SPTBN5 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTBN5
Entrez Gene ID	423225
Full Name	spectrin beta, non-erythrocytic 5
General protein information	Preferred Names spectrin beta, non-erythrocytic 5 Names spectrin beta chain, non-erythrocytic 5
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025151299.1	XP_025007067.1	spectrin beta chain, non-erythrocytic 5
XM_421149.5	XP_421149.5	spectrin beta chain, non-erythrocytic 5

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sptbn5	XP_006500600.1
Gallus gallus (chicken)	SPTBN5	XP_421149.4
Drosophila melanogaster (fruit fly)	kst	NP_995988.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004440	XP_313728.5
Bos taurus (cattle)	SPTBN5	XP_003586608.2
Rattus norvegicus (Norway rat)	Sptbn5	XP_006234865.1
Danio rerio (zebrafish)	LOC569585	XP_698075.4
Caenorhabditis elegans (roundworm)	sma-1	NP_001256383.1
Xenopus tropicalis (tropical clawed frog)	sptbn5	XP_004917485.1
Homo sapiens (human)	SPTBN5	NP_057726.4
Pan troglodytes (chimpanzee)	SPTBN5	XP_001142930.3

Gene Ontology

The following SPTBN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa36711	XM_421149.5 Latest version!	Gallus gallus spectrin, beta, non-erythrocytic 5 (SPTBN5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTBN5 ( XM_421149.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_421149.5 Gallus gallus spectrin, beta, non-erythrocytic 5 (SPTBN5), mRNA.

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CloneID	OGa36711
Clone ID Related Accession (Same CDS sequence)	XM_421149.5
Accession Version	XM_421149.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	spectrin beta chain, non-erythrocytic 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_421149.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGCTGC AAGCCAGGAT GTTTGGGTAT GAGATCTGCT CGATCTCCAC AGTCTCTACT 
TCTGGCTTAA GCAGAACAAG ATCAAGGCAG GTTGCTTTGC ATGAAAACCA TAAGCCGAAC 
GTAGGAGTAC AAGTGAATTT AGAACTGAAA GTCATTCTCC AGAATGACCA ACTGCTGAAC 
ACGAGAAAGG AGCTCCTCCT GACCTCAGTC ACCATGAATA GTGAATATGA AAAAGATCAC 
ATCAAAAGGC TGCAGGAGCA GCGTATGTTC ATGCAAAAGA AAACCTTCAC CAACTGGATG 
AATAATATCT TCTTCAGGAA TAATGTAGGG GCTGAAATAG AAAATATTTA CACAGACCTG 
AAGGATGGCA TCTATCTTAT GCAACTCCTG GAGCTGCTCT CTGGAGAATC TTTGCCCAGA 
CCGAACCGGG GAAAGATGAG AGTGCATTTT CTAGAGAACA ACAGCAAAGC CATTACATTT 
CTGAAATCCA AGCAGGTGCA AGTGAAAGTG ATTGGTCCTG AGAATATTGT AGACGGTGAC 
AGAACCTTAA TCCTGGGACT CATCTGGATA ATTATACTTA GATTTCAAAT TTCATCTATC 
AAACTTGATA AGGAAGAATT TGGAGGTAGA GCTGATCAGC TATTTGCCAA TGAAGCCCTA 
CTACTTTGGT GTCAGCATAA AACTGCCAGC TATCCCAACG TAAATGTGAA GGACTTCTCT 
AAAAGTTGGA GTGATGGACT GGCTTTCAAT GCACTTATAC ATGCCCACAG GCCTGATCTT 
ATCAATTATA GCTCACTGCG GCAGGACCAG CCCATCAAAA ACCTGAACAA TGCCTTTAAT 
GTTGCTGAGA AAGAGCTAGG GATCAGCAAG CTGCTTGATG CTGAGGATGT GGCAGTACCC 
TCCCCAGACG AGAGATCCAT CATGACTTAT GTGTCACTCT ATTACCACTA CTTCTCCAGA 
ATGAAACAAG GCCAGACAAA TCAAAAGCGA CTTGCCAAAA TTGTGTTCCT CCTGAAGGAG 
ATAGATGACT TGAAGCTTCT GTATGAGCAG ATGGTCTCTG ACCTCCTGAA ATGGATTAAG 
CAGAAGGTGG TTGAGCTGGA TGACCGTGTG TTCCCCAACT CTCTTCAGGA AATGTGGCTG 
CTCATGGCCA ATTTCAAGAC CTTCCGCACT GTGGAAAAAC CCCCTAAATA TCAAGATAAG 
GGCATGATTG AAGCTCATCT CTTCAATATC AGGACCAAGC AGAGAGCCAA CAACCTACGA 
CTATATGTGC CTCCAGAGGG GAGGACACTG CAGGATGTGG AAAAGCACTG GATTATCCTG 
GAAAAGGCAG AACACAACCG GGGAAAAGCA CTGCAGAAGG AGATGCTGAG GCTAGAGAAG 
TTGGAACAGC TAGCCAAGAG GTTTCTGAAG AAGGCAGCTC TGCGTGAGAC TTACTTGGAA 
GACATGAGGA AAGTAATTGG AAAGCAAGAC TTCTGGCCAG AGAGTGTAGA TAGGATGGAA 
GCTGCCAGTA AGAAGCTGGA AGCCATTGTG GCAGATGTAC TCCCCAGGAG GGAACGCTTC 
GCATCTTTGG CCGAAATGGC AACAGTCATC AGCCAGAATA ACTATCACAG CAAAGATCAA 
ATTGTGAAAA AGGAAAACAG CATTTCAAAG CAATGGCAAG ATCTTCTGGA TCAGCTGCAG 
AAACGACAAC ACTCCCTGAG TACAATGCAG GAGATTCTAG CCCTCCTGAG GGACATTGAT 
GCCATTATGG AGGAGCTGAA AGAGCTGCAG GCACTAGTGA ATTCTCAGGA CTGTGGGAAG 
CAGCTCCTAG AAGTAGTAGA TTTGCTTCAA AAGCACAACT TGATTGACTT GCAAATCTCT 
TCCTATGATG ATAAACTGAC ACACATAACT CAAAGGACAG CAGAAATCAG CAAGGATAGC 
ACAGTTAAAG CAGAAGTGCT TTATGCAAAA GTTCCGATGC TTCGTCAGCT ATATCAGAAC 
CTTATAGCTC AGAGCAGAAC ACGAAAATCC CAGCTAGAAG AGGCTTTGAA GCTCTTTGAA 
TTCTTCCGTG ACTGCAAAGA GGAAGAAGCA TGGATCTCTG AGAAGTGGAA AATAGCAAGA 
