CACNA1H cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1H
Entrez Gene ID	416526
Full Name	calcium voltage-gated channel subunit alpha1 H
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 H Names voltage-dependent T-type calcium channel subunit alpha-1H calcium channel, voltage-dependent, T type, alpha 1H subunit
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015294423.1	XP_015149909.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
XM_015294423.2	XP_015149909.2	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
XM_015294424.2	XP_015149910.2	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
XM_015294424.1	XP_015149910.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
XM_015294422.1	XP_015149908.1	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
XM_015294422.2	XP_015149908.2	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC560875	XP_003198197.2
Homo sapiens (human)	CACNA1H	NP_066921.2
Bos taurus (cattle)	CACNA1H	XP_002698020.3
Mus musculus (house mouse)	Cacna1h	NP_067390.4
Rattus norvegicus (Norway rat)	Cacna1h	NP_722521.1
Gallus gallus (chicken)	CACNA1H	XP_414830.4
Xenopus tropicalis (tropical clawed frog)	LOC100494894	XP_002932520.2
Pan troglodytes (chimpanzee)	CACNA1H	XP_001172468.2
Canis lupus familiaris (dog)	CACNA1H	XP_537016.2

Gene Ontology

The following CACNA1H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa144531	XM_015294422.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa144532	XM_015294423.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa144533	XM_015294424.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa42352	XM_015294422.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa42353	XM_015294423.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa42354	XM_015294424.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1H ( XM_015294422.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149908.2 Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X1, mRNA.

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CloneID	OGa144531
Clone ID Related Accession (Same CDS sequence)	XM_015294422.2
Accession Version	XM_015294422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTTTCTGAAA CAAAGCAGCG GGAGAACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTGAGCAT GGGCATCGAG TACCACGAAC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTGCTCCG GGTGCTGAAG TTGGTGCGCT TCATGCCCGC CCTCCGTCGC 
CAGCTGGTGG TCCTGATGAA GACAATGGAC AACGTTGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTGGTGGTG GAGAACTTTC ACAAGTGCCG GCAGCACCAG 
GAGGCAGAGG AGGCCCGGCG TCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGAGG CGCTGAGCTG TGAGAATGTG TCTGTAGCCA TCCCAGACAC GCTGGAGGGG 
GCTGAGACTC CCATGATGGA AATCCTGTCC TTCTGCTCTG TTTCCCACCA TGTCCTGACA 
CCTTCCCACC ACCCCCGAGG CCGCGAGGCT GAGGGAGGCA CGGCCATCCC GCAGCCTCAG 
GACTCCCAGA ACATGCTGAC GGTCCGCAAG ATCTCCGTTT CACGCATGCA CTCCTTGCCC 
AACGACAGCT ACATGTTCCG GCCCGTGATG CCAGCCAAAG CTCCCTTTCC CCTCCACGAG 
GTGGAGATGG AGGCATATCC CTGCAAGTCA CAGCGAGGGT CCATCACCTC CATCCGCTCG 
CAGCCCGTGG GGACGTGCTC CTCCTTGAGG GTCCCCCCCG AGTCCCTCCA CGTGGCCGTG 
CGCTCCCCCG GCCGTGAGGG CTGGCACCGC TGGAAGGTCC TTCCCCCGCA CGTCGTCCCT 
CGGTCTCCTA CTCTCAGCAA GCTGCTGTGC CGGCAGGAGG CCGTTCGGAC AGACTCGGTG 
GACGGACACC CTCCAGACCC CAAGGACAAC CTGCAAGCAG AGCGTGGAGA GATGCCACCA 
TCACCAAAGC ACCCTCAGCG CACCCTCACT CCCAGCCCTC TCCACCCTGC AGCCTCCTCC 
CTGCCGGGCT CCCCTCCGCG CTCCCCACCA ACCTCCCCAC ACCGGCACCC ATCGCTGACT 
CGGAAACACC CCTGCAGCCA GCGCGGGGTC CCCAGTCCCA CCGAGAGCCC CCCAAGCCCC 
CAGGGCTGCC TCCTGGAGCC CTCTGACCTG GCTGACGAGG AGGTTCGCCA CATCAACAGC 
TCAGCCAAAG ACTGGGGGGA GCACTCGGAG GCCGGGAGCT GCTCCACGTC GCCGTTCGTC 
TCGCCGGCCG CGTCGCCGGT GCCCCTGAAG AATTGCAGCA CAAGCAGCCT GACGGCAGGC 
AGCGGCAAGG AGAGGGACTT GAAGAAGTTT TACAGCATCG ATACGAAGGG CTTCCTCACC 
AAGCCCAGCT GGGCGGATGA CCAAAGGCGG CACTCCATCG AGATCTGCCC CTCCGTCCGC 
GATGGGGACT GCGGCTTCGA GGAGCGCGCC GAGGAAAAGC CACGCTCCCC CGCCCACGTC 
CAGCCAGAGT CCGAGTACAT CCACGGAGCC CGGAGGAAGA AGAAGATGAG CCCGCCCTGC 
ATTTCTATAG ATCCTCCCAT GGAGGACGAC AGCGGGGCGG CCTCGCGCAC CAAACCCTCC 
GAGAACAGCA TGCTGCGCCG CAGGACCCCG TCCTGCGAGT TCCCGGCGTA CCGAGAGCCC 
CTGGAGCCGG CGGAGGCGCA GCCGGGGGAG CCGGGCTGCA AAGCAGAGCG CCGTGCCCAG 
CACCTCGCCA TCCCCGGCTT CTCCTTCGAG CAGTCGGATG GCGGCGGCTC CCTGAGCAGC 
CTCTCAGACC TGCTGGACAG TGGGCGATCC ACACCGGACT GCGAACTGAG AAAGCCGGAG 
CCCTTAAAAA CTCAACGGAG CAACGCCGAG AAAAGCAAAG AGCTGCTCAG CGTGGCCAAG 
AGCCCCGTCC GGATGCAGGA CTTGGTGACT GTTGCAACAG AAGAAGACAT CGACGAGCCC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149908.2
CDS	374..7759
Translation	MSDGESREPRPAGGEVRVPITVPPRRAAEQPLDDAGSAPSGSPSRDPPPDLASEEEEQVPYPALAPTAFFCLKQTTRPRSWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDVECQSERCTILEAFDDFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDRNLATTYNLTFLHPYYRTDEAEENPFICSSHRENGMQKCSNIPTRKEYKVECTLSMDSYTPSLYNPDFSSKNACINWNQYYNVCMAGDVNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTIRLYNNWQSKRRKKVNPNNTTNGQSRRGKKRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSNPEICDLELKVMKPGGQLMLPSPSPNLQSPVPPPDSESVHSIYHADCHVEGPQVNCKSSNAPASIKLTTGLSNHGNMNYPTILPSPTSKASSVPVPKGKKNGSSPVAVVNSPVTLGTDSYGKLQQLVGEHGLRRTPSRLSGLSVACPLPSPPGGTLSCELQDCPFCASLLEDPEFAFSESDSCDSDSNGIYEFTQDLRHGDHRDQLQQQRGRRKRKKKKPKERNKVTRLWKAFGSKLKRIVESKYFNRGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSMFALEMLLKLLAFGLFGYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSANFDDDFEKLKDLRATEMKMYSLAVTPNGHLEGRGSMPPPIIMRTAATPMPTPKCSPHMDSVHTFVDSRRGSNASLDPLTYDQKSSSSLRSSPCANWGTNSNWGSRRSSWNSLGRAPSLKKKNQSGERESLLSGEGKGSTDDDSDDAKSSTVSRPSLHRRAESLDYRSSLDLPELLQLPPMRHSLSISPMAVLPAEYQDCNGKMVHVPSEFFLHIDGHKEEAVDYEDDMEDSYCYRIRKVLEPYKPEWCKSHEDWSLYLFSPQNRFRVMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPHSTERIFLSVSNYIFTAIFVAEMMVKVVALGFFSGENTYLQSSWNVLDGVLVFVSIIDIIVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCDGPDVKNITTKTDCTNAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPIQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKEMYLSEAQRRPYYADYSPARKYIHTLCTSHYLDLFITFIIGVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSIVGITLEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCSEENPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIELEMSRGASTTSGSRGGAVALESRASCRSQEAMKSEPAAQAKHQALEALSCENVSVAIPDTLEGAETPMMEILSFCSVSHHVLTPSHHPRGREAEGGTAIPQPQDSQNMLTVRKISVSRMHSLPNDSYMFRPVMPAKAPFPLHEVEMEAYPCKSQRGSITSIRSQPVGTCSSLRVPPESLHVAVRSPGREGWHRWKVLPPHVVPRSPTLSKLLCRQEAVRTDSVDGHPPDPKDNLQAERGEMPPSPKHPQRTLTPSPLHPAASSLPGSPPRSPPTSPHRHPSLTRKHPCSQRGVPSPTESPPSPQGCLLEPSDLADEEVRHINSSAKDWGEHSEAGSCSTSPFVSPAASPVPLKNCSTSSLTAGSGKERDLKKFYSIDTKGFLTKPSWADDQRRHSIEICPSVRDGDCGFEERAEEKPRSPAHVQPESEYIHGARRKKKMSPPCISIDPPMEDDSGAASRTKPSENSMLRRRTPSCEFPAYREPLEPAEAQPGEPGCKAERRAQHLAIPGFSFEQSDGGGSLSSLSDLLDSGRSTPDCELRKPEPLKTQRSNAEKSKELLSVAKSPVRMQDLVTVATEEDIDEPV

Target ORF information:

RefSeq Version	XM_015294422.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294422.2

ORF Insert Sequence:

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTTTCTGAAA CAAAGCAGCG GGAGAACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTGAGCAT GGGCATCGAG TACCACGAAC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTGCTCCG GGTGCTGAAG TTGGTGCGCT TCATGCCCGC CCTCCGTCGC 
CAGCTGGTGG TCCTGATGAA GACAATGGAC AACGTTGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTGGTGGTG GAGAACTTTC ACAAGTGCCG GCAGCACCAG 
GAGGCAGAGG AGGCCCGGCG TCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGAGG CGCTGAGCTG TGAGAATGTG TCTGTAGCCA TCCCAGACAC GCTGGAGGGG 
GCTGAGACTC CCATGATGGA AATCCTGTCC TTCTGCTCTG TTTCCCACCA TGTCCTGACA 
CCTTCCCACC ACCCCCGAGG CCGCGAGGCT GAGGGAGGCA CGGCCATCCC GCAGCCTCAG 
GACTCCCAGA ACATGCTGAC GGTCCGCAAG ATCTCCGTTT CACGCATGCA CTCCTTGCCC 
AACGACAGCT ACATGTTCCG GCCCGTGATG CCAGCCAAAG CTCCCTTTCC CCTCCACGAG 
GTGGAGATGG AGGCATATCC CTGCAAGTCA CAGCGAGGGT CCATCACCTC CATCCGCTCG 
CAGCCCGTGG GGACGTGCTC CTCCTTGAGG GTCCCCCCCG AGTCCCTCCA CGTGGCCGTG 
CGCTCCCCCG GCCGTGAGGG CTGGCACCGC TGGAAGGTCC TTCCCCCGCA CGTCGTCCCT 
CGGTCTCCTA CTCTCAGCAA GCTGCTGTGC CGGCAGGAGG CCGTTCGGAC AGACTCGGTG 
GACGGACACC CTCCAGACCC CAAGGACAAC CTGCAAGCAG AGCGTGGAGA GATGCCACCA 
TCACCAAAGC ACCCTCAGCG CACCCTCACT CCCAGCCCTC TCCACCCTGC AGCCTCCTCC 
CTGCCGGGCT CCCCTCCGCG CTCCCCACCA ACCTCCCCAC ACCGGCACCC ATCGCTGACT 
CGGAAACACC CCTGCAGCCA GCGCGGGGTC CCCAGTCCCA CCGAGAGCCC CCCAAGCCCC 
CAGGGCTGCC TCCTGGAGCC CTCTGACCTG GCTGACGAGG AGGTTCGCCA CATCAACAGC 
TCAGCCAAAG ACTGGGGGGA GCACTCGGAG GCCGGGAGCT GCTCCACGTC GCCGTTCGTC 
TCGCCGGCCG CGTCGCCGGT GCCCCTGAAG AATTGCAGCA CAAGCAGCCT GACGGCAGGC 
AGCGGCAAGG AGAGGGACTT GAAGAAGTTT TACAGCATCG ATACGAAGGG CTTCCTCACC 
AAGCCCAGCT GGGCGGATGA CCAAAGGCGG CACTCCATCG AGATCTGCCC CTCCGTCCGC 
GATGGGGACT GCGGCTTCGA GGAGCGCGCC GAGGAAAAGC CACGCTCCCC CGCCCACGTC 
CAGCCAGAGT CCGAGTACAT CCACGGAGCC CGGAGGAAGA AGAAGATGAG CCCGCCCTGC 
ATTTCTATAG ATCCTCCCAT GGAGGACGAC AGCGGGGCGG CCTCGCGCAC CAAACCCTCC 
GAGAACAGCA TGCTGCGCCG CAGGACCCCG TCCTGCGAGT TCCCGGCGTA CCGAGAGCCC 
CTGGAGCCGG CGGAGGCGCA GCCGGGGGAG CCGGGCTGCA AAGCAGAGCG CCGTGCCCAG 
CACCTCGCCA TCCCCGGCTT CTCCTTCGAG CAGTCGGATG GCGGCGGCTC CCTGAGCAGC 
CTCTCAGACC TGCTGGACAG TGGGCGATCC ACACCGGACT GCGAACTGAG AAAGCCGGAG 
CCCTTAAAAA CTCAACGGAG CAACGCCGAG AAAAGCAAAG AGCTGCTCAG CGTGGCCAAG 
AGCCCCGTCC GGATGCAGGA CTTGGTGACT GTTGCAACAG AAGAAGACAT CGACGAGCCC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1H ( XM_015294423.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149909.2 Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X2, mRNA.

