Man2a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man2a2
Entrez Gene ID	140481
Full Name	mannosidase 2, alpha 2
Synonyms	1700052O22Rik,4931438M07Rik,AI480988,MX,Man IIx
General protein information	Preferred Names mannosidase 2, alpha 2 Names alpha-mannosidase 2x alpha-mannosidase IIx mannosidase, alpha, class 2A, member 2 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_172903.4	NP_766491.2	alpha-mannosidase 2x
XM_006540599.3	XP_006540662.1	alpha-mannosidase 2x isoform X1
XM_006540600.4	XP_006540663.1	alpha-mannosidase 2x isoform X2
XM_006540600.3	XP_006540663.1	alpha-mannosidase 2x isoform X2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0245700	NP_001057277.2
Rattus norvegicus (Norway rat)	Man2a2	NP_001100997.1
Xenopus tropicalis (tropical clawed frog)	man2a2	NP_001006777.1
Pan troglodytes (chimpanzee)	MAN2A2	XP_001169951.1
Gallus gallus (chicken)	MAN2A2	XP_001233608.2
Danio rerio (zebrafish)	man2a2	XP_005163040.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004020	XP_318477.4
Homo sapiens (human)	MAN2A2	NP_006113.2
Bos taurus (cattle)	MAN2A2	NP_001179570.1
Mus musculus (house mouse)	Man2a2	NP_766491.2
Drosophila melanogaster (fruit fly)	alpha-Man-II	NP_524291.2
Canis lupus familiaris (dog)	MAN2A2	XP_545863.3
Arabidopsis thaliana (thale cress)	GMII	NP_196999.1
Caenorhabditis elegans (roundworm)	aman-2	NP_505995.2

Gene Ontology
Bibliography

Related articles in PubMed

N-Glycan structure analysis using lectins and an alpha-mannosidase activity assay.
Akama TO, Fukuda MN
Methods in enzymology416304-14(2006)

A genetic approach to Mammalian glycan function.
Lowe JB, Marth JD
Annual review of biochemistry72643-91(2003)

In vivo role of alpha-mannosidase IIx: ineffective spermatogenesis resulting from targeted disruption of the Man2a2 in the mouse.
Fukuda MN, Akama TO
Biochimica et biophysica acta1573(3)382-7(2002 Dec)

Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A spermatogonia.
Hiramoto S, Tamba M, Kiuchi S, Jin YZ, Bannai S, Sugita Y, Dacheux F, Dacheux JL, Yoshida M, Okamura N
Biochemical and biophysical research communications241(2)439-45(1997 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Man2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38661	XM_006540600.4 Latest version!	Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu04272	NM_172903.4 Latest version!	Mus musculus mannosidase 2, alpha 2 (Man2a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu04272	XM_006540599.3 Latest version!	Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu38661	XM_006540600.3	Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man2a2 ( XM_006540600.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540663.1 Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu38661
Clone ID Related Accession (Same CDS sequence)	XM_006540600.4 , XM_006540600.3
Accession Version	XM_006540600.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540600.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA GCCGTGTAAG CCATGATGCC CTGCCCGAGC GCACGGTGAT CCGGCTGGAC 
TCCTCACCCA GGTTTGTGGT GGTATTTAAC CCGCTGGAAC AGGAGCGGCT CAGTGTGGTG 
TCCCTGCTGG TCAACTCGCC CCGGGTGCGC GTCCTTTCAG AGGAGGGCCA GCCCCTGTCT 
GTGCAGATCA GTGTGCACTG GAGCTCGGCT ACGGACATGG TCCCTGATGT CTACCAGGTG 
TCAGTACCCG TCCGCCTGCC AGGCCTGGGC CTGGGTGTGC TGCAGCTGCA GCCAGATCTC 
GATGGGCCCT ACACGCTGCA GTCTTCCGTG CGGGTCTACC TGAACGGCGT GAAACTGTCC 
GTCAGCAGGC AGTCAGCATT CCCTGTCCGT GTTGTGGACT CCGGCGCCAG CGACTTCGCC 
ATCAGCAATC GCTACATGCA GGTCTGGTTC TCCGGCCTTA CTGGGCTTCT CAAGAGCATC 
CGACGGGTGG ATGAGGAGCA GGAGCAGCAG ATGGAACTGG AGTTCCTCGT CTACGGCACC 
CGTACCTCCA AGGACAAGAG TGGCGCCTAC CTCTTCCTGC CTGATAGCGA GGCTAAGCCC 
TACGTCCCTA AGAAACCTCC TGTGCTGCGT GTCACCGAAG GCCCTTTCTT CTCAGAGGTG 
GCTGTGTACT ATGAGCACTT TCACCAAGTG ATTCGACTTT ATAACCTGCC AGGGGTAGAG 
GGGCTGTCTC TGGACATGTC ATTCCAGGTA GATATCAGGG ACTATGTGAA CAAGGAACTG 
GCCCTGCGCA TCCACACAGA CATTGACAGC CAGGGCACTT TCTTCACAGA CCTCAATGGC 
TTTCAGATAC AGCCCCGGCA GTATCTGAAG AAGCTGCCCC TGCAGGCTAA TTTCTACCCT 
ATGCCAGTCA TGGCCTATAT CCAAGATTCC CAGAGGCGCC TCACGCTGCA CACTGCCCAG 
GCTCTGGGTG TCTCCAGCCT CGGTAATGGC CAGCTGGAGG TGATCTTGGA CCGAAGGCTA 
ATGCAGGATG ACAACCGGGG ACTAGGCCAA GGGCTCAAAG ACAACAAGAT CACCTGCAAC 
CGTTTCCGCC TCCTGTTAGA ACGTCGGACC ACAATGAGCC CAGAGGTGCA TCAGGAGCAG 
GAGCGTTCTA CAAGCTACCC GTCCCTCCTC AGCCACCTGA CATCCATGTA CCTCAGCACA 
CCTCCTCTTG TCTTACCCGT GGCCAAGAGG CAGGGCACTA GCCCTGCTCT GCGCTCTTTC 
CACCCTCTGG CTTCTCCATT GCCCTGTGAC TTCCATTTGC TCAATCTGCG CATGCTCCCA 
GCCGAGGACA CCTTGCCCGC AACTGACTCT GCGCTCATCT TACACCGCAA GGGGTTTGAC 
TGTGGCCTTG AAGCCAAGAA CCTGGGCTTC AACTGTACCA CAAGCCAAGG CAAGCTAGCC 
CTGGGGAGCC TCTTCCATGG CCTGGATGTG ACCTTCCTGC AGCCAACCTC CTTGACTTTG 
CTATACCCTC TGGCCTCACC TTCCAACAGC ACTGACATCT CTCTGGAGCC CATGGAGATC 
AGCACCTTCC GCCTGCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540663.1
CDS	3249..4835
Translation	MDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVHWSSATDMVPDVYQVSVPVRLPGLGLGVLQLQPDLDGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFAISNRYMQVWFSGLTGLLKSIRRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLRVTEGPFFSEVAVYYEHFHQVIRLYNLPGVEGLSLDMSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQIQPRQYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNRFRLLLERRTTMSPEVHQEQERSTSYPSLLSHLTSMYLSTPPLVLPVAKRQGTSPALRSFHPLASPLPCDFHLLNLRMLPAEDTLPATDSALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVTFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	XM_006540600.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540600.4

