sptbn5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sptbn5
Entrez Gene ID	569585
Full Name	spectrin, beta, non-erythrocytic 5
Synonyms	si:dkey-28g23.4
General protein information	Preferred Names spectrin, beta, non-erythrocytic 5 Names spectrin beta chain, non-erythrocytic 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021467432.1	XP_021323107.1	spectrin beta chain, non-erythrocytic 5 isoform X2
XM_017351710.1	XP_017207199.1	spectrin beta chain, non-erythrocytic 5
XM_021467431.1	XP_021323106.1	spectrin beta chain, non-erythrocytic 5 isoform X1
XM_021467434.1	XP_021323109.1	spectrin beta chain, non-erythrocytic 5 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sptbn5	XP_006500600.1
Gallus gallus (chicken)	SPTBN5	XP_421149.4
Drosophila melanogaster (fruit fly)	kst	NP_995988.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004440	XP_313728.5
Bos taurus (cattle)	SPTBN5	XP_003586608.2
Rattus norvegicus (Norway rat)	Sptbn5	XP_006234865.1
Danio rerio (zebrafish)	LOC569585	XP_698075.4
Caenorhabditis elegans (roundworm)	sma-1	NP_001256383.1
Xenopus tropicalis (tropical clawed frog)	sptbn5	XP_004917485.1
Homo sapiens (human)	SPTBN5	NP_057726.4
Pan troglodytes (chimpanzee)	SPTBN5	XP_001142930.3

Gene Ontology

The following sptbn5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sptbn5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa149735	XM_021467431.1 Latest version!	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa149736	XM_021467434.1 Latest version!	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa45808	XM_021467432.1 Latest version!	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa55643	XM_017351710.1 Latest version!	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sptbn5 ( XM_021467431.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323106.1 Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X1, mRNA.

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CloneID	ODa149735
Clone ID Related Accession (Same CDS sequence)	XM_021467431.1
Accession Version	XM_021467431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	spectrin beta chain, non-erythrocytic 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGG CTTTCAGTGG CTATTTTAGC AGTGAGTGGT TGCGATGGCA GAACATGAGA 
CATTCAATTA TTGAGCCGCA AAGGATTGTT CCAGAAGTGG GAAAGATTGT GGACCTTCTG 
AAAGAGCTGG ATGATATGAA GGGTCTGTAT GAGAGTATGG TGTCTGACTT GCTACGCTGG 
ATCAAGACCA AGGTGGTGGA GCTCAATGAC CGTTCCTTCC CCAACTCCCT GCGAGAAATG 
CAGCATCTGG TAGCTGCCTT TAAGAAATAC CGTACTATTG AGAAGCCTCC CAAATACCAG 
GAACGGGGGG CCATTGAGGC ACACCTCTTC GACTTACGAA CCAAGCTGAG GGCAAACAAC 
CAGAGGGACT ACATTCCTCC GGAAGGCAAG ACCTTGGGGG ATATTGAAAG GAACTGGACC 
TTGCTAGAGC GAGCAGAACA CCAGCGAGAA CGGGTTCTCC AGAGTGCACT GATGAGGCTG 
GAGCAGCTGG AGCAGTTGGC CCAGAAGTTT GGCCGTAAAG CGACGCTGAG GGAGAGCTAC 
CTGGAGGACA CTCTGCAACT GATTCAACAA CAAGACCTGG GAGGACTGCA GAGGCTGGAG 
GAGGCTGAGA TAGCAGCTCG CAGACTGGAG GCCCTGGGAG CTGATGTTTT GGCACATGAA 
CCACGTTTCC GTGCTTTAAG TGAAATGGCT GCTGTCATCG AGAGGGATAA CTACCACAGC 
AAAGTACAAG TGATTCGCAG AAATGTGGAC ATCATGAACC GCTGGAAGGA TTTGAAGCAG 
GTTCTGCAGG AGCAGAGAGC ACATGTTGGA GATGCGGCAA ATACATTTGC TGTCCTTAGA 
GACATTGAGT TAATATCAAT GGACCTCAAG GAATTACAGG CTCAGGCAGA TTCCTCGGAC 
ATGGGAAAAC AGCTGCCAGA TGTGGTAGCC CTACTGCAAA AGCAGGACTT GATTGACACT 
CAGATCATCT CACTTGGTGA AACTCTGAAT GCCATCAGTA GTAGCAGTGC CTTAAAAGTT 
AAACACAAAG GTGCCACAAA AGTCCAGAGA GAACTTAAAG ATCTAAATAG CCATTACAAC 
TCCCTACTGG GTCTCAGCAA GAACAGGAGA AAAGCTCTTG AGGGACAGCT GAAGCTCTTT 
GAGTTTTTCC ATGATTGTGA AGAAGTAGAA GCATGGATCT ATGAGAAATG GGTCCTCTTA 
CAGGCAGCAT CACTAGGGAG AGACCTCAGT CAAATTCAGC AGGCCATTCA GAACCACAAG 
GCTTTGGAGG CAGAGATTCA GTCACAGGAA TCTCTGTGCT CTGGGGTTGT ATTTAGAGGA 
CAAGAGCTTT GTAGAGGGAG ACACCCTAAT GAGAATGACA TCCAAAAGTG GATTCGGACT 
CTTCAGAAAC AGTGGCAGCA ACTTAAAGAA CAAGTCACCA TCCGAAAGAA CCGGCTGCAT 
GCTGCCAATG TCATCAAGCA GTATTTTGCA GACATTACTG AAGCCAGCTC TTGGCTTGAT 
GACCACAAAC CACTTCTCAT TGACGAGGAT TATGGAAAGG ATGAGGCCAG CTCTACTGCT 
CTGCTCCAGC GTCACCAACG AGTGGAGAAA GAAATGGAAG CATATTCCTC AGAAGTCAAA 
CGACTGGGTG AGCAGGCCAA GAGCGCTGCC CAACTTGCTC CTCTTACAAC TGAAACCCAG 
CAAACGAGAA TGGTCCACTA CAGTGATTCA TCAGATGGCG AAGATGAGAA GGAGAAAAAA 
GCTGACAAAG GAGGCAAAGC AGTCAGCAAG ATTTTACAAC AAGAACAAGC AATGATTAGG 
TTTAAATACA GAGGCCCTAA ACTAATCTGG GACAGAGGAG AAATATTGAC AATTATCAGC 
AGGGAAAAGG ATGATAAGTT GCTTCTTCGG GACAGCAGAG GAAATGAGCA ACTAGTCTCT 
TCCACATACA TCAGGGAGCT GCCAGTTTCT CAGGAAGCAC CACCTGAAAC TACAAACGGA 
GTAGCTGAGA GTCCAAAAAG AAAAGTGAGC CGCCCTCGCC GCACTCGATC CATGCGGCGT 
GGCACAGCTG AAATATCTAC CTCAACCTTA CCAGACCCAC ATTTCCAAAA AGACACAATA 
GAGAGCACAC AAAACAGTCT GGAAAAGGAT TTCAACCGCC TTTACAATTT AGCACAGTCA 
AAAAATAAGA TCCTGGAGGA CACTGCACGA CTACACAGAT TCTACAATGC TTGTGAGGAG 
TTTGAGTCCT GGATGGGAGA CAAGGAGAAT GTCCTCAACA CTTTCAGCTC CAATTCCAAC 
AACTTAGAGG TTGTACAAGC CAAATATGAG AATTTTGTAA CTGAACTAGC CAGTGGTAGA 
AAGCAGCTTG ATGATATTAC CAAGTTTGGA GATGAGCTAT TGAAACAACA GCACAGCAGG 
AAAAAAGACA TTCAGCAAAG ACTTGGAATT GTTACTCGCA GATGGGAACG TATTCAGAAA 
CTAAAGGATG AGATGGCTCA TGAGCTTCTG AGTTCTGCTG ATGTGAAGTC CTTCCTGCAG 
ACCTGCCAGG AAGTCCAAGC TCAGCTCCAG CACAGACTCG CCCAGCTTGA CACACCTGAT 
GTGGGGAGCT CACCATCAGC CTTACAAGCT GAGGAACACA GACAAGCCCA AGCTGAAAGG 
GACATAGAAG CCCTTGAAAG GAAAATAGAC TATCTTAAAA GTGTGGCCAA GATGAAAAAG 
GACTGTAGTC CTGCAGAAAG TGCTGCTATC ATGGAAGAAG TACATGCTCT GGAAGACTTG 
TTGCGGCAGT TAAAGGCTCA AGCCATTCAA AGACAACGAA TGCTTGAAGA AGCCAGACGC 
TTGCAACTCT TTCAAAAAGA GACCAGAGAC CTGTTGATGT GGGCTGAGGC AACACAGGAG 
CATCTTCTGG AAGAGGAGAC AGGATCTGAT GTAGCATCGG CTCAAGCCTT GTTGAAGGAG 
AACCTTGATC TCAAGCAGGA GATTGAACTG CAGAAAGCAA AATTAAAAGA TATGGAGAGG 
CTGGGTCAGT CTCTTGACGT GGCATCTGGA GAGAAAGGAG GTAAAGATGT TCAGCAGACA 
CTTACAAAGT TGGATCATGA GTGGAGCCAG CTGGACAAAC TGTGGTCTAG TCGCAACAGA 
AGGCTTGAAC AAGGACTGGA GTTTCAGAAA CTGAATATGG AAGCAGATCG TATTGAGGCC 
ACCATTTCTG GTCATGAAGC CAGACTTAAA GTCAGAGACT TAGGGGACTC TGTGGACAGT 
GTTCACAGTT TATTGAGCCG ACAAGAAGAG CTTGAGGCTT TGCTTGAGGC TCTAGAGCGG 
TCTATTATCC TCTTTCAAGA CAAGTGCCAG GAGCTCATCA GCAAGCGTAA CTTTGCTGCT 
AAAGAAATTC AACAGCGATC AAAGGTCATT CAAGATCATT TCAAGAGGCT GAAAGAAAGT 
TGTAAATTAA GGAGGCTTGA ATTACTTGCT TCGAAAAAGT ACCAGGAATT TTTAAGAGAT 
GCTGAGGAGA TGCTGTTGTG GATGGATGAA AAGTTTGAGA TCGCTGAGGA TGAATCGTAC 
AGAGATCCAA CCAACATCCT TCGTAAGCTT AAAAAACATG AGGCAGCAGA AAAAGAGATG 
CAGGCCAATG CAGTTCGCAT AGACAGACTA ATTGAGACTG GAGAGAAGAT GTTGGCAGAT 
GATCACTACA ACACCCAGAG CATTCAGAAA AAGACTCAAG AGGTACACAA AAGGTGGATT 
GAACTTCAGA GGAAGATGGC TGAAAGAGGG GACAAGCTGC GACAGGCTGG CCAACAGGAG 
CAACTCATGG AGCTGCTGCA GGATGCTAAA TTGAAGATTG AGGCAATTGA GAGAATGCTA 
CAAAATGCAA TCAGGGGTCA TGACCTCCGC TCCAGCAGAC AGCTACTAAA AGAGCACAAG 
CAGCTGGAAG AGGAAGCTCA AGAACTTGCT GAGAAAATCA ATTCTATTGT CACCCGTGCC 
AAAAACCTGG CCACCAACCA TTTTGACTCT CAGCGCATTC TCAAGGAGAC TGACACTTAC 
CTGAAACTAT TTAAGTCTCT AGAGAAACCT TTGGATCATC GCAGGAGCCA GTTAGAGGCA 
GATGTGGATC TATACAGCTT TTACCATGAT GTAGACCTAG AGCTAAACTG GATATCAGAG 
CATCAGCCTG TAGCTGATAC TACAAACTAT GAGAGATCAC TGGTTGGGGC AGTTAGCCTT 
CTTCAAAAAC ATAAGGACCT GCAGGCGGAG GTGAATGCCC ACAGCAAGTA CCTGCAGCGA 
GTACTGGACA GAGGACGAGC CATGGCCCGA TCCAATCAGT GGAATGGTCA GGAGGTGCAG 
CAGAGGTGTG AGCAATTGAT CGCAAAGTGG GAAGAGTTAG AGGAATCCTG CAAGAAACGC 
TCCGGAGAAC TTAACAGGGC TGTTACACGG GAACAGATTC TGCTTGACTG CACTGAGCTG 
GAAACCCTTG TGTCAGAAAC ATCAGCTTTA GTCAGCACTG ATTATGGCAA GACTGAATTA 
GCTACCCGCA GTCTAATAAA GCAACACGAG GGAGTGGAGG GGCAAATCGA GGTACTTGCT 
GCTCAAGTAG ATGAACTCAA GTCCAATGTC AAGCAGGCTG TACGTGTGTG GGGTTTAGAG 
GAGTTGAACA AGCCCTGCAA CCACATTAAA TCTAAGCTGA GTGAGCTTCA ACACTCAGCG 
ACAGTCAGAG AACAGAGGCT TCGAGAGACT CTACACCTAC ATGAATTTAA GCGTGAGACT 
AGTGAACTGG AGGAATGGAT TGCTCAGCAG ACACAGATTG CAGCCTCTAA TGATCTTGGG 
AGTGATTATG AAAATGCAAT GCAACTGCGT GGGAGATTTG AGGTCTTCTT GAAGCAGCTG 
GAGGTGGGAA AGGAGAGGAT GCACACCTGT GAACAGTTGG CTGACAAACT CATCAAAAGC 
AATCACCCTG AGAGTCGCTT CATCCGAGAA ACACAGAATC TGCTCAGAGA ATCGTGGGAC 
GAGCTGCAGG ATTTATCGAA AGATCGTAAA GAAAAGCTTC GTAAGTCTGA AAAATGTCAC 
AAATTCTACA AAGACCTGAC CGATGCACTG GGACAGATCA AGGAGAGGCA CAAAACCATT 
CCTGAAGACA TAGCCAAAGA TCTGAAAGGA GTGCAGTCAC AGCTACGCAA ACATGAAGCT 
CTTGTGCACG AGCTGGCTGG AACTGAGCAA CAGCTGCAGG AGCTGTTGGA TGGAACCGAT 
GCTATTTTGG GCATGTGCTC TCCTGAGCTG CGGGTGGGGT TGCAGGAACT ACAACAGGAG 
GTGGTGGAGA ACTGGGAGCG GCTCCGCATG CGGATGGAGC AGAGAGAAGA GGAGCTGCAA 
TCGGCTAAAG ACCGCTACAT GTTCCTCAAC ACGGCACAGG ACTACTCCCT GTGGTGCTCT 
CAGGTGCTGA GCTGGATGAA GGCAGAGGAG TCCATTCGAG ACGTTGCCAC CTGTGACCTG 
CAGTTGTTCC AGCACCAGCA GTTGTGGGCT GAGATTGTGG CCCGTGAGGA GACGTTTGCC 
CAGGCTGTGT CAATGGGTCA AGGCCTTCTG GAGAATGACA TACCTAATGC TAGAGAGATC 
CAGGAGAAAC TGAAAGCTCT GCAAGAAGAG AGAGAGAAGC TTTCCGATAA CTGGCAGCTC 
AGGCAGAAAT GGCTGGAGAA CACATATCTG GAGCAGGTGT TTTACAGAGA CACTGAGTAC 
ATGGAGAAGA TCACCAACTC TCAAGAGATC CAGTTGAAGA ACAGTGATTT AGGAACGACA 
GTGGATGATG TTGAAAGTTT GATTAAGCGC CATGAGGCCT TTGAAAAACT TCTTCATTCT 
CAGGAAGATA AGATGGTGGC TCTTCAGGAA TCAGCAGAGC GTTTAAAGAC AGAAGGTTTA 
ACACGAGAAA AAAACAGCAA TATTAAGAAC AAACTCAAAG CTCTTCAGGA AAGGAGGGAA 
CGGATTAAAG ATCTGTCTGG CAAACGAAGA GAGGATCTTG AGGTCTCAAA GCTTCACTGT 
ATTTTCAGCC GAGATGTGTC TGAGGCAGAG GAATGGATCA CAGATCGTAT GAACAAGATA 
CAAGATGACT CCAAAATGGA CATGAGCAAC CTTCAGACTA AAATGAAGAT GCTGCAGAAG 
CATCAGGTGT TTGAAGCTGA GATTCTGGCC CATGGAAATA TCATTGAATC TGTCCAGCAG 
GCTGGAAATG AGCTGGTGAC CATGCATCAC CCAAAATCAA AGGAGATACG CCAGACTGTT 
TCAGCGCTCA TCTCTCATTG GAAGGCTCTA AAGCAAGAGC TTGCGGTGCG TGGAAAGGTT 
CTAGAGGATA ACCGTGATTT TCTTGAGTTC CTGCAGAAAG TGGAACAGGT TGAAGTTTGG 
ATTAGGCAAA AGGAGGTGAT GATAAATGTT GGAGATGTCG GGGAGGATTA TGAACACGGT 
CAGCAGCTGC TGAAGAAGCT CAGTGAATTC AGAGGGTCAG GCTCTGGAGA TGTGACTGTT 
GACGATGCTC ACATCAAGAC AATCAACACT TTGGCCGCTC GACTTGAGAG ACAGAACAGT 
GATGAGCTGG TGACTGTGAA AAAGCGTAGG CAGCAGCTCA ATGAAAGATG GAGGAATTTC 
CATGGCAACC TGAGCAGCTA TAAGAGGAAG CTGGAGGCAG CGTTGGAGGT CCACGCATTG 
ATCCGAGAAC TAGAGGAGGT CCGGGACAGA GCTGGAGAGA AGATGCTGTT GCTCCAGGGT 
CAGGGTTGCG GGTTGGATGT TGAAAGTGTG GAGAACCTCA TTCGCAGACA TGAAGAGATG 
GAGAGGGAGG CCAGGGTCAT CCAGGAGAGA GGCGCTGCCC TGGAAAAGGA GACTAAAGAT 
CGGTTGAAGA GACACTGCGA TCTGTCTGAC AAACTTAGCA AGAAGCAGGA AGAGGTCAAA 
ATTGCTTTAG TCAAGCTGGA CAAGGAAATC AAACGAAGGA AGGAGCGATT GCAGGAGTCT 
CATCAGCTCC AGCTGTTTAA AGCGAATCAG CGTCTGCTTT TGGACTGGAC CCTCAAGCAA 
AGTGATGAGA TGGTGAAGAA AGGCCTGCCT AAGAATAAAA CCGAAGCTGA GAGTTTCATA 
TTAGAACATC ACGACTGGAA GGCTGAAATT GATGCTCGTG GCGATCGGAT AGATTCAGTT 
AAGAGTTTCG GACAGAATCT TGTGAAGTCT GGACACAGTG ATGCAGCAGA GATTAAAGAA 
GCCCTACGCA AGCTGGATGA TGCCAAGACA ATGCTTACCC GAGCTTGGGA GGACCGAAGA 
AAAACTCTGG ATCAAGCCCT GAAGCTCCAG ATATTCCTGG GCTATGCTGA CCAGACTGAG 
AGCTGGATGA GTAACAGGGA GGCTTTCTTG ATCAATGAAG ATCTAGGGGG TTCATTGTCG 
GAGGTGGAGG CTCTACAGAG GAAGCTGGCT CTGTTTGAGA ACTCTCTAGA AGCTCAGATG 
GAGCAGGTTT CAGAGGTTGA CAGATACGCT CAGCAGCTCA TACAGGCAAA CCACTACGAC 
TCTGACAACA TCAAGAAGAG GACAAAAGCT ATTCTGCTCA GGAAGGAAAG AATACTGGAA 
ATCAGCAAAT CCCGGCGAAA AGCTCTAGAA GAATCTCTTC AGTTGCAAAA GTTCCTGGAA 
GCAAGTTATG AGATTTCCTC ATGGCTGAAT GAGAAGAACT CAGTGGCTGT TGACGAGAGT 
TGGAAAGATC CTATAAATCT GCAGGCTAAG CTACTGAAAC ACCAGAGCTT TGAAGCAGAG 
ATTCTGGCCA ACCGCAACAG AGTGCAGGCA CACATTAAAG ATGGTGATAA CATGCTGGCT 
TTCAGTCATC CTGCTAAAAG CAAAATAAAA CCACGAATAA AGGATGTCGA TGACAACTGG 
GACCAGCTTT TAAGCAACTG CAAGCATAAG AAGACACGTC TACAGCAAGC GTATCAGGCC 
TTACAGTTCC AGCGTTCATT AGGTGACATT GAGGAGTGGC TTGGGTCGGT GGAGGTGGAA 
GTGGCGAATG ACGACTATGG AGGTGATCTG GCCTCTGTTA GCAGGCTGCT TAAGGCTCTA 
CAGGGTCTGG AGGAAATGGT AGACGCGCAC ATGGAGAAAG TCCAGGGGCT AGTGGACACT 
GCCAAAGACT TCAGCTCTCA GGGCAACTTC CTCGCAGATA ATATACAGCA GAGGGTTTGG 
GAGGTGGTCA ACAGGTATAA TGGCCTGGCT GAGCCGATGC AGACCCGCAG GGAGACTCTG 
GAGTCGTGGC AGCTGCTGTT CCAGTTCTAC AGGGACTTAG AGGATGAGCT GCTGTGGATT 
GAGGACAAGC TGCAGGCCAC GGCCACCAAA GACTGGGGCA CCTCCCTACA GAGCATACAA 
TCTATAGTCA AAAAACATCT GGTTGTGATG CAGGAAATTG AGAACCGAAC TCCTCTAATA 
ATGGCTGTAC AGGAGGCAGG ACAGAATTTG GTGCGAGGAC GACACTTTGC ATCACGTGAG 
ATCAGTGAAA AACTTTCAGG GTTAAAGAAG AACTTTGACG CCCTGAAGAA GGAGTCAGCG 
AATAAAGATC GCCTGCTTCA ACAAGCACTG AAAATCCAGA GCTTTCTCTC AGAGGTTTCC 
CAGTTGGAGC AGTGGATGGA GGAACAGAAG CCTGTTTTAG AGTCCCGCGA CTATGGAAAG 
AATGAGGAGG CCACTGAAGC TTTTCTCAGG AAGCTGGACA CAGTTGACCT GGAGTTGGAA 
AGCCAGCGTG GAAAAGTAGA GGCTCTCCTG AAAACCGGCA CAGATTTAGA GAAGTCTCAG 
CATCCCAACA GCCATTTAGT GAGTAAGAGC CTTGAAGATA TGCAGGGTGA GTTTGAGACA 
CTACTGCAAC TGTCTGGTGA ACGTCGCACA GAGCTGGAGA ATCAGTACAA TTTCTACATA 
TTTGAGAGAG ATGCCAGAGA CCTACAGACC TGGCTCCTTT CTCGGAAGAC CATAGCAGAA 
TCTGATGATT ATGGAAAAGA TCTGGAAGGA GTGGAAGCAC TGCAAAAGAA GTTTGAGGAT 
TTTTCAGAAG AGGTTGGGAC TCTGGGCCAG AGTAAGCTGA GCACTGTTCA AAAGCTAAAG 
CAAAGTGTGC AGTCAGCAGA TGCACAACAA AAAGAGAAAG ATCTACTCAA GCTCTGGGAG 
GATTTAAACA AGGCTATGAA AGCAAGAGCA GAGAACCTGC ATTCAGCTCG AGAAGTTCAC 
CAGTTTGAAC ATGACCTGGA TGAGCTTCGG ATCTGGATGA ATGAGAAGGA AGTTGCTCTG 
GACTCTGAGG AGCTGAAGAA TGACCTGCTT AGTGTCCAAG CTCTCATCCG TCAACATGAA 
GGACTGGAGA GAGACCTTGC AGTGATAGAA GAAGATGTGT CTCGTCGAAA AGAGGAGGGA 
AAAGCTTTGG TCCGCAGGTC ACCTCGAGTA AGGGACAGTT TGAGCAAGAG ACTCCAGGAA 
GTGGAGGAGA TCTGGAGAAA CCTGCTGGAG AAAGCCAACC AGCGCAGACA AAGACTCCAA 
CAGGCAGAGG CCGTTCAAAG ATATCTGATT GAATGGAGAG AGATGATGGG CTGGCTGAAA 
GAGACACTGT CTCTGATGAC AGGGGAGGGA TTCGGAGGCA AGGTTAAAGA CCTGGAACAG 
ATGATTAAAA AACATGATGA GTACCGCACA CAGATTGACA GACAACTGGA CAAATCGGAA 
ATGGTTAAAA ATGATGGGAG ACGTCTGATG GAGGAGGGGA ACTCATGGAG TCATGAGCTT 
GAGGAGCGTC TAGCCGAGCT CAAGGATCTG GAGGTGCGGG TGCTGGAGGC CTGGACTGAA 
AGGAGAGCTC AGTATCAGGA AGACATAGAG CTCCAGCAAC TGCAGAGGGA ACTGGAGCAG 
GCCGAGCACT GGCTCAACAC TTATGAGAGC GCGCTCAGAG CAGAGGACTA TGGGGAATCA 
GTTTCAGATG TTCTGGATTT GATGAAAAAG CAGGAAGATC TGGAGGCCAT GATTCAAGCA 
CAGAGTGAGA GATTCAATGC CCTTCAAGCC AGAAGAACAC AGAGGGAAAA TAAGATGAAA 
GAAATTCAAA GAGGTGACAG TACAGACAGC AGCAGGGACA AGCCTGTTCG TGTAACATCC 
CTAAAGCGAA AGCCATCTGA GCGGCCAGCA TTGCCCCCTC GTCCCTTGTT GACCAGTCCA 
GTCCTAAAGA ACAGCGATTC ACCCACACCT TCCCGAGTCC CTACCCCCAG GTCTGCCTTG 
TGGGAAAGCA GAAGTGATAA AACTGATTCT CCTGTGGTGT CACGAGTGGC AGCAGGACTC 
AGAAGAAAGA GCAGCAGTGA CAGTGATAGT GTAACATCAC CTGACAAACC ATCCACTTCT 
GGTCAGGATA GCCCTGACTC CTCCTCCACT GCGGTTTACA GACCTCATTA TCCACACAAT 
GCTTCTAAGG TTAAACATAC CACATCTTCA CAAGATGAAG TCGATACTCT AAGAGAGACT 
GAATCCTTTT TGAAAACCAT TGATAAACCA AAAATCACTC CTAGGCGTCA GAAACCATCA 
ACTCCTGATA AAGAGTTGTG GGGTCCTCCT ACATCCAAAC CACCTGAGCC ACCCATTAAA 
CCTTCACAAT CATTGTCCAG ATCTCCTCAG TCACTGTCCA GATCTCCTGA ACCACTGTCC 
AGATCTCCAG AGCCATTGCC TAGATCTCCT GAGCCACTGT CCAGATCTTC CCAGTCACAG 
CCCAGATCTC CTGAACCACT ATCCAAATCG CCTGAACCAC TATCCAGATC TCCTGAGGCA 
CTGTCCAGAT CTCCCGAGCC ACTGCCCAGA TCTCCTCAGT CACTGTCTAG ATCTCCTGAG 
CCACTGTCCA GATCTCCTGA GTCACCGTAT ATTCCTCCAC TCACAAAACC TCCATCCACA 
AACCCTCCTG ATCCACCATC CAAGGCCACA GAATTGGTGG ACAACCAAGA CTCAAAAGAT 
AGGAACCTTC CTCAATCCAC TCCACCATCA GTCCCTCAGT CATCACCTCA ATCTTTCGTT 
TCCAACCCAG AACCACAAGC GTCCTCTTCT GTGCAATCCT CAATAGTCTT TCCAGCTGCT 
TCCACCATGA TCAGAGCAGA AGATGCCAAA ATGGAGGGTA CGTTGGAGAT TAAACTGAAG 
CAAGGAGGAA ATACAGGCTT GGATCACTGG GAAACGGTCT ATGCACTACT GGAAGAGGAG 
ACACTCAATC TGTTTAAAGA CCAAGATGCC GCCACTGAGA ACTGTACACG CTGGCCACCG 
ATCAATTTGG CTGGAGCTAT TTGTAAAGAC AACCCTTTTT ACAGGAGAAA GGAGCACACG 
TTCAAACTCA TGCTGAGTGA TGGCAGTCAC TATCTGTTTG CTGCATCATC TCAAGGAGAA 
CAGCAGAAGT GGCTGAAGGA GCTCCAGGAA TGTATTAATC CAACTGTGTC TTCAGACAAT 
CCTGGAATGA AGAGAGAATC AAGCAGTGAA AGCGTAGAGA AGAGCGAGTC TGTGCCAGTC 
GACACATCTG ACACACAAGA CCAGGAGAAG GAAGACTCCA CCGAGAGCTG TGAGACAGAG 
AGAGAACCAC CACCAAAACC TCCACACACG TACTACAACA AACACAAATA CCCTGACGGA 
GGAGAGAGCC AAGACTCAGT GAGTGTGTCT CAAAATATAA AAGGGCTACA GCCTCCCACA 
TTCGCTCCTC CTCCTCCTCC AACACAAAAC CAGACAGAGA ATGAAGCCAA GGCCAAAAAC 
AGGAGCGGAT TTATGAAGTT TTTCAGAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323106.1
CDS	536..12268
Translation	MTMAFSGYFSSEWLRWQNMRHSIIEPQRIVPEVGKIVDLLKELDDMKGLYESMVSDLLRWIKTKVVELNDRSFPNSLREMQHLVAAFKKYRTIEKPPKYQERGAIEAHLFDLRTKLRANNQRDYIPPEGKTLGDIERNWTLLERAEHQRERVLQSALMRLEQLEQLAQKFGRKATLRESYLEDTLQLIQQQDLGGLQRLEEAEIAARRLEALGADVLAHEPRFRALSEMAAVIERDNYHSKVQVIRRNVDIMNRWKDLKQVLQEQRAHVGDAANTFAVLRDIELISMDLKELQAQADSSDMGKQLPDVVALLQKQDLIDTQIISLGETLNAISSSSALKVKHKGATKVQRELKDLNSHYNSLLGLSKNRRKALEGQLKLFEFFHDCEEVEAWIYEKWVLLQAASLGRDLSQIQQAIQNHKALEAEIQSQESLCSGVVFRGQELCRGRHPNENDIQKWIRTLQKQWQQLKEQVTIRKNRLHAANVIKQYFADITEASSWLDDHKPLLIDEDYGKDEASSTALLQRHQRVEKEMEAYSSEVKRLGEQAKSAAQLAPLTTETQQTRMVHYSDSSDGEDEKEKKADKGGKAVSKILQQEQAMIRFKYRGPKLIWDRGEILTIISREKDDKLLLRDSRGNEQLVSSTYIRELPVSQEAPPETTNGVAESPKRKVSRPRRTRSMRRGTAEISTSTLPDPHFQKDTIESTQNSLEKDFNRLYNLAQSKNKILEDTARLHRFYNACEEFESWMGDKENVLNTFSSNSNNLEVVQAKYENFVTELASGRKQLDDITKFGDELLKQQHSRKKDIQQRLGIVTRRWERIQKLKDEMAHELLSSADVKSFLQTCQEVQAQLQHRLAQLDTPDVGSSPSALQAEEHRQAQAERDIEALERKIDYLKSVAKMKKDCSPAESAAIMEEVHALEDLLRQLKAQAIQRQRMLEEARRLQLFQKETRDLLMWAEATQEHLLEEETGSDVASAQALLKENLDLKQEIELQKAKLKDMERLGQSLDVASGEKGGKDVQQTLTKLDHEWSQLDKLWSSRNRRLEQGLEFQKLNMEADRIEATISGHEARLKVRDLGDSVDSVHSLLSRQEELEALLEALERSIILFQDKCQELISKRNFAAKEIQQRSKVIQDHFKRLKESCKLRRLELLASKKYQEFLRDAEEMLLWMDEKFEIAEDESYRDPTNILRKLKKHEAAEKEMQANAVRIDRLIETGEKMLADDHYNTQSIQKKTQEVHKRWIELQRKMAERGDKLRQAGQQEQLMELLQDAKLKIEAIERMLQNAIRGHDLRSSRQLLKEHKQLEEEAQELAEKINSIVTRAKNLATNHFDSQRILKETDTYLKLFKSLEKPLDHRRSQLEADVDLYSFYHDVDLELNWISEHQPVADTTNYERSLVGAVSLLQKHKDLQAEVNAHSKYLQRVLDRGRAMARSNQWNGQEVQQRCEQLIAKWEELEESCKKRSGELNRAVTREQILLDCTELETLVSETSALVSTDYGKTELATRSLIKQHEGVEGQIEVLAAQVDELKSNVKQAVRVWGLEELNKPCNHIKSKLSELQHSATVREQRLRETLHLHEFKRETSELEEWIAQQTQIAASNDLGSDYENAMQLRGRFEVFLKQLEVGKERMHTCEQLADKLIKSNHPESRFIRETQNLLRESWDELQDLSKDRKEKLRKSEKCHKFYKDLTDALGQIKERHKTIPEDIAKDLKGVQSQLRKHEALVHELAGTEQQLQELLDGTDAILGMCSPELRVGLQELQQEVVENWERLRMRMEQREEELQSAKDRYMFLNTAQDYSLWCSQVLSWMKAEESIRDVATCDLQLFQHQQLWAEIVAREETFAQAVSMGQGLLENDIPNAREIQEKLKALQEEREKLSDNWQLRQKWLENTYLEQVFYRDTEYMEKITNSQEIQLKNSDLGTTVDDVESLIKRHEAFEKLLHSQEDKMVALQESAERLKTEGLTREKNSNIKNKLKALQERRERIKDLSGKRREDLEVSKLHCIFSRDVSEAEEWITDRMNKIQDDSKMDMSNLQTKMKMLQKHQVFEAEILAHGNIIESVQQAGNELVTMHHPKSKEIRQTVSALISHWKALKQELAVRGKVLEDNRDFLEFLQKVEQVEVWIRQKEVMINVGDVGEDYEHGQQLLKKLSEFRGSGSGDVTVDDAHIKTINTLAARLERQNSDELVTVKKRRQQLNERWRNFHGNLSSYKRKLEAALEVHALIRELEEVRDRAGEKMLLLQGQGCGLDVESVENLIRRHEEMEREARVIQERGAALEKETKDRLKRHCDLSDKLSKKQEEVKIALVKLDKEIKRRKERLQESHQLQLFKANQRLLLDWTLKQSDEMVKKGLPKNKTEAESFILEHHDWKAEIDARGDRIDSVKSFGQNLVKSGHSDAAEIKEALRKLDDAKTMLTRAWEDRRKTLDQALKLQIFLGYADQTESWMSNREAFLINEDLGGSLSEVEALQRKLALFENSLEAQMEQVSEVDRYAQQLIQANHYDSDNIKKRTKAILLRKERILEISKSRRKALEESLQLQKFLEASYEISSWLNEKNSVAVDESWKDPINLQAKLLKHQSFEAEILANRNRVQAHIKDGDNMLAFSHPAKSKIKPRIKDVDDNWDQLLSNCKHKKTRLQQAYQALQFQRSLGDIEEWLGSVEVEVANDDYGGDLASVSRLLKALQGLEEMVDAHMEKVQGLVDTAKDFSSQGNFLADNIQQRVWEVVNRYNGLAEPMQTRRETLESWQLLFQFYRDLEDELLWIEDKLQATATKDWGTSLQSIQSIVKKHLVVMQEIENRTPLIMAVQEAGQNLVRGRHFASREISEKLSGLKKNFDALKKESANKDRLLQQALKIQSFLSEVSQLEQWMEEQKPVLESRDYGKNEEATEAFLRKLDTVDLELESQRGKVEALLKTGTDLEKSQHPNSHLVSKSLEDMQGEFETLLQLSGERRTELENQYNFYIFERDARDLQTWLLSRKTIAESDDYGKDLEGVEALQKKFEDFSEEVGTLGQSKLSTVQKLKQSVQSADAQQKEKDLLKLWEDLNKAMKARAENLHSAREVHQFEHDLDELRIWMNEKEVALDSEELKNDLLSVQALIRQHEGLERDLAVIEEDVSRRKEEGKALVRRSPRVRDSLSKRLQEVEEIWRNLLEKANQRRQRLQQAEAVQRYLIEWREMMGWLKETLSLMTGEGFGGKVKDLEQMIKKHDEYRTQIDRQLDKSEMVKNDGRRLMEEGNSWSHELEERLAELKDLEVRVLEAWTERRAQYQEDIELQQLQRELEQAEHWLNTYESALRAEDYGESVSDVLDLMKKQEDLEAMIQAQSERFNALQARRTQRENKMKEIQRGDSTDSSRDKPVRVTSLKRKPSERPALPPRPLLTSPVLKNSDSPTPSRVPTPRSALWESRSDKTDSPVVSRVAAGLRRKSSSDSDSVTSPDKPSTSGQDSPDSSSTAVYRPHYPHNASKVKHTTSSQDEVDTLRETESFLKTIDKPKITPRRQKPSTPDKELWGPPTSKPPEPPIKPSQSLSRSPQSLSRSPEPLSRSPEPLPRSPEPLSRSSQSQPRSPEPLSKSPEPLSRSPEALSRSPEPLPRSPQSLSRSPEPLSRSPESPYIPPLTKPPSTNPPDPPSKATELVDNQDSKDRNLPQSTPPSVPQSSPQSFVSNPEPQASSSVQSSIVFPAASTMIRAEDAKMEGTLEIKLKQGGNTGLDHWETVYALLEEETLNLFKDQDAATENCTRWPPINLAGAICKDNPFYRRKEHTFKLMLSDGSHYLFAASSQGEQQKWLKELQECINPTVSSDNPGMKRESSSESVEKSESVPVDTSDTQDQEKEDSTESCETEREPPPKPPHTYYNKHKYPDGGESQDSVSVSQNIKGLQPPTFAPPPPPTQNQTENEAKAKNRSGFMKFFRK