ACAACAACAT TAGGGAAAGA TGTCAGCCAG ATTACAGCTT CCATTCAGAA GCACAAGGCT 
CTTGAAGCAG AATGTAACTC GCACAGGGCC ATTTGCGCAG ATGTGATGAG GAGGGGCTGG 
GATCTGAGCC AGAAGAACCC TACACATGAG GAGAACATCC AGAAACAGAG AGACAAACTT 
CAAACATTGT GGCAGCAGCT CCAGGATGAG GTGGCTAATC GCAAAAACCG CCTGCAGGCA 
GCAGCTCTCA TCAAACAGTA CTTTGCTGAC ATCGATGAAG CAAATTCATG GTTGCAAGAA 
AGGCAAACCC TTGTGGCCAG CAAGGACTAT GGAAAAGATG AATCAAGTGC TGAAGCGCTG 
TTGCATCGTC ATTTGCGGCT GGAAAAGGAG ATTGCAGCCT ACTCCACAGA GATGAGGCGC 
CTGAAAGAGC AGGCTGCCAT TGCAGCAGAG CAAGCTCCAG CTGCAATGGT GAAGACAGAA 
GCCCCAGATG ACTTAAATCA GAAGACAGCT AAGCTACCAT TCAGTCGAAC CTGGGCTACC 
TCTGAATCTG CACTTCCTGG AAGCTCCAGT TTAGACATTC ATTTTGTTCC AGAGAACATT 
TGGAAGACCC AAGATGAAAT AGACTCACTT TATGAAAACC TACAGAGCAT GGCTGAGGAC 
AGAAAAAAGG CTTTGGAGGA GATGATTGAG TATTACCGCT TCTGTAGCTC CTGTGAAGCA 
TTTCAGTCAT GGATGAGAGA AAAGGAAAAC ATTTTTAGGA CTCTTCAGCC TCAAGCAGAC 
AACGTGGAAG TCATGCAACA AAAGTACCAG AGTTTTTTAA CAGAACTGGC GGCAGGGAAA 
GGCCAGCTGG GAGAAATTGA AAGCTTAGCT ACTAAATTTG GAAAGAACAG TCCTGGCAAA 
TACACTGAGA TCCAGATTTG GCTGAAAGAA ATCCATGCCA GGTGGCAACA TATGGAATCT 
CTGAAGGAGG AGAAGGGCTC AGAGCTGATC GGAGTGGCTG ATGTGAGGAC ATTCCTTGAA 
GACTGTCAGA GCATAGGAGT GCTACTGCAA GACAAGATGG GCCAGCTCAG GAATCTGGAA 
CCAGGGAACT CACCTGCTGG GCTGGAGTCT GACAAGCGCA AACTGTCTGT AGTGGAAAGA 
GAGATCCTAG TGATAGAGAG GAAAATTGAA TACTTGAGGA GTGTTGCAAA ATCCATTAAG 
GACACCAACC CTGCAGAGAG CAAGGCCATC ACAGTGCAGG TGGAGAACAT GGAAAGTCTT 
CTGGTAAACC TGAAGTTGGA GACCCAAAGG AAGCGTGACC TTCTGCAGCA GGCCCAAAAT 
CAACAGTCCT TTCTGCAAGA CAGCCGCAGG CTCCTGCTGT GGGCAGAAGG CATCAGGGAG 
AAGCTGAGAA GTGAAGAAAT GGGTGTGGAT GTGGCTTCTG CAGAGCATCT GCTGAGGGAA 
CATCAGGACC TTCTGAAAGA AATAAGGTCT CAGAAGGAAA GATTTCTACA GCTAGAAGAG 
CTTGGGCGTA AAATAATCCA AAAACAACCC AGTAACAGTA GGACCAGAGA TGTTCACCTG 
TCTGTGGAGA GGCTTGCCGA GGAGAACAAA GAGCTGGAAA ATATGTGGGA ACAGCGATGG 
AAAAAGTTGC AGGATGGCTT GGAATTGCAG AAGTTCAACA GGGAGGGAGA CCGTATCAAT 
GCTGCCCTTT CTAGCCATGA GGCTTTTCTC CGGGGTGATG ACCTTGGGGA TCATGTAGAT 
GCTGTTCGAA GCCTGCAGAA ACAACATCAG GAATTTGAAC AACTGTTAAT GGTGCTAAAG 
AGACGGATTG AAGCATTTAA TGAAAATGGG GCGGACCTAA TAGAAAGTGG GCACTTTGCA 
TCACATGCTA TTAAAGAGAG AATGGCTACA CTCCGGCAAA GATGGGAACG ACTGGTAGAG 
AACAATTCCA AGAGAAAACA GAGGTTGTTG GATTCCCTGC AGCTGCAGGA ATTCAACCGT 
GATGCTGCTG AGCTTTTGAT ATGGATGGAA GAGAAGTACA AGATTGCATC TGATGAATCC 
TATCGTGATC CAACAAATGT TTTACGCAAA CTGAAGTGGC ATGAGGCTGC TGAGAAGGAA 
ATGATGGCTA ATGAGAAGCA CTTCATAACA CTGATAAAGA AAGGAAATCA GCTGATTCAA 
GATGACCATT ATGCTGCAGC TTCTATCCAA AAGAAGATGT CAGAGCTAGA GAAGAAGTGG 
AAAGAATTGT ATGGGAAGAT GATAGAGCGA GGTGACAAGT TAAGACAAGC TGGGCAGCAA 
GAACAGCTCA TGGAACTGCT GCAGGATGCC AAAAAGAAGA TAGAGAAAAT TGAGAAGGTT 
CTTCAGGAAG CAGAGACTGG CCATGATTTG CGCTCCAGCC GTGATCTACT CAAGCAGCAC 
AGGCAGCTGG AAAATGAGAC ACACGAGCTG GCAGAGAAAA TGAACTCTAT TGTGTCTCAT 
GCAATGAAAA TGGCCACCAA CCACTTTGAC TCCCAGAGAA TTTTGGATGA AACGCAAAAA 
TACCTAAAAA GGTTTGAGTC TTTGCAAGCA CCTCTTGATG AGAGGCGTAA GCTGCTGGAA 
GCTGCAGTGG ATCTTTATGA GTTCTACCAT TACCATGACA TGGAGCTGAA CTGGATTAAT 
GAGCGACTGC CTATTGCCAG TTCCACCAAC TGTGGGAAGT CCTTGGATGT GGCTCAAAGT 
TTGTTGCAAA AACACAAGGA ATTTCAGGCA GAAGTGAATG CCCACAAGCA GCAAGTCCAG 
CGGGTCCTGG ATAAAGGAAG GACAATGATT GTAAACCAGC ATCCATCAGC TCAGAAAATA 
AATGAGAAAT GCCAGGAACT TGTGACTGCC TGGCAGGGCC TGGAGAAAGC CTGTGAGGAA 
AGGACAAAGC AGCTGCAGCA CTCAGTTGGT TTCCAAGAGT TTCTAATGAA TACTTCAGAC 
CTGGAGGCTT GGATAGCAGA AAAGCGTCCA CTTGTGATGA GCAAGGACTA TGGTAAGGAT 
GAAGATGGAA CATTGAAACT CCTCCAGAAA CATAAGGTGC TGGAGCACGA GATTGCTGTT 
TACCAAGATC TGATTAAAGA ACTAAATGAA AGTGTCCAAA ATCTTCCTGC CATGGGTTCC 
ATCCAGTACG TTGAGGTTGA TGCACCACGT GAACAAGTTC ACTCAAGACT CCGTGAGTTG 
CAGGAACTAG CAGCAACAAG AGGGAAGAAG CTGGATGAGA CTCTTGTCTT GCATGAATTC 
CTACGAGAAT ATGAAGATCT GCAGGACTGG ATGGCTCAGC AGAAACAAGC AGCCAGCTCT 
GAAGACTATG GAAATAATTA TGAGCATGTC TTGCAACTTC GTGCCAAATA TGACACTTTC 
CGACACCAAT TGGAAGCAAC TGGGAAAAGA GTTATGACTT GCCAGCAGCT GGCAGAGAAC 
TTGCTAAACG CTGGTCATTC TGAGTCCCGT GAAATCCGGC AGAAGCAGAA AGACCTAAGA 
AACTCTTGGG AGGAGCTGTT AGAAATTACC AGATTACGCG GTGAGCGGCT GAAAGATGCT 
GAGGTGATTC ACAAGTGTTA CCAAGATCTG ACAGATGCTC TGGCCCACAT TGAGGAGAAG 
TCCAAAAGTA TTCCAGATGA TGTTGCGAAG GACATGAGAG GTGTGCAAAC CCAGCTCCGG 