ORF Insert Sequence:

ATGGATGACA GCCGTGTAAG CCATGATGCC CTGCCCGAGC GCACGGTGAT CCGGCTGGAC 
TCCTCACCCA GGTTTGTGGT GGTATTTAAC CCGCTGGAAC AGGAGCGGCT CAGTGTGGTG 
TCCCTGCTGG TCAACTCGCC CCGGGTGCGC GTCCTTTCAG AGGAGGGCCA GCCCCTGTCT 
GTGCAGATCA GTGTGCACTG GAGCTCGGCT ACGGACATGG TCCCTGATGT CTACCAGGTG 
TCAGTACCCG TCCGCCTGCC AGGCCTGGGC CTGGGTGTGC TGCAGCTGCA GCCAGATCTC 
GATGGGCCCT ACACGCTGCA GTCTTCCGTG CGGGTCTACC TGAACGGCGT GAAACTGTCC 
GTCAGCAGGC AGTCAGCATT CCCTGTCCGT GTTGTGGACT CCGGCGCCAG CGACTTCGCC 
ATCAGCAATC GCTACATGCA GGTCTGGTTC TCCGGCCTTA CTGGGCTTCT CAAGAGCATC 
CGACGGGTGG ATGAGGAGCA GGAGCAGCAG ATGGAACTGG AGTTCCTCGT CTACGGCACC 
CGTACCTCCA AGGACAAGAG TGGCGCCTAC CTCTTCCTGC CTGATAGCGA GGCTAAGCCC 
TACGTCCCTA AGAAACCTCC TGTGCTGCGT GTCACCGAAG GCCCTTTCTT CTCAGAGGTG 
GCTGTGTACT ATGAGCACTT TCACCAAGTG ATTCGACTTT ATAACCTGCC AGGGGTAGAG 
GGGCTGTCTC TGGACATGTC ATTCCAGGTA GATATCAGGG ACTATGTGAA CAAGGAACTG 
GCCCTGCGCA TCCACACAGA CATTGACAGC CAGGGCACTT TCTTCACAGA CCTCAATGGC 
TTTCAGATAC AGCCCCGGCA GTATCTGAAG AAGCTGCCCC TGCAGGCTAA TTTCTACCCT 
ATGCCAGTCA TGGCCTATAT CCAAGATTCC CAGAGGCGCC TCACGCTGCA CACTGCCCAG 
GCTCTGGGTG TCTCCAGCCT CGGTAATGGC CAGCTGGAGG TGATCTTGGA CCGAAGGCTA 
ATGCAGGATG ACAACCGGGG ACTAGGCCAA GGGCTCAAAG ACAACAAGAT CACCTGCAAC 
CGTTTCCGCC TCCTGTTAGA ACGTCGGACC ACAATGAGCC CAGAGGTGCA TCAGGAGCAG 
GAGCGTTCTA CAAGCTACCC GTCCCTCCTC AGCCACCTGA CATCCATGTA CCTCAGCACA 
CCTCCTCTTG TCTTACCCGT GGCCAAGAGG CAGGGCACTA GCCCTGCTCT GCGCTCTTTC 
CACCCTCTGG CTTCTCCATT GCCCTGTGAC TTCCATTTGC TCAATCTGCG CATGCTCCCA 
GCCGAGGACA CCTTGCCCGC AACTGACTCT GCGCTCATCT TACACCGCAA GGGGTTTGAC 
TGTGGCCTTG AAGCCAAGAA CCTGGGCTTC AACTGTACCA CAAGCCAAGG CAAGCTAGCC 
CTGGGGAGCC TCTTCCATGG CCTGGATGTG ACCTTCCTGC AGCCAACCTC CTTGACTTTG 
CTATACCCTC TGGCCTCACC TTCCAACAGC ACTGACATCT CTCTGGAGCC CATGGAGATC 
AGCACCTTCC GCCTGCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a2 ( XM_006540600.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540663.1 Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu38661
Clone ID Related Accession (Same CDS sequence)	XM_006540600.4 , XM_006540600.3
Accession Version	XM_006540600.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540600.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA GCCGTGTAAG CCATGATGCC CTGCCCGAGC GCACGGTGAT CCGGCTGGAC 
TCCTCACCCA GGTTTGTGGT GGTATTTAAC CCGCTGGAAC AGGAGCGGCT CAGTGTGGTG 