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CloneID	OGa144532
Clone ID Related Accession (Same CDS sequence)	XM_015294423.2
Accession Version	XM_015294423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTTTCTGAAA CAAAGCAGCG GGAGAACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTGAGCAT GGGCATCGAG TACCACGAAC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTGCTCCG GGTGCTGAAG TTGGTGCGCT TCATGCCCGC CCTCCGTCGC 
CAGCTGGTGG TCCTGATGAA GACAATGGAC AACGTTGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTGGTGGTG GAGAACTTTC ACAAGTGCCG GCAGCACCAG 
GAGGCAGAGG AGGCCCGGCG TCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAAAGCCC AGCGCAGGCC TTACTATGCT GACTATTCTC CAGCACGGAA ATACATTCAC 
ACCCTCTGCA CCAGCCACTA TCTCGACCTC TTCATCACCT TCATCATTGG AGTCAACGTC 
ATCACTATGT CGATGGAGCA CTACAACCAG CCAAAGTCCC TGGATGAAGC CCTGAAGTAC 
TGTAACTACG TGTTCACCAT AGTTTTTGTG TTCGAGGCAG TCCTGAAGTT GGTGGCATTT 
GGTTTTCGAA GGTTCTTTAA GGACAGGTGG AATCAGCTGG ATTTGGCCAT CGTTCTCTTA 
TCCATTGTGG GAATCACGCT GGAGGAAATT GAGATGAACG CTGCACTGCC CATCAATCCC 
ACAATAATAC GCATCATGCG GGTGCTGAGA ATAGCAAGAG TGCTGAAACT GCTTAAAATG 
GCCACCGGAA TGCGAGCCCT CCTGGACACA GTGGTACAAG CGCTTCCCCA GGTAGGGAAC 
CTTGGCCTAC TTTTTATGCT CCTGTTTTTT ATCTATGCTG CCCTGGGAGT GGAATTGTTT 
GGCAAGCTAG ACTGCAGCGA AGAGAATCCT TGCGAAGGGC TGAGCCGGCA CGCCACGTTC 
ACCAACTTTG GGATGGCCTT CCTCACCCTC TTCAGGGTGT CCACGGGGGA CAACTGGAAC 
GGTATCATGA AGGACACTCT CCGGGAATGC ACGCGGGAAG ACAAGCACTG CCTCAGCTAC 
CTGCCTGTTA TCTCTCCTGT CTACTTCGTC ACCTTCGTCC TCATCGCACA GTTCGTCCTG 
GTCAACGTGG TGGTGGCCGT GCTGATGAAG CACTTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGACGCTG AGATGGACGC TGAGATCGAG CTGGAGATGT CCCGAGGAGC GAGCACCACC 
AGTGGCAGCA GGGGAGGTGC GGTGGCCCTG GAGAGCAGAG CATCCTGCAG AAGCCAGGAG 
GCCATGAAGT CAGAGCCAGC AGCGCAGGCG AAGCACCAAG CCCTGGAGGC GCTGAGCTGT 
GAGAATGTGT CTGTAGCCAT CCCAGACACG CTGGAGGGGG CTGAGACTCC CATGATGGAA 
ATCCTGTCCT TCTGCTCTGT TTCCCACCAT GTCCTGACAC CTTCCCACCA CCCCCGAGGC 
CGCGAGGCTG AGGGAGGCAC GGCCATCCCG CAGCCTCAGG ACTCCCAGAA CATGCTGACG 
GTCCGCAAGA TCTCCGTTTC ACGCATGCAC TCCTTGCCCA ACGACAGCTA CATGTTCCGG 
CCCGTGATGC CAGCCAAAGC TCCCTTTCCC CTCCACGAGG TGGAGATGGA GGCATATCCC 
TGCAAGTCAC AGCGAGGGTC CATCACCTCC ATCCGCTCGC AGCCCGTGGG GACGTGCTCC 
TCCTTGAGGG TCCCCCCCGA GTCCCTCCAC GTGGCCGTGC GCTCCCCCGG CCGTGAGGGC 
TGGCACCGCT GGAAGGTCCT TCCCCCGCAC GTCGTCCCTC GGTCTCCTAC TCTCAGCAAG 
CTGCTGTGCC GGCAGGAGGC CGTTCGGACA GACTCGGTGG ACGGACACCC TCCAGACCCC 
AAGGACAACC TGCAAGCAGA GCGTGGAGAG ATGCCACCAT CACCAAAGCA CCCTCAGCGC 
ACCCTCACTC CCAGCCCTCT CCACCCTGCA GCCTCCTCCC TGCCGGGCTC CCCTCCGCGC 
TCCCCACCAA CCTCCCCACA CCGGCACCCA TCGCTGACTC GGAAACACCC CTGCAGCCAG 
CGCGGGGTCC CCAGTCCCAC CGAGAGCCCC CCAAGCCCCC AGGGCTGCCT CCTGGAGCCC 
TCTGACCTGG CTGACGAGGA GGTTCGCCAC ATCAACAGCT CAGCCAAAGA CTGGGGGGAG 
CACTCGGAGG CCGGGAGCTG CTCCACGTCG CCGTTCGTCT CGCCGGCCGC GTCGCCGGTG 
CCCCTGAAGA ATTGCAGCAC AAGCAGCCTG ACGGCAGGCA GCGGCAAGGA GAGGGACTTG 
AAGAAGTTTT ACAGCATCGA TACGAAGGGC TTCCTCACCA AGCCCAGCTG GGCGGATGAC 
CAAAGGCGGC ACTCCATCGA GATCTGCCCC TCCGTCCGCG ATGGGGACTG CGGCTTCGAG 
GAGCGCGCCG AGGAAAAGCC ACGCTCCCCC GCCCACGTCC AGCCAGAGTC CGAGTACATC 
CACGGAGCCC GGAGGAAGAA GAAGATGAGC CCGCCCTGCA TTTCTATAGA TCCTCCCATG 
GAGGACGACA GCGGGGCGGC CTCGCGCACC AAACCCTCCG AGAACAGCAT GCTGCGCCGC 
AGGACCCCGT CCTGCGAGTT CCCGGCGTAC CGAGAGCCCC TGGAGCCGGC GGAGGCGCAG 
CCGGGGGAGC CGGGCTGCAA AGCAGAGCGC CGTGCCCAGC ACCTCGCCAT CCCCGGCTTC 
TCCTTCGAGC AGTCGGATGG CGGCGGCTCC CTGAGCAGCC TCTCAGACCT GCTGGACAGT 
GGGCGATCCA CACCGGACTG CGAACTGAGA AAGCCGGAGC CCTTAAAAAC TCAACGGAGC 
AACGCCGAGA AAAGCAAAGA GCTGCTCAGC GTGGCCAAGA GCCCCGTCCG GATGCAGGAC 
TTGGTGACTG TTGCAACAGA AGAAGACATC GACGAGCCCG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149909.2
CDS	374..7738
Translation	MSDGESREPRPAGGEVRVPITVPPRRAAEQPLDDAGSAPSGSPSRDPPPDLASEEEEQVPYPALAPTAFFCLKQTTRPRSWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDVECQSERCTILEAFDDFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDRNLATTYNLTFLHPYYRTDEAEENPFICSSHRENGMQKCSNIPTRKEYKVECTLSMDSYTPSLYNPDFSSKNACINWNQYYNVCMAGDVNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTIRLYNNWQSKRRKKVNPNNTTNGQSRRGKKRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSNPEICDLELKVMKPGGQLMLPSPSPNLQSPVPPPDSESVHSIYHADCHVEGPQVNCKSSNAPASIKLTTGLSNHGNMNYPTILPSPTSKASSVPVPKGKKNGSSPVAVVNSPVTLGTDSYGKLQQLVGEHGLRRTPSRLSGLSVACPLPSPPGGTLSCELQDCPFCASLLEDPEFAFSESDSCDSDSNGIYEFTQDLRHGDHRDQLQQQRGRRKRKKKKPKERNKVTRLWKAFGSKLKRIVESKYFNRGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSMFALEMLLKLLAFGLFGYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSANFDDDFEKLKDLRATEMKMYSLAVTPNGHLEGRGSMPPPIIMRTAATPMPTPKCSPHMDSVHTFVDSRRGSNASLDPLTYDQKSSSSLRSSPCANWGTNSNWGSRRSSWNSLGRAPSLKKKNQSGERESLLSGEGKGSTDDDSDDAKSSTVSRPSLHRRAESLDYRSSLDLPELLQLPPMRHSLSISPMAVLPAEYQDCNGKMVHVPSEFFLHIDGHKEEAVDYEDDMEDSYCYRIRKVLEPYKPEWCKSHEDWSLYLFSPQNRFRVMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPHSTERIFLSVSNYIFTAIFVAEMMVKVVALGFFSGENTYLQSSWNVLDGVLVFVSIIDIIVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCDGPDVKNITTKTDCTNAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPIQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRRPYYADYSPARKYIHTLCTSHYLDLFITFIIGVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSIVGITLEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCSEENPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIELEMSRGASTTSGSRGGAVALESRASCRSQEAMKSEPAAQAKHQALEALSCENVSVAIPDTLEGAETPMMEILSFCSVSHHVLTPSHHPRGREAEGGTAIPQPQDSQNMLTVRKISVSRMHSLPNDSYMFRPVMPAKAPFPLHEVEMEAYPCKSQRGSITSIRSQPVGTCSSLRVPPESLHVAVRSPGREGWHRWKVLPPHVVPRSPTLSKLLCRQEAVRTDSVDGHPPDPKDNLQAERGEMPPSPKHPQRTLTPSPLHPAASSLPGSPPRSPPTSPHRHPSLTRKHPCSQRGVPSPTESPPSPQGCLLEPSDLADEEVRHINSSAKDWGEHSEAGSCSTSPFVSPAASPVPLKNCSTSSLTAGSGKERDLKKFYSIDTKGFLTKPSWADDQRRHSIEICPSVRDGDCGFEERAEEKPRSPAHVQPESEYIHGARRKKKMSPPCISIDPPMEDDSGAASRTKPSENSMLRRRTPSCEFPAYREPLEPAEAQPGEPGCKAERRAQHLAIPGFSFEQSDGGGSLSSLSDLLDSGRSTPDCELRKPEPLKTQRSNAEKSKELLSVAKSPVRMQDLVTVATEEDIDEPV

