACGCT GGTGTCGAAG CTGATCCTCC AATTAAAAAA 
CAACAAGTTT CGCCGGTTAT TGCATATGAT GAAGAGAAGA AAACGCTTGT TGTCAAAAAC 
GGAATATTCG AACTTTGCTT CATGTTATCA CTTGGACCAA TGGAGTCTGT CAGTTTCAGA 
CTTGTGAAAA ATACAACAAC ATCCAAAGTT GAAATAATCA CCAATAATGC GGCAGAATTC 
AAAGAAACAA GTTTTAAATC TTCATCCACT TCTGGAGACT TTACTGTGAA AAACGACAAA 
GTTGAAGCTG AATTTGATGG AGAAAATGGA ATGATTAAAA GAGCTACCAG TCTTGTTGAT 
GATAAACCAA TTGATTTGAA TTCTCACTTT ATTCATTATG GAGCACGGAA GTCAAAGAGA 
AAGTTCGCAA ATGGAAATGA AGACAACCCG GCTGGCGCAT ACCTGTTCCT TCCCGATGGA 
GAAGCTAGAG AACTCAAAAA ACAATCAAGT GATTGGATAT TGGTAAAAGG AGAAGTTGTT 
CAAAAAGTGT TTGCAACTCC AAACAATGAT CTGAAAATAT TGCAAACGTA CACACTTTAT 
CAAGGGCTTC CATGGATTGA TTTGGATAAT GAAGTTGATG TACGTTCCAA GGAGAATTTC 
GAGTTGGCAC TGAGATTCAG TTCTTCAGTA AATAGTGGTG ATGAGTTTTT CACTGATCTC 
AATGGAATGC AAATGATAAA AAGGAGACGA CAAACTAAAT TACCAACACA GGCCAATTTC 
TATCCCATGT CTGCTGGTGT TTACATTGAA GACGATACTA CCAGAATGTC AATTCATTCG 
GCACAGGCTC TCGGAGTTAG CAGTCTCTCG TCGGGACAAA TTGAAATAAT GCTTGATCGA 
CGACTTAGTT CAGATGACAA CAGAGGTCTT CAGCAAGGAG TTAGAGACAA CAAACGAACA 
GTTGCACATT TCCGTATTGT TATTGAGCCG ATGTCTTCAT CGAGTGGTAA TAAGAAGGAA 
GAACGAGTTG GATTCCATTC ACATGTTGGT CATCTCGCTA CGTGGTCTCT TCATTATCCT 
CTTGTCAAAA TGATTGGAGA TGCAACACCA AAATCTATTT CTTCGAAAAA TGTGGAACAA 
GAGCTGAACT GTGACCTGCA TCTAGTGACA TTTAGAACAC TGGCATCGCC GACAACTTAC 
GAAGCCAACG AAAGATCTAC GGCAGCTGAG AAGAAAGCAG CGATGGTGAT GCATAGAGTT 
GTTCCAGACT GTAGATCCAG GCTTACCCTC CCAGACACGT CATGCTTAGC TACTGGATTA 
GAAATTGAGC CACTCAAATT GATCTCGACA CTGAAGTCTG CGAAAAAAAC GTCACTAACC 
AATCTTTATG AAGGAAACAA GGCTGAACAA TTCCGACTCC AACCAAACGA TATTTCCAGT 
ATTCTTGTAT CATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aman-2 ( NM_073594.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_505995.2 Caenorhabditis elegans Alpha-mannosidase (aman-2), partial mRNA.

Price & Availability↑

CloneID	OCa346613
Clone ID Related Accession (Same CDS sequence)	NM_073594.4 , NM_073594.3
Accession Version	NM_073594.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554652800000
Organism	Caenorhabditis elegans
Product	Alpha-mannosidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_073594.2. COMPLETENESS: incomplete on both ends.