Target ORF information:

RefSeq Version	XM_021467431.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467431.1

ORF Insert Sequence:

ATGACAATGG CTTTCAGTGG CTATTTTAGC AGTGAGTGGT TGCGATGGCA GAACATGAGA 
CATTCAATTA TTGAGCCGCA AAGGATTGTT CCAGAAGTGG GAAAGATTGT GGACCTTCTG 
AAAGAGCTGG ATGATATGAA GGGTCTGTAT GAGAGTATGG TGTCTGACTT GCTACGCTGG 
ATCAAGACCA AGGTGGTGGA GCTCAATGAC CGTTCCTTCC CCAACTCCCT GCGAGAAATG 
CAGCATCTGG TAGCTGCCTT TAAGAAATAC CGTACTATTG AGAAGCCTCC CAAATACCAG 
GAACGGGGGG CCATTGAGGC ACACCTCTTC GACTTACGAA CCAAGCTGAG GGCAAACAAC 
CAGAGGGACT ACATTCCTCC GGAAGGCAAG ACCTTGGGGG ATATTGAAAG GAACTGGACC 
TTGCTAGAGC GAGCAGAACA CCAGCGAGAA CGGGTTCTCC AGAGTGCACT GATGAGGCTG 
GAGCAGCTGG AGCAGTTGGC CCAGAAGTTT GGCCGTAAAG CGACGCTGAG GGAGAGCTAC 
CTGGAGGACA CTCTGCAACT GATTCAACAA CAAGACCTGG GAGGACTGCA GAGGCTGGAG 
GAGGCTGAGA TAGCAGCTCG CAGACTGGAG GCCCTGGGAG CTGATGTTTT GGCACATGAA 
CCACGTTTCC GTGCTTTAAG TGAAATGGCT GCTGTCATCG AGAGGGATAA CTACCACAGC 
AAAGTACAAG TGATTCGCAG AAATGTGGAC ATCATGAACC GCTGGAAGGA TTTGAAGCAG 
GTTCTGCAGG AGCAGAGAGC ACATGTTGGA GATGCGGCAA ATACATTTGC TGTCCTTAGA 
GACATTGAGT TAATATCAAT GGACCTCAAG GAATTACAGG CTCAGGCAGA TTCCTCGGAC 
ATGGGAAAAC AGCTGCCAGA TGTGGTAGCC CTACTGCAAA AGCAGGACTT GATTGACACT 
CAGATCATCT CACTTGGTGA AACTCTGAAT GCCATCAGTA GTAGCAGTGC CTTAAAAGTT 
AAACACAAAG GTGCCACAAA AGTCCAGAGA GAACTTAAAG ATCTAAATAG CCATTACAAC 
TCCCTACTGG GTCTCAGCAA GAACAGGAGA AAAGCTCTTG AGGGACAGCT GAAGCTCTTT 
GAGTTTTTCC ATGATTGTGA AGAAGTAGAA GCATGGATCT ATGAGAAATG GGTCCTCTTA 
CAGGCAGCAT CACTAGGGAG AGACCTCAGT CAAATTCAGC AGGCCATTCA GAACCACAAG 
GCTTTGGAGG CAGAGATTCA GTCACAGGAA TCTCTGTGCT CTGGGGTTGT ATTTAGAGGA 
CAAGAGCTTT GTAGAGGGAG ACACCCTAAT GAGAATGACA TCCAAAAGTG GATTCGGACT 
CTTCAGAAAC AGTGGCAGCA ACTTAAAGAA CAAGTCACCA TCCGAAAGAA CCGGCTGCAT 
GCTGCCAATG TCATCAAGCA GTATTTTGCA GACATTACTG AAGCCAGCTC TTGGCTTGAT 
GACCACAAAC CACTTCTCAT TGACGAGGAT TATGGAAAGG ATGAGGCCAG CTCTACTGCT 
CTGCTCCAGC GTCACCAACG AGTGGAGAAA GAAATGGAAG CATATTCCTC AGAAGTCAAA 
CGACTGGGTG AGCAGGCCAA GAGCGCTGCC CAACTTGCTC CTCTTACAAC TGAAACCCAG 
CAAACGAGAA TGGTCCACTA CAGTGATTCA TCAGATGGCG AAGATGAGAA GGAGAAAAAA 
GCTGACAAAG GAGGCAAAGC AGTCAGCAAG ATTTTACAAC AAGAACAAGC AATGATTAGG 
TTTAAATACA GAGGCCCTAA ACTAATCTGG GACAGAGGAG AAATATTGAC AATTATCAGC 
AGGGAAAAGG ATGATAAGTT GCTTCTTCGG GACAGCAGAG GAAATGAGCA ACTAGTCTCT 
TCCACATACA TCAGGGAGCT GCCAGTTTCT CAGGAAGCAC CACCTGAAAC TACAAACGGA 
GTAGCTGAGA GTCCAAAAAG AAAAGTGAGC CGCCCTCGCC GCACTCGATC CATGCGGCGT 
GGCACAGCTG AAATATCTAC CTCAACCTTA CCAGACCCAC ATTTCCAAAA AGACACAATA 
GAGAGCACAC AAAACAGTCT GGAAAAGGAT TTCAACCGCC TTTACAATTT AGCACAGTCA 
AAAAATAAGA TCCTGGAGGA CACTGCACGA CTACACAGAT TCTACAATGC TTGTGAGGAG 
TTTGAGTCCT GGATGGGAGA CAAGGAGAAT GTCCTCAACA CTTTCAGCTC CAATTCCAAC 
AACTTAGAGG TTGTACAAGC CAAATATGAG AATTTTGTAA CTGAACTAGC CAGTGGTAGA 
AAGCAGCTTG ATGATATTAC CAAGTTTGGA GATGAGCTAT TGAAACAACA GCACAGCAGG 
AAAAAAGACA TTCAGCAAAG ACTTGGAATT GTTACTCGCA GATGGGAACG TATTCAGAAA 
CTAAAGGATG AGATGGCTCA TGAGCTTCTG AGTTCTGCTG ATGTGAAGTC CTTCCTGCAG 
ACCTGCCAGG AAGTCCAAGC TCAGCTCCAG CACAGACTCG CCCAGCTTGA CACACCTGAT 
GTGGGGAGCT CACCATCAGC CTTACAAGCT GAGGAACACA GACAAGCCCA AGCTGAAAGG 
GACATAGAAG CCCTTGAAAG GAAAATAGAC TATCTTAAAA GTGTGGCCAA GATGAAAAAG 
GACTGTAGTC CTGCAGAAAG TGCTGCTATC ATGGAAGAAG TACATGCTCT GGAAGACTTG 
TTGCGGCAGT TAAAGGCTCA AGCCATTCAA AGACAACGAA TGCTTGAAGA AGCCAGACGC 
TTGCAACTCT TTCAAAAAGA GACCAGAGAC CTGTTGATGT GGGCTGAGGC AACACAGGAG 
CATCTTCTGG AAGAGGAGAC AGGATCTGAT GTAGCATCGG CTCAAGCCTT GTTGAAGGAG 
AACCTTGATC TCAAGCAGGA GATTGAACTG CAGAAAGCAA AATTAAAAGA TATGGAGAGG 
CTGGGTCAGT CTCTTGACGT GGCATCTGGA GAGAAAGGAG GTAAAGATGT TCAGCAGACA 
CTTACAAAGT TGGATCATGA GTGGAGCCAG CTGGACAAAC TGTGGTCTAG TCGCAACAGA 
AGGCTTGAAC AAGGACTGGA GTTTCAGAAA CTGAATATGG AAGCAGATCG TATTGAGGCC 
ACCATTTCTG GTCATGAAGC CAGACTTAAA GTCAGAGACT TAGGGGACTC TGTGGACAGT 
GTTCACAGTT TATTGAGCCG ACAAGAAGAG CTTGAGGCTT TGCTTGAGGC TCTAGAGCGG 
TCTATTATCC TCTTTCAAGA CAAGTGCCAG GAGCTCATCA GCAAGCGTAA CTTTGCTGCT 
AAAGAAATTC AACAGCGATC AAAGGTCATT CAAGATCATT TCAAGAGGCT GAAAGAAAGT 
TGTAAATTAA GGAGGCTTGA ATTACTTGCT TCGAAAAAGT ACCAGGAATT TTTAAGAGAT 
GCTGAGGAGA TGCTGTTGTG GATGGATGAA AAGTTTGAGA TCGCTGAGGA TGAATCGTAC 
AGAGATCCAA CCAACATCCT TCGTAAGCTT AAAAAACATG AGGCAGCAGA AAAAGAGATG 
CAGGCCAATG CAGTTCGCAT AGACAGACTA ATTGAGACTG GAGAGAAGAT GTTGGCAGAT 
GATCACTACA ACACCCAGAG CATTCAGAAA AAGACTCAAG AGGTACACAA AAGGTGGATT 
GAACTTCAGA GGAAGATGGC TGAAAGAGGG GACAAGCTGC GACAGGCTGG CCAACAGGAG 
CAACTCATGG AGCTGCTGCA GGATGCTAAA TTGAAGATTG AGGCAATTGA GAGAATGCTA 
CAAAATGCAA TCAGGGGTCA TGACCTCCGC TCCAGCAGAC AGCTACTAAA AGAGCACAAG 
CAGCTGGAAG AGGAAGCTCA AGAACTTGCT GAGAAAATCA ATTCTATTGT CACCCGTGCC 
AAAAACCTGG CCACCAACCA TTTTGACTCT CAGCGCATTC TCAAGGAGAC TGACACTTAC 
CTGAAACTAT TTAAGTCTCT AGAGAAACCT TTGGATCATC GCAGGAGCCA GTTAGAGGCA 
GATGTGGATC TATACAGCTT TTACCATGAT GTAGACCTAG AGCTAAACTG GATATCAGAG 
CATCAGCCTG TAGCTGATAC TACAAACTAT GAGAGATCAC TGGTTGGGGC AGTTAGCCTT 
CTTCAAAAAC ATAAGGACCT GCAGGCGGAG GTGAATGCCC ACAGCAAGTA CCTGCAGCGA 
GTACTGGACA GAGGACGAGC CATGGCCCGA TCCAATCAGT GGAATGGTCA GGAGGTGCAG 
CAGAGGTGTG AGCAATTGAT CGCAAAGTGG GAAGAGTTAG AGGAATCCTG CAAGAAACGC 
TCCGGAGAAC TTAACAGGGC TGTTACACGG GAACAGATTC TGCTTGACTG CACTGAGCTG 
GAAACCCTTG TGTCAGAAAC ATCAGCTTTA GTCAGCACTG ATTATGGCAA GACTGAATTA 
GCTACCCGCA GTCTAATAAA GCAACACGAG GGAGTGGAGG GGCAAATCGA GGTACTTGCT 
GCTCAAGTAG ATGAACTCAA GTCCAATGTC AAGCAGGCTG TACGTGTGTG GGGTTTAGAG 
GAGTTGAACA AGCCCTGCAA CCACATTAAA TCTAAGCTGA GTGAGCTTCA ACACTCAGCG 
ACAGTCAGAG AACAGAGGCT TCGAGAGACT CTACACCTAC ATGAATTTAA GCGTGAGACT 
AGTGAACTGG AGGAATGGAT TGCTCAGCAG ACACAGATTG CAGCCTCTAA TGATCTTGGG 
AGTGATTATG AAAATGCAAT GCAACTGCGT GGGAGATTTG AGGTCTTCTT GAAGCAGCTG 
GAGGTGGGAA AGGAGAGGAT GCACACCTGT GAACAGTTGG CTGACAAACT CATCAAAAGC 
AATCACCCTG AGAGTCGCTT CATCCGAGAA ACACAGAATC TGCTCAGAGA ATCGTGGGAC 
GAGCTGCAGG ATTTATCGAA AGATCGTAAA GAAAAGCTTC GTAAGTCTGA AAAATGTCAC 
AAATTCTACA AAGACCTGAC CGATGCACTG GGACAGATCA AGGAGAGGCA CAAAACCATT 
CCTGAAGACA TAGCCAAAGA TCTGAAAGGA GTGCAGTCAC AGCTACGCAA ACATGAAGCT 
CTTGTGCACG AGCTGGCTGG AACTGAGCAA CAGCTGCAGG AGCTGTTGGA TGGAACCGAT 
GCTATTTTGG GCATGTGCTC TCCTGAGCTG CGGGTGGGGT TGCAGGAACT ACAACAGGAG 
GTGGTGGAGA ACTGGGAGCG GCTCCGCATG CGGATGGAGC AGAGAGAAGA GGAGCTGCAA 
TCGGCTAAAG ACCGCTACAT GTTCCTCAAC ACGGCACAGG ACTACTCCCT GTGGTGCTCT 
CAGGTGCTGA GCTGGATGAA GGCAGAGGAG TCCATTCGAG ACGTTGCCAC CTGTGACCTG 
CAGTTGTTCC AGCACCAGCA GTTGTGGGCT GAGATTGTGG CCCGTGAGGA GACGTTTGCC 
CAGGCTGTGT CAATGGGTCA AGGCCTTCTG GAGAATGACA TACCTAATGC TAGAGAGATC 
CAGGAGAAAC TGAAAGCTCT GCAAGAAGAG AGAGAGAAGC TTTCCGATAA CTGGCAGCTC 
AGGCAGAAAT GGCTGGAGAA CACATATCTG GAGCAGGTGT TTTACAGAGA CACTGAGTAC 
ATGGAGAAGA TCACCAACTC TCAAGAGATC CAGTTGAAGA ACAGTGATTT AGGAACGACA 
GTGGATGATG TTGAAAGTTT GATTAAGCGC CATGAGGCCT TTGAAAAACT TCTTCATTCT 
CAGGAAGATA AGATGGTGGC TCTTCAGGAA TCAGCAGAGC GTTTAAAGAC AGAAGGTTTA 
ACACGAGAAA AAAACAGCAA TATTAAGAAC AAACTCAAAG CTCTTCAGGA AAGGAGGGAA 
CGGATTAAAG ATCTGTCTGG CAAACGAAGA GAGGATCTTG AGGTCTCAAA GCTTCACTGT 
ATTTTCAGCC GAGATGTGTC TGAGGCAGAG GAATGGATCA CAGATCGTAT GAACAAGATA 
CAAGATGACT CCAAAATGGA CATGAGCAAC CTTCAGACTA AAATGAAGAT GCTGCAGAAG 
CATCAGGTGT TTGAAGCTGA GATTCTGGCC CATGGAAATA TCATTGAATC TGTCCAGCAG 
GCTGGAAATG AGCTGGTGAC CATGCATCAC CCAAAATCAA AGGAGATACG CCAGACTGTT 
TCAGCGCTCA TCTCTCATTG GAAGGCTCTA AAGCAAGAGC TTGCGGTGCG TGGAAAGGTT 
CTAGAGGATA ACCGTGATTT TCTTGAGTTC CTGCAGAAAG TGGAACAGGT TGAAGTTTGG 
ATTAGGCAAA AGGAGGTGAT GATAAATGTT GGAGATGTCG GGGAGGATTA TGAACACGGT 
CAGCAGCTGC TGAAGAAGCT CAGTGAATTC AGAGGGTCAG GCTCTGGAGA TGTGACTGTT 
GACGATGCTC ACATCAAGAC AATCAACACT TTGGCCGCTC GACTTGAGAG ACAGAACAGT 
GATGAGCTGG TGACTGTGAA AAAGCGTAGG CAGCAGCTCA ATGAAAGATG GAGGAATTTC 
CATGGCAACC TGAGCAGCTA TAAGAGGAAG CTGGAGGCAG CGTTGGAGGT CCACGCATTG 
ATCCGAGAAC TAGAGGAGGT CCGGGACAGA GCTGGAGAGA AGATGCTGTT GCTCCAGGGT 
CAGGGTTGCG GGTTGGATGT TGAAAGTGTG GAGAACCTCA TTCGCAGACA TGAAGAGATG 
GAGAGGGAGG CCAGGGTCAT CCAGGAGAGA GGCGCTGCCC TGGAAAAGGA GACTAAAGAT 
CGGTTGAAGA GACACTGCGA TCTGTCTGAC AAACTTAGCA AGAAGCAGGA AGAGGTCAAA 
ATTGCTTTAG TCAAGCTGGA CAAGGAAATC AAACGAAGGA AGGAGCGATT GCAGGAGTCT 
CATCAGCTCC AGCTGTTTAA AGCGAATCAG CGTCTGCTTT TGGACTGGAC CCTCAAGCAA 
AGTGATGAGA TGGTGAAGAA AGGCCTGCCT AAGAATAAAA CCGAAGCTGA GAGTTTCATA 
TTAGAACATC ACGACTGGAA GGCTGAAATT GATGCTCGTG GCGATCGGAT AGATTCAGTT 
AAGAGTTTCG GACAGAATCT TGTGAAGTCT GGACACAGTG ATGCAGCAGA GATTAAAGAA 
GCCCTACGCA AGCTGGATGA TGCCAAGACA ATGCTTACCC GAGCTTGGGA GGACCGAAGA 
AAAACTCTGG ATCAAGCCCT GAAGCTCCAG ATATTCCTGG GCTATGCTGA CCAGACTGAG 
AGCTGGATGA GTAACAGGGA GGCTTTCTTG ATCAATGAAG ATCTAGGGGG TTCATTGTCG 
GAGGTGGAGG CTCTACAGAG GAAGCTGGCT CTGTTTGAGA ACTCTCTAGA AGCTCAGATG 
GAGCAGGTTT CAGAGGTTGA CAGATACGCT CAGCAGCTCA TACAGGCAAA CCACTACGAC 
TCTGACAACA TCAAGAAGAG GACAAAAGCT ATTCTGCTCA GGAAGGAAAG AATACTGGAA 
ATCAGCAAAT CCCGGCGAAA AGCTCTAGAA GAATCTCTTC AGTTGCAAAA GTTCCTGGAA 
GCAAGTTATG AGATTTCCTC ATGGCTGAAT GAGAAGAACT CAGTGGCTGT TGACGAGAGT 
TGGAAAGATC CTATAAATCT GCAGGCTAAG CTACTGAAAC ACCAGAGCTT TGAAGCAGAG 
ATTCTGGCCA ACCGCAACAG AGTGCAGGCA CACATTAAAG ATGGTGATAA CATGCTGGCT 
TTCAGTCATC CTGCTAAAAG CAAAATAAAA CCACGAATAA AGGATGTCGA TGACAACTGG 
GACCAGCTTT TAAGCAACTG CAAGCATAAG AAGACACGTC TACAGCAAGC GTATCAGGCC 
TTACAGTTCC AGCGTTCATT AGGTGACATT GAGGAGTGGC TTGGGTCGGT GGAGGTGGAA 
GTGGCGAATG ACGACTATGG AGGTGATCTG GCCTCTGTTA GCAGGCTGCT TAAGGCTCTA 
CAGGGTCTGG AGGAAATGGT AGACGCGCAC ATGGAGAAAG TCCAGGGGCT AGTGGACACT 
GCCAAAGACT TCAGCTCTCA GGGCAACTTC CTCGCAGATA ATATACAGCA GAGGGTTTGG 
GAGGTGGTCA ACAGGTATAA TGGCCTGGCT GAGCCGATGC AGACCCGCAG GGAGACTCTG 
GAGTCGTGGC AGCTGCTGTT CCAGTTCTAC AGGGACTTAG AGGATGAGCT GCTGTGGATT 
GAGGACAAGC TGCAGGCCAC GGCCACCAAA GACTGGGGCA CCTCCCTACA GAGCATACAA 
TCTATAGTCA AAAAACATCT GGTTGTGATG CAGGAAATTG AGAACCGAAC TCCTCTAATA 
ATGGCTGTAC AGGAGGCAGG ACAGAATTTG GTGCGAGGAC GACACTTTGC ATCACGTGAG 
ATCAGTGAAA AACTTTCAGG GTTAAAGAAG AACTTTGACG CCCTGAAGAA GGAGTCAGCG 
AATAAAGATC GCCTGCTTCA ACAAGCACTG AAAATCCAGA GCTTTCTCTC AGAGGTTTCC 
CAGTTGGAGC AGTGGATGGA GGAACAGAAG CCTGTTTTAG AGTCCCGCGA CTATGGAAAG 
AATGAGGAGG CCACTGAAGC TTTTCTCAGG AAGCTGGACA CAGTTGACCT GGAGTTGGAA 
AGCCAGCGTG GAAAAGTAGA GGCTCTCCTG AAAACCGGCA CAGATTTAGA GAAGTCTCAG 
CATCCCAACA GCCATTTAGT GAGTAAGAGC CTTGAAGATA TGCAGGGTGA GTTTGAGACA 
CTACTGCAAC TGTCTGGTGA ACGTCGCACA GAGCTGGAGA ATCAGTACAA TTTCTACATA 
TTTGAGAGAG ATGCCAGAGA CCTACAGACC TGGCTCCTTT CTCGGAAGAC CATAGCAGAA 
TCTGATGATT ATGGAAAAGA TCTGGAAGGA GTGGAAGCAC TGCAAAAGAA GTTTGAGGAT 
TTTTCAGAAG AGGTTGGGAC TCTGGGCCAG AGTAAGCTGA GCACTGTTCA AAAGCTAAAG 
CAAAGTGTGC AGTCAGCAGA TGCACAACAA AAAGAGAAAG ATCTACTCAA GCTCTGGGAG 
GATTTAAACA AGGCTATGAA AGCAAGAGCA GAGAACCTGC ATTCAGCTCG AGAAGTTCAC 
CAGTTTGAAC ATGACCTGGA TGAGCTTCGG ATCTGGATGA ATGAGAAGGA AGTTGCTCTG 
GACTCTGAGG AGCTGAAGAA TGACCTGCTT AGTGTCCAAG CTCTCATCCG TCAACATGAA 
GGACTGGAGA GAGACCTTGC AGTGATAGAA GAAGATGTGT CTCGTCGAAA AGAGGAGGGA 
AAAGCTTTGG TCCGCAGGTC ACCTCGAGTA AGGGACAGTT TGAGCAAGAG ACTCCAGGAA 
GTGGAGGAGA TCTGGAGAAA CCTGCTGGAG AAAGCCAACC AGCGCAGACA AAGACTCCAA 
CAGGCAGAGG CCGTTCAAAG ATATCTGATT GAATGGAGAG AGATGATGGG CTGGCTGAAA 
GAGACACTGT CTCTGATGAC AGGGGAGGGA TTCGGAGGCA AGGTTAAAGA CCTGGAACAG 
ATGATTAAAA AACATGATGA GTACCGCACA CAGATTGACA GACAACTGGA CAAATCGGAA 
ATGGTTAAAA ATGATGGGAG ACGTCTGATG GAGGAGGGGA ACTCATGGAG TCATGAGCTT 
GAGGAGCGTC TAGCCGAGCT CAAGGATCTG GAGGTGCGGG TGCTGGAGGC CTGGACTGAA 
AGGAGAGCTC AGTATCAGGA AGACATAGAG CTCCAGCAAC TGCAGAGGGA ACTGGAGCAG 
GCCGAGCACT GGCTCAACAC TTATGAGAGC GCGCTCAGAG CAGAGGACTA TGGGGAATCA 
GTTTCAGATG TTCTGGATTT GATGAAAAAG CAGGAAGATC TGGAGGCCAT GATTCAAGCA 
CAGAGTGAGA GATTCAATGC CCTTCAAGCC AGAAGAACAC AGAGGGAAAA TAAGATGAAA 
GAAATTCAAA GAGGTGACAG TACAGACAGC AGCAGGGACA AGCCTGTTCG TGTAACATCC 
CTAAAGCGAA AGCCATCTGA GCGGCCAGCA TTGCCCCCTC GTCCCTTGTT GACCAGTCCA 
GTCCTAAAGA ACAGCGATTC ACCCACACCT TCCCGAGTCC CTACCCCCAG GTCTGCCTTG 
TGGGAAAGCA GAAGTGATAA AACTGATTCT CCTGTGGTGT CACGAGTGGC AGCAGGACTC 
AGAAGAAAGA GCAGCAGTGA CAGTGATAGT GTAACATCAC CTGACAAACC ATCCACTTCT 
GGTCAGGATA GCCCTGACTC CTCCTCCACT GCGGTTTACA GACCTCATTA TCCACACAAT 
GCTTCTAAGG TTAAACATAC CACATCTTCA CAAGATGAAG TCGATACTCT AAGAGAGACT 
GAATCCTTTT TGAAAACCAT TGATAAACCA AAAATCACTC CTAGGCGTCA GAAACCATCA 
ACTCCTGATA AAGAGTTGTG GGGTCCTCCT ACATCCAAAC CACCTGAGCC ACCCATTAAA 
CCTTCACAAT CATTGTCCAG ATCTCCTCAG TCACTGTCCA GATCTCCTGA ACCACTGTCC 
AGATCTCCAG AGCCATTGCC TAGATCTCCT GAGCCACTGT CCAGATCTTC CCAGTCACAG 
CCCAGATCTC CTGAACCACT ATCCAAATCG CCTGAACCAC TATCCAGATC TCCTGAGGCA 
CTGTCCAGAT CTCCCGAGCC ACTGCCCAGA TCTCCTCAGT CACTGTCTAG ATCTCCTGAG 
CCACTGTCCA GATCTCCTGA GTCACCGTAT ATTCCTCCAC TCACAAAACC TCCATCCACA 
AACCCTCCTG ATCCACCATC CAAGGCCACA GAATTGGTGG ACAACCAAGA CTCAAAAGAT 
AGGAACCTTC CTCAATCCAC TCCACCATCA GTCCCTCAGT CATCACCTCA ATCTTTCGTT 
TCCAACCCAG AACCACAAGC GTCCTCTTCT GTGCAATCCT CAATAGTCTT TCCAGCTGCT 
TCCACCATGA TCAGAGCAGA AGATGCCAAA ATGGAGGGTA CGTTGGAGAT TAAACTGAAG 
CAAGGAGGAA ATACAGGCTT GGATCACTGG GAAACGGTCT ATGCACTACT GGAAGAGGAG 
ACACTCAATC TGTTTAAAGA CCAAGATGCC GCCACTGAGA ACTGTACACG CTGGCCACCG 
ATCAATTTGG CTGGAGCTAT TTGTAAAGAC AACCCTTTTT ACAGGAGAAA GGAGCACACG 
TTCAAACTCA TGCTGAGTGA TGGCAGTCAC TATCTGTTTG CTGCATCATC TCAAGGAGAA 
CAGCAGAAGT GGCTGAAGGA GCTCCAGGAA TGTATTAATC CAACTGTGTC TTCAGACAAT 
CCTGGAATGA AGAGAGAATC AAGCAGTGAA AGCGTAGAGA AGAGCGAGTC TGTGCCAGTC 
GACACATCTG ACACACAAGA CCAGGAGAAG GAAGACTCCA CCGAGAGCTG TGAGACAGAG 
AGAGAACCAC CACCAAAACC TCCACACACG TACTACAACA AACACAAATA CCCTGACGGA 
GGAGAGAGCC AAGACTCAGT GAGTGTGTCT CAAAATATAA AAGGGCTACA GCCTCCCACA 
TTCGCTCCTC CTCCTCCTCC AACACAAAAC CAGACAGAGA ATGAAGCCAA GGCCAAAAAC 
AGGAGCGGAT TTATGAAGTT TTTCAGAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn5 ( XM_021467434.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323109.1 Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X3, mRNA.

