Sptbn5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptbn5
Entrez Gene ID	640524
Full Name	spectrin beta, non-erythrocytic 5
Synonyms	EG640524,Gm354,Spnb5
General protein information	Preferred Names spectrin beta, non-erythrocytic 5 Names spectrin beta chain, brain 4 spectrin beta chain, non-erythrocytic 5 spectrin beta 4 spectrin beta 5 spectrin beta non-erythrocytic 5 (SPTBN5) orthologue
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500537.2	XP_006500600.1	spectrin beta chain, non-erythrocytic 5
NM_001370938.1	NP_001357867.1	spectrin beta chain, non-erythrocytic 5
XM_030251931.1	XP_030107791.1	spectrin beta chain, non-erythrocytic 5 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sptbn5	XP_006500600.1
Gallus gallus (chicken)	SPTBN5	XP_421149.4
Drosophila melanogaster (fruit fly)	kst	NP_995988.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004440	XP_313728.5
Bos taurus (cattle)	SPTBN5	XP_003586608.2
Rattus norvegicus (Norway rat)	Sptbn5	XP_006234865.1
Danio rerio (zebrafish)	LOC569585	XP_698075.4
Caenorhabditis elegans (roundworm)	sma-1	NP_001256383.1
Xenopus tropicalis (tropical clawed frog)	sptbn5	XP_004917485.1
Homo sapiens (human)	SPTBN5	NP_057726.4
Pan troglodytes (chimpanzee)	SPTBN5	XP_001142930.3

Gene Ontology
Bibliography

Related articles in PubMed

Spectrin βV adaptive mutations and changes in subcellular location correlate with emergence of hair cell electromotility in mammalians.
Cortese M, Papal S, Pisciottano F, Elgoyhen AB, Hardelin JP, Petit C, Franchini LF, El-Amraoui A
Proceedings of the National Academy of Sciences of the United States of America114(8)2054-2059(2017 02)

The giant spectrin βV couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route.
Papal S, Cortese M, Legendre K, Sorusch N, Dragavon J, Sahly I, Shorte S, Wolfrum U, Petit C, El-Amraoui A
Human molecular genetics22(18)3773-88(2013 Sep)

alphaII-betaV spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells.
Legendre K, Safieddine S, Küssel-Andermann P, Petit C, El-Amraoui A
Journal of cell science121(Pt 20)3347-56(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sptbn5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptbn5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84877	NM_001370938.1 Latest version!	Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu31110	XM_006500537.2 Latest version!	Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87964	XM_030251931.1 Latest version!	Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptbn5 ( NM_001370938.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357867.1 Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), mRNA.

Price & Availability↑

CloneID	OMu84877
Clone ID Related Accession (Same CDS sequence)	NM_001370938.1
Accession Version	NM_001370938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565280000000
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833774.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01164131 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGACTCTG AGTATGAGAT AGGCCATGTC CGCAAGCTCC AGGCTCAGCA TACACACATG 
CAGGAGAAGA CCTTCACCAA CTGGATCAAT AACATCTTCC GACTAGGACG GGTGGGCATC 
AGGATCCAGA ACCTGTACAC AGAGTTTGCT GACGGTGCCC ACCTACTGCG GCTATTGGAG 
CTTATCTCTG GGGAGGCCCT ACCAGCGCCC AGTCCGGGCC GCCTGCGCGT GCACTTCCTG 
GAGAACAACA GCCACGCCCT GGCCTTCCTC AGGGCCAAGG TGCCCATTCC CTTCATCGGG 
CCAGAGAACA TTGTGGATGG GGACCAGTCA CTCATCCTGG GACTCCTCTG GGTCATCATT 
CTGCGTTTCC AGATTTCCCA CATCTCCTTG GACAGGGAGG AGTTTGGGGC CAGTGCGGCT 
TTGCTGTCTG CCAAGGAAGC CCTGCTGGTC TGGTGTCAGC GGAAGACAGC CGGCTACACC 
AATGTGGACA TCACTGACTT CTCCCGAAGC TGGAGTGACG GGCTCGGCTT TAATGCCCTG 
CTCCACGCTC ACAGGCCGGA CCTGCTGGAT TATGGCTCGC TGAGTCCAGA CCGCCCTCTG 
TATAACCTCT CCTTTGCCTT CCGAGTGGCT GAGCAGCAGC TTGGCATTGC CCAGCTGCTC 
GACCCTGAGG ATGTGGCAGC CCTGCATCCA GATGAGTGCT CCATCATGAC CTACCTGTCT 
CAGTACTACC ACTACTTCTC CCGTCTGCAG CGGGGGCACA CGGCCCAGAG GAGACTGGCT 
AAGATCCTGC TGCAGCTGCA GGAGACGGAG GTATTGCAGG CACAATACAA GCAACTGGTG 
GCTGACCTGC TCTGCTGGAT TGAGGAGAAG AAGATGCAGC TAGAAGCTCG GGACTTCCCA 
GACTCACTGC CTGCCATGCG CCAGCTACTG GCAGCCTTTG CCTCCTTCCG TGCCCGGGAA 
AAGCCACCTC GGTGGCAGCA GCGAGGAGCC ACAGAGGCCC TGCTCTTCCA GCTGCAGACA 
ACACTCCGAG CCCAAAACCG AAGGCCATTC CTACCTCGAG AGGGCCTGGG CCCTGCAGAG 
CTGGCCCAGC GCTGGGCAGA ACTGGAGAGG GCAGAGGCCT GCAGGAGCCA GGCCATGCAG 
CAAAGGCTGC TACAGCTGGA GCGACTGGAC ACTCTGGCCC GCCGCTTTCA GTGCAAGGCA 
TCCCACCGGG AAAGCTTTTT AAATGACGTA GAGCAGATGT TAGACCAGGC CAGAGCCTCC 
CTCACCGACC CAGCCACAGT GGAGGCAGCC ACTCAGAGGC TGAGCGTTCT GGAGGCTGCC 
ATCCTGCCCC AGGAAGGGCG CTTCCAGGCC TTGGGTGAGA TGGCAGACAT CCTCCGGCAG 
GAAGAGTATC ACAGCTGGGC GGATATGGCC CGCAGGCAGA AGGAGATCAC GGGGCGCTGG 
CGGAGGCTCC TACGGTGTCT ACAGGAGGAG AGGAAGCGCA TGGAAGACTC AAAGGCTGTG 
CTGAGTCTGC TGCAGGGGGT GGAGGCTGTC ACTCACCAGC TTGGGGAGCT GCAGGTGTTG 
GCCAGCTCCA CAGTCTATGG GCAACAGCTG GCAGAAATAG TGTCGCTGCT GCAGAGCCAT 
GACCTGCTGG AGGCCCAGGT GTCAGCCCAC AGGACCCATG TGACTCATCT TGTCCACCAG 
ACCACCCAGC TAGACTCCCA GGGTACCAGT GTGGAGGTGC TGCAGGCTAA AGCCCTAGCA 
CTGGCCGAGC TCCACCACAG CCTGGTGTCT CTTGTCAGAG CCCGACGTAC CCTGCTGGAG 
CAGACCCTCC AGCGAGCACA GTTTCTGCGC AGCTGTGAGG AGGAGGAGGC CTGGTTGCAA 
GAGCACAGAC AGCTAATGGA GACCGCGGTC CTGGACCGGG ACCTCACTCA GATCGCTACT 
GCTCTGCAGA AACACAAGGC TCTGGAAACA GAACTCCATC GCCACCAGGC AGTGTGTGTA 
GATCTCATGC AGAGAGGACG CAACTTCAGT GTCAGAGAGC CCCTGACACA GCCAGATCCA 
CTGGAAAGGG CAGAAGCAGT GCAGGGAACC TGGCAGCTGC TCTGGGCTGG AGCCGCAAGG 
CGGCGCGCCC GGCTGCAAAC AGCACTGCTA ATTGGGCAGT ACTTTTCTGA CTCGGCCGAA 
GCAGCTTCCT GGCTTTTCCA GCGGCAGAAA CAACTAGAGA GCGCATCCTG CGGGAAGGAC 
CAGGCAGACG CCGAGGCCCT GTTGCTGCAA CACCTGCGCC TGGAGCAAGA CGTGCGCGCC 
TTCGCGGCGG AGCTGCGCGA GCTAGAGGAG CAGGCGCGGG CTGCCGCAGC CCTAGTGTCA 
CACATGGTGC ACACGGGGAC ATGGAAAGGA TCCAGACTTG TCTCTCCTAC ACCAAATGAG 
GAACCTCCAG AAGACTGGAA AAGGCTTGGC AGAGACACCA TTGGAGGACC CCAGGGGGAG 
ACTCAAGTTT TAGCAGCACC AGGCAGCCAT TATTCACATC AGCAGATAGC CTTCTTCTCC 
AGAGAAGAGG CTCCAAATAC CTGGAAGCTT GAAGAGTTGG GAGACAGTCA GAGCAGGATG 
GCCATCATCC ACAGCATCGA GAAGAAACCC CAGGTGCTAT CTGCCCTGGG CTTTCTAGGA 
GAAGGCCCGA GGACAGTTCT GCAGGCTGAG AATGACGTTC ACTTCAACTC CAGCACCATA 
CTCCAGGCTC AGGCTGACGT GAGCCAGACC TATGAGAAGC TTAGGGCCCT GGCAAAGCTT 
CACAGGACCC GACTGGAGGA GTCAATCGCC CTGTTTAGCT TTTATAGCTC CTGTAGGGAG 
CTTCAGTCCT GGCTGGAGAA GCAGACCGCA CTGTTCCAAA CATTGCAGCC CCAGGGACAC 
AACTTAGAGG TCATACAACT CAAATATGAG AATGTTCTCA TGACCTTGGC CACTGGAAAA 
GGCCACTGGG CAGAGGCCAT CAACACTGCT GAACAACTGA AGCAGAGATG TCCAGGACAC 
ATCTCGAAAA TCCAACAACA ACAGGAGGAC CTGAAGCAAA GGTGGCAGCA GCTGGAAGCC 
CTGAAGGAGG AGAAACTCCT GCAGCTGACA CGTGGCATGG AGGTGTGCAG TCTTTTACAG 
GAGTCTGAAC CCATACAAGC CCAACTGCTA AATGTGATAA GCAGGCTGGA GACTCTGGGA 
ACAAGAAGCT CTGGGGACAG CCATCATACC CTACAGCAGA CCCAGCAGAA GGTCCTGGTA 
TTGGAGAAGA AGATCTCCTA CCTCCAAAGA GCAACCATAG AGGTGATGGA GTCAGGCTCC 
CCAGAGAGCA GGCTGCTTTG GGAGCAGGTC CTGATGCTGC AGTCACTGCT AAAGCAAGCG 
CAAGGGCAGG TGGCCCGGCA GATCCAGGTC CAGACTGTGG CTCGGGTTCA GCGAGGCATC 
CTCCAGGAGA GCCAGAAGCT GCTGCTGTGG GCAGAGGACA TCCAGGCTCA GCTGTGCAGC 
AAAGAGGAGC GGCAGGTTGA GGCTTCAGCC CTGCGGCCGC TGAGGAGGCA TGGAACCCTA 
CAGGAGGAGA CCTGCCTGTG GGAAGAGAGG CTGCAGCAGC TGGAGGCTCA GGGTCAGCCG 
GTGGCAGTCT CAGACTCTCC ACAGTCCCAG GAGGTGGCCA GCGCTCTAAG GCTCCTAGGC 
CAGCACAGCC AGCAGCTGAA GGCTCTGTGG GAGCAGAGAC AGCAGAAGTT TTGGGAGGGG 
CTGGAGCTGC AGGGGTTTGG CCAAGAAGTA GATGATTTCA TGGCCACCTG TGCCAGCCAT 
GAGGCCTTAC TGCAGCTGGA CAACCTTGGG GAGGACATAA GGGAGGCCCA GAGCCTGCTG 
CAGCAGCACC AGGGTTTGGG GTGGCTCCGC AGCACCCTGG GATCACGGGC AGAGGCCCTC 
CGTGCACGCG GTGAGAAGCT GCTTCTGAGC CATCCAGCTG CCGTGAGCAA GATCAGAGAG 
CTGCTGCACA GTGCCCAGGC CCAGTGGACC AGGGTCCAAG AGAGGAGTGA GCAGAGAAGG 
GTCCAGCTGC TGGCTTCCCT CCAATTGCAG GAATGGAAGC AGGCTGAAGA AGGCCTAATG 
CTGTGGATGG AGGAGAAGTG GCCCAGGGTG GCTGATGAGC GCTCCCAAGC AGGAAGCAAC 
ATTCTGCAGA AACTCAAGTG GCGCAAAATA ACTAAGAGTG AGCTATTAGC CTCTCGGAGG 
TACATGGAAG AGCTACAGCA GGCTGGGAAA GAACTGTTGC ATAGCAACCC ATATGCACAG 
GAGGACATAC AGGACAGACT TCAAAGCCTT AATCACAAAT GGGAAGAGTT GAACCACAAG 
ATGGAAGATC GAGGGGATAG GCTGCCTCAG ACCAGACAGC AGGACCAGCT CCTAGAACTA 
ATACAGGATG TCCAGGAGAC AATGGAGCAT CTGGAGGGAG CCCTGCAGAG CACAGAAACC 
GGGCAGGACC TGTGCTCCAG CCGGAGGCTG CAGAGACAGC ATTGCAAGCT AGAGGACAAG 
AGCCAGGCAC TGGCCAGCAA GATGGATGCC CTCATCTCTC AGACCCACAA TGCATTCACT 
TCCTGGACCA TCCTGGAGGA GAGCCAGAAG TGTCACCAGA GGTTCAAGTC CCTGCAGAGC 
AAGCTAGCCA CCCAACACCA GCAACTGCAG GCCTCAGTTG AGCTGTATGA ATTTAATCTC 
CTTAGCAACC TGGAGCTCAC ATGGGCAGCT GAGCACATGC CCAATGCCGC TCTCAACTGC 
CCTGCCCAGT GCTGGCATGA TGCCCACAGC CTTCAACGAA AGCACAAGAT GCTCCAGGCT 
GAGGTGAAAG GTCATGTAAG GCACATGTAC AGGGTGCTAA GTTCTGGTCA GCGTCTAGCA 
GCCTCTGGGC ACCCTCGAGC TCAGCATATC GTGGAACAGT GCCAGAAGCT AGAAAGCCAC 
TGGGCAGGAC TGGAGCAGGC GTGTGAAGAA CGTGCACACT GCCTGCAACA GGCTGTCACT 
GTCCAGCAGT ACTTTCTGAA CGTGTCAGAG ATGGAGACCT GGGTGGAGGA AAAGCGGCCT 
CTGGCAAGCA GTCAGGACTA CGGCAGTAAT GAGGAAGCTA CCAGTGGGCT CATTAGGAAA 
CACCAGATGC TCCAGCAGGA GGTAGCTCTT TATTGGAGCT CCATGGAAGA TCTCGAGCAG 
AGGTTCCAGA CCCTTGCTGA ATTTGAGGCC CCTGAGCGGT TGGGTGTGGT ACGGGAGAAG 
TTGCAGGCAC TACGGAAGCT GGCAGACGAA CGGGGGCAGG AGCTGGAGGG GACCCTGAGA 
CTACATGAGT TCATGAGAGA AGCTGAGGAC CTGCAGAGCT GGCTGTCCAG CAGGAAGCAG 
GTGGCCAGAG GAGGGGACAC CTTTGGAGAG GACCATGAGC ACGTGCTGCA ACTCTGCACT 
GAGTTTACAA AGTTTCAGTA CCAGGTTGAA ACTGGTGCCC AGCGAGTGGA AACCTGTCGA 
CTGCTGGCAG AGAGCCTACA AGAGCGTGGG CACAGTGCTG CCCCCAAGGC TCACCAGAGG 
CAGCAGGATA TACAGGCTTC CTGGTCTGAA CTGTGCCAGT TGACCCAGGC CCGAAGCCGT 
CTGCTCAATG ACGCCGAAAT CACTCTTAGA GTCCACGGAG ACCTTCTGGA AGTCCTTACC 
CAGATTCAGG AGAAAGCCAC AAGCCTCCCT AACGATGTGG CCCAGGACCT GTGTGGGGTA 