Target ORF information:

RefSeq Version	XM_015294423.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294423.2

ORF Insert Sequence:

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTTTCTGAAA CAAAGCAGCG GGAGAACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTGAGCAT GGGCATCGAG TACCACGAAC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTGCTCCG GGTGCTGAAG TTGGTGCGCT TCATGCCCGC CCTCCGTCGC 
CAGCTGGTGG TCCTGATGAA GACAATGGAC AACGTTGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTGGTGGTG GAGAACTTTC ACAAGTGCCG GCAGCACCAG 
GAGGCAGAGG AGGCCCGGCG TCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAAAGCCC AGCGCAGGCC TTACTATGCT GACTATTCTC CAGCACGGAA ATACATTCAC 
ACCCTCTGCA CCAGCCACTA TCTCGACCTC TTCATCACCT TCATCATTGG AGTCAACGTC 
ATCACTATGT CGATGGAGCA CTACAACCAG CCAAAGTCCC TGGATGAAGC CCTGAAGTAC 
TGTAACTACG TGTTCACCAT AGTTTTTGTG TTCGAGGCAG TCCTGAAGTT GGTGGCATTT 
GGTTTTCGAA GGTTCTTTAA GGACAGGTGG AATCAGCTGG ATTTGGCCAT CGTTCTCTTA 
TCCATTGTGG GAATCACGCT GGAGGAAATT GAGATGAACG CTGCACTGCC CATCAATCCC 
ACAATAATAC GCATCATGCG GGTGCTGAGA ATAGCAAGAG TGCTGAAACT GCTTAAAATG 
GCCACCGGAA TGCGAGCCCT CCTGGACACA GTGGTACAAG CGCTTCCCCA GGTAGGGAAC 
CTTGGCCTAC TTTTTATGCT CCTGTTTTTT ATCTATGCTG CCCTGGGAGT GGAATTGTTT 
GGCAAGCTAG ACTGCAGCGA AGAGAATCCT TGCGAAGGGC TGAGCCGGCA CGCCACGTTC 
ACCAACTTTG GGATGGCCTT CCTCACCCTC TTCAGGGTGT CCACGGGGGA CAACTGGAAC 
GGTATCATGA AGGACACTCT CCGGGAATGC ACGCGGGAAG ACAAGCACTG CCTCAGCTAC 
CTGCCTGTTA TCTCTCCTGT CTACTTCGTC ACCTTCGTCC TCATCGCACA GTTCGTCCTG 
GTCAACGTGG TGGTGGCCGT GCTGATGAAG CACTTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGACGCTG AGATGGACGC TGAGATCGAG CTGGAGATGT CCCGAGGAGC GAGCACCACC 
AGTGGCAGCA GGGGAGGTGC GGTGGCCCTG GAGAGCAGAG CATCCTGCAG AAGCCAGGAG 
GCCATGAAGT CAGAGCCAGC AGCGCAGGCG AAGCACCAAG CCCTGGAGGC GCTGAGCTGT 
GAGAATGTGT CTGTAGCCAT CCCAGACACG CTGGAGGGGG CTGAGACTCC CATGATGGAA 
ATCCTGTCCT TCTGCTCTGT TTCCCACCAT GTCCTGACAC CTTCCCACCA CCCCCGAGGC 
CGCGAGGCTG AGGGAGGCAC GGCCATCCCG CAGCCTCAGG ACTCCCAGAA CATGCTGACG 
GTCCGCAAGA TCTCCGTTTC ACGCATGCAC TCCTTGCCCA ACGACAGCTA CATGTTCCGG 
CCCGTGATGC CAGCCAAAGC TCCCTTTCCC CTCCACGAGG TGGAGATGGA GGCATATCCC 
TGCAAGTCAC AGCGAGGGTC CATCACCTCC ATCCGCTCGC AGCCCGTGGG GACGTGCTCC 
TCCTTGAGGG TCCCCCCCGA GTCCCTCCAC GTGGCCGTGC GCTCCCCCGG CCGTGAGGGC 
TGGCACCGCT GGAAGGTCCT TCCCCCGCAC GTCGTCCCTC GGTCTCCTAC TCTCAGCAAG 
CTGCTGTGCC GGCAGGAGGC CGTTCGGACA GACTCGGTGG ACGGACACCC TCCAGACCCC 
AAGGACAACC TGCAAGCAGA GCGTGGAGAG ATGCCACCAT CACCAAAGCA CCCTCAGCGC 
ACCCTCACTC CCAGCCCTCT CCACCCTGCA GCCTCCTCCC TGCCGGGCTC CCCTCCGCGC 
TCCCCACCAA CCTCCCCACA CCGGCACCCA TCGCTGACTC GGAAACACCC CTGCAGCCAG 
CGCGGGGTCC CCAGTCCCAC CGAGAGCCCC CCAAGCCCCC AGGGCTGCCT CCTGGAGCCC 
TCTGACCTGG CTGACGAGGA GGTTCGCCAC ATCAACAGCT CAGCCAAAGA CTGGGGGGAG 
CACTCGGAGG CCGGGAGCTG CTCCACGTCG CCGTTCGTCT CGCCGGCCGC GTCGCCGGTG 
CCCCTGAAGA ATTGCAGCAC AAGCAGCCTG ACGGCAGGCA GCGGCAAGGA GAGGGACTTG 
AAGAAGTTTT ACAGCATCGA TACGAAGGGC TTCCTCACCA AGCCCAGCTG GGCGGATGAC 
CAAAGGCGGC ACTCCATCGA GATCTGCCCC TCCGTCCGCG ATGGGGACTG CGGCTTCGAG 
GAGCGCGCCG AGGAAAAGCC ACGCTCCCCC GCCCACGTCC AGCCAGAGTC CGAGTACATC 
CACGGAGCCC GGAGGAAGAA GAAGATGAGC CCGCCCTGCA TTTCTATAGA TCCTCCCATG 
GAGGACGACA GCGGGGCGGC CTCGCGCACC AAACCCTCCG AGAACAGCAT GCTGCGCCGC 
AGGACCCCGT CCTGCGAGTT CCCGGCGTAC CGAGAGCCCC TGGAGCCGGC GGAGGCGCAG 
CCGGGGGAGC CGGGCTGCAA AGCAGAGCGC CGTGCCCAGC ACCTCGCCAT CCCCGGCTTC 
TCCTTCGAGC AGTCGGATGG CGGCGGCTCC CTGAGCAGCC TCTCAGACCT GCTGGACAGT 
GGGCGATCCA CACCGGACTG CGAACTGAGA AAGCCGGAGC CCTTAAAAAC TCAACGGAGC 
AACGCCGAGA AAAGCAAAGA GCTGCTCAGC GTGGCCAAGA GCCCCGTCCG GATGCAGGAC 
TTGGTGACTG TTGCAACAGA AGAAGACATC GACGAGCCCG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1H ( XM_015294424.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149910.2 Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X3, mRNA.

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CloneID	OGa144533
Clone ID Related Accession (Same CDS sequence)	XM_015294424.2
Accession Version	XM_015294424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTTTCTGAAA CAAAGCAGCG GGAGAACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTGAGCAT GGGCATCGAG TACCACGAAC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTGCTCCG GGTGCTGAAG TTGGTGCGCT TCATGCCCGC CCTCCGTCGC 
CAGCTGGTGG TCCTGATGAA GACAATGGAC AACGTTGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTGGTGGTG GAGAACTTTC ACAAGTGCCG GCAGCACCAG 
GAGGCAGAGG AGGCCCGGCG TCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGGAG GCACGGCCAT CCCGCAGCCT CAGGACTCCC AGAACATGCT GACGGTCCGC 
AAGATCTCCG TTTCACGCAT GCACTCCTTG CCCAACGACA GCTACATGTT CCGGCCCGTG 
ATGCCAGCCA AAGCTCCCTT TCCCCTCCAC GAGGTGGAGA TGGAGGCATA TCCCTGCAAG 
TCACAGCGAG GGTCCATCAC CTCCATCCGC TCGCAGCCCG TGGGGACGTG CTCCTCCTTG 
AGGGTCCCCC CCGAGTCCCT CCACGTGGCC GTGCGCTCCC CCGGCCGTGA GGGCTGGCAC 
CGCTGGAAGG TCCTTCCCCC GCACGTCGTC CCTCGGTCTC CTACTCTCAG CAAGCTGCTG 
TGCCGGCAGG AGGCCGTTCG GACAGACTCG GTGGACGGAC ACCCTCCAGA CCCCAAGGAC 
AACCTGCAAG CAGAGCGTGG AGAGATGCCA CCATCACCAA AGCACCCTCA GCGCACCCTC 
ACTCCCAGCC CTCTCCACCC TGCAGCCTCC TCCCTGCCGG GCTCCCCTCC GCGCTCCCCA 
CCAACCTCCC CACACCGGCA CCCATCGCTG ACTCGGAAAC ACCCCTGCAG CCAGCGCGGG 
GTCCCCAGTC CCACCGAGAG CCCCCCAAGC CCCCAGGGCT GCCTCCTGGA GCCCTCTGAC 
CTGGCTGACG AGGAGGTTCG CCACATCAAC AGCTCAGCCA AAGACTGGGG GGAGCACTCG 
GAGGCCGGGA GCTGCTCCAC GTCGCCGTTC GTCTCGCCGG CCGCGTCGCC GGTGCCCCTG 
AAGAATTGCA GCACAAGCAG CCTGACGGCA GGCAGCGGCA AGGAGAGGGA CTTGAAGAAG 
TTTTACAGCA TCGATACGAA GGGCTTCCTC ACCAAGCCCA GCTGGGCGGA TGACCAAAGG 
CGGCACTCCA TCGAGATCTG CCCCTCCGTC CGCGATGGGG ACTGCGGCTT CGAGGAGCGC 
GCCGAGGAAA AGCCACGCTC CCCCGCCCAC GTCCAGCCAG AGTCCGAGTA CATCCACGGA 
GCCCGGAGGA AGAAGAAGAT GAGCCCGCCC TGCATTTCTA TAGATCCTCC CATGGAGGAC 
GACAGCGGGG CGGCCTCGCG CACCAAACCC TCCGAGAACA GCATGCTGCG CCGCAGGACC 
CCGTCCTGCG AGTTCCCGGC GTACCGAGAG CCCCTGGAGC CGGCGGAGGC GCAGCCGGGG 
GAGCCGGGCT GCAAAGCAGA GCGCCGTGCC CAGCACCTCG CCATCCCCGG CTTCTCCTTC 
GAGCAGTCGG ATGGCGGCGG CTCCCTGAGC AGCCTCTCAG ACCTGCTGGA CAGTGGGCGA 
TCCACACCGG ACTGCGAACT GAGAAAGCCG GAGCCCTTAA AAACTCAACG GAGCAACGCC 
GAGAAAAGCA AAGAGCTGCT CAGCGTGGCC AAGAGCCCCG TCCGGATGCA GGACTTGGTG 
ACTGTTGCAA CAGAAGAAGA CATCGACGAG CCCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149910.2
CDS	374..7612
Translation	MSDGESREPRPAGGEVRVPITVPPRRAAEQPLDDAGSAPSGSPSRDPPPDLASEEEEQVPYPALAPTAFFCLKQTTRPRSWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDVECQSERCTILEAFDDFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDRNLATTYNLTFLHPYYRTDEAEENPFICSSHRENGMQKCSNIPTRKEYKVECTLSMDSYTPSLYNPDFSSKNACINWNQYYNVCMAGDVNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTIRLYNNWQSKRRKKVNPNNTTNGQSRRGKKRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSNPEICDLELKVMKPGGQLMLPSPSPNLQSPVPPPDSESVHSIYHADCHVEGPQVNCKSSNAPASIKLTTGLSNHGNMNYPTILPSPTSKASSVPVPKGKKNGSSPVAVVNSPVTLGTDSYGKLQQLVGEHGLRRTPSRLSGLSVACPLPSPPGGTLSCELQDCPFCASLLEDPEFAFSESDSCDSDSNGIYEFTQDLRHGDHRDQLQQQRGRRKRKKKKPKERNKVTRLWKAFGSKLKRIVESKYFNRGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSMFALEMLLKLLAFGLFGYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSANFDDDFEKLKDLRATEMKMYSLAVTPNGHLEGRGSMPPPIIMRTAATPMPTPKCSPHMDSVHTFVDSRRGSNASLDPLTYDQKSSSSLRSSPCANWGTNSNWGSRRSSWNSLGRAPSLKKKNQSGERESLLSGEGKGSTDDDSDDAKSSTVSRPSLHRRAESLDYRSSLDLPELLQLPPMRHSLSISPMAVLPAEYQDCNGKMVHVPSEFFLHIDGHKEEAVDYEDDMEDSYCYRIRKVLEPYKPEWCKSHEDWSLYLFSPQNRFRVMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPHSTERIFLSVSNYIFTAIFVAEMMVKVVALGFFSGENTYLQSSWNVLDGVLVFVSIIDIIVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCDGPDVKNITTKTDCTNAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPIQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKEMYLSEAQRRPYYADYSPARKYIHTLCTSHYLDLFITFIIGVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSIVGITLEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCSEENPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIELEMSRGASTTSGSRGGAVALESRASCRSQEAMKSEPAAQAKHQALGGTAIPQPQDSQNMLTVRKISVSRMHSLPNDSYMFRPVMPAKAPFPLHEVEMEAYPCKSQRGSITSIRSQPVGTCSSLRVPPESLHVAVRSPGREGWHRWKVLPPHVVPRSPTLSKLLCRQEAVRTDSVDGHPPDPKDNLQAERGEMPPSPKHPQRTLTPSPLHPAASSLPGSPPRSPPTSPHRHPSLTRKHPCSQRGVPSPTESPPSPQGCLLEPSDLADEEVRHINSSAKDWGEHSEAGSCSTSPFVSPAASPVPLKNCSTSSLTAGSGKERDLKKFYSIDTKGFLTKPSWADDQRRHSIEICPSVRDGDCGFEERAEEKPRSPAHVQPESEYIHGARRKKKMSPPCISIDPPMEDDSGAASRTKPSENSMLRRRTPSCEFPAYREPLEPAEAQPGEPGCKAERRAQHLAIPGFSFEQSDGGGSLSSLSDLLDSGRSTPDCELRKPEPLKTQRSNAEKSKELLSVAKSPVRMQDLVTVATEEDIDEPV