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CloneID	ODa149736
Clone ID Related Accession (Same CDS sequence)	XM_021467434.1
Accession Version	XM_021467434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	spectrin beta chain, non-erythrocytic 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAC GGGTAGATGG AGAGGAATCA GATGGCCGAT TTCGAGAGCT TCAGGAGCAG 
CGGATGACGG TGCAGAAGAA GACCTACACA AAATGGATGA ACAGCGTCTT CTCAAAGAGT 
GGGGTTGTCT TGACTGATGT ATTCACCGAA TTAAGAACTG GTGTGCATCT CGTGCGACTT 
CTGGAGCTCA TTTCTGGAGA GAAGTTGCCC ACTCCCAGTC GACGCACACT AAGAGTTCAC 
TGTCTGGAAA ACAACAGCAT TGCCATCACT TTCCTCAAAT CAAAGATCCG TGTAGATCTG 
ATTGGTCCAG AAAATGTGGT GGATGGAGAC AGAACCCTGA TCCTGGGTCT AATCTGGATC 
ATCATTCTCC GCTTCCAGAT TGGGAACATC AATATAGTTG AGGCCGGCGG GGATGCCAGT 
GCGGCTCGGC GCTCAGCCAA AGAAGCTCTG CTCATCTGGT GTCAACGTAA GACTGCCGGT 
TACACCAATG TAAATGTGCA AGACTTCTCA GGCAGCTGGA GAGATGGACT GGCCTTCAAT 
GCTCTAATTC ATGCCCATCG GCCTGATCTT TTTGACTACA ACCGACTCCG CCAGGATGAT 
CCAAAAAGGA GCCTCACCCA TGCTTTCGCT CTGGCAGAGG ATGAATTTGG CATAATGCAG 
CTCCTCGATG TTGAAGATGT GATGGTGCCC CATCCTGATG AGAAGTCCAT CATGACTTAC 
GTCTCTCTGT ACTATCACTA CTTCTCAAAA ATGAAGCAGG GGCAGACTAT CCAGAAGAGG 
ATAGCTAAGA TTGTGGACCT TCTGAAAGAG CTGGATGATA TGAAGGGTCT GTATGAGAGT 
ATGGTGTCTG ACTTGCTACG CTGGATCAAG ACCAAGGTGG TGGAGCTCAA TGACCGTTCC 
TTCCCCAACT CCCTGCGAGA AATGCAGCAT CTGGTAGCTG CCTTTAAGAA ATACCGTACT 
ATTGAGAAGC CTCCCAAATA CCAGGAACGG GGGGCCATTG AGGCACACCT CTTCGACTTA 
CGAACCAAGC TGAGGGCAAA CAACCAGAGG GACTACATTC CTCCGGAAGG CAAGACCTTG 
GGGGATATTG AAAGGAACTG GACCTTGCTA GAGCGAGCAG AACACCAGCG AGAACGGGTT 
CTCCAGAGTG CACTGATGAG GCTGGAGCAG CTGGAGCAGT TGGCCCAGAA GTTTGGCCGT 
AAAGCGACGC TGAGGGAGAG CTACCTGGAG GACACTCTGC AACTGATTCA ACAACAAGAC 
CTGGGAGGAC TGCAGAGGCT GGAGGAGGCT GAGATAGCAG CTCGCAGACT GGAGGCCCTG 
GGAGCTGATG TTTTGGCACA TGAACCACGT TTCCGTGCTT TAAGTGAAAT GGCTGCTGTC 
ATCGAGAGGG ATAACTACCA CAGCAAAGTA CAAGTGATTC GCAGAAATGT GGACATCATG 
AACCGCTGGA AGGATTTGAA GCAGGTTCTG CAGGAGCAGA GAGCACATGT TGGAGATGCG 
GCAAATACAT TTGCTGTCCT TAGAGACATT GAGTTAATAT CAATGGACCT CAAGGAATTA 
CAGGCTCAGG CAGATTCCTC GGACATGGGA AAACAGCTGC CAGATGTGGT AGCCCTACTG 
CAAAAGCAGG ACTTGATTGA CACTCAGATC ATCTCACTTG GTGAAACTCT GAATGCCATC 
AGTAGTAGCA GTGCCTTAAA AGTTAAACAC AAAGGTGCCA CAAAAGTCCA GAGAGAACTT 
AAAGATCTAA ATAGCCATTA CAACTCCCTA CTGGGTCTCA GCAAGAACAG GAGAAAAGCT 
CTTGAGGGAC AGCTGAAGCT CTTTGAGTTT TTCCATGATT GTGAAGAAGT AGAAGCATGG 
ATCTATGAGA AATGGGTCCT CTTACAGGCA GCATCACTAG GGAGAGACCT CAGTCAAATT 
CAGCAGGCCA TTCAGAACCA CAAGGCTTTG GAGGCAGAGA TTCAGTCACA GGAATCTCTG 
TGCTCTGGGG TTGTATTTAG AGGACAAGAG CTTTGTAGAG GGAGACACCC TAATGAGAAT 
GACATCCAAA AGTGGATTCG GACTCTTCAG AAACAGTGGC AGCAACTTAA AGAACAAGTC 
ACCATCCGAA AGAACCGGCT GCATGCTGCC AATGTCATCA AGCAGTATTT TGCAGACATT 
ACTGAAGCCA GCTCTTGGCT TGATGACCAC AAACCACTTC TCATTGACGA GGATTATGGA 
AAGGATGAGG CCAGCTCTAC TGCTCTGCTC CAGCGTCACC AACGAGTGGA GAAAGAAATG 
GAAGCATATT CCTCAGAAGT CAAACGACTG GGTGAGCAGG CCAAGAGCGC TGCCCAACTT 
GCTCCTCTTA CAACTGAAAC CCAGCAAACG AGAATGGTCC ACTACAGTGA TTCATCAGAT 
GGCGAAGATG AGAAGGAGAA AAAAGCTGAC AAAGGAGGCA AAGCAGTCAG CAAGATTTTA 
CAACAAGAAC AAGCAATGAT TAGGTTTAAA TACAGAGGCC CTAAACTAAT CTGGGACAGA 
GGAGAAATAT TGACAATTAT CAGCAGGGAA AAGGATGATA AGTTGCTTCT TCGGGACAGC 
AGAGGAAATG AGCAACTAGT CTCTTCCACA TACATCAGGG AGCTGCCAGT TTCTCAGGAA 
GCACCACCTG AAACTACAAA CGGAGTAGCT GAGAGTCCAA AAAGAAAAGT GAGCCGCCCT 
CGCCGCACTC GATCCATGCG GCGTGGCACA GCTGAAATAT CTACCTCAAC CTTACCAGAC 
CCACATTTCC AAAAAGACAC AATAGAGAGC ACACAAAACA GTCTGGAAAA GGATTTCAAC 
CGCCTTTACA ATTTAGCACA GTCAAAAAAT AAGATCCTGG AGGACACTGC ACGACTACAC 
AGATTCTACA ATGCTTGTGA GGAGTTTGAG TCCTGGATGG GAGACAAGGA GAATGTCCTC 
AACACTTTCA GCTCCAATTC CAACAACTTA GAGGTTGTAC AAGCCAAATA TGAGAATTTT 
GTAACTGAAC TAGCCAGTGG TAGAAAGCAG CTTGATGATA TTACCAAGTT TGGAGATGAG 
CTATTGAAAC AACAGCACAG CAGGAAAAAA GACATTCAGC AAAGACTTGG AATTGTTACT 
CGCAGATGGG AACGTATTCA GAAACTAAAG GATGAGATGG CTCATGAGCT TCTGAGTTCT 
GCTGATGTGA AGTCCTTCCT GCAGACCTGC CAGGAAGTCC AAGCTCAGCT CCAGCACAGA 
CTCGCCCAGC TTGACACACC TGATGTGGGG AGCTCACCAT CAGCCTTACA AGCTGAGGAA 
CACAGACAAG CCCAAGCTGA AAGGGACATA GAAGCCCTTG AAAGGAAAAT AGACTATCTT 
AAAAGTGTGG CCAAGATGAA AAAGGACTGT AGTCCTGCAG AAAGTGCTGC TATCATGGAA 
GAAGTACATG CTCTGGAAGA CTTGTTGCGG CAGTTAAAGG CTCAAGCCAT TCAAAGACAA 
CGAATGCTTG AAGAAGCCAG ACGCTTGCAA CTCTTTCAAA AAGAGACCAG AGACCTGTTG 
ATGTGGGCTG AGGCAACACA GGAGCATCTT CTGGAAGAGG AGACAGGATC TGATGTAGCA 
TCGGCTCAAG CCTTGTTGAA GGAGAACCTT GATCTCAAGC AGGAGATTGA ACTGCAGAAA 
GCAAAATTAA AAGATATGGA GAGGCTGGGT CAGTCTCTTG ACGTGGCATC TGGAGAGAAA 
GGAGGTAAAG ATGTTCAGCA GACACTTACA AAGTTGGATC ATGAGTGGAG CCAGCTGGAC 
AAACTGTGGT CTAGTCGCAA CAGAAGGCTT GAACAAGGAC TGGAGTTTCA GAAACTGAAT 
ATGGAAGCAG ATCGTATTGA GGCCACCATT TCTGGTCATG AAGCCAGACT TAAAGTCAGA 
GACTTAGGGG ACTCTGTGGA CAGTGTTCAC AGTTTATTGA GCCGACAAGA AGAGCTTGAG 
GCTTTGCTTG AGGCTCTAGA GCGGTCTATT ATCCTCTTTC AAGACAAGTG CCAGGAGCTC 
ATCAGCAAGC GTAACTTTGC TGCTAAAGAA ATTCAACAGC GATCAAAGGT CATTCAAGAT 
CATTTCAAGA GGCTGAAAGA AAGTTGTAAA TTAAGGAGGC TTGAATTACT TGCTTCGAAA 
AAGTACCAGG AATTTTTAAG AGATGCTGAG GAGATGCTGT TGTGGATGGA TGAAAAGTTT 
GAGATCGCTG AGGATGAATC GTACAGAGAT CCAACCAACA TCCTTCGTAA GCTTAAAAAA 
CATGAGGCAG CAGAAAAAGA GATGCAGGCC AATGCAGTTC GCATAGACAG ACTAATTGAG 
ACTGGAGAGA AGATGTTGGC AGATGATCAC TACAACACCC AGAGCATTCA GAAAAAGACT 
CAAGAGGTAC ACAAAAGGTG GATTGAACTT CAGAGGAAGA TGGCTGAAAG AGGGGACAAG 
CTGCGACAGG CTGGCCAACA GGAGCAACTC ATGGAGCTGC TGCAGGATGC TAAATTGAAG 
ATTGAGGCAA TTGAGAGAAT GCTACAAAAT GCAATCAGGG GTCATGACCT CCGCTCCAGC 
AGACAGCTAC TAAAAGAGCA CAAGCAGCTG GAAGAGGAAG CTCAAGAACT TGCTGAGAAA 
ATCAATTCTA TTGTCACCCG TGCCAAAAAC CTGGCCACCA ACCATTTTGA CTCTCAGCGC 
ATTCTCAAGG AGACTGACAC TTACCTGAAA CTATTTAAGT CTCTAGAGAA ACCTTTGGAT 
CATCGCAGGA GCCAGTTAGA GGCAGATGTG GATCTATACA GCTTTTACCA TGATGTAGAC 
CTAGAGCTAA ACTGGATATC AGAGCATCAG CCTGTAGCTG ATACTACAAA CTATGAGAGA 
TCACTGGTTG GGGCAGTTAG CCTTCTTCAA AAACATAAGG ACCTGCAGGC GGAGGTGAAT 
GCCCACAGCA AGTACCTGCA GCGAGTACTG GACAGAGGAC GAGCCATGGC CCGATCCAAT 
CAGTGGAATG GTCAGGAGGT GCAGCAGAGG TGTGAGCAAT TGATCGCAAA GTGGGAAGAG 
TTAGAGGAAT CCTGCAAGAA ACGCTCCGGA GAACTTAACA GGGCTGTTAC ACGGGAACAG 
ATTCTGCTTG ACTGCACTGA GCTGGAAACC CTTGTGTCAG AAACATCAGC TTTAGTCAGC 
ACTGATTATG GCAAGACTGA ATTAGCTACC CGCAGTCTAA TAAAGCAACA CGAGGGAGTG 
GAGGGGCAAA TCGAGGTACT TGCTGCTCAA GTAGATGAAC TCAAGTCCAA TGTCAAGCAG 
GCTGTACGTG TGTGGGGTTT AGAGGAGTTG AACAAGCCCT GCAACCACAT TAAATCTAAG 
CTGAGTGAGC TTCAACACTC AGCGACAGTC AGAGAACAGA GGCTTCGAGA GACTCTACAC 
CTACATGAAT TTAAGCGTGA GACTAGTGAA CTGGAGGAAT GGATTGCTCA GCAGACACAG 
ATTGCAGCCT CTAATGATCT TGGGAGTGAT TATGAAAATG CAATGCAACT GCGTGGGAGA 
TTTGAGGTCT TCTTGAAGCA GCTGGAGGTG GGAAAGGAGA GGATGCACAC CTGTGAACAG 
TTGGCTGACA AACTCATCAA AAGCAATCAC CCTGAGAGTC GCTTCATCCG AGAAACACAG 
AATCTGCTCA GAGAATCGTG GGACGAGCTG CAGGATTTAT CGAAAGATCG TAAAGAAAAG 
CTTCGTAAGT CTGAAAAATG TCACAAATTC TACAAAGACC TGACCGATGC ACTGGGACAG 
ATCAAGGAGA GGCACAAAAC CATTCCTGAA GACATAGCCA AAGATCTGAA AGGAGTGCAG 
TCACAGCTAC GCAAACATGA AGCTCTTGTG CACGAGCTGG CTGGAACTGA GCAACAGCTG 
CAGGAGCTGT TGGATGGAAC CGATGCTATT TTGGGCATGT GCTCTCCTGA GCTGCGGGTG 
GGGTTGCAGG AACTACAACA GGAGGTGGTG GAGAACTGGG AGCGGCTCCG CATGCGGATG 
GAGCAGAGAG AAGAGGAGCT GCAATCGGCT AAAGACCGCT ACATGTTCCT CAACACGGCA 
CAGGACTACT CCCTGTGGTG CTCTCAGGTG CTGAGCTGGA TGAAGGCAGA GGAGTCCATT 
CGAGACGTTG CCACCTGTGA CCTGCAGTTG TTCCAGCACC AGCAGTTGTG GGCTGAGATT 
GTGGCCCGTG AGGAGACGTT TGCCCAGGCT GTGTCAATGG GTCAAGGCCT TCTGGAGAAT 
GACATACCTA ATGCTAGAGA GATCCAGGAG AAACTGAAAG CTCTGCAAGA AGAGAGAGAG 
AAGCTTTCCG ATAACTGGCA GCTCAGGCAG AAATGGCTGG AGAACACATA TCTGGAGCAG 
GTGTTTTACA GAGACACTGA GTACATGGAG AAGATCACCA ACTCTCAAGA GATCCAGTTG 
AAGAACAGTG ATTTAGGAAC GACAGTGGAT GATGTTGAAA GTTTGATTAA GCGCCATGAG 
GCCTTTGAAA AACTTCTTCA TTCTCAGGAA GATAAGATGG TGGCTCTTCA GGAATCAGCA 
GAGCGTTTAA AGACAGAAGG TTTAACACGA GAAAAAAACA GCAATATTAA GAACAAACTC 
AAAGCTCTTC AGGAAAGGAG GGAACGGATT AAAGATCTGT CTGGCAAACG AAGAGAGGAT 
CTTGAGGTCT CAAAGCTTCA CTGTATTTTC AGCCGAGATG TGTCTGAGGC AGAGGAATGG 
ATCACAGATC GTATGAACAA GATACAAGAT GACTCCAAAA TGGACATGAG CAACCTTCAG 
ACTAAAATGA AGATGCTGCA GAAGCATCAG GTGTTTGAAG CTGAGATTCT GGCCCATGGA 
AATATCATTG AATCTGTCCA GCAGGCTGGA AATGAGCTGG TGACCATGCA TCACCCAAAA 
TCAAAGGAGA TACGCCAGAC TGTTTCAGCG CTCATCTCTC ATTGGAAGGC TCTAAAGCAA 
GAGCTTGCGG TGCGTGGAAA GGTTCTAGAG GATAACCGTG ATTTTCTTGA GTTCCTGCAG 
AAAGTGGAAC AGGTTGAAGT TTGGATTAGG CAAAAGGAGG TGATGATAAA TGTTGGAGAT 
GTCGGGGAGG ATTATGAACA CGGTCAGCAG CTGCTGAAGA AGCTCAGTGA ATTCAGAGGG 
TCAGGCTCTG GAGATGTGAC TGTTGACGAT GCTCACATCA AGACAATCAA CACTTTGGCC 
GCTCGACTTG AGAGACAGAA CAGTGATGAG CTGGTGACTG TGAAAAAGCG TAGGCAGCAG 
CTCAATGAAA GATGGAGGAA TTTCCATGGC AACCTGAGCA GCTATAAGAG GAAGCTGGAG 
GCAGCGTTGG AGGTCCACGC ATTGATCCGA GAACTAGAGG AGGTCCGGGA CAGAGCTGGA 
GAGAAGATGC TGTTGCTCCA GGGTCAGGGT TGCGGGTTGG ATGTTGAAAG TGTGGAGAAC 
CTCATTCGCA GACATGAAGA GATGGAGAGG GAGGCCAGGG TCATCCAGGA GAGAGGCGCT 
GCCCTGGAAA AGGAGACTAA AGATCGGTTG AAGAGACACT GCGATCTGTC TGACAAACTT 
AGCAAGAAGC AGGAAGAGGT CAAAATTGCT TTAGTCAAGC TGGACAAGGA AATCAAACGA 
AGGAAGGAGC GATTGCAGGA GTCTCATCAG CTCCAGCTGT TTAAAGCGAA TCAGCGTCTG 
CTTTTGGACT GGACCCTCAA GCAAAGTGAT GAGATGGTGA AGAAAGGCCT GCCTAAGAAT 
AAAACCGAAG CTGAGAGTTT CATATTAGAA CATCACGACT GGAAGGCTGA AATTGATGCT 
CGTGGCGATC GGATAGATTC AGTTAAGAGT TTCGGACAGA ATCTTGTGAA GTCTGGACAC 
AGTGATGCAG CAGAGATTAA AGAAGCCCTA CGCAAGCTGG ATGATGCCAA GACAATGCTT 
ACCCGAGCTT GGGAGGACCG AAGAAAAACT CTGGATCAAG CCCTGAAGCT CCAGATATTC 
CTGGGCTATG CTGACCAGAC TGAGAGCTGG ATGAGTAACA GGGAGGCTTT CTTGATCAAT 
GAAGATCTAG GGGGTTCATT GTCGGAGGTG GAGGCTCTAC AGAGGAAGCT GGCTCTGTTT 
GAGAACTCTC TAGAAGCTCA GATGGAGCAG GTTTCAGAGG TTGACAGATA CGCTCAGCAG 
CTCATACAGG CAAACCACTA CGACTCTGAC AACATCAAGA AGAGGACAAA AGCTATTCTG 
CTCAGGAAGG AAAGAATACT GGAAATCAGC AAATCCCGGC GAAAAGCTCT AGAAGAATCT 
CTTCAGTTGC AAAAGTTCCT GGAAGCAAGT TATGAGATTT CCTCATGGCT GAATGAGAAG 
AACTCAGTGG CTGTTGACGA GAGTTGGAAA GATCCTATAA ATCTGCAGGC TAAGCTACTG 
AAACACCAGA GCTTTGAAGC AGAGATTCTG GCCAACCGCA ACAGAGTGCA GGCACACATT 
AAAGATGGTG ATAACATGCT GGCTTTCAGT CATCCTGCTA AAAGCAAAAT AAAACCACGA 
ATAAAGGATG TCGATGACAA CTGGGACCAG CTTTTAAGCA ACTGCAAGCA TAAGAAGACA 
CGTCTACAGC AAGCGTATCA GGCCTTACAG TTCCAGCGTT CATTAGGTGA CATTGAGGAG 
TGGCTTGGGT CGGTGGAGGT GGAAGTGGCG AATGACGACT ATGGAGGTGA TCTGGCCTCT 
GTTAGCAGGC TGCTTAAGGC TCTACAGGGT CTGGAGGAAA TGGTAGACGC GCACATGGAG 
AAAGTCCAGG GGCTAGTGGA CACTGCCAAA GACTTCAGCT CTCAGGGCAA CTTCCTCGCA 
GATAATATAC AGCAGAGGGT TTGGGAGGTG GTCAACAGGT ATAATGGCCT GGCTGAGCCG 
ATGCAGACCC GCAGGGAGAC TCTGGAGTCG TGGCAGCTGC TGTTCCAGTT CTACAGGGAC 
TTAGAGGATG AGCTGCTGTG GATTGAGGAC AAGCTGCAGG CCACGGCCAC CAAAGACTGG 
GGCACCTCCC TACAGAGCAT ACAATCTATA GTCAAAAAAC ATCTGGTTGT GATGCAGGAA 
ATTGAGAACC GAACTCCTCT AATAATGGCT GTACAGGAGG CAGGACAGAA TTTGGTGCGA 
GGACGACACT TTGCATCACG TGAGATCAGT GAAAAACTTT CAGGGTTAAA GAAGAACTTT 
GACGCCCTGA AGAAGGAGTC AGCGAATAAA GATCGCCTGC TTCAACAAGC ACTGAAAATC 
CAGAGCTTTC TCTCAGAGGT TTCCCAGTTG GAGCAGTGGA TGGAGGAACA GAAGCCTGTT 
TTAGAGTCCC GCGACTATGG AAAGAATGAG GAGGCCACTG AAGCTTTTCT CAGGAAGCTG 
GACACAGTTG ACCTGGAGTT GGAAAGCCAG CGTGGAAAAG TAGAGGCTCT CCTGAAAACC 
GGCACAGATT TAGAGAAGTC TCAGCATCCC AACAGCCATT TAGTGAGTAA GAGCCTTGAA 
GATATGCAGG GTGAGTTTGA GACACTACTG CAACTGTCTG GTGAACGTCG CACAGAGCTG 
GAGAATCAGT ACAATTTCTA CATATTTGAG AGAGATGCCA GAGACCTACA GACCTGGCTC 
CTTTCTCGGA AGACCATAGC AGAATCTGAT GATTATGGAA AAGATCTGGA AGGAGTGGAA 
GCACTGCAAA AGAAGTTTGA GGATTTTTCA GAAGAGGTTG GGACTCTGGG CCAGAGTAAG 
CTGAGCACTG TTCAAAAGCT AAAGCAAAGT GTGCAGTCAG CAGATGCACA ACAAAAAGAG 
AAAGATCTAC TCAAGCTCTG GGAGGATTTA AACAAGGCTA TGAAAGCAAG AGCAGAGAAC 
CTGCATTCAG CTCGAGAAGT TCACCAGTTT GAACATGACC TGGATGAGCT TCGGATCTGG 
ATGAATGAGA AGGAAGTTGC TCTGGACTCT GAGGAGCTGA AGAATGACCT GCTTAGTGTC 
CAAGCTCTCA TCCGTCAACA TGAAGGACTG GAGAGAGACC TTGCAGTGAT AGAAGAAGAT 
GTGTCTCGTC GAAAAGAGGA GGGAAAAGCT TTGGTCCGCA GGTCACCTCG AGTAAGGGAC 
AGTTTGAGCA AGAGACTCCA GGAAGTGGAG GAGATCTGGA GAAACCTGCT GGAGAAAGCC 
AACCAGCGCA GACAAAGACT CCAACAGGCA GAGGCCGTTC AAAGATATCT GATTGAATGG 
AGAGAGATGA TGGGCTGGCT GAAAGAGACA CTGTCTCTGA TGACAGGGGA GGGATTCGGA 
GGCAAGGTTA AAGACCTGGA ACAGATGATT AAAAAACATG ATGAGTACCG CACACAGATT 
GACAGACAAC TGGACAAATC GGAAATGGTT AAAAATGATG GGAGACGTCT GATGGAGGAG 
GGGAACTCAT GGAGTCATGA GCTTGAGGAG CGTCTAGCCG AGCTCAAGGA TCTGGAGGTG 
CGGGTGCTGG AGGCCTGGAC TGAAAGGAGA GCTCAGTATC AGGAAGACAT AGAGCTCCAG 
CAACTGCAGA GGGAACTGGA GCAGGCCGAG CACTGGCTCA ACACTTATGA GAGCGCGCTC 
AGAGCAGAGG ACTATGGGGA ATCAGTTTCA GATGTTCTGG ATTTGATGAA AAAGCAGGAA 
GATCTGGAGG CCATGATTCA AGCACAGAGT GAGAGATTCA ATGCCCTTCA AGCCAGAAGA 
ACACAGAGGG AAAATAAGAT GAAAGAAATT CAAAGAGGTG ACAGTACAGA CAGCAGCAGG 
GACAAGCCTG TTCGTGTAAC ATCCCTAAAG CGAAAGCCAT CTGAGCGGCC AGCATTGCCC 
CCTCGTCCCT TGTTGACCAG TCCAGTCCTA AAGAACAGCG ATTCACCCAC ACCTTCCCGA 
GTCCCTACCC CCAGGTCTGC CTTGTGGGAA AGCAGAAGTG ATAAAACTGA TTCTCCTGTG 
GTGTCACGAG TGGCAGCAGG ACTCAGAAGA AAGAGCAGCA GTGACAGTGA TAGTGTAACA 
TCACCTGACA AACCATCCAC TTCTGGTCAG GATAGCCCTG ACTCCTCCTC CACTGCGGTT 
TACAGACCTC ATTATCCACA CAATGCTTCT AAGGTTAAAC ATACCACATC TTCACAAGAT 
GAAGTCGATA CTCTAAGAGA GACTGAATCC TTTTTGAAAA CCATTGATAA ACCAAAAATC 
ACTCCTAGGC GTCAGAAACC ATCAACTCCT GATAAAGAGT TGTGGGGTCC TCCTACATCC 
AAACCACCTG AGCCACCCAT TAAACCTTCA CAATCATTGT CCAGATCTCC TCAGTCACTG 
TCCAGATCTC CTGAACCACT GTCCAGATCT CCAGAGCCAT TGCCTAGATC TCCTGAGCCA 
CTGTCCAGAT CTTCCCAGTC ACAGCCCAGA TCTCCTGAAC CACTATCCAA ATCGCCTGAA 
CCACTATCCA GATCTCCTGA GGCACTGTCC AGATCTCCCG AGCCACTGCC CAGATCTCCT 
CAGTCACTGT CTAGATCTCC TGAGCCACTG TCCAGATCTC CTGAGTCACC GTATATTCCT 
CCACTCACAA AACCTCCATC CACAAACCCT CCTGATCCAC CATCCAAGGC CACAGAATTG 
GTGGACAACC AAGACTCAAA AGATAGGAAC CTTCCTCAAT CCACTCCACC ATCAGTCCCT 
CAGTCATCAC CTCAATCTTT CGTTTCCAAC CCAGAACCAC AAGCGTCCTC TTCTGTGCAA 
TCCTCAATAG TCTTTCCAGC TGCTTCCACC ATGATCAGAG CAGAAGATGC CAAAATGGAG 
GGTACGTTGG AGATTAAACT GAAGCAAGGA GGAAATACAG GCTTGGATCA CTGGGAAACG 
GTCTATGCAC TACTGGAAGA GGAGACACTC AATCTGTTTA AAGACCAAGA TGCCGCCACT 
GAGAACTGTA CACGCTGGCC ACCGATCAAT TTGGCTGGAG CTATTTGTAA AGACAACCCT 
TTTTACAGGA GAAAGGAGCA CACGTTCAAA CTCATGCTGA GTGATGGCAG TCACTATCTG 
TTTGCTGCAT CATCTCAAGG AGAACAGCAG AAGTGGCTGA AGGAGCTCCA GGAATGTATT 
AATCCAACTG TGTCTTCAGA CAATCCTGGA ATGAAGAGAG AATCAAGCAG TGAAAGCGTA 
GAGAAGAGCG AGTCTGTGCC AGTCGACACA TCTGACACAC AAGACCAGGA GAAGGAAGAC 
TCCACCGAGA GCTGTGAGAC AGAGAGAGAA CCACCACCAA AACCTCCACA CACGTACTAC 
AACAAACACA AATACCCTGA CGGAGGAGAG AGCCAAGACT CAGTGAGTGT GTCTCAAAAT 
ATAAAAGGGC TACAGCCTCC CACATTCGCT CCTCCTCCTC CTCCAACACA AAACCAGACA 
GAGAATGAAG CCAAGGCCAA AAACAGGAGC GGATTTATGA AGTTTTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323109.1
CDS	165..12581
Translation	MEKRVDGEESDGRFRELQEQRMTVQKKTYTKWMNSVFSKSGVVLTDVFTELRTGVHLVRLLELISGEKLPTPSRRTLRVHCLENNSIAITFLKSKIRVDLIGPENVVDGDRTLILGLIWIIILRFQIGNINIVEAGGDASAARRSAKEALLIWCQRKTAGYTNVNVQDFSGSWRDGLAFNALIHAHRPDLFDYNRLRQDDPKRSLTHAFALAEDEFGIMQLLDVEDVMVPHPDEKSIMTYVSLYYHYFSKMKQGQTIQKRIAKIVDLLKELDDMKGLYESMVSDLLRWIKTKVVELNDRSFPNSLREMQHLVAAFKKYRTIEKPPKYQERGAIEAHLFDLRTKLRANNQRDYIPPEGKTLGDIERNWTLLERAEHQRERVLQSALMRLEQLEQLAQKFGRKATLRESYLEDTLQLIQQQDLGGLQRLEEAEIAARRLEALGADVLAHEPRFRALSEMAAVIERDNYHSKVQVIRRNVDIMNRWKDLKQVLQEQRAHVGDAANTFAVLRDIELISMDLKELQAQADSSDMGKQLPDVVALLQKQDLIDTQIISLGETLNAISSSSALKVKHKGATKVQRELKDLNSHYNSLLGLSKNRRKALEGQLKLFEFFHDCEEVEAWIYEKWVLLQAASLGRDLSQIQQAIQNHKALEAEIQSQESLCSGVVFRGQELCRGRHPNENDIQKWIRTLQKQWQQLKEQVTIRKNRLHAANVIKQYFADITEASSWLDDHKPLLIDEDYGKDEASSTALLQRHQRVEKEMEAYSSEVKRLGEQAKSAAQLAPLTTETQQTRMVHYSDSSDGEDEKEKKADKGGKAVSKILQQEQAMIRFKYRGPKLIWDRGEILTIISREKDDKLLLRDSRGNEQLVSSTYIRELPVSQEAPPETTNGVAESPKRKVSRPRRTRSMRRGTAEISTSTLPDPHFQKDTIESTQNSLEKDFNRLYNLAQSKNKILEDTARLHRFYNACEEFESWMGDKENVLNTFSSNSNNLEVVQAKYENFVTELASGRKQLDDITKFGDELLKQQHSRKKDIQQRLGIVTRRWERIQKLKDEMAHELLSSADVKSFLQTCQEVQAQLQHRLAQLDTPDVGSSPSALQAEEHRQAQAERDIEALERKIDYLKSVAKMKKDCSPAESAAIMEEVHALEDLLRQLKAQAIQRQRMLEEARRLQLFQKETRDLLMWAEATQEHLLEEETGSDVASAQALLKENLDLKQEIELQKAKLKDMERLGQSLDVASGEKGGKDVQQTLTKLDHEWSQLDKLWSSRNRRLEQGLEFQKLNMEADRIEATISGHEARLKVRDLGDSVDSVHSLLSRQEELEALLEALERSIILFQDKCQELISKRNFAAKEIQQRSKVIQDHFKRLKESCKLRRLELLASKKYQEFLRDAEEMLLWMDEKFEIAEDESYRDPTNILRKLKKHEAAEKEMQANAVRIDRLIETGEKMLADDHYNTQSIQKKTQEVHKRWIELQRKMAERGDKLRQAGQQEQLMELLQDAKLKIEAIERMLQNAIRGHDLRSSRQLLKEHKQLEEEAQELAEKINSIVTRAKNLATNHFDSQRILKETDTYLKLFKSLEKPLDHRRSQLEADVDLYSFYHDVDLELNWISEHQPVADTTNYERSLVGAVSLLQKHKDLQAEVNAHSKYLQRVLDRGRAMARSNQWNGQEVQQRCEQLIAKWEELEESCKKRSGELNRAVTREQILLDCTELETLVSETSALVSTDYGKTELATRSLIKQHEGVEGQIEVLAAQVDELKSNVKQAVRVWGLEELNKPCNHIKSKLSELQHSATVREQRLRETLHLHEFKRETSELEEWIAQQTQIAASNDLGSDYENAMQLRGRFEVFLKQLEVGKERMHTCEQLADKLIKSNHPESRFIRETQNLLRESWDELQDLSKDRKEKLRKSEKCHKFYKDLTDALGQIKERHKTIPEDIAKDLKGVQSQLRKHEALVHELAGTEQQLQELLDGTDAILGMCSPELRVGLQELQQEVVENWERLRMRMEQREEELQSAKDRYMFLNTAQDYSLWCSQVLSWMKAEESIRDVATCDLQLFQHQQLWAEIVAREETFAQAVSMGQGLLENDIPNAREIQEKLKALQEEREKLSDNWQLRQKWLENTYLEQVFYRDTEYMEKITNSQEIQLKNSDLGTTVDDVESLIKRHEAFEKLLHSQEDKMVALQESAERLKTEGLTREKNSNIKNKLKALQERRERIKDLSGKRREDLEVSKLHCIFSRDVSEAEEWITDRMNKIQDDSKMDMSNLQTKMKMLQKHQVFEAEILAHGNIIESVQQAGNELVTMHHPKSKEIRQTVSALISHWKALKQELAVRGKVLEDNRDFLEFLQKVEQVEVWIRQKEVMINVGDVGEDYEHGQQLLKKLSEFRGSGSGDVTVDDAHIKTINTLAARLERQNSDELVTVKKRRQQLNERWRNFHGNLSSYKRKLEAALEVHALIRELEEVRDRAGEKMLLLQGQGCGLDVESVENLIRRHEEMEREARVIQERGAALEKETKDRLKRHCDLSDKLSKKQEEVKIALVKLDKEIKRRKERLQESHQLQLFKANQRLLLDWTLKQSDEMVKKGLPKNKTEAESFILEHHDWKAEIDARGDRIDSVKSFGQNLVKSGHSDAAEIKEALRKLDDAKTMLTRAWEDRRKTLDQALKLQIFLGYADQTESWMSNREAFLINEDLGGSLSEVEALQRKLALFENSLEAQMEQVSEVDRYAQQLIQANHYDSDNIKKRTKAILLRKERILEISKSRRKALEESLQLQKFLEASYEISSWLNEKNSVAVDESWKDPINLQAKLLKHQSFEAEILANRNRVQAHIKDGDNMLAFSHPAKSKIKPRIKDVDDNWDQLLSNCKHKKTRLQQAYQALQFQRSLGDIEEWLGSVEVEVANDDYGGDLASVSRLLKALQGLEEMVDAHMEKVQGLVDTAKDFSSQGNFLADNIQQRVWEVVNRYNGLAEPMQTRRETLESWQLLFQFYRDLEDELLWIEDKLQATATKDWGTSLQSIQSIVKKHLVVMQEIENRTPLIMAVQEAGQNLVRGRHFASREISEKLSGLKKNFDALKKESANKDRLLQQALKIQSFLSEVSQLEQWMEEQKPVLESRDYGKNEEATEAFLRKLDTVDLELESQRGKVEALLKTGTDLEKSQHPNSHLVSKSLEDMQGEFETLLQLSGERRTELENQYNFYIFERDARDLQTWLLSRKTIAESDDYGKDLEGVEALQKKFEDFSEEVGTLGQSKLSTVQKLKQSVQSADAQQKEKDLLKLWEDLNKAMKARAENLHSAREVHQFEHDLDELRIWMNEKEVALDSEELKNDLLSVQALIRQHEGLERDLAVIEEDVSRRKEEGKALVRRSPRVRDSLSKRLQEVEEIWRNLLEKANQRRQRLQQAEAVQRYLIEWREMMGWLKETLSLMTGEGFGGKVKDLEQMIKKHDEYRTQIDRQLDKSEMVKNDGRRLMEEGNSWSHELEERLAELKDLEVRVLEAWTERRAQYQEDIELQQLQRELEQAEHWLNTYESALRAEDYGESVSDVLDLMKKQEDLEAMIQAQSERFNALQARRTQRENKMKEIQRGDSTDSSRDKPVRVTSLKRKPSERPALPPRPLLTSPVLKNSDSPTPSRVPTPRSALWESRSDKTDSPVVSRVAAGLRRKSSSDSDSVTSPDKPSTSGQDSPDSSSTAVYRPHYPHNASKVKHTTSSQDEVDTLRETESFLKTIDKPKITPRRQKPSTPDKELWGPPTSKPPEPPIKPSQSLSRSPQSLSRSPEPLSRSPEPLPRSPEPLSRSSQSQPRSPEPLSKSPEPLSRSPEALSRSPEPLPRSPQSLSRSPEPLSRSPESPYIPPLTKPPSTNPPDPPSKATELVDNQDSKDRNLPQSTPPSVPQSSPQSFVSNPEPQASSSVQSSIVFPAASTMIRAEDAKMEGTLEIKLKQGGNTGLDHWETVYALLEEETLNLFKDQDAATENCTRWPPINLAGAICKDNPFYRRKEHTFKLMLSDGSHYLFAASSQGEQQKWLKELQECINPTVSSDNPGMKRESSSESVEKSESVPVDTSDTQDQEKEDSTESCETEREPPPKPPHTYYNKHKYPDGGESQDSVSVSQNIKGLQPPTFAPPPPPTQNQTENEAKAKNRSGFMKFFRK