TCCCTGCTGG TCAACTCGCC CCGGGTGCGC GTCCTTTCAG AGGAGGGCCA GCCCCTGTCT 
GTGCAGATCA GTGTGCACTG GAGCTCGGCT ACGGACATGG TCCCTGATGT CTACCAGGTG 
TCAGTACCCG TCCGCCTGCC AGGCCTGGGC CTGGGTGTGC TGCAGCTGCA GCCAGATCTC 
GATGGGCCCT ACACGCTGCA GTCTTCCGTG CGGGTCTACC TGAACGGCGT GAAACTGTCC 
GTCAGCAGGC AGTCAGCATT CCCTGTCCGT GTTGTGGACT CCGGCGCCAG CGACTTCGCC 
ATCAGCAATC GCTACATGCA GGTCTGGTTC TCCGGCCTTA CTGGGCTTCT CAAGAGCATC 
CGACGGGTGG ATGAGGAGCA GGAGCAGCAG ATGGAACTGG AGTTCCTCGT CTACGGCACC 
CGTACCTCCA AGGACAAGAG TGGCGCCTAC CTCTTCCTGC CTGATAGCGA GGCTAAGCCC 
TACGTCCCTA AGAAACCTCC TGTGCTGCGT GTCACCGAAG GCCCTTTCTT CTCAGAGGTG 
GCTGTGTACT ATGAGCACTT TCACCAAGTG ATTCGACTTT ATAACCTGCC AGGGGTAGAG 
GGGCTGTCTC TGGACATGTC ATTCCAGGTA GATATCAGGG ACTATGTGAA CAAGGAACTG 
GCCCTGCGCA TCCACACAGA CATTGACAGC CAGGGCACTT TCTTCACAGA CCTCAATGGC 
TTTCAGATAC AGCCCCGGCA GTATCTGAAG AAGCTGCCCC TGCAGGCTAA TTTCTACCCT 
ATGCCAGTCA TGGCCTATAT CCAAGATTCC CAGAGGCGCC TCACGCTGCA CACTGCCCAG 
GCTCTGGGTG TCTCCAGCCT CGGTAATGGC CAGCTGGAGG TGATCTTGGA CCGAAGGCTA 
ATGCAGGATG ACAACCGGGG ACTAGGCCAA GGGCTCAAAG ACAACAAGAT CACCTGCAAC 
CGTTTCCGCC TCCTGTTAGA ACGTCGGACC ACAATGAGCC CAGAGGTGCA TCAGGAGCAG 
GAGCGTTCTA CAAGCTACCC GTCCCTCCTC AGCCACCTGA CATCCATGTA CCTCAGCACA 
CCTCCTCTTG TCTTACCCGT GGCCAAGAGG CAGGGCACTA GCCCTGCTCT GCGCTCTTTC 
CACCCTCTGG CTTCTCCATT GCCCTGTGAC TTCCATTTGC TCAATCTGCG CATGCTCCCA 
GCCGAGGACA CCTTGCCCGC AACTGACTCT GCGCTCATCT TACACCGCAA GGGGTTTGAC 
TGTGGCCTTG AAGCCAAGAA CCTGGGCTTC AACTGTACCA CAAGCCAAGG CAAGCTAGCC 
CTGGGGAGCC TCTTCCATGG CCTGGATGTG ACCTTCCTGC AGCCAACCTC CTTGACTTTG 
CTATACCCTC TGGCCTCACC TTCCAACAGC ACTGACATCT CTCTGGAGCC CATGGAGATC 
AGCACCTTCC GCCTGCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540663.1
CDS	232..1818
Translation	MDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVHWSSATDMVPDVYQVSVPVRLPGLGLGVLQLQPDLDGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFAISNRYMQVWFSGLTGLLKSIRRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLRVTEGPFFSEVAVYYEHFHQVIRLYNLPGVEGLSLDMSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQIQPRQYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNRFRLLLERRTTMSPEVHQEQERSTSYPSLLSHLTSMYLSTPPLVLPVAKRQGTSPALRSFHPLASPLPCDFHLLNLRMLPAEDTLPATDSALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVTFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	XM_006540600.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540600.3

ORF Insert Sequence:

ATGGATGACA GCCGTGTAAG CCATGATGCC CTGCCCGAGC GCACGGTGAT CCGGCTGGAC 
TCCTCACCCA GGTTTGTGGT GGTATTTAAC CCGCTGGAAC AGGAGCGGCT CAGTGTGGTG 
TCCCTGCTGG TCAACTCGCC CCGGGTGCGC GTCCTTTCAG AGGAGGGCCA GCCCCTGTCT 
GTGCAGATCA GTGTGCACTG GAGCTCGGCT ACGGACATGG TCCCTGATGT CTACCAGGTG 
TCAGTACCCG TCCGCCTGCC AGGCCTGGGC CTGGGTGTGC TGCAGCTGCA GCCAGATCTC 
GATGGGCCCT ACACGCTGCA GTCTTCCGTG CGGGTCTACC TGAACGGCGT GAAACTGTCC 
GTCAGCAGGC AGTCAGCATT CCCTGTCCGT GTTGTGGACT CCGGCGCCAG CGACTTCGCC 
ATCAGCAATC GCTACATGCA GGTCTGGTTC TCCGGCCTTA CTGGGCTTCT CAAGAGCATC 
CGACGGGTGG ATGAGGAGCA GGAGCAGCAG ATGGAACTGG AGTTCCTCGT CTACGGCACC 
CGTACCTCCA AGGACAAGAG TGGCGCCTAC CTCTTCCTGC CTGATAGCGA GGCTAAGCCC 
TACGTCCCTA AGAAACCTCC TGTGCTGCGT GTCACCGAAG GCCCTTTCTT CTCAGAGGTG 
GCTGTGTACT ATGAGCACTT TCACCAAGTG ATTCGACTTT ATAACCTGCC AGGGGTAGAG 
GGGCTGTCTC TGGACATGTC ATTCCAGGTA GATATCAGGG ACTATGTGAA CAAGGAACTG 
GCCCTGCGCA TCCACACAGA CATTGACAGC CAGGGCACTT TCTTCACAGA CCTCAATGGC 
TTTCAGATAC AGCCCCGGCA GTATCTGAAG AAGCTGCCCC TGCAGGCTAA TTTCTACCCT 
ATGCCAGTCA TGGCCTATAT CCAAGATTCC CAGAGGCGCC TCACGCTGCA CACTGCCCAG 
GCTCTGGGTG TCTCCAGCCT CGGTAATGGC CAGCTGGAGG TGATCTTGGA CCGAAGGCTA 
ATGCAGGATG ACAACCGGGG ACTAGGCCAA GGGCTCAAAG ACAACAAGAT CACCTGCAAC 
CGTTTCCGCC TCCTGTTAGA ACGTCGGACC ACAATGAGCC CAGAGGTGCA TCAGGAGCAG 
GAGCGTTCTA CAAGCTACCC GTCCCTCCTC AGCCACCTGA CATCCATGTA CCTCAGCACA 
CCTCCTCTTG TCTTACCCGT GGCCAAGAGG CAGGGCACTA GCCCTGCTCT GCGCTCTTTC 
CACCCTCTGG CTTCTCCATT GCCCTGTGAC TTCCATTTGC TCAATCTGCG CATGCTCCCA 
GCCGAGGACA CCTTGCCCGC AACTGACTCT GCGCTCATCT TACACCGCAA GGGGTTTGAC 
TGTGGCCTTG AAGCCAAGAA CCTGGGCTTC AACTGTACCA CAAGCCAAGG CAAGCTAGCC 
CTGGGGAGCC TCTTCCATGG CCTGGATGTG ACCTTCCTGC AGCCAACCTC CTTGACTTTG 
CTATACCCTC TGGCCTCACC TTCCAACAGC ACTGACATCT CTCTGGAGCC CATGGAGATC 
AGCACCTTCC GCCTGCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a2 ( NM_172903.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766491.2 Mus musculus mannosidase 2, alpha 2 (Man2a2), mRNA.