GAGAACCAGC TCCAGAGACA TGAGAGGCTG GAACGCGAGT TGGCAGGCAT GGAGCAGCAG 
GTGCAGGAAC TGATGAAGGC TGGAGGCAGA GTGCAGGAGC TGTGTCCAGG AACTCAGGCC 
CTTGCCGCTG TCCAGCAAAA GCAGCAAGCT GTGACCCAAG CTTGGGAGGC CCTCCAGCTG 
CGCATGGAGC AGCGCAAGGC TCAATTGGAA CGAGGATATC TCCTGGTCCG GTTCCACACA 
GCGGTGAGGG ACTATACCTC CTGGGCGGCC AGCGTGCATC AGGAGCTACA GATGGAGGAG 
GCCTCCTGGG AACCCCACAG CCTTTTGCTC AGGCTCAGAG CCCATCAATG GCTGTGGGCA 
GAACTGAAAG CCAAGGAGGA GCTGCAGCAA CGGGCCACTA AGATGGGCCA GCAAGCTCTG 
CTGGCAGCAG GAACACCCGC TAAGGTCCAG GATGGGCTTC GAACCCTACA GGAGGAACGC 
GACCAAGTGT TCCAGGCCTG GGCACTCAAG CAAGAGAAGC TGCAGGCCAT GCTTCAAGAA 
CAGCACCTCC TCAGGCAATG CGGCCACCTA GTGGAGGTCC TCACAGCCCA GGAGGCATTC 
CTAAAAGCCA GTGGCCTGGG GAGCTCAGTG GAAGAAGTGG AGCAGTTGAT CCGAAAGCAC 
GTGATCTTCC AGAAAGTGCT AGCTCTCCAG GACAAGAAGG AGTCGGCTCT GAATGAGCAG 
CTAAAGACGA TTTCAAGCCC TAAGGGGCAG AACCTGTTTT GCCACATGTT GGAGCACCGG 
GCTCAAGTGA AGGAACTGGC AGAGAGCCGG GGCCAGGCTC TGCACACCTC CCTGATGATA 
GGCAACTTTG TCAGGGCAGC AACCCAGGCT GAGGACTGGA TCCAGCAGCG GGTCCAGCAA 
CTGAGAGCCT GGAGCCCTCT TGGGAATCTA AAGGATTATC TGAAGCACCT GAGGAAACAC 
CAGGCCTTCA GGGCTGAGGT CCAGGCTCAG GAGCAGATCT TGACCTCTGT TGCCAAGCAA 
GGCGAAGAGC TCCTCAGTCA GAGCCACCCT CAAGCTGGAG AGGTCTCCCA GAGGCTGGAG 
GCCCTGCGGG ACCTCTGGGA GAAGCTGAGG CAGGCAGTGA CCCTGCAGGG CCAGGCCCTA 
GAGAACAGAT ACAACTTCCA GGAGTTCCTG CAGAGAGTGG ACCTAGCAGA GACCTGGATC 
CAGGAGAAGG AGAGGATGGT GAACAGCTGT GACATTGGCC TGAACCTTGA GCACTGCCTG 
CAGCTCTGCA GACAAGTCCG CAGGTTACAG GTCACAGTGG ATGATGCCCA CATCAAGGGA 
ATCAAAAACT TGTCACTGCA GCTCAAGAAC CAGGGCCCTG AGGAATCCGA GACCATCTGC 
CAGCGGCAAA ACCAGCTCAA CAATAGGTGG AAGACTTTCC ATGGCAACCT GCTCCTCTAT 
CAGCAGCGGC TTGAAGCAGC CCTGGAGATA CATAGATTGT CCAGGGAACT GGATGACGTT 
ATTGAGCGGC TCAGGGAGAA GGAATCCCTG ATCTGGGCCC CAGAGGGCAC TGAAGATTTG 
GAGAATGTGC AGAGGCTATC ATGGAGACAG AAAGTGCTGC AGCAGGAGAT GGGCCTCATC 
CAGACCCAGG TGGAGTCTCT TGAAGGTAGG ATTGGCCGGC TCTGCAAGGA AAGTCCCGAG 
GTTGCCCACA GCCTCAGACA TAAGCAGCAA GAAATGATGG ACAGCTGGTG GAAGGTCTGG 
AGCAAAGCCC AGAACAGGAG GGAGTTGCTG GATGTGGGCC ATGAAGTTCA GAAACTCCAG 
ACCCTGCTGC AGGATCTGCA GGACTGGGCC CGGGGACTAC AGGCTGAGAT GGCCACACAG 
GGCACCCCCT GCAGCCCTGT GAGAATCCAG TACATGTTGG AAGAGTACCG GGCATACAAG 
GTTGAGCTGG ACATCCGAAC AGAGAGCCTT AACCTGGTCC AAAGCATGGG GCAGCGGCTG 
CTTGCATCTG GCTACCCACA GGCCTCTGAG ATTCACCAGA CCCTGGCCGC TGTAGAGCAG 
GGGTTGAGTA GCTTGCGGGA ATCCTGGCAG GGCCGGCAAC AGCAGCTACA GCAGGCCTTG 
GAGCAGCAGC TGTTTCTGGG CTCTGTGGAA AAGGCGGAAC GCTGGCTTGA CAGTGAAGAG 
GCCTCCCTGG CTAGTGAGGG AGTGGCGGAC CCCTTGGTCA CTGTGGAGAC CCTTCTGTCA 
AAGCTCAAGA GGCATGAGCA GGGCCTGAAG GCCCAGGCCG AAAAGATCCG TGCATTGGAG 
GCCACAGCCC ACAGCCTGCA CCAGGGTGGA CACTCTGAGG CGCGTAGCAT CCTGGACAGA 
TGCCAGGCCC TGCTCCTGAG GACAGAGGCA CTCACAGAGC AAGCGAGGGC CCGGGGACAC 
CAGCTGGAGG AGCTGCGGAA GCTTCGGACA TTTTTGCAGG ATTCCAATGA GGTAACTGCA 
TGGCTGAGGG AGAAGAGCCT GGCGGCTCTG GATGAGGGCC AGCAGGACCC AGCCACGATG 
CAAACACAGT TGCAGAAGCA GCAGAATTTC CAGGCTGAGC TGGACGCCAG TGTTCACCAA 
CAGCAGGAGG TCCAGATGGA GGGGCAGAAG CTGCTACAAG GGGGCCACCC GGCCTCAGAG 
ACCATCCGGG GGCAACTGGA AGAATTAGGA GGCCTCTGGG ATGAGCTGCA GACCAACTGC 
CAGAGGAAGA TGGCCAGACT ACAGGGGGCC CTCAAGGTCC TGCATCTGCA GAGAATGCTG 
AAGGAACTGG AGAAGTGGCT GGAGCACATG GAAGCAGAGC TGAGAGTCCC TGTCAGAAGC 
CAGGCGCTGC CTAGAGTGGG CGAACTCCTG GGAGCCCAGG AGGAGCTGGA AGCAGCCATG 
GACAGGCAGG CCAAGGAAGT GCAAGAGTTG CAGGGCCAGT CCCAAGCCTG CCTCCAGGAA 
GGCCACTGCC TCGCCAAAGA TGTGGAAGAG CAAGCCCGGC AGCTGCTTCA GAGGTTTCAG 
AGCCTTCGGG AGCCCCTACA AGAGCGCAGG GCATCCCTGG AAGCCCAAAG GCTCCTCTTA 
CAGTTCTTCA GGGATGCTGA TGAGGAAATG GCCTGGGTGC AGGAGAAGCT GCCCTCAGCC 
ACAGCCCAGG ACTACGGCCA GAGCCTTAAC ACTGTGCGCC ACCTGCAGGA GAAACACCAG 
AATTTGGAGA ATGAGATCCA TAGCCACAAG GCTCTGAGCC AGGTGGTGAC AGGTACAGGG 
CACAAGCTCA TACAGGCAGG GCACTTTGCC ACTGAAGAGG TGGCCGCCCG GGTGCAGCAG 
CTGGAGGTGG CTCTAAACCG CTTAGAGACA GAGGCCGCAC AGAGGAGGAG GAGACTGCAG 
CAGGCCCTGG AGGCCCAGCA GACTTTGGTG GAGCTCCTGG AAGCAGGATC CTGGCTGGCT 
GAACGGGACC ATATTCTGGA CAGTGAGGAC CTGGGCCAGG ACGCTGAGGC CACACAGGCT 
CTTCTGCGGT GCCTAGAGGC CACCACAAGA GACTTGGAAG GGTTCAGCAG TCGCATTGAG 
CAGTTACAGC AAACAGTTGC CCTTCTGGAG AGTGGACAGA CCCCAGGCAG TCCTAGGGTG 
CTGGCCCAGC TGCAGGCTGT GAGAGAGGCC CATGCACGGC TGCTGCAGAG AGCAGAAAGC 
AGGGGGGAGG CGCTGCGTGA ACAGCTGCAC CTGTACCAGC TGGAGCAAGA GGCCTTGCTC 
CTGGATGCCT GGCTGACCAC CAAGCTGGCC GTCGCTGAGT CTCAGGACTA TGGACAGGAT 
CTAGCAGGCA TCAAGGTGCT GGAAGACATG TTTGGTGCTT TCAACAGAGA AGTCCAGAGC 
CTGGGCCAGG CCAAGATGCA GACCCTGAGG GAGCGGATGG CTTCCCTAGA AAGAGGAGCA 
CCCAGGTTCT ACCCCCAGAT TCAAGCTCAG AAGTGTCGCG TCCAGGCCGC TTGGGAGGGG 
CTGAACAAGG CAATCAAAGT CCGTACAGAG AACCTGGCAG CAGCCTGCGA TCTCCGGAGC 
TTTGAGCAGG CTGCCTCGGA GCTGCAGAGA TGGATACAGG AGAAGACCAC CCTGCTGGAG 
GAAGCATTCC AAGTCCACAG CCTGTCACCT TCACAGCCCC TTCTGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCATAGGC GCCTGCAGAG AGAACTGAGG GCTATTGAAA AAGAGGTGTC AAGAGTGCAG 
ATGGAGGCCC ATCGGCTCGG CCAGCACTAC CCTGTGGCTC AGGGGAGTCT TAGTGAGTGG 
CTCACCAAGG TGCAGGGGGC CTGGACCAAC CTGGAGGCCA AAGTCCAGGA ATGGAGCCAG 
AAGCTGTTGC AGGCTACCCA GGGCCACACC TTTCTTGGGA GCTGCCGGGA ATTGCTAGCG 
TGGGCTCAGG AGATGCAGGA GCTGCTTTCT AAGGAGAAGC AGGCTGGGGA TGTGGTGGGG 
GCCAAGCAGT TCCTTGAGCA GCATGAGGCG CTGGAGCAAG AGATTCAGGA ACGCTGCCTT 
CAAGCCCAGA CCATACGGCA CGAAGGGCAG CAGCTGCTGG ACAACGGCCA CTTCCTGTCC 
CCAGAGGTGG CAGAGTGTAT GCAGGAGCTG GAAAGACATC TGCAGGAGCT TCAGGTAGCC 
TGGGCCCTGC GTGGACAACG CTGGGAACAG ACCCGGAGCC TGCAACAGCT GCGGCAGAGG 
CTGGAGCTGG CTGAGGCTTG GCTGGCCTCC TGGGAGCGCC TGCTGCTGGA CCCCAGCTGT 
GGGCACTCGG TGTTGGAAGT GGAACGCCTG CTCTACAGAC ACGAGGGCTT AGAAAAGCTG 
CTGGTGGCCC ACGAGGAGAC ATTCATTCAG CTTCAGACGA TGACAGAGGA GGCAAAGGGT 
GGTCATGTCA CGGAGGCATC TGTGGAACAG CAGCCCCCCA CAGAAGGGAG GCAGCCCAGA 
ACCAGCTTCC CAACCAGCAG CCATGGGATC TTGGCAAGGG GAGCCTCGAG CCCATTCTCA 
GATATGAGGA AGGCAGAAGT GCCAAGTGGC ACTGGGGAAC TGGCTTACGT GTCCCCCCTT 
GTACCTGAGG AGACTGAATG TGAGAGGCTG GAGGACAGAA GGCAGACTTT CTTCCCAAGG 
TCTAAACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357867.1
CDS	2866..13737
Translation	MDSEYEIGHVRKLQAQHTHMQEKTFTNWINNIFRLGRVGIRIQNLYTEFADGAHLLRLLELISGEALPAPSPGRLRVHFLENNSHALAFLRAKVPIPFIGPENIVDGDQSLILGLLWVIILRFQISHISLDREEFGASAALLSAKEALLVWCQRKTAGYTNVDITDFSRSWSDGLGFNALLHAHRPDLLDYGSLSPDRPLYNLSFAFRVAEQQLGIAQLLDPEDVAALHPDECSIMTYLSQYYHYFSRLQRGHTAQRRLAKILLQLQETEVLQAQYKQLVADLLCWIEEKKMQLEARDFPDSLPAMRQLLAAFASFRAREKPPRWQQRGATEALLFQLQTTLRAQNRRPFLPREGLGPAELAQRWAELERAEACRSQAMQQRLLQLERLDTLARRFQCKASHRESFLNDVEQMLDQARASLTDPATVEAATQRLSVLEAAILPQEGRFQALGEMADILRQEEYHSWADMARRQKEITGRWRRLLRCLQEERKRMEDSKAVLSLLQGVEAVTHQLGELQVLASSTVYGQQLAEIVSLLQSHDLLEAQVSAHRTHVTHLVHQTTQLDSQGTSVEVLQAKALALAELHHSLVSLVRARRTLLEQTLQRAQFLRSCEEEEAWLQEHRQLMETAVLDRDLTQIATALQKHKALETELHRHQAVCVDLMQRGRNFSVREPLTQPDPLERAEAVQGTWQLLWAGAARRRARLQTALLIGQYFSDSAEAASWLFQRQKQLESASCGKDQADAEALLLQHLRLEQDVRAFAAELRELEEQARAAAALVSHMVHTGTWKGSRLVSPTPNEEPPEDWKRLGRDTIGGPQGETQVLAAPGSHYSHQQIAFFSREEAPNTWKLEELGDSQSRMAIIHSIEKKPQVLSALGFLGEGPRTVLQAENDVHFNSSTILQAQADVSQTYEKLRALAKLHRTRLEESIALFSFYSSCRELQSWLEKQTALFQTLQPQGHNLEVIQLKYENVLMTLATGKGHWAEAINTAEQLKQRCPGHISKIQQQQEDLKQRWQQLEALKEEKLLQLTRGMEVCSLLQESEPIQAQLLNVISRLETLGTRSSGDSHHTLQQTQQKVLVLEKKISYLQRATIEVMESGSPESRLLWEQVLMLQSLLKQAQGQVARQIQVQTVARVQRGILQESQKLLLWAEDIQAQLCSKEERQVEASALRPLRRHGTLQEETCLWEERLQQLEAQGQPVAVSDSPQSQEVASALRLLGQHSQQLKALWEQRQQKFWEGLELQGFGQEVDDFMATCASHEALLQLDNLGEDIREAQSLLQQHQGLGWLRSTLGSRAEALRARGEKLLLSHPAAVSKIRELLHSAQAQWTRVQERSEQRRVQLLASLQLQEWKQAEEGLMLWMEEKWPRVADERSQAGSNILQKLKWRKITKSELLASRRYMEELQQAGKELLHSNPYAQEDIQDRLQSLNHKWEELNHKMEDRGDRLPQTRQQDQLLELIQDVQETMEHLEGALQSTETGQDLCSSRRLQRQHCKLEDKSQALASKMDALISQTHNAFTSWTILEESQKCHQRFKSLQSKLATQHQQLQASVELYEFNLLSNLELTWAAEHMPNAALNCPAQCWHDAHSLQRKHKMLQAEVKGHVRHMYRVLSSGQRLAASGHPRAQHIVEQCQKLESHWAGLEQACEERAHCLQQAVTVQQYFLNVSEMETWVEEKRPLASSQDYGSNEEATSGLIRKHQMLQQEVALYWSSMEDLEQRFQTLAEFEAPERLGVVREKLQALRKLADERGQELEGTLRLHEFMREAEDLQSWLSSRKQVARGGDTFGEDHEHVLQLCTEFTKFQYQVETGAQRVETCRLLAESLQERGHSAAPKAHQRQQDIQASWSELCQLTQARSRLLNDAEITLRVHGDLLEVLTQIQEKATSLPNDVAQDLCGVENQLQRHERLERELAGMEQQVQELMKAGGRVQELCPGTQALAAVQQKQQAVTQAWEALQLRMEQRKAQLERGYLLVRFHTAVRDYTSWAASVHQELQMEEASWEPHSLLLRLRAHQWLWAELKAKEELQQRATKMGQQALLAAGTPAKVQDGLRTLQEERDQVFQAWALKQEKLQAMLQEQHLLRQCGHLVEVLTAQEAFLKASGLGSSVEEVEQLIRKHVIFQKVLALQDKKESALNEQLKTISSPKGQNLFCHMLEHRAQVKELAESRGQALHTSLMIGNFVRAATQAEDWIQQRVQQLRAWSPLGNLKDYLKHLRKHQAFRAEVQAQEQILTSVAKQGEELLSQSHPQAGEVSQRLEALRDLWEKLRQAVTLQGQALENRYNFQEFLQRVDLAETWIQEKERMVNSCDIGLNLEHCLQLCRQVRRLQVTVDDAHIKGIKNLSLQLKNQGPEESETICQRQNQLNNRWKTFHGNLLLYQQRLEAALEIHRLSRELDDVIERLREKESLIWAPEGTEDLENVQRLSWRQKVLQQEMGLIQTQVESLEGRIGRLCKESPEVAHSLRHKQQEMMDSWWKVWSKAQNRRELLDVGHEVQKLQTLLQDLQDWARGLQAEMATQGTPCSPVRIQYMLEEYRAYKVELDIRTESLNLVQSMGQRLLASGYPQASEIHQTLAAVEQGLSSLRESWQGRQQQLQQALEQQLFLGSVEKAERWLDSEEASLASEGVADPLVTVETLLSKLKRHEQGLKAQAEKIRALEATAHSLHQGGHSEARSILDRCQALLLRTEALTEQARARGHQLEELRKLRTFLQDSNEVTAWLREKSLAALDEGQQDPATMQTQLQKQQNFQAELDASVHQQQEVQMEGQKLLQGGHPASETIRGQLEELGGLWDELQTNCQRKMARLQGALKVLHLQRMLKELEKWLEHMEAELRVPVRSQALPRVGELLGAQEELEAAMDRQAKEVQELQGQSQACLQEGHCLAKDVEEQARQLLQRFQSLREPLQERRASLEAQRLLLQFFRDADEEMAWVQEKLPSATAQDYGQSLNTVRHLQEKHQNLENEIHSHKALSQVVTGTGHKLIQAGHFATEEVAARVQQLEVALNRLETEAAQRRRRLQQALEAQQTLVELLEAGSWLAERDHILDSEDLGQDAEATQALLRCLEATTRDLEGFSSRIEQLQQTVALLESGQTPGSPRVLAQLQAVREAHARLLQRAESRGEALREQLHLYQLEQEALLLDAWLTTKLAVAESQDYGQDLAGIKVLEDMFGAFNREVQSLGQAKMQTLRERMASLERGAPRFYPQIQAQKCRVQAAWEGLNKAIKVRTENLAAACDLRSFEQAASELQRWIQEKTTLLEEAFQVHSLSPSQPLLQQQQQQQQQQQQQQQQQQQQQQQQQQHRRLQRELRAIEKEVSRVQMEAHRLGQHYPVAQGSLSEWLTKVQGAWTNLEAKVQEWSQKLLQATQGHTFLGSCRELLAWAQEMQELLSKEKQAGDVVGAKQFLEQHEALEQEIQERCLQAQTIRHEGQQLLDNGHFLSPEVAECMQELERHLQELQVAWALRGQRWEQTRSLQQLRQRLELAEAWLASWERLLLDPSCGHSVLEVERLLYRHEGLEKLLVAHEETFIQLQTMTEEAKGGHVTEASVEQQPPTEGRQPRTSFPTSSHGILARGASSPFSDMRKAEVPSGTGELAYVSPLVPEETECERLEDRRQTFFPRSKP