ATGGGAAAAC GCAATTTCTA TATTATCCTA TGTTTGGGAG TCTTTCTCAC CGTATCACTC 
TATTTGTACA ATGGAATTGA AACCGGAGCT GAAGCGCTCA CCAAACGACA AAGATATGTA 
GATGATTTAC GGCGGAAAAT CGGAAATTTG GAGCATGTAG CAGAAGAAAA TGGAAGAACG 
ATAGACCGCT TGGAACAAGA AGTTCAACGA GCAAAAGCTG AAAAATCGGT AGATTTTGAT 
GAAGAAAAAG AAAAAACGGA AGAAAAAGAA GTAGAAAAAG AGGAAAAAGA AGTTGCACCA 
GTTCCAGTTC GAGGAAATCG TGGTGAAATG GCTCATATTC ATCAAGTAAA GCAACATATC 
AAGCCAACTC CATCGATGAA AGATGTTTGT GGAATTAGAG AAAACGTCAG CATTGCTCAT 
TCAGACCTGC AGATGCTCGA TCTCTATGAC ACCTGGAAGT TCGAAAATCC AGACGGAGGT 
GTATGGAAAC AAGGATGGAA AATTGAATAC GATGCAGAGA AAGTCAAATC TCTTCCACGT 
TTGGAAGTTA TTGTGATACC TCATTCTCAT TGTGATCCCG GATGGATTAT GACTTTCGAA 
GAGTATTACA ACAGACAAAC TCGCAATATT CTTGATGGAA TGGCTAAACA TTTGGCAGAA 
AAAGACGAAA TGCGGTTTAT ATATGCAGAA ATATCATTTT TCGAAACTTG GTGGAGAGAC 
CAGGCAGATG AAATTAAAAA GAAAGTTAAA GGATATTTGG AAGCAGGAAA GTTTGAAATT 
GTTACTGGCG GATGGGTTAT GACAGATGAA GCTAATGCAC ATTATCACTC AATGATCACT 
GAATTGTTCG AAGGACATGA ATGGATTCAA AATCATTTGG GAAAAAGCGC CATTCCACAA 
TCTCATTGGT CAATTGATCC ATTCGGTTTA TCACCATCAA TGCCACATCT TCTAACTTCT 
GCTAATATAA CCAATGCTGT AATTCAAAGA GTTCATTATT CGGTGAAACG TGAGCTTGCT 
CTGAAAAAGA ATCTTGAATT CTACTGGAGA CAATTATTTG GATCAACTGG ACATCCTGAT 
CTTCGTTCAC ATATTATGCC TTTCTACTCT TACGATATAC CTCATACGTG TGGCCCAGAA 
CCGTCTGTTT GCTGTCAATT CGATTTCCGT AGAATGCCAG AAGGTGGAAA ATCATGTGAT 
TGGGGAATCC CTCCACAGAA AATTAACGAT GACAATGTGG CTCACAGAGC TGAAATGATT 
TATGATCAAT ATAGAAAGAA AAGTCAACTT TTCAAGAATA ATGTGATTTT CCAACCACTT 
GGAGATGATT TCAGGTACGA CATTGATTTT GAATGGAATT CACAATATGA AAACTATAAG 
AAATTGTTCG AATACATGAA TTCCAAATCA GAATGGAATG TTCATGCTCA ATTCGGAACT 
CTTTCTGATT ATTTCAAGAA GCTTGATACT GCAATTTCTG CGTCTGGCGA GCAACTTCCA 
ACTTTTTCTG GAGATTTCTT CACTTATGCG GACAGAGATG ATCATTATTG GAGTGGATAC 
TTCACTTCCC GTCCATTCTA TAAACAGCTT GATCGGGTTC TCCAACATTA TTTAAGATCA 
GCTGAAATCG CCTTTACCCT TGCAAATATT GAAGAAGAAG GAATGGTTGA AGCGAAAATT 
TTTGAGAAGC TTGTGACTGC TCGACGAGCT CTTTCACTTT TCCAACATCA CGATGGTGTA 
ACTGGTACGG CAAAAGATCA CGTCGTCTTG GATTATGGTC AGAAAATGAT TGATGCTTTG 
AACGCATGTG AGGATATTCT TTCGGAAGCT CTTGTTGTAT TGCTGGGAAT TGATTCAACG 
AATAAGATGC AGATGGATGA GCATAGAGTT AATGAAAACC TTCTACCCGA AAAACGTGTC 
TATAAAATTG GGCAAAACGT CGTATTGTTC AATACTTTAT CTAGAAATCG CAACGAGCCA 
ATTTGTATTC AAGTTGATTC TCTTGACGCT GGTGTCGAAG CTGATCCTCC AATTAAAAAA 
CAACAAGTTT CGCCGGTTAT TGCATATGAT GAAGAGAAGA AAACGCTTGT TGTCAAAAAC 