Price & Availability↑

CloneID	OMu04272
Clone ID Related Accession (Same CDS sequence)	NM_172903.4 , XM_006540599.3
Accession Version	NM_172903.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	alpha-mannosidase 2x
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ105476.1, AK029913.1, AC136740.10 and BC055006.1. On Mar 26, 2011 this sequence version replaced NM_172903.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK029913.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTGA AAAAGCAGGT GACGGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCTCTGTACC TTATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT CTCTGTGCTA CAGAACCGGA TAGAACAGCT GGAACAACTG 
CTGGAGGAAA ACCATGACAT CATAAGCCGT ATCAAGGACT CTGTACTGGA ACTGACAGCC 
AATGCGGAGG GCCCGCCAGC CCTGCTGCCC TACCATACCG CCAACGGTTC CTGGGCTGTG 
CTCCCGGAGC CCCGGCCCAG CTTCTTCTCC GTCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGACGGG GTCAGAAGCC AGAACTACAG ATGTTAACCG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGTGGCGT GTGGAGGCAA GGCTTTGACA TCTCCTACAG CCCAAATGAC 
TGGGACACTG AAGACCTGCA AGTGTTTGTG GTGCCTCACT CCCACAACGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGACCC CCGGCGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGCGAC AGGCGGATGG GTGATGCCCG ACGAGGCCAA CTCCCATTAC 
TTTGCCTTGG TTGACCAGCT CATCGAGGGG CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACCCCGCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTG CCACTCATAG CCTGGAGTTC ATGTGGAGGC AGATGTGGGA TTCAGACTCC 
AGCACAGACA TCTTTTGCCA CATGATGCCC TTCTACAGCT ACGACGTTCC ACACACCTGT 
GGCCCCGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGCCCTT GGAAGGTGCC ACCCCGGGCT ATTACAGAAG CCAATGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTC 
GTGCCACTTG GTGATGACTT CCGGTATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG AGTTCCATGT GCAGGCCCAG 
TTTGGGACCC TCTCTGAGTA TTTCGATGCC CTGTATAAGA GGACAGGAGT AGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTACGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACCTCC CGGCCTTTCT ACAAGAGCTT GGACCGCGTC 
CTAGAAGCCC ACCTGCGTGG GGCAGAGATT CTATACAGCC TGGCTTTGGC CCATGCCCGC 
CGTTCTGGAC TGGCCGGCCA GTACCCGCTG TCTGATTTTG CTCTTCTGAC GGAAGCTCGG 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACGG GAACCGCCAA GGAGGCAGTA 
GTGGTAGACT ACGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTCAAGCA GGTTATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC CAGGAGACCT ACAGCTTTGA CCCTGGGACA 
CCCTTCCTCC AAATGGATGA CAGCCGTGTA AGCCATGATG CCCTGCCCGA GCGCACGGTG 
ATCCGGCTGG ACTCCTCACC CAGGTTTGTG GTGGTATTTA ACCCGCTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCG CCCCGGGTGC GCGTCCTTTC AGAGGAGGGC 
CAGCCCCTGT CTGTGCAGAT CAGTGTGCAC TGGAGCTCGG CTACGGACAT GGTCCCTGAT 
GTCTACCAGG TGTCAGTACC CGTCCGCCTG CCAGGCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGGCC CTACACGCTG CAGTCTTCCG TGCGGGTCTA CCTGAACGGC 
GTGAAACTGT CCGTCAGCAG GCAGTCAGCA TTCCCTGTCC GTGTTGTGGA CTCCGGCGCC 
AGCGACTTCG CCATCAGCAA TCGCTACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCA TCCGACGGGT GGATGAGGAG CAGGAGCAGC AGATGGAACT GGAGTTCCTC 
GTCTACGGCA CCCGTACCTC CAAGGACAAG AGTGGCGCCT ACCTCTTCCT GCCTGATAGC 
GAGGCTAAGC CCTACGTCCC TAAGAAACCT CCTGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGTGTA CTATGAGCAC TTTCACCAAG TGATTCGACT TTATAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACATG TCATTCCAGG TAGATATCAG GGACTATGTG 
AACAAGGAAC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGAT ACAGCCCCGG CAGTATCTGA AGAAGCTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAT ATCCAAGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCCC AGGCTCTGGG TGTCTCCAGC CTCGGTAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCGTTTCCG CCTCCTGTTA GAACGTCGGA CCACAATGAG CCCAGAGGTG 
CATCAGGAGC AGGAGCGTTC TACAAGCTAC CCGTCCCTCC TCAGCCACCT GACATCCATG 
TACCTCAGCA CACCTCCTCT TGTCTTACCC GTGGCCAAGA GGCAGGGCAC TAGCCCTGCT 
CTGCGCTCTT TCCACCCTCT GGCTTCTCCA TTGCCCTGTG ACTTCCATTT GCTCAATCTG 
CGCATGCTCC CAGCCGAGGA CACCTTGCCC GCAACTGACT CTGCGCTCAT CTTACACCGC 
AAGGGGTTTG ACTGTGGCCT TGAAGCCAAG AACCTGGGCT TCAACTGTAC CACAAGCCAA 
GGCAAGCTAG CCCTGGGGAG CCTCTTCCAT GGCCTGGATG TGACCTTCCT GCAGCCAACC 
TCCTTGACTT TGCTATACCC TCTGGCCTCA CCTTCCAACA GCACTGACAT CTCTCTGGAG 
CCCATGGAGA TCAGCACCTT CCGCCTGCGC TTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766491.2
CDS	494..3952
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHDIISRIKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPQDCQFALGGRGQKPELQMLTVSEDLPFDNVEGGVWRQGFDISYSPNDWDTEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQMWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLALAHARRSGLAGQYPLSDFALLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDQETYSFDPGTPFLQMDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVHWSSATDMVPDVYQVSVPVRLPGLGLGVLQLQPDLDGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFAISNRYMQVWFSGLTGLLKSIRRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLRVTEGPFFSEVAVYYEHFHQVIRLYNLPGVEGLSLDMSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQIQPRQYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNRFRLLLERRTTMSPEVHQEQERSTSYPSLLSHLTSMYLSTPPLVLPVAKRQGTSPALRSFHPLASPLPCDFHLLNLRMLPAEDTLPATDSALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVTFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	NM_172903.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha 2 (Man2a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172903.4