Target ORF information:

RefSeq Version	XM_021467434.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467434.1

ORF Insert Sequence:

ATGGAAAAAC GGGTAGATGG AGAGGAATCA GATGGCCGAT TTCGAGAGCT TCAGGAGCAG 
CGGATGACGG TGCAGAAGAA GACCTACACA AAATGGATGA ACAGCGTCTT CTCAAAGAGT 
GGGGTTGTCT TGACTGATGT ATTCACCGAA TTAAGAACTG GTGTGCATCT CGTGCGACTT 
CTGGAGCTCA TTTCTGGAGA GAAGTTGCCC ACTCCCAGTC GACGCACACT AAGAGTTCAC 
TGTCTGGAAA ACAACAGCAT TGCCATCACT TTCCTCAAAT CAAAGATCCG TGTAGATCTG 
ATTGGTCCAG AAAATGTGGT GGATGGAGAC AGAACCCTGA TCCTGGGTCT AATCTGGATC 
ATCATTCTCC GCTTCCAGAT TGGGAACATC AATATAGTTG AGGCCGGCGG GGATGCCAGT 
GCGGCTCGGC GCTCAGCCAA AGAAGCTCTG CTCATCTGGT GTCAACGTAA GACTGCCGGT 
TACACCAATG TAAATGTGCA AGACTTCTCA GGCAGCTGGA GAGATGGACT GGCCTTCAAT 
GCTCTAATTC ATGCCCATCG GCCTGATCTT TTTGACTACA ACCGACTCCG CCAGGATGAT 
CCAAAAAGGA GCCTCACCCA TGCTTTCGCT CTGGCAGAGG ATGAATTTGG CATAATGCAG 
CTCCTCGATG TTGAAGATGT GATGGTGCCC CATCCTGATG AGAAGTCCAT CATGACTTAC 
GTCTCTCTGT ACTATCACTA CTTCTCAAAA ATGAAGCAGG GGCAGACTAT CCAGAAGAGG 
ATAGCTAAGA TTGTGGACCT TCTGAAAGAG CTGGATGATA TGAAGGGTCT GTATGAGAGT 
ATGGTGTCTG ACTTGCTACG CTGGATCAAG ACCAAGGTGG TGGAGCTCAA TGACCGTTCC 
TTCCCCAACT CCCTGCGAGA AATGCAGCAT CTGGTAGCTG CCTTTAAGAA ATACCGTACT 
ATTGAGAAGC CTCCCAAATA CCAGGAACGG GGGGCCATTG AGGCACACCT CTTCGACTTA 
CGAACCAAGC TGAGGGCAAA CAACCAGAGG GACTACATTC CTCCGGAAGG CAAGACCTTG 
GGGGATATTG AAAGGAACTG GACCTTGCTA GAGCGAGCAG AACACCAGCG AGAACGGGTT 
CTCCAGAGTG CACTGATGAG GCTGGAGCAG CTGGAGCAGT TGGCCCAGAA GTTTGGCCGT 
AAAGCGACGC TGAGGGAGAG CTACCTGGAG GACACTCTGC AACTGATTCA ACAACAAGAC 
CTGGGAGGAC TGCAGAGGCT GGAGGAGGCT GAGATAGCAG CTCGCAGACT GGAGGCCCTG 
GGAGCTGATG TTTTGGCACA TGAACCACGT TTCCGTGCTT TAAGTGAAAT GGCTGCTGTC 
ATCGAGAGGG ATAACTACCA CAGCAAAGTA CAAGTGATTC GCAGAAATGT GGACATCATG 
AACCGCTGGA AGGATTTGAA GCAGGTTCTG CAGGAGCAGA GAGCACATGT TGGAGATGCG 
GCAAATACAT TTGCTGTCCT TAGAGACATT GAGTTAATAT CAATGGACCT CAAGGAATTA 
CAGGCTCAGG CAGATTCCTC GGACATGGGA AAACAGCTGC CAGATGTGGT AGCCCTACTG 
CAAAAGCAGG ACTTGATTGA CACTCAGATC ATCTCACTTG GTGAAACTCT GAATGCCATC 
AGTAGTAGCA GTGCCTTAAA AGTTAAACAC AAAGGTGCCA CAAAAGTCCA GAGAGAACTT 
AAAGATCTAA ATAGCCATTA CAACTCCCTA CTGGGTCTCA GCAAGAACAG GAGAAAAGCT 
CTTGAGGGAC AGCTGAAGCT CTTTGAGTTT TTCCATGATT GTGAAGAAGT AGAAGCATGG 
ATCTATGAGA AATGGGTCCT CTTACAGGCA GCATCACTAG GGAGAGACCT CAGTCAAATT 
CAGCAGGCCA TTCAGAACCA CAAGGCTTTG GAGGCAGAGA TTCAGTCACA GGAATCTCTG 
TGCTCTGGGG TTGTATTTAG AGGACAAGAG CTTTGTAGAG GGAGACACCC TAATGAGAAT 
GACATCCAAA AGTGGATTCG GACTCTTCAG AAACAGTGGC AGCAACTTAA AGAACAAGTC 
ACCATCCGAA AGAACCGGCT GCATGCTGCC AATGTCATCA AGCAGTATTT TGCAGACATT 
ACTGAAGCCA GCTCTTGGCT TGATGACCAC AAACCACTTC TCATTGACGA GGATTATGGA 
AAGGATGAGG CCAGCTCTAC TGCTCTGCTC CAGCGTCACC AACGAGTGGA GAAAGAAATG 
GAAGCATATT CCTCAGAAGT CAAACGACTG GGTGAGCAGG CCAAGAGCGC TGCCCAACTT 
GCTCCTCTTA CAACTGAAAC CCAGCAAACG AGAATGGTCC ACTACAGTGA TTCATCAGAT 
GGCGAAGATG AGAAGGAGAA AAAAGCTGAC AAAGGAGGCA AAGCAGTCAG CAAGATTTTA 
CAACAAGAAC AAGCAATGAT TAGGTTTAAA TACAGAGGCC CTAAACTAAT CTGGGACAGA 
GGAGAAATAT TGACAATTAT CAGCAGGGAA AAGGATGATA AGTTGCTTCT TCGGGACAGC 
AGAGGAAATG AGCAACTAGT CTCTTCCACA TACATCAGGG AGCTGCCAGT TTCTCAGGAA 
GCACCACCTG AAACTACAAA CGGAGTAGCT GAGAGTCCAA AAAGAAAAGT GAGCCGCCCT 
CGCCGCACTC GATCCATGCG GCGTGGCACA GCTGAAATAT CTACCTCAAC CTTACCAGAC 
CCACATTTCC AAAAAGACAC AATAGAGAGC ACACAAAACA GTCTGGAAAA GGATTTCAAC 
CGCCTTTACA ATTTAGCACA GTCAAAAAAT AAGATCCTGG AGGACACTGC ACGACTACAC 
AGATTCTACA ATGCTTGTGA GGAGTTTGAG TCCTGGATGG GAGACAAGGA GAATGTCCTC 
AACACTTTCA GCTCCAATTC CAACAACTTA GAGGTTGTAC AAGCCAAATA TGAGAATTTT 
GTAACTGAAC TAGCCAGTGG TAGAAAGCAG CTTGATGATA TTACCAAGTT TGGAGATGAG 
CTATTGAAAC AACAGCACAG CAGGAAAAAA GACATTCAGC AAAGACTTGG AATTGTTACT 
CGCAGATGGG AACGTATTCA GAAACTAAAG GATGAGATGG CTCATGAGCT TCTGAGTTCT 
GCTGATGTGA AGTCCTTCCT GCAGACCTGC CAGGAAGTCC AAGCTCAGCT CCAGCACAGA 
CTCGCCCAGC TTGACACACC TGATGTGGGG AGCTCACCAT CAGCCTTACA AGCTGAGGAA 
CACAGACAAG CCCAAGCTGA AAGGGACATA GAAGCCCTTG AAAGGAAAAT AGACTATCTT 
AAAAGTGTGG CCAAGATGAA AAAGGACTGT AGTCCTGCAG AAAGTGCTGC TATCATGGAA 
GAAGTACATG CTCTGGAAGA CTTGTTGCGG CAGTTAAAGG CTCAAGCCAT TCAAAGACAA 
CGAATGCTTG AAGAAGCCAG ACGCTTGCAA CTCTTTCAAA AAGAGACCAG AGACCTGTTG 
ATGTGGGCTG AGGCAACACA GGAGCATCTT CTGGAAGAGG AGACAGGATC TGATGTAGCA 
TCGGCTCAAG CCTTGTTGAA GGAGAACCTT GATCTCAAGC AGGAGATTGA ACTGCAGAAA 
GCAAAATTAA AAGATATGGA GAGGCTGGGT CAGTCTCTTG ACGTGGCATC TGGAGAGAAA 
GGAGGTAAAG ATGTTCAGCA GACACTTACA AAGTTGGATC ATGAGTGGAG CCAGCTGGAC 
AAACTGTGGT CTAGTCGCAA CAGAAGGCTT GAACAAGGAC TGGAGTTTCA GAAACTGAAT 
ATGGAAGCAG ATCGTATTGA GGCCACCATT TCTGGTCATG AAGCCAGACT TAAAGTCAGA 
GACTTAGGGG ACTCTGTGGA CAGTGTTCAC AGTTTATTGA GCCGACAAGA AGAGCTTGAG 
GCTTTGCTTG AGGCTCTAGA GCGGTCTATT ATCCTCTTTC AAGACAAGTG CCAGGAGCTC 
ATCAGCAAGC GTAACTTTGC TGCTAAAGAA ATTCAACAGC GATCAAAGGT CATTCAAGAT 
CATTTCAAGA GGCTGAAAGA AAGTTGTAAA TTAAGGAGGC TTGAATTACT TGCTTCGAAA 
AAGTACCAGG AATTTTTAAG AGATGCTGAG GAGATGCTGT TGTGGATGGA TGAAAAGTTT 
GAGATCGCTG AGGATGAATC GTACAGAGAT CCAACCAACA TCCTTCGTAA GCTTAAAAAA 
CATGAGGCAG CAGAAAAAGA GATGCAGGCC AATGCAGTTC GCATAGACAG ACTAATTGAG 
ACTGGAGAGA AGATGTTGGC AGATGATCAC TACAACACCC AGAGCATTCA GAAAAAGACT 
CAAGAGGTAC ACAAAAGGTG GATTGAACTT CAGAGGAAGA TGGCTGAAAG AGGGGACAAG 
CTGCGACAGG CTGGCCAACA GGAGCAACTC ATGGAGCTGC TGCAGGATGC TAAATTGAAG 
ATTGAGGCAA TTGAGAGAAT GCTACAAAAT GCAATCAGGG GTCATGACCT CCGCTCCAGC 
AGACAGCTAC TAAAAGAGCA CAAGCAGCTG GAAGAGGAAG CTCAAGAACT TGCTGAGAAA 
ATCAATTCTA TTGTCACCCG TGCCAAAAAC CTGGCCACCA ACCATTTTGA CTCTCAGCGC 
ATTCTCAAGG AGACTGACAC TTACCTGAAA CTATTTAAGT CTCTAGAGAA ACCTTTGGAT 
CATCGCAGGA GCCAGTTAGA GGCAGATGTG GATCTATACA GCTTTTACCA TGATGTAGAC 
CTAGAGCTAA ACTGGATATC AGAGCATCAG CCTGTAGCTG ATACTACAAA CTATGAGAGA 
TCACTGGTTG GGGCAGTTAG CCTTCTTCAA AAACATAAGG ACCTGCAGGC GGAGGTGAAT 
GCCCACAGCA AGTACCTGCA GCGAGTACTG GACAGAGGAC GAGCCATGGC CCGATCCAAT 
CAGTGGAATG GTCAGGAGGT GCAGCAGAGG TGTGAGCAAT TGATCGCAAA GTGGGAAGAG 
TTAGAGGAAT CCTGCAAGAA ACGCTCCGGA GAACTTAACA GGGCTGTTAC ACGGGAACAG 
ATTCTGCTTG ACTGCACTGA GCTGGAAACC CTTGTGTCAG AAACATCAGC TTTAGTCAGC 
ACTGATTATG GCAAGACTGA ATTAGCTACC CGCAGTCTAA TAAAGCAACA CGAGGGAGTG 
GAGGGGCAAA TCGAGGTACT TGCTGCTCAA GTAGATGAAC TCAAGTCCAA TGTCAAGCAG 
GCTGTACGTG TGTGGGGTTT AGAGGAGTTG AACAAGCCCT GCAACCACAT TAAATCTAAG 
CTGAGTGAGC TTCAACACTC AGCGACAGTC AGAGAACAGA GGCTTCGAGA GACTCTACAC 
CTACATGAAT TTAAGCGTGA GACTAGTGAA CTGGAGGAAT GGATTGCTCA GCAGACACAG 
ATTGCAGCCT CTAATGATCT TGGGAGTGAT TATGAAAATG CAATGCAACT GCGTGGGAGA 
TTTGAGGTCT TCTTGAAGCA GCTGGAGGTG GGAAAGGAGA GGATGCACAC CTGTGAACAG 
TTGGCTGACA AACTCATCAA AAGCAATCAC CCTGAGAGTC GCTTCATCCG AGAAACACAG 
AATCTGCTCA GAGAATCGTG GGACGAGCTG CAGGATTTAT CGAAAGATCG TAAAGAAAAG 
CTTCGTAAGT CTGAAAAATG TCACAAATTC TACAAAGACC TGACCGATGC ACTGGGACAG 
ATCAAGGAGA GGCACAAAAC CATTCCTGAA GACATAGCCA AAGATCTGAA AGGAGTGCAG 
TCACAGCTAC GCAAACATGA AGCTCTTGTG CACGAGCTGG CTGGAACTGA GCAACAGCTG 
CAGGAGCTGT TGGATGGAAC CGATGCTATT TTGGGCATGT GCTCTCCTGA GCTGCGGGTG 
GGGTTGCAGG AACTACAACA GGAGGTGGTG GAGAACTGGG AGCGGCTCCG CATGCGGATG 
GAGCAGAGAG AAGAGGAGCT GCAATCGGCT AAAGACCGCT ACATGTTCCT CAACACGGCA 
CAGGACTACT CCCTGTGGTG CTCTCAGGTG CTGAGCTGGA TGAAGGCAGA GGAGTCCATT 
CGAGACGTTG CCACCTGTGA CCTGCAGTTG TTCCAGCACC AGCAGTTGTG GGCTGAGATT 
GTGGCCCGTG AGGAGACGTT TGCCCAGGCT GTGTCAATGG GTCAAGGCCT TCTGGAGAAT 
GACATACCTA ATGCTAGAGA GATCCAGGAG AAACTGAAAG CTCTGCAAGA AGAGAGAGAG 
AAGCTTTCCG ATAACTGGCA GCTCAGGCAG AAATGGCTGG AGAACACATA TCTGGAGCAG 
GTGTTTTACA GAGACACTGA GTACATGGAG AAGATCACCA ACTCTCAAGA GATCCAGTTG 
AAGAACAGTG ATTTAGGAAC GACAGTGGAT GATGTTGAAA GTTTGATTAA GCGCCATGAG 
GCCTTTGAAA AACTTCTTCA TTCTCAGGAA GATAAGATGG TGGCTCTTCA GGAATCAGCA 
GAGCGTTTAA AGACAGAAGG TTTAACACGA GAAAAAAACA GCAATATTAA GAACAAACTC 
AAAGCTCTTC AGGAAAGGAG GGAACGGATT AAAGATCTGT CTGGCAAACG AAGAGAGGAT 
CTTGAGGTCT CAAAGCTTCA CTGTATTTTC AGCCGAGATG TGTCTGAGGC AGAGGAATGG 
ATCACAGATC GTATGAACAA GATACAAGAT GACTCCAAAA TGGACATGAG CAACCTTCAG 
ACTAAAATGA AGATGCTGCA GAAGCATCAG GTGTTTGAAG CTGAGATTCT GGCCCATGGA 
AATATCATTG AATCTGTCCA GCAGGCTGGA AATGAGCTGG TGACCATGCA TCACCCAAAA 
TCAAAGGAGA TACGCCAGAC TGTTTCAGCG CTCATCTCTC ATTGGAAGGC TCTAAAGCAA 
GAGCTTGCGG TGCGTGGAAA GGTTCTAGAG GATAACCGTG ATTTTCTTGA GTTCCTGCAG 
AAAGTGGAAC AGGTTGAAGT TTGGATTAGG CAAAAGGAGG TGATGATAAA TGTTGGAGAT 
GTCGGGGAGG ATTATGAACA CGGTCAGCAG CTGCTGAAGA AGCTCAGTGA ATTCAGAGGG 
TCAGGCTCTG GAGATGTGAC TGTTGACGAT GCTCACATCA AGACAATCAA CACTTTGGCC 
GCTCGACTTG AGAGACAGAA CAGTGATGAG CTGGTGACTG TGAAAAAGCG TAGGCAGCAG 
CTCAATGAAA GATGGAGGAA TTTCCATGGC AACCTGAGCA GCTATAAGAG GAAGCTGGAG 
GCAGCGTTGG AGGTCCACGC ATTGATCCGA GAACTAGAGG AGGTCCGGGA CAGAGCTGGA 
GAGAAGATGC TGTTGCTCCA GGGTCAGGGT TGCGGGTTGG ATGTTGAAAG TGTGGAGAAC 
CTCATTCGCA GACATGAAGA GATGGAGAGG GAGGCCAGGG TCATCCAGGA GAGAGGCGCT 
GCCCTGGAAA AGGAGACTAA AGATCGGTTG AAGAGACACT GCGATCTGTC TGACAAACTT 
AGCAAGAAGC AGGAAGAGGT CAAAATTGCT TTAGTCAAGC TGGACAAGGA AATCAAACGA 
AGGAAGGAGC GATTGCAGGA GTCTCATCAG CTCCAGCTGT TTAAAGCGAA TCAGCGTCTG 
CTTTTGGACT GGACCCTCAA GCAAAGTGAT GAGATGGTGA AGAAAGGCCT GCCTAAGAAT 
AAAACCGAAG CTGAGAGTTT CATATTAGAA CATCACGACT GGAAGGCTGA AATTGATGCT 
CGTGGCGATC GGATAGATTC AGTTAAGAGT TTCGGACAGA ATCTTGTGAA GTCTGGACAC 
AGTGATGCAG CAGAGATTAA AGAAGCCCTA CGCAAGCTGG ATGATGCCAA GACAATGCTT 
ACCCGAGCTT GGGAGGACCG AAGAAAAACT CTGGATCAAG CCCTGAAGCT CCAGATATTC 
CTGGGCTATG CTGACCAGAC TGAGAGCTGG ATGAGTAACA GGGAGGCTTT CTTGATCAAT 
GAAGATCTAG GGGGTTCATT GTCGGAGGTG GAGGCTCTAC AGAGGAAGCT GGCTCTGTTT 
GAGAACTCTC TAGAAGCTCA GATGGAGCAG GTTTCAGAGG TTGACAGATA CGCTCAGCAG 
CTCATACAGG CAAACCACTA CGACTCTGAC AACATCAAGA AGAGGACAAA AGCTATTCTG 
CTCAGGAAGG AAAGAATACT GGAAATCAGC AAATCCCGGC GAAAAGCTCT AGAAGAATCT 
CTTCAGTTGC AAAAGTTCCT GGAAGCAAGT TATGAGATTT CCTCATGGCT GAATGAGAAG 
AACTCAGTGG CTGTTGACGA GAGTTGGAAA GATCCTATAA ATCTGCAGGC TAAGCTACTG 
AAACACCAGA GCTTTGAAGC AGAGATTCTG GCCAACCGCA ACAGAGTGCA GGCACACATT 
AAAGATGGTG ATAACATGCT GGCTTTCAGT CATCCTGCTA AAAGCAAAAT AAAACCACGA 
ATAAAGGATG TCGATGACAA CTGGGACCAG CTTTTAAGCA ACTGCAAGCA TAAGAAGACA 
CGTCTACAGC AAGCGTATCA GGCCTTACAG TTCCAGCGTT CATTAGGTGA CATTGAGGAG 
TGGCTTGGGT CGGTGGAGGT GGAAGTGGCG AATGACGACT ATGGAGGTGA TCTGGCCTCT 
GTTAGCAGGC TGCTTAAGGC TCTACAGGGT CTGGAGGAAA TGGTAGACGC GCACATGGAG 
AAAGTCCAGG GGCTAGTGGA CACTGCCAAA GACTTCAGCT CTCAGGGCAA CTTCCTCGCA 
GATAATATAC AGCAGAGGGT TTGGGAGGTG GTCAACAGGT ATAATGGCCT GGCTGAGCCG 
ATGCAGACCC GCAGGGAGAC TCTGGAGTCG TGGCAGCTGC TGTTCCAGTT CTACAGGGAC 
TTAGAGGATG AGCTGCTGTG GATTGAGGAC AAGCTGCAGG CCACGGCCAC CAAAGACTGG 
GGCACCTCCC TACAGAGCAT ACAATCTATA GTCAAAAAAC ATCTGGTTGT GATGCAGGAA 
ATTGAGAACC GAACTCCTCT AATAATGGCT GTACAGGAGG CAGGACAGAA TTTGGTGCGA 
GGACGACACT TTGCATCACG TGAGATCAGT GAAAAACTTT CAGGGTTAAA GAAGAACTTT 
GACGCCCTGA AGAAGGAGTC AGCGAATAAA GATCGCCTGC TTCAACAAGC ACTGAAAATC 
CAGAGCTTTC TCTCAGAGGT TTCCCAGTTG GAGCAGTGGA TGGAGGAACA GAAGCCTGTT 
TTAGAGTCCC GCGACTATGG AAAGAATGAG GAGGCCACTG AAGCTTTTCT CAGGAAGCTG 
GACACAGTTG ACCTGGAGTT GGAAAGCCAG CGTGGAAAAG TAGAGGCTCT CCTGAAAACC 
GGCACAGATT TAGAGAAGTC TCAGCATCCC AACAGCCATT TAGTGAGTAA GAGCCTTGAA 
GATATGCAGG GTGAGTTTGA GACACTACTG CAACTGTCTG GTGAACGTCG CACAGAGCTG 
GAGAATCAGT ACAATTTCTA CATATTTGAG AGAGATGCCA GAGACCTACA GACCTGGCTC 
CTTTCTCGGA AGACCATAGC AGAATCTGAT GATTATGGAA AAGATCTGGA AGGAGTGGAA 
GCACTGCAAA AGAAGTTTGA GGATTTTTCA GAAGAGGTTG GGACTCTGGG CCAGAGTAAG 
CTGAGCACTG TTCAAAAGCT AAAGCAAAGT GTGCAGTCAG CAGATGCACA ACAAAAAGAG 
AAAGATCTAC TCAAGCTCTG GGAGGATTTA AACAAGGCTA TGAAAGCAAG AGCAGAGAAC 
CTGCATTCAG CTCGAGAAGT TCACCAGTTT GAACATGACC TGGATGAGCT TCGGATCTGG 
ATGAATGAGA AGGAAGTTGC TCTGGACTCT GAGGAGCTGA AGAATGACCT GCTTAGTGTC 
CAAGCTCTCA TCCGTCAACA TGAAGGACTG GAGAGAGACC TTGCAGTGAT AGAAGAAGAT 
GTGTCTCGTC GAAAAGAGGA GGGAAAAGCT TTGGTCCGCA GGTCACCTCG AGTAAGGGAC 
AGTTTGAGCA AGAGACTCCA GGAAGTGGAG GAGATCTGGA GAAACCTGCT GGAGAAAGCC 
AACCAGCGCA GACAAAGACT CCAACAGGCA GAGGCCGTTC AAAGATATCT GATTGAATGG 
AGAGAGATGA TGGGCTGGCT GAAAGAGACA CTGTCTCTGA TGACAGGGGA GGGATTCGGA 
GGCAAGGTTA AAGACCTGGA ACAGATGATT AAAAAACATG ATGAGTACCG CACACAGATT 
GACAGACAAC TGGACAAATC GGAAATGGTT AAAAATGATG GGAGACGTCT GATGGAGGAG 
GGGAACTCAT GGAGTCATGA GCTTGAGGAG CGTCTAGCCG AGCTCAAGGA TCTGGAGGTG 
CGGGTGCTGG AGGCCTGGAC TGAAAGGAGA GCTCAGTATC AGGAAGACAT AGAGCTCCAG 
CAACTGCAGA GGGAACTGGA GCAGGCCGAG CACTGGCTCA ACACTTATGA GAGCGCGCTC 
AGAGCAGAGG ACTATGGGGA ATCAGTTTCA GATGTTCTGG ATTTGATGAA AAAGCAGGAA 
GATCTGGAGG CCATGATTCA AGCACAGAGT GAGAGATTCA ATGCCCTTCA AGCCAGAAGA 
ACACAGAGGG AAAATAAGAT GAAAGAAATT CAAAGAGGTG ACAGTACAGA CAGCAGCAGG 
GACAAGCCTG TTCGTGTAAC ATCCCTAAAG CGAAAGCCAT CTGAGCGGCC AGCATTGCCC 
CCTCGTCCCT TGTTGACCAG TCCAGTCCTA AAGAACAGCG ATTCACCCAC ACCTTCCCGA 
GTCCCTACCC CCAGGTCTGC CTTGTGGGAA AGCAGAAGTG ATAAAACTGA TTCTCCTGTG 
GTGTCACGAG TGGCAGCAGG ACTCAGAAGA AAGAGCAGCA GTGACAGTGA TAGTGTAACA 
TCACCTGACA AACCATCCAC TTCTGGTCAG GATAGCCCTG ACTCCTCCTC CACTGCGGTT 
TACAGACCTC ATTATCCACA CAATGCTTCT AAGGTTAAAC ATACCACATC TTCACAAGAT 
GAAGTCGATA CTCTAAGAGA GACTGAATCC TTTTTGAAAA CCATTGATAA ACCAAAAATC 
ACTCCTAGGC GTCAGAAACC ATCAACTCCT GATAAAGAGT TGTGGGGTCC TCCTACATCC 
AAACCACCTG AGCCACCCAT TAAACCTTCA CAATCATTGT CCAGATCTCC TCAGTCACTG 
TCCAGATCTC CTGAACCACT GTCCAGATCT CCAGAGCCAT TGCCTAGATC TCCTGAGCCA 
CTGTCCAGAT CTTCCCAGTC ACAGCCCAGA TCTCCTGAAC CACTATCCAA ATCGCCTGAA 
CCACTATCCA GATCTCCTGA GGCACTGTCC AGATCTCCCG AGCCACTGCC CAGATCTCCT 
CAGTCACTGT CTAGATCTCC TGAGCCACTG TCCAGATCTC CTGAGTCACC GTATATTCCT 
CCACTCACAA AACCTCCATC CACAAACCCT CCTGATCCAC CATCCAAGGC CACAGAATTG 
GTGGACAACC AAGACTCAAA AGATAGGAAC CTTCCTCAAT CCACTCCACC ATCAGTCCCT 
CAGTCATCAC CTCAATCTTT CGTTTCCAAC CCAGAACCAC AAGCGTCCTC TTCTGTGCAA 
TCCTCAATAG TCTTTCCAGC TGCTTCCACC ATGATCAGAG CAGAAGATGC CAAAATGGAG 
GGTACGTTGG AGATTAAACT GAAGCAAGGA GGAAATACAG GCTTGGATCA CTGGGAAACG 
GTCTATGCAC TACTGGAAGA GGAGACACTC AATCTGTTTA AAGACCAAGA TGCCGCCACT 
GAGAACTGTA CACGCTGGCC ACCGATCAAT TTGGCTGGAG CTATTTGTAA AGACAACCCT 
TTTTACAGGA GAAAGGAGCA CACGTTCAAA CTCATGCTGA GTGATGGCAG TCACTATCTG 
TTTGCTGCAT CATCTCAAGG AGAACAGCAG AAGTGGCTGA AGGAGCTCCA GGAATGTATT 
AATCCAACTG TGTCTTCAGA CAATCCTGGA ATGAAGAGAG AATCAAGCAG TGAAAGCGTA 
GAGAAGAGCG AGTCTGTGCC AGTCGACACA TCTGACACAC AAGACCAGGA GAAGGAAGAC 
TCCACCGAGA GCTGTGAGAC AGAGAGAGAA CCACCACCAA AACCTCCACA CACGTACTAC 
AACAAACACA AATACCCTGA CGGAGGAGAG AGCCAAGACT CAGTGAGTGT GTCTCAAAAT 
ATAAAAGGGC TACAGCCTCC CACATTCGCT CCTCCTCCTC CTCCAACACA AAACCAGACA 
GAGAATGAAG CCAAGGCCAA AAACAGGAGC GGATTTATGA AGTTTTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn5 ( XM_021467432.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323107.1 Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X2, mRNA.