Target ORF information:

RefSeq Version	NM_001370938.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370938.1

ORF Insert Sequence:

ATGGACTCTG AGTATGAGAT AGGCCATGTC CGCAAGCTCC AGGCTCAGCA TACACACATG 
CAGGAGAAGA CCTTCACCAA CTGGATCAAT AACATCTTCC GACTAGGACG GGTGGGCATC 
AGGATCCAGA ACCTGTACAC AGAGTTTGCT GACGGTGCCC ACCTACTGCG GCTATTGGAG 
CTTATCTCTG GGGAGGCCCT ACCAGCGCCC AGTCCGGGCC GCCTGCGCGT GCACTTCCTG 
GAGAACAACA GCCACGCCCT GGCCTTCCTC AGGGCCAAGG TGCCCATTCC CTTCATCGGG 
CCAGAGAACA TTGTGGATGG GGACCAGTCA CTCATCCTGG GACTCCTCTG GGTCATCATT 
CTGCGTTTCC AGATTTCCCA CATCTCCTTG GACAGGGAGG AGTTTGGGGC CAGTGCGGCT 
TTGCTGTCTG CCAAGGAAGC CCTGCTGGTC TGGTGTCAGC GGAAGACAGC CGGCTACACC 
AATGTGGACA TCACTGACTT CTCCCGAAGC TGGAGTGACG GGCTCGGCTT TAATGCCCTG 
CTCCACGCTC ACAGGCCGGA CCTGCTGGAT TATGGCTCGC TGAGTCCAGA CCGCCCTCTG 
TATAACCTCT CCTTTGCCTT CCGAGTGGCT GAGCAGCAGC TTGGCATTGC CCAGCTGCTC 
GACCCTGAGG ATGTGGCAGC CCTGCATCCA GATGAGTGCT CCATCATGAC CTACCTGTCT 
CAGTACTACC ACTACTTCTC CCGTCTGCAG CGGGGGCACA CGGCCCAGAG GAGACTGGCT 
AAGATCCTGC TGCAGCTGCA GGAGACGGAG GTATTGCAGG CACAATACAA GCAACTGGTG 
GCTGACCTGC TCTGCTGGAT TGAGGAGAAG AAGATGCAGC TAGAAGCTCG GGACTTCCCA 
GACTCACTGC CTGCCATGCG CCAGCTACTG GCAGCCTTTG CCTCCTTCCG TGCCCGGGAA 
AAGCCACCTC GGTGGCAGCA GCGAGGAGCC ACAGAGGCCC TGCTCTTCCA GCTGCAGACA 
ACACTCCGAG CCCAAAACCG AAGGCCATTC CTACCTCGAG AGGGCCTGGG CCCTGCAGAG 
CTGGCCCAGC GCTGGGCAGA ACTGGAGAGG GCAGAGGCCT GCAGGAGCCA GGCCATGCAG 
CAAAGGCTGC TACAGCTGGA GCGACTGGAC ACTCTGGCCC GCCGCTTTCA GTGCAAGGCA 
TCCCACCGGG AAAGCTTTTT AAATGACGTA GAGCAGATGT TAGACCAGGC CAGAGCCTCC 
CTCACCGACC CAGCCACAGT GGAGGCAGCC ACTCAGAGGC TGAGCGTTCT GGAGGCTGCC 
ATCCTGCCCC AGGAAGGGCG CTTCCAGGCC TTGGGTGAGA TGGCAGACAT CCTCCGGCAG 
GAAGAGTATC ACAGCTGGGC GGATATGGCC CGCAGGCAGA AGGAGATCAC GGGGCGCTGG 
CGGAGGCTCC TACGGTGTCT ACAGGAGGAG AGGAAGCGCA TGGAAGACTC AAAGGCTGTG 
CTGAGTCTGC TGCAGGGGGT GGAGGCTGTC ACTCACCAGC TTGGGGAGCT GCAGGTGTTG 
GCCAGCTCCA CAGTCTATGG GCAACAGCTG GCAGAAATAG TGTCGCTGCT GCAGAGCCAT 
GACCTGCTGG AGGCCCAGGT GTCAGCCCAC AGGACCCATG TGACTCATCT TGTCCACCAG 
ACCACCCAGC TAGACTCCCA GGGTACCAGT GTGGAGGTGC TGCAGGCTAA AGCCCTAGCA 
CTGGCCGAGC TCCACCACAG CCTGGTGTCT CTTGTCAGAG CCCGACGTAC CCTGCTGGAG 
CAGACCCTCC AGCGAGCACA GTTTCTGCGC AGCTGTGAGG AGGAGGAGGC CTGGTTGCAA 
GAGCACAGAC AGCTAATGGA GACCGCGGTC CTGGACCGGG ACCTCACTCA GATCGCTACT 
GCTCTGCAGA AACACAAGGC TCTGGAAACA GAACTCCATC GCCACCAGGC AGTGTGTGTA 
GATCTCATGC AGAGAGGACG CAACTTCAGT GTCAGAGAGC CCCTGACACA GCCAGATCCA 
CTGGAAAGGG CAGAAGCAGT GCAGGGAACC TGGCAGCTGC TCTGGGCTGG AGCCGCAAGG 
CGGCGCGCCC GGCTGCAAAC AGCACTGCTA ATTGGGCAGT ACTTTTCTGA CTCGGCCGAA 
GCAGCTTCCT GGCTTTTCCA GCGGCAGAAA CAACTAGAGA GCGCATCCTG CGGGAAGGAC 
CAGGCAGACG CCGAGGCCCT GTTGCTGCAA CACCTGCGCC TGGAGCAAGA CGTGCGCGCC 
TTCGCGGCGG AGCTGCGCGA GCTAGAGGAG CAGGCGCGGG CTGCCGCAGC CCTAGTGTCA 
CACATGGTGC ACACGGGGAC ATGGAAAGGA TCCAGACTTG TCTCTCCTAC ACCAAATGAG 
GAACCTCCAG AAGACTGGAA AAGGCTTGGC AGAGACACCA TTGGAGGACC CCAGGGGGAG 
ACTCAAGTTT TAGCAGCACC AGGCAGCCAT TATTCACATC AGCAGATAGC CTTCTTCTCC 
AGAGAAGAGG CTCCAAATAC CTGGAAGCTT GAAGAGTTGG GAGACAGTCA GAGCAGGATG 
GCCATCATCC ACAGCATCGA GAAGAAACCC CAGGTGCTAT CTGCCCTGGG CTTTCTAGGA 
GAAGGCCCGA GGACAGTTCT GCAGGCTGAG AATGACGTTC ACTTCAACTC CAGCACCATA 
CTCCAGGCTC AGGCTGACGT GAGCCAGACC TATGAGAAGC TTAGGGCCCT GGCAAAGCTT 
CACAGGACCC GACTGGAGGA GTCAATCGCC CTGTTTAGCT TTTATAGCTC CTGTAGGGAG 
CTTCAGTCCT GGCTGGAGAA GCAGACCGCA CTGTTCCAAA CATTGCAGCC CCAGGGACAC 
AACTTAGAGG TCATACAACT CAAATATGAG AATGTTCTCA TGACCTTGGC CACTGGAAAA 
GGCCACTGGG CAGAGGCCAT CAACACTGCT GAACAACTGA AGCAGAGATG TCCAGGACAC 
ATCTCGAAAA TCCAACAACA ACAGGAGGAC CTGAAGCAAA GGTGGCAGCA GCTGGAAGCC 
CTGAAGGAGG AGAAACTCCT GCAGCTGACA CGTGGCATGG AGGTGTGCAG TCTTTTACAG 
GAGTCTGAAC CCATACAAGC CCAACTGCTA AATGTGATAA GCAGGCTGGA GACTCTGGGA 
ACAAGAAGCT CTGGGGACAG CCATCATACC CTACAGCAGA CCCAGCAGAA GGTCCTGGTA 
TTGGAGAAGA AGATCTCCTA CCTCCAAAGA GCAACCATAG AGGTGATGGA GTCAGGCTCC 
CCAGAGAGCA GGCTGCTTTG GGAGCAGGTC CTGATGCTGC AGTCACTGCT AAAGCAAGCG 
CAAGGGCAGG TGGCCCGGCA GATCCAGGTC CAGACTGTGG CTCGGGTTCA GCGAGGCATC 
CTCCAGGAGA GCCAGAAGCT GCTGCTGTGG GCAGAGGACA TCCAGGCTCA GCTGTGCAGC 
AAAGAGGAGC GGCAGGTTGA GGCTTCAGCC CTGCGGCCGC TGAGGAGGCA TGGAACCCTA 
CAGGAGGAGA CCTGCCTGTG GGAAGAGAGG CTGCAGCAGC TGGAGGCTCA GGGTCAGCCG 
GTGGCAGTCT CAGACTCTCC ACAGTCCCAG GAGGTGGCCA GCGCTCTAAG GCTCCTAGGC 
CAGCACAGCC AGCAGCTGAA GGCTCTGTGG GAGCAGAGAC AGCAGAAGTT TTGGGAGGGG 
CTGGAGCTGC AGGGGTTTGG CCAAGAAGTA GATGATTTCA TGGCCACCTG TGCCAGCCAT 
GAGGCCTTAC TGCAGCTGGA CAACCTTGGG GAGGACATAA GGGAGGCCCA GAGCCTGCTG 
CAGCAGCACC AGGGTTTGGG GTGGCTCCGC AGCACCCTGG GATCACGGGC AGAGGCCCTC 
CGTGCACGCG GTGAGAAGCT GCTTCTGAGC CATCCAGCTG CCGTGAGCAA GATCAGAGAG 
CTGCTGCACA GTGCCCAGGC CCAGTGGACC AGGGTCCAAG AGAGGAGTGA GCAGAGAAGG 
GTCCAGCTGC TGGCTTCCCT CCAATTGCAG GAATGGAAGC AGGCTGAAGA AGGCCTAATG 
CTGTGGATGG AGGAGAAGTG GCCCAGGGTG GCTGATGAGC GCTCCCAAGC AGGAAGCAAC 
ATTCTGCAGA AACTCAAGTG GCGCAAAATA ACTAAGAGTG AGCTATTAGC CTCTCGGAGG 
TACATGGAAG AGCTACAGCA GGCTGGGAAA GAACTGTTGC ATAGCAACCC ATATGCACAG 
GAGGACATAC AGGACAGACT TCAAAGCCTT AATCACAAAT GGGAAGAGTT GAACCACAAG 
ATGGAAGATC GAGGGGATAG GCTGCCTCAG ACCAGACAGC AGGACCAGCT CCTAGAACTA 
ATACAGGATG TCCAGGAGAC AATGGAGCAT CTGGAGGGAG CCCTGCAGAG CACAGAAACC 
GGGCAGGACC TGTGCTCCAG CCGGAGGCTG CAGAGACAGC ATTGCAAGCT AGAGGACAAG 
AGCCAGGCAC TGGCCAGCAA GATGGATGCC CTCATCTCTC AGACCCACAA TGCATTCACT 
TCCTGGACCA TCCTGGAGGA GAGCCAGAAG TGTCACCAGA GGTTCAAGTC CCTGCAGAGC 
AAGCTAGCCA CCCAACACCA GCAACTGCAG GCCTCAGTTG AGCTGTATGA ATTTAATCTC 
CTTAGCAACC TGGAGCTCAC ATGGGCAGCT GAGCACATGC CCAATGCCGC TCTCAACTGC 
CCTGCCCAGT GCTGGCATGA TGCCCACAGC CTTCAACGAA AGCACAAGAT GCTCCAGGCT 
GAGGTGAAAG GTCATGTAAG GCACATGTAC AGGGTGCTAA GTTCTGGTCA GCGTCTAGCA 
GCCTCTGGGC ACCCTCGAGC TCAGCATATC GTGGAACAGT GCCAGAAGCT AGAAAGCCAC 
TGGGCAGGAC TGGAGCAGGC GTGTGAAGAA CGTGCACACT GCCTGCAACA GGCTGTCACT 
GTCCAGCAGT ACTTTCTGAA CGTGTCAGAG ATGGAGACCT GGGTGGAGGA AAAGCGGCCT 
CTGGCAAGCA GTCAGGACTA CGGCAGTAAT GAGGAAGCTA CCAGTGGGCT CATTAGGAAA 
CACCAGATGC TCCAGCAGGA GGTAGCTCTT TATTGGAGCT CCATGGAAGA TCTCGAGCAG 
AGGTTCCAGA CCCTTGCTGA ATTTGAGGCC CCTGAGCGGT TGGGTGTGGT ACGGGAGAAG 
TTGCAGGCAC TACGGAAGCT GGCAGACGAA CGGGGGCAGG AGCTGGAGGG GACCCTGAGA 
CTACATGAGT TCATGAGAGA AGCTGAGGAC CTGCAGAGCT GGCTGTCCAG CAGGAAGCAG 
GTGGCCAGAG GAGGGGACAC CTTTGGAGAG GACCATGAGC ACGTGCTGCA ACTCTGCACT 
GAGTTTACAA AGTTTCAGTA CCAGGTTGAA ACTGGTGCCC AGCGAGTGGA AACCTGTCGA 
CTGCTGGCAG AGAGCCTACA AGAGCGTGGG CACAGTGCTG CCCCCAAGGC TCACCAGAGG 
CAGCAGGATA TACAGGCTTC CTGGTCTGAA CTGTGCCAGT TGACCCAGGC CCGAAGCCGT 
CTGCTCAATG ACGCCGAAAT CACTCTTAGA GTCCACGGAG ACCTTCTGGA AGTCCTTACC 
CAGATTCAGG AGAAAGCCAC AAGCCTCCCT AACGATGTGG CCCAGGACCT GTGTGGGGTA 
GAGAACCAGC TCCAGAGACA TGAGAGGCTG GAACGCGAGT TGGCAGGCAT GGAGCAGCAG 
GTGCAGGAAC TGATGAAGGC TGGAGGCAGA GTGCAGGAGC TGTGTCCAGG AACTCAGGCC 
CTTGCCGCTG TCCAGCAAAA GCAGCAAGCT GTGACCCAAG CTTGGGAGGC CCTCCAGCTG 
CGCATGGAGC AGCGCAAGGC TCAATTGGAA CGAGGATATC TCCTGGTCCG GTTCCACACA 
GCGGTGAGGG ACTATACCTC CTGGGCGGCC AGCGTGCATC AGGAGCTACA GATGGAGGAG 
GCCTCCTGGG AACCCCACAG CCTTTTGCTC AGGCTCAGAG CCCATCAATG GCTGTGGGCA 
GAACTGAAAG CCAAGGAGGA GCTGCAGCAA CGGGCCACTA AGATGGGCCA GCAAGCTCTG 
CTGGCAGCAG GAACACCCGC TAAGGTCCAG GATGGGCTTC GAACCCTACA GGAGGAACGC 
GACCAAGTGT TCCAGGCCTG GGCACTCAAG CAAGAGAAGC TGCAGGCCAT GCTTCAAGAA 
CAGCACCTCC TCAGGCAATG CGGCCACCTA GTGGAGGTCC TCACAGCCCA GGAGGCATTC 
CTAAAAGCCA GTGGCCTGGG GAGCTCAGTG GAAGAAGTGG AGCAGTTGAT CCGAAAGCAC 
GTGATCTTCC AGAAAGTGCT AGCTCTCCAG GACAAGAAGG AGTCGGCTCT GAATGAGCAG 
CTAAAGACGA TTTCAAGCCC TAAGGGGCAG AACCTGTTTT GCCACATGTT GGAGCACCGG 
GCTCAAGTGA AGGAACTGGC AGAGAGCCGG GGCCAGGCTC TGCACACCTC CCTGATGATA 
GGCAACTTTG TCAGGGCAGC AACCCAGGCT GAGGACTGGA TCCAGCAGCG GGTCCAGCAA 
CTGAGAGCCT GGAGCCCTCT TGGGAATCTA AAGGATTATC TGAAGCACCT GAGGAAACAC 
CAGGCCTTCA GGGCTGAGGT CCAGGCTCAG GAGCAGATCT TGACCTCTGT TGCCAAGCAA 
GGCGAAGAGC TCCTCAGTCA GAGCCACCCT CAAGCTGGAG AGGTCTCCCA GAGGCTGGAG 
GCCCTGCGGG ACCTCTGGGA GAAGCTGAGG CAGGCAGTGA CCCTGCAGGG CCAGGCCCTA 
GAGAACAGAT ACAACTTCCA GGAGTTCCTG CAGAGAGTGG ACCTAGCAGA GACCTGGATC 
CAGGAGAAGG AGAGGATGGT GAACAGCTGT GACATTGGCC TGAACCTTGA GCACTGCCTG 
CAGCTCTGCA GACAAGTCCG CAGGTTACAG GTCACAGTGG ATGATGCCCA CATCAAGGGA 
ATCAAAAACT TGTCACTGCA GCTCAAGAAC CAGGGCCCTG AGGAATCCGA GACCATCTGC 
CAGCGGCAAA ACCAGCTCAA CAATAGGTGG AAGACTTTCC ATGGCAACCT GCTCCTCTAT 
CAGCAGCGGC TTGAAGCAGC CCTGGAGATA CATAGATTGT CCAGGGAACT GGATGACGTT 
ATTGAGCGGC TCAGGGAGAA GGAATCCCTG ATCTGGGCCC CAGAGGGCAC TGAAGATTTG 
GAGAATGTGC AGAGGCTATC ATGGAGACAG AAAGTGCTGC AGCAGGAGAT GGGCCTCATC 
CAGACCCAGG TGGAGTCTCT TGAAGGTAGG ATTGGCCGGC TCTGCAAGGA AAGTCCCGAG 
GTTGCCCACA GCCTCAGACA TAAGCAGCAA GAAATGATGG ACAGCTGGTG GAAGGTCTGG 
AGCAAAGCCC AGAACAGGAG GGAGTTGCTG GATGTGGGCC ATGAAGTTCA GAAACTCCAG 
ACCCTGCTGC AGGATCTGCA GGACTGGGCC CGGGGACTAC AGGCTGAGAT GGCCACACAG 
GGCACCCCCT GCAGCCCTGT GAGAATCCAG TACATGTTGG AAGAGTACCG GGCATACAAG 
GTTGAGCTGG ACATCCGAAC AGAGAGCCTT AACCTGGTCC AAAGCATGGG GCAGCGGCTG 
CTTGCATCTG GCTACCCACA GGCCTCTGAG ATTCACCAGA CCCTGGCCGC TGTAGAGCAG 
GGGTTGAGTA GCTTGCGGGA ATCCTGGCAG GGCCGGCAAC AGCAGCTACA GCAGGCCTTG 
GAGCAGCAGC TGTTTCTGGG CTCTGTGGAA AAGGCGGAAC GCTGGCTTGA CAGTGAAGAG 
GCCTCCCTGG CTAGTGAGGG AGTGGCGGAC CCCTTGGTCA CTGTGGAGAC CCTTCTGTCA 
AAGCTCAAGA GGCATGAGCA GGGCCTGAAG GCCCAGGCCG AAAAGATCCG TGCATTGGAG 
GCCACAGCCC ACAGCCTGCA CCAGGGTGGA CACTCTGAGG CGCGTAGCAT CCTGGACAGA 
TGCCAGGCCC TGCTCCTGAG GACAGAGGCA CTCACAGAGC AAGCGAGGGC CCGGGGACAC 
CAGCTGGAGG AGCTGCGGAA GCTTCGGACA TTTTTGCAGG ATTCCAATGA GGTAACTGCA 
TGGCTGAGGG AGAAGAGCCT GGCGGCTCTG GATGAGGGCC AGCAGGACCC AGCCACGATG 
CAAACACAGT TGCAGAAGCA GCAGAATTTC CAGGCTGAGC TGGACGCCAG TGTTCACCAA 
CAGCAGGAGG TCCAGATGGA GGGGCAGAAG CTGCTACAAG GGGGCCACCC GGCCTCAGAG 
ACCATCCGGG GGCAACTGGA AGAATTAGGA GGCCTCTGGG ATGAGCTGCA GACCAACTGC 
CAGAGGAAGA TGGCCAGACT ACAGGGGGCC CTCAAGGTCC TGCATCTGCA GAGAATGCTG 
AAGGAACTGG AGAAGTGGCT GGAGCACATG GAAGCAGAGC TGAGAGTCCC TGTCAGAAGC 
CAGGCGCTGC CTAGAGTGGG CGAACTCCTG GGAGCCCAGG AGGAGCTGGA AGCAGCCATG 
GACAGGCAGG CCAAGGAAGT GCAAGAGTTG CAGGGCCAGT CCCAAGCCTG CCTCCAGGAA 
GGCCACTGCC TCGCCAAAGA TGTGGAAGAG CAAGCCCGGC AGCTGCTTCA GAGGTTTCAG 
AGCCTTCGGG AGCCCCTACA AGAGCGCAGG GCATCCCTGG AAGCCCAAAG GCTCCTCTTA 
CAGTTCTTCA GGGATGCTGA TGAGGAAATG GCCTGGGTGC AGGAGAAGCT GCCCTCAGCC 
ACAGCCCAGG ACTACGGCCA GAGCCTTAAC ACTGTGCGCC ACCTGCAGGA GAAACACCAG 
AATTTGGAGA ATGAGATCCA TAGCCACAAG GCTCTGAGCC AGGTGGTGAC AGGTACAGGG 
CACAAGCTCA TACAGGCAGG GCACTTTGCC ACTGAAGAGG TGGCCGCCCG GGTGCAGCAG 
CTGGAGGTGG CTCTAAACCG CTTAGAGACA GAGGCCGCAC AGAGGAGGAG GAGACTGCAG 
CAGGCCCTGG AGGCCCAGCA GACTTTGGTG GAGCTCCTGG AAGCAGGATC CTGGCTGGCT 
GAACGGGACC ATATTCTGGA CAGTGAGGAC CTGGGCCAGG ACGCTGAGGC CACACAGGCT 
CTTCTGCGGT GCCTAGAGGC CACCACAAGA GACTTGGAAG GGTTCAGCAG TCGCATTGAG 
CAGTTACAGC AAACAGTTGC CCTTCTGGAG AGTGGACAGA CCCCAGGCAG TCCTAGGGTG 
CTGGCCCAGC TGCAGGCTGT GAGAGAGGCC CATGCACGGC TGCTGCAGAG AGCAGAAAGC 
AGGGGGGAGG CGCTGCGTGA ACAGCTGCAC CTGTACCAGC TGGAGCAAGA GGCCTTGCTC 
CTGGATGCCT GGCTGACCAC CAAGCTGGCC GTCGCTGAGT CTCAGGACTA TGGACAGGAT 
CTAGCAGGCA TCAAGGTGCT GGAAGACATG TTTGGTGCTT TCAACAGAGA AGTCCAGAGC 
CTGGGCCAGG CCAAGATGCA GACCCTGAGG GAGCGGATGG CTTCCCTAGA AAGAGGAGCA 
CCCAGGTTCT ACCCCCAGAT TCAAGCTCAG AAGTGTCGCG TCCAGGCCGC TTGGGAGGGG 
CTGAACAAGG CAATCAAAGT CCGTACAGAG AACCTGGCAG CAGCCTGCGA TCTCCGGAGC 
TTTGAGCAGG CTGCCTCGGA GCTGCAGAGA TGGATACAGG AGAAGACCAC CCTGCTGGAG 
GAAGCATTCC AAGTCCACAG CCTGTCACCT TCACAGCCCC TTCTGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCATAGGC GCCTGCAGAG AGAACTGAGG GCTATTGAAA AAGAGGTGTC AAGAGTGCAG 
ATGGAGGCCC ATCGGCTCGG CCAGCACTAC CCTGTGGCTC AGGGGAGTCT TAGTGAGTGG 
CTCACCAAGG TGCAGGGGGC CTGGACCAAC CTGGAGGCCA AAGTCCAGGA ATGGAGCCAG 
AAGCTGTTGC AGGCTACCCA GGGCCACACC TTTCTTGGGA GCTGCCGGGA ATTGCTAGCG 
TGGGCTCAGG AGATGCAGGA GCTGCTTTCT AAGGAGAAGC AGGCTGGGGA TGTGGTGGGG 
GCCAAGCAGT TCCTTGAGCA GCATGAGGCG CTGGAGCAAG AGATTCAGGA ACGCTGCCTT 
CAAGCCCAGA CCATACGGCA CGAAGGGCAG CAGCTGCTGG ACAACGGCCA CTTCCTGTCC 
CCAGAGGTGG CAGAGTGTAT GCAGGAGCTG GAAAGACATC TGCAGGAGCT TCAGGTAGCC 
TGGGCCCTGC GTGGACAACG CTGGGAACAG ACCCGGAGCC TGCAACAGCT GCGGCAGAGG 
CTGGAGCTGG CTGAGGCTTG GCTGGCCTCC TGGGAGCGCC TGCTGCTGGA CCCCAGCTGT 
GGGCACTCGG TGTTGGAAGT GGAACGCCTG CTCTACAGAC ACGAGGGCTT AGAAAAGCTG 
CTGGTGGCCC ACGAGGAGAC ATTCATTCAG CTTCAGACGA TGACAGAGGA GGCAAAGGGT 
GGTCATGTCA CGGAGGCATC TGTGGAACAG CAGCCCCCCA CAGAAGGGAG GCAGCCCAGA 
ACCAGCTTCC CAACCAGCAG CCATGGGATC TTGGCAAGGG GAGCCTCGAG CCCATTCTCA 
GATATGAGGA AGGCAGAAGT GCCAAGTGGC ACTGGGGAAC TGGCTTACGT GTCCCCCCTT 
GTACCTGAGG AGACTGAATG TGAGAGGCTG GAGGACAGAA GGCAGACTTT CTTCCCAAGG 
TCTAAACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn5 ( XM_006500537.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500600.1 Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), mRNA.