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACGGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCTCTGTACC TTATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT CTCTGTGCTA CAGAACCGGA TAGAACAGCT GGAACAACTG 
CTGGAGGAAA ACCATGACAT CATAAGCCGT ATCAAGGACT CTGTACTGGA ACTGACAGCC 
AATGCGGAGG GCCCGCCAGC CCTGCTGCCC TACCATACCG CCAACGGTTC CTGGGCTGTG 
CTCCCGGAGC CCCGGCCCAG CTTCTTCTCC GTCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGACGGG GTCAGAAGCC AGAACTACAG ATGTTAACCG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGTGGCGT GTGGAGGCAA GGCTTTGACA TCTCCTACAG CCCAAATGAC 
TGGGACACTG AAGACCTGCA AGTGTTTGTG GTGCCTCACT CCCACAACGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGACCC CCGGCGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGCGAC AGGCGGATGG GTGATGCCCG ACGAGGCCAA CTCCCATTAC 
TTTGCCTTGG TTGACCAGCT CATCGAGGGG CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACCCCGCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTG CCACTCATAG CCTGGAGTTC ATGTGGAGGC AGATGTGGGA TTCAGACTCC 
AGCACAGACA TCTTTTGCCA CATGATGCCC TTCTACAGCT ACGACGTTCC ACACACCTGT 
GGCCCCGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGCCCTT GGAAGGTGCC ACCCCGGGCT ATTACAGAAG CCAATGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTC 
GTGCCACTTG GTGATGACTT CCGGTATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG AGTTCCATGT GCAGGCCCAG 
TTTGGGACCC TCTCTGAGTA TTTCGATGCC CTGTATAAGA GGACAGGAGT AGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTACGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACCTCC CGGCCTTTCT ACAAGAGCTT GGACCGCGTC 
CTAGAAGCCC ACCTGCGTGG GGCAGAGATT CTATACAGCC TGGCTTTGGC CCATGCCCGC 
CGTTCTGGAC TGGCCGGCCA GTACCCGCTG TCTGATTTTG CTCTTCTGAC GGAAGCTCGG 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACGG GAACCGCCAA GGAGGCAGTA 
GTGGTAGACT ACGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTCAAGCA GGTTATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC CAGGAGACCT ACAGCTTTGA CCCTGGGACA 
CCCTTCCTCC AAATGGATGA CAGCCGTGTA AGCCATGATG CCCTGCCCGA GCGCACGGTG 
ATCCGGCTGG ACTCCTCACC CAGGTTTGTG GTGGTATTTA ACCCGCTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCG CCCCGGGTGC GCGTCCTTTC AGAGGAGGGC 
CAGCCCCTGT CTGTGCAGAT CAGTGTGCAC TGGAGCTCGG CTACGGACAT GGTCCCTGAT 
GTCTACCAGG TGTCAGTACC CGTCCGCCTG CCAGGCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGGCC CTACACGCTG CAGTCTTCCG TGCGGGTCTA CCTGAACGGC 
GTGAAACTGT CCGTCAGCAG GCAGTCAGCA TTCCCTGTCC GTGTTGTGGA CTCCGGCGCC 
AGCGACTTCG CCATCAGCAA TCGCTACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCA TCCGACGGGT GGATGAGGAG CAGGAGCAGC AGATGGAACT GGAGTTCCTC 
GTCTACGGCA CCCGTACCTC CAAGGACAAG AGTGGCGCCT ACCTCTTCCT GCCTGATAGC 
GAGGCTAAGC CCTACGTCCC TAAGAAACCT CCTGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGTGTA CTATGAGCAC TTTCACCAAG TGATTCGACT TTATAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACATG TCATTCCAGG TAGATATCAG GGACTATGTG 
AACAAGGAAC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGAT ACAGCCCCGG CAGTATCTGA AGAAGCTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAT ATCCAAGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCCC AGGCTCTGGG TGTCTCCAGC CTCGGTAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCGTTTCCG CCTCCTGTTA GAACGTCGGA CCACAATGAG CCCAGAGGTG 
CATCAGGAGC AGGAGCGTTC TACAAGCTAC CCGTCCCTCC TCAGCCACCT GACATCCATG 
TACCTCAGCA CACCTCCTCT TGTCTTACCC GTGGCCAAGA GGCAGGGCAC TAGCCCTGCT 
CTGCGCTCTT TCCACCCTCT GGCTTCTCCA TTGCCCTGTG ACTTCCATTT GCTCAATCTG 
CGCATGCTCC CAGCCGAGGA CACCTTGCCC GCAACTGACT CTGCGCTCAT CTTACACCGC 
AAGGGGTTTG ACTGTGGCCT TGAAGCCAAG AACCTGGGCT TCAACTGTAC CACAAGCCAA 
GGCAAGCTAG CCCTGGGGAG CCTCTTCCAT GGCCTGGATG TGACCTTCCT GCAGCCAACC 
TCCTTGACTT TGCTATACCC TCTGGCCTCA CCTTCCAACA GCACTGACAT CTCTCTGGAG 
CCCATGGAGA TCAGCACCTT CCGCCTGCGC TTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a2 ( XM_006540599.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540662.1 Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X1, mRNA.

