Cacna1h cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1h
Entrez Gene ID	58226
Full Name	calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms	Cav3.2,MNCb-1209,alpha13.2
General protein information	Preferred Names calcium channel, voltage-dependent, T type, alpha 1H subunit Names voltage-dependent T-type calcium channel subunit alpha-1H T-type Cav3.2 calcium channel alpha13.2 subunit low-voltage-activated calcium channel alpha13.2 subunit voltage-gated calcium channel subunit alpha Cav3.2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 12.53 cM
Summary	Voltage-dependent Ca(2+) channels mediate the entry of Ca(2+) ions into excitable cells and are involved in a variety of Ca(2+)-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. The protein encoded by this gene is an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246555.2	XP_011244857.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
NM_001163691.1	NP_001157163.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform 2
XM_011246555.3	XP_011244857.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
XM_006524734.3	XP_006524797.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
XM_006524734.4	XP_006524797.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
XM_006524733.4	XP_006524796.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
XM_006524733.3	XP_006524796.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
NM_021415.4	NP_067390.4	voltage-dependent T-type calcium channel subunit alpha-1H isoform 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC560875	XP_003198197.2
Homo sapiens (human)	CACNA1H	NP_066921.2
Bos taurus (cattle)	CACNA1H	XP_002698020.3
Mus musculus (house mouse)	Cacna1h	NP_067390.4
Rattus norvegicus (Norway rat)	Cacna1h	NP_722521.1
Gallus gallus (chicken)	CACNA1H	XP_414830.4
Xenopus tropicalis (tropical clawed frog)	LOC100494894	XP_002932520.2
Pan troglodytes (chimpanzee)	CACNA1H	XP_001172468.2
Canis lupus familiaris (dog)	CACNA1H	XP_537016.2

Gene Ontology
Bibliography

Related articles in PubMed

The T-type Ca
Rebellato P, Kaczynska D, Kanatani S, Rayyes IA, Zhang S, Villaescusa C, Falk A, Arenas E, Hermanson O, Louhivuori L, Uhlén P
Neuroscience40278-89(2019 03)

Cav3.2 calcium channel interactions with the epithelial sodium channel ENaC.
Garcia-Caballero A, Gandini MA, Huang S, Chen L, Souza IA, Dang YL, Stutts MJ, Zamponi GW
Molecular brain12(1)12(2019 02)

Cav3.2 T-type calcium channels shape electrical firing in mouse Lamina II neurons.
Candelas M, Reynders A, Arango-Lievano M, Neumayer C, Fruquière A, Demes E, Hamid J, Lemmers C, Bernat C, Monteil A, Compan V, Laffray S, Inquimbert P, Le Feuvre Y, Zamponi GW, Moqrich A, Bourinet E, Méry PF
Scientific reports9(1)3112(2019 Feb)

Caveolae Link Ca
Hashad AM, Harraz OF, Brett SE, Romero M, Kassmann M, Puglisi JL, Wilson SM, Gollasch M, Welsh DG
Arteriosclerosis, thrombosis, and vascular biology38(10)2371-2381(2018 10)

Disrupting USP5/Cav3.2 interactions protects female mice from mechanical hypersensitivity during peripheral inflammation.
Gadotti VM, Zamponi GW
Molecular brain11(1)60(2018 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cacna1h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69781	XM_011246555.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu16606	NM_021415.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu16623	NM_001163691.1 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu53886	XM_006524733.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu53887	XM_006524734.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OMu69781	XM_011246555.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu53887	XM_006524734.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu53886	XM_006524733.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1h ( XM_011246555.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244857.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu69781
Clone ID Related Accession (Same CDS sequence)	XM_011246555.2 , XM_011246555.3
Accession Version	XM_011246555.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246555.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC TTCTTCGTGC TCAACATGTT 
TGTGGGCGTG GTGGTGGAGA ACTTCCACAA GTGCCGGCAG CACCAGGAGG CGGAGGAAGC 
GCGGCGGCGC GAGGAGAAAC GGCTGCGGCG CCTGGAGAGG AGGCGCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244857.1
CDS	326..5059
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQLLRAQHVCGRGGGELPQVPAAPGGGGSAAARGETAAAPGEEAQE

Target ORF information:

RefSeq Version	XM_011246555.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246555.2

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC TTCTTCGTGC TCAACATGTT 
TGTGGGCGTG GTGGTGGAGA ACTTCCACAA GTGCCGGCAG CACCAGGAGG CGGAGGAAGC 
GCGGCGGCGC GAGGAGAAAC GGCTGCGGCG CCTGGAGAGG AGGCGCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1h ( XM_011246555.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244857.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X5, mRNA.

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CloneID	OMu69781
Clone ID Related Accession (Same CDS sequence)	XM_011246555.2 , XM_011246555.3
Accession Version	XM_011246555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC TTCTTCGTGC TCAACATGTT 
TGTGGGCGTG GTGGTGGAGA ACTTCCACAA GTGCCGGCAG CACCAGGAGG CGGAGGAAGC 
GCGGCGGCGC GAGGAGAAAC GGCTGCGGCG CCTGGAGAGG AGGCGCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244857.1
CDS	2884..7617
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQLLRAQHVCGRGGGELPQVPAAPGGGGSAAARGETAAAPGEEAQE

Target ORF information:

RefSeq Version	XM_011246555.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246555.3

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC TTCTTCGTGC TCAACATGTT 
TGTGGGCGTG GTGGTGGAGA ACTTCCACAA GTGCCGGCAG CACCAGGAGG CGGAGGAAGC 
GCGGCGGCGC GAGGAGAAAC GGCTGCGGCG CCTGGAGAGG AGGCGCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1h ( NM_021415.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067390.4 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16606
Clone ID Related Accession (Same CDS sequence)	NM_021415.4
Accession Version	NM_021415.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK147242.1, AK135951.1, AC122454.4 and AI834839.1. On Jul 29, 2009 this sequence version replaced NM_021415.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138026.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAAGGCCCAG 
CGCCGGCCCT ACTACGCAGA CTATTCACAC ACGCGCCGCT CCATCCATTC GCTGTGCACC 
AGCCACTACC TGGACCTCTT CATCACCTTC ATCATCTGCC TCAATGTCAT CACCATGTCC 
ATGGAGCACT ACAACCAGCC CAAGTCTCTG GACGAGGCCC TTAAGTACTG CAACTACGTC 
TTTACCATCG TCTTCGTCTT TGAGGCCGCA CTGAAGCTGG TGGCCTTTGG ATTCCGGAGG 
TTCTTCAAGG ACAGGTGGAA CCAGCTGGAC TTGGCCATCG TCCTCCTGTC CATCATGGGC 
ATTGCGCTGG AGGAGATTGA GATGAATGCT GCCCTCCCCA TCAACCCCAC CATCATCCGC 
ATCATGCGCG TGCTTCGCAT CGCCCGTGTG CTGAAGCTAC TGAAGATGGC CACAGGCATG 
CGCGCCCTGC TGGACACTGT GGTTCAAGCT CTGCCTCAGG TAGGGAACCT TGGTCTTCTT 
TTCATGCTCC TGTTTTTTAT CTATGCTGCC CTGGGAGTGG AGCTGTTTGG GAGGCTAGAA 
TGCAGCGAGG ATAATCCCTG TGAGGGCCTG AGCAGACACG CCACCTTCAC CAACTTTGGC 
ATGGCCTTCC TCACGTTGTT CCGAGTGTCC ACTGGGGACA ACTGGAATGG GATCATGAAG 
GATACTCTCC GTGAATGTAC CCGTGAAGAC AAGCACTGCC TCAGCTACCT GCCCGCGCTC 
TCCCCCGTCT ACTTCGTCAC CTTCGTGCTG GTCGCCCAGT TCGTGCTGGT CAACGTGGTG 
GTGGCCGTGC TCATGAAGCA CCTGGAGGAG AGCAACAAGG AGGCCCGGGA GGATGCGGAG 
ATGGATGCCG AGATCGAGCT GGAGATCGCA CAGGGGTCCA CAGCCCAGCC CCCGTCCACA 
GCACAGGAAA GCCAAGGTAC CGAGCCAGAC ACCCCGAACC TCCTGGTCGT GCGCAAAGTA 
TCTGTGTCCA GGATGCTCTC GCTACCCAAT GACAGCTACA TGTTCCGGCC GGTGGCTCCT 
GCGGCTGCCC CGCATTCCCA CCCACTGCAG GAAGTGGAGA TGGAGACCTA CACAGGCCCG 
GTCACCTCTG CTCACTCGCC ATCCCTGGAG CCCCGCACCT CCTTCCAGGT CCCATCAGCT 
GCGTCCTCCC CAGCCAGGGT CAGCGACCCC CTTTGTGCCC TCTCACCCCG GGATACGCCC 
CGCTCTCTGA GCCTCTCACG GATACTCTGC AGACAGGAGG CCATGCACGC AGAGTCCCTG 
GAAGGGCAGA TAGATGACGC TGGAGAAGAC AGCATCCCAG ACTACACGGA GCCTGCTGAA 
AATATCTCCA TGAGCCAGGC ACCTCTGGGT ACCCTGCGGT CTCCTCCATG CTCCCCACGG 
CCTGCCAGTG TCCGTACCCG CAAGCACACT TTTGGGCAGC ACTGCATCTC CAGCCGCCCT 
CCCACCCTGG GAGGAGATGA TGCTGAAGCA GCAGACCCGG CCGATGAGGA GGTCAGCCAC 
ATCACCAGCT CAGCCCACCC TTGGCCAGCT ACAGAGCCCC ACAGCCCAGA GGCCTCCCCG 
ACAGCCTCTC CTGCAAAAGG CACAGTGGGC AGTGGGCGGG ACCCACACAG GTTCTGCAGT 
GTAGATGCTC AGAGCTTCCT GGACAAACCA GGTCGGCCAG ATGCACAGCG GTGGTCCTCG 
GTGGAACTGG ATAATGGGGA TGGCCACCTA GAGTCTGGGG AAGTGAGGGC CCGGGCCTCA 
GAGCTAGAAC CAGCTCTTGG GGCACGGAGG AAGAAGAAGA TGAGCCCTCC CTGCATCTCC 
ATTGACCCTC CCACTGAAGA TGAGGGCTCC TCCCGCCCCC CTGCAGCAGA AGGAGGCAAC 
ACTACTCTGA GGCGCCGAAC CCCGTCCTGT GAGGCTGCCC TCCATAGGGA CTGCCCAGAG 
TCTACAGAGG GCCCAGGCAC TGGAGGGGAC CCTGTAGCCA AGGGTGAGCG CTGGGGCCAG 
GCCTCTTGCC GGGCAGAGCA TCTGACTGTC CCCAACTTTG CCTTTGAGCC TCTGGACATG 
GGCGGACCTG GTGGAGACTG TTTCTTGGAC AGTGACCAAA GTGTGACCCC AGAACCCAGA 
GTTTCCTCTT TGGGGGCTAT AGTGCCTCTA ATACTAGAAA CTGAACTTTC TATGCCCTCT 
GGTGACCCCC CAGAGAAGGA GCAAGGACTG TACCTCACTG TGCCCCAGAC CCCCTTGAAG 
AAACCAGGGT CTCCCCCAGC CACTCCTGCC CCAGATGACA GTGGAGATGA GCCTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067390.4
CDS	333..7412
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQGTEPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGPVTSAHSPSLEPRTSFQVPSAASSPARVSDPLCALSPRDTPRSLSLSRILCRQEAMHAESLEGQIDDAGEDSIPDYTEPAENISMSQAPLGTLRSPPCSPRPASVRTRKHTFGQHCISSRPPTLGGDDAEAADPADEEVSHITSSAHPWPATEPHSPEASPTASPAKGTVGSGRDPHRFCSVDAQSFLDKPGRPDAQRWSSVELDNGDGHLESGEVRARASELEPALGARRKKKMSPPCISIDPPTEDEGSSRPPAAEGGNTTLRRRTPSCEAALHRDCPESTEGPGTGGDPVAKGERWGQASCRAEHLTVPNFAFEPLDMGGPGGDCFLDSDQSVTPEPRVSSLGAIVPLILETELSMPSGDPPEKEQGLYLTVPQTPLKKPGSPPATPAPDDSGDEPV