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CloneID	OMu31110
Clone ID Related Accession (Same CDS sequence)	XM_006500537.2
Accession Version	XM_006500537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006500537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGGACAG TGTATCAAGA TAGGGCCTCA GACTCCTGGC AGAAGGATCA TCAGTTTCCA 
CAGTACTTCC CAGCCCCCAT GCCTCCACCA CCTCCAGACA CAGGGAAATG CCCAGTCGCC 
ATTCACCCTC AAAGAGCTAA GGCTCATAAG CTGGTTGTCC CATCAACCCT CCACCTCCTT 
AGCCGCAGCG TCCCTGACGA GAAGACCCAG GCCCAAAGCT CAGCCCTTCT GAGTTCAAGT 
TTGGCCATGG ACTCTGAGTA TGAGATAGGC CATGTCCGCA AGCTCCAGGC TCAGCATACA 
CACATGCAGG AGAAGACCTT CACCAACTGG ATCAATAACA TCTTCCGACT AGGACGGGTG 
GGCATCAGGA TCCAGAACCT GTACACAGAG TTTGCTGACG GTGCCCACCT ACTGCGGCTA 
TTGGAGCTTA TCTCTGGGGA GGCCCTACCA GCGCCCAGTC CGGGCCGCCT GCGCGTGCAC 
TTCCTGGAGA ACAACAGCCA CGCCCTGGCC TTCCTCAGGG CCAAGGTGCC CATTCCCTTC 
ATCGGGCCAG AGAACATTGT GGATGGGGAC CAGTCACTCA TCCTGGGACT CCTCTGGGTC 
ATCATTCTGC GTTTCCAGAT TTCCCACATC TCCTTGGACA GGGAGGAGTT TGGGGCCAGT 
GCGGCTTTGC TGTCTGCCAA GGAAGCCCTG CTGGTCTGGT GTCAGCGGAA GACAGCCGGC 
TACACCAATG TGGACATCAC TGACTTCTCC CGAAGCTGGA GTGACGGGCT CGGCTTTAAT 
GCCCTGCTCC ACGCTCACAG GCCGGACCTG CTGGATTATG GCTCGCTGAG TCCAGACCGC 
CCTCTGTATA ACCTCTCCTT TGCCTTCCGA GTGGCTGAGC AGCAGCTTGG CATTGCCCAG 
CTGCTCGACC CTGAGGATGT GGCAGCCCTG CATCCAGATG AGTGCTCCAT CATGACCTAC 
CTGTCTCAGT ACTACCACTA CTTCTCCCGT CTGCAGCGGG GGCACACGGC CCAGAGGAGA 
CTGGCTAAGA TCCTGCTGCA GCTGCAGGAG ACGGAGGTAT TGCAGGCACA ATACAAGCAA 
CTGGTGGCTG ACCTGCTCTG CTGGATTGAG GAGAAGAAGA TGCAGCTAGA AGCTCGGGAC 
TTCCCAGACT CACTGCCTGC CATGCGCCAG CTACTGGCAG CCTTTGCCTC CTTCCGTGCC 
CGGGAAAAGC CACCTCGGTG GCAGCAGCGA GGAGCCACAG AGGCCCTGCT CTTCCAGCTG 
CAGACAACAC TCCGAGCCCA AAACCGAAGG CCATTCCTAC CTCGAGAGGG CCTGGGCCCT 
GCAGAGCTGG CCCAGCGCTG GGCAGAACTG GAGAGGGCAG AGGCCTGCAG GAGCCAGGCC 
ATGCAGCAAA GGCTGCTACA GCTGGAGCGA CTGGACACTC TGGCCCGCCG CTTTCAGTGC 
AAGGCATCCC ACCGGGAAAG CTTTTTAAAT GACGTAGAGC AGATGTTAGA CCAGGCCAGA 
GCCTCCCTCA CCGACCCAGC CACAGTGGAG GCAGCCACTC AGAGGCTGAG CGTTCTGGAG 
GCTGCCATCC TGCCCCAGGA AGGGCGCTTC CAGGCCTTGG GTGAGATGGC AGACATCCTC 
CGGCAGGAAG AGTATCACAG CTGGGCGGAT ATGGCCCGCA GGCAGAAGGA GATCACGGGG 
CGCTGGCGGA GGCTCCTACG GTGTCTACAG GAGGAGAGGA AGCGCATGGA AGACTCAAAG 
GCTGTGCTGA GTCTGCTGCA GGGGGTGGAG GCTGTCACTC ACCAGCTTGG GGAGCTGCAG 
GTGTTGGCCA GCTCCACAGT CTATGGGCAA CAGCTGGCAG AAATAGTGTC GCTGCTGCAG 
AGCCATGACC TGCTGGAGGC CCAGGTGTCA GCCCACAGGA CCCATGTGAC TCATCTTGTC 
CACCAGACCA CCCAGCTAGA CTCCCAGGGT ACCAGTGTGG AGGTGCTGCA GGCTAAAGCC 
CTAGCACTGG CCGAGCTCCA CCACAGCCTG GTGTCTCTTG TCAGAGCCCG ACGTACCCTG 
CTGGAGCAGA CCCTCCAGCG AGCACAGTTT CTGCGCAGCT GTGAGGAGGA GGAGGCCTGG 
TTGCAAGAGC ACAGACAGCT AATGGAGACC GCGGTCCTGG ACCGGGACCT CACTCAGATC 
GCTACTGCTC TGCAGAAACA CAAGGCTCTG GAAACAGAAC TCCATCGCCA CCAGGCAGTG 
TGTGTAGATC TCATGCAGAG AGGACGCAAC TTCAGTGTCA GAGAGCCCCT GACACAGCCA 
GATCCACTGG AAAGGGCAGA AGCAGTGCAG GGAACCTGGC AGCTGCTCTG GGCTGGAGCC 
GCAAGGCGGC GCGCCCGGCT GCAAACAGCA CTGCTAATTG GGCAGTACTT TTCTGACTCG 
GCCGAAGCAG CTTCCTGGCT TTTCCAGCGG CAGAAACAAC TAGAGAGCGC ATCCTGCGGG 
AAGGACCAGG CAGACGCCGA GGCCCTGTTG CTGCAACACC TGCGCCTGGA GCAAGACGTG 
CGCGCCTTCG CGGCGGAGCT GCGCGAGCTA GAGGAGCAGG CGCGGGCTGC CGCAGCCCTA 
GTGTCACACA TGGTGCACAC GGGGACATGG AAAGGATCCA GACTTGTCTC TCCTACACCA 
AATGAGGAAC CTCCAGAAGA CTGGAAAAGG CTTGGCAGAG ACACCATTGG AGGACCCCAG 
GGGGAGACTC AAGTTTTAGC AGCACCAGGC AGCCATTATT CACATCAGCA GATAGCCTTC 
TTCTCCAGAG AAGAGGCTCC AAATACCTGG AAGCTTGAAG AGTTGGGAGA CAGTCAGAGC 
AGGATGGCCA TCATCCACAG CATCGAGAAG AAACCCCAGG TGCTATCTGC CCTGGGCTTT 
CTAGGAGAAG GCCCGAGGAC AGTTCTGCAG GCTGAGAATG ACGTTCACTT CAACTCCAGC 
ACCATACTCC AGGCTCAGGC TGACGTGAGC CAGACCTATG AGAAGCTTAG GGCCCTGGCA 
AAGCTTCACA GGACCCGACT GGAGGAGTCA ATCGCCCTGT TTAGCTTTTA TAGCTCCTGT 
AGGGAGCTTC AGTCCTGGCT GGAGAAGCAG ACCGCACTGT TCCAAACATT GCAGCCCCAG 
GGACACAACT TAGAGGTCAT ACAACTCAAA TATGAGAATG TTCTCATGAC CTTGGCCACT 
GGAAAAGGCC ACTGGGCAGA GGCCATCAAC ACTGCTGAAC AACTGAAGCA GAGATGTCCA 
GGACACATCT CGAAAATCCA ACAACAACAG GAGGACCTGA AGCAAAGGTG GCAGCAGCTG 
GAAGCCCTGA AGGAGGAGAA ACTCCTGCAG CTGACACGTG GCATGGAGGT GTGCAGTCTT 
TTACAGGAGT CTGAACCCAT ACAAGCCCAA CTGCTAAATG TGATAAGCAG GCTGGAGACT 
CTGGGAACAA GAAGCTCTGG GGACAGCCAT CATACCCTAC AGCAGACCCA GCAGAAGGTC 
CTGGTATTGG AGAAGAAGAT CTCCTACCTC CAAAGAGCAA CCATAGAGGT GATGGAGTCA 
GGCTCCCCAG AGAGCAGGCT GCTTTGGGAG CAGGTCCTGA TGCTGCAGTC ACTGCTAAAG 
CAAGCGCAAG GGCAGGTGGC CCGGCAGATC CAGGTCCAGA CTGTGGCTCG GGTTCAGCGA 
GGCATCCTCC AGGAGAGCCA GAAGCTGCTG CTGTGGGCAG AGGACATCCA GGCTCAGCTG 
TGCAGCAAAG AGGAGCGGCA GGTTGAGGCT TCAGCCCTGC GGCCGCTGAG GAGGCATGGA 
ACCCTACAGG AGGAGACCTG CCTGTGGGAA GAGAGGCTGC AGCAGCTGGA GGCTCAGGGT 
CAGCCGGTGG CAGTCTCAGA CTCTCCACAG TCCCAGGAGG TGGCCAGCGC TCTAAGGCTC 
CTAGGCCAGC ACAGCCAGCA GCTGAAGGCT CTGTGGGAGC AGAGACAGCA GAAGTTTTGG 
GAGGGGCTGG AGCTGCAGGG GTTTGGCCAA GAAGTAGATG ATTTCATGGC CACCTGTGCC 
AGCCATGAGG CCTTACTGCA GCTGGACAAC CTTGGGGAGG ACATAAGGGA GGCCCAGAGC 
CTGCTGCAGC AGCACCAGGG TTTGGGGTGG CTCCGCAGCA CCCTGGGATC ACGGGCAGAG 
GCCCTCCGTG CACGCGGTGA GAAGCTGCTT CTGAGCCATC CAGCTGCCGT GAGCAAGATC 
AGAGAGCTGC TGCACAGTGC CCAGGCCCAG TGGACCAGGG TCCAAGAGAG GAGTGAGCAG 
AGAAGGGTCC AGCTGCTGGC TTCCCTCCAA TTGCAGGAAT GGAAGCAGGC TGAAGAAGGC 
CTAATGCTGT GGATGGAGGA GAAGTGGCCC AGGGTGGCTG ATGAGCGCTC CCAAGCAGGA 
AGCAACATTC TGCAGAAACT CAAGTGGCGC AAAATAACTA AGAGTGAGCT ATTAGCCTCT 
CGGAGGTACA TGGAAGAGCT ACAGCAGGCT GGGAAAGAAC TGTTGCATAG CAACCCATAT 
GCACAGGAGG ACATACAGGA CAGACTTCAA AGCCTTAATC ACAAATGGGA AGAGTTGAAC 
CACAAGATGG AAGATCGAGG GGATAGGCTG CCTCAGACCA GACAGCAGGA CCAGCTCCTA 
GAACTAATAC AGGATGTCCA GGAGACAATG GAGCATCTGG AGGGAGCCCT GCAGAGCACA 
GAAACCGGGC AGGACCTGTG CTCCAGCCGG AGGCTGCAGA GACAGCATTG CAAGCTAGAG 
GACAAGAGCC AGGCACTGGC CAGCAAGATG GATGCCCTCA TCTCTCAGAC CCACAATGCA 
TTCACTTCCT GGACCATCCT GGAGGAGAGC CAGAAGTGTC ACCAGAGGTT CAAGTCCCTG 
CAGAGCAAGC TAGCCACCCA ACACCAGCAA CTGCAGGCCT CAGTTGAGCT GTATGAATTT 
AATCTCCTTA GCAACCTGGA GCTCACATGG GCAGCTGAGC ACATGCCCAA TGCCGCTCTC 
AACTGCCCTG CCCAGTGCTG GCATGATGCC CACAGCCTTC AACGAAAGCA CAAGATGCTC 
CAGGCTGAGG TGAAAGGTCA TGTAAGGCAC ATGTACAGGG TGCTAAGTTC TGGTCAGCGT 
CTAGCAGCCT CTGGGCACCC TCGAGCTCAG CATATCGTGG AACAGTGCCA GAAGCTAGAA 
AGCCACTGGG CAGGACTGGA GCAGGCGTGT GAAGAACGTG CACACTGCCT GCAACAGGCT 
GTCACTGTCC AGCAGTACTT TCTGAACGTG TCAGAGATGG AGACCTGGGT GGAGGAAAAG 
CGGCCTCTGG CAAGCAGTCA GGACTACGGC AGTAATGAGG AAGCTACCAG TGGGCTCATT 
AGGAAACACC AGATGCTCCA GCAGGAGGTA GCTCTTTATT GGAGCTCCAT GGAAGATCTC 
GAGCAGAGGT TCCAGACCCT TGCTGAATTT GAGGCCCCTG AGCGGTTGGG TGTGGTACGG 
GAGAAGTTGC AGGCACTACG GAAGCTGGCA GACGAACGGG GGCAGGAGCT GGAGGGGACC 
CTGAGACTAC ATGAGTTCAT GAGAGAAGCT GAGGACCTGC AGAGCTGGCT GTCCAGCAGG 
AAGCAGGTGG CCAGAGGAGG GGACACCTTT GGAGAGGACC ATGAGCACGT GCTGCAACTC 
TGCACTGAGT TTACAAAGTT TCAGTACCAG GTTGAAACTG GTGCCCAGCG AGTGGAAACC 
TGTCGACTGC TGGCAGAGAG CCTACAAGAG CGTGGGCACA GTGCTGCCCC CAAGGCTCAC 
CAGAGGCAGC AGGATATACA GGCTTCCTGG TCTGAACTGT GCCAGTTGAC CCAGGCCCGA 
AGCCGTCTGC TCAATGACGC CGAAATCACT CTTAGAGTCC ACGGAGACCT TCTGGAAGTC 
CTTACCCAGA TTCAGGAGAA AGCCACAAGC CTCCCTAACG ATGTGGCCCA GGACCTGTGT 
GGGGTAGAGA ACCAGCTCCA GAGACATGAG AGGCTGGAAC GCGAGTTGGC AGGCATGGAG 
CAGCAGGTGC AGGAACTGAT GAAGGCTGGA GGCAGAGTGC AGGAGCTGTG TCCAGGAACT 
CAGGCCCTTG CCGCTGTCCA GCAAAAGCAG CAAGCTGTGA CCCAAGCTTG GGAGGCCCTC 
CAGCTGCGCA TGGAGCAGCG CAAGGCTCAA TTGGAACGAG GATATCTCCT GGTCCGGTTC 
CACACAGCGG TGAGGGACTA TACCTCCTGG GCGGCCAGCG TGCATCAGGA GCTACAGATG 
GAGGAGGCCT CCTGGGAACC CCACAGCCTT TTGCTCAGGC TCAGAGCCCA TCAATGGCTG 
TGGGCAGAAC TGAAAGCCAA GGAGGAGCTG CAGCAACGGG CCACTAAGAT GGGCCAGCAA 
GCTCTGCTGG CAGCAGGAAC ACCCGCTAAG GTCCAGGATG GGCTTCGAAC CCTACAGGAG 
GAACGCGACC AAGTGTTCCA GGCCTGGGCA CTCAAGCAAG AGAAGCTGCA GGCCATGCTT 
CAAGAACAGC ACCTCCTCAG GCAATGCGGC CACCTAGTGG AGGTCCTCAC AGCCCAGGAG 
GCATTCCTAA AAGCCAGTGG CCTGGGGAGC TCAGTGGAAG AAGTGGAGCA GTTGATCCGA 
AAGCACGTGA TCTTCCAGAA AGTGCTAGCT CTCCAGGACA AGAAGGAGTC GGCTCTGAAT 
GAGCAGCTAA AGACGATTTC AAGCCCTAAG GGGCAGAACC TGTTTTGCCA CATGTTGGAG 
CACCGGGCTC AAGTGAAGGA ACTGGCAGAG AGCCGGGGCC AGGCTCTGCA CACCTCCCTG 
ATGATAGGCA ACTTTGTCAG GGCAGCAACC CAGGCTGAGG ACTGGATCCA GCAGCGGGTC 
CAGCAACTGA GAGCCTGGAG CCCTCTTGGG AATCTAAAGG ATTATCTGAA GCACCTGAGG 
AAACACCAGG CCTTCAGGGC TGAGGTCCAG GCTCAGGAGC AGATCTTGAC CTCTGTTGCC 
AAGCAAGGCG AAGAGCTCCT CAGTCAGAGC CACCCTCAAG CTGGAGAGGT CTCCCAGAGG 
CTGGAGGCCC TGCGGGACCT CTGGGAGAAG CTGAGGCAGG CAGTGACCCT GCAGGGCCAG 
GCCCTAGAGA ACAGATACAA CTTCCAGGAG TTCCTGCAGA GAGTGGACCT AGCAGAGACC 
TGGATCCAGG AGAAGGAGAG GATGGTGAAC AGCTGTGACA TTGGCCTGAA CCTTGAGCAC 