Target ORF information:

RefSeq Version	XM_015294424.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294424.2

ORF Insert Sequence:

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTTTCTGAAA CAAAGCAGCG GGAGAACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTGAGCAT GGGCATCGAG TACCACGAAC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTGCTCCG GGTGCTGAAG TTGGTGCGCT TCATGCCCGC CCTCCGTCGC 
CAGCTGGTGG TCCTGATGAA GACAATGGAC AACGTTGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTGGTGGTG GAGAACTTTC ACAAGTGCCG GCAGCACCAG 
GAGGCAGAGG AGGCCCGGCG TCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGGAG GCACGGCCAT CCCGCAGCCT CAGGACTCCC AGAACATGCT GACGGTCCGC 
AAGATCTCCG TTTCACGCAT GCACTCCTTG CCCAACGACA GCTACATGTT CCGGCCCGTG 
ATGCCAGCCA AAGCTCCCTT TCCCCTCCAC GAGGTGGAGA TGGAGGCATA TCCCTGCAAG 
TCACAGCGAG GGTCCATCAC CTCCATCCGC TCGCAGCCCG TGGGGACGTG CTCCTCCTTG 
AGGGTCCCCC CCGAGTCCCT CCACGTGGCC GTGCGCTCCC CCGGCCGTGA GGGCTGGCAC 
CGCTGGAAGG TCCTTCCCCC GCACGTCGTC CCTCGGTCTC CTACTCTCAG CAAGCTGCTG 
TGCCGGCAGG AGGCCGTTCG GACAGACTCG GTGGACGGAC ACCCTCCAGA CCCCAAGGAC 
AACCTGCAAG CAGAGCGTGG AGAGATGCCA CCATCACCAA AGCACCCTCA GCGCACCCTC 
ACTCCCAGCC CTCTCCACCC TGCAGCCTCC TCCCTGCCGG GCTCCCCTCC GCGCTCCCCA 
CCAACCTCCC CACACCGGCA CCCATCGCTG ACTCGGAAAC ACCCCTGCAG CCAGCGCGGG 
GTCCCCAGTC CCACCGAGAG CCCCCCAAGC CCCCAGGGCT GCCTCCTGGA GCCCTCTGAC 
CTGGCTGACG AGGAGGTTCG CCACATCAAC AGCTCAGCCA AAGACTGGGG GGAGCACTCG 
GAGGCCGGGA GCTGCTCCAC GTCGCCGTTC GTCTCGCCGG CCGCGTCGCC GGTGCCCCTG 
AAGAATTGCA GCACAAGCAG CCTGACGGCA GGCAGCGGCA AGGAGAGGGA CTTGAAGAAG 
TTTTACAGCA TCGATACGAA GGGCTTCCTC ACCAAGCCCA GCTGGGCGGA TGACCAAAGG 
CGGCACTCCA TCGAGATCTG CCCCTCCGTC CGCGATGGGG ACTGCGGCTT CGAGGAGCGC 
GCCGAGGAAA AGCCACGCTC CCCCGCCCAC GTCCAGCCAG AGTCCGAGTA CATCCACGGA 
GCCCGGAGGA AGAAGAAGAT GAGCCCGCCC TGCATTTCTA TAGATCCTCC CATGGAGGAC 
GACAGCGGGG CGGCCTCGCG CACCAAACCC TCCGAGAACA GCATGCTGCG CCGCAGGACC 
CCGTCCTGCG AGTTCCCGGC GTACCGAGAG CCCCTGGAGC CGGCGGAGGC GCAGCCGGGG 
GAGCCGGGCT GCAAAGCAGA GCGCCGTGCC CAGCACCTCG CCATCCCCGG CTTCTCCTTC 
GAGCAGTCGG ATGGCGGCGG CTCCCTGAGC AGCCTCTCAG ACCTGCTGGA CAGTGGGCGA 
TCCACACCGG ACTGCGAACT GAGAAAGCCG GAGCCCTTAA AAACTCAACG GAGCAACGCC 
GAGAAAAGCA AAGAGCTGCT CAGCGTGGCC AAGAGCCCCG TCCGGATGCA GGACTTGGTG 
ACTGTTGCAA CAGAAGAAGA CATCGACGAG CCCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1H ( XM_015294422.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149908.1 Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X1, mRNA.

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CloneID	OGa42352
Clone ID Related Accession (Same CDS sequence)	XM_015294422.1
Accession Version	XM_015294422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTCTCAGAAA CCAAGCAGCG GGAGCACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTCAGCAT GGGCATCGAG TACCACGAGC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTCCTGCG GGTGCTGAAG TTGGTGCGCT TCATGCCGGC CCTGCGCCGC 
CAGCTTGTGG TCCTGATGAA GACTATGGAC AACGTAGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG 
GAGGCTGAGG AGGCCCGGCG GCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGAGG CGCTGAGCTG TGAGAATGTG TCTGTAGCCA TCCCAGACAC GCTGGAGGGG 
GCTGAGACTC CCATGATGGA AATCCTGTCC TTCTGCTCTG TTTCCCACCA TGTCCTGACA 
CCTTCCCACC ACCCCCGAGG CCGCGAGGCT GAGGGAGGCA CGGCCATCCC GCAGCCTCAG 
GACTCCCAGA ACATGCTGAC GGTCCGCAAG ATCTCCGTTT CACGCATGCA CTCCTTGCCC 
AACGACAGCT ACATGTTCCG GCCCGTGATG CCAGCCAAAG CTCCCTTTCC CCTCCACGAG 
GTGGAGATGG AGGCATATCC CTGCAAGTCA CAGCGAGGGT CCATCACCTC CATCCGCTCG 
CAGCCCGTGG GGACGTGCTC CTCCTTGAGG GTCCCCCCCG AGTCCCTCCA CGTGGCCGTG 
CGCTCCCCCG GCCGTGAGGG CTGGCACCGC TGGAAGGTCC TTCCCCCGCA CGTCGTCCCT 
CGGTCTCCTA CTCTCAGCAA GCTGCTGTGC CGGCAGGAGG CCGTTCGGAC AGACTCGGTG 
GACGGACACC CTCCAGACCC CAAGGACAAC CTGCAAGCAG AGCGTGGAGA GATGCCACCA 
TCACCAAAGC ACCCTCAGCG CACCCTCACT CCCAGCCCTC TCCACCCTGC AGCCTCCTCC 
CTGCCGGGCT CCCCTCCGCG CTCCCCACCA ACCTCCCCAC ACCGGCACCC ATCGCTGACT 
CGGAAACACC CCTGCAGCCA GCGCGGGGTC CCCAGTCCCA CCGAGAGCCC CCCAAGCCCC 
CAGGGCTGCC TCCTGGAGCC CTCTGACCTG GCTGACGAGG AGGTTCGCCA CATCAACAGC 
TCAGCCAAAG ACTGGGGGGA GCACTCGGAG GCCGGGAGCT GCTCCACGTC GCCGTTCGTC 
TCGCCGGCCG CGTCGCCGGT GCCCCTGAAG AATTGCAGCA CAAGCAGCCT GACGGCAGGC 
AGCGGCAAGG AGAGGGACTT GAAGAAGTTT TACAGCATCG ATACGAAGGG CTTCCTCACC 
AAGCCCAGCT GGGCGGATGA CCAAAGGCGG CACTCCATCG AGATCTGCCC CTCCGTCCGC 
GATGGGGACT GCGGCTTCGA GGAGCGCGCC GAGGAAAAGC CACGCTCCCC CGCCCACGTC 
CAGCCAGAGT CCGAGTACAT CCACGGAGCC CGGAGGAAGA AGAAGATGAG CCCGCCCTGC 
ATTTCTATAG ATCCTCCCAT GGAGGACGAC AGCGGGGCGG CCTCGCGCAC CAAACCCTCC 
GAGAACAGCA TGCTGCGCCG CAGGACCCCG TCCTGCGAGT TCCCGGCGTA CCGAGAGCCC 
CTGGAGCCGG CGGAGGCGCA GCCGGGGGAG CCGGGCTGCA AAGCAGAGCG CCGTGCCCAG 
CACCTCGCCA TCCCCGGCTT CTCCTTCGAG CAGTCGGATG GCGGCGGCTC CCTGAGCAGC 
CTCTCAGACC TGCTGGACAG TGGGCGATCC ACACCGGACT GCGAACTGAG AAAGCCGGAG 
CCCTTAAAAA CTCAACGGAG CAACGCCGAG AAAAGCAAAG AGCTGCTCAG CGTGGCCAAG 
AGCCCCGTCC GGATGCAGGA CTTGGTGACT GTTGCAACAG AAGAAGACAT CGACGAGCCC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149908.1
CDS	383..7768
Translation	MSDGESREPRPAGGEVRVPITVPPRRAAEQPLDDAGSAPSGSPSRDPPPDLASEEEEQVPYPALAPTAFFCLKQTTRPRSWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDVECQSERCTILEAFDDFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDRNLATTYNLTFLHPYYRTDEAEENPFICSSHRENGMQKCSNIPTRKEYKVECTLSMDSYTPSLYNPDFSSKNACINWNQYYNVCMAGDVNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTIRLYNNWQSKRRKKVNPNNTTNGQSRRGKKRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSNPEICDLELKVMKPGGQLMLPSPSPNLQSPVPPPDSESVHSIYHADCHVEGPQVNCKSSNAPASIKLTTGLSNHGNMNYPTILPSPTSKASSVPVPKGKKNGSSPVAVVNSPVTLGTDSYGKLQQLVGEHGLRRTPSRLSGLSVACPLPSPPGGTLSCELQDCPFCASLLEDPEFAFSESDSCDSDSNGIYEFTQDLRHGDHRDQLQQQRGRRKRKKKKPKERNKVTRLWKAFGSKLKRIVESKYFNRGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSMFALEMLLKLLAFGLFGYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSANFDDDFEKLKDLRATEMKMYSLAVTPNGHLEGRGSMPPPIIMRTAATPMPTPKCSPHMDSVHTFVDSRRGSNASLDPLTYDQKSSSSLRSSPCANWGTNSNWGSRRSSWNSLGRAPSLKKKNQSGERESLLSGEGKGSTDDDSDDAKSSTVSRPSLHRRAESLDYRSSLDLPELLQLPPMRHSLSISPMAVLPAEYQDCNGKMVHVPSEFFLHIDGHKEEAVDYEDDMEDSYCYRIRKVLEPYKPEWCKSHEDWSLYLFSPQNRFRVMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPHSTERIFLSVSNYIFTAIFVAEMMVKVVALGFFSGENTYLQSSWNVLDGVLVFVSIIDIIVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCDGPDVKNITTKTDCTNAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPIQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKEMYLSEAQRRPYYADYSPARKYIHTLCTSHYLDLFITFIIGVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSIVGITLEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCSEENPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIELEMSRGASTTSGSRGGAVALESRASCRSQEAMKSEPAAQAKHQALEALSCENVSVAIPDTLEGAETPMMEILSFCSVSHHVLTPSHHPRGREAEGGTAIPQPQDSQNMLTVRKISVSRMHSLPNDSYMFRPVMPAKAPFPLHEVEMEAYPCKSQRGSITSIRSQPVGTCSSLRVPPESLHVAVRSPGREGWHRWKVLPPHVVPRSPTLSKLLCRQEAVRTDSVDGHPPDPKDNLQAERGEMPPSPKHPQRTLTPSPLHPAASSLPGSPPRSPPTSPHRHPSLTRKHPCSQRGVPSPTESPPSPQGCLLEPSDLADEEVRHINSSAKDWGEHSEAGSCSTSPFVSPAASPVPLKNCSTSSLTAGSGKERDLKKFYSIDTKGFLTKPSWADDQRRHSIEICPSVRDGDCGFEERAEEKPRSPAHVQPESEYIHGARRKKKMSPPCISIDPPMEDDSGAASRTKPSENSMLRRRTPSCEFPAYREPLEPAEAQPGEPGCKAERRAQHLAIPGFSFEQSDGGGSLSSLSDLLDSGRSTPDCELRKPEPLKTQRSNAEKSKELLSVAKSPVRMQDLVTVATEEDIDEPV