AACCATGTGG CCCTGGAACA TGAGCTCCTT GGAAATGAGC AGCAGCTGCA GGATCTGATC 
CACTCTGCAG ATCATGTGCT GACTCGTTGC TCAAATGAGC AGGCGTCAGG CCTGAAAGCA 
AAGCAACAGG CAATAGTGAC TAACTGGAAG GCCCTGAAGT CTAAAGTGCA ACTGCGTAAG 
GAGCTTCTGG AACAAGCACA CAAGCTTTAC CAGTTTCAGG CACAAGTGTG GGACTATTTC 
TCATGGACAT CAGAAATGAT TAGGGAAATG AGAGCCAAGG AGACTATCCG GGACATATCT 
ACTAGCAGCC TGAGACTTAC CCAGCATCAA CAGCTACTGG CTGAGATTGA AGCACAAGAA 
GAGAAGTATA ATCAAATTGT ACAGCTTGGA CAGTCACTCT TGCAAGATGA GGAAATAAGA 
TCAAAAGAGA TTCGTCAGAA GCTACAAGCT CTGTTGGAAG AGAAGGACAA AGTATACAGT 
GAATGGCAAC AGAAGAAGAA GTGGCTGGAC AGAATACATC AAGAACAAAT GTTCTACAAG 
GATTGGAATC ACCTGGACAA GCTCCTGAAC TCGCAGGAGG TTTATTTGAA AAGCAGTGAC 
CTTGGAAGCT CTGTAGATGA AGTAGAGCAA CTGATTAGGA AACATGAGGC ATTTGAGAAG 
CTACTGGCAT CACAGGATGA GAAGATAACG TCTCTTCAAG AACAAGCAAA CAAGCTAGAA 
AAGGATGGTG GATTGGATGG GGTACAGATT CAGCACAAGC TGGACACCAT TCTTCAGAGG 
AAGAAGCAGA TCAAGAATCT CTCCCGGAGC AGGAGAGAAA AGCTGCAGAC CGCCCTTCTT 
CTGGCACTTT TCTATCAGAA CTTGGCAGAG GCAGAAGACT GGATCAGTGA GCGCATGCAG 
AAGCTAGAGG ATCCTTCCAT ACAAGATCCC AGCAATCTGC AGGACAAAAT GAAGCTGCTG 
CAGAAGCATC AGGTCTTTGA GGCAGAGATT CTAGCAAATG AAGAAATCAT CACAGATGTT 
AATAAGAAAG GAGAAGCCCT GGTTTCAATG GGCCACCCAA AATCGGGGGA GATCCGCCGC 
CAAGTCCGCA TGCTTCAGGA GCGCTGGGAG AAGTTGAAGA GAGCTGTTGC TGCCCGTGGA 
AAGATGCTGG AGGATAGTCG AGATTTCCTG GAATTTCTCC AGAAAGTGGA CCAGGTGGAA 
GCCTGGATCA GGGAAAAGGA AGTCATGATT AACATAGGTG ATGTGGGAAA TGACTATGAA 
CACTGTCTGC AGCTCATCAG AAAGCTGAAT GAGTTCCGTG GAGCAACATC AGGGGAGACA 
ACAGTGGATG ATGCTCACAT CAAGGCTATC AATGCCCTGG CCATGAAACT GGAGAGACAA 
AACAAGGAAG AGACAAAGAC AATATATCAG CGCCGGAAGC AGCTCAATGA GAAGTGGAAC 
AGTTTCCATG GTAACCTGAA TGCATACAGG AGGAAGTTAG AAGGAGCTCT GGAGATTCAT 
GCCTTGATCA GAGAAATCGA TGACATCACA GAAAGAATTA CTGAGAAGAG TGTCCTGATA 
CAAGCACTGG ATTATGGGAA AGATGTTGAA AGTGTGGAAA ACCTTATTCG AAGACATGAA 
GAGATGGAGA GAGAAATCAA TGTTATTAAG TCCAAAATAG AGCCATTGGA ATTGGAATCT 
TTCCGCCTTT CCACAAGGAA TCCATCCATA AATGACAAGC TGACTATGAA GCAACAGGAG 
ATGAAAAACA ACTGGCTACG ACTCCAGGGA CAGGCCAAAC AAAGGAAGGA GAAGCTGGCA 
GCCTCATATC AGTTGCAGAA GTTTAACTCT GAGATGAAGG AAATTACAGA TTGGATTCAA 
AACATCAGAG GCTTAATGGA AGCAGGGGGG CTTCCTAAAA GCCTAAATGA AGCAGAAAGC 
ATGATTGAGG AGCATCAGCA GAGAAAGGAG GAGATTGAAG CACGAGTGGA AAGATTCAAC 
TCTCTCAGTA ACTATGGTCA ACAACTTGCC AATTCTGGTC ACTACGCAAC CCCTGAGATT 
CATCAGTCTC TCTCCAGACT ACAGAAAGCC TGGACTGAAT TGATCCAAGC ATGGCAGGAG 
CAATATATAA AATTGTTTCA AGCACAAGAT TTACAGAAAT TCTATGGCTA TGTGGAACAG 
ACCGAGAGCT GGCTCAGCAG CAAAGAGGCT TTCCTAGCCA ATGAAGACTT AGGGGACTCA 
GTGTCTAGTG TGGAGAGCCT GCAACGGAAA CACATGCAAT TTGAAAAATC CCTGGAAGCT 
CAGATGGAGA AAATTGATGA GATGGCTTCA TTTGCCCAGC AGCTAATTCA GAACAAACAT 
TATGACTCGG ACAACATCAC CAACAGATGC CAGGCCGTTC TGAGGAGAAA AGAGAAATTA 
CTGGAGAATG CTGCTGCTCG CAGACATCTA CTAGAGGAAT CAAGGCTTCT GCAGAAGTTG 
TTGAAGAATT CCTATGAGGT GGCTACCTGG ATCAATGAGA AGAACAGTAT TGCTCAGGAT 
GATAGCTGGA GGGATCCAAG CAATCTACAA ACCAAGCTGC AGAAACATCA AAGTTTCCAA 
GCAGAGATCA TGGCCAATAA AAATCGCCTG GACTCCATCA AATCTGAAGG AGAGAAGATG 
ATCCGTGAGA GACACTATGC CCCTGAGGCC ATTCAGTCTA GGCTACAAGA AATGGAAGAG 
CTATGGGAGG AACTGCTAGC AAGCTGCCAA GATAAACGAG CCAAGCTGCA GGATGCTTAC 
AAGGCCCTTC ATTTTCAGCG GAGTATAGAG GATACAGAAA AATGGTTGGA AGATGTGGAA 
AATGAGTTGA AAGCACCATA TAGCAGCGAT GATCTAGTGG TTCTGAACAG TCACCTGAAG 
AAACAAGAGG AACTGGAGCA AGATATTGCT AGCCATAGGG ACCGGTTGCA AGAGCTTGTG 
AACACAGCTC AGGAATTCCA GCAGGAAAAG CATTTCCTTG CCGATGAGCT AGAAGAGAGG 
GTCGATCAGC TGGTGCAAAG ATACAAAAGT CTACGTGATC CTCTGCAGGA GAGACGCGGG 
AGTTTGGAGG CAAGCAGACT TCAGTACGAG TTCTTCCGAG ATGTTGATGA GGAGTTGGCC 
TGGGTTCATG AGAAACTTCC CATGGCATCC TCCAGGGATT ACGGCCAATC CCTGGCAACT 
GTTCAGAGTC TACAGGAAAA GCACCAGAAT TTGGAGAATG AGATAAGCAG TAGGGATGCC 
TTGACCAAAG CAGTGCTCAG TACTGGGCAG AAACTGGTAA GGGGAGGCCA CTCAGCCTCT 
CGCAAGATTA TGGAGCAGAT GAAGGAGCTG GAGACATCTG TAGAAAACTT GAAAGCAGAG 
GCTCATGTGA GGAGACAGCG TCTCATGCAG TCATATGAGG CCCATCAGTT CCTGACTGAG 
CTACTGGATG TGGAGACCTG GCTGGCAGAG AGGAGCTTCA TTCTGGAGAC TTCTGATTAT 
GGGAAGAATG AAGAATCCAC TCAAGCTTTA CTACGTAAAC TTGAAGCCAC CAAGCTGGAC 
ATGGAGGGTT TTAGGCCCCG GATTGAGAAA GTACAGGAGA CAGGTGCCAG CTTAATAAAC 
AAAGACAACC CAGAGAGTCC AGCCATACTT TCAAAGCTCC AGAGGATCCT AGCAGACTAT 