TGCCTGCAGC TCTGCAGACA AGTCCGCAGG TTACAGGTCA CAGTGGATGA TGCCCACATC 
AAGGGAATCA AAAACTTGTC ACTGCAGCTC AAGAACCAGG GCCCTGAGGA ATCCGAGACC 
ATCTGCCAGC GGCAAAACCA GCTCAACAAT AGGTGGAAGA CTTTCCATGG CAACCTGCTC 
CTCTATCAGC AGCGGCTTGA AGCAGCCCTG GAGATACATA GATTGTCCAG GGAACTGGAT 
GACGTTATTG AGCGGCTCAG GGAGAAGGAA TCCCTGATCT GGGCCCCAGA GGGCACTGAA 
GATTTGGAGA ATGTGCAGAG GCTATCATGG AGACAGAAAG TGCTGCAGCA GGAGATGGGC 
CTCATCCAGA CCCAGGTGGA GTCTCTTGAA GGTAGGATTG GCCGGCTCTG CAAGGAAAGT 
CCCGAGGTTG CCCACAGCCT CAGACATAAG CAGCAAGAAA TGATGGACAG CTGGTGGAAG 
GTCTGGAGCA AAGCCCAGAA CAGGAGGGAG TTGCTGGATG TGGGCCATGA AGTTCAGAAA 
CTCCAGACCC TGCTGCAGGA TCTGCAGGAC TGGGCCCGGG GACTACAGGC TGAGATGGCC 
ACACAGGGCA CCCCCTGCAG CCCTGTGAGA ATCCAGTACA TGTTGGAAGA GTACCGGGCA 
TACAAGGTTG AGCTGGACAT CCGAACAGAG AGCCTTAACC TGGTCCAAAG CATGGGGCAG 
CGGCTGCTTG CATCTGGCTA CCCACAGGCC TCTGAGATTC ACCAGACCCT GGCCGCTGTA 
GAGCAGGGGT TGAGTAGCTT GCGGGAATCC TGGCAGGGCC GGCAACAGCA GCTACAGCAG 
GCCTTGGAGC AGCAGCTGTT TCTGGGCTCT GTGGAAAAGG CGGAACGCTG GCTTGACAGT 
GAAGAGGCCT CCCTGGCTAG TGAGGGAGTG GCGGACCCCT TGGTCACTGT GGAGACCCTT 
CTGTCAAAGC TCAAGAGGCA TGAGCAGGGC CTGAAGGCCC AGGCCGAAAA GATCCGTGCA 
TTGGAGGCCA CAGCCCACAG CCTGCACCAG GGTGGACACT CTGAGGCGCG TAGCATCCTG 
GACAGATGCC AGGCCCTGCT CCTGAGGACA GAGGCACTCA CAGAGCAAGC GAGGGCCCGG 
GGACACCAGC TGGAGGAGCT GCGGAAGCTT CGGACATTTT TGCAGGATTC CAATGAGGTA 
ACTGCATGGC TGAGGGAGAA GAGCCTGGCG GCTCTGGATG AGGGCCAGCA GGACCCAGCC 
ACGATGCAAA CACAGTTGCA GAAGCAGCAG AATTTCCAGG CTGAGCTGGA CGCCAGTGTT 
CACCAACAGC AGGAGGTCCA GATGGAGGGG CAGAAGCTGC TACAAGGGGG CCACCCGGCC 
TCAGAGACCA TCCGGGGGCA ACTGGAAGAA TTAGGAGGCC TCTGGGATGA GCTGCAGACC 
AACTGCCAGA GGAAGATGGC CAGACTACAG GGGGCCCTCA AGGTCCTGCA TCTGCAGAGA 
ATGCTGAAGG AACTGGAGAA GTGGCTGGAG CACATGGAAG CAGAGCTGAG AGTCCCTGTC 
AGAAGCCAGG CGCTGCCTAG AGTGGGCGAA CTCCTGGGAG CCCAGGAGGA GCTGGAAGCA 
GCCATGGACA GGCAGGCCAA GGAAGTGCAA GAGTTGCAGG GCCAGTCCCA AGCCTGCCTC 
CAGGAAGGCC ACTGCCTCGC CAAAGATGTG GAAGAGCAAG CCCGGCAGCT GCTTCAGAGG 
TTTCAGAGCC TTCGGGAGCC CCTACAAGAG CGCAGGGCAT CCCTGGAAGC CCAAAGGCTC 
CTCTTACAGT TCTTCAGGGA TGCTGATGAG GAAATGGCCT GGGTGCAGGA GAAGCTGCCC 
TCAGCCACAG CCCAGGACTA CGGCCAGAGC CTTAACACTG TGCGCCACCT GCAGGAGAAA 
CACCAGAATT TGGAGAATGA GATCCATAGC CACAAGGCTC TGAGCCAGGT GGTGACAGGT 
ACAGGGCACA AGCTCATACA GGCAGGGCAC TTTGCCACTG AAGAGGTGGC CGCCCGGGTG 
CAGCAGCTGG AGGTGGCTCT AAACCGCTTA GAGACAGAGG CCGCACAGAG GAGGAGGAGA 
CTGCAGCAGG CCCTGGAGGC CCAGCAGACT TTGGTGGAGC TCCTGGAAGC AGGATCCTGG 
CTGGCTGAAC GGGACCATAT TCTGGACAGT GAGGACCTGG GCCAGGACGC TGAGGCCACA 
CAGGCTCTTC TGCGGTGCCT AGAGGCCACC ACAAGAGACT TGGAAGGGTT CAGCAGTCGC 
ATTGAGCAGT TACAGCAAAC AGTTGCCCTT CTGGAGAGTG GACAGACCCC AGGCAGTCCT 
AGGGTGCTGG CCCAGCTGCA GGCTGTGAGA GAGGCCCATG CACGGCTGCT GCAGAGAGCA 
GAAAGCAGGG GGGAGGCGCT GCGTGAACAG CTGCACCTGT ACCAGCTGGA GCAAGAGGCC 
TTGCTCCTGG ATGCCTGGCT GACCACCAAG CTGGCCGTCG CTGAGTCTCA GGACTATGGA 
CAGGATCTAG CAGGCATCAA GGTGCTGGAA GACATGTTTG GTGCTTTCAA CAGAGAAGTC 
CAGAGCCTGG GCCAGGCCAA GATGCAGACC CTGAGGGAGC GGATGGCTTC CCTAGAAAGA 
GGAGCACCCA GGTTCTACCC CCAGATTCAA GCTCAGAAGT GTCGCGTCCA GGCCGCTTGG 
GAGGGGCTGA ACAAGGCAAT CAAAGTCCGT ACAGAGAACC TGGCAGCAGC CTGCGATCTC 
CGGAGCTTTG AGCAGGCTGC CTCGGAGCTG CAGAGATGGA TACAGGAGAA GACCACCCTG 
CTGGAGGAAG CATTCCAAGT CCACAGCCTG TCACCTTCAC AGCCCCTTCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC ATAGGCGCCT GCAGAGAGAA CTGAGGGCTA TTGAAAAAGA GGTGTCAAGA 
GTGCAGATGG AGGCCCATCG GCTCGGCCAG CACTACCCTG TGGCTCAGGG GAGTCTTAGT 
GAGTGGCTCA CCAAGGTGCA GGGGGCCTGG ACCAACCTGG AGGCCAAAGT CCAGGAATGG 
AGCCAGAAGC TGTTGCAGGC TACCCAGGGC CACACCTTTC TTGGGAGCTG CCGGGAATTG 
CTAGCGTGGG CTCAGGAGAT GCAGGAGCTG CTTTCTAAGG AGAAGCAGGC TGGGGATGTG 
GTGGGGGCCA AGCAGTTCCT TGAGCAGCAT GAGGCGCTGG AGCAAGAGAT TCAGGAACGC 
TGCCTTCAAG CCCAGACCAT ACGGCACGAA GGGCAGCAGC TGCTGGACAA CGGCCACTTC 
CTGTCCCCAG AGGTGGCAGA GTGTATGCAG GAGCTGGAAA GACATCTGCA GGAGCTTCAG 
GTAGCCTGGG CCCTGCGTGG ACAACGCTGG GAACAGACCC GGAGCCTGCA ACAGCTGCGG 
CAGAGGCTGG AGCTGGCTGA GGCTTGGCTG GCCTCCTGGG AGCGCCTGCT GCTGGACCCC 
AGCTGTGGGC ACTCGGTGTT GGAAGTGGAA CGCCTGCTCT ACAGACACGA GGGCTTAGAA 
AAGCTGCTGG TGGCCCACGA GGAGACATTC ATTCAGCTTC AGACGATGAC AGAGGAGGCA 
AAGGGTGGTC ATGTCACGGA GGCATCTGTG GAACAGCAGC CCCCCACAGA AGGGAGGCAG 
CCCAGAACCA GCTTCCCAAC CAGCAGCCAT GGGATCTTGG CAAGGGGAGC CTCGAGCCCA 
TTCTCAGATA TGAGGAAGGC AGAAGTGCCA AGTGGCACTG GGGAACTGGC TTACGTGTCC 
CCCCTTGTAC CTGAGGAGAC TGAATGTGAG AGGCTGGAGG ACAGAAGGCA GACTTTCTTC 
CCAAGTCTGT TTGCAGTGTT GTCTGAAAAG CAGGCTGCAA GCTGGCAGCC AGCCTTGGGT 
AGCATGGCAG GTGGGCTGGA GGGGAAGGAA GGAGGGGCTG CTGGGGCCCA GGTTCCCAGT 
GTTATTGTAC CTTACAGAGC TATGAGCTTT TACAGCCCAT CTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500600.1
CDS	3252..14519
Translation	MGTVYQDRASDSWQKDHQFPQYFPAPMPPPPPDTGKCPVAIHPQRAKAHKLVVPSTLHLLSRSVPDEKTQAQSSALLSSSLAMDSEYEIGHVRKLQAQHTHMQEKTFTNWINNIFRLGRVGIRIQNLYTEFADGAHLLRLLELISGEALPAPSPGRLRVHFLENNSHALAFLRAKVPIPFIGPENIVDGDQSLILGLLWVIILRFQISHISLDREEFGASAALLSAKEALLVWCQRKTAGYTNVDITDFSRSWSDGLGFNALLHAHRPDLLDYGSLSPDRPLYNLSFAFRVAEQQLGIAQLLDPEDVAALHPDECSIMTYLSQYYHYFSRLQRGHTAQRRLAKILLQLQETEVLQAQYKQLVADLLCWIEEKKMQLEARDFPDSLPAMRQLLAAFASFRAREKPPRWQQRGATEALLFQLQTTLRAQNRRPFLPREGLGPAELAQRWAELERAEACRSQAMQQRLLQLERLDTLARRFQCKASHRESFLNDVEQMLDQARASLTDPATVEAATQRLSVLEAAILPQEGRFQALGEMADILRQEEYHSWADMARRQKEITGRWRRLLRCLQEERKRMEDSKAVLSLLQGVEAVTHQLGELQVLASSTVYGQQLAEIVSLLQSHDLLEAQVSAHRTHVTHLVHQTTQLDSQGTSVEVLQAKALALAELHHSLVSLVRARRTLLEQTLQRAQFLRSCEEEEAWLQEHRQLMETAVLDRDLTQIATALQKHKALETELHRHQAVCVDLMQRGRNFSVREPLTQPDPLERAEAVQGTWQLLWAGAARRRARLQTALLIGQYFSDSAEAASWLFQRQKQLESASCGKDQADAEALLLQHLRLEQDVRAFAAELRELEEQARAAAALVSHMVHTGTWKGSRLVSPTPNEEPPEDWKRLGRDTIGGPQGETQVLAAPGSHYSHQQIAFFSREEAPNTWKLEELGDSQSRMAIIHSIEKKPQVLSALGFLGEGPRTVLQAENDVHFNSSTILQAQADVSQTYEKLRALAKLHRTRLEESIALFSFYSSCRELQSWLEKQTALFQTLQPQGHNLEVIQLKYENVLMTLATGKGHWAEAINTAEQLKQRCPGHISKIQQQQEDLKQRWQQLEALKEEKLLQLTRGMEVCSLLQESEPIQAQLLNVISRLETLGTRSSGDSHHTLQQTQQKVLVLEKKISYLQRATIEVMESGSPESRLLWEQVLMLQSLLKQAQGQVARQIQVQTVARVQRGILQESQKLLLWAEDIQAQLCSKEERQVEASALRPLRRHGTLQEETCLWEERLQQLEAQGQPVAVSDSPQSQEVASALRLLGQHSQQLKALWEQRQQKFWEGLELQGFGQEVDDFMATCASHEALLQLDNLGEDIREAQSLLQQHQGLGWLRSTLGSRAEALRARGEKLLLSHPAAVSKIRELLHSAQAQWTRVQERSEQRRVQLLASLQLQEWKQAEEGLMLWMEEKWPRVADERSQAGSNILQKLKWRKITKSELLASRRYMEELQQAGKELLHSNPYAQEDIQDRLQSLNHKWEELNHKMEDRGDRLPQTRQQDQLLELIQDVQETMEHLEGALQSTETGQDLCSSRRLQRQHCKLEDKSQALASKMDALISQTHNAFTSWTILEESQKCHQRFKSLQSKLATQHQQLQASVELYEFNLLSNLELTWAAEHMPNAALNCPAQCWHDAHSLQRKHKMLQAEVKGHVRHMYRVLSSGQRLAASGHPRAQHIVEQCQKLESHWAGLEQACEERAHCLQQAVTVQQYFLNVSEMETWVEEKRPLASSQDYGSNEEATSGLIRKHQMLQQEVALYWSSMEDLEQRFQTLAEFEAPERLGVVREKLQALRKLADERGQELEGTLRLHEFMREAEDLQSWLSSRKQVARGGDTFGEDHEHVLQLCTEFTKFQYQVETGAQRVETCRLLAESLQERGHSAAPKAHQRQQDIQASWSELCQLTQARSRLLNDAEITLRVHGDLLEVLTQIQEKATSLPNDVAQDLCGVENQLQRHERLERELAGMEQQVQELMKAGGRVQELCPGTQALAAVQQKQQAVTQAWEALQLRMEQRKAQLERGYLLVRFHTAVRDYTSWAASVHQELQMEEASWEPHSLLLRLRAHQWLWAELKAKEELQQRATKMGQQALLAAGTPAKVQDGLRTLQEERDQVFQAWALKQEKLQAMLQEQHLLRQCGHLVEVLTAQEAFLKASGLGSSVEEVEQLIRKHVIFQKVLALQDKKESALNEQLKTISSPKGQNLFCHMLEHRAQVKELAESRGQALHTSLMIGNFVRAATQAEDWIQQRVQQLRAWSPLGNLKDYLKHLRKHQAFRAEVQAQEQILTSVAKQGEELLSQSHPQAGEVSQRLEALRDLWEKLRQAVTLQGQALENRYNFQEFLQRVDLAETWIQEKERMVNSCDIGLNLEHCLQLCRQVRRLQVTVDDAHIKGIKNLSLQLKNQGPEESETICQRQNQLNNRWKTFHGNLLLYQQRLEAALEIHRLSRELDDVIERLREKESLIWAPEGTEDLENVQRLSWRQKVLQQEMGLIQTQVESLEGRIGRLCKESPEVAHSLRHKQQEMMDSWWKVWSKAQNRRELLDVGHEVQKLQTLLQDLQDWARGLQAEMATQGTPCSPVRIQYMLEEYRAYKVELDIRTESLNLVQSMGQRLLASGYPQASEIHQTLAAVEQGLSSLRESWQGRQQQLQQALEQQLFLGSVEKAERWLDSEEASLASEGVADPLVTVETLLSKLKRHEQGLKAQAEKIRALEATAHSLHQGGHSEARSILDRCQALLLRTEALTEQARARGHQLEELRKLRTFLQDSNEVTAWLREKSLAALDEGQQDPATMQTQLQKQQNFQAELDASVHQQQEVQMEGQKLLQGGHPASETIRGQLEELGGLWDELQTNCQRKMARLQGALKVLHLQRMLKELEKWLEHMEAELRVPVRSQALPRVGELLGAQEELEAAMDRQAKEVQELQGQSQACLQEGHCLAKDVEEQARQLLQRFQSLREPLQERRASLEAQRLLLQFFRDADEEMAWVQEKLPSATAQDYGQSLNTVRHLQEKHQNLENEIHSHKALSQVVTGTGHKLIQAGHFATEEVAARVQQLEVALNRLETEAAQRRRRLQQALEAQQTLVELLEAGSWLAERDHILDSEDLGQDAEATQALLRCLEATTRDLEGFSSRIEQLQQTVALLESGQTPGSPRVLAQLQAVREAHARLLQRAESRGEALREQLHLYQLEQEALLLDAWLTTKLAVAESQDYGQDLAGIKVLEDMFGAFNREVQSLGQAKMQTLRERMASLERGAPRFYPQIQAQKCRVQAAWEGLNKAIKVRTENLAAACDLRSFEQAASELQRWIQEKTTLLEEAFQVHSLSPSQPLLQQQQQQQQQQQQQQQQQQQQQQQQQQHRRLQRELRAIEKEVSRVQMEAHRLGQHYPVAQGSLSEWLTKVQGAWTNLEAKVQEWSQKLLQATQGHTFLGSCRELLAWAQEMQELLSKEKQAGDVVGAKQFLEQHEALEQEIQERCLQAQTIRHEGQQLLDNGHFLSPEVAECMQELERHLQELQVAWALRGQRWEQTRSLQQLRQRLELAEAWLASWERLLLDPSCGHSVLEVERLLYRHEGLEKLLVAHEETFIQLQTMTEEAKGGHVTEASVEQQPPTEGRQPRTSFPTSSHGILARGASSPFSDMRKAEVPSGTGELAYVSPLVPEETECERLEDRRQTFFPSLFAVLSEKQAASWQPALGSMAGGLEGKEGGAAGAQVPSVIVPYRAMSFYSPSL