Target ORF information:

RefSeq Version	XM_015294422.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294422.1

ORF Insert Sequence:

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTCTCAGAAA CCAAGCAGCG GGAGCACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTCAGCAT GGGCATCGAG TACCACGAGC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTCCTGCG GGTGCTGAAG TTGGTGCGCT TCATGCCGGC CCTGCGCCGC 
CAGCTTGTGG TCCTGATGAA GACTATGGAC AACGTAGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG 
GAGGCTGAGG AGGCCCGGCG GCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGAGG CGCTGAGCTG TGAGAATGTG TCTGTAGCCA TCCCAGACAC GCTGGAGGGG 
GCTGAGACTC CCATGATGGA AATCCTGTCC TTCTGCTCTG TTTCCCACCA TGTCCTGACA 
CCTTCCCACC ACCCCCGAGG CCGCGAGGCT GAGGGAGGCA CGGCCATCCC GCAGCCTCAG 
GACTCCCAGA ACATGCTGAC GGTCCGCAAG ATCTCCGTTT CACGCATGCA CTCCTTGCCC 
AACGACAGCT ACATGTTCCG GCCCGTGATG CCAGCCAAAG CTCCCTTTCC CCTCCACGAG 
GTGGAGATGG AGGCATATCC CTGCAAGTCA CAGCGAGGGT CCATCACCTC CATCCGCTCG 
CAGCCCGTGG GGACGTGCTC CTCCTTGAGG GTCCCCCCCG AGTCCCTCCA CGTGGCCGTG 
CGCTCCCCCG GCCGTGAGGG CTGGCACCGC TGGAAGGTCC TTCCCCCGCA CGTCGTCCCT 
CGGTCTCCTA CTCTCAGCAA GCTGCTGTGC CGGCAGGAGG CCGTTCGGAC AGACTCGGTG 
GACGGACACC CTCCAGACCC CAAGGACAAC CTGCAAGCAG AGCGTGGAGA GATGCCACCA 
TCACCAAAGC ACCCTCAGCG CACCCTCACT CCCAGCCCTC TCCACCCTGC AGCCTCCTCC 
CTGCCGGGCT CCCCTCCGCG CTCCCCACCA ACCTCCCCAC ACCGGCACCC ATCGCTGACT 
CGGAAACACC CCTGCAGCCA GCGCGGGGTC CCCAGTCCCA CCGAGAGCCC CCCAAGCCCC 
CAGGGCTGCC TCCTGGAGCC CTCTGACCTG GCTGACGAGG AGGTTCGCCA CATCAACAGC 
TCAGCCAAAG ACTGGGGGGA GCACTCGGAG GCCGGGAGCT GCTCCACGTC GCCGTTCGTC 
TCGCCGGCCG CGTCGCCGGT GCCCCTGAAG AATTGCAGCA CAAGCAGCCT GACGGCAGGC 
AGCGGCAAGG AGAGGGACTT GAAGAAGTTT TACAGCATCG ATACGAAGGG CTTCCTCACC 
AAGCCCAGCT GGGCGGATGA CCAAAGGCGG CACTCCATCG AGATCTGCCC CTCCGTCCGC 
GATGGGGACT GCGGCTTCGA GGAGCGCGCC GAGGAAAAGC CACGCTCCCC CGCCCACGTC 
CAGCCAGAGT CCGAGTACAT CCACGGAGCC CGGAGGAAGA AGAAGATGAG CCCGCCCTGC 
ATTTCTATAG ATCCTCCCAT GGAGGACGAC AGCGGGGCGG CCTCGCGCAC CAAACCCTCC 
GAGAACAGCA TGCTGCGCCG CAGGACCCCG TCCTGCGAGT TCCCGGCGTA CCGAGAGCCC 
CTGGAGCCGG CGGAGGCGCA GCCGGGGGAG CCGGGCTGCA AAGCAGAGCG CCGTGCCCAG 
CACCTCGCCA TCCCCGGCTT CTCCTTCGAG CAGTCGGATG GCGGCGGCTC CCTGAGCAGC 
CTCTCAGACC TGCTGGACAG TGGGCGATCC ACACCGGACT GCGAACTGAG AAAGCCGGAG 
CCCTTAAAAA CTCAACGGAG CAACGCCGAG AAAAGCAAAG AGCTGCTCAG CGTGGCCAAG 
AGCCCCGTCC GGATGCAGGA CTTGGTGACT GTTGCAACAG AAGAAGACAT CGACGAGCCC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1H ( XM_015294423.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149909.1 Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X2, mRNA.

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CloneID	OGa42353
Clone ID Related Accession (Same CDS sequence)	XM_015294423.1
Accession Version	XM_015294423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTCTCAGAAA CCAAGCAGCG GGAGCACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTCAGCAT GGGCATCGAG TACCACGAGC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTCCTGCG GGTGCTGAAG TTGGTGCGCT TCATGCCGGC CCTGCGCCGC 
CAGCTTGTGG TCCTGATGAA GACTATGGAC AACGTAGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG 
GAGGCTGAGG AGGCCCGGCG GCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAAAGCCC AGCGCAGGCC TTACTATGCT GACTATTCTC CAGCACGGAA ATACATTCAC 
ACCCTCTGCA CCAGCCACTA TCTCGACCTC TTCATCACCT TCATCATTGG AGTCAACGTC 
ATCACTATGT CGATGGAGCA CTACAACCAG CCAAAGTCCC TGGATGAAGC CCTGAAGTAC 
TGTAACTACG TGTTCACCAT AGTTTTTGTG TTCGAGGCAG TCCTGAAGTT GGTGGCATTT 
GGTTTTCGAA GGTTCTTTAA GGACAGGTGG AATCAGCTGG ATTTGGCCAT CGTTCTCTTA 
TCCATTGTGG GAATCACGCT GGAGGAAATT GAGATGAACG CTGCACTGCC CATCAATCCC 
ACAATAATAC GCATCATGCG GGTGCTGAGA ATAGCAAGAG TGCTGAAACT GCTTAAAATG 
GCCACCGGAA TGCGAGCCCT CCTGGACACA GTGGTACAAG CGCTTCCCCA GGTAGGGAAC 
CTTGGCCTAC TTTTTATGCT CCTGTTTTTT ATCTATGCTG CCCTGGGAGT GGAATTGTTT 
GGCAAGCTAG ACTGCAGCGA AGAGAATCCT TGCGAAGGGC TGAGCCGGCA CGCCACGTTC 
ACCAACTTTG GGATGGCCTT CCTCACCCTC TTCAGGGTGT CCACGGGGGA CAACTGGAAC 
GGTATCATGA AGGACACTCT CCGGGAATGC ACGCGGGAAG ACAAGCACTG CCTCAGCTAC 
CTGCCTGTTA TCTCTCCTGT CTACTTCGTC ACCTTCGTCC TCATCGCACA GTTCGTCCTG 
GTCAACGTGG TGGTGGCCGT GCTGATGAAG CACTTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGACGCTG AGATGGACGC TGAGATCGAG CTGGAGATGT CCCGAGGAGC GAGCACCACC 
AGTGGCAGCA GGGGAGGTGC GGTGGCCCTG GAGAGCAGAG CATCCTGCAG AAGCCAGGAG 
GCCATGAAGT CAGAGCCAGC AGCGCAGGCG AAGCACCAAG CCCTGGAGGC GCTGAGCTGT 
GAGAATGTGT CTGTAGCCAT CCCAGACACG CTGGAGGGGG CTGAGACTCC CATGATGGAA 
ATCCTGTCCT TCTGCTCTGT TTCCCACCAT GTCCTGACAC CTTCCCACCA CCCCCGAGGC 
CGCGAGGCTG AGGGAGGCAC GGCCATCCCG CAGCCTCAGG ACTCCCAGAA CATGCTGACG 
GTCCGCAAGA TCTCCGTTTC ACGCATGCAC TCCTTGCCCA ACGACAGCTA CATGTTCCGG 
CCCGTGATGC CAGCCAAAGC TCCCTTTCCC CTCCACGAGG TGGAGATGGA GGCATATCCC 
TGCAAGTCAC AGCGAGGGTC CATCACCTCC ATCCGCTCGC AGCCCGTGGG GACGTGCTCC 
TCCTTGAGGG TCCCCCCCGA GTCCCTCCAC GTGGCCGTGC GCTCCCCCGG CCGTGAGGGC 
TGGCACCGCT GGAAGGTCCT TCCCCCGCAC GTCGTCCCTC GGTCTCCTAC TCTCAGCAAG 
CTGCTGTGCC GGCAGGAGGC CGTTCGGACA GACTCGGTGG ACGGACACCC TCCAGACCCC 
AAGGACAACC TGCAAGCAGA GCGTGGAGAG ATGCCACCAT CACCAAAGCA CCCTCAGCGC 
ACCCTCACTC CCAGCCCTCT CCACCCTGCA GCCTCCTCCC TGCCGGGCTC CCCTCCGCGC 
TCCCCACCAA CCTCCCCACA CCGGCACCCA TCGCTGACTC GGAAACACCC CTGCAGCCAG 
CGCGGGGTCC CCAGTCCCAC CGAGAGCCCC CCAAGCCCCC AGGGCTGCCT CCTGGAGCCC 
TCTGACCTGG CTGACGAGGA GGTTCGCCAC ATCAACAGCT CAGCCAAAGA CTGGGGGGAG 
CACTCGGAGG CCGGGAGCTG CTCCACGTCG CCGTTCGTCT CGCCGGCCGC GTCGCCGGTG 
CCCCTGAAGA ATTGCAGCAC AAGCAGCCTG ACGGCAGGCA GCGGCAAGGA GAGGGACTTG 
AAGAAGTTTT ACAGCATCGA TACGAAGGGC TTCCTCACCA AGCCCAGCTG GGCGGATGAC 
CAAAGGCGGC ACTCCATCGA GATCTGCCCC TCCGTCCGCG ATGGGGACTG CGGCTTCGAG 
GAGCGCGCCG AGGAAAAGCC ACGCTCCCCC GCCCACGTCC AGCCAGAGTC CGAGTACATC 
CACGGAGCCC GGAGGAAGAA GAAGATGAGC CCGCCCTGCA TTTCTATAGA TCCTCCCATG 
GAGGACGACA GCGGGGCGGC CTCGCGCACC AAACCCTCCG AGAACAGCAT GCTGCGCCGC 
AGGACCCCGT CCTGCGAGTT CCCGGCGTAC CGAGAGCCCC TGGAGCCGGC GGAGGCGCAG 
CCGGGGGAGC CGGGCTGCAA AGCAGAGCGC CGTGCCCAGC ACCTCGCCAT CCCCGGCTTC 
TCCTTCGAGC AGTCGGATGG CGGCGGCTCC CTGAGCAGCC TCTCAGACCT GCTGGACAGT 
GGGCGATCCA CACCGGACTG CGAACTGAGA AAGCCGGAGC CCTTAAAAAC TCAACGGAGC 
AACGCCGAGA AAAGCAAAGA GCTGCTCAGC GTGGCCAAGA GCCCCGTCCG GATGCAGGAC 
TTGGTGACTG TTGCAACAGA AGAAGACATC GACGAGCCCG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149909.1
CDS	383..7747
Translation	MSDGESREPRPAGGEVRVPITVPPRRAAEQPLDDAGSAPSGSPSRDPPPDLASEEEEQVPYPALAPTAFFCLKQTTRPRSWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDVECQSERCTILEAFDDFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDRNLATTYNLTFLHPYYRTDEAEENPFICSSHRENGMQKCSNIPTRKEYKVECTLSMDSYTPSLYNPDFSSKNACINWNQYYNVCMAGDVNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTIRLYNNWQSKRRKKVNPNNTTNGQSRRGKKRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSNPEICDLELKVMKPGGQLMLPSPSPNLQSPVPPPDSESVHSIYHADCHVEGPQVNCKSSNAPASIKLTTGLSNHGNMNYPTILPSPTSKASSVPVPKGKKNGSSPVAVVNSPVTLGTDSYGKLQQLVGEHGLRRTPSRLSGLSVACPLPSPPGGTLSCELQDCPFCASLLEDPEFAFSESDSCDSDSNGIYEFTQDLRHGDHRDQLQQQRGRRKRKKKKPKERNKVTRLWKAFGSKLKRIVESKYFNRGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSMFALEMLLKLLAFGLFGYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSANFDDDFEKLKDLRATEMKMYSLAVTPNGHLEGRGSMPPPIIMRTAATPMPTPKCSPHMDSVHTFVDSRRGSNASLDPLTYDQKSSSSLRSSPCANWGTNSNWGSRRSSWNSLGRAPSLKKKNQSGERESLLSGEGKGSTDDDSDDAKSSTVSRPSLHRRAESLDYRSSLDLPELLQLPPMRHSLSISPMAVLPAEYQDCNGKMVHVPSEFFLHIDGHKEEAVDYEDDMEDSYCYRIRKVLEPYKPEWCKSHEDWSLYLFSPQNRFRVMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPHSTERIFLSVSNYIFTAIFVAEMMVKVVALGFFSGENTYLQSSWNVLDGVLVFVSIIDIIVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCDGPDVKNITTKTDCTNAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPIQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRRPYYADYSPARKYIHTLCTSHYLDLFITFIIGVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSIVGITLEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCSEENPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIELEMSRGASTTSGSRGGAVALESRASCRSQEAMKSEPAAQAKHQALEALSCENVSVAIPDTLEGAETPMMEILSFCSVSHHVLTPSHHPRGREAEGGTAIPQPQDSQNMLTVRKISVSRMHSLPNDSYMFRPVMPAKAPFPLHEVEMEAYPCKSQRGSITSIRSQPVGTCSSLRVPPESLHVAVRSPGREGWHRWKVLPPHVVPRSPTLSKLLCRQEAVRTDSVDGHPPDPKDNLQAERGEMPPSPKHPQRTLTPSPLHPAASSLPGSPPRSPPTSPHRHPSLTRKHPCSQRGVPSPTESPPSPQGCLLEPSDLADEEVRHINSSAKDWGEHSEAGSCSTSPFVSPAASPVPLKNCSTSSLTAGSGKERDLKKFYSIDTKGFLTKPSWADDQRRHSIEICPSVRDGDCGFEERAEEKPRSPAHVQPESEYIHGARRKKKMSPPCISIDPPMEDDSGAASRTKPSENSMLRRRTPSCEFPAYREPLEPAEAQPGEPGCKAERRAQHLAIPGFSFEQSDGGGSLSSLSDLLDSGRSTPDCELRKPEPLKTQRSNAEKSKELLSVAKSPVRMQDLVTVATEEDIDEPV