Target ORF information:

RefSeq Version	NM_021415.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021415.4

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAAGGCCCAG 
CGCCGGCCCT ACTACGCAGA CTATTCACAC ACGCGCCGCT CCATCCATTC GCTGTGCACC 
AGCCACTACC TGGACCTCTT CATCACCTTC ATCATCTGCC TCAATGTCAT CACCATGTCC 
ATGGAGCACT ACAACCAGCC CAAGTCTCTG GACGAGGCCC TTAAGTACTG CAACTACGTC 
TTTACCATCG TCTTCGTCTT TGAGGCCGCA CTGAAGCTGG TGGCCTTTGG ATTCCGGAGG 
TTCTTCAAGG ACAGGTGGAA CCAGCTGGAC TTGGCCATCG TCCTCCTGTC CATCATGGGC 
ATTGCGCTGG AGGAGATTGA GATGAATGCT GCCCTCCCCA TCAACCCCAC CATCATCCGC 
ATCATGCGCG TGCTTCGCAT CGCCCGTGTG CTGAAGCTAC TGAAGATGGC CACAGGCATG 
CGCGCCCTGC TGGACACTGT GGTTCAAGCT CTGCCTCAGG TAGGGAACCT TGGTCTTCTT 
TTCATGCTCC TGTTTTTTAT CTATGCTGCC CTGGGAGTGG AGCTGTTTGG GAGGCTAGAA 
TGCAGCGAGG ATAATCCCTG TGAGGGCCTG AGCAGACACG CCACCTTCAC CAACTTTGGC 
ATGGCCTTCC TCACGTTGTT CCGAGTGTCC ACTGGGGACA ACTGGAATGG GATCATGAAG 
GATACTCTCC GTGAATGTAC CCGTGAAGAC AAGCACTGCC TCAGCTACCT GCCCGCGCTC 
TCCCCCGTCT ACTTCGTCAC CTTCGTGCTG GTCGCCCAGT TCGTGCTGGT CAACGTGGTG 
GTGGCCGTGC TCATGAAGCA CCTGGAGGAG AGCAACAAGG AGGCCCGGGA GGATGCGGAG 
ATGGATGCCG AGATCGAGCT GGAGATCGCA CAGGGGTCCA CAGCCCAGCC CCCGTCCACA 
GCACAGGAAA GCCAAGGTAC CGAGCCAGAC ACCCCGAACC TCCTGGTCGT GCGCAAAGTA 
TCTGTGTCCA GGATGCTCTC GCTACCCAAT GACAGCTACA TGTTCCGGCC GGTGGCTCCT 
GCGGCTGCCC CGCATTCCCA CCCACTGCAG GAAGTGGAGA TGGAGACCTA CACAGGCCCG 
GTCACCTCTG CTCACTCGCC ATCCCTGGAG CCCCGCACCT CCTTCCAGGT CCCATCAGCT 
GCGTCCTCCC CAGCCAGGGT CAGCGACCCC CTTTGTGCCC TCTCACCCCG GGATACGCCC 
CGCTCTCTGA GCCTCTCACG GATACTCTGC AGACAGGAGG CCATGCACGC AGAGTCCCTG 
GAAGGGCAGA TAGATGACGC TGGAGAAGAC AGCATCCCAG ACTACACGGA GCCTGCTGAA 
AATATCTCCA TGAGCCAGGC ACCTCTGGGT ACCCTGCGGT CTCCTCCATG CTCCCCACGG 
CCTGCCAGTG TCCGTACCCG CAAGCACACT TTTGGGCAGC ACTGCATCTC CAGCCGCCCT 
CCCACCCTGG GAGGAGATGA TGCTGAAGCA GCAGACCCGG CCGATGAGGA GGTCAGCCAC 
ATCACCAGCT CAGCCCACCC TTGGCCAGCT ACAGAGCCCC ACAGCCCAGA GGCCTCCCCG 
ACAGCCTCTC CTGCAAAAGG CACAGTGGGC AGTGGGCGGG ACCCACACAG GTTCTGCAGT 
GTAGATGCTC AGAGCTTCCT GGACAAACCA GGTCGGCCAG ATGCACAGCG GTGGTCCTCG 
GTGGAACTGG ATAATGGGGA TGGCCACCTA GAGTCTGGGG AAGTGAGGGC CCGGGCCTCA 
GAGCTAGAAC CAGCTCTTGG GGCACGGAGG AAGAAGAAGA TGAGCCCTCC CTGCATCTCC 
ATTGACCCTC CCACTGAAGA TGAGGGCTCC TCCCGCCCCC CTGCAGCAGA AGGAGGCAAC 
ACTACTCTGA GGCGCCGAAC CCCGTCCTGT GAGGCTGCCC TCCATAGGGA CTGCCCAGAG 
TCTACAGAGG GCCCAGGCAC TGGAGGGGAC CCTGTAGCCA AGGGTGAGCG CTGGGGCCAG 
GCCTCTTGCC GGGCAGAGCA TCTGACTGTC CCCAACTTTG CCTTTGAGCC TCTGGACATG 
GGCGGACCTG GTGGAGACTG TTTCTTGGAC AGTGACCAAA GTGTGACCCC AGAACCCAGA 
GTTTCCTCTT TGGGGGCTAT AGTGCCTCTA ATACTAGAAA CTGAACTTTC TATGCCCTCT 
GGTGACCCCC CAGAGAAGGA GCAAGGACTG TACCTCACTG TGCCCCAGAC CCCCTTGAAG 
AAACCAGGGT CTCCCCCAGC CACTCCTGCC CCAGATGACA GTGGAGATGA GCCTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1h ( NM_001163691.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157163.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant 2, mRNA.

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CloneID	OMu16623
Clone ID Related Accession (Same CDS sequence)	NM_001163691.1
Accession Version	NM_001163691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK147242.1, AC122454.4 and AI834839.1. Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of a coding exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAAGGCCCAG 
CGCCGGCCCT ACTACGCAGA CTATTCACAC ACGCGCCGCT CCATCCATTC GCTGTGCACC 
AGCCACTACC TGGACCTCTT CATCACCTTC ATCATCTGCC TCAATGTCAT CACCATGTCC 
ATGGAGCACT ACAACCAGCC CAAGTCTCTG GACGAGGCCC TTAAGTACTG CAACTACGTC 
TTTACCATCG TCTTCGTCTT TGAGGCCGCA CTGAAGCTGG TGGCCTTTGG ATTCCGGAGG 
TTCTTCAAGG ACAGGTGGAA CCAGCTGGAC TTGGCCATCG TCCTCCTGTC CATCATGGGC 
ATTGCGCTGG AGGAGATTGA GATGAATGCT GCCCTCCCCA TCAACCCCAC CATCATCCGC 
ATCATGCGCG TGCTTCGCAT CGCCCGTGTG CTGAAGCTAC TGAAGATGGC CACAGGCATG 
CGCGCCCTGC TGGACACTGT GGTTCAAGCT CTGCCTCAGG TAGGGAACCT TGGTCTTCTT 
TTCATGCTCC TGTTTTTTAT CTATGCTGCC CTGGGAGTGG AGCTGTTTGG GAGGCTAGAA 
TGCAGCGAGG ATAATCCCTG TGAGGGCCTG AGCAGACACG CCACCTTCAC CAACTTTGGC 
ATGGCCTTCC TCACGTTGTT CCGAGTGTCC ACTGGGGACA ACTGGAATGG GATCATGAAG 
GATACTCTCC GTGAATGTAC CCGTGAAGAC AAGCACTGCC TCAGCTACCT GCCCGCGCTC 
TCCCCCGTCT ACTTCGTCAC CTTCGTGCTG GTCGCCCAGT TCGTGCTGGT CAACGTGGTG 
GTGGCCGTGC TCATGAAGCA CCTGGAGGAG AGCAACAAGG AGGCCCGGGA GGATGCGGAG 
ATGGATGCCG AGATCGAGCT GGAGATCGCA CAGGGGTCCA CAGCCCAGCC CCCGTCCACA 
GCACAGGAAA GCCAAGGTAC CGAGCCAGAC ACCCCGAACC TCCTGGTCGT GCGCAAAGTA 
TCTGTGTCCA GGATGCTCTC GCTACCCAAT GACAGCTACA TGTTCCGGCC GGTGGCTCCT 
GCGGCTGCCC CGCATTCCCA CCCACTGCAG GAAGTGGAGA TGGAGACCTA CACAGGTCCC 
ATCAGCTGCG TCCTCCCCAG CCAGGGTCAG CGACCCCCTT TGTGCCCTCT CACCCCGGGA 
TACGCCCCGC TCTCTGAGCC TCTCACGGAT ACTCTGCAGA CAGGAGGCCA TGCACGCAGA 
GTCCCTGGAA GGGCAGATAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157163.1
CDS	333..6356
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQGTEPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGPISCVLPSQGQRPPLCPLTPGYAPLSEPLTDTLQTGGHARRVPGRADR

Target ORF information:

RefSeq Version	NM_001163691.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163691.1