Target ORF information:

RefSeq Version	XM_006500537.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500537.2

ORF Insert Sequence:

ATGGGGACAG TGTATCAAGA TAGGGCCTCA GACTCCTGGC AGAAGGATCA TCAGTTTCCA 
CAGTACTTCC CAGCCCCCAT GCCTCCACCA CCTCCAGACA CAGGGAAATG CCCAGTCGCC 
ATTCACCCTC AAAGAGCTAA GGCTCATAAG CTGGTTGTCC CATCAACCCT CCACCTCCTT 
AGCCGCAGCG TCCCTGACGA GAAGACCCAG GCCCAAAGCT CAGCCCTTCT GAGTTCAAGT 
TTGGCCATGG ACTCTGAGTA TGAGATAGGC CATGTCCGCA AGCTCCAGGC TCAGCATACA 
CACATGCAGG AGAAGACCTT CACCAACTGG ATCAATAACA TCTTCCGACT AGGACGGGTG 
GGCATCAGGA TCCAGAACCT GTACACAGAG TTTGCTGACG GTGCCCACCT ACTGCGGCTA 
TTGGAGCTTA TCTCTGGGGA GGCCCTACCA GCGCCCAGTC CGGGCCGCCT GCGCGTGCAC 
TTCCTGGAGA ACAACAGCCA CGCCCTGGCC TTCCTCAGGG CCAAGGTGCC CATTCCCTTC 
ATCGGGCCAG AGAACATTGT GGATGGGGAC CAGTCACTCA TCCTGGGACT CCTCTGGGTC 
ATCATTCTGC GTTTCCAGAT TTCCCACATC TCCTTGGACA GGGAGGAGTT TGGGGCCAGT 
GCGGCTTTGC TGTCTGCCAA GGAAGCCCTG CTGGTCTGGT GTCAGCGGAA GACAGCCGGC 
TACACCAATG TGGACATCAC TGACTTCTCC CGAAGCTGGA GTGACGGGCT CGGCTTTAAT 
GCCCTGCTCC ACGCTCACAG GCCGGACCTG CTGGATTATG GCTCGCTGAG TCCAGACCGC 
CCTCTGTATA ACCTCTCCTT TGCCTTCCGA GTGGCTGAGC AGCAGCTTGG CATTGCCCAG 
CTGCTCGACC CTGAGGATGT GGCAGCCCTG CATCCAGATG AGTGCTCCAT CATGACCTAC 
CTGTCTCAGT ACTACCACTA CTTCTCCCGT CTGCAGCGGG GGCACACGGC CCAGAGGAGA 
CTGGCTAAGA TCCTGCTGCA GCTGCAGGAG ACGGAGGTAT TGCAGGCACA ATACAAGCAA 
CTGGTGGCTG ACCTGCTCTG CTGGATTGAG GAGAAGAAGA TGCAGCTAGA AGCTCGGGAC 
TTCCCAGACT CACTGCCTGC CATGCGCCAG CTACTGGCAG CCTTTGCCTC CTTCCGTGCC 
CGGGAAAAGC CACCTCGGTG GCAGCAGCGA GGAGCCACAG AGGCCCTGCT CTTCCAGCTG 
CAGACAACAC TCCGAGCCCA AAACCGAAGG CCATTCCTAC CTCGAGAGGG CCTGGGCCCT 
GCAGAGCTGG CCCAGCGCTG GGCAGAACTG GAGAGGGCAG AGGCCTGCAG GAGCCAGGCC 
ATGCAGCAAA GGCTGCTACA GCTGGAGCGA CTGGACACTC TGGCCCGCCG CTTTCAGTGC 
AAGGCATCCC ACCGGGAAAG CTTTTTAAAT GACGTAGAGC AGATGTTAGA CCAGGCCAGA 
GCCTCCCTCA CCGACCCAGC CACAGTGGAG GCAGCCACTC AGAGGCTGAG CGTTCTGGAG 
GCTGCCATCC TGCCCCAGGA AGGGCGCTTC CAGGCCTTGG GTGAGATGGC AGACATCCTC 
CGGCAGGAAG AGTATCACAG CTGGGCGGAT ATGGCCCGCA GGCAGAAGGA GATCACGGGG 
CGCTGGCGGA GGCTCCTACG GTGTCTACAG GAGGAGAGGA AGCGCATGGA AGACTCAAAG 
GCTGTGCTGA GTCTGCTGCA GGGGGTGGAG GCTGTCACTC ACCAGCTTGG GGAGCTGCAG 
GTGTTGGCCA GCTCCACAGT CTATGGGCAA CAGCTGGCAG AAATAGTGTC GCTGCTGCAG 
AGCCATGACC TGCTGGAGGC CCAGGTGTCA GCCCACAGGA CCCATGTGAC TCATCTTGTC 
CACCAGACCA CCCAGCTAGA CTCCCAGGGT ACCAGTGTGG AGGTGCTGCA GGCTAAAGCC 
CTAGCACTGG CCGAGCTCCA CCACAGCCTG GTGTCTCTTG TCAGAGCCCG ACGTACCCTG 
CTGGAGCAGA CCCTCCAGCG AGCACAGTTT CTGCGCAGCT GTGAGGAGGA GGAGGCCTGG 
TTGCAAGAGC ACAGACAGCT AATGGAGACC GCGGTCCTGG ACCGGGACCT CACTCAGATC 
GCTACTGCTC TGCAGAAACA CAAGGCTCTG GAAACAGAAC TCCATCGCCA CCAGGCAGTG 
TGTGTAGATC TCATGCAGAG AGGACGCAAC TTCAGTGTCA GAGAGCCCCT GACACAGCCA 
GATCCACTGG AAAGGGCAGA AGCAGTGCAG GGAACCTGGC AGCTGCTCTG GGCTGGAGCC 
GCAAGGCGGC GCGCCCGGCT GCAAACAGCA CTGCTAATTG GGCAGTACTT TTCTGACTCG 
GCCGAAGCAG CTTCCTGGCT TTTCCAGCGG CAGAAACAAC TAGAGAGCGC ATCCTGCGGG 
AAGGACCAGG CAGACGCCGA GGCCCTGTTG CTGCAACACC TGCGCCTGGA GCAAGACGTG 
CGCGCCTTCG CGGCGGAGCT GCGCGAGCTA GAGGAGCAGG CGCGGGCTGC CGCAGCCCTA 
GTGTCACACA TGGTGCACAC GGGGACATGG AAAGGATCCA GACTTGTCTC TCCTACACCA 
AATGAGGAAC CTCCAGAAGA CTGGAAAAGG CTTGGCAGAG ACACCATTGG AGGACCCCAG 
GGGGAGACTC AAGTTTTAGC AGCACCAGGC AGCCATTATT CACATCAGCA GATAGCCTTC 
TTCTCCAGAG AAGAGGCTCC AAATACCTGG AAGCTTGAAG AGTTGGGAGA CAGTCAGAGC 
AGGATGGCCA TCATCCACAG CATCGAGAAG AAACCCCAGG TGCTATCTGC CCTGGGCTTT 
CTAGGAGAAG GCCCGAGGAC AGTTCTGCAG GCTGAGAATG ACGTTCACTT CAACTCCAGC 
ACCATACTCC AGGCTCAGGC TGACGTGAGC CAGACCTATG AGAAGCTTAG GGCCCTGGCA 
AAGCTTCACA GGACCCGACT GGAGGAGTCA ATCGCCCTGT TTAGCTTTTA TAGCTCCTGT 
AGGGAGCTTC AGTCCTGGCT GGAGAAGCAG ACCGCACTGT TCCAAACATT GCAGCCCCAG 
GGACACAACT TAGAGGTCAT ACAACTCAAA TATGAGAATG TTCTCATGAC CTTGGCCACT 
GGAAAAGGCC ACTGGGCAGA GGCCATCAAC ACTGCTGAAC AACTGAAGCA GAGATGTCCA 
GGACACATCT CGAAAATCCA ACAACAACAG GAGGACCTGA AGCAAAGGTG GCAGCAGCTG 
GAAGCCCTGA AGGAGGAGAA ACTCCTGCAG CTGACACGTG GCATGGAGGT GTGCAGTCTT 
TTACAGGAGT CTGAACCCAT ACAAGCCCAA CTGCTAAATG TGATAAGCAG GCTGGAGACT 
CTGGGAACAA GAAGCTCTGG GGACAGCCAT CATACCCTAC AGCAGACCCA GCAGAAGGTC 
CTGGTATTGG AGAAGAAGAT CTCCTACCTC CAAAGAGCAA CCATAGAGGT GATGGAGTCA 
GGCTCCCCAG AGAGCAGGCT GCTTTGGGAG CAGGTCCTGA TGCTGCAGTC ACTGCTAAAG 
CAAGCGCAAG GGCAGGTGGC CCGGCAGATC CAGGTCCAGA CTGTGGCTCG GGTTCAGCGA 
GGCATCCTCC AGGAGAGCCA GAAGCTGCTG CTGTGGGCAG AGGACATCCA GGCTCAGCTG 
TGCAGCAAAG AGGAGCGGCA GGTTGAGGCT TCAGCCCTGC GGCCGCTGAG GAGGCATGGA 
ACCCTACAGG AGGAGACCTG CCTGTGGGAA GAGAGGCTGC AGCAGCTGGA GGCTCAGGGT 
CAGCCGGTGG CAGTCTCAGA CTCTCCACAG TCCCAGGAGG TGGCCAGCGC TCTAAGGCTC 
CTAGGCCAGC ACAGCCAGCA GCTGAAGGCT CTGTGGGAGC AGAGACAGCA GAAGTTTTGG 
GAGGGGCTGG AGCTGCAGGG GTTTGGCCAA GAAGTAGATG ATTTCATGGC CACCTGTGCC 
AGCCATGAGG CCTTACTGCA GCTGGACAAC CTTGGGGAGG ACATAAGGGA GGCCCAGAGC 
CTGCTGCAGC AGCACCAGGG TTTGGGGTGG CTCCGCAGCA CCCTGGGATC ACGGGCAGAG 
GCCCTCCGTG CACGCGGTGA GAAGCTGCTT CTGAGCCATC CAGCTGCCGT GAGCAAGATC 
AGAGAGCTGC TGCACAGTGC CCAGGCCCAG TGGACCAGGG TCCAAGAGAG GAGTGAGCAG 
AGAAGGGTCC AGCTGCTGGC TTCCCTCCAA TTGCAGGAAT GGAAGCAGGC TGAAGAAGGC 
CTAATGCTGT GGATGGAGGA GAAGTGGCCC AGGGTGGCTG ATGAGCGCTC CCAAGCAGGA 
AGCAACATTC TGCAGAAACT CAAGTGGCGC AAAATAACTA AGAGTGAGCT ATTAGCCTCT 
CGGAGGTACA TGGAAGAGCT ACAGCAGGCT GGGAAAGAAC TGTTGCATAG CAACCCATAT 
GCACAGGAGG ACATACAGGA CAGACTTCAA AGCCTTAATC ACAAATGGGA AGAGTTGAAC 
CACAAGATGG AAGATCGAGG GGATAGGCTG CCTCAGACCA GACAGCAGGA CCAGCTCCTA 
GAACTAATAC AGGATGTCCA GGAGACAATG GAGCATCTGG AGGGAGCCCT GCAGAGCACA 
GAAACCGGGC AGGACCTGTG CTCCAGCCGG AGGCTGCAGA GACAGCATTG CAAGCTAGAG 
GACAAGAGCC AGGCACTGGC CAGCAAGATG GATGCCCTCA TCTCTCAGAC CCACAATGCA 
TTCACTTCCT GGACCATCCT GGAGGAGAGC CAGAAGTGTC ACCAGAGGTT CAAGTCCCTG 
CAGAGCAAGC TAGCCACCCA ACACCAGCAA CTGCAGGCCT CAGTTGAGCT GTATGAATTT 
AATCTCCTTA GCAACCTGGA GCTCACATGG GCAGCTGAGC ACATGCCCAA TGCCGCTCTC 
AACTGCCCTG CCCAGTGCTG GCATGATGCC CACAGCCTTC AACGAAAGCA CAAGATGCTC 
CAGGCTGAGG TGAAAGGTCA TGTAAGGCAC ATGTACAGGG TGCTAAGTTC TGGTCAGCGT 
CTAGCAGCCT CTGGGCACCC TCGAGCTCAG CATATCGTGG AACAGTGCCA GAAGCTAGAA 
AGCCACTGGG CAGGACTGGA GCAGGCGTGT GAAGAACGTG CACACTGCCT GCAACAGGCT 
GTCACTGTCC AGCAGTACTT TCTGAACGTG TCAGAGATGG AGACCTGGGT GGAGGAAAAG 
CGGCCTCTGG CAAGCAGTCA GGACTACGGC AGTAATGAGG AAGCTACCAG TGGGCTCATT 
AGGAAACACC AGATGCTCCA GCAGGAGGTA GCTCTTTATT GGAGCTCCAT GGAAGATCTC 
GAGCAGAGGT TCCAGACCCT TGCTGAATTT GAGGCCCCTG AGCGGTTGGG TGTGGTACGG 
GAGAAGTTGC AGGCACTACG GAAGCTGGCA GACGAACGGG GGCAGGAGCT GGAGGGGACC 
CTGAGACTAC ATGAGTTCAT GAGAGAAGCT GAGGACCTGC AGAGCTGGCT GTCCAGCAGG 
AAGCAGGTGG CCAGAGGAGG GGACACCTTT GGAGAGGACC ATGAGCACGT GCTGCAACTC 
TGCACTGAGT TTACAAAGTT TCAGTACCAG GTTGAAACTG GTGCCCAGCG AGTGGAAACC 
TGTCGACTGC TGGCAGAGAG CCTACAAGAG CGTGGGCACA GTGCTGCCCC CAAGGCTCAC 
CAGAGGCAGC AGGATATACA GGCTTCCTGG TCTGAACTGT GCCAGTTGAC CCAGGCCCGA 
AGCCGTCTGC TCAATGACGC CGAAATCACT CTTAGAGTCC ACGGAGACCT TCTGGAAGTC 
CTTACCCAGA TTCAGGAGAA AGCCACAAGC CTCCCTAACG ATGTGGCCCA GGACCTGTGT 
GGGGTAGAGA ACCAGCTCCA GAGACATGAG AGGCTGGAAC GCGAGTTGGC AGGCATGGAG 
CAGCAGGTGC AGGAACTGAT GAAGGCTGGA GGCAGAGTGC AGGAGCTGTG TCCAGGAACT 
CAGGCCCTTG CCGCTGTCCA GCAAAAGCAG CAAGCTGTGA CCCAAGCTTG GGAGGCCCTC 
CAGCTGCGCA TGGAGCAGCG CAAGGCTCAA TTGGAACGAG GATATCTCCT GGTCCGGTTC 
CACACAGCGG TGAGGGACTA TACCTCCTGG GCGGCCAGCG TGCATCAGGA GCTACAGATG 
GAGGAGGCCT CCTGGGAACC CCACAGCCTT TTGCTCAGGC TCAGAGCCCA TCAATGGCTG 
TGGGCAGAAC TGAAAGCCAA GGAGGAGCTG CAGCAACGGG CCACTAAGAT GGGCCAGCAA 
GCTCTGCTGG CAGCAGGAAC ACCCGCTAAG GTCCAGGATG GGCTTCGAAC CCTACAGGAG 
GAACGCGACC AAGTGTTCCA GGCCTGGGCA CTCAAGCAAG AGAAGCTGCA GGCCATGCTT 
CAAGAACAGC ACCTCCTCAG GCAATGCGGC CACCTAGTGG AGGTCCTCAC AGCCCAGGAG 
GCATTCCTAA AAGCCAGTGG CCTGGGGAGC TCAGTGGAAG AAGTGGAGCA GTTGATCCGA 
AAGCACGTGA TCTTCCAGAA AGTGCTAGCT CTCCAGGACA AGAAGGAGTC GGCTCTGAAT 
GAGCAGCTAA AGACGATTTC AAGCCCTAAG GGGCAGAACC TGTTTTGCCA CATGTTGGAG 
CACCGGGCTC AAGTGAAGGA ACTGGCAGAG AGCCGGGGCC AGGCTCTGCA CACCTCCCTG 
ATGATAGGCA ACTTTGTCAG GGCAGCAACC CAGGCTGAGG ACTGGATCCA GCAGCGGGTC 
CAGCAACTGA GAGCCTGGAG CCCTCTTGGG AATCTAAAGG ATTATCTGAA GCACCTGAGG 
AAACACCAGG CCTTCAGGGC TGAGGTCCAG GCTCAGGAGC AGATCTTGAC CTCTGTTGCC 
AAGCAAGGCG AAGAGCTCCT CAGTCAGAGC CACCCTCAAG CTGGAGAGGT CTCCCAGAGG 
CTGGAGGCCC TGCGGGACCT CTGGGAGAAG CTGAGGCAGG CAGTGACCCT GCAGGGCCAG 
GCCCTAGAGA ACAGATACAA CTTCCAGGAG TTCCTGCAGA GAGTGGACCT AGCAGAGACC 
TGGATCCAGG AGAAGGAGAG GATGGTGAAC AGCTGTGACA TTGGCCTGAA CCTTGAGCAC 
TGCCTGCAGC TCTGCAGACA AGTCCGCAGG TTACAGGTCA CAGTGGATGA TGCCCACATC 
AAGGGAATCA AAAACTTGTC ACTGCAGCTC AAGAACCAGG GCCCTGAGGA ATCCGAGACC 
ATCTGCCAGC GGCAAAACCA GCTCAACAAT AGGTGGAAGA CTTTCCATGG CAACCTGCTC 
CTCTATCAGC AGCGGCTTGA AGCAGCCCTG GAGATACATA GATTGTCCAG GGAACTGGAT 
GACGTTATTG AGCGGCTCAG GGAGAAGGAA TCCCTGATCT GGGCCCCAGA GGGCACTGAA 
GATTTGGAGA ATGTGCAGAG GCTATCATGG AGACAGAAAG TGCTGCAGCA GGAGATGGGC 
CTCATCCAGA CCCAGGTGGA GTCTCTTGAA GGTAGGATTG GCCGGCTCTG CAAGGAAAGT 
CCCGAGGTTG CCCACAGCCT CAGACATAAG CAGCAAGAAA TGATGGACAG CTGGTGGAAG 
GTCTGGAGCA AAGCCCAGAA CAGGAGGGAG TTGCTGGATG TGGGCCATGA AGTTCAGAAA 
CTCCAGACCC TGCTGCAGGA TCTGCAGGAC TGGGCCCGGG GACTACAGGC TGAGATGGCC 
ACACAGGGCA CCCCCTGCAG CCCTGTGAGA ATCCAGTACA TGTTGGAAGA GTACCGGGCA 
TACAAGGTTG AGCTGGACAT CCGAACAGAG AGCCTTAACC TGGTCCAAAG CATGGGGCAG 
CGGCTGCTTG CATCTGGCTA CCCACAGGCC TCTGAGATTC ACCAGACCCT GGCCGCTGTA 
GAGCAGGGGT TGAGTAGCTT GCGGGAATCC TGGCAGGGCC GGCAACAGCA GCTACAGCAG 
GCCTTGGAGC AGCAGCTGTT TCTGGGCTCT GTGGAAAAGG CGGAACGCTG GCTTGACAGT 
GAAGAGGCCT CCCTGGCTAG TGAGGGAGTG GCGGACCCCT TGGTCACTGT GGAGACCCTT 
CTGTCAAAGC TCAAGAGGCA TGAGCAGGGC CTGAAGGCCC AGGCCGAAAA GATCCGTGCA 
TTGGAGGCCA CAGCCCACAG CCTGCACCAG GGTGGACACT CTGAGGCGCG TAGCATCCTG 
GACAGATGCC AGGCCCTGCT CCTGAGGACA GAGGCACTCA CAGAGCAAGC GAGGGCCCGG 
GGACACCAGC TGGAGGAGCT GCGGAAGCTT CGGACATTTT TGCAGGATTC CAATGAGGTA 
ACTGCATGGC TGAGGGAGAA GAGCCTGGCG GCTCTGGATG AGGGCCAGCA GGACCCAGCC 
ACGATGCAAA CACAGTTGCA GAAGCAGCAG AATTTCCAGG CTGAGCTGGA CGCCAGTGTT 
CACCAACAGC AGGAGGTCCA GATGGAGGGG CAGAAGCTGC TACAAGGGGG CCACCCGGCC 
TCAGAGACCA TCCGGGGGCA ACTGGAAGAA TTAGGAGGCC TCTGGGATGA GCTGCAGACC 
AACTGCCAGA GGAAGATGGC CAGACTACAG GGGGCCCTCA AGGTCCTGCA TCTGCAGAGA 
ATGCTGAAGG AACTGGAGAA GTGGCTGGAG CACATGGAAG CAGAGCTGAG AGTCCCTGTC 
AGAAGCCAGG CGCTGCCTAG AGTGGGCGAA CTCCTGGGAG CCCAGGAGGA GCTGGAAGCA 
GCCATGGACA GGCAGGCCAA GGAAGTGCAA GAGTTGCAGG GCCAGTCCCA AGCCTGCCTC 
CAGGAAGGCC ACTGCCTCGC CAAAGATGTG GAAGAGCAAG CCCGGCAGCT GCTTCAGAGG 
TTTCAGAGCC TTCGGGAGCC CCTACAAGAG CGCAGGGCAT CCCTGGAAGC CCAAAGGCTC 
CTCTTACAGT TCTTCAGGGA TGCTGATGAG GAAATGGCCT GGGTGCAGGA GAAGCTGCCC 
TCAGCCACAG CCCAGGACTA CGGCCAGAGC CTTAACACTG TGCGCCACCT GCAGGAGAAA 
CACCAGAATT TGGAGAATGA GATCCATAGC CACAAGGCTC TGAGCCAGGT GGTGACAGGT 
ACAGGGCACA AGCTCATACA GGCAGGGCAC TTTGCCACTG AAGAGGTGGC CGCCCGGGTG 
CAGCAGCTGG AGGTGGCTCT AAACCGCTTA GAGACAGAGG CCGCACAGAG GAGGAGGAGA 
CTGCAGCAGG CCCTGGAGGC CCAGCAGACT TTGGTGGAGC TCCTGGAAGC AGGATCCTGG 
CTGGCTGAAC GGGACCATAT TCTGGACAGT GAGGACCTGG GCCAGGACGC TGAGGCCACA 
CAGGCTCTTC TGCGGTGCCT AGAGGCCACC ACAAGAGACT TGGAAGGGTT CAGCAGTCGC 
ATTGAGCAGT TACAGCAAAC AGTTGCCCTT CTGGAGAGTG GACAGACCCC AGGCAGTCCT 
AGGGTGCTGG CCCAGCTGCA GGCTGTGAGA GAGGCCCATG CACGGCTGCT GCAGAGAGCA 
GAAAGCAGGG GGGAGGCGCT GCGTGAACAG CTGCACCTGT ACCAGCTGGA GCAAGAGGCC 
TTGCTCCTGG ATGCCTGGCT GACCACCAAG CTGGCCGTCG CTGAGTCTCA GGACTATGGA 
CAGGATCTAG CAGGCATCAA GGTGCTGGAA GACATGTTTG GTGCTTTCAA CAGAGAAGTC 
CAGAGCCTGG GCCAGGCCAA GATGCAGACC CTGAGGGAGC GGATGGCTTC CCTAGAAAGA 
GGAGCACCCA GGTTCTACCC CCAGATTCAA GCTCAGAAGT GTCGCGTCCA GGCCGCTTGG 
GAGGGGCTGA ACAAGGCAAT CAAAGTCCGT ACAGAGAACC TGGCAGCAGC CTGCGATCTC 
CGGAGCTTTG AGCAGGCTGC CTCGGAGCTG CAGAGATGGA TACAGGAGAA GACCACCCTG 
CTGGAGGAAG CATTCCAAGT CCACAGCCTG TCACCTTCAC AGCCCCTTCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC ATAGGCGCCT GCAGAGAGAA CTGAGGGCTA TTGAAAAAGA GGTGTCAAGA 
GTGCAGATGG AGGCCCATCG GCTCGGCCAG CACTACCCTG TGGCTCAGGG GAGTCTTAGT 
GAGTGGCTCA CCAAGGTGCA GGGGGCCTGG ACCAACCTGG AGGCCAAAGT CCAGGAATGG 
AGCCAGAAGC TGTTGCAGGC TACCCAGGGC CACACCTTTC TTGGGAGCTG CCGGGAATTG 
CTAGCGTGGG CTCAGGAGAT GCAGGAGCTG CTTTCTAAGG AGAAGCAGGC TGGGGATGTG 
GTGGGGGCCA AGCAGTTCCT TGAGCAGCAT GAGGCGCTGG AGCAAGAGAT TCAGGAACGC 
TGCCTTCAAG CCCAGACCAT ACGGCACGAA GGGCAGCAGC TGCTGGACAA CGGCCACTTC 
CTGTCCCCAG AGGTGGCAGA GTGTATGCAG GAGCTGGAAA GACATCTGCA GGAGCTTCAG 
GTAGCCTGGG CCCTGCGTGG ACAACGCTGG GAACAGACCC GGAGCCTGCA ACAGCTGCGG 
CAGAGGCTGG AGCTGGCTGA GGCTTGGCTG GCCTCCTGGG AGCGCCTGCT GCTGGACCCC 
AGCTGTGGGC ACTCGGTGTT GGAAGTGGAA CGCCTGCTCT ACAGACACGA GGGCTTAGAA 
AAGCTGCTGG TGGCCCACGA GGAGACATTC ATTCAGCTTC AGACGATGAC AGAGGAGGCA 
AAGGGTGGTC ATGTCACGGA GGCATCTGTG GAACAGCAGC CCCCCACAGA AGGGAGGCAG 
CCCAGAACCA GCTTCCCAAC CAGCAGCCAT GGGATCTTGG CAAGGGGAGC CTCGAGCCCA 
TTCTCAGATA TGAGGAAGGC AGAAGTGCCA AGTGGCACTG GGGAACTGGC TTACGTGTCC 
CCCCTTGTAC CTGAGGAGAC TGAATGTGAG AGGCTGGAGG ACAGAAGGCA GACTTTCTTC 
CCAAGTCTGT TTGCAGTGTT GTCTGAAAAG CAGGCTGCAA GCTGGCAGCC AGCCTTGGGT 
AGCATGGCAG GTGGGCTGGA GGGGAAGGAA GGAGGGGCTG CTGGGGCCCA GGTTCCCAGT 
GTTATTGTAC CTTACAGAGC TATGAGCTTT TACAGCCCAT CTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn5 ( XM_030251931.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107791.1 Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), transcript variant X1, mRNA.