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CloneID	ODa45808
Clone ID Related Accession (Same CDS sequence)	XM_021467432.1
Accession Version	XM_021467432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	spectrin beta chain, non-erythrocytic 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGAC TCTGTGTTCA TTTTATGATT GTGGACCTTC TGAAAGAGCT GGATGATATG 
AAGGGTCTGT ATGAGAGTAT GGTGTCTGAC TTGCTACGCT GGATCAAGAC CAAGGTGGTG 
GAGCTCAATG ACCGTTCCTT CCCCAACTCC CTGCGAGAAA TGCAGCATCT GGTAGCTGCC 
TTTAAGAAAT ACCGTACTAT TGAGAAGCCT CCCAAATACC AGGAACGGGG GGCCATTGAG 
GCACACCTCT TCGACTTACG AACCAAGCTG AGGGCAAACA ACCAGAGGGA CTACATTCCT 
CCGGAAGGCA AGACCTTGGG GGATATTGAA AGGAACTGGA CCTTGCTAGA GCGAGCAGAA 
CACCAGCGAG AACGGGTTCT CCAGAGTGCA CTGATGAGGC TGGAGCAGCT GGAGCAGTTG 
GCCCAGAAGT TTGGCCGTAA AGCGACGCTG AGGGAGAGCT ACCTGGAGGA CACTCTGCAA 
CTGATTCAAC AACAAGACCT GGGAGGACTG CAGAGGCTGG AGGAGGCTGA GATAGCAGCT 
CGCAGACTGG AGGCCCTGGG AGCTGATGTT TTGGCACATG AACCACGTTT CCGTGCTTTA 
AGTGAAATGG CTGCTGTCAT CGAGAGGGAT AACTACCACA GCAAAGTACA AGTGATTCGC 
AGAAATGTGG ACATCATGAA CCGCTGGAAG GATTTGAAGC AGGTTCTGCA GGAGCAGAGA 
GCACATGTTG GAGATGCGGC AAATACATTT GCTGTCCTTA GAGACATTGA GTTAATATCA 
ATGGACCTCA AGGAATTACA GGCTCAGGCA GATTCCTCGG ACATGGGAAA ACAGCTGCCA 
GATGTGGTAG CCCTACTGCA AAAGCAGGAC TTGATTGACA CTCAGATCAT CTCACTTGGT 
GAAACTCTGA ATGCCATCAG TAGTAGCAGT GCCTTAAAAG TTAAACACAA AGGTGCCACA 
AAAGTCCAGA GAGAACTTAA AGATCTAAAT AGCCATTACA ACTCCCTACT GGGTCTCAGC 
AAGAACAGGA GAAAAGCTCT TGAGGGACAG CTGAAGCTCT TTGAGTTTTT CCATGATTGT 
GAAGAAGTAG AAGCATGGAT CTATGAGAAA TGGGTCCTCT TACAGGCAGC ATCACTAGGG 
AGAGACCTCA GTCAAATTCA GCAGGCCATT CAGAACCACA AGGCTTTGGA GGCAGAGATT 
CAGTCACAGG AATCTCTGTG CTCTGGGGTT GTATTTAGAG GACAAGAGCT TTGTAGAGGG 
AGACACCCTA ATGAGAATGA CATCCAAAAG TGGATTCGGA CTCTTCAGAA ACAGTGGCAG 
CAACTTAAAG AACAAGTCAC CATCCGAAAG AACCGGCTGC ATGCTGCCAA TGTCATCAAG 
CAGTATTTTG CAGACATTAC TGAAGCCAGC TCTTGGCTTG ATGACCACAA ACCACTTCTC 
ATTGACGAGG ATTATGGAAA GGATGAGGCC AGCTCTACTG CTCTGCTCCA GCGTCACCAA 
CGAGTGGAGA AAGAAATGGA AGCATATTCC TCAGAAGTCA AACGACTGGG TGAGCAGGCC 
AAGAGCGCTG CCCAACTTGC TCCTCTTACA ACTGAAACCC AGCAAACGAG AATGGTCCAC 
TACAGTGATT CATCAGATGG CGAAGATGAG AAGGAGAAAA AAGCTGACAA AGGAGGCAAA 
GCAGTCAGCA AGATTTTACA ACAAGAACAA GCAATGATTA GGTTTAAATA CAGAGGCCCT 
AAACTAATCT GGGACAGAGG AGAAATATTG ACAATTATCA GCAGGGAAAA GGATGATAAG 
TTGCTTCTTC GGGACAGCAG AGGAAATGAG CAACTAGTCT CTTCCACATA CATCAGGGAG 
CTGCCAGTTT CTCAGGAAGC ACCACCTGAA ACTACAAACG GAGTAGCTGA GAGTCCAAAA 
AGAAAAGTGA GCCGCCCTCG CCGCACTCGA TCCATGCGGC GTGGCACAGC TGAAATATCT 
ACCTCAACCT TACCAGACCC ACATTTCCAA AAAGACACAA TAGAGAGCAC ACAAAACAGT 
CTGGAAAAGG ATTTCAACCG CCTTTACAAT TTAGCACAGT CAAAAAATAA GATCCTGGAG 
GACACTGCAC GACTACACAG ATTCTACAAT GCTTGTGAGG AGTTTGAGTC CTGGATGGGA 
GACAAGGAGA ATGTCCTCAA CACTTTCAGC TCCAATTCCA ACAACTTAGA GGTTGTACAA 
GCCAAATATG AGAATTTTGT AACTGAACTA GCCAGTGGTA GAAAGCAGCT TGATGATATT 
ACCAAGTTTG GAGATGAGCT ATTGAAACAA CAGCACAGCA GGAAAAAAGA CATTCAGCAA 
AGACTTGGAA TTGTTACTCG CAGATGGGAA CGTATTCAGA AACTAAAGGA TGAGATGGCT 
CATGAGCTTC TGAGTTCTGC TGATGTGAAG TCCTTCCTGC AGACCTGCCA GGAAGTCCAA 
GCTCAGCTCC AGCACAGACT CGCCCAGCTT GACACACCTG ATGTGGGGAG CTCACCATCA 
GCCTTACAAG CTGAGGAACA CAGACAAGCC CAAGCTGAAA GGGACATAGA AGCCCTTGAA 
AGGAAAATAG ACTATCTTAA AAGTGTGGCC AAGATGAAAA AGGACTGTAG TCCTGCAGAA 
AGTGCTGCTA TCATGGAAGA AGTACATGCT CTGGAAGACT TGTTGCGGCA GTTAAAGGCT 
CAAGCCATTC AAAGACAACG AATGCTTGAA GAAGCCAGAC GCTTGCAACT CTTTCAAAAA 
GAGACCAGAG ACCTGTTGAT GTGGGCTGAG GCAACACAGG AGCATCTTCT GGAAGAGGAG 
ACAGGATCTG ATGTAGCATC GGCTCAAGCC TTGTTGAAGG AGAACCTTGA TCTCAAGCAG 
GAGATTGAAC TGCAGAAAGC AAAATTAAAA GATATGGAGA GGCTGGGTCA GTCTCTTGAC 
GTGGCATCTG GAGAGAAAGG AGGTAAAGAT GTTCAGCAGA CACTTACAAA GTTGGATCAT 
GAGTGGAGCC AGCTGGACAA ACTGTGGTCT AGTCGCAACA GAAGGCTTGA ACAAGGACTG 
GAGTTTCAGA AACTGAATAT GGAAGCAGAT CGTATTGAGG CCACCATTTC TGGTCATGAA 
GCCAGACTTA AAGTCAGAGA CTTAGGGGAC TCTGTGGACA GTGTTCACAG TTTATTGAGC 
CGACAAGAAG AGCTTGAGGC TTTGCTTGAG GCTCTAGAGC GGTCTATTAT CCTCTTTCAA 
GACAAGTGCC AGGAGCTCAT CAGCAAGCGT AACTTTGCTG CTAAAGAAAT TCAACAGCGA 
TCAAAGGTCA TTCAAGATCA TTTCAAGAGG CTGAAAGAAA GTTGTAAATT AAGGAGGCTT 
GAATTACTTG CTTCGAAAAA GTACCAGGAA TTTTTAAGAG ATGCTGAGGA GATGCTGTTG 
TGGATGGATG AAAAGTTTGA GATCGCTGAG GATGAATCGT ACAGAGATCC AACCAACATC 
CTTCGTAAGC TTAAAAAACA TGAGGCAGCA GAAAAAGAGA TGCAGGCCAA TGCAGTTCGC 
ATAGACAGAC TAATTGAGAC TGGAGAGAAG ATGTTGGCAG ATGATCACTA CAACACCCAG 
AGCATTCAGA AAAAGACTCA AGAGGTACAC AAAAGGTGGA TTGAACTTCA GAGGAAGATG 
GCTGAAAGAG GGGACAAGCT GCGACAGGCT GGCCAACAGG AGCAACTCAT GGAGCTGCTG 
CAGGATGCTA AATTGAAGAT TGAGGCAATT GAGAGAATGC TACAAAATGC AATCAGGGGT 
CATGACCTCC GCTCCAGCAG ACAGCTACTA AAAGAGCACA AGCAGCTGGA AGAGGAAGCT 
CAAGAACTTG CTGAGAAAAT CAATTCTATT GTCACCCGTG CCAAAAACCT GGCCACCAAC 
CATTTTGACT CTCAGCGCAT TCTCAAGGAG ACTGACACTT ACCTGAAACT ATTTAAGTCT 
CTAGAGAAAC CTTTGGATCA TCGCAGGAGC CAGTTAGAGG CAGATGTGGA TCTATACAGC 
TTTTACCATG ATGTAGACCT AGAGCTAAAC TGGATATCAG AGCATCAGCC TGTAGCTGAT 
ACTACAAACT ATGAGAGATC ACTGGTTGGG GCAGTTAGCC TTCTTCAAAA ACATAAGGAC 
CTGCAGGCGG AGGTGAATGC CCACAGCAAG TACCTGCAGC GAGTACTGGA CAGAGGACGA 
GCCATGGCCC GATCCAATCA GTGGAATGGT CAGGAGGTGC AGCAGAGGTG TGAGCAATTG 
ATCGCAAAGT GGGAAGAGTT AGAGGAATCC TGCAAGAAAC GCTCCGGAGA ACTTAACAGG 
GCTGTTACAC GGGAACAGAT TCTGCTTGAC TGCACTGAGC TGGAAACCCT TGTGTCAGAA 
ACATCAGCTT TAGTCAGCAC TGATTATGGC AAGACTGAAT TAGCTACCCG CAGTCTAATA 
AAGCAACACG AGGGAGTGGA GGGGCAAATC GAGGTACTTG CTGCTCAAGT AGATGAACTC 
AAGTCCAATG TCAAGCAGGC TGTACGTGTG TGGGGTTTAG AGGAGTTGAA CAAGCCCTGC 
AACCACATTA AATCTAAGCT GAGTGAGCTT CAACACTCAG CGACAGTCAG AGAACAGAGG 
CTTCGAGAGA CTCTACACCT ACATGAATTT AAGCGTGAGA CTAGTGAACT GGAGGAATGG 
ATTGCTCAGC AGACACAGAT TGCAGCCTCT AATGATCTTG GGAGTGATTA TGAAAATGCA 
ATGCAACTGC GTGGGAGATT TGAGGTCTTC TTGAAGCAGC TGGAGGTGGG AAAGGAGAGG 
ATGCACACCT GTGAACAGTT GGCTGACAAA CTCATCAAAA GCAATCACCC TGAGAGTCGC 
TTCATCCGAG AAACACAGAA TCTGCTCAGA GAATCGTGGG ACGAGCTGCA GGATTTATCG 
AAAGATCGTA AAGAAAAGCT TCGTAAGTCT GAAAAATGTC ACAAATTCTA CAAAGACCTG 
ACCGATGCAC TGGGACAGAT CAAGGAGAGG CACAAAACCA TTCCTGAAGA CATAGCCAAA 
GATCTGAAAG GAGTGCAGTC ACAGCTACGC AAACATGAAG CTCTTGTGCA CGAGCTGGCT 
GGAACTGAGC AACAGCTGCA GGAGCTGTTG GATGGAACCG ATGCTATTTT GGGCATGTGC 
TCTCCTGAGC TGCGGGTGGG GTTGCAGGAA CTACAACAGG AGGTGGTGGA GAACTGGGAG 
CGGCTCCGCA TGCGGATGGA GCAGAGAGAA GAGGAGCTGC AATCGGCTAA AGACCGCTAC 
ATGTTCCTCA ACACGGCACA GGACTACTCC CTGTGGTGCT CTCAGGTGCT GAGCTGGATG 
AAGGCAGAGG AGTCCATTCG AGACGTTGCC ACCTGTGACC TGCAGTTGTT CCAGCACCAG 
CAGTTGTGGG CTGAGATTGT GGCCCGTGAG GAGACGTTTG CCCAGGCTGT GTCAATGGGT 
CAAGGCCTTC TGGAGAATGA CATACCTAAT GCTAGAGAGA TCCAGGAGAA ACTGAAAGCT 
CTGCAAGAAG AGAGAGAGAA GCTTTCCGAT AACTGGCAGC TCAGGCAGAA ATGGCTGGAG 
AACACATATC TGGAGCAGGT GTTTTACAGA GACACTGAGT ACATGGAGAA GATCACCAAC 
TCTCAAGAGA TCCAGTTGAA GAACAGTGAT TTAGGAACGA CAGTGGATGA TGTTGAAAGT 
TTGATTAAGC GCCATGAGGC CTTTGAAAAA CTTCTTCATT CTCAGGAAGA TAAGATGGTG 
GCTCTTCAGG AATCAGCAGA GCGTTTAAAG ACAGAAGGTT TAACACGAGA AAAAAACAGC 
AATATTAAGA ACAAACTCAA AGCTCTTCAG GAAAGGAGGG AACGGATTAA AGATCTGTCT 
GGCAAACGAA GAGAGGATCT TGAGGTCTCA AAGCTTCACT GTATTTTCAG CCGAGATGTG 
TCTGAGGCAG AGGAATGGAT CACAGATCGT ATGAACAAGA TACAAGATGA CTCCAAAATG 
GACATGAGCA ACCTTCAGAC TAAAATGAAG ATGCTGCAGA AGCATCAGGT GTTTGAAGCT 
GAGATTCTGG CCCATGGAAA TATCATTGAA TCTGTCCAGC AGGCTGGAAA TGAGCTGGTG 
ACCATGCATC ACCCAAAATC AAAGGAGATA CGCCAGACTG TTTCAGCGCT CATCTCTCAT 
TGGAAGGCTC TAAAGCAAGA GCTTGCGGTG CGTGGAAAGG TTCTAGAGGA TAACCGTGAT 
TTTCTTGAGT TCCTGCAGAA AGTGGAACAG GTTGAAGTTT GGATTAGGCA AAAGGAGGTG 
ATGATAAATG TTGGAGATGT CGGGGAGGAT TATGAACACG GTCAGCAGCT GCTGAAGAAG 
CTCAGTGAAT TCAGAGGGTC AGGCTCTGGA GATGTGACTG TTGACGATGC TCACATCAAG 
ACAATCAACA CTTTGGCCGC TCGACTTGAG AGACAGAACA GTGATGAGCT GGTGACTGTG 
AAAAAGCGTA GGCAGCAGCT CAATGAAAGA TGGAGGAATT TCCATGGCAA CCTGAGCAGC 
TATAAGAGGA AGCTGGAGGC AGCGTTGGAG GTCCACGCAT TGATCCGAGA ACTAGAGGAG 
GTCCGGGACA GAGCTGGAGA GAAGATGCTG TTGCTCCAGG GTCAGGGTTG CGGGTTGGAT 
GTTGAAAGTG TGGAGAACCT CATTCGCAGA CATGAAGAGA TGGAGAGGGA GGCCAGGGTC 
ATCCAGGAGA GAGGCGCTGC CCTGGAAAAG GAGACTAAAG ATCGGTTGAA GAGACACTGC 
GATCTGTCTG ACAAACTTAG CAAGAAGCAG GAAGAGGTCA AAATTGCTTT AGTCAAGCTG 
GACAAGGAAA TCAAACGAAG GAAGGAGCGA TTGCAGGAGT CTCATCAGCT CCAGCTGTTT 
AAAGCGAATC AGCGTCTGCT TTTGGACTGG ACCCTCAAGC AAAGTGATGA GATGGTGAAG 
AAAGGCCTGC CTAAGAATAA AACCGAAGCT GAGAGTTTCA TATTAGAACA TCACGACTGG 
AAGGCTGAAA TTGATGCTCG TGGCGATCGG ATAGATTCAG TTAAGAGTTT CGGACAGAAT 
CTTGTGAAGT CTGGACACAG TGATGCAGCA GAGATTAAAG AAGCCCTACG CAAGCTGGAT 
GATGCCAAGA CAATGCTTAC CCGAGCTTGG GAGGACCGAA GAAAAACTCT GGATCAAGCC 
CTGAAGCTCC AGATATTCCT GGGCTATGCT GACCAGACTG AGAGCTGGAT GAGTAACAGG 
GAGGCTTTCT TGATCAATGA AGATCTAGGG GGTTCATTGT CGGAGGTGGA GGCTCTACAG 
AGGAAGCTGG CTCTGTTTGA GAACTCTCTA GAAGCTCAGA TGGAGCAGGT TTCAGAGGTT 
GACAGATACG CTCAGCAGCT CATACAGGCA AACCACTACG ACTCTGACAA CATCAAGAAG 
AGGACAAAAG CTATTCTGCT CAGGAAGGAA AGAATACTGG AAATCAGCAA ATCCCGGCGA 
AAAGCTCTAG AAGAATCTCT TCAGTTGCAA AAGTTCCTGG AAGCAAGTTA TGAGATTTCC 
TCATGGCTGA ATGAGAAGAA CTCAGTGGCT GTTGACGAGA GTTGGAAAGA TCCTATAAAT 
CTGCAGGCTA AGCTACTGAA ACACCAGAGC TTTGAAGCAG AGATTCTGGC CAACCGCAAC 
AGAGTGCAGG CACACATTAA AGATGGTGAT AACATGCTGG CTTTCAGTCA TCCTGCTAAA 
AGCAAAATAA AACCACGAAT AAAGGATGTC GATGACAACT GGGACCAGCT TTTAAGCAAC 
TGCAAGCATA AGAAGACACG TCTACAGCAA GCGTATCAGG CCTTACAGTT CCAGCGTTCA 
TTAGGTGACA TTGAGGAGTG GCTTGGGTCG GTGGAGGTGG AAGTGGCGAA TGACGACTAT 
GGAGGTGATC TGGCCTCTGT TAGCAGGCTG CTTAAGGCTC TACAGGGTCT GGAGGAAATG 
GTAGACGCGC ACATGGAGAA AGTCCAGGGG CTAGTGGACA CTGCCAAAGA CTTCAGCTCT 
CAGGGCAACT TCCTCGCAGA TAATATACAG CAGAGGGTTT GGGAGGTGGT CAACAGGTAT 
AATGGCCTGG CTGAGCCGAT GCAGACCCGC AGGGAGACTC TGGAGTCGTG GCAGCTGCTG 
TTCCAGTTCT ACAGGGACTT AGAGGATGAG CTGCTGTGGA TTGAGGACAA GCTGCAGGCC 
ACGGCCACCA AAGACTGGGG CACCTCCCTA CAGAGCATAC AATCTATAGT CAAAAAACAT 
CTGGTTGTGA TGCAGGAAAT TGAGAACCGA ACTCCTCTAA TAATGGCTGT ACAGGAGGCA 
GGACAGAATT TGGTGCGAGG ACGACACTTT GCATCACGTG AGATCAGTGA AAAACTTTCA 
GGGTTAAAGA AGAACTTTGA CGCCCTGAAG AAGGAGTCAG CGAATAAAGA TCGCCTGCTT 
CAACAAGCAC TGAAAATCCA GAGCTTTCTC TCAGAGGTTT CCCAGTTGGA GCAGTGGATG 
GAGGAACAGA AGCCTGTTTT AGAGTCCCGC GACTATGGAA AGAATGAGGA GGCCACTGAA 
GCTTTTCTCA GGAAGCTGGA CACAGTTGAC CTGGAGTTGG AAAGCCAGCG TGGAAAAGTA 
GAGGCTCTCC TGAAAACCGG CACAGATTTA GAGAAGTCTC AGCATCCCAA CAGCCATTTA 
GTGAGTAAGA GCCTTGAAGA TATGCAGGGT GAGTTTGAGA CACTACTGCA ACTGTCTGGT 
GAACGTCGCA CAGAGCTGGA GAATCAGTAC AATTTCTACA TATTTGAGAG AGATGCCAGA 
GACCTACAGA CCTGGCTCCT TTCTCGGAAG ACCATAGCAG AATCTGATGA TTATGGAAAA 
GATCTGGAAG GAGTGGAAGC ACTGCAAAAG AAGTTTGAGG ATTTTTCAGA AGAGGTTGGG 
ACTCTGGGCC AGAGTAAGCT GAGCACTGTT CAAAAGCTAA AGCAAAGTGT GCAGTCAGCA 
GATGCACAAC AAAAAGAGAA AGATCTACTC AAGCTCTGGG AGGATTTAAA CAAGGCTATG 
AAAGCAAGAG CAGAGAACCT GCATTCAGCT CGAGAAGTTC ACCAGTTTGA ACATGACCTG 
GATGAGCTTC GGATCTGGAT GAATGAGAAG GAAGTTGCTC TGGACTCTGA GGAGCTGAAG 
AATGACCTGC TTAGTGTCCA AGCTCTCATC CGTCAACATG AAGGACTGGA GAGAGACCTT 
GCAGTGATAG AAGAAGATGT GTCTCGTCGA AAAGAGGAGG GAAAAGCTTT GGTCCGCAGG 
TCACCTCGAG TAAGGGACAG TTTGAGCAAG AGACTCCAGG AAGTGGAGGA GATCTGGAGA 
AACCTGCTGG AGAAAGCCAA CCAGCGCAGA CAAAGACTCC AACAGGCAGA GGCCGTTCAA 
AGATATCTGA TTGAATGGAG AGAGATGATG GGCTGGCTGA AAGAGACACT GTCTCTGATG 
ACAGGGGAGG GATTCGGAGG CAAGGTTAAA GACCTGGAAC AGATGATTAA AAAACATGAT 
GAGTACCGCA CACAGATTGA CAGACAACTG GACAAATCGG AAATGGTTAA AAATGATGGG 
AGACGTCTGA TGGAGGAGGG GAACTCATGG AGTCATGAGC TTGAGGAGCG TCTAGCCGAG 
CTCAAGGATC TGGAGGTGCG GGTGCTGGAG GCCTGGACTG AAAGGAGAGC TCAGTATCAG 
GAAGACATAG AGCTCCAGCA ACTGCAGAGG GAACTGGAGC AGGCCGAGCA CTGGCTCAAC 
ACTTATGAGA GCGCGCTCAG AGCAGAGGAC TATGGGGAAT CAGTTTCAGA TGTTCTGGAT 
TTGATGAAAA AGCAGGAAGA TCTGGAGGCC ATGATTCAAG CACAGAGTGA GAGATTCAAT 
GCCCTTCAAG CCAGAAGAAC ACAGAGGGAA AATAAGATGA AAGAAATTCA AAGAGGTGAC 
AGTACAGACA GCAGCAGGGA CAAGCCTGTT CGTGTAACAT CCCTAAAGCG AAAGCCATCT 
GAGCGGCCAG CATTGCCCCC TCGTCCCTTG TTGACCAGTC CAGTCCTAAA GAACAGCGAT 
TCACCCACAC CTTCCCGAGT CCCTACCCCC AGGTCTGCCT TGTGGGAAAG CAGAAGTGAT 
AAAACTGATT CTCCTGTGGT GTCACGAGTG GCAGCAGGAC TCAGAAGAAA GAGCAGCAGT 
GACAGTGATA GTGTAACATC ACCTGACAAA CCATCCACTT CTGGTCAGGA TAGCCCTGAC 
TCCTCCTCCA CTGCGGTTTA CAGACCTCAT TATCCACACA ATGCTTCTAA GGTTAAACAT 
ACCACATCTT CACAAGATGA AGTCGATACT CTAAGAGAGA CTGAATCCTT TTTGAAAACC 
ATTGATAAAC CAAAAATCAC TCCTAGGCGT CAGAAACCAT CAACTCCTGA TAAAGAGTTG 
TGGGGTCCTC CTACATCCAA ACCACCTGAG CCACCCATTA AACCTTCACA ATCATTGTCC 
AGATCTCCTC AGTCACTGTC CAGATCTCCT GAACCACTGT CCAGATCTCC AGAGCCATTG 
CCTAGATCTC CTGAGCCACT GTCCAGATCT TCCCAGTCAC AGCCCAGATC TCCTGAACCA 
CTATCCAAAT CGCCTGAACC ACTATCCAGA TCTCCTGAGG CACTGTCCAG ATCTCCCGAG 
CCACTGCCCA GATCTCCTCA GTCACTGTCT AGATCTCCTG AGCCACTGTC CAGATCTCCT 
GAGTCACCGT ATATTCCTCC ACTCACAAAA CCTCCATCCA CAAACCCTCC TGATCCACCA 
TCCAAGGCCA CAGAATTGGT GGACAACCAA GACTCAAAAG ATAGGAACCT TCCTCAATCC 
ACTCCACCAT CAGTCCCTCA GTCATCACCT CAATCTTTCG TTTCCAACCC AGAACCACAA 
GCGTCCTCTT CTGTGCAATC CTCAATAGTC TTTCCAGCTG CTTCCACCAT GATCAGAGCA 
GAAGATGCCA AAATGGAGGG TACGTTGGAG ATTAAACTGA AGCAAGGAGG AAATACAGGC 
TTGGATCACT GGGAAACGGT CTATGCACTA CTGGAAGAGG AGACACTCAA TCTGTTTAAA 
GACCAAGATG CCGCCACTGA GAACTGTACA CGCTGGCCAC CGATCAATTT GGCTGGAGCT 
ATTTGTAAAG ACAACCCTTT TTACAGGAGA AAGGAGCACA CGTTCAAACT CATGCTGAGT 
GATGGCAGTC ACTATCTGTT TGCTGCATCA TCTCAAGGAG AACAGCAGAA GTGGCTGAAG 
GAGCTCCAGG AATGTATTAA TCCAACTGTG TCTTCAGACA ATCCTGGAAT GAAGAGAGAA 
TCAAGCAGTG AAAGCGTAGA GAAGAGCGAG TCTGTGCCAG TCGACACATC TGACACACAA 
GACCAGGAGA AGGAAGACTC CACCGAGAGC TGTGAGACAG AGAGAGAACC ACCACCAAAA 
CCTCCACACA CGTACTACAA CAAACACAAA TACCCTGACG GAGGAGAGAG CCAAGACTCA 
GTGAGTGTGT CTCAAAATAT AAAAGGGCTA CAGCCTCCCA CATTCGCTCC TCCTCCTCCT 
CCAACACAAA ACCAGACAGA GAATGAAGCC AAGGCCAAAA ACAGGAGCGG ATTTATGAAG 
TTTTTCAGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323107.1
CDS	466..12120
Translation	MSRLCVHFMIVDLLKELDDMKGLYESMVSDLLRWIKTKVVELNDRSFPNSLREMQHLVAAFKKYRTIEKPPKYQERGAIEAHLFDLRTKLRANNQRDYIPPEGKTLGDIERNWTLLERAEHQRERVLQSALMRLEQLEQLAQKFGRKATLRESYLEDTLQLIQQQDLGGLQRLEEAEIAARRLEALGADVLAHEPRFRALSEMAAVIERDNYHSKVQVIRRNVDIMNRWKDLKQVLQEQRAHVGDAANTFAVLRDIELISMDLKELQAQADSSDMGKQLPDVVALLQKQDLIDTQIISLGETLNAISSSSALKVKHKGATKVQRELKDLNSHYNSLLGLSKNRRKALEGQLKLFEFFHDCEEVEAWIYEKWVLLQAASLGRDLSQIQQAIQNHKALEAEIQSQESLCSGVVFRGQELCRGRHPNENDIQKWIRTLQKQWQQLKEQVTIRKNRLHAANVIKQYFADITEASSWLDDHKPLLIDEDYGKDEASSTALLQRHQRVEKEMEAYSSEVKRLGEQAKSAAQLAPLTTETQQTRMVHYSDSSDGEDEKEKKADKGGKAVSKILQQEQAMIRFKYRGPKLIWDRGEILTIISREKDDKLLLRDSRGNEQLVSSTYIRELPVSQEAPPETTNGVAESPKRKVSRPRRTRSMRRGTAEISTSTLPDPHFQKDTIESTQNSLEKDFNRLYNLAQSKNKILEDTARLHRFYNACEEFESWMGDKENVLNTFSSNSNNLEVVQAKYENFVTELASGRKQLDDITKFGDELLKQQHSRKKDIQQRLGIVTRRWERIQKLKDEMAHELLSSADVKSFLQTCQEVQAQLQHRLAQLDTPDVGSSPSALQAEEHRQAQAERDIEALERKIDYLKSVAKMKKDCSPAESAAIMEEVHALEDLLRQLKAQAIQRQRMLEEARRLQLFQKETRDLLMWAEATQEHLLEEETGSDVASAQALLKENLDLKQEIELQKAKLKDMERLGQSLDVASGEKGGKDVQQTLTKLDHEWSQLDKLWSSRNRRLEQGLEFQKLNMEADRIEATISGHEARLKVRDLGDSVDSVHSLLSRQEELEALLEALERSIILFQDKCQELISKRNFAAKEIQQRSKVIQDHFKRLKESCKLRRLELLASKKYQEFLRDAEEMLLWMDEKFEIAEDESYRDPTNILRKLKKHEAAEKEMQANAVRIDRLIETGEKMLADDHYNTQSIQKKTQEVHKRWIELQRKMAERGDKLRQAGQQEQLMELLQDAKLKIEAIERMLQNAIRGHDLRSSRQLLKEHKQLEEEAQELAEKINSIVTRAKNLATNHFDSQRILKETDTYLKLFKSLEKPLDHRRSQLEADVDLYSFYHDVDLELNWISEHQPVADTTNYERSLVGAVSLLQKHKDLQAEVNAHSKYLQRVLDRGRAMARSNQWNGQEVQQRCEQLIAKWEELEESCKKRSGELNRAVTREQILLDCTELETLVSETSALVSTDYGKTELATRSLIKQHEGVEGQIEVLAAQVDELKSNVKQAVRVWGLEELNKPCNHIKSKLSELQHSATVREQRLRETLHLHEFKRETSELEEWIAQQTQIAASNDLGSDYENAMQLRGRFEVFLKQLEVGKERMHTCEQLADKLIKSNHPESRFIRETQNLLRESWDELQDLSKDRKEKLRKSEKCHKFYKDLTDALGQIKERHKTIPEDIAKDLKGVQSQLRKHEALVHELAGTEQQLQELLDGTDAILGMCSPELRVGLQELQQEVVENWERLRMRMEQREEELQSAKDRYMFLNTAQDYSLWCSQVLSWMKAEESIRDVATCDLQLFQHQQLWAEIVAREETFAQAVSMGQGLLENDIPNAREIQEKLKALQEEREKLSDNWQLRQKWLENTYLEQVFYRDTEYMEKITNSQEIQLKNSDLGTTVDDVESLIKRHEAFEKLLHSQEDKMVALQESAERLKTEGLTREKNSNIKNKLKALQERRERIKDLSGKRREDLEVSKLHCIFSRDVSEAEEWITDRMNKIQDDSKMDMSNLQTKMKMLQKHQVFEAEILAHGNIIESVQQAGNELVTMHHPKSKEIRQTVSALISHWKALKQELAVRGKVLEDNRDFLEFLQKVEQVEVWIRQKEVMINVGDVGEDYEHGQQLLKKLSEFRGSGSGDVTVDDAHIKTINTLAARLERQNSDELVTVKKRRQQLNERWRNFHGNLSSYKRKLEAALEVHALIRELEEVRDRAGEKMLLLQGQGCGLDVESVENLIRRHEEMEREARVIQERGAALEKETKDRLKRHCDLSDKLSKKQEEVKIALVKLDKEIKRRKERLQESHQLQLFKANQRLLLDWTLKQSDEMVKKGLPKNKTEAESFILEHHDWKAEIDARGDRIDSVKSFGQNLVKSGHSDAAEIKEALRKLDDAKTMLTRAWEDRRKTLDQALKLQIFLGYADQTESWMSNREAFLINEDLGGSLSEVEALQRKLALFENSLEAQMEQVSEVDRYAQQLIQANHYDSDNIKKRTKAILLRKERILEISKSRRKALEESLQLQKFLEASYEISSWLNEKNSVAVDESWKDPINLQAKLLKHQSFEAEILANRNRVQAHIKDGDNMLAFSHPAKSKIKPRIKDVDDNWDQLLSNCKHKKTRLQQAYQALQFQRSLGDIEEWLGSVEVEVANDDYGGDLASVSRLLKALQGLEEMVDAHMEKVQGLVDTAKDFSSQGNFLADNIQQRVWEVVNRYNGLAEPMQTRRETLESWQLLFQFYRDLEDELLWIEDKLQATATKDWGTSLQSIQSIVKKHLVVMQEIENRTPLIMAVQEAGQNLVRGRHFASREISEKLSGLKKNFDALKKESANKDRLLQQALKIQSFLSEVSQLEQWMEEQKPVLESRDYGKNEEATEAFLRKLDTVDLELESQRGKVEALLKTGTDLEKSQHPNSHLVSKSLEDMQGEFETLLQLSGERRTELENQYNFYIFERDARDLQTWLLSRKTIAESDDYGKDLEGVEALQKKFEDFSEEVGTLGQSKLSTVQKLKQSVQSADAQQKEKDLLKLWEDLNKAMKARAENLHSAREVHQFEHDLDELRIWMNEKEVALDSEELKNDLLSVQALIRQHEGLERDLAVIEEDVSRRKEEGKALVRRSPRVRDSLSKRLQEVEEIWRNLLEKANQRRQRLQQAEAVQRYLIEWREMMGWLKETLSLMTGEGFGGKVKDLEQMIKKHDEYRTQIDRQLDKSEMVKNDGRRLMEEGNSWSHELEERLAELKDLEVRVLEAWTERRAQYQEDIELQQLQRELEQAEHWLNTYESALRAEDYGESVSDVLDLMKKQEDLEAMIQAQSERFNALQARRTQRENKMKEIQRGDSTDSSRDKPVRVTSLKRKPSERPALPPRPLLTSPVLKNSDSPTPSRVPTPRSALWESRSDKTDSPVVSRVAAGLRRKSSSDSDSVTSPDKPSTSGQDSPDSSSTAVYRPHYPHNASKVKHTTSSQDEVDTLRETESFLKTIDKPKITPRRQKPSTPDKELWGPPTSKPPEPPIKPSQSLSRSPQSLSRSPEPLSRSPEPLPRSPEPLSRSSQSQPRSPEPLSKSPEPLSRSPEALSRSPEPLPRSPQSLSRSPEPLSRSPESPYIPPLTKPPSTNPPDPPSKATELVDNQDSKDRNLPQSTPPSVPQSSPQSFVSNPEPQASSSVQSSIVFPAASTMIRAEDAKMEGTLEIKLKQGGNTGLDHWETVYALLEEETLNLFKDQDAATENCTRWPPINLAGAICKDNPFYRRKEHTFKLMLSDGSHYLFAASSQGEQQKWLKELQECINPTVSSDNPGMKRESSSESVEKSESVPVDTSDTQDQEKEDSTESCETEREPPPKPPHTYYNKHKYPDGGESQDSVSVSQNIKGLQPPTFAPPPPPTQNQTENEAKAKNRSGFMKFFRK