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAAGGCCCAG 
CGCCGGCCCT ACTACGCAGA CTATTCACAC ACGCGCCGCT CCATCCATTC GCTGTGCACC 
AGCCACTACC TGGACCTCTT CATCACCTTC ATCATCTGCC TCAATGTCAT CACCATGTCC 
ATGGAGCACT ACAACCAGCC CAAGTCTCTG GACGAGGCCC TTAAGTACTG CAACTACGTC 
TTTACCATCG TCTTCGTCTT TGAGGCCGCA CTGAAGCTGG TGGCCTTTGG ATTCCGGAGG 
TTCTTCAAGG ACAGGTGGAA CCAGCTGGAC TTGGCCATCG TCCTCCTGTC CATCATGGGC 
ATTGCGCTGG AGGAGATTGA GATGAATGCT GCCCTCCCCA TCAACCCCAC CATCATCCGC 
ATCATGCGCG TGCTTCGCAT CGCCCGTGTG CTGAAGCTAC TGAAGATGGC CACAGGCATG 
CGCGCCCTGC TGGACACTGT GGTTCAAGCT CTGCCTCAGG TAGGGAACCT TGGTCTTCTT 
TTCATGCTCC TGTTTTTTAT CTATGCTGCC CTGGGAGTGG AGCTGTTTGG GAGGCTAGAA 
TGCAGCGAGG ATAATCCCTG TGAGGGCCTG AGCAGACACG CCACCTTCAC CAACTTTGGC 
ATGGCCTTCC TCACGTTGTT CCGAGTGTCC ACTGGGGACA ACTGGAATGG GATCATGAAG 
GATACTCTCC GTGAATGTAC CCGTGAAGAC AAGCACTGCC TCAGCTACCT GCCCGCGCTC 
TCCCCCGTCT ACTTCGTCAC CTTCGTGCTG GTCGCCCAGT TCGTGCTGGT CAACGTGGTG 
GTGGCCGTGC TCATGAAGCA CCTGGAGGAG AGCAACAAGG AGGCCCGGGA GGATGCGGAG 
ATGGATGCCG AGATCGAGCT GGAGATCGCA CAGGGGTCCA CAGCCCAGCC CCCGTCCACA 
GCACAGGAAA GCCAAGGTAC CGAGCCAGAC ACCCCGAACC TCCTGGTCGT GCGCAAAGTA 
TCTGTGTCCA GGATGCTCTC GCTACCCAAT GACAGCTACA TGTTCCGGCC GGTGGCTCCT 
GCGGCTGCCC CGCATTCCCA CCCACTGCAG GAAGTGGAGA TGGAGACCTA CACAGGTCCC 
ATCAGCTGCG TCCTCCCCAG CCAGGGTCAG CGACCCCCTT TGTGCCCTCT CACCCCGGGA 
TACGCCCCGC TCTCTGAGCC TCTCACGGAT ACTCTGCAGA CAGGAGGCCA TGCACGCAGA 
GTCCCTGGAA GGGCAGATAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1h ( XM_006524733.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524796.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X1, mRNA.

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CloneID	OMu53886
Clone ID Related Accession (Same CDS sequence)	XM_006524733.4 , XM_006524733.3
Accession Version	XM_006524733.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524733.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGTAAGGCG 
GTCCCCGAGG TGGAGGAGGC CCAGCGCCGG CCCTACTACG CAGACTATTC ACACACGCGC 
CGCTCCATCC ATTCGCTGTG CACCAGCCAC TACCTGGACC TCTTCATCAC CTTCATCATC 
TGCCTCAATG TCATCACCAT GTCCATGGAG CACTACAACC AGCCCAAGTC TCTGGACGAG 
GCCCTTAAGT ACTGCAACTA CGTCTTTACC ATCGTCTTCG TCTTTGAGGC CGCACTGAAG 
CTGGTGGCCT TTGGATTCCG GAGGTTCTTC AAGGACAGGT GGAACCAGCT GGACTTGGCC 
ATCGTCCTCC TGTCCATCAT GGGCATTGCG CTGGAGGAGA TTGAGATGAA TGCTGCCCTC 
CCCATCAACC CCACCATCAT CCGCATCATG CGCGTGCTTC GCATCGCCCG TGTGCTGAAG 
CTACTGAAGA TGGCCACAGG CATGCGCGCC CTGCTGGACA CTGTGGTTCA AGCTCTGCCT 
CAGGTAGGGA ACCTTGGTCT TCTTTTCATG CTCCTGTTTT TTATCTATGC TGCCCTGGGA 
GTGGAGCTGT TTGGGAGGCT AGAATGCAGC GAGGATAATC CCTGTGAGGG CCTGAGCAGA 
CACGCCACCT TCACCAACTT TGGCATGGCC TTCCTCACGT TGTTCCGAGT GTCCACTGGG 
GACAACTGGA ATGGGATCAT GAAGGATACT CTCCGTGAAT GTACCCGTGA AGACAAGCAC 
TGCCTCAGCT ACCTGCCCGC GCTCTCCCCC GTCTACTTCG TCACCTTCGT GCTGGTCGCC 
CAGTTCGTGC TGGTCAACGT GGTGGTGGCC GTGCTCATGA AGCACCTGGA GGAGAGCAAC 
AAGGAGGCCC GGGAGGATGC GGAGATGGAT GCCGAGATCG AGCTGGAGAT CGCACAGGGG 
TCCACAGCCC AGCCCCCGTC CACAGCACAG GAAAGCCAAG GTACCGAGCC AGACACCCCG 
AACCTCCTGG TCGTGCGCAA AGTATCTGTG TCCAGGATGC TCTCGCTACC CAATGACAGC 
TACATGTTCC GGCCGGTGGC TCCTGCGGCT GCCCCGCATT CCCACCCACT GCAGGAAGTG 
GAGATGGAGA CCTACACAGG CCCGGTCACC TCTGCTCACT CGCCATCCCT GGAGCCCCGC 
ACCTCCTTCC AGGTCCCATC AGCTGCGTCC TCCCCAGCCA GGGTCAGCGA CCCCCTTTGT 
GCCCTCTCAC CCCGGGATAC GCCCCGCTCT CTGAGCCTCT CACGGATACT CTGCAGACAG 
GAGGCCATGC ACGCAGAGTC CCTGGAAGGG CAGATAGATG ACGCTGGAGA AGACAGCATC 
CCAGACTACA CGGAGCCTGC TGAAAATATC TCCATGAGCC AGGCACCTCT GGGTACCCTG 
CGGTCTCCTC CATGCTCCCC ACGGCCTGCC AGTGTCCGTA CCCGCAAGCA CACTTTTGGG 
CAGCACTGCA TCTCCAGCCG CCCTCCCACC CTGGGAGGAG ATGATGCTGA AGCAGCAGAC 
CCGGCCGATG AGGAGGTCAG CCACATCACC AGCTCAGCCC ACCCTTGGCC AGCTACAGAG 
CCCCACAGCC CAGAGGCCTC CCCGACAGCC TCTCCTGCAA AAGGCACAGT GGGCAGTGGG 
CGGGACCCAC ACAGGTTCTG CAGTGTAGAT GCTCAGAGCT TCCTGGACAA ACCAGGTCGG 
CCAGATGCAC AGCGGTGGTC CTCGGTGGAA CTGGATAATG GGGATGGCCA CCTAGAGTCT 
GGGGAAGTGA GGGCCCGGGC CTCAGAGCTA GAACCAGCTC TTGGGGCACG GAGGAAGAAG 
AAGATGAGCC CTCCCTGCAT CTCCATTGAC CCTCCCACTG AAGATGAGGG CTCCTCCCGC 
CCCCCTGCAG CAGAAGGAGG CAACACTACT CTGAGGCGCC GAACCCCGTC CTGTGAGGCT 
GCCCTCCATA GGGACTGCCC AGAGTCTACA GAGGGCCCAG GCACTGGAGG GGACCCTGTA 
GCCAAGGGTG AGCGCTGGGG CCAGGCCTCT TGCCGGGCAG AGCATCTGAC TGTCCCCAAC 
TTTGCCTTTG AGCCTCTGGA CATGGGCGGA CCTGGTGGAG ACTGTTTCTT GGACAGTGAC 
CAAAGTGTGA CCCCAGAACC CAGAGTTTCC TCTTTGGGGG CTATAGTGCC TCTAATACTA 
GAAACTGAAC TTTCTATGCC CTCTGGTGAC CCCCCAGAGA AGGAGCAAGG ACTGTACCTC 
ACTGTGCCCC AGACCCCCTT GAAGAAACCA GGGTCTCCCC CAGCCACTCC TGCCCCAGAT 
GACAGTGGAG ATGAGCCTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524796.1
CDS	2884..9987
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSKAVPEVEEAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQGTEPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGPVTSAHSPSLEPRTSFQVPSAASSPARVSDPLCALSPRDTPRSLSLSRILCRQEAMHAESLEGQIDDAGEDSIPDYTEPAENISMSQAPLGTLRSPPCSPRPASVRTRKHTFGQHCISSRPPTLGGDDAEAADPADEEVSHITSSAHPWPATEPHSPEASPTASPAKGTVGSGRDPHRFCSVDAQSFLDKPGRPDAQRWSSVELDNGDGHLESGEVRARASELEPALGARRKKKMSPPCISIDPPTEDEGSSRPPAAEGGNTTLRRRTPSCEAALHRDCPESTEGPGTGGDPVAKGERWGQASCRAEHLTVPNFAFEPLDMGGPGGDCFLDSDQSVTPEPRVSSLGAIVPLILETELSMPSGDPPEKEQGLYLTVPQTPLKKPGSPPATPAPDDSGDEPV

Target ORF information:

RefSeq Version	XM_006524733.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524733.4