GGAATATTCG AACTTTGCTT CATGTTATCA CTTGGACCAA TGGAGTCTGT CAGTTTCAGA 
CTTGTGAAAA ATACAACAAC ATCCAAAGTT GAAATAATCA CCAATAATGC GGCAGAATTC 
AAAGAAACAA GTTTTAAATC TTCATCCACT TCTGGAGACT TTACTGTGAA AAACGACAAA 
GTTGAAGCTG AATTTGATGG AGAAAATGGA ATGATTAAAA GAGCTACCAG TCTTGTTGAT 
GATAAACCAA TTGATTTGAA TTCTCACTTT ATTCATTATG GAGCACGGAA GTCAAAGAGA 
AAGTTCGCAA ATGGAAATGA AGACAACCCG GCTGGCGCAT ACCTGTTCCT TCCCGATGGA 
GAAGCTAGAG AACTCAAAAA ACAATCAAGT GATTGGATAT TGGTAAAAGG AGAAGTTGTT 
CAAAAAGTGT TTGCAACTCC AAACAATGAT CTGAAAATAT TGCAAACGTA CACACTTTAT 
CAAGGGCTTC CATGGATTGA TTTGGATAAT GAAGTTGATG TACGTTCCAA GGAGAATTTC 
GAGTTGGCAC TGAGATTCAG TTCTTCAGTA AATAGTGGTG ATGAGTTTTT CACTGATCTC 
AATGGAATGC AAATGATAAA AAGGAGACGA CAAACTAAAT TACCAACACA GGCCAATTTC 
TATCCCATGT CTGCTGGTGT TTACATTGAA GACGATACTA CCAGAATGTC AATTCATTCG 
GCACAGGCTC TCGGAGTTAG CAGTCTCTCG TCGGGACAAA TTGAAATAAT GCTTGATCGA 
CGACTTAGTT CAGATGACAA CAGAGGTCTT CAGCAAGGAG TTAGAGACAA CAAACGAACA 
GTTGCACATT TCCGTATTGT TATTGAGCCG ATGTCTTCAT CGAGTGGTAA TAAGAAGGAA 
GAACGAGTTG GATTCCATTC ACATGTTGGT CATCTCGCTA CGTGGTCTCT TCATTATCCT 
CTTGTCAAAA TGATTGGAGA TGCAACACCA AAATCTATTT CTTCGAAAAA TGTGGAACAA 
GAGCTGAACT GTGACCTGCA TCTAGTGACA TTTAGAACAC TGGCATCGCC GACAACTTAC 
GAAGCCAACG AAAGATCTAC GGCAGCTGAG AAGAAAGCAG CGATGGTGAT GCATAGAGTT 
GTTCCAGACT GTAGATCCAG GCTTACCCTC CCAGACACGT CATGCTTAGC TACTGGATTA 
GAAATTGAGC CACTCAAATT GATCTCGACA CTGAAGTCTG CGAAAAAAAC GTCACTAACC 
AATCTTTATG AAGGAAACAA GGCTGAACAA TTCCGACTCC AACCAAACGA TATTTCCAGT 
ATTCTTGTAT CATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_505995.2
CDS	1..3438
Translation	MGKRNFYIILCLGVFLTVSLYLYNGIETGAEALTKRQRYVDDLRRKIGNLEHVAEENGRTIDRLEQEVQRAKAEKSVDFDEEKEKTEEKEVEKEEKEVAPVPVRGNRGEMAHIHQVKQHIKPTPSMKDVCGIRENVSIAHSDLQMLDLYDTWKFENPDGGVWKQGWKIEYDAEKVKSLPRLEVIVIPHSHCDPGWIMTFEEYYNRQTRNILDGMAKHLAEKDEMRFIYAEISFFETWWRDQADEIKKKVKGYLEAGKFEIVTGGWVMTDEANAHYHSMITELFEGHEWIQNHLGKSAIPQSHWSIDPFGLSPSMPHLLTSANITNAVIQRVHYSVKRELALKKNLEFYWRQLFGSTGHPDLRSHIMPFYSYDIPHTCGPEPSVCCQFDFRRMPEGGKSCDWGIPPQKINDDNVAHRAEMIYDQYRKKSQLFKNNVIFQPLGDDFRYDIDFEWNSQYENYKKLFEYMNSKSEWNVHAQFGTLSDYFKKLDTAISASGEQLPTFSGDFFTYADRDDHYWSGYFTSRPFYKQLDRVLQHYLRSAEIAFTLANIEEEGMVEAKIFEKLVTARRALSLFQHHDGVTGTAKDHVVLDYGQKMIDALNACEDILSEALVVLLGIDSTNKMQMDEHRVNENLLPEKRVYKIGQNVVLFNTLSRNRNEPICIQVDSLDAGVEADPPIKKQQVSPVIAYDEEKKTLVVKNGIFELCFMLSLGPMESVSFRLVKNTTTSKVEIITNNAAEFKETSFKSSSTSGDFTVKNDKVEAEFDGENGMIKRATSLVDDKPIDLNSHFIHYGARKSKRKFANGNEDNPAGAYLFLPDGEARELKKQSSDWILVKGEVVQKVFATPNNDLKILQTYTLYQGLPWIDLDNEVDVRSKENFELALRFSSSVNSGDEFFTDLNGMQMIKRRRQTKLPTQANFYPMSAGVYIEDDTTRMSIHSAQALGVSSLSSGQIEIMLDRRLSSDDNRGLQQGVRDNKRTVAHFRIVIEPMSSSSGNKKEERVGFHSHVGHLATWSLHYPLVKMIGDATPKSISSKNVEQELNCDLHLVTFRTLASPTTYEANERSTAAEKKAAMVMHRVVPDCRSRLTLPDTSCLATGLEIEPLKLISTLKSAKKTSLTNLYEGNKAEQFRLQPNDISSILVSF

Target ORF information:

RefSeq Version	NM_073594.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Alpha-mannosidase (aman-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_073594.3

ORF Insert Sequence:

ATGGGAAAAC GCAATTTCTA TATTATCCTA TGTTTGGGAG TCTTTCTCAC CGTATCACTC 
TATTTGTACA ATGGAATTGA AACCGGAGCT GAAGCGCTCA CCAAACGACA AAGATATGTA 
GATGATTTAC GGCGGAAAAT CGGAAATTTG GAGCATGTAG CAGAAGAAAA TGGAAGAACG 
ATAGACCGCT TGGAACAAGA AGTTCAACGA GCAAAAGCTG AAAAATCGGT AGATTTTGAT 
GAAGAAAAAG AAAAAACGGA AGAAAAAGAA GTAGAAAAAG AGGAAAAAGA AGTTGCACCA 
GTTCCAGTTC GAGGAAATCG TGGTGAAATG GCTCATATTC ATCAAGTAAA GCAACATATC 
AAGCCAACTC CATCGATGAA AGATGTTTGT GGAATTAGAG AAAACGTCAG CATTGCTCAT 
TCAGACCTGC AGATGCTCGA TCTCTATGAC ACCTGGAAGT TCGAAAATCC AGACGGAGGT 
GTATGGAAAC AAGGATGGAA AATTGAATAC GATGCAGAGA AAGTCAAATC TCTTCCACGT 
TTGGAAGTTA TTGTGATACC TCATTCTCAT TGTGATCCCG GATGGATTAT GACTTTCGAA 
GAGTATTACA ACAGACAAAC TCGCAATATT CTTGATGGAA TGGCTAAACA TTTGGCAGAA 
AAAGACGAAA TGCGGTTTAT ATATGCAGAA ATATCATTTT TCGAAACTTG GTGGAGAGAC 
CAGGCAGATG AAATTAAAAA GAAAGTTAAA GGATATTTGG AAGCAGGAAA GTTTGAAATT 
GTTACTGGCG GATGGGTTAT GACAGATGAA GCTAATGCAC ATTATCACTC AATGATCACT 
GAATTGTTCG AAGGACATGA ATGGATTCAA AATCATTTGG GAAAAAGCGC CATTCCACAA 
TCTCATTGGT CAATTGATCC ATTCGGTTTA TCACCATCAA TGCCACATCT TCTAACTTCT 
GCTAATATAA CCAATGCTGT AATTCAAAGA GTTCATTATT CGGTGAAACG TGAGCTTGCT 
CTGAAAAAGA ATCTTGAATT CTACTGGAGA CAATTATTTG GATCAACTGG ACATCCTGAT 
CTTCGTTCAC ATATTATGCC TTTCTACTCT TACGATATAC CTCATACGTG TGGCCCAGAA 
CCGTCTGTTT GCTGTCAATT CGATTTCCGT AGAATGCCAG AAGGTGGAAA ATCATGTGAT 
TGGGGAATCC CTCCACAGAA AATTAACGAT GACAATGTGG CTCACAGAGC TGAAATGATT 
TATGATCAAT ATAGAAAGAA AAGTCAACTT TTCAAGAATA ATGTGATTTT CCAACCACTT 
GGAGATGATT TCAGGTACGA CATTGATTTT GAATGGAATT CACAATATGA AAACTATAAG 
AAATTGTTCG AATACATGAA TTCCAAATCA GAATGGAATG TTCATGCTCA ATTCGGAACT 
CTTTCTGATT ATTTCAAGAA GCTTGATACT GCAATTTCTG CGTCTGGCGA GCAACTTCCA 
ACTTTTTCTG GAGATTTCTT CACTTATGCG GACAGAGATG ATCATTATTG GAGTGGATAC 