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CloneID	OMu87964
Clone ID Related Accession (Same CDS sequence)	XM_030251931.1
Accession Version	XM_030251931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACAG TGTATCAAGA TAGGGCCTCA GACTCCTGGC AGAAGGATCA TCAGTTTCCA 
CAGTACTTCC CAGCCCCCAT GCCTCCACCA CCTCCAGACA CAGGGAAATG CCCAGTCGCC 
ATTCACCCTC AAAGAGCTAA GGCTCATAAG CTGGTTGTCC CATCAACCCT CCACCTCCTT 
AGCCGCAGCG TCCCTGACGA GAAGACCCAG GCCCAAAGCT CAGCCCTTCT GAGTTCAAGT 
TTGGCCATGG ACTCTGAGTA TGAGATAGGC CATGTCCGCA AGCTCCAGGC TCAGCATACA 
CACATGCAGG AGAAGACCTT CACCAACTGG ATCAATAACA TCTTCCGACT AGGACGGGTG 
GGCATCAGGA TCCAGAACCT GTACACAGAG TTTGCTGACG GTGCCCACCT ACTGCGGCTA 
TTGGAGCTTA TCTCTGGGGA GGCCCTACCA GCGCCCAGTC CGGGCCGCCT GCGCGTGCAC 
TTCCTGGAGA ACAACAGCCA CGCCCTGGCC TTCCTCAGGG CCAAGGTGCC CATTCCCTTC 
ATCGGGCCAG AGAACATTGT GGATGGGGAC CAGTCACTCA TCCTGGGACT CCTCTGGGTC 
ATCATTCTGC GTTTCCAGAT TTCCCACATC TCCTTGGACA GGGAGGAGTT TGGGGCCAGT 
GCGGCTTTGC TGTCTGCCAA GGAAGCCCTG CTGGTCTGGT GTCAGCGGAA GACAGCCGGC 
TACACCAATG TGGACATCAC TGACTTCTCC CGAAGCTGGA GTGACGGGCT CGGCTTTAAT 
GCCCTGCTCC ACGCTCACAG GCCGGACCTG CTGGATTATG GCTCGCTGAG TCCAGACCGC 
CCTCTGTATA ACCTCTCCTT TGCCTTCCGA GTGGCTGAGC AGCAGCTTGG CATTGCCCAG 
CTGCTCGACC CTGAGGATGT GGCAGCCCTG CATCCAGATG AGTGCTCCAT CATGACCTAC 
CTGTCTCAGT ACTACCACTA CTTCTCCCGT CTGCAGCGGG GGCACACGGC CCAGAGGAGA 
CTGGCTAAGA TCCTGCTGCA GCTGCAGGAG ACGGAGGTAT TGCAGGCACA ATACAAGCAA 
CTGGTGGCTG ACCTGCTCTG CTGGATTGAG GAGAAGAAGA TGCAGCTAGA AGCTCGGGAC 
TTCCCAGACT CACTGCCTGC CATGCGCCAG CTACTGGCAG CCTTTGCCTC CTTCCGTGCC 
CGGGAAAAGC CACCTCGGTG GCAGCAGCGA GGAGCCACAG AGGCCCTGCT CTTCCAGCTG 
CAGACAACAC TCCGAGCCCA AAACCGAAGG CCATTCCTAC CTCGAGAGGG CCTGGGCCCT 
GCAGAGCTGG CCCAGCGCTG GGCAGAACTG GAGAGGGCAG AGGCCTGCAG GAGCCAGGCC 
ATGCAGCAAA GGCTGCTACA GCTGGAGCGA CTGGACACTC TGGCCCGCCG CTTTCAGTGC 
AAGGCATCCC ACCGGGAAAG CTTTTTAAAT GACGTAGAGC AGATGTTAGA CCAGGCCAGA 
GCCTCCCTCA CCGACCCAGC CACAGTGGAG GCAGCCACTC AGAGGCTGAG CGTTCTGGAG 
GCTGCCATCC TGCCCCAGGA AGGGCGCTTC CAGGCCTTGG GTGAGATGGC AGACATCCTC 
CGGCAGGAAG AGTATCACAG CTGGGCGGAT ATGGCCCGCA GGCAGAAGGA GATCACGGGG 
CGCTGGCGGA GGCTCCTACG GTGTCTACAG GAGGAGAGGA AGCGCATGGA AGACTCAAAG 
GCTGTGCTGA GTCTGCTGCA GGGGGTGGAG GCTGTCACTC ACCAGCTTGG GGAGCTGCAG 
GTGTTGGCCA GCTCCACAGT CTATGGGCAA CAGCTGGCAG AAATAGTGTC GCTGCTGCAG 
AGCCATGACC TGCTGGAGGC CCAGGTGTCA GCCCACAGGA CCCATGTGAC TCATCTTGTC 
CACCAGACCA CCCAGCTAGA CTCCCAGGGT ACCAGTGTGG AGGTGCTGCA GGCTAAAGCC 
CTAGCACTGG CCGAGCTCCA CCACAGCCTG GTGTCTCTTG TCAGAGCCCG ACGTACCCTG 
CTGGAGCAGA CCCTCCAGCG AGCACAGTTT CTGCGCAGCT GTGAGGAGGA GGAGGCCTGG 
TTGCAAGAGC ACAGACAGCT AATGGAGACC GCGGTCCTGG ACCGGGACCT CACTCAGATC 
GCTACTGCTC TGCAGAAACA CAAGGCTCTG GAAACAGAAC TCCATCGCCA CCAGGCAGTG 
TGTGTAGATC TCATGCAGAG AGGACGCAAC TTCAGTGTCA GAGAGCCCCT GACACAGCCA 
GATCCACTGG AAAGGGCAGA AGCAGTGCAG GGAACCTGGC AGCTGCTCTG GGCTGGAGCC 
GCAAGGCGGC GCGCCCGGCT GCAAACAGCA CTGCTAATTG GGCAGTACTT TTCTGACTCG 
GCCGAAGCAG CTTCCTGGCT TTTCCAGCGG CAGAAACAAC TAGAGAGCGC ATCCTGCGGG 
AAGGACCAGG CAGACGCCGA GGCCCTGTTG CTGCAACACC TGCGCCTGGA GCAAGACGTG 
CGCGCCTTCG CGGCGGAGCT GCGCGAGCTA GAGGAGCAGG CGCGGGCTGC CGCAGCCCTA 
GTGTCACACA TGGTGCACAC GGGGACATGG AAAGGATCCA GACTTGTCTC TCCTACACCA 
AATGAGGAAC CTCCAGAAGA CTGGAAAAGG CTTGGCAGAG ACACCATTGG AGGACCCCAG 
GGGGAGACTC AAGTTTTAGC AGCACCAGGC AGCCATTATT CACATCAGCA GATAGCCTTC 
TTCTCCAGAG AAGAGGCTCC AAATACCTGG AAGCTTGAAG AGTTGGGAGA CAGTCAGAGC 
AGGATGGCCA TCATCCACAG CATCGAGAAG AAACCCCAGG TGCTATCTGC CCTGGGCTTT 
CTAGGAGAAG GCCCGAGGAC AGTTCTGCAG GCTGAGAATG ACGTTCACTT CAACTCCAGC 
ACCATACTCC AGGCTCAGGC TGACGTGAGC CAGACCTATG AGAAGCTTAG GGCCCTGGCA 
AAGCTTCACA GGACCCGACT GGAGGAGTCA ATCGCCCTGT TTAGCTTTTA TAGCTCCTGT 
AGGGAGCTTC AGTCCTGGCT GGAGAAGCAG ACCGCACTGT TCCAAACATT GCAGCCCCAG 
GGACACAACT TAGAGGTCAT ACAACTCAAA TATGAGAATG TTCTCATGAC CTTGGCCACT 
GGAAAAGGCC ACTGGGCAGA GGCCATCAAC ACTGCTGAAC AACTGAAGCA GAGATGTCCA 
GGACACATCT CGAAAATCCA ACAACAACAG GAGGACCTGA AGCAAAGGTG GCAGCAGCTG 
GAAGCCCTGA AGGAGGAGAA ACTCCTGCAG CTGACACGTG GCATGGAGGT GTGCAGTCTT 
TTACAGGAGT CTGAACCCAT ACAAGCCCAA CTGCTAAATG TGATAAGCAG GCTGGAGACT 
CTGGGAACAA GAAGCTCTGG GGACAGCCAT CATACCCTAC AGCAGACCCA GCAGAAGGTC 
CTGGTATTGG AGAAGAAGAT CTCCTACCTC CAAAGAGCAA CCATAGAGGT GATGGAGTCA 
GGCTCCCCAG AGAGCAGGCT GCTTTGGGAG CAGGTCCTGA TGCTGCAGTC ACTGCTAAAG 
CAAGCGCAAG GGCAGGTGGC CCGGCAGATC CAGGTCCAGA CTGTGGCTCG GGTTCAGCGA 
GGCATCCTCC AGGAGAGCCA GAAGCTGCTG CTGTGGGCAG AGGACATCCA GGCTCAGCTG 
TGCAGCAAAG AGGAGCGGCA GGTTGAGGCT TCAGCCCTGC GGCCGCTGAG GAGGCATGGA 
ACCCTACAGG AGGAGACCTG CCTGTGGGAA GAGAGGCTGC AGCAGCTGGA GGCTCAGGGT 
CAGCCGGTGG CAGTCTCAGA CTCTCCACAG TCCCAGGAGG TGGCCAGCGC TCTAAGGCTC 
CTAGGCCAGC ACAGCCAGCA GCTGAAGGCT CTGTGGGAGC AGAGACAGCA GAAGTTTTGG 
GAGGGGCTGG AGCTGCAGGG GTTTGGCCAA GAAGTAGATG ATTTCATGGC CACCTGTGCC 
AGCCATGAGG CCTTACTGCA GCTGGACAAC CTTGGGGAGG ACATAAGGGA GGCCCAGAGC 
CTGCTGCAGC AGCACCAGGG TTTGGGGTGG CTCCGCAGCA CCCTGGGATC ACGGGCAGAG 
GCCCTCCGTG CACGCGGTGA GAAGCTGCTT CTGAGCCATC CAGCTGCCGT GAGCAAGATC 
AGAGAGCTGC TGCACAGTGC CCAGGCCCAG TGGACCAGGG TCCAAGAGAG GAGTGAGCAG 
AGAAGGGTCC AGCTGCTGGC TTCCCTCCAA TTGCAGGAAT GGAAGCAGGC TGAAGAAGGC 
CTAATGCTGT GGATGGAGGA GAAGTGGCCC AGGGTGGCTG ATGAGCGCTC CCAAGCAGGA 
AGCAACATTC TGCAGAAACT CAAGTGGCGC AAAATAACTA AGAGTGAGCT ATTAGCCTCT 
CGGAGGTACA TGGAAGAGCT ACAGCAGGCT GGGAAAGAAC TGTTGCATAG CAACCCATAT 
GCACAGGAGG ACATACAGGA CAGACTTCAA AGCCTTAATC ACAAATGGGA AGAGTTGAAC 
CACAAGATGG AAGATCGAGG GGATAGGCTG CCTCAGACCA GACAGCAGGA CCAGCTCCTA 
GAACTAATAC AGGATGTCCA GGAGACAATG GAGCATCTGG AGGGAGCCCT GCAGAGCACA 
GAAACCGGGC AGGACCTGTG CTCCAGCCGG AGGCTGCAGA GACAGCATTG CAAGCTAGAG 
GACAAGAGCC AGGCACTGGC CAGCAAGATG GATGCCCTCA TCTCTCAGAC CCACAATGCA 
TTCACTTCCT GGACCATCCT GGAGGAGAGC CAGAAGTGTC ACCAGAGGTT CAAGTCCCTG 
CAGAGCAAGC TAGCCACCCA ACACCAGCAA CTGCAGGCCT CAGTTGAGCT GTATGAATTT 
AATCTCCTTA GCAACCTGGA GCTCACATGG GCAGCTGAGC ACATGCCCAA TGCCGCTCTC 
AACTGCCCTG CCCAGTGCTG GCATGATGCC CACAGCCTTC AACGAAAGCA CAAGATGCTC 
CAGGCTGAGG TGAAAGGTCA TGTAAGGCAC ATGTACAGGG TGCTAAGTTC TGGTCAGCGT 
CTAGCAGCCT CTGGGCACCC TCGAGCTCAG CATATCGTGG AACAGTGCCA GAAGCTAGAA 
AGCCACTGGG CAGGACTGGA GCAGGCGTGT GAAGAACGTG CACACTGCCT GCAACAGGCT 
GTCACTGTCC AGCAGTACTT TCTGAACGTG TCAGAGATGG AGACCTGGGT GGAGGAAAAG 
CGGCCTCTGG CAAGCAGTCA GGACTACGGC AGTAATGAGG AAGCTACCAG TGGGCTCATT 
AGGAAACACC AGATGCTCCA GCAGGAGGTA GCTCTTTATT GGAGCTCCAT GGAAGATCTC 
GAGCAGAGGT TCCAGACCCT TGCTGAATTT GAGGCCCCTG AGCGGTTGGG TGTGGTACGG 
GAGAAGTTGC AGGCACTACG GAAGCTGGCA GACGAACGGG GGCAGGAGCT GGAGGGGACC 
CTGAGACTAC ATGAGTTCAT GAGAGAAGCT GAGGACCTGC AGAGCTGGCT GTCCAGCAGG 
AAGCAGGTGG CCAGAGGAGG GGACACCTTT GGAGAGGACC ATGAGCACGT GCTGCAACTC 
TGCACTGAGT TTACAAAGTT TCAGTACCAG GTTGAAACTG GTGCCCAGCG AGTGGAAACC 
TGTCGACTGC TGGCAGAGAG CCTACAAGAG CGTGGGCACA GTGCTGCCCC CAAGGCTCAC 
CAGAGGCAGC AGGATATACA GGCTTCCTGG TCTGAACTGT GCCAGTTGAC CCAGGCCCGA 
AGCCGTCTGC TCAATGACGC CGAAATCACT CTTAGAGTCC ACGGAGACCT TCTGGAAGTC 
CTTACCCAGA TTCAGGAGAA AGCCACAAGC CTCCCTAACG ATGTGGCCCA GGACCTGTGT 
GGGGTAGAGA ACCAGCTCCA GAGACATGAG AGGCTGGAAC GCGAGTTGGC AGGCATGGAG 
CAGCAGGTGC AGGAACTGAT GAAGGCTGGA GGCAGAGTGC AGGAGCTGTG TCCAGGAACT 
CAGGCCCTTG CCGCTGTCCA GCAAAAGCAG CAAGCTGTGA CCCAAGCTTG GGAGGCCCTC 
CAGCTGCGCA TGGAGCAGCG CAAGGCTCAA TTGGAACGAG GATATCTCCT GGTCCGGTTC 
CACACAGCGG TGAGGGACTA TACCTCCTGG GCGGCCAGCG TGCATCAGGA GCTACAGATG 
GAGGAGGCCT CCTGGGAACC CCACAGCCTT TTGCTCAGGC TCAGAGCCCA TCAATGGCTG 
TGGGCAGAAC TGAAAGCCAA GGAGGAGCTG CAGCAACGGG CCACTAAGAT GGGCCAGCAA 
GCTCTGCTGG CAGCAGGAAC ACCCGCTAAG GTCCAGGATG GGCTTCGAAC CCTACAGGAG 
GAACGCGACC AAGTGTTCCA GGCCTGGGCA CTCAAGCAAG AGAAGCTGCA GGCCATGCTT 
CAAGAACAGC ACCTCCTCAG GCAATGCGGC CACCTAGTGG AGGTCCTCAC AGCCCAGGAG 
GCATTCCTAA AAGCCAGTGG CCTGGGGAGC TCAGTGGAAG AAGTGGAGCA GTTGATCCGA 
AAGCACGTGA TCTTCCAGAA AGTGCTAGCT CTCCAGGACA AGAAGGAGTC GGCTCTGAAT 
GAGCAGCTAA AGACGATTTC AAGCCCTAAG GGGCAGAACC TGTTTTGCCA CATGTTGGAG 
CACCGGGCTC AAGTGAAGGA ACTGGCAGAG AGCCGGGGCC AGGCTCTGCA CACCTCCCTG 
ATGATAGGCA ACTTTGTCAG GGCAGCAACC CAGGCTGAGG ACTGGATCCA GCAGCGGGTC 
CAGCAACTGA GAGCCTGGAG CCCTCTTGGG AATCTAAAGG ATTATCTGAA GCACCTGAGG 
AAACACCAGG CCTTCAGGGC TGAGGTCCAG GCTCAGGAGC AGATCTTGAC CTCTGTTGCC 
AAGCAAGGCG AAGAGCTCCT CAGTCAGAGC CACCCTCAAG CTGGAGAGGT CTCCCAGAGG 
CTGGAGGCCC TGCGGGACCT CTGGGAGAAG CTGAGGCAGG CAGTGACCCT GCAGGGCCAG 
GCCCTAGAGA ACAGATACAA CTTCCAGGAG TTCCTGCAGA GAGTGGACCT AGCAGAGACC 
TGGATCCAGG AGAAGGAGAG GATGGTGAAC AGCTGTGACA TTGGCCTGAA CCTTGAGCAC 
TGCCTGCAGC TCTGCAGACA AGTCCGCAGG TTACAGGTCA CAGTGGATGA TGCCCACATC 
AAGGGAATCA AAAACTTGTC ACTGCAGCTC AAGAACCAGG GCCCTGAGGA ATCCGAGACC 
ATCTGCCAGC GGCAAAACCA GCTCAACAAT AGGTGGAAGA CTTTCCATGG CAACCTGCTC 
CTCTATCAGC AGCGGCTTGA AGCAGCCCTG GAGATACATA GATTGTCCAG GGAACTGGAT 
GACGTTATTG AGCGGCTCAG GGAGAAGGAA TCCCTGATCT GGGCCCCAGA GGGCACTGAA 
GATTTGGAGA ATGTGCAGAG GCTATCATGG AGACAGAAAG TGCTGCAGCA GGAGATGGGC 
CTCATCCAGA CCCAGGTGGA GTCTCTTGAA GGTAGGATTG GCCGGCTCTG CAAGGAAAGT 
CCCGAGGTTG CCCACAGCCT CAGACATAAG CAGCAAGAAA TGATGGACAG CTGGTGGAAG 
GTCTGGAGCA AAGCCCAGAA GTGGAGGGAG TTGCTGGATG TGGGCCATGA AGTTCAGAAA 
CTCCAGACCC TGCTGCAGGA TCTGCAGGAC TGGGCCCGGG GACTACAGGC TGAGATGGCC 
ACACAGGGCA CCCCCTGCAG CCCTGTGAGA ATCCAGTACA TGTTGGAAGA GTACCGGGCA 
TACAAGGTTG AGCTGGACAT CCGAACAGAG AGCCTTAACC TGGTCCAAAG CATGGGGCAG 
CGGCTGCTTG CATCTGGCTA CCCACAGGCC TCTGAGATTC ACCAGACCCT GGCCGCTGTA 
GAGCAGGGGT TGAGTAGCTT GCGGGAATCC TGGCAGGGCC GGCAACAGCA GCTACAGCAG 
GCCTTGGAGC AGCAGCTGTT TCTGGGCTCT GTGGAAAAGG CGGAACGCTG GCTTGACAGT 
GAAGAGGCCT CCCTGGCTAG TGAGGGAGTG GCGGACCCCT TGGTCACTGT GGAGACCCTT 
CTGTCAAAGC TCAAGAGGCA TGAGCAGGGC CTGAAGGCCC AGGCCGAAAA GATCCGTGCA 
TTGGAGGCCA CAGCCCACAG CCTGCACCAG GGTGGACACT CTGAGGCGCG TAGCATCCTG 
GACAGATGCC AGGCCCTGCT CCTGAGGACA GAGGCACTCA CAGAGCAAGC GAGGGCCCGG 
GGACACCAGC TGGAGGAGCT GCGGAAGCTT CGGACATTTT TGCAGGATTC CAATGAGGTA 
ACTGCATGGC TGAGGGAGAA GAGCCTGGCG GCTCTGGATG AGGGCCAGCA GGACCCAGCC 
ACGATGCAAA CACAGTTGCA GAAGCAGCAG AATTTCCAGG CTGAGCTGGA CGCCAGTGTT 
CACCAACAGC AGGAGGTCCA GATGGAGGGG CAGAAGCTGC TACAAGGGGG CCACCCGGCC 
TCAGAGACCA TCCGGGGGCA ACTGGAAGAA TTAGGAGGCC TCTGGGATGA GCTGCAGACC 
AACTGCCAGA GGAAGATGGC CAGACTACAG GGGGCCCTCA AGGTCCTGCA TCTGCAGAGA 
ATGCTGAAGG AACTGGAGAA GTGGCTGGAG CACATGGAAG CAGAGCTGAG AGTCCCTGTC 
AGAAGCCAGG CGCTGCCTAG AGTGGGCGAA CTCCTGGGAG CCCAGGAGGA GCTGGAAGCA 
GCCATGGACA GGCAGGCCAA GGAAGTGCAA GAGTTGCAGG GCCAGTCCCA AGCCTGCCTC 
CAGGAAGGCC ACTGCCTCGC CAAAGATGTG GAAGAGCAAG CCCGGCAGCT GCTTCAGAGG 
TTTCAGAGCC TTCGGGAGCC CCTACAAGAG CGCAGGGCAT CCCTGGAAGC CCAAAGGCTC 
CTCTTACAGT TCTTCAGGGA TGCTGATGAG GAAATGGCCT GGGTGCAGGA GAAGCTGCCC 
TCAGCCACAG CCCAGGACTA CGGCCAGAGC CTTAACACTG TGCGCCACCT GCAGGAGAAA 
CACCAGAATT TGGAGAATGA GATCCATAGC CACAAGGCTC TGAGCCAGGT GGTGACAGGT 
ACAGGGCACA AGCTCATACA GGCAGGGCAC TTTGCCACTG AAGAGGTGGC CGCCCGGGTG 
CAGCAGCTGG AGGTGGCTCT AAACCGCTTA GAGACAGAGG CCGCACAGAG GAGGAGGAGA 
CTGCAGCAGG CCCTGGAGGC CCAGCAGACT TTGGTGGAGC TCCTGGAAGC AGGATCCTGG 
CTGGCTGAAC GGGACCATAT TCTGGACAGT GAGGACCTGG GCCAGGACGC TGAGGCCACA 
CAGGCTCTTC TGCGGTGCCT AGAGGCCACC ACAAGAGACT TGGAAGGGTT CAGCAGTCGC 
ATTGAGCAGT TACAGCAAAC AGTTGCCCTT CTGGAGAGTG GACAGACCCC AGGCAGTCCT 
AGGGTGCTGG CCCAGCTGCA GGCTGTGAGA GAGGCCCATG CACGGCTGCT GCAGAGAGCA 
GAAAGCAGGG GGGAGGCGCT GCGTGAACAG CTGCACCTGT ACCAGCTGGA GCAAGAGGCC 
TTGCTCCTGG ATGCCTGGCT GACCACCAAG CTGGCCGTCG CTGAGTCTCA GGACTATGGA 
CAGGATCTAG CAGGCATCAA GGTGCTGGAA GACATGTTTG GTGCTTTCAA CAGAGAAGTC 
CAGAGCCTGG GCCAGGCCAA GATGCAGACC CTGAGGGAGC GGATGGCTTC CCTAGAAAGA 
GGAGCACCCA GGTTCTACCC CCAGATTCAA GCTCAGAAGT GTCGCGTCCA GGCCGCTTGG 
GAGGGGCTGA ACAAGGCAAT CAAAGTCCGT ACAGAGAACC TGGCAGCAGC CTGCGATCTC 
CGGAGCTTTG AGCAGGCTGC CTCGGAGCTG CAGAGATGGA TACAGGAGAA GACCACCCTG 
CTGGAGGAAG CATTCCAAGT CCACAGCCTG TCACCTTCAC AGCCCCTTCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC ATAGGCGCCT GCAGAGAGAA CTGAGGGCTA TTGAAAAAGA GGTGTCAAGA 
GTGCAGATGG AGGCCCATCG GCTCGGCCAG CACTACCCTG TGGCTCAGGG GAGTCTTAGT 
GAGTGGCTCA CCAAGGTGCA GGGGGCCTGG ACCAACCTGG AGGCCAAAGT CCAGGAATGG 
AGCCAGAAGC TGTTGCAGGC TACCCAGGGC CACACCTTTC TTGGGAGCTG CCGGGAATTG 
CTAGCGTGGG CTCAGGAGAT GCAGGAGCTG CTTTCTAAGG AGAAGCAGGC TGGGGATGTG 
GTGGGGGCCA AGCAGTTCCT TGAGCAGCAT GAGGCGCTGG AGCAAGAGAT TCAGGAACGC 
TGCCTTCAAG CCCAGACCAT ACGGCACGAA GGGCAGCAGC TGCTGGACAA CGGCCACTTC 
CTGTCCCCAG AGGTGGCAGA GTGTATGCAG GAGCTGGAAA GACATCTGCA GGAGCTTCAG 
GTAGCCTGGG CCCTGCGTGG ACAACGCTGG GAACAGACCC GGAGCCTGCA ACAGCTGCGG 
CAGAGGCTGG AGCTGGCTGA GGCTTGGCTG GCCTCCTGGG AGCGCCTGCT GCTGGACCCC 
AGCTGTGGGC ACTCGGTGTT GGAAGTGGAA CGCCTGCTCT ACAGACACGA GGGCTTAGAA 
AAGCTGCTGG TGGCCCACGA GGAGACATTC ATTCAGCTTC AGACGATGAC AGAGGAGGCA 
AAGGGTGGTC ATGTCACGGA GGCATCTGTG GAACAGCAGC CCCCCACAGA AGGGAGGCAG 
CCCAGAACCA GCTTCCCAAC CAGCAGCCAT GGGATCTTGG CAAGGGGAGC CTCGAGCCCA 
TTCTCAGATA TGAGGAAGGC AGAAGTGCCA AGTGGCACTG GGGAACTGGC TTACGTGTCC 
CCCCTTGTAC CTGAGGAGAC TGAATGTGAG AGGCTGGAGG ACAGAAGGCA GACTTTCTTC 
CCAAGAATCC CAAAGGAACC CTGGATTTTT CCACCTCCCT GCAGTGAGCT CTCAAGGAGT 
CTGCTTGGAA GTGTTGAGCG CTCACCCACA CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107791.1
CDS	1922..13117
Translation	MGTVYQDRASDSWQKDHQFPQYFPAPMPPPPPDTGKCPVAIHPQRAKAHKLVVPSTLHLLSRSVPDEKTQAQSSALLSSSLAMDSEYEIGHVRKLQAQHTHMQEKTFTNWINNIFRLGRVGIRIQNLYTEFADGAHLLRLLELISGEALPAPSPGRLRVHFLENNSHALAFLRAKVPIPFIGPENIVDGDQSLILGLLWVIILRFQISHISLDREEFGASAALLSAKEALLVWCQRKTAGYTNVDITDFSRSWSDGLGFNALLHAHRPDLLDYGSLSPDRPLYNLSFAFRVAEQQLGIAQLLDPEDVAALHPDECSIMTYLSQYYHYFSRLQRGHTAQRRLAKILLQLQETEVLQAQYKQLVADLLCWIEEKKMQLEARDFPDSLPAMRQLLAAFASFRAREKPPRWQQRGATEALLFQLQTTLRAQNRRPFLPREGLGPAELAQRWAELERAEACRSQAMQQRLLQLERLDTLARRFQCKASHRESFLNDVEQMLDQARASLTDPATVEAATQRLSVLEAAILPQEGRFQALGEMADILRQEEYHSWADMARRQKEITGRWRRLLRCLQEERKRMEDSKAVLSLLQGVEAVTHQLGELQVLASSTVYGQQLAEIVSLLQSHDLLEAQVSAHRTHVTHLVHQTTQLDSQGTSVEVLQAKALALAELHHSLVSLVRARRTLLEQTLQRAQFLRSCEEEEAWLQEHRQLMETAVLDRDLTQIATALQKHKALETELHRHQAVCVDLMQRGRNFSVREPLTQPDPLERAEAVQGTWQLLWAGAARRRARLQTALLIGQYFSDSAEAASWLFQRQKQLESASCGKDQADAEALLLQHLRLEQDVRAFAAELRELEEQARAAAALVSHMVHTGTWKGSRLVSPTPNEEPPEDWKRLGRDTIGGPQGETQVLAAPGSHYSHQQIAFFSREEAPNTWKLEELGDSQSRMAIIHSIEKKPQVLSALGFLGEGPRTVLQAENDVHFNSSTILQAQADVSQTYEKLRALAKLHRTRLEESIALFSFYSSCRELQSWLEKQTALFQTLQPQGHNLEVIQLKYENVLMTLATGKGHWAEAINTAEQLKQRCPGHISKIQQQQEDLKQRWQQLEALKEEKLLQLTRGMEVCSLLQESEPIQAQLLNVISRLETLGTRSSGDSHHTLQQTQQKVLVLEKKISYLQRATIEVMESGSPESRLLWEQVLMLQSLLKQAQGQVARQIQVQTVARVQRGILQESQKLLLWAEDIQAQLCSKEERQVEASALRPLRRHGTLQEETCLWEERLQQLEAQGQPVAVSDSPQSQEVASALRLLGQHSQQLKALWEQRQQKFWEGLELQGFGQEVDDFMATCASHEALLQLDNLGEDIREAQSLLQQHQGLGWLRSTLGSRAEALRARGEKLLLSHPAAVSKIRELLHSAQAQWTRVQERSEQRRVQLLASLQLQEWKQAEEGLMLWMEEKWPRVADERSQAGSNILQKLKWRKITKSELLASRRYMEELQQAGKELLHSNPYAQEDIQDRLQSLNHKWEELNHKMEDRGDRLPQTRQQDQLLELIQDVQETMEHLEGALQSTETGQDLCSSRRLQRQHCKLEDKSQALASKMDALISQTHNAFTSWTILEESQKCHQRFKSLQSKLATQHQQLQASVELYEFNLLSNLELTWAAEHMPNAALNCPAQCWHDAHSLQRKHKMLQAEVKGHVRHMYRVLSSGQRLAASGHPRAQHIVEQCQKLESHWAGLEQACEERAHCLQQAVTVQQYFLNVSEMETWVEEKRPLASSQDYGSNEEATSGLIRKHQMLQQEVALYWSSMEDLEQRFQTLAEFEAPERLGVVREKLQALRKLADERGQELEGTLRLHEFMREAEDLQSWLSSRKQVARGGDTFGEDHEHVLQLCTEFTKFQYQVETGAQRVETCRLLAESLQERGHSAAPKAHQRQQDIQASWSELCQLTQARSRLLNDAEITLRVHGDLLEVLTQIQEKATSLPNDVAQDLCGVENQLQRHERLERELAGMEQQVQELMKAGGRVQELCPGTQALAAVQQKQQAVTQAWEALQLRMEQRKAQLERGYLLVRFHTAVRDYTSWAASVHQELQMEEASWEPHSLLLRLRAHQWLWAELKAKEELQQRATKMGQQALLAAGTPAKVQDGLRTLQEERDQVFQAWALKQEKLQAMLQEQHLLRQCGHLVEVLTAQEAFLKASGLGSSVEEVEQLIRKHVIFQKVLALQDKKESALNEQLKTISSPKGQNLFCHMLEHRAQVKELAESRGQALHTSLMIGNFVRAATQAEDWIQQRVQQLRAWSPLGNLKDYLKHLRKHQAFRAEVQAQEQILTSVAKQGEELLSQSHPQAGEVSQRLEALRDLWEKLRQAVTLQGQALENRYNFQEFLQRVDLAETWIQEKERMVNSCDIGLNLEHCLQLCRQVRRLQVTVDDAHIKGIKNLSLQLKNQGPEESETICQRQNQLNNRWKTFHGNLLLYQQRLEAALEIHRLSRELDDVIERLREKESLIWAPEGTEDLENVQRLSWRQKVLQQEMGLIQTQVESLEGRIGRLCKESPEVAHSLRHKQQEMMDSWWKVWSKAQKWRELLDVGHEVQKLQTLLQDLQDWARGLQAEMATQGTPCSPVRIQYMLEEYRAYKVELDIRTESLNLVQSMGQRLLASGYPQASEIHQTLAAVEQGLSSLRESWQGRQQQLQQALEQQLFLGSVEKAERWLDSEEASLASEGVADPLVTVETLLSKLKRHEQGLKAQAEKIRALEATAHSLHQGGHSEARSILDRCQALLLRTEALTEQARARGHQLEELRKLRTFLQDSNEVTAWLREKSLAALDEGQQDPATMQTQLQKQQNFQAELDASVHQQQEVQMEGQKLLQGGHPASETIRGQLEELGGLWDELQTNCQRKMARLQGALKVLHLQRMLKELEKWLEHMEAELRVPVRSQALPRVGELLGAQEELEAAMDRQAKEVQELQGQSQACLQEGHCLAKDVEEQARQLLQRFQSLREPLQERRASLEAQRLLLQFFRDADEEMAWVQEKLPSATAQDYGQSLNTVRHLQEKHQNLENEIHSHKALSQVVTGTGHKLIQAGHFATEEVAARVQQLEVALNRLETEAAQRRRRLQQALEAQQTLVELLEAGSWLAERDHILDSEDLGQDAEATQALLRCLEATTRDLEGFSSRIEQLQQTVALLESGQTPGSPRVLAQLQAVREAHARLLQRAESRGEALREQLHLYQLEQEALLLDAWLTTKLAVAESQDYGQDLAGIKVLEDMFGAFNREVQSLGQAKMQTLRERMASLERGAPRFYPQIQAQKCRVQAAWEGLNKAIKVRTENLAAACDLRSFEQAASELQRWIQEKTTLLEEAFQVHSLSPSQPLLQQQQQQQQQQQQQQQQQQQQQQQQQQHRRLQRELRAIEKEVSRVQMEAHRLGQHYPVAQGSLSEWLTKVQGAWTNLEAKVQEWSQKLLQATQGHTFLGSCRELLAWAQEMQELLSKEKQAGDVVGAKQFLEQHEALEQEIQERCLQAQTIRHEGQQLLDNGHFLSPEVAECMQELERHLQELQVAWALRGQRWEQTRSLQQLRQRLELAEAWLASWERLLLDPSCGHSVLEVERLLYRHEGLEKLLVAHEETFIQLQTMTEEAKGGHVTEASVEQQPPTEGRQPRTSFPTSSHGILARGASSPFSDMRKAEVPSGTGELAYVSPLVPEETECERLEDRRQTFFPRIPKEPWIFPPPCSELSRSLLGSVERSPTL