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGTAAGGCG 
GTCCCCGAGG TGGAGGAGGC CCAGCGCCGG CCCTACTACG CAGACTATTC ACACACGCGC 
CGCTCCATCC ATTCGCTGTG CACCAGCCAC TACCTGGACC TCTTCATCAC CTTCATCATC 
TGCCTCAATG TCATCACCAT GTCCATGGAG CACTACAACC AGCCCAAGTC TCTGGACGAG 
GCCCTTAAGT ACTGCAACTA CGTCTTTACC ATCGTCTTCG TCTTTGAGGC CGCACTGAAG 
CTGGTGGCCT TTGGATTCCG GAGGTTCTTC AAGGACAGGT GGAACCAGCT GGACTTGGCC 
ATCGTCCTCC TGTCCATCAT GGGCATTGCG CTGGAGGAGA TTGAGATGAA TGCTGCCCTC 
CCCATCAACC CCACCATCAT CCGCATCATG CGCGTGCTTC GCATCGCCCG TGTGCTGAAG 
CTACTGAAGA TGGCCACAGG CATGCGCGCC CTGCTGGACA CTGTGGTTCA AGCTCTGCCT 
CAGGTAGGGA ACCTTGGTCT TCTTTTCATG CTCCTGTTTT TTATCTATGC TGCCCTGGGA 
GTGGAGCTGT TTGGGAGGCT AGAATGCAGC GAGGATAATC CCTGTGAGGG CCTGAGCAGA 
CACGCCACCT TCACCAACTT TGGCATGGCC TTCCTCACGT TGTTCCGAGT GTCCACTGGG 
GACAACTGGA ATGGGATCAT GAAGGATACT CTCCGTGAAT GTACCCGTGA AGACAAGCAC 
TGCCTCAGCT ACCTGCCCGC GCTCTCCCCC GTCTACTTCG TCACCTTCGT GCTGGTCGCC 
CAGTTCGTGC TGGTCAACGT GGTGGTGGCC GTGCTCATGA AGCACCTGGA GGAGAGCAAC 
AAGGAGGCCC GGGAGGATGC GGAGATGGAT GCCGAGATCG AGCTGGAGAT CGCACAGGGG 
TCCACAGCCC AGCCCCCGTC CACAGCACAG GAAAGCCAAG GTACCGAGCC AGACACCCCG 
AACCTCCTGG TCGTGCGCAA AGTATCTGTG TCCAGGATGC TCTCGCTACC CAATGACAGC 
TACATGTTCC GGCCGGTGGC TCCTGCGGCT GCCCCGCATT CCCACCCACT GCAGGAAGTG 
GAGATGGAGA CCTACACAGG CCCGGTCACC TCTGCTCACT CGCCATCCCT GGAGCCCCGC 
ACCTCCTTCC AGGTCCCATC AGCTGCGTCC TCCCCAGCCA GGGTCAGCGA CCCCCTTTGT 
GCCCTCTCAC CCCGGGATAC GCCCCGCTCT CTGAGCCTCT CACGGATACT CTGCAGACAG 
GAGGCCATGC ACGCAGAGTC CCTGGAAGGG CAGATAGATG ACGCTGGAGA AGACAGCATC 
CCAGACTACA CGGAGCCTGC TGAAAATATC TCCATGAGCC AGGCACCTCT GGGTACCCTG 
CGGTCTCCTC CATGCTCCCC ACGGCCTGCC AGTGTCCGTA CCCGCAAGCA CACTTTTGGG 
CAGCACTGCA TCTCCAGCCG CCCTCCCACC CTGGGAGGAG ATGATGCTGA AGCAGCAGAC 
CCGGCCGATG AGGAGGTCAG CCACATCACC AGCTCAGCCC ACCCTTGGCC AGCTACAGAG 
CCCCACAGCC CAGAGGCCTC CCCGACAGCC TCTCCTGCAA AAGGCACAGT GGGCAGTGGG 
CGGGACCCAC ACAGGTTCTG CAGTGTAGAT GCTCAGAGCT TCCTGGACAA ACCAGGTCGG 
CCAGATGCAC AGCGGTGGTC CTCGGTGGAA CTGGATAATG GGGATGGCCA CCTAGAGTCT 
GGGGAAGTGA GGGCCCGGGC CTCAGAGCTA GAACCAGCTC TTGGGGCACG GAGGAAGAAG 
AAGATGAGCC CTCCCTGCAT CTCCATTGAC CCTCCCACTG AAGATGAGGG CTCCTCCCGC 
CCCCCTGCAG CAGAAGGAGG CAACACTACT CTGAGGCGCC GAACCCCGTC CTGTGAGGCT 
GCCCTCCATA GGGACTGCCC AGAGTCTACA GAGGGCCCAG GCACTGGAGG GGACCCTGTA 
GCCAAGGGTG AGCGCTGGGG CCAGGCCTCT TGCCGGGCAG AGCATCTGAC TGTCCCCAAC 
TTTGCCTTTG AGCCTCTGGA CATGGGCGGA CCTGGTGGAG ACTGTTTCTT GGACAGTGAC 
CAAAGTGTGA CCCCAGAACC CAGAGTTTCC TCTTTGGGGG CTATAGTGCC TCTAATACTA 
GAAACTGAAC TTTCTATGCC CTCTGGTGAC CCCCCAGAGA AGGAGCAAGG ACTGTACCTC 
ACTGTGCCCC AGACCCCCTT GAAGAAACCA GGGTCTCCCC CAGCCACTCC TGCCCCAGAT 
GACAGTGGAG ATGAGCCTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1h ( XM_006524733.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524796.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X1, mRNA.

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CloneID	OMu53886
Clone ID Related Accession (Same CDS sequence)	XM_006524733.4 , XM_006524733.3
Accession Version	XM_006524733.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGTAAGGCG 
GTCCCCGAGG TGGAGGAGGC CCAGCGCCGG CCCTACTACG CAGACTATTC ACACACGCGC 
CGCTCCATCC ATTCGCTGTG CACCAGCCAC TACCTGGACC TCTTCATCAC CTTCATCATC 
TGCCTCAATG TCATCACCAT GTCCATGGAG CACTACAACC AGCCCAAGTC TCTGGACGAG 
GCCCTTAAGT ACTGCAACTA CGTCTTTACC ATCGTCTTCG TCTTTGAGGC CGCACTGAAG 
CTGGTGGCCT TTGGATTCCG GAGGTTCTTC AAGGACAGGT GGAACCAGCT GGACTTGGCC 
ATCGTCCTCC TGTCCATCAT GGGCATTGCG CTGGAGGAGA TTGAGATGAA TGCTGCCCTC 
CCCATCAACC CCACCATCAT CCGCATCATG CGCGTGCTTC GCATCGCCCG TGTGCTGAAG 
CTACTGAAGA TGGCCACAGG CATGCGCGCC CTGCTGGACA CTGTGGTTCA AGCTCTGCCT 
CAGGTAGGGA ACCTTGGTCT TCTTTTCATG CTCCTGTTTT TTATCTATGC TGCCCTGGGA 
GTGGAGCTGT TTGGGAGGCT AGAATGCAGC GAGGATAATC CCTGTGAGGG CCTGAGCAGA 
CACGCCACCT TCACCAACTT TGGCATGGCC TTCCTCACGT TGTTCCGAGT GTCCACTGGG 
GACAACTGGA ATGGGATCAT GAAGGATACT CTCCGTGAAT GTACCCGTGA AGACAAGCAC 
TGCCTCAGCT ACCTGCCCGC GCTCTCCCCC GTCTACTTCG TCACCTTCGT GCTGGTCGCC 
CAGTTCGTGC TGGTCAACGT GGTGGTGGCC GTGCTCATGA AGCACCTGGA GGAGAGCAAC 
AAGGAGGCCC GGGAGGATGC GGAGATGGAT GCCGAGATCG AGCTGGAGAT CGCACAGGGG 
TCCACAGCCC AGCCCCCGTC CACAGCACAG GAAAGCCAAG GTACCGAGCC AGACACCCCG 
AACCTCCTGG TCGTGCGCAA AGTATCTGTG TCCAGGATGC TCTCGCTACC CAATGACAGC 
TACATGTTCC GGCCGGTGGC TCCTGCGGCT GCCCCGCATT CCCACCCACT GCAGGAAGTG 
GAGATGGAGA CCTACACAGG CCCGGTCACC TCTGCTCACT CGCCATCCCT GGAGCCCCGC 
ACCTCCTTCC AGGTCCCATC AGCTGCGTCC TCCCCAGCCA GGGTCAGCGA CCCCCTTTGT 
GCCCTCTCAC CCCGGGATAC GCCCCGCTCT CTGAGCCTCT CACGGATACT CTGCAGACAG 
GAGGCCATGC ACGCAGAGTC CCTGGAAGGG CAGATAGATG ACGCTGGAGA AGACAGCATC 
CCAGACTACA CGGAGCCTGC TGAAAATATC TCCATGAGCC AGGCACCTCT GGGTACCCTG 
CGGTCTCCTC CATGCTCCCC ACGGCCTGCC AGTGTCCGTA CCCGCAAGCA CACTTTTGGG 
CAGCACTGCA TCTCCAGCCG CCCTCCCACC CTGGGAGGAG ATGATGCTGA AGCAGCAGAC 
CCGGCCGATG AGGAGGTCAG CCACATCACC AGCTCAGCCC ACCCTTGGCC AGCTACAGAG 
CCCCACAGCC CAGAGGCCTC CCCGACAGCC TCTCCTGCAA AAGGCACAGT GGGCAGTGGG 
CGGGACCCAC ACAGGTTCTG CAGTGTAGAT GCTCAGAGCT TCCTGGACAA ACCAGGTCGG 
CCAGATGCAC AGCGGTGGTC CTCGGTGGAA CTGGATAATG GGGATGGCCA CCTAGAGTCT 
GGGGAAGTGA GGGCCCGGGC CTCAGAGCTA GAACCAGCTC TTGGGGCACG GAGGAAGAAG 
AAGATGAGCC CTCCCTGCAT CTCCATTGAC CCTCCCACTG AAGATGAGGG CTCCTCCCGC 
CCCCCTGCAG CAGAAGGAGG CAACACTACT CTGAGGCGCC GAACCCCGTC CTGTGAGGCT 
GCCCTCCATA GGGACTGCCC AGAGTCTACA GAGGGCCCAG GCACTGGAGG GGACCCTGTA 
GCCAAGGGTG AGCGCTGGGG CCAGGCCTCT TGCCGGGCAG AGCATCTGAC TGTCCCCAAC 
TTTGCCTTTG AGCCTCTGGA CATGGGCGGA CCTGGTGGAG ACTGTTTCTT GGACAGTGAC 
CAAAGTGTGA CCCCAGAACC CAGAGTTTCC TCTTTGGGGG CTATAGTGCC TCTAATACTA 
GAAACTGAAC TTTCTATGCC CTCTGGTGAC CCCCCAGAGA AGGAGCAAGG ACTGTACCTC 
ACTGTGCCCC AGACCCCCTT GAAGAAACCA GGGTCTCCCC CAGCCACTCC TGCCCCAGAT 
GACAGTGGAG ATGAGCCTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524796.1
CDS	326..7429
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSKAVPEVEEAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQGTEPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGPVTSAHSPSLEPRTSFQVPSAASSPARVSDPLCALSPRDTPRSLSLSRILCRQEAMHAESLEGQIDDAGEDSIPDYTEPAENISMSQAPLGTLRSPPCSPRPASVRTRKHTFGQHCISSRPPTLGGDDAEAADPADEEVSHITSSAHPWPATEPHSPEASPTASPAKGTVGSGRDPHRFCSVDAQSFLDKPGRPDAQRWSSVELDNGDGHLESGEVRARASELEPALGARRKKKMSPPCISIDPPTEDEGSSRPPAAEGGNTTLRRRTPSCEAALHRDCPESTEGPGTGGDPVAKGERWGQASCRAEHLTVPNFAFEPLDMGGPGGDCFLDSDQSVTPEPRVSSLGAIVPLILETELSMPSGDPPEKEQGLYLTVPQTPLKKPGSPPATPAPDDSGDEPV