Target ORF information:

RefSeq Version	XM_021467432.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467432.1

ORF Insert Sequence:

ATGTCAAGAC TCTGTGTTCA TTTTATGATT GTGGACCTTC TGAAAGAGCT GGATGATATG 
AAGGGTCTGT ATGAGAGTAT GGTGTCTGAC TTGCTACGCT GGATCAAGAC CAAGGTGGTG 
GAGCTCAATG ACCGTTCCTT CCCCAACTCC CTGCGAGAAA TGCAGCATCT GGTAGCTGCC 
TTTAAGAAAT ACCGTACTAT TGAGAAGCCT CCCAAATACC AGGAACGGGG GGCCATTGAG 
GCACACCTCT TCGACTTACG AACCAAGCTG AGGGCAAACA ACCAGAGGGA CTACATTCCT 
CCGGAAGGCA AGACCTTGGG GGATATTGAA AGGAACTGGA CCTTGCTAGA GCGAGCAGAA 
CACCAGCGAG AACGGGTTCT CCAGAGTGCA CTGATGAGGC TGGAGCAGCT GGAGCAGTTG 
GCCCAGAAGT TTGGCCGTAA AGCGACGCTG AGGGAGAGCT ACCTGGAGGA CACTCTGCAA 
CTGATTCAAC AACAAGACCT GGGAGGACTG CAGAGGCTGG AGGAGGCTGA GATAGCAGCT 
CGCAGACTGG AGGCCCTGGG AGCTGATGTT TTGGCACATG AACCACGTTT CCGTGCTTTA 
AGTGAAATGG CTGCTGTCAT CGAGAGGGAT AACTACCACA GCAAAGTACA AGTGATTCGC 
AGAAATGTGG ACATCATGAA CCGCTGGAAG GATTTGAAGC AGGTTCTGCA GGAGCAGAGA 
GCACATGTTG GAGATGCGGC AAATACATTT GCTGTCCTTA GAGACATTGA GTTAATATCA 
ATGGACCTCA AGGAATTACA GGCTCAGGCA GATTCCTCGG ACATGGGAAA ACAGCTGCCA 
GATGTGGTAG CCCTACTGCA AAAGCAGGAC TTGATTGACA CTCAGATCAT CTCACTTGGT 
GAAACTCTGA ATGCCATCAG TAGTAGCAGT GCCTTAAAAG TTAAACACAA AGGTGCCACA 
AAAGTCCAGA GAGAACTTAA AGATCTAAAT AGCCATTACA ACTCCCTACT GGGTCTCAGC 
AAGAACAGGA GAAAAGCTCT TGAGGGACAG CTGAAGCTCT TTGAGTTTTT CCATGATTGT 
GAAGAAGTAG AAGCATGGAT CTATGAGAAA TGGGTCCTCT TACAGGCAGC ATCACTAGGG 
AGAGACCTCA GTCAAATTCA GCAGGCCATT CAGAACCACA AGGCTTTGGA GGCAGAGATT 
CAGTCACAGG AATCTCTGTG CTCTGGGGTT GTATTTAGAG GACAAGAGCT TTGTAGAGGG 
AGACACCCTA ATGAGAATGA CATCCAAAAG TGGATTCGGA CTCTTCAGAA ACAGTGGCAG 
CAACTTAAAG AACAAGTCAC CATCCGAAAG AACCGGCTGC ATGCTGCCAA TGTCATCAAG 
CAGTATTTTG CAGACATTAC TGAAGCCAGC TCTTGGCTTG ATGACCACAA ACCACTTCTC 
ATTGACGAGG ATTATGGAAA GGATGAGGCC AGCTCTACTG CTCTGCTCCA GCGTCACCAA 
CGAGTGGAGA AAGAAATGGA AGCATATTCC TCAGAAGTCA AACGACTGGG TGAGCAGGCC 
AAGAGCGCTG CCCAACTTGC TCCTCTTACA ACTGAAACCC AGCAAACGAG AATGGTCCAC 
TACAGTGATT CATCAGATGG CGAAGATGAG AAGGAGAAAA AAGCTGACAA AGGAGGCAAA 
GCAGTCAGCA AGATTTTACA ACAAGAACAA GCAATGATTA GGTTTAAATA CAGAGGCCCT 
AAACTAATCT GGGACAGAGG AGAAATATTG ACAATTATCA GCAGGGAAAA GGATGATAAG 
TTGCTTCTTC GGGACAGCAG AGGAAATGAG CAACTAGTCT CTTCCACATA CATCAGGGAG 
CTGCCAGTTT CTCAGGAAGC ACCACCTGAA ACTACAAACG GAGTAGCTGA GAGTCCAAAA 
AGAAAAGTGA GCCGCCCTCG CCGCACTCGA TCCATGCGGC GTGGCACAGC TGAAATATCT 
ACCTCAACCT TACCAGACCC ACATTTCCAA AAAGACACAA TAGAGAGCAC ACAAAACAGT 
CTGGAAAAGG ATTTCAACCG CCTTTACAAT TTAGCACAGT CAAAAAATAA GATCCTGGAG 
GACACTGCAC GACTACACAG ATTCTACAAT GCTTGTGAGG AGTTTGAGTC CTGGATGGGA 
GACAAGGAGA ATGTCCTCAA CACTTTCAGC TCCAATTCCA ACAACTTAGA GGTTGTACAA 
GCCAAATATG AGAATTTTGT AACTGAACTA GCCAGTGGTA GAAAGCAGCT TGATGATATT 
ACCAAGTTTG GAGATGAGCT ATTGAAACAA CAGCACAGCA GGAAAAAAGA CATTCAGCAA 
AGACTTGGAA TTGTTACTCG CAGATGGGAA CGTATTCAGA AACTAAAGGA TGAGATGGCT 
CATGAGCTTC TGAGTTCTGC TGATGTGAAG TCCTTCCTGC AGACCTGCCA GGAAGTCCAA 
GCTCAGCTCC AGCACAGACT CGCCCAGCTT GACACACCTG ATGTGGGGAG CTCACCATCA 
GCCTTACAAG CTGAGGAACA CAGACAAGCC CAAGCTGAAA GGGACATAGA AGCCCTTGAA 
AGGAAAATAG ACTATCTTAA AAGTGTGGCC AAGATGAAAA AGGACTGTAG TCCTGCAGAA 
AGTGCTGCTA TCATGGAAGA AGTACATGCT CTGGAAGACT TGTTGCGGCA GTTAAAGGCT 
CAAGCCATTC AAAGACAACG AATGCTTGAA GAAGCCAGAC GCTTGCAACT CTTTCAAAAA 
GAGACCAGAG ACCTGTTGAT GTGGGCTGAG GCAACACAGG AGCATCTTCT GGAAGAGGAG 
ACAGGATCTG ATGTAGCATC GGCTCAAGCC TTGTTGAAGG AGAACCTTGA TCTCAAGCAG 
GAGATTGAAC TGCAGAAAGC AAAATTAAAA GATATGGAGA GGCTGGGTCA GTCTCTTGAC 
GTGGCATCTG GAGAGAAAGG AGGTAAAGAT GTTCAGCAGA CACTTACAAA GTTGGATCAT 
GAGTGGAGCC AGCTGGACAA ACTGTGGTCT AGTCGCAACA GAAGGCTTGA ACAAGGACTG 
GAGTTTCAGA AACTGAATAT GGAAGCAGAT CGTATTGAGG CCACCATTTC TGGTCATGAA 
GCCAGACTTA AAGTCAGAGA CTTAGGGGAC TCTGTGGACA GTGTTCACAG TTTATTGAGC 
CGACAAGAAG AGCTTGAGGC TTTGCTTGAG GCTCTAGAGC GGTCTATTAT CCTCTTTCAA 
GACAAGTGCC AGGAGCTCAT CAGCAAGCGT AACTTTGCTG CTAAAGAAAT TCAACAGCGA 
TCAAAGGTCA TTCAAGATCA TTTCAAGAGG CTGAAAGAAA GTTGTAAATT AAGGAGGCTT 
GAATTACTTG CTTCGAAAAA GTACCAGGAA TTTTTAAGAG ATGCTGAGGA GATGCTGTTG 
TGGATGGATG AAAAGTTTGA GATCGCTGAG GATGAATCGT ACAGAGATCC AACCAACATC 
CTTCGTAAGC TTAAAAAACA TGAGGCAGCA GAAAAAGAGA TGCAGGCCAA TGCAGTTCGC 
ATAGACAGAC TAATTGAGAC TGGAGAGAAG ATGTTGGCAG ATGATCACTA CAACACCCAG 
AGCATTCAGA AAAAGACTCA AGAGGTACAC AAAAGGTGGA TTGAACTTCA GAGGAAGATG 
GCTGAAAGAG GGGACAAGCT GCGACAGGCT GGCCAACAGG AGCAACTCAT GGAGCTGCTG 
CAGGATGCTA AATTGAAGAT TGAGGCAATT GAGAGAATGC TACAAAATGC AATCAGGGGT 
CATGACCTCC GCTCCAGCAG ACAGCTACTA AAAGAGCACA AGCAGCTGGA AGAGGAAGCT 
CAAGAACTTG CTGAGAAAAT CAATTCTATT GTCACCCGTG CCAAAAACCT GGCCACCAAC 
CATTTTGACT CTCAGCGCAT TCTCAAGGAG ACTGACACTT ACCTGAAACT ATTTAAGTCT 
CTAGAGAAAC CTTTGGATCA TCGCAGGAGC CAGTTAGAGG CAGATGTGGA TCTATACAGC 
TTTTACCATG ATGTAGACCT AGAGCTAAAC TGGATATCAG AGCATCAGCC TGTAGCTGAT 
ACTACAAACT ATGAGAGATC ACTGGTTGGG GCAGTTAGCC TTCTTCAAAA ACATAAGGAC 
CTGCAGGCGG AGGTGAATGC CCACAGCAAG TACCTGCAGC GAGTACTGGA CAGAGGACGA 
GCCATGGCCC GATCCAATCA GTGGAATGGT CAGGAGGTGC AGCAGAGGTG TGAGCAATTG 
ATCGCAAAGT GGGAAGAGTT AGAGGAATCC TGCAAGAAAC GCTCCGGAGA ACTTAACAGG 
GCTGTTACAC GGGAACAGAT TCTGCTTGAC TGCACTGAGC TGGAAACCCT TGTGTCAGAA 
ACATCAGCTT TAGTCAGCAC TGATTATGGC AAGACTGAAT TAGCTACCCG CAGTCTAATA 
AAGCAACACG AGGGAGTGGA GGGGCAAATC GAGGTACTTG CTGCTCAAGT AGATGAACTC 
AAGTCCAATG TCAAGCAGGC TGTACGTGTG TGGGGTTTAG AGGAGTTGAA CAAGCCCTGC 
AACCACATTA AATCTAAGCT GAGTGAGCTT CAACACTCAG CGACAGTCAG AGAACAGAGG 
CTTCGAGAGA CTCTACACCT ACATGAATTT AAGCGTGAGA CTAGTGAACT GGAGGAATGG 
ATTGCTCAGC AGACACAGAT TGCAGCCTCT AATGATCTTG GGAGTGATTA TGAAAATGCA 
ATGCAACTGC GTGGGAGATT TGAGGTCTTC TTGAAGCAGC TGGAGGTGGG AAAGGAGAGG 
ATGCACACCT GTGAACAGTT GGCTGACAAA CTCATCAAAA GCAATCACCC TGAGAGTCGC 
TTCATCCGAG AAACACAGAA TCTGCTCAGA GAATCGTGGG ACGAGCTGCA GGATTTATCG 
AAAGATCGTA AAGAAAAGCT TCGTAAGTCT GAAAAATGTC ACAAATTCTA CAAAGACCTG 
ACCGATGCAC TGGGACAGAT CAAGGAGAGG CACAAAACCA TTCCTGAAGA CATAGCCAAA 
GATCTGAAAG GAGTGCAGTC ACAGCTACGC AAACATGAAG CTCTTGTGCA CGAGCTGGCT 
GGAACTGAGC AACAGCTGCA GGAGCTGTTG GATGGAACCG ATGCTATTTT GGGCATGTGC 
TCTCCTGAGC TGCGGGTGGG GTTGCAGGAA CTACAACAGG AGGTGGTGGA GAACTGGGAG 
CGGCTCCGCA TGCGGATGGA GCAGAGAGAA GAGGAGCTGC AATCGGCTAA AGACCGCTAC 
ATGTTCCTCA ACACGGCACA GGACTACTCC CTGTGGTGCT CTCAGGTGCT GAGCTGGATG 
AAGGCAGAGG AGTCCATTCG AGACGTTGCC ACCTGTGACC TGCAGTTGTT CCAGCACCAG 
CAGTTGTGGG CTGAGATTGT GGCCCGTGAG GAGACGTTTG CCCAGGCTGT GTCAATGGGT 
CAAGGCCTTC TGGAGAATGA CATACCTAAT GCTAGAGAGA TCCAGGAGAA ACTGAAAGCT 
CTGCAAGAAG AGAGAGAGAA GCTTTCCGAT AACTGGCAGC TCAGGCAGAA ATGGCTGGAG 
AACACATATC TGGAGCAGGT GTTTTACAGA GACACTGAGT ACATGGAGAA GATCACCAAC 
TCTCAAGAGA TCCAGTTGAA GAACAGTGAT TTAGGAACGA CAGTGGATGA TGTTGAAAGT 
TTGATTAAGC GCCATGAGGC CTTTGAAAAA CTTCTTCATT CTCAGGAAGA TAAGATGGTG 
GCTCTTCAGG AATCAGCAGA GCGTTTAAAG ACAGAAGGTT TAACACGAGA AAAAAACAGC 
AATATTAAGA ACAAACTCAA AGCTCTTCAG GAAAGGAGGG AACGGATTAA AGATCTGTCT 
GGCAAACGAA GAGAGGATCT TGAGGTCTCA AAGCTTCACT GTATTTTCAG CCGAGATGTG 
TCTGAGGCAG AGGAATGGAT CACAGATCGT ATGAACAAGA TACAAGATGA CTCCAAAATG 
GACATGAGCA ACCTTCAGAC TAAAATGAAG ATGCTGCAGA AGCATCAGGT GTTTGAAGCT 
GAGATTCTGG CCCATGGAAA TATCATTGAA TCTGTCCAGC AGGCTGGAAA TGAGCTGGTG 
ACCATGCATC ACCCAAAATC AAAGGAGATA CGCCAGACTG TTTCAGCGCT CATCTCTCAT 
TGGAAGGCTC TAAAGCAAGA GCTTGCGGTG CGTGGAAAGG TTCTAGAGGA TAACCGTGAT 
TTTCTTGAGT TCCTGCAGAA AGTGGAACAG GTTGAAGTTT GGATTAGGCA AAAGGAGGTG 
ATGATAAATG TTGGAGATGT CGGGGAGGAT TATGAACACG GTCAGCAGCT GCTGAAGAAG 
CTCAGTGAAT TCAGAGGGTC AGGCTCTGGA GATGTGACTG TTGACGATGC TCACATCAAG 
ACAATCAACA CTTTGGCCGC TCGACTTGAG AGACAGAACA GTGATGAGCT GGTGACTGTG 
AAAAAGCGTA GGCAGCAGCT CAATGAAAGA TGGAGGAATT TCCATGGCAA CCTGAGCAGC 
TATAAGAGGA AGCTGGAGGC AGCGTTGGAG GTCCACGCAT TGATCCGAGA ACTAGAGGAG 
GTCCGGGACA GAGCTGGAGA GAAGATGCTG TTGCTCCAGG GTCAGGGTTG CGGGTTGGAT 
GTTGAAAGTG TGGAGAACCT CATTCGCAGA CATGAAGAGA TGGAGAGGGA GGCCAGGGTC 
ATCCAGGAGA GAGGCGCTGC CCTGGAAAAG GAGACTAAAG ATCGGTTGAA GAGACACTGC 
GATCTGTCTG ACAAACTTAG CAAGAAGCAG GAAGAGGTCA AAATTGCTTT AGTCAAGCTG 
GACAAGGAAA TCAAACGAAG GAAGGAGCGA TTGCAGGAGT CTCATCAGCT CCAGCTGTTT 
AAAGCGAATC AGCGTCTGCT TTTGGACTGG ACCCTCAAGC AAAGTGATGA GATGGTGAAG 
AAAGGCCTGC CTAAGAATAA AACCGAAGCT GAGAGTTTCA TATTAGAACA TCACGACTGG 
AAGGCTGAAA TTGATGCTCG TGGCGATCGG ATAGATTCAG TTAAGAGTTT CGGACAGAAT 
CTTGTGAAGT CTGGACACAG TGATGCAGCA GAGATTAAAG AAGCCCTACG CAAGCTGGAT 
GATGCCAAGA CAATGCTTAC CCGAGCTTGG GAGGACCGAA GAAAAACTCT GGATCAAGCC 
CTGAAGCTCC AGATATTCCT GGGCTATGCT GACCAGACTG AGAGCTGGAT GAGTAACAGG 
GAGGCTTTCT TGATCAATGA AGATCTAGGG GGTTCATTGT CGGAGGTGGA GGCTCTACAG 
AGGAAGCTGG CTCTGTTTGA GAACTCTCTA GAAGCTCAGA TGGAGCAGGT TTCAGAGGTT 
GACAGATACG CTCAGCAGCT CATACAGGCA AACCACTACG ACTCTGACAA CATCAAGAAG 
AGGACAAAAG CTATTCTGCT CAGGAAGGAA AGAATACTGG AAATCAGCAA ATCCCGGCGA 
AAAGCTCTAG AAGAATCTCT TCAGTTGCAA AAGTTCCTGG AAGCAAGTTA TGAGATTTCC 
TCATGGCTGA ATGAGAAGAA CTCAGTGGCT GTTGACGAGA GTTGGAAAGA TCCTATAAAT 
CTGCAGGCTA AGCTACTGAA ACACCAGAGC TTTGAAGCAG AGATTCTGGC CAACCGCAAC 
AGAGTGCAGG CACACATTAA AGATGGTGAT AACATGCTGG CTTTCAGTCA TCCTGCTAAA 
AGCAAAATAA AACCACGAAT AAAGGATGTC GATGACAACT GGGACCAGCT TTTAAGCAAC 
TGCAAGCATA AGAAGACACG TCTACAGCAA GCGTATCAGG CCTTACAGTT CCAGCGTTCA 
TTAGGTGACA TTGAGGAGTG GCTTGGGTCG GTGGAGGTGG AAGTGGCGAA TGACGACTAT 
GGAGGTGATC TGGCCTCTGT TAGCAGGCTG CTTAAGGCTC TACAGGGTCT GGAGGAAATG 
GTAGACGCGC ACATGGAGAA AGTCCAGGGG CTAGTGGACA CTGCCAAAGA CTTCAGCTCT 
CAGGGCAACT TCCTCGCAGA TAATATACAG CAGAGGGTTT GGGAGGTGGT CAACAGGTAT 
AATGGCCTGG CTGAGCCGAT GCAGACCCGC AGGGAGACTC TGGAGTCGTG GCAGCTGCTG 
TTCCAGTTCT ACAGGGACTT AGAGGATGAG CTGCTGTGGA TTGAGGACAA GCTGCAGGCC 
ACGGCCACCA AAGACTGGGG CACCTCCCTA CAGAGCATAC AATCTATAGT CAAAAAACAT 
CTGGTTGTGA TGCAGGAAAT TGAGAACCGA ACTCCTCTAA TAATGGCTGT ACAGGAGGCA 
GGACAGAATT TGGTGCGAGG ACGACACTTT GCATCACGTG AGATCAGTGA AAAACTTTCA 
GGGTTAAAGA AGAACTTTGA CGCCCTGAAG AAGGAGTCAG CGAATAAAGA TCGCCTGCTT 
CAACAAGCAC TGAAAATCCA GAGCTTTCTC TCAGAGGTTT CCCAGTTGGA GCAGTGGATG 
GAGGAACAGA AGCCTGTTTT AGAGTCCCGC GACTATGGAA AGAATGAGGA GGCCACTGAA 
GCTTTTCTCA GGAAGCTGGA CACAGTTGAC CTGGAGTTGG AAAGCCAGCG TGGAAAAGTA 
GAGGCTCTCC TGAAAACCGG CACAGATTTA GAGAAGTCTC AGCATCCCAA CAGCCATTTA 
GTGAGTAAGA GCCTTGAAGA TATGCAGGGT GAGTTTGAGA CACTACTGCA ACTGTCTGGT 
GAACGTCGCA CAGAGCTGGA GAATCAGTAC AATTTCTACA TATTTGAGAG AGATGCCAGA 
GACCTACAGA CCTGGCTCCT TTCTCGGAAG ACCATAGCAG AATCTGATGA TTATGGAAAA 
GATCTGGAAG GAGTGGAAGC ACTGCAAAAG AAGTTTGAGG ATTTTTCAGA AGAGGTTGGG 
ACTCTGGGCC AGAGTAAGCT GAGCACTGTT CAAAAGCTAA AGCAAAGTGT GCAGTCAGCA 
GATGCACAAC AAAAAGAGAA AGATCTACTC AAGCTCTGGG AGGATTTAAA CAAGGCTATG 
AAAGCAAGAG CAGAGAACCT GCATTCAGCT CGAGAAGTTC ACCAGTTTGA ACATGACCTG 
GATGAGCTTC GGATCTGGAT GAATGAGAAG GAAGTTGCTC TGGACTCTGA GGAGCTGAAG 
AATGACCTGC TTAGTGTCCA AGCTCTCATC CGTCAACATG AAGGACTGGA GAGAGACCTT 
GCAGTGATAG AAGAAGATGT GTCTCGTCGA AAAGAGGAGG GAAAAGCTTT GGTCCGCAGG 
TCACCTCGAG TAAGGGACAG TTTGAGCAAG AGACTCCAGG AAGTGGAGGA GATCTGGAGA 
AACCTGCTGG AGAAAGCCAA CCAGCGCAGA CAAAGACTCC AACAGGCAGA GGCCGTTCAA 
AGATATCTGA TTGAATGGAG AGAGATGATG GGCTGGCTGA AAGAGACACT GTCTCTGATG 
ACAGGGGAGG GATTCGGAGG CAAGGTTAAA GACCTGGAAC AGATGATTAA AAAACATGAT 
GAGTACCGCA CACAGATTGA CAGACAACTG GACAAATCGG AAATGGTTAA AAATGATGGG 
AGACGTCTGA TGGAGGAGGG GAACTCATGG AGTCATGAGC TTGAGGAGCG TCTAGCCGAG 
CTCAAGGATC TGGAGGTGCG GGTGCTGGAG GCCTGGACTG AAAGGAGAGC TCAGTATCAG 
GAAGACATAG AGCTCCAGCA ACTGCAGAGG GAACTGGAGC AGGCCGAGCA CTGGCTCAAC 
ACTTATGAGA GCGCGCTCAG AGCAGAGGAC TATGGGGAAT CAGTTTCAGA TGTTCTGGAT 
TTGATGAAAA AGCAGGAAGA TCTGGAGGCC ATGATTCAAG CACAGAGTGA GAGATTCAAT 
GCCCTTCAAG CCAGAAGAAC ACAGAGGGAA AATAAGATGA AAGAAATTCA AAGAGGTGAC 
AGTACAGACA GCAGCAGGGA CAAGCCTGTT CGTGTAACAT CCCTAAAGCG AAAGCCATCT 
GAGCGGCCAG CATTGCCCCC TCGTCCCTTG TTGACCAGTC CAGTCCTAAA GAACAGCGAT 
TCACCCACAC CTTCCCGAGT CCCTACCCCC AGGTCTGCCT TGTGGGAAAG CAGAAGTGAT 
AAAACTGATT CTCCTGTGGT GTCACGAGTG GCAGCAGGAC TCAGAAGAAA GAGCAGCAGT 
GACAGTGATA GTGTAACATC ACCTGACAAA CCATCCACTT CTGGTCAGGA TAGCCCTGAC 
TCCTCCTCCA CTGCGGTTTA CAGACCTCAT TATCCACACA ATGCTTCTAA GGTTAAACAT 
ACCACATCTT CACAAGATGA AGTCGATACT CTAAGAGAGA CTGAATCCTT TTTGAAAACC 
ATTGATAAAC CAAAAATCAC TCCTAGGCGT CAGAAACCAT CAACTCCTGA TAAAGAGTTG 
TGGGGTCCTC CTACATCCAA ACCACCTGAG CCACCCATTA AACCTTCACA ATCATTGTCC 
AGATCTCCTC AGTCACTGTC CAGATCTCCT GAACCACTGT CCAGATCTCC AGAGCCATTG 
CCTAGATCTC CTGAGCCACT GTCCAGATCT TCCCAGTCAC AGCCCAGATC TCCTGAACCA 
CTATCCAAAT CGCCTGAACC ACTATCCAGA TCTCCTGAGG CACTGTCCAG ATCTCCCGAG 
CCACTGCCCA GATCTCCTCA GTCACTGTCT AGATCTCCTG AGCCACTGTC CAGATCTCCT 
GAGTCACCGT ATATTCCTCC ACTCACAAAA CCTCCATCCA CAAACCCTCC TGATCCACCA 
TCCAAGGCCA CAGAATTGGT GGACAACCAA GACTCAAAAG ATAGGAACCT TCCTCAATCC 
ACTCCACCAT CAGTCCCTCA GTCATCACCT CAATCTTTCG TTTCCAACCC AGAACCACAA 
GCGTCCTCTT CTGTGCAATC CTCAATAGTC TTTCCAGCTG CTTCCACCAT GATCAGAGCA 
GAAGATGCCA AAATGGAGGG TACGTTGGAG ATTAAACTGA AGCAAGGAGG AAATACAGGC 
TTGGATCACT GGGAAACGGT CTATGCACTA CTGGAAGAGG AGACACTCAA TCTGTTTAAA 
GACCAAGATG CCGCCACTGA GAACTGTACA CGCTGGCCAC CGATCAATTT GGCTGGAGCT 
ATTTGTAAAG ACAACCCTTT TTACAGGAGA AAGGAGCACA CGTTCAAACT CATGCTGAGT 
GATGGCAGTC ACTATCTGTT TGCTGCATCA TCTCAAGGAG AACAGCAGAA GTGGCTGAAG 
GAGCTCCAGG AATGTATTAA TCCAACTGTG TCTTCAGACA ATCCTGGAAT GAAGAGAGAA 
TCAAGCAGTG AAAGCGTAGA GAAGAGCGAG TCTGTGCCAG TCGACACATC TGACACACAA 
GACCAGGAGA AGGAAGACTC CACCGAGAGC TGTGAGACAG AGAGAGAACC ACCACCAAAA 
CCTCCACACA CGTACTACAA CAAACACAAA TACCCTGACG GAGGAGAGAG CCAAGACTCA 
GTGAGTGTGT CTCAAAATAT AAAAGGGCTA CAGCCTCCCA CATTCGCTCC TCCTCCTCCT 
CCAACACAAA ACCAGACAGA GAATGAAGCC AAGGCCAAAA ACAGGAGCGG ATTTATGAAG 
TTTTTCAGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn5 ( XM_017351710.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207199.1 Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), mRNA.