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CloneID	OMu04272
Clone ID Related Accession (Same CDS sequence)	NM_172903.4 , XM_006540599.3
Accession Version	XM_006540599.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACGGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCTCTGTACC TTATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT CTCTGTGCTA CAGAACCGGA TAGAACAGCT GGAACAACTG 
CTGGAGGAAA ACCATGACAT CATAAGCCGT ATCAAGGACT CTGTACTGGA ACTGACAGCC 
AATGCGGAGG GCCCGCCAGC CCTGCTGCCC TACCATACCG CCAACGGTTC CTGGGCTGTG 
CTCCCGGAGC CCCGGCCCAG CTTCTTCTCC GTCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGACGGG GTCAGAAGCC AGAACTACAG ATGTTAACCG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGTGGCGT GTGGAGGCAA GGCTTTGACA TCTCCTACAG CCCAAATGAC 
TGGGACACTG AAGACCTGCA AGTGTTTGTG GTGCCTCACT CCCACAACGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGACCC CCGGCGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGCGAC AGGCGGATGG GTGATGCCCG ACGAGGCCAA CTCCCATTAC 
TTTGCCTTGG TTGACCAGCT CATCGAGGGG CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACCCCGCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTG CCACTCATAG CCTGGAGTTC ATGTGGAGGC AGATGTGGGA TTCAGACTCC 
AGCACAGACA TCTTTTGCCA CATGATGCCC TTCTACAGCT ACGACGTTCC ACACACCTGT 
GGCCCCGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGCCCTT GGAAGGTGCC ACCCCGGGCT ATTACAGAAG CCAATGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTC 
GTGCCACTTG GTGATGACTT CCGGTATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG AGTTCCATGT GCAGGCCCAG 
TTTGGGACCC TCTCTGAGTA TTTCGATGCC CTGTATAAGA GGACAGGAGT AGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTACGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACCTCC CGGCCTTTCT ACAAGAGCTT GGACCGCGTC 
CTAGAAGCCC ACCTGCGTGG GGCAGAGATT CTATACAGCC TGGCTTTGGC CCATGCCCGC 
CGTTCTGGAC TGGCCGGCCA GTACCCGCTG TCTGATTTTG CTCTTCTGAC GGAAGCTCGG 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACGG GAACCGCCAA GGAGGCAGTA 
GTGGTAGACT ACGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTCAAGCA GGTTATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC CAGGAGACCT ACAGCTTTGA CCCTGGGACA 
CCCTTCCTCC AAATGGATGA CAGCCGTGTA AGCCATGATG CCCTGCCCGA GCGCACGGTG 
ATCCGGCTGG ACTCCTCACC CAGGTTTGTG GTGGTATTTA ACCCGCTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCG CCCCGGGTGC GCGTCCTTTC AGAGGAGGGC 
CAGCCCCTGT CTGTGCAGAT CAGTGTGCAC TGGAGCTCGG CTACGGACAT GGTCCCTGAT 
GTCTACCAGG TGTCAGTACC CGTCCGCCTG CCAGGCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGGCC CTACACGCTG CAGTCTTCCG TGCGGGTCTA CCTGAACGGC 
GTGAAACTGT CCGTCAGCAG GCAGTCAGCA TTCCCTGTCC GTGTTGTGGA CTCCGGCGCC 
AGCGACTTCG CCATCAGCAA TCGCTACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCA TCCGACGGGT GGATGAGGAG CAGGAGCAGC AGATGGAACT GGAGTTCCTC 
GTCTACGGCA CCCGTACCTC CAAGGACAAG AGTGGCGCCT ACCTCTTCCT GCCTGATAGC 
GAGGCTAAGC CCTACGTCCC TAAGAAACCT CCTGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGTGTA CTATGAGCAC TTTCACCAAG TGATTCGACT TTATAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACATG TCATTCCAGG TAGATATCAG GGACTATGTG 
AACAAGGAAC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGAT ACAGCCCCGG CAGTATCTGA AGAAGCTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAT ATCCAAGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCCC AGGCTCTGGG TGTCTCCAGC CTCGGTAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCGTTTCCG CCTCCTGTTA GAACGTCGGA CCACAATGAG CCCAGAGGTG 
CATCAGGAGC AGGAGCGTTC TACAAGCTAC CCGTCCCTCC TCAGCCACCT GACATCCATG 
TACCTCAGCA CACCTCCTCT TGTCTTACCC GTGGCCAAGA GGCAGGGCAC TAGCCCTGCT 
CTGCGCTCTT TCCACCCTCT GGCTTCTCCA TTGCCCTGTG ACTTCCATTT GCTCAATCTG 
CGCATGCTCC CAGCCGAGGA CACCTTGCCC GCAACTGACT CTGCGCTCAT CTTACACCGC 
AAGGGGTTTG ACTGTGGCCT TGAAGCCAAG AACCTGGGCT TCAACTGTAC CACAAGCCAA 
GGCAAGCTAG CCCTGGGGAG CCTCTTCCAT GGCCTGGATG TGACCTTCCT GCAGCCAACC 
TCCTTGACTT TGCTATACCC TCTGGCCTCA CCTTCCAACA GCACTGACAT CTCTCTGGAG 
CCCATGGAGA TCAGCACCTT CCGCCTGCGC TTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540662.1
CDS	517..3975
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHDIISRIKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPQDCQFALGGRGQKPELQMLTVSEDLPFDNVEGGVWRQGFDISYSPNDWDTEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQMWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLALAHARRSGLAGQYPLSDFALLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDQETYSFDPGTPFLQMDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVHWSSATDMVPDVYQVSVPVRLPGLGLGVLQLQPDLDGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFAISNRYMQVWFSGLTGLLKSIRRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLRVTEGPFFSEVAVYYEHFHQVIRLYNLPGVEGLSLDMSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQIQPRQYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNRFRLLLERRTTMSPEVHQEQERSTSYPSLLSHLTSMYLSTPPLVLPVAKRQGTSPALRSFHPLASPLPCDFHLLNLRMLPAEDTLPATDSALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVTFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	XM_006540599.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha 2 (Man2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540599.3