Target ORF information:

RefSeq Version	XM_006524733.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524733.3

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGTAAGGCG 
GTCCCCGAGG TGGAGGAGGC CCAGCGCCGG CCCTACTACG CAGACTATTC ACACACGCGC 
CGCTCCATCC ATTCGCTGTG CACCAGCCAC TACCTGGACC TCTTCATCAC CTTCATCATC 
TGCCTCAATG TCATCACCAT GTCCATGGAG CACTACAACC AGCCCAAGTC TCTGGACGAG 
GCCCTTAAGT ACTGCAACTA CGTCTTTACC ATCGTCTTCG TCTTTGAGGC CGCACTGAAG 
CTGGTGGCCT TTGGATTCCG GAGGTTCTTC AAGGACAGGT GGAACCAGCT GGACTTGGCC 
ATCGTCCTCC TGTCCATCAT GGGCATTGCG CTGGAGGAGA TTGAGATGAA TGCTGCCCTC 
CCCATCAACC CCACCATCAT CCGCATCATG CGCGTGCTTC GCATCGCCCG TGTGCTGAAG 
CTACTGAAGA TGGCCACAGG CATGCGCGCC CTGCTGGACA CTGTGGTTCA AGCTCTGCCT 
CAGGTAGGGA ACCTTGGTCT TCTTTTCATG CTCCTGTTTT TTATCTATGC TGCCCTGGGA 
GTGGAGCTGT TTGGGAGGCT AGAATGCAGC GAGGATAATC CCTGTGAGGG CCTGAGCAGA 
CACGCCACCT TCACCAACTT TGGCATGGCC TTCCTCACGT TGTTCCGAGT GTCCACTGGG 
GACAACTGGA ATGGGATCAT GAAGGATACT CTCCGTGAAT GTACCCGTGA AGACAAGCAC 
TGCCTCAGCT ACCTGCCCGC GCTCTCCCCC GTCTACTTCG TCACCTTCGT GCTGGTCGCC 
CAGTTCGTGC TGGTCAACGT GGTGGTGGCC GTGCTCATGA AGCACCTGGA GGAGAGCAAC 
AAGGAGGCCC GGGAGGATGC GGAGATGGAT GCCGAGATCG AGCTGGAGAT CGCACAGGGG 
TCCACAGCCC AGCCCCCGTC CACAGCACAG GAAAGCCAAG GTACCGAGCC AGACACCCCG 
AACCTCCTGG TCGTGCGCAA AGTATCTGTG TCCAGGATGC TCTCGCTACC CAATGACAGC 
TACATGTTCC GGCCGGTGGC TCCTGCGGCT GCCCCGCATT CCCACCCACT GCAGGAAGTG 
GAGATGGAGA CCTACACAGG CCCGGTCACC TCTGCTCACT CGCCATCCCT GGAGCCCCGC 
ACCTCCTTCC AGGTCCCATC AGCTGCGTCC TCCCCAGCCA GGGTCAGCGA CCCCCTTTGT 
GCCCTCTCAC CCCGGGATAC GCCCCGCTCT CTGAGCCTCT CACGGATACT CTGCAGACAG 
GAGGCCATGC ACGCAGAGTC CCTGGAAGGG CAGATAGATG ACGCTGGAGA AGACAGCATC 
CCAGACTACA CGGAGCCTGC TGAAAATATC TCCATGAGCC AGGCACCTCT GGGTACCCTG 
CGGTCTCCTC CATGCTCCCC ACGGCCTGCC AGTGTCCGTA CCCGCAAGCA CACTTTTGGG 
CAGCACTGCA TCTCCAGCCG CCCTCCCACC CTGGGAGGAG ATGATGCTGA AGCAGCAGAC 
CCGGCCGATG AGGAGGTCAG CCACATCACC AGCTCAGCCC ACCCTTGGCC AGCTACAGAG 
CCCCACAGCC CAGAGGCCTC CCCGACAGCC TCTCCTGCAA AAGGCACAGT GGGCAGTGGG 
CGGGACCCAC ACAGGTTCTG CAGTGTAGAT GCTCAGAGCT TCCTGGACAA ACCAGGTCGG 
CCAGATGCAC AGCGGTGGTC CTCGGTGGAA CTGGATAATG GGGATGGCCA CCTAGAGTCT 
GGGGAAGTGA GGGCCCGGGC CTCAGAGCTA GAACCAGCTC TTGGGGCACG GAGGAAGAAG 
AAGATGAGCC CTCCCTGCAT CTCCATTGAC CCTCCCACTG AAGATGAGGG CTCCTCCCGC 
CCCCCTGCAG CAGAAGGAGG CAACACTACT CTGAGGCGCC GAACCCCGTC CTGTGAGGCT 
GCCCTCCATA GGGACTGCCC AGAGTCTACA GAGGGCCCAG GCACTGGAGG GGACCCTGTA 
GCCAAGGGTG AGCGCTGGGG CCAGGCCTCT TGCCGGGCAG AGCATCTGAC TGTCCCCAAC 
TTTGCCTTTG AGCCTCTGGA CATGGGCGGA CCTGGTGGAG ACTGTTTCTT GGACAGTGAC 
CAAAGTGTGA CCCCAGAACC CAGAGTTTCC TCTTTGGGGG CTATAGTGCC TCTAATACTA 
GAAACTGAAC TTTCTATGCC CTCTGGTGAC CCCCCAGAGA AGGAGCAAGG ACTGTACCTC 
ACTGTGCCCC AGACCCCCTT GAAGAAACCA GGGTCTCCCC CAGCCACTCC TGCCCCAGAT 
GACAGTGGAG ATGAGCCTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1h ( XM_006524734.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524797.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X2, mRNA.

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CloneID	OMu53887
Clone ID Related Accession (Same CDS sequence)	XM_006524734.3 , XM_006524734.4
Accession Version	XM_006524734.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGCACTTTC 
CCCAACCCAG AGGCCCAGCG CCGGCCCTAC TACGCAGACT ATTCACACAC GCGCCGCTCC 
ATCCATTCGC TGTGCACCAG CCACTACCTG GACCTCTTCA TCACCTTCAT CATCTGCCTC 
AATGTCATCA CCATGTCCAT GGAGCACTAC AACCAGCCCA AGTCTCTGGA CGAGGCCCTT 
AAGTACTGCA ACTACGTCTT TACCATCGTC TTCGTCTTTG AGGCCGCACT GAAGCTGGTG 
GCCTTTGGAT TCCGGAGGTT CTTCAAGGAC AGGTGGAACC AGCTGGACTT GGCCATCGTC 
CTCCTGTCCA TCATGGGCAT TGCGCTGGAG GAGATTGAGA TGAATGCTGC CCTCCCCATC 
AACCCCACCA TCATCCGCAT CATGCGCGTG CTTCGCATCG CCCGTGTGCT GAAGCTACTG 
AAGATGGCCA CAGGCATGCG CGCCCTGCTG GACACTGTGG TTCAAGCTCT GCCTCAGGTA 
GGGAACCTTG GTCTTCTTTT CATGCTCCTG TTTTTTATCT ATGCTGCCCT GGGAGTGGAG 
CTGTTTGGGA GGCTAGAATG CAGCGAGGAT AATCCCTGTG AGGGCCTGAG CAGACACGCC 
ACCTTCACCA ACTTTGGCAT GGCCTTCCTC ACGTTGTTCC GAGTGTCCAC TGGGGACAAC 
TGGAATGGGA TCATGAAGGA TACTCTCCGT GAATGTACCC GTGAAGACAA GCACTGCCTC 
AGCTACCTGC CCGCGCTCTC CCCCGTCTAC TTCGTCACCT TCGTGCTGGT CGCCCAGTTC 
GTGCTGGTCA ACGTGGTGGT GGCCGTGCTC ATGAAGCACC TGGAGGAGAG CAACAAGGAG 
GCCCGGGAGG ATGCGGAGAT GGATGCCGAG ATCGAGCTGG AGATCGCACA GGGGTCCACA 
GCCCAGCCCC CGTCCACAGC ACAGGAAAGC CAAGGTACCG AGCCAGACAC CCCGAACCTC 
CTGGTCGTGC GCAAAGTATC TGTGTCCAGG ATGCTCTCGC TACCCAATGA CAGCTACATG 
TTCCGGCCGG TGGCTCCTGC GGCTGCCCCG CATTCCCACC CACTGCAGGA AGTGGAGATG 
GAGACCTACA CAGGCCCGGT CACCTCTGCT CACTCGCCAT CCCTGGAGCC CCGCACCTCC 
TTCCAGGTCC CATCAGCTGC GTCCTCCCCA GCCAGGGTCA GCGACCCCCT TTGTGCCCTC 
TCACCCCGGG ATACGCCCCG CTCTCTGAGC CTCTCACGGA TACTCTGCAG ACAGGAGGCC 
ATGCACGCAG AGTCCCTGGA AGGGCAGATA GATGACGCTG GAGAAGACAG CATCCCAGAC 
TACACGGAGC CTGCTGAAAA TATCTCCATG AGCCAGGCAC CTCTGGGTAC CCTGCGGTCT 
CCTCCATGCT CCCCACGGCC TGCCAGTGTC CGTACCCGCA AGCACACTTT TGGGCAGCAC 
TGCATCTCCA GCCGCCCTCC CACCCTGGGA GGAGATGATG CTGAAGCAGC AGACCCGGCC 
GATGAGGAGG TCAGCCACAT CACCAGCTCA GCCCACCCTT GGCCAGCTAC AGAGCCCCAC 
AGCCCAGAGG CCTCCCCGAC AGCCTCTCCT GCAAAAGGCA CAGTGGGCAG TGGGCGGGAC 
CCACACAGGT TCTGCAGTGT AGATGCTCAG AGCTTCCTGG ACAAACCAGG TCGGCCAGAT 
GCACAGCGGT GGTCCTCGGT GGAACTGGAT AATGGGGATG GCCACCTAGA GTCTGGGGAA 
GTGAGGGCCC GGGCCTCAGA GCTAGAACCA GCTCTTGGGG CACGGAGGAA GAAGAAGATG 
AGCCCTCCCT GCATCTCCAT TGACCCTCCC ACTGAAGATG AGGGCTCCTC CCGCCCCCCT 
GCAGCAGAAG GAGGCAACAC TACTCTGAGG CGCCGAACCC CGTCCTGTGA GGCTGCCCTC 
CATAGGGACT GCCCAGAGTC TACAGAGGGC CCAGGCACTG GAGGGGACCC TGTAGCCAAG 
GGTGAGCGCT GGGGCCAGGC CTCTTGCCGG GCAGAGCATC TGACTGTCCC CAACTTTGCC 
TTTGAGCCTC TGGACATGGG CGGACCTGGT GGAGACTGTT TCTTGGACAG TGACCAAAGT 
GTGACCCCAG AACCCAGAGT TTCCTCTTTG GGGGCTATAG TGCCTCTAAT ACTAGAAACT 
GAACTTTCTA TGCCCTCTGG TGACCCCCCA GAGAAGGAGC AAGGACTGTA CCTCACTGTG 
CCCCAGACCC CCTTGAAGAA ACCAGGGTCT CCCCCAGCCA CTCCTGCCCC AGATGACAGT 
GGAGATGAGC CTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524797.1
CDS	326..7423
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSTFPNPEAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQGTEPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGPVTSAHSPSLEPRTSFQVPSAASSPARVSDPLCALSPRDTPRSLSLSRILCRQEAMHAESLEGQIDDAGEDSIPDYTEPAENISMSQAPLGTLRSPPCSPRPASVRTRKHTFGQHCISSRPPTLGGDDAEAADPADEEVSHITSSAHPWPATEPHSPEASPTASPAKGTVGSGRDPHRFCSVDAQSFLDKPGRPDAQRWSSVELDNGDGHLESGEVRARASELEPALGARRKKKMSPPCISIDPPTEDEGSSRPPAAEGGNTTLRRRTPSCEAALHRDCPESTEGPGTGGDPVAKGERWGQASCRAEHLTVPNFAFEPLDMGGPGGDCFLDSDQSVTPEPRVSSLGAIVPLILETELSMPSGDPPEKEQGLYLTVPQTPLKKPGSPPATPAPDDSGDEPV