CAGTCTCTCC TACAAAAGGC TGAGACCCAG AGAAAACGCC TGCAAGAACA ATTCCAGCTG 
TATCAGTTTG AAAGAGAATT CCAGCTGGTT GACGCCTGGC TTTCCAGTAA ACAGTCTATT 
GCAGAGTCCG ATGACTATGG GCAGGACTTA GATGGTGTTG AGGTGCTGGA GAAAAAGTTT 
AAAGATTTTG TAAATGAGAT TAAACCTCTG GGACATTCCA AAGTTCTCTC CTTAAATGAA 
TTAGCATCTA AACTAGAGAA GGATGGCCAC AGCAAGACAG ATATTATCCA GAAAAGGACA 
AAACAAATTA ACGAGATGTG GGAGAAACTG TGCCATGCCA TCCAGACAAG GACAGAGAAT 
CTGAGAGCAG CCCAGCAAGT TCACCAGTAT GACCATGATG TGGATGAAGT AAAGGGCTGG 
ATGCAGGAAA AGGAGGCTGT GGTGGACATA GATGATTATG GATATGATCT TCCAGGTGTA 
CAAACTCTTC TGAGCCAGCT TGAAGGAGTG GAGAGAGATC TAGGAGCCAT TATGAAAGAG 
CTGGAACGGA TTCGAGGAGA AGCCTGGCAC CTCAGCCGCA CATACCCACA GCTCAAGGAA 
AACATTATGG AGAGACTCAC AGATGTGGAT GAGTGCTGGA GAAACCTGGA TAAGAAGTTT 
CTGGAGAGAA AGGCGAGACT GAGCCAGGCA GAACAAGTCC AGCTCTACTT CAATGACTGC 
AGGGAGCTAA TGGCATGGGC CAATGAGATG CACGCACTAG TGATATCTGA GGAACTAGCA 
AATGATGTCC TGGGAGCTGA ACTGCTTATC AAACGTCATG ATGAATACAA GCGTGAGATA 
GAGAAGCAGT GGCTCAAATA TGAAGAAATA CAGCGGGCAG GAGATGACCT GACAAGAAAT 
GGACATTTCA TGTCTGTGGA GATTGAAGAA AAACTGCTAG AGCTGTCAGA GCTGATGAAG 
AAAGTAAAGG AGAGCTGGAA CGTGAGGAAA GAGCTGTATG AAGAAAACTG GGAGATTCAA 
CTGCTCCGCA GAGAACTAGA ACAAGCAGAA GCATGGCTGG CTGCCAAGGA GAGCTTTCTT 
TCAGACCCTA GCTATGGGGA TTCTGTGTCT GAAGTAGAGG AACTACTGAA GAAACATCAT 
GACTTTGAGA ACATGCTTGC AGCTCAGGAG GAGAAATTTG CTCAGCTCAG CAGGAAAACT 
AAGAGGGAGA TGAATCTTCT GAAACAGACA GATGCAGAAG AAAGTGAGCA GAAGGATAAA 
GGCAAAATTG TACGGGTTCC TTCTCTGAAA AGAAAAACAT CTGACAAAAG GAATCCACCA 
CCAAAAGTGA TAGAGATAAG GAATACAAAG CCACTGGAAT CTGTGCAACC ACCACTCCTG 
TCCTGGACTC CACTTGAAAC AATTTTCTCT CCAGTTGAAA AGCCTGCAGT GGCATTCCAA 
CAATTTGCTT CTGGGAAAGT CCCTGAGGTG CTGAGCAGCA AAAAACCAGA AATGAAACCT 
GAGAGGAGAC TCAGTGAGAC TATCACTCCA CCTACACCAA AGACAGTAGA CCCACAAGCT 
ACATCTTTGA CACATCACAG TTCTTTAACC TCTCCTTTAT CTCCTACTCC CATAAGCCTG 
CAACGCAGCA GCAATTTCTC AGAATCATAT GCCACAGGCC TTTTATCACC TCAAGAAAAA 
AGTGCTGAAA GCTCCTCCTT CTCCAACCTG CAAACAAAAT TAGCAGCAAC ATCACCCAAG 
CCTGACCAGA AGTCATTTGA TAAATCACCA GATTTACTGC TGTCTAGGTC CTCACGGGAG 
ACATCGGAGA CTGCACCCTT GGTTTCTGCA AGTCTCCAGC ATATGGAGGG GTTCCTAGAA 
AAGAGAGACC AGCTTCTTCC AGGACGACAA CAGCCAGGCT CAAGGTCTTG GAAATCCTTT 
TATGTAAAAC TTGATGGACT ACAGCTTGAT TTCTACAATG ATGAACAAGA AGCATCTAAG 
AATATATCCA CACTCCTGTC CCTCAGCATT GTTGGTGCCA AATGTGAGAG GCTGACAAAC 
TACATCAGGA AAGAGAACAC CTTCATGTTA CGACTGAGAG ACGGGGCAGA GTATTTCTTT 
GCAGCACCTT CTCCGATGCT GATGGAGAAC TGGTTGCAGT CACTGCAGAA TAATATAGGA 
CATAGAAACA GGCCAGATTC TACAGTGATG GTGAGGCCTT TCACTCCAAG CACTGAGCCC 
TCTCAGACAG ATCTGTGGGA CAGAGACTCT GCTGAAAGAC TAACCAGCAA AAGTACACTC 
CTAAGGAGAA CACCATCTTT CAGAATCAAA CCAGAGAAGG AATCTGCAGC ATTTTTCAGA 
AATTTAAAGG AAGATGGGAC TGCAGTGACC CAAGCCTCCA CCACCACTCT GAACCTAGAA 
CAAGGTGGTA ATAACACAGA ACTGCCTCCA TCTCTACTAG AAGCAGGAAG AGAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_421149.5
CDS	1..11880
Translation	MELQARMFGYEICSISTVSTSGLSRTRSRQVALHENHKPNVGVQVNLELKVILQNDQLLNTRKELLLTSVTMNSEYEKDHIKRLQEQRMFMQKKTFTNWMNNIFFRNNVGAEIENIYTDLKDGIYLMQLLELLSGESLPRPNRGKMRVHFLENNSKAITFLKSKQVQVKVIGPENIVDGDRTLILGLIWIIILRFQISSIKLDKEEFGGRADQLFANEALLLWCQHKTASYPNVNVKDFSKSWSDGLAFNALIHAHRPDLINYSSLRQDQPIKNLNNAFNVAEKELGISKLLDAEDVAVPSPDERSIMTYVSLYYHYFSRMKQGQTNQKRLAKIVFLLKEIDDLKLLYEQMVSDLLKWIKQKVVELDDRVFPNSLQEMWLLMANFKTFRTVEKPPKYQDKGMIEAHLFNIRTKQRANNLRLYVPPEGRTLQDVEKHWIILEKAEHNRGKALQKEMLRLEKLEQLAKRFLKKAALRETYLEDMRKVIGKQDFWPESVDRMEAASKKLEAIVADVLPRRERFASLAEMATVISQNNYHSKDQIVKKENSISKQWQDLLDQLQKRQHSLSTMQEILALLRDIDAIMEELKELQALVNSQDCGKQLLEVVDLLQKHNLIDLQISSYDDKLTHITQRTAEISKDSTVKAEVLYAKVPMLRQLYQNLIAQSRTRKSQLEEALKLFEFFRDCKEEEAWISEKWKIARTTTLGKDVSQITASIQKHKALEAECNSHRAICADVMRRGWDLSQKNPTHEENIQKQRDKLQTLWQQLQDEVANRKNRLQAAALIKQYFADIDEANSWLQERQTLVASKDYGKDESSAEALLHRHLRLEKEIAAYSTEMRRLKEQAAIAAEQAPAAMVKTEAPDDLNQKTAKLPFSRTWATSESALPGSSSLDIHFVPENIWKTQDEIDSLYENLQSMAEDRKKALEEMIEYYRFCSSCEAFQSWMREKENIFRTLQPQADNVEVMQQKYQSFLTELAAGKGQLGEIESLATKFGKNSPGKYTEIQIWLKEIHARWQHMESLKEEKGSELIGVADVRTFLEDCQSIGVLLQDKMGQLRNLEPGNSPAGLESDKRKLSVVEREILVIERKIEYLRSVAKSIKDTNPAESKAITVQVENMESLLVNLKLETQRKRDLLQQAQNQQSFLQDSRRLLLWAEGIREKLRSEEMGVDVASAEHLLREHQDLLKEIRSQKERFLQLEELGRKIIQKQPSNSRTRDVHLSVERLAEENKELENMWEQRWKKLQDGLELQKFNREGDRINAALSSHEAFLRGDDLGDHVDAVRSLQKQHQEFEQLLMVLKRRIEAFNENGADLIESGHFASHAIKERMATLRQRWERLVENNSKRKQRLLDSLQLQEFNRDAAELLIWMEEKYKIASDESYRDPTNVLRKLKWHEAAEKEMMANEKHFITLIKKGNQLIQDDHYAAASIQKKMSELEKKWKELYGKMIERGDKLRQAGQQEQLMELLQDAKKKIEKIEKVLQEAETGHDLRSSRDLLKQHRQLENETHELAEKMNSIVSHAMKMATNHFDSQRILDETQKYLKRFESLQAPLDERRKLLEAAVDLYEFYHYHDMELNWINERLPIASSTNCGKSLDVAQSLLQKHKEFQAEVNAHKQQVQRVLDKGRTMIVNQHPSAQKINEKCQELVTAWQGLEKACEERTKQLQHSVGFQEFLMNTSDLEAWIAEKRPLVMSKDYGKDEDGTLKLLQKHKVLEHEIAVYQDLIKELNESVQNLPAMGSIQYVEVDAPREQVHSRLRELQELAATRGKKLDETLVLHEFLREYEDLQDWMAQQKQAASSEDYGNNYEHVLQLRAKYDTFRHQLEATGKRVMTCQQLAENLLNAGHSESREIRQKQKDLRNSWEELLEITRLRGERLKDAEVIHKCYQDLTDALAHIEEKSKSIPDDVAKDMRGVQTQLRNHVALEHELLGNEQQLQDLIHSADHVLTRCSNEQASGLKAKQQAIVTNWKALKSKVQLRKELLEQAHKLYQFQAQVWDYFSWTSEMIREMRAKETIRDISTSSLRLTQHQQLLAEIEAQEEKYNQIVQLGQSLLQDEEIRSKEIRQKLQALLEEKDKVYSEWQQKKKWLDRIHQEQMFYKDWNHLDKLLNSQEVYLKSSDLGSSVDEVEQLIRKHEAFEKLLASQDEKITSLQEQANKLEKDGGLDGVQIQHKLDTILQRKKQIKNLSRSRREKLQTALLLALFYQNLAEAEDWISERMQKLEDPSIQDPSNLQDKMKLLQKHQVFEAEILANEEIITDVNKKGEALVSMGHPKSGEIRRQVRMLQERWEKLKRAVAARGKMLEDSRDFLEFLQKVDQVEAWIREKEVMINIGDVGNDYEHCLQLIRKLNEFRGATSGETTVDDAHIKAINALAMKLERQNKEETKTIYQRRKQLNEKWNSFHGNLNAYRRKLEGALEIHALIREIDDITERITEKSVLIQALDYGKDVESVENLIRRHEEMEREINVIKSKIEPLELESFRLSTRNPSINDKLTMKQQEMKNNWLRLQGQAKQRKEKLAASYQLQKFNSEMKEITDWIQNIRGLMEAGGLPKSLNEAESMIEEHQQRKEEIEARVERFNSLSNYGQQLANSGHYATPEIHQSLSRLQKAWTELIQAWQEQYIKLFQAQDLQKFYGYVEQTESWLSSKEAFLANEDLGDSVSSVESLQRKHMQFEKSLEAQMEKIDEMASFAQQLIQNKHYDSDNITNRCQAVLRRKEKLLENAAARRHLLEESRLLQKLLKNSYEVATWINEKNSIAQDDSWRDPSNLQTKLQKHQSFQAEIMANKNRLDSIKSEGEKMIRERHYAPEAIQSRLQEMEELWEELLASCQDKRAKLQDAYKALHFQRSIEDTEKWLEDVENELKAPYSSDDLVVLNSHLKKQEELEQDIASHRDRLQELVNTAQEFQQEKHFLADELEERVDQLVQRYKSLRDPLQERRGSLEASRLQYEFFRDVDEELAWVHEKLPMASSRDYGQSLATVQSLQEKHQNLENEISSRDALTKAVLSTGQKLVRGGHSASRKIMEQMKELETSVENLKAEAHVRRQRLMQSYEAHQFLTELLDVETWLAERSFILETSDYGKNEESTQALLRKLEATKLDMEGFRPRIEKVQETGASLINKDNPESPAILSKLQRILADYQSLLQKAETQRKRLQEQFQLYQFEREFQLVDAWLSSKQSIAESDDYGQDLDGVEVLEKKFKDFVNEIKPLGHSKVLSLNELASKLEKDGHSKTDIIQKRTKQINEMWEKLCHAIQTRTENLRAAQQVHQYDHDVDEVKGWMQEKEAVVDIDDYGYDLPGVQTLLSQLEGVERDLGAIMKELERIRGEAWHLSRTYPQLKENIMERLTDVDECWRNLDKKFLERKARLSQAEQVQLYFNDCRELMAWANEMHALVISEELANDVLGAELLIKRHDEYKREIEKQWLKYEEIQRAGDDLTRNGHFMSVEIEEKLLELSELMKKVKESWNVRKELYEENWEIQLLRRELEQAEAWLAAKESFLSDPSYGDSVSEVEELLKKHHDFENMLAAQEEKFAQLSRKTKREMNLLKQTDAEESEQKDKGKIVRVPSLKRKTSDKRNPPPKVIEIRNTKPLESVQPPLLSWTPLETIFSPVEKPAVAFQQFASGKVPEVLSSKKPEMKPERRLSETITPPTPKTVDPQATSLTHHSSLTSPLSPTPISLQRSSNFSESYATGLLSPQEKSAESSSFSNLQTKLAATSPKPDQKSFDKSPDLLLSRSSRETSETAPLVSASLQHMEGFLEKRDQLLPGRQQPGSRSWKSFYVKLDGLQLDFYNDEQEASKNISTLLSLSIVGAKCERLTNYIRKENTFMLRLRDGAEYFFAAPSPMLMENWLQSLQNNIGHRNRPDSTVMVRPFTPSTEPSQTDLWDRDSAERLTSKSTLLRRTPSFRIKPEKESAAFFRNLKEDGTAVTQASTTTLNLEQGGNNTELPPSLLEAGREK

Target ORF information:

RefSeq Version	XM_421149.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus spectrin, beta, non-erythrocytic 5 (SPTBN5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_421149.5

ORF Insert Sequence:

ATGGAGCTGC AAGCCAGGAT GTTTGGGTAT GAGATCTGCT CGATCTCCAC AGTCTCTACT 
TCTGGCTTAA GCAGAACAAG ATCAAGGCAG GTTGCTTTGC ATGAAAACCA TAAGCCGAAC 
GTAGGAGTAC AAGTGAATTT AGAACTGAAA GTCATTCTCC AGAATGACCA ACTGCTGAAC 
ACGAGAAAGG AGCTCCTCCT GACCTCAGTC ACCATGAATA GTGAATATGA AAAAGATCAC 
ATCAAAAGGC TGCAGGAGCA GCGTATGTTC ATGCAAAAGA AAACCTTCAC CAACTGGATG 
AATAATATCT TCTTCAGGAA TAATGTAGGG GCTGAAATAG AAAATATTTA CACAGACCTG 
AAGGATGGCA TCTATCTTAT GCAACTCCTG GAGCTGCTCT CTGGAGAATC TTTGCCCAGA 
CCGAACCGGG GAAAGATGAG AGTGCATTTT CTAGAGAACA ACAGCAAAGC CATTACATTT 
CTGAAATCCA AGCAGGTGCA AGTGAAAGTG ATTGGTCCTG AGAATATTGT AGACGGTGAC 
AGAACCTTAA TCCTGGGACT CATCTGGATA ATTATACTTA GATTTCAAAT TTCATCTATC 
AAACTTGATA AGGAAGAATT TGGAGGTAGA GCTGATCAGC TATTTGCCAA TGAAGCCCTA 
CTACTTTGGT GTCAGCATAA AACTGCCAGC TATCCCAACG TAAATGTGAA GGACTTCTCT 
AAAAGTTGGA GTGATGGACT GGCTTTCAAT GCACTTATAC ATGCCCACAG GCCTGATCTT 
ATCAATTATA GCTCACTGCG GCAGGACCAG CCCATCAAAA ACCTGAACAA TGCCTTTAAT 
GTTGCTGAGA AAGAGCTAGG GATCAGCAAG CTGCTTGATG CTGAGGATGT GGCAGTACCC 
TCCCCAGACG AGAGATCCAT CATGACTTAT GTGTCACTCT ATTACCACTA CTTCTCCAGA 
ATGAAACAAG GCCAGACAAA TCAAAAGCGA CTTGCCAAAA TTGTGTTCCT CCTGAAGGAG 