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CloneID	ODa55643
Clone ID Related Accession (Same CDS sequence)	XM_017351710.1
Accession Version	XM_017351710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	spectrin beta chain, non-erythrocytic 5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACGGTGC AGAAGAAGAC CTACACAAAA TGGATGAACA GCGTCTTCTC AAAGAGTGGG 
GAAAAGGTTG TCTTGACTGA TGTATTCACC GAATTAAGAA CTGGTGTGCA TCTCGTGCGA 
CTTCTGGAGC TCATTTCTGG AGAGAAGTTG CCCACTCCCA GTCGACGCAC ACTAAGAGTT 
CACTGTCTGG AAAACAACAG CATTGCCATC ACTTTCCTCA AATCAAAGAT CCGTGTAGAT 
CTGATTGGTC CAGAAAATGT GGTGGATGGA GACAGAACCC TGATCCTGGG TCTAATCTGG 
ATCATCATTC TCCGCTTCCA GATTGGGAAC ATCAATATAG TTGAGGCCGG CGGGGATGCC 
AGTGCGGCTC GGCGCTCAGC CAAAGAAGCT CTGCTCATCT GGTGTCAACG TAAGACTGCC 
GGTTACACCA ATGTAAATGT GCAAGACTTC TCAGGCAGCT GGAGAGATGG ACTGGCCTTC 
AATGCTCTAA TTCATGCCCA TCGGCCTGAT CTTTTTGACT ACAACCGACT CCGCCAGGAT 
GATCCAAAAA GGAGCCTCAC CCATGCTTTC GCTCTGGCAG AGGATGAATT TGGCATAATG 
CAGCTCCTCG ATGTTGAAGA TGTGATGGTG CCCCATCCTG ATGAGAAGTC CATCATGACT 
TACGTCTCTC TGTACTATCA CTACTTCTCA AAAATGAAGC AGGGGCAGAC TATCCAGAAG 
AGGATAGCTA AGATTGTGGA CCTTCTGAAA GAGCTGGATG ATATGAAGGG TCTGTATGAG 
AGTATGGTGT CTGACTTGCT ACGCTGGATC AAGACCAAGG TGGTGGAGCT CAATGACCGT 
TCCTTCCCCA ACTCCCTGCG AGAAATGCAG CATCTGGTAG CTGCCTTTAA GAAATACCGT 
ACTATTGAGA AGCCTCCCAA ATACCAGGAA CGGGGGGCCA TTGAGGCACA CCTCTTCGAC 
TTACGAACCA AGCTGAGGGC AAACAACCAG AGGGACTACA TTCCTCCGGA AGGCAAGACC 
TTGGGGGATA TTGAAAGGAA CTGGACCTTG CTAGAGCGAG CAGAACACCA GCGAGAACGG 
GTTCTCCAGA GTGCACTGAT GAGGCTGGAG CAGCTGGAGC AGTTGGCCCA GAAGTTTGGC 
CGTAAAGCGA CGCTGAGGGA GAGCTACCTG GAGGACACTC TGCAACTGAT TCAACAACAA 
GACCTGGGAG GACTGCAGAG GCTGGAGGAG GCTGAGATAG CAGCTCGCAG ACTGGAGGCC 
CTGGGAGCTG ATGTTTTGGC ACATGAACCA CGTTTCCGTG CTTTAAGTGA AATGGCTGCT 
GTCATCGAGA GGGATAACTA CCACAGCAAA GTACAAGTGA TTCGCAGAAA TGTGGACATC 
ATGAACCGCT GGAAGGATTT GAAGCAGGTT CTGCAGGAGC AGAGAGCACA TGTTGGAGAT 
GCGGCAAATA CATTTGCTGT CCTTAGAGAC ATTGAGTTAA TATCAATGGA CCTCAAGGAA 
TTACAGGCTC AGGCAGATTC CTCGGACATG GGAAAACAGC TGCCAGATGT GGTAGCCCTA 
CTGCAAAAGC AGGACTTGAT TGACACTCAG ATCATCTCAC TTGGTGAAAC TCTGAATGCC 
ATCAGTAGTA GCAGTGCCTT AAAAGTTAAA CACAAAGGTG CCACAAAAGT CCAGAGAGAA 
CTTAAAGATC TAAATAGCCA TTACAACTCC CTACTGGGTC TCAGCAAGAA CAGGAGAAAA 
GCTCTTGAGG GACAGCTGAA GCTCTTTGAG TTTTTCCATG ATTGTGAAGA AGTAGAAGCA 
TGGATCTATG AGAAATGGGT CCTCTTACAG GCAGCATCAC TAGGGAGAGA CCTCAGTCAA 
ATTCAGCAGG CCATTCAGAA CCACAAGGCT TTGGAGGCAG AGATTCAGTC ACAGGAATCT 
CTGTGCTCTG GGGTTGTATT TAGAGGACAA GAGCTTTGTA GAGGGAGACA CCCTAATGAG 
AATGACATCC AAAAGTGGAT TCGGACTCTT CAGAAACAGT GGCAGCAACT TAAAGAACAA 
GTCACCATCC GAAAGAACCG GCTGCATGCT GCCAATGTCA TCAAGCAGTA TTTTGCAGAC 
ATTACTGAAG CCAGCTCTTG GCTTGATGAC CACAAACCAC TTCTCATTGA CGAGGATTAT 
GGAAAGGATG AGGCCAGCTC TACTGCTCTG CTCCAGCGTC ACCAACGAGT GGAGAAAGAA 
ATGGAAGCAT ATTCCTCAGA AGTCAAACGA CTGGGTGAGC AGGCCAAGAG CGCTGCCCAA 
CTTGCTCCTC TTACAACTGA AACCCAGCAA ACGAGAATGG TCCACTACAG TGATTCATCA 
GATGGCGAAG ATGAGAAGGA GAAAAAAGCT GACAAAGGAG GCAAAGCAGT CAGCAAGATT 
TTACAACAAG AACAAGCAAT GATTAGGTTT AAATACAGAG GCCCTAAACT AATCTGGGAC 
AGAGGAGAAA TATTGACAAT TATCAGCAGG GAAAAGGATG ATAAGTTGCT TCTTCGGGAC 
AGCAGAGGAA ATGAGCAACT AGTCTCTTCC ACATACATCA GGGAGCTGCC AGTTTCTCAG 
GAAGCACCAC CTGAAACTAC AAACGGAGTA GCTGAGAGTC CAAAAAGAAA AGTGAGCCGC 
CCTCGCCGCA CTCGATCCAT GCGGCGTGGC ACAGCTGAAA TATCTACCTC AACCTTACCA 
GACCCACATT TCCAAAAAGA CACAATAGAG AGCACACAAA ACAGTCTGGA AAAGGATTTC 
AACCGCCTTT ACAATTTAGC ACAGTCAAAA AATAAGATCC TGGAGGACAC TGCACGACTA 
CACAGATTCT ACAATGCTTG TGAGGAGTTT GAGTCCTGGA TGGGAGACAA GGAGAATGTC 
CTCAACACTT TCAGCTCCAA TTCCAACAAC TTAGAGGTTG TACAAGCCAA ATATGAGAAT 
TTTGTAACTG AACTAGCCAG TGGTAGAAAG CAGCTTGATG ATATTACCAA GTTTGGAGAT 
GAGCTATTGA AACAACAGCA CAGCAGGAAA AAAGACATTC AGCAAAGACT TGGAATTGTT 
ACTCGCAGAT GGGAACGTAT TCAGAAACTA AAGGATGAGA TGGCTCATGA GCTTCTGAGT 
TCTGCTGATG TGAAGTCCTT CCTGCAGACC TGCCAGGAAG TCCAAGCTCA GCTCCAGCAC 
AGACTCGCCC AGCTTGACAC ACCTGATGTG GGGAGCTCAC CATCAGCCTT ACAAGCTGAG 
GAACACAGAC AAGCCCAAGC TGAAAGGGAC ATAGAAGCCC TTGAAAGGAA AATAGACTAT 
CTTAAAAGTG TGGCCAAGAT GAAAAAGGAC TGTAGTCCTG CAGAAAGTGC TGCTATCATG 
GAAGAAGTAC ATGCTCTGGA AGACTTGTTG CGGCAGTTAA AGGCTCAAGC CATTCAAAGA 
CAACGAATGC TTGAAGAAGC CAGACGCTTG CAACTCTTTC AAAAAGAGAC CAGAGACCTG 
TTGATGTGGG CTGAGGCAAC ACAGGAGCAT CTTCTGGAAG AGGAGACAGG ATCTGATGTA 
GCATCGGCTC AAGCCTTGTT GAAGGAGAAC CTTGATCTCA AGCAGGAGAT TGAACTGCAG 
AAAGCAAAAT TAAAAGATAT GGAGAGGCTG GGTCAGTCTC TTGACGTGGC ATCTGGAGAG 
AAAGGAGGTA AAGATGTTCA GCAGACACTT ACAAAGTTGG ATCATGAGTG GAGCCAGCTG 
GACAAACTGT GGTCTAGTCG CAACAGAAGG CTTGAACAAG GACTGGAGTT TCAGAAACTG 
AATATGGAAG CAGATCGTAT TGAGGCCACC ATTTCTGGTC ATGAAGCCAG ACTTAAAGTC 
AGAGACTTAG GGGACTCTGT GGACAGTGTT CACAGTTTAT TGAGCCGACA AGAAGAGCTT 
GAGGCTTTGC TTGAGGCTCT AGAGCGGTCT ATTATCCTCT TTCAAGACAA GTGCCAGGAG 
CTCATCAGCA AGCGTAACTT TGCTGCTAAA GAAATTCAAC AGCGATCAAA GGTCATTCAA 
GATCATTTCA AGAGGCTGAA AGAAAGTTGT AAATTAAGGA GGCTTGAATT ACTTGCTTCG 
AAAAAGTACC AGGAATTTTT AAGAGATGCT GAGGAGATGC TGTTGTGGAT GGATGAAAAG 
TTTGAGATCG CTGAGGATGA ATCGTACAGA GATCCAACCA ACATCCTTCG TAAGCTTAAA 
AAACATGAGG CAGCAGAAAA AGAGATGCAG GCCAATGCAG TTCGCATAGA CAGACTAATT 
GAGACTGGAG AGAAGATGTT GGCAGATGAT CACTACAACA CCCAGAGCAT TCAGAAAAAG 
ACTCAAGAGG TACACAAAAG GTGGATTGAA CTTCAGAGGA AGATGGCTGA AAGAGGGGAC 
AAGCTGCGAC AGGCTGGCCA ACAGGAGCAA CTCATGGAGC TGCTGCAGGA TGCTAAATTG 
AAGATTGAGG CAATTGAGAG AATGCTACAA AATGCAATCA GGGGTCATGA CCTCCGCTCC 
AGCAGACAGC TACTAAAAGA GCACAAGCAG CTGGAAGAGG AAGCTCAAGA ACTTGCTGAG 
AAAATCAATT CTATTGTCAC CCGTGCCAAA AACCTGGCCA CCAACCATTT TGACTCTCAG 
CGCATTCTCA AGGAGACTGA CACTTACCTG AAACTATTTA AGTCTCTAGA GAAACCTTTG 
GATCATCGCA GGAGCCAGTT AGAGGCAGAT GTGGATCTAT ACAGCTTTTA CCATGATGTA 
GACCTAGAGC TAAACTGGAT ATCAGAGCAT CAGCCTGTAG CTGATACTAC AAACTATGAG 
AGATCACTGG TTGGGGCAGT TAGCCTTCTT CAAAAACATA AGGACCTGCA GGCGGAGGTG 
AATGCCCACA GCAAGTACCT GCAGCGAGTA CTGGACAGAG GACGAGCCAT GGCCCGATCC 
AATCAGTGGA ATGGTCAGGA GGTGCAGCAG AGGTGTGAGC AATTGATCGC AAAGTGGGAA 
GAGTTAGAGG AATCCTGCAA GAAACGCTCC GGAGAACTTA ACAGGGCTGT TACACGGGAA 
CAGATTCTGC TTGACTGCAC TGAGCTGGAA ACCCTTGTGT CAGAAACATC AGCTTTAGTC 
AGCACTGATT ATGGCAAGAC TGAATTAGCT ACCCGCAGTC TAATAAAGCA ACACGAGGGA 
GTGGAGGGGC AAATCGAGGT ACTTGCTGCT CAAGTAGATG AACTCAAGTC CAATGTCAAG 
CAGGCTGTAC GTGTGTGGGG TTTAGAGGAG TTGAACAAGC CCTGCAACCA CATTAAATCT 
AAGCTGAGTG AGCTTCAACA CTCAGCGACA GTCAGAGAAC AGAGGCTTCG AGAGACTCTA 
CACCTACATG AATTTAAGCG TGAGACTAGT GAACTGGAGG AATGGATTGC TCAGCAGACA 
CAGATTGCAG CCTCTAATGA TCTTGGGAGT GATTATGAAA ATGCAATGCA ACTGCGTGGG 
AGATTTGAGG TCTTCTTGAA GCAGCTGGAG GTGGGAAAGG AGAGGATGCA CACCTGTGAA 
CAGTTGGCTG ACAAACTCAT CAAAAGCAAT CACCCTGAGA GTCGCTTCAT CCGAGAAACA 
CAGAATCTGC TCAGAGAATC GTGGGACGAG CTGCAGGATT TATCGAAAGA TCGTAAAGAA 
AAGCTTCGTA AGTCTGAAAA ATGTCACAAA TTCTACAAAG ACCTGACCGA TGCACTGGGA 
CAGATCAAGG AGAGGCACAA AACCATTCCT GAAGACATAG CCAAAGATCT GAAAGGAGTG 
CAGTCACAGC TACGCAAACA TGAAGCTCTT GTGCACGAGC TGGCTGGAAC TGAGCAACAG 
CTGCAGGAGC TGTTGGATGG AACCGATGCT ATTTTGGGCA TGTGCTCTCC TGAGCTGCGG 
GTGGGGTTGC AGGAACTACA ACAGGAGGTG GTGGAGAACT GGGAGCGGCT CCGCATGCGG 
ATGGAGCAGA GAGAAGAGGA GCTGCAATCG GCTAAAGACC GCTACATGTT CCTCAACACG 
GCACAGGACT ACTCCCTGTG GTGCTCTCAG GTGCTGAGCT GGATGAAGGC AGAGGAGTCC 
ATTCGAGACG TTGCCACCTG TGACCTGCAG TTGTTCCAGC ACCAGCAGTT GTGGGCTGAG 
ATTGTGGCCC GTGAGGAGAC GTTTGCCCAG GCTGTGTCAA TGGGTCAAGG CCTTCTGGAG 
AATGACATAC CTAATGCTAG AGAGATCCAG GAGAAACTGA AAGCTCTGCA AGAAGAGAGA 
GAGAAGCTTT CCGATAACTG GCAGCTCAGG CAGAAATGGC TGGAGAACAC ATATCTGGAG 
CAGGTGTTTT ACAGAGACAC TGAGTACATG GAGAAGATCA CCAACTCTCA AGAGATCCAG 
TTGAAGAACA GTGATTTAGG AACGACAGTG GATGATGTTG AAAGTTTGAT TAAGCGCCAT 
GAGGCCTTTG AAAAACTTCT TCATTCTCAG GAAGATAAGA TGGTGGCTCT TCAGGAATCA 
GCAGAGCGTT TAAAGACAGA AGGTTTAACA CGAGAAAAAA ACAGCAATAT TAAGAACAAA 
CTCAAAGCTC TTCAGGAAAG GAGGGAACGG ATTAAAGATC TGTCTGGCAA ACGAAGAGAG 
GATCTTGAGG TCTCAAAGCT TCACTGTATT TTCAGCCGAG ATGTGTCTGA GGCAGAGGAA 
TGGATCACAG ATCGTATGAA CAAGATACAA GATGACTCCA AAATGGACAT GAGCAACCTT 
CAGACTAAAA TGAAGATGCT GCAGAAGCAT CAGGTGTTTG AAGCTGAGAT TCTGGCCCAT 
GGAAATATCA TTGAATCTGT CCAGCAGGCT GGAAATGAGC TGGTGACCAT GCATCACCCA 
AAATCAAAGG AGATACGCCA GACTGTTTCA GCGCTCATCT CTCATTGGAA GGCTCTAAAG 
CAAGAGCTTG CGGTGCGTGG AAAGGTTCTA GAGGATAACC GTGATTTTCT TGAGTTCCTG 
CAGAAAGTGG AACAGGTTGA AGTTTGGATT AGGCAAAAGG AGGTGATGAT AAATGTTGGA 
GATGTCGGGG AGGATTATGA ACACGGTCAG CAGCTGCTGA AGAAGCTCAG TGAATTCAGA 
GGGTCAGGCT CTGGAGATGT GACTGTTGAC GATGCTCACA TCAAGACAAT CAACACTTTG 
GCCGCTCGAC TTGAGAGACA GAACAGTGAT GAGCTGGTGA CTGTGAAAAA GCGTAGGCAG 
CAGCTCAATG AAAGATGGAG GAATTTCCAT GGCAACCTGA GCAGCTATAA GAGGAAGCTG 
GAGGCAGCGT TGGAGGTCCA CGCATTGATC CGAGAACTAG AGGAGGTCCG GGACAGAGCT 
GGAGAGAAGA TGCTGTTGCT CCAGGGTCAG GGTTGCGGGT TGGATGTTGA AAGTGTGGAG 
AACCTCATTC GCAGACATGA AGAGATGGAG AGGGAGGCCA GGGTCATCCA GGAGAGAGGC 
GCTGCCCTGG AAAAGGAGAC TAAAGATCGG TTGAAGAGAC ACTGCGATCT GTCTGACAAA 
CTTAGCAAGA AGCAGGAAGA GGTCAAAATT GCTTTAGTCA AGCTGGACAA GGAAATCAAA 
CGAAGGAAGG AGCGATTGCA GGAGTCTCAT CAGCTCCAGC TGTTTAAAGC GAATCAGCGT 
CTGCTTTTGG ACTGGACCCT CAAGCAAAGT GATGAGATGG TGAAGAAAGG CCTGCCTAAG 
AATAAAACCG AAGCTGAGAG TTTCATATTA GAACATCACG ACTGGAAGGC TGAAATTGAT 
GCTCGTGGCG ATCGGATAGA TTCAGTTAAG AGTTTCGGAC AGAATCTTGT GAAGTCTGGA 
CACAGTGATG CAGCAGAGAT TAAAGAAGCC CTACGCAAGC TGGATGATGC CAAGACAATG 
CTTACCCGAG CTTGGGAGGA CCGAAGAAAA ACTCTGGATC AAGCCCTGAA GCTCCAGATA 
TTCCTGGGCT ATGCTGACCA GACTGAGAGC TGGATGAGTA ACAGGGAGGC TTTCTTGATC 
AATGAAGATC TAGGGGGTTC ATTGTCGGAG GTGGAGGCTC TACAGAGGAA GCTGGCTCTG 
TTTGAGAACT CTCTAGAAGC TCAGATGGAG CAGGTTTCAG AGGTTGACAG ATACGCTCAG 
CAGCTCATAC AGGCAAACCA CTACGACTCT GACAACATCA AGAAGAGGAC AAAAGCTATT 
CTGCTCAGGA AGGAAAGAAT ACTGGAAATC AGCAAATCCC GGCGAAAAGC TCTAGAAGAA 
TCTCTTCAGT TGCAAAAGTT CCTGGAAGCA AGTTATGAGA TTTCCTCATG GCTGAATGAG 
AAGAACTCAG TGGCTGTTGA CGAGAGTTGG AAAGATCCTA TAAATCTGCA GGCTAAGCTA 
CTGAAACACC AGAGCTTTGA AGCAGAGATT CTGGCCAACC GCAACAGAGT GCAGGCACAC 
ATTAAAGATG GTGATAACAT GCTGGCTTTC AGTCATCCTG CTAAAAGCAA AATAAAACCA 
CGAATAAAGG ATGTCGATGA CAACTGGGAC CAGCTTTTAA GCAACTGCAA GCATAAGAAG 
ACACGTCTAC AGCAAGCGTA TCAGGCCTTA CAGTTCCAGC GTTCATTAGG TGACATTGAG 
GAGTGGCTTG GGTCGGTGGA GGTGGAAGTG GCGAATGACG ACTATGGAGG TGATCTGGCC 
TCTGTTAGCA GGCTGCTTAA GGCTCTACAG GGTCTGGAGG AAATGGTAGA CGCGCACATG 
GAGAAAGTCC AGGGGCTAGT GGACACTGCC AAAGACTTCA GCTCTCAGGG CAACTTCCTC 
GCAGATAATA TACAGCAGAG GGTTTGGGAG GTGGTCAACA GGTATAATGG CCTGGCTGAG 
CCGATGCAGA CCCGCAGGGA GACTCTGGAG TCGTGGCAGC TGCTGTTCCA GTTCTACAGG 
GACTTAGAGG ATGAGCTGCT GTGGATTGAG GACAAGCTGC AGGCCACGGC CACCAAAGAC 
TGGGGCACCT CCCTACAGAG CATACAATCT ATAGTCAAAA AACATCTGGT TGTGATGCAG 
GAAATTGAGA ACCGAACTCC TCTAATAATG GCTGTACAGG AGGCAGGACA GAATTTGGTG 
CGAGGACGAC ACTTTGCATC ACGTGAGATC AGTGAAAAAC TTTCAGGGTT AAAGAAGAAC 
TTTGACGCCC TGAAGAAGGA GTCAGCGAAT AAAGATCGCC TGCTTCAACA AGCACTGAAA 
ATCCAGAGCT TTCTCTCAGA GGTTTCCCAG TTGGAGCAGT GGATGGAGGA ACAGAAGCCT 
GTTTTAGAGT CCCGCGACTA TGGAAAGAAT GAGGAGGCCA CTGAAGCTTT TCTCAGGAAG 
CTGGACACAG TTGACCTGGA GTTGGAAAGC CAGCGTGGAA AAGTAGAGGC TCTCCTGAAA 
ACCGGCACAG ATTTAGAGAA GTCTCAGCAT CCCAACAGCC ATTTAGTGAG TAAGAGCCTT 
GAAGATATGC AGGGTGAGTT TGAGACACTA CTGCAACTGT CTGGTGAACG TCGCACAGAG 
CTGGAGAATC AGTACAATTT CTACATATTT GAGAGAGATG CCAGAGACCT ACAGACCTGG 
CTCCTTTCTC GGAAGACCAT AGCAGAATCT GATGATTATG GAAAAGATCT GGAAGGAGTG 
GAAGCACTGC AAAAGAAGTT TGAGGATTTT TCAGAAGAGG TTGGGACTCT GGGCCAGAGT 
AAGCTGAGCA CTGTTCAAAA GCTAAAGCAA AGTGTGCAGT CAGCAGATGC ACAACAAAAA 
GAGAAAGATC TACTCAAGCT CTGGGAGGAT TTAAACAAGG CTATGAAAGC AAGAGCAGAG 
AACCTGCATT CAGCTCGAGA AGTTCACCAG TTTGAACATG ACCTGGATGA GCTTCGGATC 
TGGATGAATG AGAAGGAAGT TGCTCTGGAC TCTGAGGAGC TGAAGAATGA CCTGCTTAGT 
GTCCAAGCTC TCATCCGTCA ACATGAAGGA CTGGAGAGAG ACCTTGCAGT GATAGAAGAA 
GATGTGTCTC GTCGAAAAGA GGAGGGAAAA GCTTTGGTCC GCAGGTCACC TCGAGTAAGG 
GACAGTTTGA GCAAGAGACT CCAGGAAGTG GAGGAGATCT GGAGAAACCT GCTGGAGAAA 
GCCAACCAGC GCAGACAAAG ACTCCAACAG GCAGAGGCCG TTCAAAGATA TCTGATTGAA 
TGGAGAGAGA TGATGGGCTG GCTGAAAGAG ACACTGTCTC TGATGACAGG GGAGGGATTC 
GGAGGCAAGG TTAAAGACCT GGAACAGATG ATTAAAAAAC ATGATGAGTA CCGCACACAG 
ATTGACAGAC AACTGGACAA ATCGGAAATG GTTAAAAATG ATGGGAGACG TCTGATGGAG 
GAGGGGAACT CATGGAGTCA TGAGCTTGAG GAGCGTCTAG CCGAGCTCAA GGATCTGGAG 
GTGCGGGTGC TGGAGGCCTG GACTGAAAGG AGAGCTCAGT ATCAGGAAGA CATAGAGCTC 
CAGCAACTGC AGAGGGAACT GGAGCAGGCC GAGCACTGGC TCAACACTTA TGAGAGCGCG 
CTCAGAGCAG AGGACTATGG GGAATCAGTT TCAGATGTTC TGGATTTGAT GAAAAAGCAG 
GAAGATCTGG AGGCCATGAT TCAAGCACAG AGTGAGAGAT TCAATGCCCT TCAAGCCAGA 
AGAACACAGA GGGAAAATAA GATGAAAGAA ATTCAAAGAG GTGACAGTAC AGACAGCAGC 
AGGGACAAGC CTGTTCGTGT AACATCCCTA AAGCGAAAGC CATCTGAGCG GCCAGCATTG 
CCCCCTCGTC CCTTGTTGAC CAGTCCAGTC CTAAAGAACA GCGATTCACC CACACCTTCC 
CGAGTCCCTA CCCCCAGGTC TGCCTTGTGG GAAAGCAGAA GTGATAAAAC TGATTCTCCT 
GTGGTGTCAC GAGTGGCAGC AGGACTCAGA AGAAAGAGCA GCAGTGACAG TGATAGTGTA 
ACATCACCTG ACAAACCATC CACTTCTGGT CAGGATAGCC CTGACTCCTC CTCCACTGCG 
GTTTACAGAC CTCATTATCC ACACAATGCT TCTAAGGTTA AACATACCAC ATCTTCACAA 
GATGAAGTCG ATACTCTAAG AGAGACTGAA TCCTTTTTGA AAACCATTGA TAAACCAAAA 
ATCACTCCTA GGCGTCAGAA ACCATCAACT CCTGATAAAG AGTTGTGGGG TCCTCCTACA 
TCCAAACCAC CTGAGCCACC CATTAAACCT TCACAATCAT TGTCCAGATC TCCTCAGTCA 
CTGTCCAGAT CTCCTGAACC ACTGTCCAGA TCTCCAGAGC CATTGCCTAG ATCTCCTGAG 
CCACTGTCCA GATCTTCCCA GTCACAGCCC AGATCTCCTG AACCACTATC CAAATCGCCT 
GAACCACTAT CCAGATCTCC TGAGGCACTG TCCAGATCTC CCGAGCCACT GCCCAGATCT 
CCTCAGTCAC TGTCTAGATC TCCTGAGCCA CTGTCCAGAT CTCCTGAGTC ACCGTATATT 
CCTCCACTCA CAAAACCTCC ATCCACAAAC CCTCCTGATC CACCATCCAA GGCCACAGAA 
TTGGTGGACA ACCAAGACTC AAAAGATAGG AACCTTCCTC AATCCACTCC ACCATCAGTC 
CCTCAGTCAT CACCTCAATC TTTCGTTTCC AACCCAGAAC CACAAGCGTC CTCTTCTGTG 
CAATCCTCAA TAGTCTTTCC AGCTGCTTCC ACCATGATCA GAGCAGAAGA TGCCAAAATG 
GAGGGTACGT TGGAGATTAA ACTGAAGCAA GGAGGAAATA CAGGCTTGGA TCACTGGGAA 
ACGGTCTATG CACTACTGGA AGAGGAGACA CTCAATCTGT TTAAAGACCA AGATGCCGCC 
ACTGAGAACT GTACACGCTG GCCACCGATC AATTTGGCTG GAGCTATTTG TAAAGACAAC 
CCTTTTTACA GGAGAAAGGA GCACACGTTC AAACTCATGC TGAGTGATGG CAGTCACTAT 
CTGTTTGCTG CATCATCTCA AGGAGAACAG CAGAAGTGGC TGAAGGAGCT CCAGGAATGT 
ATTAATCCAA CTGTGTCTTC AGACAATCCT GGAATGAAGA GAGAATCAAG CAGTGAAAGC 
GTAGAGAAGA GCGAGTCTGT GCCAGTCGAC ACATCTGACA CACAAGACCA GGAGAAGGAA 
GACTCCACCG AGAGCTGTGA GACAGAGAGA GAACCACCAC CAAAACCTCC ACACACGTAC 
TACAACAAAC ACAAATACCC TGACGGAGGA GAGAGCCAAG ACTCAGTGAG TGTGTCTCAA 
AATATAAAAG GGCTACAGCC TCCCACATTC GCTCCTCCTC CTCCTCCAAC ACAAAACCAG 
ACAGAGAATG AAGCCAAGGC CAAAAACAGG AGCGGATTTA TGAAGTTTTT CAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207199.1
CDS	1..12360
Translation	MTVQKKTYTKWMNSVFSKSGEKVVLTDVFTELRTGVHLVRLLELISGEKLPTPSRRTLRVHCLENNSIAITFLKSKIRVDLIGPENVVDGDRTLILGLIWIIILRFQIGNINIVEAGGDASAARRSAKEALLIWCQRKTAGYTNVNVQDFSGSWRDGLAFNALIHAHRPDLFDYNRLRQDDPKRSLTHAFALAEDEFGIMQLLDVEDVMVPHPDEKSIMTYVSLYYHYFSKMKQGQTIQKRIAKIVDLLKELDDMKGLYESMVSDLLRWIKTKVVELNDRSFPNSLREMQHLVAAFKKYRTIEKPPKYQERGAIEAHLFDLRTKLRANNQRDYIPPEGKTLGDIERNWTLLERAEHQRERVLQSALMRLEQLEQLAQKFGRKATLRESYLEDTLQLIQQQDLGGLQRLEEAEIAARRLEALGADVLAHEPRFRALSEMAAVIERDNYHSKVQVIRRNVDIMNRWKDLKQVLQEQRAHVGDAANTFAVLRDIELISMDLKELQAQADSSDMGKQLPDVVALLQKQDLIDTQIISLGETLNAISSSSALKVKHKGATKVQRELKDLNSHYNSLLGLSKNRRKALEGQLKLFEFFHDCEEVEAWIYEKWVLLQAASLGRDLSQIQQAIQNHKALEAEIQSQESLCSGVVFRGQELCRGRHPNENDIQKWIRTLQKQWQQLKEQVTIRKNRLHAANVIKQYFADITEASSWLDDHKPLLIDEDYGKDEASSTALLQRHQRVEKEMEAYSSEVKRLGEQAKSAAQLAPLTTETQQTRMVHYSDSSDGEDEKEKKADKGGKAVSKILQQEQAMIRFKYRGPKLIWDRGEILTIISREKDDKLLLRDSRGNEQLVSSTYIRELPVSQEAPPETTNGVAESPKRKVSRPRRTRSMRRGTAEISTSTLPDPHFQKDTIESTQNSLEKDFNRLYNLAQSKNKILEDTARLHRFYNACEEFESWMGDKENVLNTFSSNSNNLEVVQAKYENFVTELASGRKQLDDITKFGDELLKQQHSRKKDIQQRLGIVTRRWERIQKLKDEMAHELLSSADVKSFLQTCQEVQAQLQHRLAQLDTPDVGSSPSALQAEEHRQAQAERDIEALERKIDYLKSVAKMKKDCSPAESAAIMEEVHALEDLLRQLKAQAIQRQRMLEEARRLQLFQKETRDLLMWAEATQEHLLEEETGSDVASAQALLKENLDLKQEIELQKAKLKDMERLGQSLDVASGEKGGKDVQQTLTKLDHEWSQLDKLWSSRNRRLEQGLEFQKLNMEADRIEATISGHEARLKVRDLGDSVDSVHSLLSRQEELEALLEALERSIILFQDKCQELISKRNFAAKEIQQRSKVIQDHFKRLKESCKLRRLELLASKKYQEFLRDAEEMLLWMDEKFEIAEDESYRDPTNILRKLKKHEAAEKEMQANAVRIDRLIETGEKMLADDHYNTQSIQKKTQEVHKRWIELQRKMAERGDKLRQAGQQEQLMELLQDAKLKIEAIERMLQNAIRGHDLRSSRQLLKEHKQLEEEAQELAEKINSIVTRAKNLATNHFDSQRILKETDTYLKLFKSLEKPLDHRRSQLEADVDLYSFYHDVDLELNWISEHQPVADTTNYERSLVGAVSLLQKHKDLQAEVNAHSKYLQRVLDRGRAMARSNQWNGQEVQQRCEQLIAKWEELEESCKKRSGELNRAVTREQILLDCTELETLVSETSALVSTDYGKTELATRSLIKQHEGVEGQIEVLAAQVDELKSNVKQAVRVWGLEELNKPCNHIKSKLSELQHSATVREQRLRETLHLHEFKRETSELEEWIAQQTQIAASNDLGSDYENAMQLRGRFEVFLKQLEVGKERMHTCEQLADKLIKSNHPESRFIRETQNLLRESWDELQDLSKDRKEKLRKSEKCHKFYKDLTDALGQIKERHKTIPEDIAKDLKGVQSQLRKHEALVHELAGTEQQLQELLDGTDAILGMCSPELRVGLQELQQEVVENWERLRMRMEQREEELQSAKDRYMFLNTAQDYSLWCSQVLSWMKAEESIRDVATCDLQLFQHQQLWAEIVAREETFAQAVSMGQGLLENDIPNAREIQEKLKALQEEREKLSDNWQLRQKWLENTYLEQVFYRDTEYMEKITNSQEIQLKNSDLGTTVDDVESLIKRHEAFEKLLHSQEDKMVALQESAERLKTEGLTREKNSNIKNKLKALQERRERIKDLSGKRREDLEVSKLHCIFSRDVSEAEEWITDRMNKIQDDSKMDMSNLQTKMKMLQKHQVFEAEILAHGNIIESVQQAGNELVTMHHPKSKEIRQTVSALISHWKALKQELAVRGKVLEDNRDFLEFLQKVEQVEVWIRQKEVMINVGDVGEDYEHGQQLLKKLSEFRGSGSGDVTVDDAHIKTINTLAARLERQNSDELVTVKKRRQQLNERWRNFHGNLSSYKRKLEAALEVHALIRELEEVRDRAGEKMLLLQGQGCGLDVESVENLIRRHEEMEREARVIQERGAALEKETKDRLKRHCDLSDKLSKKQEEVKIALVKLDKEIKRRKERLQESHQLQLFKANQRLLLDWTLKQSDEMVKKGLPKNKTEAESFILEHHDWKAEIDARGDRIDSVKSFGQNLVKSGHSDAAEIKEALRKLDDAKTMLTRAWEDRRKTLDQALKLQIFLGYADQTESWMSNREAFLINEDLGGSLSEVEALQRKLALFENSLEAQMEQVSEVDRYAQQLIQANHYDSDNIKKRTKAILLRKERILEISKSRRKALEESLQLQKFLEASYEISSWLNEKNSVAVDESWKDPINLQAKLLKHQSFEAEILANRNRVQAHIKDGDNMLAFSHPAKSKIKPRIKDVDDNWDQLLSNCKHKKTRLQQAYQALQFQRSLGDIEEWLGSVEVEVANDDYGGDLASVSRLLKALQGLEEMVDAHMEKVQGLVDTAKDFSSQGNFLADNIQQRVWEVVNRYNGLAEPMQTRRETLESWQLLFQFYRDLEDELLWIEDKLQATATKDWGTSLQSIQSIVKKHLVVMQEIENRTPLIMAVQEAGQNLVRGRHFASREISEKLSGLKKNFDALKKESANKDRLLQQALKIQSFLSEVSQLEQWMEEQKPVLESRDYGKNEEATEAFLRKLDTVDLELESQRGKVEALLKTGTDLEKSQHPNSHLVSKSLEDMQGEFETLLQLSGERRTELENQYNFYIFERDARDLQTWLLSRKTIAESDDYGKDLEGVEALQKKFEDFSEEVGTLGQSKLSTVQKLKQSVQSADAQQKEKDLLKLWEDLNKAMKARAENLHSAREVHQFEHDLDELRIWMNEKEVALDSEELKNDLLSVQALIRQHEGLERDLAVIEEDVSRRKEEGKALVRRSPRVRDSLSKRLQEVEEIWRNLLEKANQRRQRLQQAEAVQRYLIEWREMMGWLKETLSLMTGEGFGGKVKDLEQMIKKHDEYRTQIDRQLDKSEMVKNDGRRLMEEGNSWSHELEERLAELKDLEVRVLEAWTERRAQYQEDIELQQLQRELEQAEHWLNTYESALRAEDYGESVSDVLDLMKKQEDLEAMIQAQSERFNALQARRTQRENKMKEIQRGDSTDSSRDKPVRVTSLKRKPSERPALPPRPLLTSPVLKNSDSPTPSRVPTPRSALWESRSDKTDSPVVSRVAAGLRRKSSSDSDSVTSPDKPSTSGQDSPDSSSTAVYRPHYPHNASKVKHTTSSQDEVDTLRETESFLKTIDKPKITPRRQKPSTPDKELWGPPTSKPPEPPIKPSQSLSRSPQSLSRSPEPLSRSPEPLPRSPEPLSRSSQSQPRSPEPLSKSPEPLSRSPEALSRSPEPLPRSPQSLSRSPEPLSRSPESPYIPPLTKPPSTNPPDPPSKATELVDNQDSKDRNLPQSTPPSVPQSSPQSFVSNPEPQASSSVQSSIVFPAASTMIRAEDAKMEGTLEIKLKQGGNTGLDHWETVYALLEEETLNLFKDQDAATENCTRWPPINLAGAICKDNPFYRRKEHTFKLMLSDGSHYLFAASSQGEQQKWLKELQECINPTVSSDNPGMKRESSSESVEKSESVPVDTSDTQDQEKEDSTESCETEREPPPKPPHTYYNKHKYPDGGESQDSVSVSQNIKGLQPPTFAPPPPPTQNQTENEAKAKNRSGFMKFFRK