TTCACTTCCC GTCCATTCTA TAAACAGCTT GATCGGGTTC TCCAACATTA TTTAAGATCA 
GCTGAAATCG CCTTTACCCT TGCAAATATT GAAGAAGAAG GAATGGTTGA AGCGAAAATT 
TTTGAGAAGC TTGTGACTGC TCGACGAGCT CTTTCACTTT TCCAACATCA CGATGGTGTA 
ACTGGTACGG CAAAAGATCA CGTCGTCTTG GATTATGGTC AGAAAATGAT TGATGCTTTG 
AACGCATGTG AGGATATTCT TTCGGAAGCT CTTGTTGTAT TGCTGGGAAT TGATTCAACG 
AATAAGATGC AGATGGATGA GCATAGAGTT AATGAAAACC TTCTACCCGA AAAACGTGTC 
TATAAAATTG GGCAAAACGT CGTATTGTTC AATACTTTAT CTAGAAATCG CAACGAGCCA 
ATTTGTATTC AAGTTGATTC TCTTGACGCT GGTGTCGAAG CTGATCCTCC AATTAAAAAA 
CAACAAGTTT CGCCGGTTAT TGCATATGAT GAAGAGAAGA AAACGCTTGT TGTCAAAAAC 
GGAATATTCG AACTTTGCTT CATGTTATCA CTTGGACCAA TGGAGTCTGT CAGTTTCAGA 
CTTGTGAAAA ATACAACAAC ATCCAAAGTT GAAATAATCA CCAATAATGC GGCAGAATTC 
AAAGAAACAA GTTTTAAATC TTCATCCACT TCTGGAGACT TTACTGTGAA AAACGACAAA 
GTTGAAGCTG AATTTGATGG AGAAAATGGA ATGATTAAAA GAGCTACCAG TCTTGTTGAT 
GATAAACCAA TTGATTTGAA TTCTCACTTT ATTCATTATG GAGCACGGAA GTCAAAGAGA 
AAGTTCGCAA ATGGAAATGA AGACAACCCG GCTGGCGCAT ACCTGTTCCT TCCCGATGGA 
GAAGCTAGAG AACTCAAAAA ACAATCAAGT GATTGGATAT TGGTAAAAGG AGAAGTTGTT 
CAAAAAGTGT TTGCAACTCC AAACAATGAT CTGAAAATAT TGCAAACGTA CACACTTTAT 
CAAGGGCTTC CATGGATTGA TTTGGATAAT GAAGTTGATG TACGTTCCAA GGAGAATTTC 
GAGTTGGCAC TGAGATTCAG TTCTTCAGTA AATAGTGGTG ATGAGTTTTT CACTGATCTC 
AATGGAATGC AAATGATAAA AAGGAGACGA CAAACTAAAT TACCAACACA GGCCAATTTC 
TATCCCATGT CTGCTGGTGT TTACATTGAA GACGATACTA CCAGAATGTC AATTCATTCG 
GCACAGGCTC TCGGAGTTAG CAGTCTCTCG TCGGGACAAA TTGAAATAAT GCTTGATCGA 
CGACTTAGTT CAGATGACAA CAGAGGTCTT CAGCAAGGAG TTAGAGACAA CAAACGAACA 
GTTGCACATT TCCGTATTGT TATTGAGCCG ATGTCTTCAT CGAGTGGTAA TAAGAAGGAA 
GAACGAGTTG GATTCCATTC ACATGTTGGT CATCTCGCTA CGTGGTCTCT TCATTATCCT 
CTTGTCAAAA TGATTGGAGA TGCAACACCA AAATCTATTT CTTCGAAAAA TGTGGAACAA 
GAGCTGAACT GTGACCTGCA TCTAGTGACA TTTAGAACAC TGGCATCGCC GACAACTTAC 
GAAGCCAACG AAAGATCTAC GGCAGCTGAG AAGAAAGCAG CGATGGTGAT GCATAGAGTT 
GTTCCAGACT GTAGATCCAG GCTTACCCTC CCAGACACGT CATGCTTAGC TACTGGATTA 
GAAATTGAGC CACTCAAATT GATCTCGACA CTGAAGTCTG CGAAAAAAAC GTCACTAACC 
AATCTTTATG AAGGAAACAA GGCTGAACAA TTCCGACTCC AACCAAACGA TATTTCCAGT 
ATTCTTGTAT CATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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aman-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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