Target ORF information:

RefSeq Version	XM_006524734.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524734.3

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGCACTTTC 
CCCAACCCAG AGGCCCAGCG CCGGCCCTAC TACGCAGACT ATTCACACAC GCGCCGCTCC 
ATCCATTCGC TGTGCACCAG CCACTACCTG GACCTCTTCA TCACCTTCAT CATCTGCCTC 
AATGTCATCA CCATGTCCAT GGAGCACTAC AACCAGCCCA AGTCTCTGGA CGAGGCCCTT 
AAGTACTGCA ACTACGTCTT TACCATCGTC TTCGTCTTTG AGGCCGCACT GAAGCTGGTG 
GCCTTTGGAT TCCGGAGGTT CTTCAAGGAC AGGTGGAACC AGCTGGACTT GGCCATCGTC 
CTCCTGTCCA TCATGGGCAT TGCGCTGGAG GAGATTGAGA TGAATGCTGC CCTCCCCATC 
AACCCCACCA TCATCCGCAT CATGCGCGTG CTTCGCATCG CCCGTGTGCT GAAGCTACTG 
AAGATGGCCA CAGGCATGCG CGCCCTGCTG GACACTGTGG TTCAAGCTCT GCCTCAGGTA 
GGGAACCTTG GTCTTCTTTT CATGCTCCTG TTTTTTATCT ATGCTGCCCT GGGAGTGGAG 
CTGTTTGGGA GGCTAGAATG CAGCGAGGAT AATCCCTGTG AGGGCCTGAG CAGACACGCC 
ACCTTCACCA ACTTTGGCAT GGCCTTCCTC ACGTTGTTCC GAGTGTCCAC TGGGGACAAC 
TGGAATGGGA TCATGAAGGA TACTCTCCGT GAATGTACCC GTGAAGACAA GCACTGCCTC 
AGCTACCTGC CCGCGCTCTC CCCCGTCTAC TTCGTCACCT TCGTGCTGGT CGCCCAGTTC 
GTGCTGGTCA ACGTGGTGGT GGCCGTGCTC ATGAAGCACC TGGAGGAGAG CAACAAGGAG 
GCCCGGGAGG ATGCGGAGAT GGATGCCGAG ATCGAGCTGG AGATCGCACA GGGGTCCACA 
GCCCAGCCCC CGTCCACAGC ACAGGAAAGC CAAGGTACCG AGCCAGACAC CCCGAACCTC 
CTGGTCGTGC GCAAAGTATC TGTGTCCAGG ATGCTCTCGC TACCCAATGA CAGCTACATG 
TTCCGGCCGG TGGCTCCTGC GGCTGCCCCG CATTCCCACC CACTGCAGGA AGTGGAGATG 
GAGACCTACA CAGGCCCGGT CACCTCTGCT CACTCGCCAT CCCTGGAGCC CCGCACCTCC 
TTCCAGGTCC CATCAGCTGC GTCCTCCCCA GCCAGGGTCA GCGACCCCCT TTGTGCCCTC 
TCACCCCGGG ATACGCCCCG CTCTCTGAGC CTCTCACGGA TACTCTGCAG ACAGGAGGCC 
ATGCACGCAG AGTCCCTGGA AGGGCAGATA GATGACGCTG GAGAAGACAG CATCCCAGAC 
TACACGGAGC CTGCTGAAAA TATCTCCATG AGCCAGGCAC CTCTGGGTAC CCTGCGGTCT 
CCTCCATGCT CCCCACGGCC TGCCAGTGTC CGTACCCGCA AGCACACTTT TGGGCAGCAC 
TGCATCTCCA GCCGCCCTCC CACCCTGGGA GGAGATGATG CTGAAGCAGC AGACCCGGCC 
GATGAGGAGG TCAGCCACAT CACCAGCTCA GCCCACCCTT GGCCAGCTAC AGAGCCCCAC 
AGCCCAGAGG CCTCCCCGAC AGCCTCTCCT GCAAAAGGCA CAGTGGGCAG TGGGCGGGAC 
CCACACAGGT TCTGCAGTGT AGATGCTCAG AGCTTCCTGG ACAAACCAGG TCGGCCAGAT 
GCACAGCGGT GGTCCTCGGT GGAACTGGAT AATGGGGATG GCCACCTAGA GTCTGGGGAA 
GTGAGGGCCC GGGCCTCAGA GCTAGAACCA GCTCTTGGGG CACGGAGGAA GAAGAAGATG 
AGCCCTCCCT GCATCTCCAT TGACCCTCCC ACTGAAGATG AGGGCTCCTC CCGCCCCCCT 
GCAGCAGAAG GAGGCAACAC TACTCTGAGG CGCCGAACCC CGTCCTGTGA GGCTGCCCTC 
CATAGGGACT GCCCAGAGTC TACAGAGGGC CCAGGCACTG GAGGGGACCC TGTAGCCAAG 
GGTGAGCGCT GGGGCCAGGC CTCTTGCCGG GCAGAGCATC TGACTGTCCC CAACTTTGCC 
TTTGAGCCTC TGGACATGGG CGGACCTGGT GGAGACTGTT TCTTGGACAG TGACCAAAGT 
GTGACCCCAG AACCCAGAGT TTCCTCTTTG GGGGCTATAG TGCCTCTAAT ACTAGAAACT 
GAACTTTCTA TGCCCTCTGG TGACCCCCCA GAGAAGGAGC AAGGACTGTA CCTCACTGTG 
CCCCAGACCC CCTTGAAGAA ACCAGGGTCT CCCCCAGCCA CTCCTGCCCC AGATGACAGT 
GGAGATGAGC CTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1h ( XM_006524734.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524797.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X2, mRNA.

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CloneID	OMu53887
Clone ID Related Accession (Same CDS sequence)	XM_006524734.3 , XM_006524734.4
Accession Version	XM_006524734.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524734.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGCACTTTC 
CCCAACCCAG AGGCCCAGCG CCGGCCCTAC TACGCAGACT ATTCACACAC GCGCCGCTCC 
ATCCATTCGC TGTGCACCAG CCACTACCTG GACCTCTTCA TCACCTTCAT CATCTGCCTC 
AATGTCATCA CCATGTCCAT GGAGCACTAC AACCAGCCCA AGTCTCTGGA CGAGGCCCTT 
AAGTACTGCA ACTACGTCTT TACCATCGTC TTCGTCTTTG AGGCCGCACT GAAGCTGGTG 
GCCTTTGGAT TCCGGAGGTT CTTCAAGGAC AGGTGGAACC AGCTGGACTT GGCCATCGTC 
CTCCTGTCCA TCATGGGCAT TGCGCTGGAG GAGATTGAGA TGAATGCTGC CCTCCCCATC 
AACCCCACCA TCATCCGCAT CATGCGCGTG CTTCGCATCG CCCGTGTGCT GAAGCTACTG 
AAGATGGCCA CAGGCATGCG CGCCCTGCTG GACACTGTGG TTCAAGCTCT GCCTCAGGTA 
GGGAACCTTG GTCTTCTTTT CATGCTCCTG TTTTTTATCT ATGCTGCCCT GGGAGTGGAG 
CTGTTTGGGA GGCTAGAATG CAGCGAGGAT AATCCCTGTG AGGGCCTGAG CAGACACGCC 
ACCTTCACCA ACTTTGGCAT GGCCTTCCTC ACGTTGTTCC GAGTGTCCAC TGGGGACAAC 
TGGAATGGGA TCATGAAGGA TACTCTCCGT GAATGTACCC GTGAAGACAA GCACTGCCTC 
AGCTACCTGC CCGCGCTCTC CCCCGTCTAC TTCGTCACCT TCGTGCTGGT CGCCCAGTTC 
GTGCTGGTCA ACGTGGTGGT GGCCGTGCTC ATGAAGCACC TGGAGGAGAG CAACAAGGAG 
GCCCGGGAGG ATGCGGAGAT GGATGCCGAG ATCGAGCTGG AGATCGCACA GGGGTCCACA 
GCCCAGCCCC CGTCCACAGC ACAGGAAAGC CAAGGTACCG AGCCAGACAC CCCGAACCTC 
CTGGTCGTGC GCAAAGTATC TGTGTCCAGG ATGCTCTCGC TACCCAATGA CAGCTACATG 
TTCCGGCCGG TGGCTCCTGC GGCTGCCCCG CATTCCCACC CACTGCAGGA AGTGGAGATG 
GAGACCTACA CAGGCCCGGT CACCTCTGCT CACTCGCCAT CCCTGGAGCC CCGCACCTCC 
TTCCAGGTCC CATCAGCTGC GTCCTCCCCA GCCAGGGTCA GCGACCCCCT TTGTGCCCTC 
TCACCCCGGG ATACGCCCCG CTCTCTGAGC CTCTCACGGA TACTCTGCAG ACAGGAGGCC 
ATGCACGCAG AGTCCCTGGA AGGGCAGATA GATGACGCTG GAGAAGACAG CATCCCAGAC 
TACACGGAGC CTGCTGAAAA TATCTCCATG AGCCAGGCAC CTCTGGGTAC CCTGCGGTCT 
CCTCCATGCT CCCCACGGCC TGCCAGTGTC CGTACCCGCA AGCACACTTT TGGGCAGCAC 
TGCATCTCCA GCCGCCCTCC CACCCTGGGA GGAGATGATG CTGAAGCAGC AGACCCGGCC 
GATGAGGAGG TCAGCCACAT CACCAGCTCA GCCCACCCTT GGCCAGCTAC AGAGCCCCAC 
AGCCCAGAGG CCTCCCCGAC AGCCTCTCCT GCAAAAGGCA CAGTGGGCAG TGGGCGGGAC 
CCACACAGGT TCTGCAGTGT AGATGCTCAG AGCTTCCTGG ACAAACCAGG TCGGCCAGAT 
GCACAGCGGT GGTCCTCGGT GGAACTGGAT AATGGGGATG GCCACCTAGA GTCTGGGGAA 
GTGAGGGCCC GGGCCTCAGA GCTAGAACCA GCTCTTGGGG CACGGAGGAA GAAGAAGATG 
AGCCCTCCCT GCATCTCCAT TGACCCTCCC ACTGAAGATG AGGGCTCCTC CCGCCCCCCT 
GCAGCAGAAG GAGGCAACAC TACTCTGAGG CGCCGAACCC CGTCCTGTGA GGCTGCCCTC 
CATAGGGACT GCCCAGAGTC TACAGAGGGC CCAGGCACTG GAGGGGACCC TGTAGCCAAG 
GGTGAGCGCT GGGGCCAGGC CTCTTGCCGG GCAGAGCATC TGACTGTCCC CAACTTTGCC 
TTTGAGCCTC TGGACATGGG CGGACCTGGT GGAGACTGTT TCTTGGACAG TGACCAAAGT 
GTGACCCCAG AACCCAGAGT TTCCTCTTTG GGGGCTATAG TGCCTCTAAT ACTAGAAACT 
GAACTTTCTA TGCCCTCTGG TGACCCCCCA GAGAAGGAGC AAGGACTGTA CCTCACTGTG 
CCCCAGACCC CCTTGAAGAA ACCAGGGTCT CCCCCAGCCA CTCCTGCCCC AGATGACAGT 
GGAGATGAGC CTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524797.1
CDS	2884..9981
Translation	MTEGTLAADEVRVPLGASPSAPAAPVRASPASPGVPGREEQRGSGSSVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASLKLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRLWASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDVQATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPHLDMAHTLLDSRRSSSGSVDPQLGDQKSLASLRSSPCAPWGPNSAGSSRRSSWNSLGRAPSLKRRSQCGERESLLSGEGKGSTDDEAEDSRPNSGTHPGASPGPRATPLRRAESLGHRSTMDLCPPRPATLLPTKFRDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCCFRLHKVLEPYAPQWCSSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSTFPNPEAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQGTEPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGPVTSAHSPSLEPRTSFQVPSAASSPARVSDPLCALSPRDTPRSLSLSRILCRQEAMHAESLEGQIDDAGEDSIPDYTEPAENISMSQAPLGTLRSPPCSPRPASVRTRKHTFGQHCISSRPPTLGGDDAEAADPADEEVSHITSSAHPWPATEPHSPEASPTASPAKGTVGSGRDPHRFCSVDAQSFLDKPGRPDAQRWSSVELDNGDGHLESGEVRARASELEPALGARRKKKMSPPCISIDPPTEDEGSSRPPAAEGGNTTLRRRTPSCEAALHRDCPESTEGPGTGGDPVAKGERWGQASCRAEHLTVPNFAFEPLDMGGPGGDCFLDSDQSVTPEPRVSSLGAIVPLILETELSMPSGDPPEKEQGLYLTVPQTPLKKPGSPPATPAPDDSGDEPV