ATAGATGACT TGAAGCTTCT GTATGAGCAG ATGGTCTCTG ACCTCCTGAA ATGGATTAAG 
CAGAAGGTGG TTGAGCTGGA TGACCGTGTG TTCCCCAACT CTCTTCAGGA AATGTGGCTG 
CTCATGGCCA ATTTCAAGAC CTTCCGCACT GTGGAAAAAC CCCCTAAATA TCAAGATAAG 
GGCATGATTG AAGCTCATCT CTTCAATATC AGGACCAAGC AGAGAGCCAA CAACCTACGA 
CTATATGTGC CTCCAGAGGG GAGGACACTG CAGGATGTGG AAAAGCACTG GATTATCCTG 
GAAAAGGCAG AACACAACCG GGGAAAAGCA CTGCAGAAGG AGATGCTGAG GCTAGAGAAG 
TTGGAACAGC TAGCCAAGAG GTTTCTGAAG AAGGCAGCTC TGCGTGAGAC TTACTTGGAA 
GACATGAGGA AAGTAATTGG AAAGCAAGAC TTCTGGCCAG AGAGTGTAGA TAGGATGGAA 
GCTGCCAGTA AGAAGCTGGA AGCCATTGTG GCAGATGTAC TCCCCAGGAG GGAACGCTTC 
GCATCTTTGG CCGAAATGGC AACAGTCATC AGCCAGAATA ACTATCACAG CAAAGATCAA 
ATTGTGAAAA AGGAAAACAG CATTTCAAAG CAATGGCAAG ATCTTCTGGA TCAGCTGCAG 
AAACGACAAC ACTCCCTGAG TACAATGCAG GAGATTCTAG CCCTCCTGAG GGACATTGAT 
GCCATTATGG AGGAGCTGAA AGAGCTGCAG GCACTAGTGA ATTCTCAGGA CTGTGGGAAG 
CAGCTCCTAG AAGTAGTAGA TTTGCTTCAA AAGCACAACT TGATTGACTT GCAAATCTCT 
TCCTATGATG ATAAACTGAC ACACATAACT CAAAGGACAG CAGAAATCAG CAAGGATAGC 
ACAGTTAAAG CAGAAGTGCT TTATGCAAAA GTTCCGATGC TTCGTCAGCT ATATCAGAAC 
CTTATAGCTC AGAGCAGAAC ACGAAAATCC CAGCTAGAAG AGGCTTTGAA GCTCTTTGAA 
TTCTTCCGTG ACTGCAAAGA GGAAGAAGCA TGGATCTCTG AGAAGTGGAA AATAGCAAGA 
ACAACAACAT TAGGGAAAGA TGTCAGCCAG ATTACAGCTT CCATTCAGAA GCACAAGGCT 
CTTGAAGCAG AATGTAACTC GCACAGGGCC ATTTGCGCAG ATGTGATGAG GAGGGGCTGG 
GATCTGAGCC AGAAGAACCC TACACATGAG GAGAACATCC AGAAACAGAG AGACAAACTT 
CAAACATTGT GGCAGCAGCT CCAGGATGAG GTGGCTAATC GCAAAAACCG CCTGCAGGCA 
GCAGCTCTCA TCAAACAGTA CTTTGCTGAC ATCGATGAAG CAAATTCATG GTTGCAAGAA 
AGGCAAACCC TTGTGGCCAG CAAGGACTAT GGAAAAGATG AATCAAGTGC TGAAGCGCTG 
TTGCATCGTC ATTTGCGGCT GGAAAAGGAG ATTGCAGCCT ACTCCACAGA GATGAGGCGC 
CTGAAAGAGC AGGCTGCCAT TGCAGCAGAG CAAGCTCCAG CTGCAATGGT GAAGACAGAA 
GCCCCAGATG ACTTAAATCA GAAGACAGCT AAGCTACCAT TCAGTCGAAC CTGGGCTACC 
TCTGAATCTG CACTTCCTGG AAGCTCCAGT TTAGACATTC ATTTTGTTCC AGAGAACATT 
TGGAAGACCC AAGATGAAAT AGACTCACTT TATGAAAACC TACAGAGCAT GGCTGAGGAC 
AGAAAAAAGG CTTTGGAGGA GATGATTGAG TATTACCGCT TCTGTAGCTC CTGTGAAGCA 
TTTCAGTCAT GGATGAGAGA AAAGGAAAAC ATTTTTAGGA CTCTTCAGCC TCAAGCAGAC 
AACGTGGAAG TCATGCAACA AAAGTACCAG AGTTTTTTAA CAGAACTGGC GGCAGGGAAA 
GGCCAGCTGG GAGAAATTGA AAGCTTAGCT ACTAAATTTG GAAAGAACAG TCCTGGCAAA 
TACACTGAGA TCCAGATTTG GCTGAAAGAA ATCCATGCCA GGTGGCAACA TATGGAATCT 
CTGAAGGAGG AGAAGGGCTC AGAGCTGATC GGAGTGGCTG ATGTGAGGAC ATTCCTTGAA 
GACTGTCAGA GCATAGGAGT GCTACTGCAA GACAAGATGG GCCAGCTCAG GAATCTGGAA 
CCAGGGAACT CACCTGCTGG GCTGGAGTCT GACAAGCGCA AACTGTCTGT AGTGGAAAGA 
GAGATCCTAG TGATAGAGAG GAAAATTGAA TACTTGAGGA GTGTTGCAAA ATCCATTAAG 
GACACCAACC CTGCAGAGAG CAAGGCCATC ACAGTGCAGG TGGAGAACAT GGAAAGTCTT 
CTGGTAAACC TGAAGTTGGA GACCCAAAGG AAGCGTGACC TTCTGCAGCA GGCCCAAAAT 
CAACAGTCCT TTCTGCAAGA CAGCCGCAGG CTCCTGCTGT GGGCAGAAGG CATCAGGGAG 
AAGCTGAGAA GTGAAGAAAT GGGTGTGGAT GTGGCTTCTG CAGAGCATCT GCTGAGGGAA 
CATCAGGACC TTCTGAAAGA AATAAGGTCT CAGAAGGAAA GATTTCTACA GCTAGAAGAG 
CTTGGGCGTA AAATAATCCA AAAACAACCC AGTAACAGTA GGACCAGAGA TGTTCACCTG 
TCTGTGGAGA GGCTTGCCGA GGAGAACAAA GAGCTGGAAA ATATGTGGGA ACAGCGATGG 
AAAAAGTTGC AGGATGGCTT GGAATTGCAG AAGTTCAACA GGGAGGGAGA CCGTATCAAT 
GCTGCCCTTT CTAGCCATGA GGCTTTTCTC CGGGGTGATG ACCTTGGGGA TCATGTAGAT 
GCTGTTCGAA GCCTGCAGAA ACAACATCAG GAATTTGAAC AACTGTTAAT GGTGCTAAAG 
AGACGGATTG AAGCATTTAA TGAAAATGGG GCGGACCTAA TAGAAAGTGG GCACTTTGCA 
TCACATGCTA TTAAAGAGAG AATGGCTACA CTCCGGCAAA GATGGGAACG ACTGGTAGAG 
AACAATTCCA AGAGAAAACA GAGGTTGTTG GATTCCCTGC AGCTGCAGGA ATTCAACCGT 
GATGCTGCTG AGCTTTTGAT ATGGATGGAA GAGAAGTACA AGATTGCATC TGATGAATCC 
TATCGTGATC CAACAAATGT TTTACGCAAA CTGAAGTGGC ATGAGGCTGC TGAGAAGGAA 
ATGATGGCTA ATGAGAAGCA CTTCATAACA CTGATAAAGA AAGGAAATCA GCTGATTCAA 
GATGACCATT ATGCTGCAGC TTCTATCCAA AAGAAGATGT CAGAGCTAGA GAAGAAGTGG 
AAAGAATTGT ATGGGAAGAT GATAGAGCGA GGTGACAAGT TAAGACAAGC TGGGCAGCAA 
GAACAGCTCA TGGAACTGCT GCAGGATGCC AAAAAGAAGA TAGAGAAAAT TGAGAAGGTT 
CTTCAGGAAG CAGAGACTGG CCATGATTTG CGCTCCAGCC GTGATCTACT CAAGCAGCAC 
AGGCAGCTGG AAAATGAGAC ACACGAGCTG GCAGAGAAAA TGAACTCTAT TGTGTCTCAT 
GCAATGAAAA TGGCCACCAA CCACTTTGAC TCCCAGAGAA TTTTGGATGA AACGCAAAAA 
TACCTAAAAA GGTTTGAGTC TTTGCAAGCA CCTCTTGATG AGAGGCGTAA GCTGCTGGAA 