Target ORF information:

RefSeq Version	XM_030251931.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 5 (Sptbn5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251931.1

ORF Insert Sequence:

ATGGGGACAG TGTATCAAGA TAGGGCCTCA GACTCCTGGC AGAAGGATCA TCAGTTTCCA 
CAGTACTTCC CAGCCCCCAT GCCTCCACCA CCTCCAGACA CAGGGAAATG CCCAGTCGCC 
ATTCACCCTC AAAGAGCTAA GGCTCATAAG CTGGTTGTCC CATCAACCCT CCACCTCCTT 
AGCCGCAGCG TCCCTGACGA GAAGACCCAG GCCCAAAGCT CAGCCCTTCT GAGTTCAAGT 
TTGGCCATGG ACTCTGAGTA TGAGATAGGC CATGTCCGCA AGCTCCAGGC TCAGCATACA 
CACATGCAGG AGAAGACCTT CACCAACTGG ATCAATAACA TCTTCCGACT AGGACGGGTG 
GGCATCAGGA TCCAGAACCT GTACACAGAG TTTGCTGACG GTGCCCACCT ACTGCGGCTA 
TTGGAGCTTA TCTCTGGGGA GGCCCTACCA GCGCCCAGTC CGGGCCGCCT GCGCGTGCAC 
TTCCTGGAGA ACAACAGCCA CGCCCTGGCC TTCCTCAGGG CCAAGGTGCC CATTCCCTTC 
ATCGGGCCAG AGAACATTGT GGATGGGGAC CAGTCACTCA TCCTGGGACT CCTCTGGGTC 
ATCATTCTGC GTTTCCAGAT TTCCCACATC TCCTTGGACA GGGAGGAGTT TGGGGCCAGT 
GCGGCTTTGC TGTCTGCCAA GGAAGCCCTG CTGGTCTGGT GTCAGCGGAA GACAGCCGGC 
TACACCAATG TGGACATCAC TGACTTCTCC CGAAGCTGGA GTGACGGGCT CGGCTTTAAT 
GCCCTGCTCC ACGCTCACAG GCCGGACCTG CTGGATTATG GCTCGCTGAG TCCAGACCGC 
CCTCTGTATA ACCTCTCCTT TGCCTTCCGA GTGGCTGAGC AGCAGCTTGG CATTGCCCAG 
CTGCTCGACC CTGAGGATGT GGCAGCCCTG CATCCAGATG AGTGCTCCAT CATGACCTAC 
CTGTCTCAGT ACTACCACTA CTTCTCCCGT CTGCAGCGGG GGCACACGGC CCAGAGGAGA 
CTGGCTAAGA TCCTGCTGCA GCTGCAGGAG ACGGAGGTAT TGCAGGCACA ATACAAGCAA 
CTGGTGGCTG ACCTGCTCTG CTGGATTGAG GAGAAGAAGA TGCAGCTAGA AGCTCGGGAC 
TTCCCAGACT CACTGCCTGC CATGCGCCAG CTACTGGCAG CCTTTGCCTC CTTCCGTGCC 
CGGGAAAAGC CACCTCGGTG GCAGCAGCGA GGAGCCACAG AGGCCCTGCT CTTCCAGCTG 
CAGACAACAC TCCGAGCCCA AAACCGAAGG CCATTCCTAC CTCGAGAGGG CCTGGGCCCT 
GCAGAGCTGG CCCAGCGCTG GGCAGAACTG GAGAGGGCAG AGGCCTGCAG GAGCCAGGCC 
ATGCAGCAAA GGCTGCTACA GCTGGAGCGA CTGGACACTC TGGCCCGCCG CTTTCAGTGC 
AAGGCATCCC ACCGGGAAAG CTTTTTAAAT GACGTAGAGC AGATGTTAGA CCAGGCCAGA 
GCCTCCCTCA CCGACCCAGC CACAGTGGAG GCAGCCACTC AGAGGCTGAG CGTTCTGGAG 
GCTGCCATCC TGCCCCAGGA AGGGCGCTTC CAGGCCTTGG GTGAGATGGC AGACATCCTC 
CGGCAGGAAG AGTATCACAG CTGGGCGGAT ATGGCCCGCA GGCAGAAGGA GATCACGGGG 
CGCTGGCGGA GGCTCCTACG GTGTCTACAG GAGGAGAGGA AGCGCATGGA AGACTCAAAG 
GCTGTGCTGA GTCTGCTGCA GGGGGTGGAG GCTGTCACTC ACCAGCTTGG GGAGCTGCAG 
GTGTTGGCCA GCTCCACAGT CTATGGGCAA CAGCTGGCAG AAATAGTGTC GCTGCTGCAG 
AGCCATGACC TGCTGGAGGC CCAGGTGTCA GCCCACAGGA CCCATGTGAC TCATCTTGTC 
CACCAGACCA CCCAGCTAGA CTCCCAGGGT ACCAGTGTGG AGGTGCTGCA GGCTAAAGCC 
CTAGCACTGG CCGAGCTCCA CCACAGCCTG GTGTCTCTTG TCAGAGCCCG ACGTACCCTG 
CTGGAGCAGA CCCTCCAGCG AGCACAGTTT CTGCGCAGCT GTGAGGAGGA GGAGGCCTGG 
TTGCAAGAGC ACAGACAGCT AATGGAGACC GCGGTCCTGG ACCGGGACCT CACTCAGATC 
GCTACTGCTC TGCAGAAACA CAAGGCTCTG GAAACAGAAC TCCATCGCCA CCAGGCAGTG 
TGTGTAGATC TCATGCAGAG AGGACGCAAC TTCAGTGTCA GAGAGCCCCT GACACAGCCA 
GATCCACTGG AAAGGGCAGA AGCAGTGCAG GGAACCTGGC AGCTGCTCTG GGCTGGAGCC 
GCAAGGCGGC GCGCCCGGCT GCAAACAGCA CTGCTAATTG GGCAGTACTT TTCTGACTCG 
GCCGAAGCAG CTTCCTGGCT TTTCCAGCGG CAGAAACAAC TAGAGAGCGC ATCCTGCGGG 
AAGGACCAGG CAGACGCCGA GGCCCTGTTG CTGCAACACC TGCGCCTGGA GCAAGACGTG 
CGCGCCTTCG CGGCGGAGCT GCGCGAGCTA GAGGAGCAGG CGCGGGCTGC CGCAGCCCTA 
GTGTCACACA TGGTGCACAC GGGGACATGG AAAGGATCCA GACTTGTCTC TCCTACACCA 
AATGAGGAAC CTCCAGAAGA CTGGAAAAGG CTTGGCAGAG ACACCATTGG AGGACCCCAG 
GGGGAGACTC AAGTTTTAGC AGCACCAGGC AGCCATTATT CACATCAGCA GATAGCCTTC 
TTCTCCAGAG AAGAGGCTCC AAATACCTGG AAGCTTGAAG AGTTGGGAGA CAGTCAGAGC 
AGGATGGCCA TCATCCACAG CATCGAGAAG AAACCCCAGG TGCTATCTGC CCTGGGCTTT 
CTAGGAGAAG GCCCGAGGAC AGTTCTGCAG GCTGAGAATG ACGTTCACTT CAACTCCAGC 
ACCATACTCC AGGCTCAGGC TGACGTGAGC CAGACCTATG AGAAGCTTAG GGCCCTGGCA 
AAGCTTCACA GGACCCGACT GGAGGAGTCA ATCGCCCTGT TTAGCTTTTA TAGCTCCTGT 
AGGGAGCTTC AGTCCTGGCT GGAGAAGCAG ACCGCACTGT TCCAAACATT GCAGCCCCAG 
GGACACAACT TAGAGGTCAT ACAACTCAAA TATGAGAATG TTCTCATGAC CTTGGCCACT 
GGAAAAGGCC ACTGGGCAGA GGCCATCAAC ACTGCTGAAC AACTGAAGCA GAGATGTCCA 
GGACACATCT CGAAAATCCA ACAACAACAG GAGGACCTGA AGCAAAGGTG GCAGCAGCTG 
GAAGCCCTGA AGGAGGAGAA ACTCCTGCAG CTGACACGTG GCATGGAGGT GTGCAGTCTT 
TTACAGGAGT CTGAACCCAT ACAAGCCCAA CTGCTAAATG TGATAAGCAG GCTGGAGACT 
CTGGGAACAA GAAGCTCTGG GGACAGCCAT CATACCCTAC AGCAGACCCA GCAGAAGGTC 
CTGGTATTGG AGAAGAAGAT CTCCTACCTC CAAAGAGCAA CCATAGAGGT GATGGAGTCA 
GGCTCCCCAG AGAGCAGGCT GCTTTGGGAG CAGGTCCTGA TGCTGCAGTC ACTGCTAAAG 
CAAGCGCAAG GGCAGGTGGC CCGGCAGATC CAGGTCCAGA CTGTGGCTCG GGTTCAGCGA 
GGCATCCTCC AGGAGAGCCA GAAGCTGCTG CTGTGGGCAG AGGACATCCA GGCTCAGCTG 
TGCAGCAAAG AGGAGCGGCA GGTTGAGGCT TCAGCCCTGC GGCCGCTGAG GAGGCATGGA 
ACCCTACAGG AGGAGACCTG CCTGTGGGAA GAGAGGCTGC AGCAGCTGGA GGCTCAGGGT 
CAGCCGGTGG CAGTCTCAGA CTCTCCACAG TCCCAGGAGG TGGCCAGCGC TCTAAGGCTC 
CTAGGCCAGC ACAGCCAGCA GCTGAAGGCT CTGTGGGAGC AGAGACAGCA GAAGTTTTGG 
GAGGGGCTGG AGCTGCAGGG GTTTGGCCAA GAAGTAGATG ATTTCATGGC CACCTGTGCC 
AGCCATGAGG CCTTACTGCA GCTGGACAAC CTTGGGGAGG ACATAAGGGA GGCCCAGAGC 
CTGCTGCAGC AGCACCAGGG TTTGGGGTGG CTCCGCAGCA CCCTGGGATC ACGGGCAGAG 
GCCCTCCGTG CACGCGGTGA GAAGCTGCTT CTGAGCCATC CAGCTGCCGT GAGCAAGATC 
AGAGAGCTGC TGCACAGTGC CCAGGCCCAG TGGACCAGGG TCCAAGAGAG GAGTGAGCAG 
AGAAGGGTCC AGCTGCTGGC TTCCCTCCAA TTGCAGGAAT GGAAGCAGGC TGAAGAAGGC 
CTAATGCTGT GGATGGAGGA GAAGTGGCCC AGGGTGGCTG ATGAGCGCTC CCAAGCAGGA 
AGCAACATTC TGCAGAAACT CAAGTGGCGC AAAATAACTA AGAGTGAGCT ATTAGCCTCT 
CGGAGGTACA TGGAAGAGCT ACAGCAGGCT GGGAAAGAAC TGTTGCATAG CAACCCATAT 
GCACAGGAGG ACATACAGGA CAGACTTCAA AGCCTTAATC ACAAATGGGA AGAGTTGAAC 
CACAAGATGG AAGATCGAGG GGATAGGCTG CCTCAGACCA GACAGCAGGA CCAGCTCCTA 
GAACTAATAC AGGATGTCCA GGAGACAATG GAGCATCTGG AGGGAGCCCT GCAGAGCACA 
GAAACCGGGC AGGACCTGTG CTCCAGCCGG AGGCTGCAGA GACAGCATTG CAAGCTAGAG 
GACAAGAGCC AGGCACTGGC CAGCAAGATG GATGCCCTCA TCTCTCAGAC CCACAATGCA 
TTCACTTCCT GGACCATCCT GGAGGAGAGC CAGAAGTGTC ACCAGAGGTT CAAGTCCCTG 
CAGAGCAAGC TAGCCACCCA ACACCAGCAA CTGCAGGCCT CAGTTGAGCT GTATGAATTT 
AATCTCCTTA GCAACCTGGA GCTCACATGG GCAGCTGAGC ACATGCCCAA TGCCGCTCTC 
AACTGCCCTG CCCAGTGCTG GCATGATGCC CACAGCCTTC AACGAAAGCA CAAGATGCTC 
CAGGCTGAGG TGAAAGGTCA TGTAAGGCAC ATGTACAGGG TGCTAAGTTC TGGTCAGCGT 
CTAGCAGCCT CTGGGCACCC TCGAGCTCAG CATATCGTGG AACAGTGCCA GAAGCTAGAA 
AGCCACTGGG CAGGACTGGA GCAGGCGTGT GAAGAACGTG CACACTGCCT GCAACAGGCT 
GTCACTGTCC AGCAGTACTT TCTGAACGTG TCAGAGATGG AGACCTGGGT GGAGGAAAAG 
CGGCCTCTGG CAAGCAGTCA GGACTACGGC AGTAATGAGG AAGCTACCAG TGGGCTCATT 
AGGAAACACC AGATGCTCCA GCAGGAGGTA GCTCTTTATT GGAGCTCCAT GGAAGATCTC 
GAGCAGAGGT TCCAGACCCT TGCTGAATTT GAGGCCCCTG AGCGGTTGGG TGTGGTACGG 
GAGAAGTTGC AGGCACTACG GAAGCTGGCA GACGAACGGG GGCAGGAGCT GGAGGGGACC 
CTGAGACTAC ATGAGTTCAT GAGAGAAGCT GAGGACCTGC AGAGCTGGCT GTCCAGCAGG 
AAGCAGGTGG CCAGAGGAGG GGACACCTTT GGAGAGGACC ATGAGCACGT GCTGCAACTC 
TGCACTGAGT TTACAAAGTT TCAGTACCAG GTTGAAACTG GTGCCCAGCG AGTGGAAACC 
TGTCGACTGC TGGCAGAGAG CCTACAAGAG CGTGGGCACA GTGCTGCCCC CAAGGCTCAC 
CAGAGGCAGC AGGATATACA GGCTTCCTGG TCTGAACTGT GCCAGTTGAC CCAGGCCCGA 
AGCCGTCTGC TCAATGACGC CGAAATCACT CTTAGAGTCC ACGGAGACCT TCTGGAAGTC 
CTTACCCAGA TTCAGGAGAA AGCCACAAGC CTCCCTAACG ATGTGGCCCA GGACCTGTGT 
GGGGTAGAGA ACCAGCTCCA GAGACATGAG AGGCTGGAAC GCGAGTTGGC AGGCATGGAG 
CAGCAGGTGC AGGAACTGAT GAAGGCTGGA GGCAGAGTGC AGGAGCTGTG TCCAGGAACT 
CAGGCCCTTG CCGCTGTCCA GCAAAAGCAG CAAGCTGTGA CCCAAGCTTG GGAGGCCCTC 
CAGCTGCGCA TGGAGCAGCG CAAGGCTCAA TTGGAACGAG GATATCTCCT GGTCCGGTTC 
CACACAGCGG TGAGGGACTA TACCTCCTGG GCGGCCAGCG TGCATCAGGA GCTACAGATG 
GAGGAGGCCT CCTGGGAACC CCACAGCCTT TTGCTCAGGC TCAGAGCCCA TCAATGGCTG 
TGGGCAGAAC TGAAAGCCAA GGAGGAGCTG CAGCAACGGG CCACTAAGAT GGGCCAGCAA 
GCTCTGCTGG CAGCAGGAAC ACCCGCTAAG GTCCAGGATG GGCTTCGAAC CCTACAGGAG 
GAACGCGACC AAGTGTTCCA GGCCTGGGCA CTCAAGCAAG AGAAGCTGCA GGCCATGCTT 
CAAGAACAGC ACCTCCTCAG GCAATGCGGC CACCTAGTGG AGGTCCTCAC AGCCCAGGAG 
GCATTCCTAA AAGCCAGTGG CCTGGGGAGC TCAGTGGAAG AAGTGGAGCA GTTGATCCGA 
AAGCACGTGA TCTTCCAGAA AGTGCTAGCT CTCCAGGACA AGAAGGAGTC GGCTCTGAAT 
GAGCAGCTAA AGACGATTTC AAGCCCTAAG GGGCAGAACC TGTTTTGCCA CATGTTGGAG 
CACCGGGCTC AAGTGAAGGA ACTGGCAGAG AGCCGGGGCC AGGCTCTGCA CACCTCCCTG 
ATGATAGGCA ACTTTGTCAG GGCAGCAACC CAGGCTGAGG ACTGGATCCA GCAGCGGGTC 
CAGCAACTGA GAGCCTGGAG CCCTCTTGGG AATCTAAAGG ATTATCTGAA GCACCTGAGG 
AAACACCAGG CCTTCAGGGC TGAGGTCCAG GCTCAGGAGC AGATCTTGAC CTCTGTTGCC 
AAGCAAGGCG AAGAGCTCCT CAGTCAGAGC CACCCTCAAG CTGGAGAGGT CTCCCAGAGG 
CTGGAGGCCC TGCGGGACCT CTGGGAGAAG CTGAGGCAGG CAGTGACCCT GCAGGGCCAG 
GCCCTAGAGA ACAGATACAA CTTCCAGGAG TTCCTGCAGA GAGTGGACCT AGCAGAGACC 
TGGATCCAGG AGAAGGAGAG GATGGTGAAC AGCTGTGACA TTGGCCTGAA CCTTGAGCAC 
TGCCTGCAGC TCTGCAGACA AGTCCGCAGG TTACAGGTCA CAGTGGATGA TGCCCACATC 
AAGGGAATCA AAAACTTGTC ACTGCAGCTC AAGAACCAGG GCCCTGAGGA ATCCGAGACC 
ATCTGCCAGC GGCAAAACCA GCTCAACAAT AGGTGGAAGA CTTTCCATGG CAACCTGCTC 
CTCTATCAGC AGCGGCTTGA AGCAGCCCTG GAGATACATA GATTGTCCAG GGAACTGGAT 
GACGTTATTG AGCGGCTCAG GGAGAAGGAA TCCCTGATCT GGGCCCCAGA GGGCACTGAA 
GATTTGGAGA ATGTGCAGAG GCTATCATGG AGACAGAAAG TGCTGCAGCA GGAGATGGGC 
CTCATCCAGA CCCAGGTGGA GTCTCTTGAA GGTAGGATTG GCCGGCTCTG CAAGGAAAGT 
CCCGAGGTTG CCCACAGCCT CAGACATAAG CAGCAAGAAA TGATGGACAG CTGGTGGAAG 
GTCTGGAGCA AAGCCCAGAA GTGGAGGGAG TTGCTGGATG TGGGCCATGA AGTTCAGAAA 
CTCCAGACCC TGCTGCAGGA TCTGCAGGAC TGGGCCCGGG GACTACAGGC TGAGATGGCC 
ACACAGGGCA CCCCCTGCAG CCCTGTGAGA ATCCAGTACA TGTTGGAAGA GTACCGGGCA 
TACAAGGTTG AGCTGGACAT CCGAACAGAG AGCCTTAACC TGGTCCAAAG CATGGGGCAG 
CGGCTGCTTG CATCTGGCTA CCCACAGGCC TCTGAGATTC ACCAGACCCT GGCCGCTGTA 
GAGCAGGGGT TGAGTAGCTT GCGGGAATCC TGGCAGGGCC GGCAACAGCA GCTACAGCAG 
GCCTTGGAGC AGCAGCTGTT TCTGGGCTCT GTGGAAAAGG CGGAACGCTG GCTTGACAGT 
GAAGAGGCCT CCCTGGCTAG TGAGGGAGTG GCGGACCCCT TGGTCACTGT GGAGACCCTT 
CTGTCAAAGC TCAAGAGGCA TGAGCAGGGC CTGAAGGCCC AGGCCGAAAA GATCCGTGCA 
TTGGAGGCCA CAGCCCACAG CCTGCACCAG GGTGGACACT CTGAGGCGCG TAGCATCCTG 
GACAGATGCC AGGCCCTGCT CCTGAGGACA GAGGCACTCA CAGAGCAAGC GAGGGCCCGG 
GGACACCAGC TGGAGGAGCT GCGGAAGCTT CGGACATTTT TGCAGGATTC CAATGAGGTA 
ACTGCATGGC TGAGGGAGAA GAGCCTGGCG GCTCTGGATG AGGGCCAGCA GGACCCAGCC 
ACGATGCAAA CACAGTTGCA GAAGCAGCAG AATTTCCAGG CTGAGCTGGA CGCCAGTGTT 
CACCAACAGC AGGAGGTCCA GATGGAGGGG CAGAAGCTGC TACAAGGGGG CCACCCGGCC 
TCAGAGACCA TCCGGGGGCA ACTGGAAGAA TTAGGAGGCC TCTGGGATGA GCTGCAGACC 
AACTGCCAGA GGAAGATGGC CAGACTACAG GGGGCCCTCA AGGTCCTGCA TCTGCAGAGA 
ATGCTGAAGG AACTGGAGAA GTGGCTGGAG CACATGGAAG CAGAGCTGAG AGTCCCTGTC 
AGAAGCCAGG CGCTGCCTAG AGTGGGCGAA CTCCTGGGAG CCCAGGAGGA GCTGGAAGCA 
GCCATGGACA GGCAGGCCAA GGAAGTGCAA GAGTTGCAGG GCCAGTCCCA AGCCTGCCTC 
CAGGAAGGCC ACTGCCTCGC CAAAGATGTG GAAGAGCAAG CCCGGCAGCT GCTTCAGAGG 
TTTCAGAGCC TTCGGGAGCC CCTACAAGAG CGCAGGGCAT CCCTGGAAGC CCAAAGGCTC 
CTCTTACAGT TCTTCAGGGA TGCTGATGAG GAAATGGCCT GGGTGCAGGA GAAGCTGCCC 
TCAGCCACAG CCCAGGACTA CGGCCAGAGC CTTAACACTG TGCGCCACCT GCAGGAGAAA 
CACCAGAATT TGGAGAATGA GATCCATAGC CACAAGGCTC TGAGCCAGGT GGTGACAGGT 
ACAGGGCACA AGCTCATACA GGCAGGGCAC TTTGCCACTG AAGAGGTGGC CGCCCGGGTG 
CAGCAGCTGG AGGTGGCTCT AAACCGCTTA GAGACAGAGG CCGCACAGAG GAGGAGGAGA 
CTGCAGCAGG CCCTGGAGGC CCAGCAGACT TTGGTGGAGC TCCTGGAAGC AGGATCCTGG 
CTGGCTGAAC GGGACCATAT TCTGGACAGT GAGGACCTGG GCCAGGACGC TGAGGCCACA 
CAGGCTCTTC TGCGGTGCCT AGAGGCCACC ACAAGAGACT TGGAAGGGTT CAGCAGTCGC 
ATTGAGCAGT TACAGCAAAC AGTTGCCCTT CTGGAGAGTG GACAGACCCC AGGCAGTCCT 
AGGGTGCTGG CCCAGCTGCA GGCTGTGAGA GAGGCCCATG CACGGCTGCT GCAGAGAGCA 
GAAAGCAGGG GGGAGGCGCT GCGTGAACAG CTGCACCTGT ACCAGCTGGA GCAAGAGGCC 
TTGCTCCTGG ATGCCTGGCT GACCACCAAG CTGGCCGTCG CTGAGTCTCA GGACTATGGA 
CAGGATCTAG CAGGCATCAA GGTGCTGGAA GACATGTTTG GTGCTTTCAA CAGAGAAGTC 
CAGAGCCTGG GCCAGGCCAA GATGCAGACC CTGAGGGAGC GGATGGCTTC CCTAGAAAGA 
GGAGCACCCA GGTTCTACCC CCAGATTCAA GCTCAGAAGT GTCGCGTCCA GGCCGCTTGG 
GAGGGGCTGA ACAAGGCAAT CAAAGTCCGT ACAGAGAACC TGGCAGCAGC CTGCGATCTC 
CGGAGCTTTG AGCAGGCTGC CTCGGAGCTG CAGAGATGGA TACAGGAGAA GACCACCCTG 
CTGGAGGAAG CATTCCAAGT CCACAGCCTG TCACCTTCAC AGCCCCTTCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC ATAGGCGCCT GCAGAGAGAA CTGAGGGCTA TTGAAAAAGA GGTGTCAAGA 
GTGCAGATGG AGGCCCATCG GCTCGGCCAG CACTACCCTG TGGCTCAGGG GAGTCTTAGT 
GAGTGGCTCA CCAAGGTGCA GGGGGCCTGG ACCAACCTGG AGGCCAAAGT CCAGGAATGG 
AGCCAGAAGC TGTTGCAGGC TACCCAGGGC CACACCTTTC TTGGGAGCTG CCGGGAATTG 
CTAGCGTGGG CTCAGGAGAT GCAGGAGCTG CTTTCTAAGG AGAAGCAGGC TGGGGATGTG 
GTGGGGGCCA AGCAGTTCCT TGAGCAGCAT GAGGCGCTGG AGCAAGAGAT TCAGGAACGC 
TGCCTTCAAG CCCAGACCAT ACGGCACGAA GGGCAGCAGC TGCTGGACAA CGGCCACTTC 
CTGTCCCCAG AGGTGGCAGA GTGTATGCAG GAGCTGGAAA GACATCTGCA GGAGCTTCAG 
GTAGCCTGGG CCCTGCGTGG ACAACGCTGG GAACAGACCC GGAGCCTGCA ACAGCTGCGG 
CAGAGGCTGG AGCTGGCTGA GGCTTGGCTG GCCTCCTGGG AGCGCCTGCT GCTGGACCCC 
AGCTGTGGGC ACTCGGTGTT GGAAGTGGAA CGCCTGCTCT ACAGACACGA GGGCTTAGAA 
AAGCTGCTGG TGGCCCACGA GGAGACATTC ATTCAGCTTC AGACGATGAC AGAGGAGGCA 
AAGGGTGGTC ATGTCACGGA GGCATCTGTG GAACAGCAGC CCCCCACAGA AGGGAGGCAG 
CCCAGAACCA GCTTCCCAAC CAGCAGCCAT GGGATCTTGG CAAGGGGAGC CTCGAGCCCA 
TTCTCAGATA TGAGGAAGGC AGAAGTGCCA AGTGGCACTG GGGAACTGGC TTACGTGTCC 
CCCCTTGTAC CTGAGGAGAC TGAATGTGAG AGGCTGGAGG ACAGAAGGCA GACTTTCTTC 
CCAAGAATCC CAAAGGAACC CTGGATTTTT CCACCTCCCT GCAGTGAGCT CTCAAGGAGT 
CTGCTTGGAA GTGTTGAGCG CTCACCCACA CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spectrin βV adaptive mutations and changes in subcellular location correlate with emergence of hair cell electromotility in mammalians.
Proceedings of the National Academy of Sciences of the United States of America114(8)2054-2059(2017 02)
Cortese M,Papal S,Pisciottano F,Elgoyhen AB,Hardelin JP,Petit C,Franchini LF,El-Amraoui A

The giant spectrin βV couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route.
Human molecular genetics22(18)3773-88(2013 Sep)
Papal S,Cortese M,Legendre K,Sorusch N,Dragavon J,Sahly I,Shorte S,Wolfrum U,Petit C,El-Amraoui A

alphaII-betaV spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells.
Journal of cell science121(Pt 20)3347-56(2008 Oct)
Legendre K,Safieddine S,Küssel-Andermann P,Petit C,El-Amraoui A

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Sptbn5 cDNA ORF clone, Mus musculus(house mouse)

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