Target ORF information:

RefSeq Version	XM_017351710.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio spectrin, beta, non-erythrocytic 5 (sptbn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351710.1

ORF Insert Sequence:

ATGACGGTGC AGAAGAAGAC CTACACAAAA TGGATGAACA GCGTCTTCTC AAAGAGTGGG 
GAAAAGGTTG TCTTGACTGA TGTATTCACC GAATTAAGAA CTGGTGTGCA TCTCGTGCGA 
CTTCTGGAGC TCATTTCTGG AGAGAAGTTG CCCACTCCCA GTCGACGCAC ACTAAGAGTT 
CACTGTCTGG AAAACAACAG CATTGCCATC ACTTTCCTCA AATCAAAGAT CCGTGTAGAT 
CTGATTGGTC CAGAAAATGT GGTGGATGGA GACAGAACCC TGATCCTGGG TCTAATCTGG 
ATCATCATTC TCCGCTTCCA GATTGGGAAC ATCAATATAG TTGAGGCCGG CGGGGATGCC 
AGTGCGGCTC GGCGCTCAGC CAAAGAAGCT CTGCTCATCT GGTGTCAACG TAAGACTGCC 
GGTTACACCA ATGTAAATGT GCAAGACTTC TCAGGCAGCT GGAGAGATGG ACTGGCCTTC 
AATGCTCTAA TTCATGCCCA TCGGCCTGAT CTTTTTGACT ACAACCGACT CCGCCAGGAT 
GATCCAAAAA GGAGCCTCAC CCATGCTTTC GCTCTGGCAG AGGATGAATT TGGCATAATG 
CAGCTCCTCG ATGTTGAAGA TGTGATGGTG CCCCATCCTG ATGAGAAGTC CATCATGACT 
TACGTCTCTC TGTACTATCA CTACTTCTCA AAAATGAAGC AGGGGCAGAC TATCCAGAAG 
AGGATAGCTA AGATTGTGGA CCTTCTGAAA GAGCTGGATG ATATGAAGGG TCTGTATGAG 
AGTATGGTGT CTGACTTGCT ACGCTGGATC AAGACCAAGG TGGTGGAGCT CAATGACCGT 
TCCTTCCCCA ACTCCCTGCG AGAAATGCAG CATCTGGTAG CTGCCTTTAA GAAATACCGT 
ACTATTGAGA AGCCTCCCAA ATACCAGGAA CGGGGGGCCA TTGAGGCACA CCTCTTCGAC 
TTACGAACCA AGCTGAGGGC AAACAACCAG AGGGACTACA TTCCTCCGGA AGGCAAGACC 
TTGGGGGATA TTGAAAGGAA CTGGACCTTG CTAGAGCGAG CAGAACACCA GCGAGAACGG 
GTTCTCCAGA GTGCACTGAT GAGGCTGGAG CAGCTGGAGC AGTTGGCCCA GAAGTTTGGC 
CGTAAAGCGA CGCTGAGGGA GAGCTACCTG GAGGACACTC TGCAACTGAT TCAACAACAA 
GACCTGGGAG GACTGCAGAG GCTGGAGGAG GCTGAGATAG CAGCTCGCAG ACTGGAGGCC 
CTGGGAGCTG ATGTTTTGGC ACATGAACCA CGTTTCCGTG CTTTAAGTGA AATGGCTGCT 
GTCATCGAGA GGGATAACTA CCACAGCAAA GTACAAGTGA TTCGCAGAAA TGTGGACATC 
ATGAACCGCT GGAAGGATTT GAAGCAGGTT CTGCAGGAGC AGAGAGCACA TGTTGGAGAT 
GCGGCAAATA CATTTGCTGT CCTTAGAGAC ATTGAGTTAA TATCAATGGA CCTCAAGGAA 
TTACAGGCTC AGGCAGATTC CTCGGACATG GGAAAACAGC TGCCAGATGT GGTAGCCCTA 
CTGCAAAAGC AGGACTTGAT TGACACTCAG ATCATCTCAC TTGGTGAAAC TCTGAATGCC 
ATCAGTAGTA GCAGTGCCTT AAAAGTTAAA CACAAAGGTG CCACAAAAGT CCAGAGAGAA 
CTTAAAGATC TAAATAGCCA TTACAACTCC CTACTGGGTC TCAGCAAGAA CAGGAGAAAA 
GCTCTTGAGG GACAGCTGAA GCTCTTTGAG TTTTTCCATG ATTGTGAAGA AGTAGAAGCA 
TGGATCTATG AGAAATGGGT CCTCTTACAG GCAGCATCAC TAGGGAGAGA CCTCAGTCAA 
ATTCAGCAGG CCATTCAGAA CCACAAGGCT TTGGAGGCAG AGATTCAGTC ACAGGAATCT 
CTGTGCTCTG GGGTTGTATT TAGAGGACAA GAGCTTTGTA GAGGGAGACA CCCTAATGAG 
AATGACATCC AAAAGTGGAT TCGGACTCTT CAGAAACAGT GGCAGCAACT TAAAGAACAA 
GTCACCATCC GAAAGAACCG GCTGCATGCT GCCAATGTCA TCAAGCAGTA TTTTGCAGAC 
ATTACTGAAG CCAGCTCTTG GCTTGATGAC CACAAACCAC TTCTCATTGA CGAGGATTAT 
GGAAAGGATG AGGCCAGCTC TACTGCTCTG CTCCAGCGTC ACCAACGAGT GGAGAAAGAA 
ATGGAAGCAT ATTCCTCAGA AGTCAAACGA CTGGGTGAGC AGGCCAAGAG CGCTGCCCAA 
CTTGCTCCTC TTACAACTGA AACCCAGCAA ACGAGAATGG TCCACTACAG TGATTCATCA 
GATGGCGAAG ATGAGAAGGA GAAAAAAGCT GACAAAGGAG GCAAAGCAGT CAGCAAGATT 
TTACAACAAG AACAAGCAAT GATTAGGTTT AAATACAGAG GCCCTAAACT AATCTGGGAC 
AGAGGAGAAA TATTGACAAT TATCAGCAGG GAAAAGGATG ATAAGTTGCT TCTTCGGGAC 
AGCAGAGGAA ATGAGCAACT AGTCTCTTCC ACATACATCA GGGAGCTGCC AGTTTCTCAG 
GAAGCACCAC CTGAAACTAC AAACGGAGTA GCTGAGAGTC CAAAAAGAAA AGTGAGCCGC 
CCTCGCCGCA CTCGATCCAT GCGGCGTGGC ACAGCTGAAA TATCTACCTC AACCTTACCA 
GACCCACATT TCCAAAAAGA CACAATAGAG AGCACACAAA ACAGTCTGGA AAAGGATTTC 
AACCGCCTTT ACAATTTAGC ACAGTCAAAA AATAAGATCC TGGAGGACAC TGCACGACTA 
CACAGATTCT ACAATGCTTG TGAGGAGTTT GAGTCCTGGA TGGGAGACAA GGAGAATGTC 
CTCAACACTT TCAGCTCCAA TTCCAACAAC TTAGAGGTTG TACAAGCCAA ATATGAGAAT 
TTTGTAACTG AACTAGCCAG TGGTAGAAAG CAGCTTGATG ATATTACCAA GTTTGGAGAT 
GAGCTATTGA AACAACAGCA CAGCAGGAAA AAAGACATTC AGCAAAGACT TGGAATTGTT 
ACTCGCAGAT GGGAACGTAT TCAGAAACTA AAGGATGAGA TGGCTCATGA GCTTCTGAGT 
TCTGCTGATG TGAAGTCCTT CCTGCAGACC TGCCAGGAAG TCCAAGCTCA GCTCCAGCAC 
AGACTCGCCC AGCTTGACAC ACCTGATGTG GGGAGCTCAC CATCAGCCTT ACAAGCTGAG 
GAACACAGAC AAGCCCAAGC TGAAAGGGAC ATAGAAGCCC TTGAAAGGAA AATAGACTAT 
CTTAAAAGTG TGGCCAAGAT GAAAAAGGAC TGTAGTCCTG CAGAAAGTGC TGCTATCATG 
GAAGAAGTAC ATGCTCTGGA AGACTTGTTG CGGCAGTTAA AGGCTCAAGC CATTCAAAGA 
CAACGAATGC TTGAAGAAGC CAGACGCTTG CAACTCTTTC AAAAAGAGAC CAGAGACCTG 
TTGATGTGGG CTGAGGCAAC ACAGGAGCAT CTTCTGGAAG AGGAGACAGG ATCTGATGTA 
GCATCGGCTC AAGCCTTGTT GAAGGAGAAC CTTGATCTCA AGCAGGAGAT TGAACTGCAG 
AAAGCAAAAT TAAAAGATAT GGAGAGGCTG GGTCAGTCTC TTGACGTGGC ATCTGGAGAG 
AAAGGAGGTA AAGATGTTCA GCAGACACTT ACAAAGTTGG ATCATGAGTG GAGCCAGCTG 
GACAAACTGT GGTCTAGTCG CAACAGAAGG CTTGAACAAG GACTGGAGTT TCAGAAACTG 
AATATGGAAG CAGATCGTAT TGAGGCCACC ATTTCTGGTC ATGAAGCCAG ACTTAAAGTC 
AGAGACTTAG GGGACTCTGT GGACAGTGTT CACAGTTTAT TGAGCCGACA AGAAGAGCTT 
GAGGCTTTGC TTGAGGCTCT AGAGCGGTCT ATTATCCTCT TTCAAGACAA GTGCCAGGAG 
CTCATCAGCA AGCGTAACTT TGCTGCTAAA GAAATTCAAC AGCGATCAAA GGTCATTCAA 
GATCATTTCA AGAGGCTGAA AGAAAGTTGT AAATTAAGGA GGCTTGAATT ACTTGCTTCG 
AAAAAGTACC AGGAATTTTT AAGAGATGCT GAGGAGATGC TGTTGTGGAT GGATGAAAAG 
TTTGAGATCG CTGAGGATGA ATCGTACAGA GATCCAACCA ACATCCTTCG TAAGCTTAAA 
AAACATGAGG CAGCAGAAAA AGAGATGCAG GCCAATGCAG TTCGCATAGA CAGACTAATT 
GAGACTGGAG AGAAGATGTT GGCAGATGAT CACTACAACA CCCAGAGCAT TCAGAAAAAG 
ACTCAAGAGG TACACAAAAG GTGGATTGAA CTTCAGAGGA AGATGGCTGA AAGAGGGGAC 
AAGCTGCGAC AGGCTGGCCA ACAGGAGCAA CTCATGGAGC TGCTGCAGGA TGCTAAATTG 
AAGATTGAGG CAATTGAGAG AATGCTACAA AATGCAATCA GGGGTCATGA CCTCCGCTCC 
AGCAGACAGC TACTAAAAGA GCACAAGCAG CTGGAAGAGG AAGCTCAAGA ACTTGCTGAG 
AAAATCAATT CTATTGTCAC CCGTGCCAAA AACCTGGCCA CCAACCATTT TGACTCTCAG 
CGCATTCTCA AGGAGACTGA CACTTACCTG AAACTATTTA AGTCTCTAGA GAAACCTTTG 
GATCATCGCA GGAGCCAGTT AGAGGCAGAT GTGGATCTAT ACAGCTTTTA CCATGATGTA 
GACCTAGAGC TAAACTGGAT ATCAGAGCAT CAGCCTGTAG CTGATACTAC AAACTATGAG 
AGATCACTGG TTGGGGCAGT TAGCCTTCTT CAAAAACATA AGGACCTGCA GGCGGAGGTG 
AATGCCCACA GCAAGTACCT GCAGCGAGTA CTGGACAGAG GACGAGCCAT GGCCCGATCC 
AATCAGTGGA ATGGTCAGGA GGTGCAGCAG AGGTGTGAGC AATTGATCGC AAAGTGGGAA 
GAGTTAGAGG AATCCTGCAA GAAACGCTCC GGAGAACTTA ACAGGGCTGT TACACGGGAA 
CAGATTCTGC TTGACTGCAC TGAGCTGGAA ACCCTTGTGT CAGAAACATC AGCTTTAGTC 
AGCACTGATT ATGGCAAGAC TGAATTAGCT ACCCGCAGTC TAATAAAGCA ACACGAGGGA 
GTGGAGGGGC AAATCGAGGT ACTTGCTGCT CAAGTAGATG AACTCAAGTC CAATGTCAAG 
CAGGCTGTAC GTGTGTGGGG TTTAGAGGAG TTGAACAAGC CCTGCAACCA CATTAAATCT 
AAGCTGAGTG AGCTTCAACA CTCAGCGACA GTCAGAGAAC AGAGGCTTCG AGAGACTCTA 
CACCTACATG AATTTAAGCG TGAGACTAGT GAACTGGAGG AATGGATTGC TCAGCAGACA 
CAGATTGCAG CCTCTAATGA TCTTGGGAGT GATTATGAAA ATGCAATGCA ACTGCGTGGG 
AGATTTGAGG TCTTCTTGAA GCAGCTGGAG GTGGGAAAGG AGAGGATGCA CACCTGTGAA 
CAGTTGGCTG ACAAACTCAT CAAAAGCAAT CACCCTGAGA GTCGCTTCAT CCGAGAAACA 
CAGAATCTGC TCAGAGAATC GTGGGACGAG CTGCAGGATT TATCGAAAGA TCGTAAAGAA 
AAGCTTCGTA AGTCTGAAAA ATGTCACAAA TTCTACAAAG ACCTGACCGA TGCACTGGGA 
CAGATCAAGG AGAGGCACAA AACCATTCCT GAAGACATAG CCAAAGATCT GAAAGGAGTG 
CAGTCACAGC TACGCAAACA TGAAGCTCTT GTGCACGAGC TGGCTGGAAC TGAGCAACAG 
CTGCAGGAGC TGTTGGATGG AACCGATGCT ATTTTGGGCA TGTGCTCTCC TGAGCTGCGG 
GTGGGGTTGC AGGAACTACA ACAGGAGGTG GTGGAGAACT GGGAGCGGCT CCGCATGCGG 
ATGGAGCAGA GAGAAGAGGA GCTGCAATCG GCTAAAGACC GCTACATGTT CCTCAACACG 
GCACAGGACT ACTCCCTGTG GTGCTCTCAG GTGCTGAGCT GGATGAAGGC AGAGGAGTCC 
ATTCGAGACG TTGCCACCTG TGACCTGCAG TTGTTCCAGC ACCAGCAGTT GTGGGCTGAG 
ATTGTGGCCC GTGAGGAGAC GTTTGCCCAG GCTGTGTCAA TGGGTCAAGG CCTTCTGGAG 
AATGACATAC CTAATGCTAG AGAGATCCAG GAGAAACTGA AAGCTCTGCA AGAAGAGAGA 
GAGAAGCTTT CCGATAACTG GCAGCTCAGG CAGAAATGGC TGGAGAACAC ATATCTGGAG 
CAGGTGTTTT ACAGAGACAC TGAGTACATG GAGAAGATCA CCAACTCTCA AGAGATCCAG 
TTGAAGAACA GTGATTTAGG AACGACAGTG GATGATGTTG AAAGTTTGAT TAAGCGCCAT 
GAGGCCTTTG AAAAACTTCT TCATTCTCAG GAAGATAAGA TGGTGGCTCT TCAGGAATCA 
GCAGAGCGTT TAAAGACAGA AGGTTTAACA CGAGAAAAAA ACAGCAATAT TAAGAACAAA 
CTCAAAGCTC TTCAGGAAAG GAGGGAACGG ATTAAAGATC TGTCTGGCAA ACGAAGAGAG 
GATCTTGAGG TCTCAAAGCT TCACTGTATT TTCAGCCGAG ATGTGTCTGA GGCAGAGGAA 
TGGATCACAG ATCGTATGAA CAAGATACAA GATGACTCCA AAATGGACAT GAGCAACCTT 
CAGACTAAAA TGAAGATGCT GCAGAAGCAT CAGGTGTTTG AAGCTGAGAT TCTGGCCCAT 
GGAAATATCA TTGAATCTGT CCAGCAGGCT GGAAATGAGC TGGTGACCAT GCATCACCCA 
AAATCAAAGG AGATACGCCA GACTGTTTCA GCGCTCATCT CTCATTGGAA GGCTCTAAAG 
CAAGAGCTTG CGGTGCGTGG AAAGGTTCTA GAGGATAACC GTGATTTTCT TGAGTTCCTG 
CAGAAAGTGG AACAGGTTGA AGTTTGGATT AGGCAAAAGG AGGTGATGAT AAATGTTGGA 
GATGTCGGGG AGGATTATGA ACACGGTCAG CAGCTGCTGA AGAAGCTCAG TGAATTCAGA 
GGGTCAGGCT CTGGAGATGT GACTGTTGAC GATGCTCACA TCAAGACAAT CAACACTTTG 
GCCGCTCGAC TTGAGAGACA GAACAGTGAT GAGCTGGTGA CTGTGAAAAA GCGTAGGCAG 
CAGCTCAATG AAAGATGGAG GAATTTCCAT GGCAACCTGA GCAGCTATAA GAGGAAGCTG 
GAGGCAGCGT TGGAGGTCCA CGCATTGATC CGAGAACTAG AGGAGGTCCG GGACAGAGCT 
GGAGAGAAGA TGCTGTTGCT CCAGGGTCAG GGTTGCGGGT TGGATGTTGA AAGTGTGGAG 
AACCTCATTC GCAGACATGA AGAGATGGAG AGGGAGGCCA GGGTCATCCA GGAGAGAGGC 
GCTGCCCTGG AAAAGGAGAC TAAAGATCGG TTGAAGAGAC ACTGCGATCT GTCTGACAAA 
CTTAGCAAGA AGCAGGAAGA GGTCAAAATT GCTTTAGTCA AGCTGGACAA GGAAATCAAA 
CGAAGGAAGG AGCGATTGCA GGAGTCTCAT CAGCTCCAGC TGTTTAAAGC GAATCAGCGT 
CTGCTTTTGG ACTGGACCCT CAAGCAAAGT GATGAGATGG TGAAGAAAGG CCTGCCTAAG 
AATAAAACCG AAGCTGAGAG TTTCATATTA GAACATCACG ACTGGAAGGC TGAAATTGAT 
GCTCGTGGCG ATCGGATAGA TTCAGTTAAG AGTTTCGGAC AGAATCTTGT GAAGTCTGGA 
CACAGTGATG CAGCAGAGAT TAAAGAAGCC CTACGCAAGC TGGATGATGC CAAGACAATG 
CTTACCCGAG CTTGGGAGGA CCGAAGAAAA ACTCTGGATC AAGCCCTGAA GCTCCAGATA 
TTCCTGGGCT ATGCTGACCA GACTGAGAGC TGGATGAGTA ACAGGGAGGC TTTCTTGATC 
AATGAAGATC TAGGGGGTTC ATTGTCGGAG GTGGAGGCTC TACAGAGGAA GCTGGCTCTG 
TTTGAGAACT CTCTAGAAGC TCAGATGGAG CAGGTTTCAG AGGTTGACAG ATACGCTCAG 
CAGCTCATAC AGGCAAACCA CTACGACTCT GACAACATCA AGAAGAGGAC AAAAGCTATT 
CTGCTCAGGA AGGAAAGAAT ACTGGAAATC AGCAAATCCC GGCGAAAAGC TCTAGAAGAA 
TCTCTTCAGT TGCAAAAGTT CCTGGAAGCA AGTTATGAGA TTTCCTCATG GCTGAATGAG 
AAGAACTCAG TGGCTGTTGA CGAGAGTTGG AAAGATCCTA TAAATCTGCA GGCTAAGCTA 
CTGAAACACC AGAGCTTTGA AGCAGAGATT CTGGCCAACC GCAACAGAGT GCAGGCACAC 
ATTAAAGATG GTGATAACAT GCTGGCTTTC AGTCATCCTG CTAAAAGCAA AATAAAACCA 
CGAATAAAGG ATGTCGATGA CAACTGGGAC CAGCTTTTAA GCAACTGCAA GCATAAGAAG 
ACACGTCTAC AGCAAGCGTA TCAGGCCTTA CAGTTCCAGC GTTCATTAGG TGACATTGAG 
GAGTGGCTTG GGTCGGTGGA GGTGGAAGTG GCGAATGACG ACTATGGAGG TGATCTGGCC 
TCTGTTAGCA GGCTGCTTAA GGCTCTACAG GGTCTGGAGG AAATGGTAGA CGCGCACATG 
GAGAAAGTCC AGGGGCTAGT GGACACTGCC AAAGACTTCA GCTCTCAGGG CAACTTCCTC 
GCAGATAATA TACAGCAGAG GGTTTGGGAG GTGGTCAACA GGTATAATGG CCTGGCTGAG 
CCGATGCAGA CCCGCAGGGA GACTCTGGAG TCGTGGCAGC TGCTGTTCCA GTTCTACAGG 
GACTTAGAGG ATGAGCTGCT GTGGATTGAG GACAAGCTGC AGGCCACGGC CACCAAAGAC 
TGGGGCACCT CCCTACAGAG CATACAATCT ATAGTCAAAA AACATCTGGT TGTGATGCAG 
GAAATTGAGA ACCGAACTCC TCTAATAATG GCTGTACAGG AGGCAGGACA GAATTTGGTG 
CGAGGACGAC ACTTTGCATC ACGTGAGATC AGTGAAAAAC TTTCAGGGTT AAAGAAGAAC 
TTTGACGCCC TGAAGAAGGA GTCAGCGAAT AAAGATCGCC TGCTTCAACA AGCACTGAAA 
ATCCAGAGCT TTCTCTCAGA GGTTTCCCAG TTGGAGCAGT GGATGGAGGA ACAGAAGCCT 
GTTTTAGAGT CCCGCGACTA TGGAAAGAAT GAGGAGGCCA CTGAAGCTTT TCTCAGGAAG 
CTGGACACAG TTGACCTGGA GTTGGAAAGC CAGCGTGGAA AAGTAGAGGC TCTCCTGAAA 
ACCGGCACAG ATTTAGAGAA GTCTCAGCAT CCCAACAGCC ATTTAGTGAG TAAGAGCCTT 
GAAGATATGC AGGGTGAGTT TGAGACACTA CTGCAACTGT CTGGTGAACG TCGCACAGAG 
CTGGAGAATC AGTACAATTT CTACATATTT GAGAGAGATG CCAGAGACCT ACAGACCTGG 
CTCCTTTCTC GGAAGACCAT AGCAGAATCT GATGATTATG GAAAAGATCT GGAAGGAGTG 
GAAGCACTGC AAAAGAAGTT TGAGGATTTT TCAGAAGAGG TTGGGACTCT GGGCCAGAGT 
AAGCTGAGCA CTGTTCAAAA GCTAAAGCAA AGTGTGCAGT CAGCAGATGC ACAACAAAAA 
GAGAAAGATC TACTCAAGCT CTGGGAGGAT TTAAACAAGG CTATGAAAGC AAGAGCAGAG 
AACCTGCATT CAGCTCGAGA AGTTCACCAG TTTGAACATG ACCTGGATGA GCTTCGGATC 
TGGATGAATG AGAAGGAAGT TGCTCTGGAC TCTGAGGAGC TGAAGAATGA CCTGCTTAGT 
GTCCAAGCTC TCATCCGTCA ACATGAAGGA CTGGAGAGAG ACCTTGCAGT GATAGAAGAA 
GATGTGTCTC GTCGAAAAGA GGAGGGAAAA GCTTTGGTCC GCAGGTCACC TCGAGTAAGG 
GACAGTTTGA GCAAGAGACT CCAGGAAGTG GAGGAGATCT GGAGAAACCT GCTGGAGAAA 
GCCAACCAGC GCAGACAAAG ACTCCAACAG GCAGAGGCCG TTCAAAGATA TCTGATTGAA 
TGGAGAGAGA TGATGGGCTG GCTGAAAGAG ACACTGTCTC TGATGACAGG GGAGGGATTC 
GGAGGCAAGG TTAAAGACCT GGAACAGATG ATTAAAAAAC ATGATGAGTA CCGCACACAG 
ATTGACAGAC AACTGGACAA ATCGGAAATG GTTAAAAATG ATGGGAGACG TCTGATGGAG 
GAGGGGAACT CATGGAGTCA TGAGCTTGAG GAGCGTCTAG CCGAGCTCAA GGATCTGGAG 
GTGCGGGTGC TGGAGGCCTG GACTGAAAGG AGAGCTCAGT ATCAGGAAGA CATAGAGCTC 
CAGCAACTGC AGAGGGAACT GGAGCAGGCC GAGCACTGGC TCAACACTTA TGAGAGCGCG 
CTCAGAGCAG AGGACTATGG GGAATCAGTT TCAGATGTTC TGGATTTGAT GAAAAAGCAG 
GAAGATCTGG AGGCCATGAT TCAAGCACAG AGTGAGAGAT TCAATGCCCT TCAAGCCAGA 
AGAACACAGA GGGAAAATAA GATGAAAGAA ATTCAAAGAG GTGACAGTAC AGACAGCAGC 
AGGGACAAGC CTGTTCGTGT AACATCCCTA AAGCGAAAGC CATCTGAGCG GCCAGCATTG 
CCCCCTCGTC CCTTGTTGAC CAGTCCAGTC CTAAAGAACA GCGATTCACC CACACCTTCC 
CGAGTCCCTA CCCCCAGGTC TGCCTTGTGG GAAAGCAGAA GTGATAAAAC TGATTCTCCT 
GTGGTGTCAC GAGTGGCAGC AGGACTCAGA AGAAAGAGCA GCAGTGACAG TGATAGTGTA 
ACATCACCTG ACAAACCATC CACTTCTGGT CAGGATAGCC CTGACTCCTC CTCCACTGCG 
GTTTACAGAC CTCATTATCC ACACAATGCT TCTAAGGTTA AACATACCAC ATCTTCACAA 
GATGAAGTCG ATACTCTAAG AGAGACTGAA TCCTTTTTGA AAACCATTGA TAAACCAAAA 
ATCACTCCTA GGCGTCAGAA ACCATCAACT CCTGATAAAG AGTTGTGGGG TCCTCCTACA 
TCCAAACCAC CTGAGCCACC CATTAAACCT TCACAATCAT TGTCCAGATC TCCTCAGTCA 
CTGTCCAGAT CTCCTGAACC ACTGTCCAGA TCTCCAGAGC CATTGCCTAG ATCTCCTGAG 
CCACTGTCCA GATCTTCCCA GTCACAGCCC AGATCTCCTG AACCACTATC CAAATCGCCT 
GAACCACTAT CCAGATCTCC TGAGGCACTG TCCAGATCTC CCGAGCCACT GCCCAGATCT 
CCTCAGTCAC TGTCTAGATC TCCTGAGCCA CTGTCCAGAT CTCCTGAGTC ACCGTATATT 
CCTCCACTCA CAAAACCTCC ATCCACAAAC CCTCCTGATC CACCATCCAA GGCCACAGAA 
TTGGTGGACA ACCAAGACTC AAAAGATAGG AACCTTCCTC AATCCACTCC ACCATCAGTC 
CCTCAGTCAT CACCTCAATC TTTCGTTTCC AACCCAGAAC CACAAGCGTC CTCTTCTGTG 
CAATCCTCAA TAGTCTTTCC AGCTGCTTCC ACCATGATCA GAGCAGAAGA TGCCAAAATG 
GAGGGTACGT TGGAGATTAA ACTGAAGCAA GGAGGAAATA CAGGCTTGGA TCACTGGGAA 
ACGGTCTATG CACTACTGGA AGAGGAGACA CTCAATCTGT TTAAAGACCA AGATGCCGCC 
ACTGAGAACT GTACACGCTG GCCACCGATC AATTTGGCTG GAGCTATTTG TAAAGACAAC 
CCTTTTTACA GGAGAAAGGA GCACACGTTC AAACTCATGC TGAGTGATGG CAGTCACTAT 
CTGTTTGCTG CATCATCTCA AGGAGAACAG CAGAAGTGGC TGAAGGAGCT CCAGGAATGT 
ATTAATCCAA CTGTGTCTTC AGACAATCCT GGAATGAAGA GAGAATCAAG CAGTGAAAGC 
GTAGAGAAGA GCGAGTCTGT GCCAGTCGAC ACATCTGACA CACAAGACCA GGAGAAGGAA 
GACTCCACCG AGAGCTGTGA GACAGAGAGA GAACCACCAC CAAAACCTCC ACACACGTAC 
TACAACAAAC ACAAATACCC TGACGGAGGA GAGAGCCAAG ACTCAGTGAG TGTGTCTCAA 
AATATAAAAG GGCTACAGCC TCCCACATTC GCTCCTCCTC CTCCTCCAAC ACAAAACCAG 
ACAGAGAATG AAGCCAAGGC CAAAAACAGG AGCGGATTTA TGAAGTTTTT CAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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sptbn5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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