GCTGCAGTGG ATCTTTATGA GTTCTACCAT TACCATGACA TGGAGCTGAA CTGGATTAAT 
GAGCGACTGC CTATTGCCAG TTCCACCAAC TGTGGGAAGT CCTTGGATGT GGCTCAAAGT 
TTGTTGCAAA AACACAAGGA ATTTCAGGCA GAAGTGAATG CCCACAAGCA GCAAGTCCAG 
CGGGTCCTGG ATAAAGGAAG GACAATGATT GTAAACCAGC ATCCATCAGC TCAGAAAATA 
AATGAGAAAT GCCAGGAACT TGTGACTGCC TGGCAGGGCC TGGAGAAAGC CTGTGAGGAA 
AGGACAAAGC AGCTGCAGCA CTCAGTTGGT TTCCAAGAGT TTCTAATGAA TACTTCAGAC 
CTGGAGGCTT GGATAGCAGA AAAGCGTCCA CTTGTGATGA GCAAGGACTA TGGTAAGGAT 
GAAGATGGAA CATTGAAACT CCTCCAGAAA CATAAGGTGC TGGAGCACGA GATTGCTGTT 
TACCAAGATC TGATTAAAGA ACTAAATGAA AGTGTCCAAA ATCTTCCTGC CATGGGTTCC 
ATCCAGTACG TTGAGGTTGA TGCACCACGT GAACAAGTTC ACTCAAGACT CCGTGAGTTG 
CAGGAACTAG CAGCAACAAG AGGGAAGAAG CTGGATGAGA CTCTTGTCTT GCATGAATTC 
CTACGAGAAT ATGAAGATCT GCAGGACTGG ATGGCTCAGC AGAAACAAGC AGCCAGCTCT 
GAAGACTATG GAAATAATTA TGAGCATGTC TTGCAACTTC GTGCCAAATA TGACACTTTC 
CGACACCAAT TGGAAGCAAC TGGGAAAAGA GTTATGACTT GCCAGCAGCT GGCAGAGAAC 
TTGCTAAACG CTGGTCATTC TGAGTCCCGT GAAATCCGGC AGAAGCAGAA AGACCTAAGA 
AACTCTTGGG AGGAGCTGTT AGAAATTACC AGATTACGCG GTGAGCGGCT GAAAGATGCT 
GAGGTGATTC ACAAGTGTTA CCAAGATCTG ACAGATGCTC TGGCCCACAT TGAGGAGAAG 
TCCAAAAGTA TTCCAGATGA TGTTGCGAAG GACATGAGAG GTGTGCAAAC CCAGCTCCGG 
AACCATGTGG CCCTGGAACA TGAGCTCCTT GGAAATGAGC AGCAGCTGCA GGATCTGATC 
CACTCTGCAG ATCATGTGCT GACTCGTTGC TCAAATGAGC AGGCGTCAGG CCTGAAAGCA 
AAGCAACAGG CAATAGTGAC TAACTGGAAG GCCCTGAAGT CTAAAGTGCA ACTGCGTAAG 
GAGCTTCTGG AACAAGCACA CAAGCTTTAC CAGTTTCAGG CACAAGTGTG GGACTATTTC 
TCATGGACAT CAGAAATGAT TAGGGAAATG AGAGCCAAGG AGACTATCCG GGACATATCT 
ACTAGCAGCC TGAGACTTAC CCAGCATCAA CAGCTACTGG CTGAGATTGA AGCACAAGAA 
GAGAAGTATA ATCAAATTGT ACAGCTTGGA CAGTCACTCT TGCAAGATGA GGAAATAAGA 
TCAAAAGAGA TTCGTCAGAA GCTACAAGCT CTGTTGGAAG AGAAGGACAA AGTATACAGT 
GAATGGCAAC AGAAGAAGAA GTGGCTGGAC AGAATACATC AAGAACAAAT GTTCTACAAG 
GATTGGAATC ACCTGGACAA GCTCCTGAAC TCGCAGGAGG TTTATTTGAA AAGCAGTGAC 
CTTGGAAGCT CTGTAGATGA AGTAGAGCAA CTGATTAGGA AACATGAGGC ATTTGAGAAG 
CTACTGGCAT CACAGGATGA GAAGATAACG TCTCTTCAAG AACAAGCAAA CAAGCTAGAA 
AAGGATGGTG GATTGGATGG GGTACAGATT CAGCACAAGC TGGACACCAT TCTTCAGAGG 
AAGAAGCAGA TCAAGAATCT CTCCCGGAGC AGGAGAGAAA AGCTGCAGAC CGCCCTTCTT 
CTGGCACTTT TCTATCAGAA CTTGGCAGAG GCAGAAGACT GGATCAGTGA GCGCATGCAG 
AAGCTAGAGG ATCCTTCCAT ACAAGATCCC AGCAATCTGC AGGACAAAAT GAAGCTGCTG 
CAGAAGCATC AGGTCTTTGA GGCAGAGATT CTAGCAAATG AAGAAATCAT CACAGATGTT 
AATAAGAAAG GAGAAGCCCT GGTTTCAATG GGCCACCCAA AATCGGGGGA GATCCGCCGC 
CAAGTCCGCA TGCTTCAGGA GCGCTGGGAG AAGTTGAAGA GAGCTGTTGC TGCCCGTGGA 
AAGATGCTGG AGGATAGTCG AGATTTCCTG GAATTTCTCC AGAAAGTGGA CCAGGTGGAA 
GCCTGGATCA GGGAAAAGGA AGTCATGATT AACATAGGTG ATGTGGGAAA TGACTATGAA 
CACTGTCTGC AGCTCATCAG AAAGCTGAAT GAGTTCCGTG GAGCAACATC AGGGGAGACA 
ACAGTGGATG ATGCTCACAT CAAGGCTATC AATGCCCTGG CCATGAAACT GGAGAGACAA 
AACAAGGAAG AGACAAAGAC AATATATCAG CGCCGGAAGC AGCTCAATGA GAAGTGGAAC 
AGTTTCCATG GTAACCTGAA TGCATACAGG AGGAAGTTAG AAGGAGCTCT GGAGATTCAT 
GCCTTGATCA GAGAAATCGA TGACATCACA GAAAGAATTA CTGAGAAGAG TGTCCTGATA 
CAAGCACTGG ATTATGGGAA AGATGTTGAA AGTGTGGAAA ACCTTATTCG AAGACATGAA 
GAGATGGAGA GAGAAATCAA TGTTATTAAG TCCAAAATAG AGCCATTGGA ATTGGAATCT 
TTCCGCCTTT CCACAAGGAA TCCATCCATA AATGACAAGC TGACTATGAA GCAACAGGAG 
ATGAAAAACA ACTGGCTACG ACTCCAGGGA CAGGCCAAAC AAAGGAAGGA GAAGCTGGCA 
GCCTCATATC AGTTGCAGAA GTTTAACTCT GAGATGAAGG AAATTACAGA TTGGATTCAA 
AACATCAGAG GCTTAATGGA AGCAGGGGGG CTTCCTAAAA GCCTAAATGA AGCAGAAAGC 
ATGATTGAGG AGCATCAGCA GAGAAAGGAG GAGATTGAAG CACGAGTGGA AAGATTCAAC 
TCTCTCAGTA ACTATGGTCA ACAACTTGCC AATTCTGGTC ACTACGCAAC CCCTGAGATT 
CATCAGTCTC TCTCCAGACT ACAGAAAGCC TGGACTGAAT TGATCCAAGC ATGGCAGGAG 
CAATATATAA AATTGTTTCA AGCACAAGAT TTACAGAAAT TCTATGGCTA TGTGGAACAG 
ACCGAGAGCT GGCTCAGCAG CAAAGAGGCT TTCCTAGCCA ATGAAGACTT AGGGGACTCA 
GTGTCTAGTG TGGAGAGCCT GCAACGGAAA CACATGCAAT TTGAAAAATC CCTGGAAGCT 
CAGATGGAGA AAATTGATGA GATGGCTTCA TTTGCCCAGC AGCTAATTCA GAACAAACAT 
TATGACTCGG ACAACATCAC CAACAGATGC CAGGCCGTTC TGAGGAGAAA AGAGAAATTA 
CTGGAGAATG CTGCTGCTCG CAGACATCTA CTAGAGGAAT CAAGGCTTCT GCAGAAGTTG 
TTGAAGAATT CCTATGAGGT GGCTACCTGG ATCAATGAGA AGAACAGTAT TGCTCAGGAT 
GATAGCTGGA GGGATCCAAG CAATCTACAA ACCAAGCTGC AGAAACATCA AAGTTTCCAA 