Target ORF information:

RefSeq Version	XM_015294423.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294423.1

ORF Insert Sequence:

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTCTCAGAAA CCAAGCAGCG GGAGCACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTCAGCAT GGGCATCGAG TACCACGAGC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTCCTGCG GGTGCTGAAG TTGGTGCGCT TCATGCCGGC CCTGCGCCGC 
CAGCTTGTGG TCCTGATGAA GACTATGGAC AACGTAGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG 
GAGGCTGAGG AGGCCCGGCG GCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAAAGCCC AGCGCAGGCC TTACTATGCT GACTATTCTC CAGCACGGAA ATACATTCAC 
ACCCTCTGCA CCAGCCACTA TCTCGACCTC TTCATCACCT TCATCATTGG AGTCAACGTC 
ATCACTATGT CGATGGAGCA CTACAACCAG CCAAAGTCCC TGGATGAAGC CCTGAAGTAC 
TGTAACTACG TGTTCACCAT AGTTTTTGTG TTCGAGGCAG TCCTGAAGTT GGTGGCATTT 
GGTTTTCGAA GGTTCTTTAA GGACAGGTGG AATCAGCTGG ATTTGGCCAT CGTTCTCTTA 
TCCATTGTGG GAATCACGCT GGAGGAAATT GAGATGAACG CTGCACTGCC CATCAATCCC 
ACAATAATAC GCATCATGCG GGTGCTGAGA ATAGCAAGAG TGCTGAAACT GCTTAAAATG 
GCCACCGGAA TGCGAGCCCT CCTGGACACA GTGGTACAAG CGCTTCCCCA GGTAGGGAAC 
CTTGGCCTAC TTTTTATGCT CCTGTTTTTT ATCTATGCTG CCCTGGGAGT GGAATTGTTT 
GGCAAGCTAG ACTGCAGCGA AGAGAATCCT TGCGAAGGGC TGAGCCGGCA CGCCACGTTC 
ACCAACTTTG GGATGGCCTT CCTCACCCTC TTCAGGGTGT CCACGGGGGA CAACTGGAAC 
GGTATCATGA AGGACACTCT CCGGGAATGC ACGCGGGAAG ACAAGCACTG CCTCAGCTAC 
CTGCCTGTTA TCTCTCCTGT CTACTTCGTC ACCTTCGTCC TCATCGCACA GTTCGTCCTG 
GTCAACGTGG TGGTGGCCGT GCTGATGAAG CACTTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGACGCTG AGATGGACGC TGAGATCGAG CTGGAGATGT CCCGAGGAGC GAGCACCACC 
AGTGGCAGCA GGGGAGGTGC GGTGGCCCTG GAGAGCAGAG CATCCTGCAG AAGCCAGGAG 
GCCATGAAGT CAGAGCCAGC AGCGCAGGCG AAGCACCAAG CCCTGGAGGC GCTGAGCTGT 
GAGAATGTGT CTGTAGCCAT CCCAGACACG CTGGAGGGGG CTGAGACTCC CATGATGGAA 
ATCCTGTCCT TCTGCTCTGT TTCCCACCAT GTCCTGACAC CTTCCCACCA CCCCCGAGGC 
CGCGAGGCTG AGGGAGGCAC GGCCATCCCG CAGCCTCAGG ACTCCCAGAA CATGCTGACG 
GTCCGCAAGA TCTCCGTTTC ACGCATGCAC TCCTTGCCCA ACGACAGCTA CATGTTCCGG 
CCCGTGATGC CAGCCAAAGC TCCCTTTCCC CTCCACGAGG TGGAGATGGA GGCATATCCC 
TGCAAGTCAC AGCGAGGGTC CATCACCTCC ATCCGCTCGC AGCCCGTGGG GACGTGCTCC 
TCCTTGAGGG TCCCCCCCGA GTCCCTCCAC GTGGCCGTGC GCTCCCCCGG CCGTGAGGGC 
TGGCACCGCT GGAAGGTCCT TCCCCCGCAC GTCGTCCCTC GGTCTCCTAC TCTCAGCAAG 
CTGCTGTGCC GGCAGGAGGC CGTTCGGACA GACTCGGTGG ACGGACACCC TCCAGACCCC 
AAGGACAACC TGCAAGCAGA GCGTGGAGAG ATGCCACCAT CACCAAAGCA CCCTCAGCGC 
ACCCTCACTC CCAGCCCTCT CCACCCTGCA GCCTCCTCCC TGCCGGGCTC CCCTCCGCGC 
TCCCCACCAA CCTCCCCACA CCGGCACCCA TCGCTGACTC GGAAACACCC CTGCAGCCAG 
CGCGGGGTCC CCAGTCCCAC CGAGAGCCCC CCAAGCCCCC AGGGCTGCCT CCTGGAGCCC 
TCTGACCTGG CTGACGAGGA GGTTCGCCAC ATCAACAGCT CAGCCAAAGA CTGGGGGGAG 
CACTCGGAGG CCGGGAGCTG CTCCACGTCG CCGTTCGTCT CGCCGGCCGC GTCGCCGGTG 
CCCCTGAAGA ATTGCAGCAC AAGCAGCCTG ACGGCAGGCA GCGGCAAGGA GAGGGACTTG 
AAGAAGTTTT ACAGCATCGA TACGAAGGGC TTCCTCACCA AGCCCAGCTG GGCGGATGAC 
CAAAGGCGGC ACTCCATCGA GATCTGCCCC TCCGTCCGCG ATGGGGACTG CGGCTTCGAG 
GAGCGCGCCG AGGAAAAGCC ACGCTCCCCC GCCCACGTCC AGCCAGAGTC CGAGTACATC 
CACGGAGCCC GGAGGAAGAA GAAGATGAGC CCGCCCTGCA TTTCTATAGA TCCTCCCATG 
GAGGACGACA GCGGGGCGGC CTCGCGCACC AAACCCTCCG AGAACAGCAT GCTGCGCCGC 
AGGACCCCGT CCTGCGAGTT CCCGGCGTAC CGAGAGCCCC TGGAGCCGGC GGAGGCGCAG 
CCGGGGGAGC CGGGCTGCAA AGCAGAGCGC CGTGCCCAGC ACCTCGCCAT CCCCGGCTTC 
TCCTTCGAGC AGTCGGATGG CGGCGGCTCC CTGAGCAGCC TCTCAGACCT GCTGGACAGT 
GGGCGATCCA CACCGGACTG CGAACTGAGA AAGCCGGAGC CCTTAAAAAC TCAACGGAGC 
AACGCCGAGA AAAGCAAAGA GCTGCTCAGC GTGGCCAAGA GCCCCGTCCG GATGCAGGAC 
TTGGTGACTG TTGCAACAGA AGAAGACATC GACGAGCCCG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1H ( XM_015294424.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149910.1 Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X3, mRNA.