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACGGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCTCTGTACC TTATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT CTCTGTGCTA CAGAACCGGA TAGAACAGCT GGAACAACTG 
CTGGAGGAAA ACCATGACAT CATAAGCCGT ATCAAGGACT CTGTACTGGA ACTGACAGCC 
AATGCGGAGG GCCCGCCAGC CCTGCTGCCC TACCATACCG CCAACGGTTC CTGGGCTGTG 
CTCCCGGAGC CCCGGCCCAG CTTCTTCTCC GTCTCCCCCC AGGACTGCCA GTTTGCTTTG 
GGGGGACGGG GTCAGAAGCC AGAACTACAG ATGTTAACCG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGTGGCGT GTGGAGGCAA GGCTTTGACA TCTCCTACAG CCCAAATGAC 
TGGGACACTG AAGACCTGCA AGTGTTTGTG GTGCCTCACT CCCACAACGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAGACCCAGC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGACCC CCGGCGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AGATTGCGAC AGGCGGATGG GTGATGCCCG ACGAGGCCAA CTCCCATTAC 
TTTGCCTTGG TTGACCAGCT CATCGAGGGG CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACCCCGCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG 
CACTTTGCTG CCACTCATAG CCTGGAGTTC ATGTGGAGGC AGATGTGGGA TTCAGACTCC 
AGCACAGACA TCTTTTGCCA CATGATGCCC TTCTACAGCT ACGACGTTCC ACACACCTGT 
GGCCCCGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGCCCTT GGAAGGTGCC ACCCCGGGCT ATTACAGAAG CCAATGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TCCGAAGCAA CGTCCTCCTC 
GTGCCACTTG GTGATGACTT CCGGTATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG AGTTCCATGT GCAGGCCCAG 
TTTGGGACCC TCTCTGAGTA TTTCGATGCC CTGTATAAGA GGACAGGAGT AGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTACGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACCTCC CGGCCTTTCT ACAAGAGCTT GGACCGCGTC 
CTAGAAGCCC ACCTGCGTGG GGCAGAGATT CTATACAGCC TGGCTTTGGC CCATGCCCGC 
CGTTCTGGAC TGGCCGGCCA GTACCCGCTG TCTGATTTTG CTCTTCTGAC GGAAGCTCGG 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACGG GAACCGCCAA GGAGGCAGTA 
GTGGTAGACT ACGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTCAAGCA GGTTATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC CAGGAGACCT ACAGCTTTGA CCCTGGGACA 
CCCTTCCTCC AAATGGATGA CAGCCGTGTA AGCCATGATG CCCTGCCCGA GCGCACGGTG 
ATCCGGCTGG ACTCCTCACC CAGGTTTGTG GTGGTATTTA ACCCGCTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCG CCCCGGGTGC GCGTCCTTTC AGAGGAGGGC 
CAGCCCCTGT CTGTGCAGAT CAGTGTGCAC TGGAGCTCGG CTACGGACAT GGTCCCTGAT 
GTCTACCAGG TGTCAGTACC CGTCCGCCTG CCAGGCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGGCC CTACACGCTG CAGTCTTCCG TGCGGGTCTA CCTGAACGGC 
GTGAAACTGT CCGTCAGCAG GCAGTCAGCA TTCCCTGTCC GTGTTGTGGA CTCCGGCGCC 
AGCGACTTCG CCATCAGCAA TCGCTACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCA TCCGACGGGT GGATGAGGAG CAGGAGCAGC AGATGGAACT GGAGTTCCTC 
GTCTACGGCA CCCGTACCTC CAAGGACAAG AGTGGCGCCT ACCTCTTCCT GCCTGATAGC 
GAGGCTAAGC CCTACGTCCC TAAGAAACCT CCTGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGTGTA CTATGAGCAC TTTCACCAAG TGATTCGACT TTATAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACATG TCATTCCAGG TAGATATCAG GGACTATGTG 
AACAAGGAAC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGAT ACAGCCCCGG CAGTATCTGA AGAAGCTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAT ATCCAAGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCCC AGGCTCTGGG TGTCTCCAGC CTCGGTAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCGTTTCCG CCTCCTGTTA GAACGTCGGA CCACAATGAG CCCAGAGGTG 
CATCAGGAGC AGGAGCGTTC TACAAGCTAC CCGTCCCTCC TCAGCCACCT GACATCCATG 
TACCTCAGCA CACCTCCTCT TGTCTTACCC GTGGCCAAGA GGCAGGGCAC TAGCCCTGCT 
CTGCGCTCTT TCCACCCTCT GGCTTCTCCA TTGCCCTGTG ACTTCCATTT GCTCAATCTG 
CGCATGCTCC CAGCCGAGGA CACCTTGCCC GCAACTGACT CTGCGCTCAT CTTACACCGC 
AAGGGGTTTG ACTGTGGCCT TGAAGCCAAG AACCTGGGCT TCAACTGTAC CACAAGCCAA 
GGCAAGCTAG CCCTGGGGAG CCTCTTCCAT GGCCTGGATG TGACCTTCCT GCAGCCAACC 
TCCTTGACTT TGCTATACCC TCTGGCCTCA CCTTCCAACA GCACTGACAT CTCTCTGGAG 
CCCATGGAGA TCAGCACCTT CCGCCTGCGC TTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

N-Glycan structure analysis using lectins and an alpha-mannosidase activity assay.
Methods in enzymology416304-14(2006)
Akama TO,Fukuda MN

A genetic approach to Mammalian glycan function.
Annual review of biochemistry72643-91(2003)
Lowe JB,Marth JD

In vivo role of alpha-mannosidase IIx: ineffective spermatogenesis resulting from targeted disruption of the Man2a2 in the mouse.
Biochimica et biophysica acta1573(3)382-7(2002 Dec)
Fukuda MN,Akama TO

Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A spermatogonia.
Biochemical and biophysical research communications241(2)439-45(1997 Dec)
Hiramoto S,Tamba M,Kiuchi S,Jin YZ,Bannai S,Sugita Y,Dacheux F,Dacheux JL,Yoshida M,Okamura N

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Man2a2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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