Target ORF information:

RefSeq Version	XM_006524734.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524734.4

ORF Insert Sequence:

ATGACCGAGG GCACGCTGGC AGCGGACGAG GTCCGGGTGC CCCTGGGCGC TTCGCCTTCG 
GCTCCTGCAG CGCCGGTGAG GGCTTCCCCA GCGAGCCCTG GGGTGCCGGG GCGCGAGGAG 
CAGCGAGGAT CCGGGTCGAG CGTGTTGGCT CCCGAGAGCC CAGGGACCGA GTGTGGTGCG 
GACCTGGGCG CCGACGAGGA ACAGCCCGTC CCATACCCCG CTCTGGCTGC CACAGTCTTC 
TTCTGCCTCG GGCAAACCAC GCGGCCCCGC AGCTGGTGCC TCCGACTGGT TTGTAACCCG 
TGGTTCGAGC ACATTAGCAT GCTGGTCATC ATGCTGAACT GCGTGACACT GGGCATGTTC 
CGGCCCTGTG AGGATGTTGA GTGCCGTTCA GAACGTTGCA GCATCTTGGA GGCCTTTGAT 
GACTTCATCT TTGCCTTCTT TGCTGTGGAG ATGGTTATCA AGATGGTGGC TTTGGGACTG 
TTCGGCCAGA AATGCTACCT GGGTGACACC TGGAACAGGC TGGACTTCTT CATTGTCATG 
GCTGGCATGA TGGAATACTC TCTGGACGGA CACAACGTGA GCCTCTCTGC CATCCGAACA 
GTGCGTGTGC TGCGGCCCCT CCGCGCCATC AACCGGGTCC CCAGCATGCG AATCCTGGTC 
ACACTGCTGC TGGACACACT GCCCATGCTT GGGAACGTGC TTCTTCTCTG CTTCTTCGTC 
TTCTTCATCT TCGGCATCGT TGGGGTCCAG CTCTGGGCTG GCCTGCTGCG GAACCGATGC 
TTCCTGGACA GCGCCTTCGT CAGGAACAAC AACCTGACCT TCTTGCGGCC ATACTACCAG 
ACAGAGGAGG GCGAGGAGAA CCCATTCATC TGCTCCTCAC GCCGCGACAA CGGCATGCAG 
AAATGCTCAC ACATCCCCAG CCGCCGTGAG CTTCGCGTGC AGTGCACGCT CGGCTGGGAG 
GCCTACGGGC AGCCACAGGC TGAGGATGGG GGTGCCGGCC GAAATGCCTG CATCAACTGG 
AACCAGTACT ACAACGTGTG CCGCTCGGGG GAATTCAACC CTCACAATGG TGCCATCAAC 
TTTGACAACA TTGGCTATGC TTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTGGACATCA TGTACTACGT CATGGATGCC CACTCGTTCT ACAACTTCAT CTACTTCATC 
CTCCTCATCA TTGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATAGCCACA 
CAGTTCTCAG AGACAAAGCA GAGGGAAAAC CAGCTGATGC GTGAACAGCG GGCCCGCTAT 
CTGTCCAACG ACAGCACTCT GGCCAGCTTC TCGGAGCCTG GCAGCTGCTA CGAGGAGCTC 
CTCAAGTATG TGGGCCACAT CTTCCGAAAG GTTAAACGCC GGAGCCTGCG CCTTTACGCC 
CGCTGGCAGA GCCGTTGGCG TAAGAAGGTA GATCCCAGCA GCACTCTGCA TGGCCAAGGC 
CCCAGGCGGC GGCCACGGCG CGCAGGCAGG CGCACAGCTT CTGTGCACCA TCTGGTCTAC 
CACCACCACC ACCACCATCA CCACCATTAC CACTTCAGCC ACGGTGGCCC ACGCAGGCCT 
AGCCCAGAGC CAGGTGCTGG TGACACCAGG TTGGTCAGGG CCTGTGTGCC ACCCTCGCCA 
CCATCCCCAG GCCACGGGCC ACCAGACTCT GAGTCTGTGC ACAGTATCTA CCATGCTGAC 
TGCCACGTGG AGGGGCCACA GGAAAGAGCC CGTGTGGCGC ACACCATAGC CACTGCTGCC 
AGCCTCAAGC TGGCCTCAGG CTTGGGCACC ATGAACTACC CCACCATCCT ACCTTCAGGA 
GCAGTCAACA GCAAAGGCAG TACCAGCTCG CGACCCAAGG GGCTTCGGAG TGCTGGCACC 
CCAGGGGCCA CAGCACACAG CCCTCTGAGC CTGGGGAGCC CCAGCCCCTA TGAGAAGATC 
CAGCACGTGG TTGGAGAACA AGGACTAGGC CGAGCCTCTA GTCACCTGTC AGGCCTGAGT 
GTGCCTTGCC CCCTGCCCAG CCCCCAGGCT GGCACACTGA CCTGTGAGCT GAAGAGCTGC 
CCATATTGTG CCAGCGCCCT GGAGGACCCC GAGTTTGAGT TCAGTGGCTC AGAGAGTGGG 
GACTCGGATG CCCATGGAGT CTATGAGTTT ACACAGGACG TACGGCATGG GGATTGCCGA 
GATCCTGTGC AGCAGCCCCA TGAAGGGGGC ACGCCGGGCC ATGGCAACGA ACGGTGGCGG 
CCACCACTGC GGACAGCCTC ACAGCCAGGA GGGTTAGGCC GCCTCTGGGC TTCCTTTAGT 
AGCAAGCTGC GTCGCATTGT AGACAGCAAG TACTTCAACC GAGGCATCAT GGCAGCCATC 
CTCGTCAATA CTCTGAGCAT GGGTGTTGAG TATCATGAGC AGCCTGATGA GCTGACTAAC 
GCGCTGGAGA TAAGCAACAT CGTGTTCACC AGCATGTTTG CCTTGGAGAT GCTACTGAAG 
TTGCTGGCCT GTGGCCCGCT GGGCTACATT CGGAACCCCT ACAACATCTT TGATGGCATT 
GTTGTCATCA TAAGCGTCTG GGAGATTGTG GGGCAGGCAG ATGGTGGCCT GTCAGTGCTC 
CGTACGTTCC GGCTGCTGCG GGTGCTGAAG CTGGTGCGCT TCCTGCCAGC CCTGCGGCGG 
CAGCTGGTGG TGCTCATGAG GACCATGGAC AATGTGGCCA CCTTCTGCAT GCTGCTAATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTGT TTGGCTGTAA GTTCAGCCTG 
AAGACAGACT CTGGAGACAC CGTCCCTGAC AGGAAGAACT TCGACTCTCT ACTGTGGGCC 
ATCGTCACTG TGTTTCAGAT CTTGACACAG GAAGACTGGA ATGTGGTTCT TTACAACGGC 
ATGGCTTCCA CCTCGTCCTG GGCTGCCCTT TACTTTGTGG CCCTCATGAC CTTTGGGAAC 
TATGTGCTCT TCAACCTGCT GGTAGCCATC CTGGTGGAAG GCTTCCAGGC AGAGGGTGAT 
GCCACCAGAT CTGACACCGA TGAGGATAAG ACATCTACCC ACCTAGAGGA AGATTTCGAT 
AAGCTCAGAG ACGTTCAAGC CACAGAGATG AAGATGTACT CACTGGCTGT GACCCCTAAT 
GGGCACCTAG AGGGCCGAGG CAGTCTGCCG CCGCCTCTCA TCACGCACAC AGCGGCTACG 
CCTATGCCTA CCCCCAAGAG CTCCCCTCAT CTGGACATGG CCCACACTCT TTTGGACTCA 
CGGCGCAGCA GCAGTGGCTC TGTGGACCCC CAACTCGGGG ACCAGAAGTC TCTGGCTAGC 
CTGCGCAGCT CCCCTTGTGC CCCATGGGGC CCCAACAGTG CAGGAAGCAG CCGGCGCTCC 
AGCTGGAACA GCCTGGGCCG CGCACCCAGC CTCAAACGCC GCAGCCAGTG TGGGGAGCGC 
GAGTCCCTGC TCTCTGGTGA GGGGAAGGGC AGCACGGATG ATGAGGCCGA GGACAGCAGA 
CCAAACTCAG GGACCCACCC AGGAGCTTCA CCCGGGCCCC GGGCCACACC CCTGCGGCGG 
GCTGAGTCGT TGGGCCACCG CAGCACAATG GACCTGTGCC CCCCACGGCC TGCCACCCTC 
CTGCCCACCA AGTTCCGTGA CTGCAACGGG CAGATGGTGG CCCTGCCCAG CGAGTTCTTC 
CTGCGCATCG ACAGCCACAA GGAGGACGCA GCAGAGTTTG ATGATGACAT AGAAGATAGC 
TGCTGCTTCC GCCTGCACAA AGTGCTGGAG CCCTATGCAC CCCAGTGGTG CAGCAGCCGG 
GAGTCCTGGG CCCTGTATCT CTTCCCGCCG CAGAACAGGC TACGCGTCTC CTGCCAGAAA 
GTCATCGCAC ACAAGATGTT TGACCACGTG GTCCTTGTCT TCATCTTCCT CAACTGTATC 
ACCATTGCCC TGGAGAGGCC AGACATCGAT CCGGGCAGCA CTGAACGGGC CTTTCTCAGC 