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CloneID	OGa42354
Clone ID Related Accession (Same CDS sequence)	XM_015294424.1
Accession Version	XM_015294424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTCTCAGAAA CCAAGCAGCG GGAGCACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTCAGCAT GGGCATCGAG TACCACGAGC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTCCTGCG GGTGCTGAAG TTGGTGCGCT TCATGCCGGC CCTGCGCCGC 
CAGCTTGTGG TCCTGATGAA GACTATGGAC AACGTAGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG 
GAGGCTGAGG AGGCCCGGCG GCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGGAG GCACGGCCAT CCCGCAGCCT CAGGACTCCC AGAACATGCT GACGGTCCGC 
AAGATCTCCG TTTCACGCAT GCACTCCTTG CCCAACGACA GCTACATGTT CCGGCCCGTG 
ATGCCAGCCA AAGCTCCCTT TCCCCTCCAC GAGGTGGAGA TGGAGGCATA TCCCTGCAAG 
TCACAGCGAG GGTCCATCAC CTCCATCCGC TCGCAGCCCG TGGGGACGTG CTCCTCCTTG 
AGGGTCCCCC CCGAGTCCCT CCACGTGGCC GTGCGCTCCC CCGGCCGTGA GGGCTGGCAC 
CGCTGGAAGG TCCTTCCCCC GCACGTCGTC CCTCGGTCTC CTACTCTCAG CAAGCTGCTG 
TGCCGGCAGG AGGCCGTTCG GACAGACTCG GTGGACGGAC ACCCTCCAGA CCCCAAGGAC 
AACCTGCAAG CAGAGCGTGG AGAGATGCCA CCATCACCAA AGCACCCTCA GCGCACCCTC 
ACTCCCAGCC CTCTCCACCC TGCAGCCTCC TCCCTGCCGG GCTCCCCTCC GCGCTCCCCA 
CCAACCTCCC CACACCGGCA CCCATCGCTG ACTCGGAAAC ACCCCTGCAG CCAGCGCGGG 
GTCCCCAGTC CCACCGAGAG CCCCCCAAGC CCCCAGGGCT GCCTCCTGGA GCCCTCTGAC 
CTGGCTGACG AGGAGGTTCG CCACATCAAC AGCTCAGCCA AAGACTGGGG GGAGCACTCG 
GAGGCCGGGA GCTGCTCCAC GTCGCCGTTC GTCTCGCCGG CCGCGTCGCC GGTGCCCCTG 
AAGAATTGCA GCACAAGCAG CCTGACGGCA GGCAGCGGCA AGGAGAGGGA CTTGAAGAAG 
TTTTACAGCA TCGATACGAA GGGCTTCCTC ACCAAGCCCA GCTGGGCGGA TGACCAAAGG 
CGGCACTCCA TCGAGATCTG CCCCTCCGTC CGCGATGGGG ACTGCGGCTT CGAGGAGCGC 
GCCGAGGAAA AGCCACGCTC CCCCGCCCAC GTCCAGCCAG AGTCCGAGTA CATCCACGGA 
GCCCGGAGGA AGAAGAAGAT GAGCCCGCCC TGCATTTCTA TAGATCCTCC CATGGAGGAC 
GACAGCGGGG CGGCCTCGCG CACCAAACCC TCCGAGAACA GCATGCTGCG CCGCAGGACC 
CCGTCCTGCG AGTTCCCGGC GTACCGAGAG CCCCTGGAGC CGGCGGAGGC GCAGCCGGGG 
GAGCCGGGCT GCAAAGCAGA GCGCCGTGCC CAGCACCTCG CCATCCCCGG CTTCTCCTTC 
GAGCAGTCGG ATGGCGGCGG CTCCCTGAGC AGCCTCTCAG ACCTGCTGGA CAGTGGGCGA 
TCCACACCGG ACTGCGAACT GAGAAAGCCG GAGCCCTTAA AAACTCAACG GAGCAACGCC 
GAGAAAAGCA AAGAGCTGCT CAGCGTGGCC AAGAGCCCCG TCCGGATGCA GGACTTGGTG 
ACTGTTGCAA CAGAAGAAGA CATCGACGAG CCCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149910.1
CDS	383..7621
Translation	MSDGESREPRPAGGEVRVPITVPPRRAAEQPLDDAGSAPSGSPSRDPPPDLASEEEEQVPYPALAPTAFFCLKQTTRPRSWCLRLVCNPWFEHVSMLVILLNCVTLGMFQPCEDVECQSERCTILEAFDDFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDRNLATTYNLTFLHPYYRTDEAEENPFICSSHRENGMQKCSNIPTRKEYKVECTLSMDSYTPSLYNPDFSSKNACINWNQYYNVCMAGDVNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTIRLYNNWQSKRRKKVNPNNTTNGQSRRGKKRITSIHHLIHHHHHHHHHHYHISNGSPRGPRSNPEICDLELKVMKPGGQLMLPSPSPNLQSPVPPPDSESVHSIYHADCHVEGPQVNCKSSNAPASIKLTTGLSNHGNMNYPTILPSPTSKASSVPVPKGKKNGSSPVAVVNSPVTLGTDSYGKLQQLVGEHGLRRTPSRLSGLSVACPLPSPPGGTLSCELQDCPFCASLLEDPEFAFSESDSCDSDSNGIYEFTQDLRHGDHRDQLQQQRGRRKRKKKKPKERNKVTRLWKAFGSKLKRIVESKYFNRGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSMFALEMLLKLLAFGLFGYIKNPYNIFDGIIVVISVWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSANFDDDFEKLKDLRATEMKMYSLAVTPNGHLEGRGSMPPPIIMRTAATPMPTPKCSPHMDSVHTFVDSRRGSNASLDPLTYDQKSSSSLRSSPCANWGTNSNWGSRRSSWNSLGRAPSLKKKNQSGERESLLSGEGKGSTDDDSDDAKSSTVSRPSLHRRAESLDYRSSLDLPELLQLPPMRHSLSISPMAVLPAEYQDCNGKMVHVPSEFFLHIDGHKEEAVDYEDDMEDSYCYRIRKVLEPYKPEWCKSHEDWSLYLFSPQNRFRVMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPHSTERIFLSVSNYIFTAIFVAEMMVKVVALGFFSGENTYLQSSWNVLDGVLVFVSIIDIIVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCDGPDVKNITTKTDCTNAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPIQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKEMYLSEAQRRPYYADYSPARKYIHTLCTSHYLDLFITFIIGVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAVLKLVAFGFRRFFKDRWNQLDLAIVLLSIVGITLEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCSEENPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIELEMSRGASTTSGSRGGAVALESRASCRSQEAMKSEPAAQAKHQALGGTAIPQPQDSQNMLTVRKISVSRMHSLPNDSYMFRPVMPAKAPFPLHEVEMEAYPCKSQRGSITSIRSQPVGTCSSLRVPPESLHVAVRSPGREGWHRWKVLPPHVVPRSPTLSKLLCRQEAVRTDSVDGHPPDPKDNLQAERGEMPPSPKHPQRTLTPSPLHPAASSLPGSPPRSPPTSPHRHPSLTRKHPCSQRGVPSPTESPPSPQGCLLEPSDLADEEVRHINSSAKDWGEHSEAGSCSTSPFVSPAASPVPLKNCSTSSLTAGSGKERDLKKFYSIDTKGFLTKPSWADDQRRHSIEICPSVRDGDCGFEERAEEKPRSPAHVQPESEYIHGARRKKKMSPPCISIDPPMEDDSGAASRTKPSENSMLRRRTPSCEFPAYREPLEPAEAQPGEPGCKAERRAQHLAIPGFSFEQSDGGGSLSSLSDLLDSGRSTPDCELRKPEPLKTQRSNAEKSKELLSVAKSPVRMQDLVTVATEEDIDEPV

Target ORF information:

RefSeq Version	XM_015294424.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294424.1

ORF Insert Sequence:

ATGAGTGATG GCGAGAGCCG AGAGCCGCGC CCGGCCGGCG GCGAGGTGCG GGTGCCCATC 
ACTGTGCCGC CCCGCCGCGC CGCCGAGCAG CCCCTGGACG ATGCCGGCTC CGCTCCCAGC 
GGTTCTCCCA GCCGCGACCC GCCGCCCGAC CTGGCCTCCG AGGAGGAGGA GCAGGTGCCT 
TACCCGGCGC TGGCACCCAC CGCTTTCTTC TGCCTCAAGC AAACCACCCG CCCGCGCAGC 
TGGTGCCTCA GGCTGGTCTG TAACCCCTGG TTCGAGCATG TCAGCATGCT GGTCATCCTC 
CTGAACTGTG TCACCCTGGG CATGTTCCAG CCCTGCGAGG ACGTGGAGTG CCAGTCGGAG 
CGCTGCACCA TCCTGGAGGC ATTTGACGAC TTCATTTTCG CTTTCTTTGC GGTGGAGATG 
GTCATCAAGA TGGTGGCTCT GGGGATCTTC GGGCAGAAGT GTTACCTTGG AGACACGTGG 
AATCGCCTGG ACTTCTTCAT CGTGATGGCA GGCATGATGG AGTACTCCCT GGATGGACAC 
AACGTCAGTC TGTCTGCCAT CCGCACCGTG CGCGTGCTGC GGCCGCTGCG AGCCATTAAC 
AGGGTGCCAA GTATGCGAAT ACTCGTCACC CTGCTGTTAG ATACTCTGCC TATGTTAGGC 
AACGTCCTCC TCTTGTGCTT CTTTGTTTTC TTCATCTTTG GGATCGTCGG TGTGCAGCTC 
TGGGCAGGGC TGCTGAGAAA CAGGTGTTTC CTGGACAGGA ATTTGGCAAC GACGTATAAC 
CTTACCTTCC TGCATCCGTA CTACCGCACA GACGAAGCAG AGGAGAATCC ATTTATCTGC 
TCATCCCATC GTGAGAACGG CATGCAGAAA TGCTCCAACA TCCCAACCAG GAAGGAGTAC 
AAGGTGGAGT GCACCTTGAG CATGGACTCC TACACCCCAA GTTTATACAA CCCTGACTTC 
AGCAGCAAGA ATGCCTGCAT AAACTGGAAC CAGTATTACA ACGTATGCAT GGCAGGGGAT 
GTAAACCCAC ACAATGGAGC TATTAATTTT GATAATATTG GCTATGCCTG GATAGCTATA 
TTTCAGGTTA TAACCCTGGA AGGATGGGTT GACATCATGT ATTATGTGAT GGATGCACAT 
TCTTTTTACA ATTTTATATA TTTTATATTG CTTATAATAG TTGGTTCCTT CTTCATGATT 
AACTTGTGCC TGGTTGTGAT AGCAACACAG TTCTCAGAAA CCAAGCAGCG GGAGCACCAG 
CTGATGCAGG AGCAGCGGGC CCGTTATCTC TCCAATGACA GCACAATTGC CAGCTTCTCC 
GAGCCAGGTA GCTGCTACGA GGAGCTGCTG AAGTACATCT GCCACATCTT CAGGAAGGTG 
AAGCGGCGGA CAATACGGCT GTACAATAAC TGGCAGAGCA AGCGCAGGAA AAAAGTTAAC 
CCGAACAACA CCACAAACGG GCAGAGCCGC CGAGGCAAAA AGCGCATCAC CTCCATTCAC 
CACCTCATCC ACCACCACCA CCACCACCAT CACCACCACT ACCACATCAG CAATGGGAGC 
CCGCGGGGGC CGCGCTCCAA CCCAGAGATC TGTGACCTGG AGCTCAAGGT GATGAAGCCT 
GGCGGCCAGC TGATGCTCCC TTCTCCATCA CCCAACTTGC AGAGCCCCGT CCCTCCTCCA 
GATTCGGAGT CTGTGCATAG CATTTACCAT GCGGACTGTC ACGTTGAAGG ACCACAAGTG 
AACTGTAAGT CTTCCAATGC CCCTGCAAGC ATTAAACTCA CTACTGGGCT CTCCAACCAT 
GGCAACATGA ATTATCCTAC CATCCTGCCT TCACCAACTA GCAAAGCCAG TTCTGTCCCA 
GTTCCCAAAG GAAAGAAGAA TGGCAGTTCA CCTGTGGCTG TAGTTAACAG CCCTGTAACT 
TTGGGCACGG ATTCTTATGG GAAGCTGCAG CAACTGGTAG GAGAGCATGG TCTGCGGAGG 
ACACCCAGCC GGCTGTCGGG GCTGAGCGTG GCCTGCCCAC TGCCCAGCCC GCCGGGCGGC 
ACGCTGAGCT GCGAGCTGCA GGACTGCCCC TTCTGCGCCA GCCTCCTGGA GGACCCCGAG 
TTCGCCTTCA GTGAGTCTGA CAGCTGCGAC TCCGACAGCA ACGGCATCTA CGAGTTCACC 
CAGGACCTGA GGCACGGCGA CCACAGGGAC CAGCTGCAGC AGCAGCGGGG CAGGAGGAAG 
AGGAAGAAGA AGAAACCCAA GGAGAGGAAC AAGGTCACCC GCCTGTGGAA GGCGTTCGGC 
AGCAAGCTGA AGAGGATCGT GGAGAGCAAG TACTTCAACC GAGGGATCAT GATCGCCATC 
CTCATCAACA CGCTCAGCAT GGGCATCGAG TACCACGAGC AGCCAGACGA GCTGACCAAC 
GCCTTGGAGA TCAGTAACAT CGTCTTCACA AGCATGTTCG CCCTGGAGAT GCTCCTGAAA 
CTCCTTGCCT TTGGCCTCTT CGGCTACATC AAGAACCCAT ACAACATCTT TGACGGGATC 
ATAGTTGTCA TCAGTGTCTG GGAGATCATT GGGCAGTCGG ACGGTGGGCT TTCCGTGCTC 
CGAACTTTCC GGCTCCTGCG GGTGCTGAAG TTGGTGCGCT TCATGCCGGC CCTGCGCCGC 
CAGCTTGTGG TCCTGATGAA GACTATGGAC AACGTAGCCA CCTTCTGCAT GTTGCTCATG 
CTCTTCATCT TTATCTTCAG CATTCTCGGC ATGCATCTTT TTGGATGCAA GTTCAGTCTG 
AAGACTGATA CAGGAGACAC AGTTCCAGAT AGGAAGAATT TTGATTCCTT ACTCTGGGCC 
ATTGTGACAG TATTTCAGAT CCTCACTCAA GAGGACTGGA ATGTGGTCCT GTACAACGGG 
ATGGCATCAA CATCTTCATG GGCAGCACTC TACTTTGTGG CCCTGATGAC CTTCGGCAAT 
TATGTGCTCT TCAACCTTCT GGTTGCCATT CTGGTGGAAG GCTTCCAGGC AGAGGGGGAT 
GCCAATAGGT CAGACACAGA CGAGGACAAG ACATCCGCCA ACTTTGATGA TGATTTTGAG 
AAGCTGAAAG ACCTCCGTGC AACGGAGATG AAGATGTACT CACTTGCTGT CACCCCGAAT 
GGGCACCTGG AGGGCAGGGG CAGCATGCCA CCCCCTATAA TCATGCGCAC CGCTGCCACT 
CCGATGCCCA CACCGAAGTG TTCCCCGCAC ATGGATTCAG TGCACACCTT TGTGGACTCG 
AGGAGAGGGA GTAATGCTTC TCTAGATCCC CTGACCTACG ATCAGAAGTC CTCGTCTAGC 
CTCCGCAGTT CCCCTTGTGC CAACTGGGGT ACCAACAGCA ACTGGGGAAG CCGCCGCTCA 
AGCTGGAACA GCCTGGGCAG AGCCCCGAGC CTCAAGAAGA AGAACCAATC TGGTGAAAGA 
GAGTCTTTGC TCTCCGGGGA GGGGAAAGGC AGCACAGACG ATGACTCCGA TGACGCCAAG 
TCCAGCACGG TCAGCAGGCC CTCACTGCAC CGCCGGGCAG AGTCCTTGGA TTACCGCAGC 
TCCCTAGACC TGCCCGAGCT GCTGCAGCTG CCCCCCATGC GCCATTCGCT CAGTATCAGC 
CCCATGGCTG TTCTGCCTGC TGAGTACCAA GACTGCAACG GCAAGATGGT GCACGTCCCC 
AGCGAGTTCT TCCTGCACAT CGATGGCCAC AAGGAAGAGG CCGTGGACTA TGAGGATGAC 
ATGGAGGATA GTTACTGCTA CCGGATTCGT AAAGTCCTGG AGCCCTACAA ACCAGAGTGG 
TGCAAGAGTC ACGAGGATTG GTCCCTCTAC TTGTTTTCCC CACAGAATCG GTTCAGAGTG 
ATGTGCCAGA AGGTCATTGC CCACAAAATG TTTGATCATG TGGTACTGGT CTTCATATTT 
CTCAACTGCA TTACAATAGC ACTGGAGCGA CCAGACATAG ATCCGCACAG CACCGAGAGG 
ATATTCCTGA GTGTTTCTAA TTACATCTTC ACTGCAATCT TTGTGGCTGA GATGATGGTT 
AAGGTAGTGG CTCTTGGCTT TTTCTCTGGG GAGAACACCT ATCTACAGAG CAGCTGGAAC 
GTCCTGGATG GAGTCCTGGT CTTCGTATCT ATCATAGACA TTATTGTCTC CATGGCATCA 
GCAGGAGGAG CAAAGATCCT GGGTGTCCTT CGCGTTTTGA GACTGCTGCG GACCTTGAGA 
CCTCTCCGAG TCATCAGCAG AGCCCCAGGT CTGAAGCTGG TGGTGGAAAC CTTGATCTCC 
TCCTTGAGGC CTATTGGGAA TATTGTTCTC ATCTGCTGTG CTTTCTTCAT TATCTTTGGG 
ATTTTAGGGG TGCAGCTTTT TAAAGGCAAA TTCTACTACT GTGACGGTCC CGATGTGAAA 
AACATCACAA CGAAGACTGA CTGCACCAAT GCACACTACA GATGGGTTCG GCGGAAGTAC 
AATTTTGACA ATTTGGGACA GGCCCTCATG TCCTTGTTTG TCCTTTCCTC CAAAGATGGC 
TGGGTGAACA TCATGTACGA TGGTCTGGAT GCTGTGGGTA TTGACCAACA GCCCATCCAG 
AACCATAACC CTTGGATGCT TCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG GGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG 
GAGGCTGAGG AGGCCCGGCG GCGGGAGGAG AAGCGTCTGA GACGCCTGGA GAAAAAGCGC 
AGGAGTAAGG AGATGTACCT GTCTGAAGCC CAGCGCAGGC CTTACTATGC TGACTATTCT 
CCAGCACGGA AATACATTCA CACCCTCTGC ACCAGCCACT ATCTCGACCT CTTCATCACC 
TTCATCATTG GAGTCAACGT CATCACTATG TCGATGGAGC ACTACAACCA GCCAAAGTCC 
CTGGATGAAG CCCTGAAGTA CTGTAACTAC GTGTTCACCA TAGTTTTTGT GTTCGAGGCA 
GTCCTGAAGT TGGTGGCATT TGGTTTTCGA AGGTTCTTTA AGGACAGGTG GAATCAGCTG 
GATTTGGCCA TCGTTCTCTT ATCCATTGTG GGAATCACGC TGGAGGAAAT TGAGATGAAC 
GCTGCACTGC CCATCAATCC CACAATAATA CGCATCATGC GGGTGCTGAG AATAGCAAGA 
GTGCTGAAAC TGCTTAAAAT GGCCACCGGA ATGCGAGCCC TCCTGGACAC AGTGGTACAA 
GCGCTTCCCC AGGTAGGGAA CCTTGGCCTA CTTTTTATGC TCCTGTTTTT TATCTATGCT 
GCCCTGGGAG TGGAATTGTT TGGCAAGCTA GACTGCAGCG AAGAGAATCC TTGCGAAGGG 
CTGAGCCGGC ACGCCACGTT CACCAACTTT GGGATGGCCT TCCTCACCCT CTTCAGGGTG 
TCCACGGGGG ACAACTGGAA CGGTATCATG AAGGACACTC TCCGGGAATG CACGCGGGAA 
GACAAGCACT GCCTCAGCTA CCTGCCTGTT ATCTCTCCTG TCTACTTCGT CACCTTCGTC 
CTCATCGCAC AGTTCGTCCT GGTCAACGTG GTGGTGGCCG TGCTGATGAA GCACTTGGAG 
GAGAGCAACA AGGAGGCCAA GGAGGACGCT GAGATGGACG CTGAGATCGA GCTGGAGATG 
TCCCGAGGAG CGAGCACCAC CAGTGGCAGC AGGGGAGGTG CGGTGGCCCT GGAGAGCAGA 
GCATCCTGCA GAAGCCAGGA GGCCATGAAG TCAGAGCCAG CAGCGCAGGC GAAGCACCAA 
GCCCTGGGAG GCACGGCCAT CCCGCAGCCT CAGGACTCCC AGAACATGCT GACGGTCCGC 
AAGATCTCCG TTTCACGCAT GCACTCCTTG CCCAACGACA GCTACATGTT CCGGCCCGTG 
ATGCCAGCCA AAGCTCCCTT TCCCCTCCAC GAGGTGGAGA TGGAGGCATA TCCCTGCAAG 
TCACAGCGAG GGTCCATCAC CTCCATCCGC TCGCAGCCCG TGGGGACGTG CTCCTCCTTG 
AGGGTCCCCC CCGAGTCCCT CCACGTGGCC GTGCGCTCCC CCGGCCGTGA GGGCTGGCAC 
CGCTGGAAGG TCCTTCCCCC GCACGTCGTC CCTCGGTCTC CTACTCTCAG CAAGCTGCTG 
TGCCGGCAGG AGGCCGTTCG GACAGACTCG GTGGACGGAC ACCCTCCAGA CCCCAAGGAC 
AACCTGCAAG CAGAGCGTGG AGAGATGCCA CCATCACCAA AGCACCCTCA GCGCACCCTC 
ACTCCCAGCC CTCTCCACCC TGCAGCCTCC TCCCTGCCGG GCTCCCCTCC GCGCTCCCCA 
CCAACCTCCC CACACCGGCA CCCATCGCTG ACTCGGAAAC ACCCCTGCAG CCAGCGCGGG 
GTCCCCAGTC CCACCGAGAG CCCCCCAAGC CCCCAGGGCT GCCTCCTGGA GCCCTCTGAC 
CTGGCTGACG AGGAGGTTCG CCACATCAAC AGCTCAGCCA AAGACTGGGG GGAGCACTCG 
GAGGCCGGGA GCTGCTCCAC GTCGCCGTTC GTCTCGCCGG CCGCGTCGCC GGTGCCCCTG 
AAGAATTGCA GCACAAGCAG CCTGACGGCA GGCAGCGGCA AGGAGAGGGA CTTGAAGAAG 
TTTTACAGCA TCGATACGAA GGGCTTCCTC ACCAAGCCCA GCTGGGCGGA TGACCAAAGG 
CGGCACTCCA TCGAGATCTG CCCCTCCGTC CGCGATGGGG ACTGCGGCTT CGAGGAGCGC 
GCCGAGGAAA AGCCACGCTC CCCCGCCCAC GTCCAGCCAG AGTCCGAGTA CATCCACGGA 
GCCCGGAGGA AGAAGAAGAT GAGCCCGCCC TGCATTTCTA TAGATCCTCC CATGGAGGAC 
GACAGCGGGG CGGCCTCGCG CACCAAACCC TCCGAGAACA GCATGCTGCG CCGCAGGACC 
CCGTCCTGCG AGTTCCCGGC GTACCGAGAG CCCCTGGAGC CGGCGGAGGC GCAGCCGGGG 
GAGCCGGGCT GCAAAGCAGA GCGCCGTGCC CAGCACCTCG CCATCCCCGG CTTCTCCTTC 
GAGCAGTCGG ATGGCGGCGG CTCCCTGAGC AGCCTCTCAG ACCTGCTGGA CAGTGGGCGA 
TCCACACCGG ACTGCGAACT GAGAAAGCCG GAGCCCTTAA AAACTCAACG GAGCAACGCC 
GAGAAAAGCA AAGAGCTGCT CAGCGTGGCC AAGAGCCCCG TCCGGATGCA GGACTTGGTG 
ACTGTTGCAA CAGAAGAAGA CATCGACGAG CCCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CACNA1H cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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