GCAGAGATCA TGGCCAATAA AAATCGCCTG GACTCCATCA AATCTGAAGG AGAGAAGATG 
ATCCGTGAGA GACACTATGC CCCTGAGGCC ATTCAGTCTA GGCTACAAGA AATGGAAGAG 
CTATGGGAGG AACTGCTAGC AAGCTGCCAA GATAAACGAG CCAAGCTGCA GGATGCTTAC 
AAGGCCCTTC ATTTTCAGCG GAGTATAGAG GATACAGAAA AATGGTTGGA AGATGTGGAA 
AATGAGTTGA AAGCACCATA TAGCAGCGAT GATCTAGTGG TTCTGAACAG TCACCTGAAG 
AAACAAGAGG AACTGGAGCA AGATATTGCT AGCCATAGGG ACCGGTTGCA AGAGCTTGTG 
AACACAGCTC AGGAATTCCA GCAGGAAAAG CATTTCCTTG CCGATGAGCT AGAAGAGAGG 
GTCGATCAGC TGGTGCAAAG ATACAAAAGT CTACGTGATC CTCTGCAGGA GAGACGCGGG 
AGTTTGGAGG CAAGCAGACT TCAGTACGAG TTCTTCCGAG ATGTTGATGA GGAGTTGGCC 
TGGGTTCATG AGAAACTTCC CATGGCATCC TCCAGGGATT ACGGCCAATC CCTGGCAACT 
GTTCAGAGTC TACAGGAAAA GCACCAGAAT TTGGAGAATG AGATAAGCAG TAGGGATGCC 
TTGACCAAAG CAGTGCTCAG TACTGGGCAG AAACTGGTAA GGGGAGGCCA CTCAGCCTCT 
CGCAAGATTA TGGAGCAGAT GAAGGAGCTG GAGACATCTG TAGAAAACTT GAAAGCAGAG 
GCTCATGTGA GGAGACAGCG TCTCATGCAG TCATATGAGG CCCATCAGTT CCTGACTGAG 
CTACTGGATG TGGAGACCTG GCTGGCAGAG AGGAGCTTCA TTCTGGAGAC TTCTGATTAT 
GGGAAGAATG AAGAATCCAC TCAAGCTTTA CTACGTAAAC TTGAAGCCAC CAAGCTGGAC 
ATGGAGGGTT TTAGGCCCCG GATTGAGAAA GTACAGGAGA CAGGTGCCAG CTTAATAAAC 
AAAGACAACC CAGAGAGTCC AGCCATACTT TCAAAGCTCC AGAGGATCCT AGCAGACTAT 
CAGTCTCTCC TACAAAAGGC TGAGACCCAG AGAAAACGCC TGCAAGAACA ATTCCAGCTG 
TATCAGTTTG AAAGAGAATT CCAGCTGGTT GACGCCTGGC TTTCCAGTAA ACAGTCTATT 
GCAGAGTCCG ATGACTATGG GCAGGACTTA GATGGTGTTG AGGTGCTGGA GAAAAAGTTT 
AAAGATTTTG TAAATGAGAT TAAACCTCTG GGACATTCCA AAGTTCTCTC CTTAAATGAA 
TTAGCATCTA AACTAGAGAA GGATGGCCAC AGCAAGACAG ATATTATCCA GAAAAGGACA 
AAACAAATTA ACGAGATGTG GGAGAAACTG TGCCATGCCA TCCAGACAAG GACAGAGAAT 
CTGAGAGCAG CCCAGCAAGT TCACCAGTAT GACCATGATG TGGATGAAGT AAAGGGCTGG 
ATGCAGGAAA AGGAGGCTGT GGTGGACATA GATGATTATG GATATGATCT TCCAGGTGTA 
CAAACTCTTC TGAGCCAGCT TGAAGGAGTG GAGAGAGATC TAGGAGCCAT TATGAAAGAG 
CTGGAACGGA TTCGAGGAGA AGCCTGGCAC CTCAGCCGCA CATACCCACA GCTCAAGGAA 
AACATTATGG AGAGACTCAC AGATGTGGAT GAGTGCTGGA GAAACCTGGA TAAGAAGTTT 
CTGGAGAGAA AGGCGAGACT GAGCCAGGCA GAACAAGTCC AGCTCTACTT CAATGACTGC 
AGGGAGCTAA TGGCATGGGC CAATGAGATG CACGCACTAG TGATATCTGA GGAACTAGCA 
AATGATGTCC TGGGAGCTGA ACTGCTTATC AAACGTCATG ATGAATACAA GCGTGAGATA 
GAGAAGCAGT GGCTCAAATA TGAAGAAATA CAGCGGGCAG GAGATGACCT GACAAGAAAT 
GGACATTTCA TGTCTGTGGA GATTGAAGAA AAACTGCTAG AGCTGTCAGA GCTGATGAAG 
AAAGTAAAGG AGAGCTGGAA CGTGAGGAAA GAGCTGTATG AAGAAAACTG GGAGATTCAA 
CTGCTCCGCA GAGAACTAGA ACAAGCAGAA GCATGGCTGG CTGCCAAGGA GAGCTTTCTT 
TCAGACCCTA GCTATGGGGA TTCTGTGTCT GAAGTAGAGG AACTACTGAA GAAACATCAT 
GACTTTGAGA ACATGCTTGC AGCTCAGGAG GAGAAATTTG CTCAGCTCAG CAGGAAAACT 
AAGAGGGAGA TGAATCTTCT GAAACAGACA GATGCAGAAG AAAGTGAGCA GAAGGATAAA 
GGCAAAATTG TACGGGTTCC TTCTCTGAAA AGAAAAACAT CTGACAAAAG GAATCCACCA 
CCAAAAGTGA TAGAGATAAG GAATACAAAG CCACTGGAAT CTGTGCAACC ACCACTCCTG 
TCCTGGACTC CACTTGAAAC AATTTTCTCT CCAGTTGAAA AGCCTGCAGT GGCATTCCAA 
CAATTTGCTT CTGGGAAAGT CCCTGAGGTG CTGAGCAGCA AAAAACCAGA AATGAAACCT 
GAGAGGAGAC TCAGTGAGAC TATCACTCCA CCTACACCAA AGACAGTAGA CCCACAAGCT 
ACATCTTTGA CACATCACAG TTCTTTAACC TCTCCTTTAT CTCCTACTCC CATAAGCCTG 
CAACGCAGCA GCAATTTCTC AGAATCATAT GCCACAGGCC TTTTATCACC TCAAGAAAAA 
AGTGCTGAAA GCTCCTCCTT CTCCAACCTG CAAACAAAAT TAGCAGCAAC ATCACCCAAG 
CCTGACCAGA AGTCATTTGA TAAATCACCA GATTTACTGC TGTCTAGGTC CTCACGGGAG 
ACATCGGAGA CTGCACCCTT GGTTTCTGCA AGTCTCCAGC ATATGGAGGG GTTCCTAGAA 
AAGAGAGACC AGCTTCTTCC AGGACGACAA CAGCCAGGCT CAAGGTCTTG GAAATCCTTT 
TATGTAAAAC TTGATGGACT ACAGCTTGAT TTCTACAATG ATGAACAAGA AGCATCTAAG 
AATATATCCA CACTCCTGTC CCTCAGCATT GTTGGTGCCA AATGTGAGAG GCTGACAAAC 
TACATCAGGA AAGAGAACAC CTTCATGTTA CGACTGAGAG ACGGGGCAGA GTATTTCTTT 
GCAGCACCTT CTCCGATGCT GATGGAGAAC TGGTTGCAGT CACTGCAGAA TAATATAGGA 
CATAGAAACA GGCCAGATTC TACAGTGATG GTGAGGCCTT TCACTCCAAG CACTGAGCCC 
TCTCAGACAG ATCTGTGGGA CAGAGACTCT GCTGAAAGAC TAACCAGCAA AAGTACACTC 
CTAAGGAGAA CACCATCTTT CAGAATCAAA CCAGAGAAGG AATCTGCAGC ATTTTTCAGA 
AATTTAAAGG AAGATGGGAC TGCAGTGACC CAAGCCTCCA CCACCACTCT GAACCTAGAA 
CAAGGTGGTA ATAACACAGA ACTGCCTCCA TCTCTACTAG AAGCAGGAAG AGAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPTBN5 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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