GTCTCCAACT ACATCTTCAC AGCCATCTTC GTGGTGGAGA TGATGGTGAA GGTGGTAGCC 
CTGGGGCTGC TGTGGGGTGA GCATGCCTAC CTGCAGAGCA GCTGGAATGT GCTGGACGGG 
CTGCTTGTCC TGGTGTCCCT GGTTGACATC ATTGTGGCCG TGGCCTCTGC CGGTGGTGCC 
AAGATCCTAG GCGTCCTGCG CGTGCTGCGC CTGCTGCGGA CCCTGAGGCC TCTGAGGGTC 
ATCAGCCGAG CTCCAGGCCT CAAGCTGGTC GTGGAGACTC TGATATCATC GCTCAGGCCC 
ATCGGGAACA TCGTCCTCAT CTGCTGCGCC TTCTTCATTA TCTTTGGCAT CCTCGGGGTG 
CAGCTTTTCA AGGGCAAATT CTACTACTGC GAGGGTACAG ATACCAGGAA TATCACCACC 
AAGGCCGAGT GCCATGCCGC CCACTACCGC TGGGTGAGGC GCAAATACAA CTTTGACAAC 
CTGGGTCAGG CGCTGATGTC TCTGTTCGTG CTGTCATCCA AGGATGGCTG GGTAAACATC 
ATGTACGACG GGCTGGACGC CGTGGGCATC GACCAGCAGC CTGTGCAGAA CCACAACCCC 
TGGATGCTGC TCTACTTCAT CTCCTTCCTG CTCATCGTCA GCTTCTTCGT GCTCAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAC AAGTGCCGGC AGCACCAGGA GGCGGAGGAA 
GCGCGGCGGC GCGAGGAGAA ACGGCTGCGG CGCCTGGAGA GGAGGCGCAG GAGCACTTTC 
CCCAACCCAG AGGCCCAGCG CCGGCCCTAC TACGCAGACT ATTCACACAC GCGCCGCTCC 
ATCCATTCGC TGTGCACCAG CCACTACCTG GACCTCTTCA TCACCTTCAT CATCTGCCTC 
AATGTCATCA CCATGTCCAT GGAGCACTAC AACCAGCCCA AGTCTCTGGA CGAGGCCCTT 
AAGTACTGCA ACTACGTCTT TACCATCGTC TTCGTCTTTG AGGCCGCACT GAAGCTGGTG 
GCCTTTGGAT TCCGGAGGTT CTTCAAGGAC AGGTGGAACC AGCTGGACTT GGCCATCGTC 
CTCCTGTCCA TCATGGGCAT TGCGCTGGAG GAGATTGAGA TGAATGCTGC CCTCCCCATC 
AACCCCACCA TCATCCGCAT CATGCGCGTG CTTCGCATCG CCCGTGTGCT GAAGCTACTG 
AAGATGGCCA CAGGCATGCG CGCCCTGCTG GACACTGTGG TTCAAGCTCT GCCTCAGGTA 
GGGAACCTTG GTCTTCTTTT CATGCTCCTG TTTTTTATCT ATGCTGCCCT GGGAGTGGAG 
CTGTTTGGGA GGCTAGAATG CAGCGAGGAT AATCCCTGTG AGGGCCTGAG CAGACACGCC 
ACCTTCACCA ACTTTGGCAT GGCCTTCCTC ACGTTGTTCC GAGTGTCCAC TGGGGACAAC 
TGGAATGGGA TCATGAAGGA TACTCTCCGT GAATGTACCC GTGAAGACAA GCACTGCCTC 
AGCTACCTGC CCGCGCTCTC CCCCGTCTAC TTCGTCACCT TCGTGCTGGT CGCCCAGTTC 
GTGCTGGTCA ACGTGGTGGT GGCCGTGCTC ATGAAGCACC TGGAGGAGAG CAACAAGGAG 
GCCCGGGAGG ATGCGGAGAT GGATGCCGAG ATCGAGCTGG AGATCGCACA GGGGTCCACA 
GCCCAGCCCC CGTCCACAGC ACAGGAAAGC CAAGGTACCG AGCCAGACAC CCCGAACCTC 
CTGGTCGTGC GCAAAGTATC TGTGTCCAGG ATGCTCTCGC TACCCAATGA CAGCTACATG 
TTCCGGCCGG TGGCTCCTGC GGCTGCCCCG CATTCCCACC CACTGCAGGA AGTGGAGATG 
GAGACCTACA CAGGCCCGGT CACCTCTGCT CACTCGCCAT CCCTGGAGCC CCGCACCTCC 
TTCCAGGTCC CATCAGCTGC GTCCTCCCCA GCCAGGGTCA GCGACCCCCT TTGTGCCCTC 
TCACCCCGGG ATACGCCCCG CTCTCTGAGC CTCTCACGGA TACTCTGCAG ACAGGAGGCC 
ATGCACGCAG AGTCCCTGGA AGGGCAGATA GATGACGCTG GAGAAGACAG CATCCCAGAC 
TACACGGAGC CTGCTGAAAA TATCTCCATG AGCCAGGCAC CTCTGGGTAC CCTGCGGTCT 
CCTCCATGCT CCCCACGGCC TGCCAGTGTC CGTACCCGCA AGCACACTTT TGGGCAGCAC 
TGCATCTCCA GCCGCCCTCC CACCCTGGGA GGAGATGATG CTGAAGCAGC AGACCCGGCC 
GATGAGGAGG TCAGCCACAT CACCAGCTCA GCCCACCCTT GGCCAGCTAC AGAGCCCCAC 
AGCCCAGAGG CCTCCCCGAC AGCCTCTCCT GCAAAAGGCA CAGTGGGCAG TGGGCGGGAC 
CCACACAGGT TCTGCAGTGT AGATGCTCAG AGCTTCCTGG ACAAACCAGG TCGGCCAGAT 
GCACAGCGGT GGTCCTCGGT GGAACTGGAT AATGGGGATG GCCACCTAGA GTCTGGGGAA 
GTGAGGGCCC GGGCCTCAGA GCTAGAACCA GCTCTTGGGG CACGGAGGAA GAAGAAGATG 
AGCCCTCCCT GCATCTCCAT TGACCCTCCC ACTGAAGATG AGGGCTCCTC CCGCCCCCCT 
GCAGCAGAAG GAGGCAACAC TACTCTGAGG CGCCGAACCC CGTCCTGTGA GGCTGCCCTC 
CATAGGGACT GCCCAGAGTC TACAGAGGGC CCAGGCACTG GAGGGGACCC TGTAGCCAAG 
GGTGAGCGCT GGGGCCAGGC CTCTTGCCGG GCAGAGCATC TGACTGTCCC CAACTTTGCC 
TTTGAGCCTC TGGACATGGG CGGACCTGGT GGAGACTGTT TCTTGGACAG TGACCAAAGT 
GTGACCCCAG AACCCAGAGT TTCCTCTTTG GGGGCTATAG TGCCTCTAAT ACTAGAAACT 
GAACTTTCTA TGCCCTCTGG TGACCCCCCA GAGAAGGAGC AAGGACTGTA CCTCACTGTG 
CCCCAGACCC CCTTGAAGAA ACCAGGGTCT CCCCCAGCCA CTCCTGCCCC AGATGACAGT 
GGAGATGAGC CTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The T-type Ca
Neuroscience40278-89(2019 03)
Rebellato P,Kaczynska D,Kanatani S,Rayyes IA,Zhang S,Villaescusa C,Falk A,Arenas E,Hermanson O,Louhivuori L,Uhlén P

Cav3.2 calcium channel interactions with the epithelial sodium channel ENaC.
Molecular brain12(1)12(2019 02)
Garcia-Caballero A,Gandini MA,Huang S,Chen L,Souza IA,Dang YL,Stutts MJ,Zamponi GW

Cav3.2 T-type calcium channels shape electrical firing in mouse Lamina II neurons.
Scientific reports9(1)3112(2019 Feb)
Candelas M,Reynders A,Arango-Lievano M,Neumayer C,Fruquière A,Demes E,Hamid J,Lemmers C,Bernat C,Monteil A,Compan V,Laffray S,Inquimbert P,Le Feuvre Y,Zamponi GW,Moqrich A,Bourinet E,Méry PF

Caveolae Link Ca
Arteriosclerosis, thrombosis, and vascular biology38(10)2371-2381(2018 10)
Hashad AM,Harraz OF,Brett SE,Romero M,Kassmann M,Puglisi JL,Wilson SM,Gollasch M,Welsh DG

Disrupting USP5/Cav3.2 interactions protects female mice from mechanical hypersensitivity during peripheral inflammation.
Molecular brain11(1)60(2018 10)
Gadotti VM,Zamponi GW

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Cacna1h cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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