Prss21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prss21
Entrez Gene ID	57256
Full Name	protease, serine 21
Synonyms	1700023E12Rik,TESP5,mT4,testisin
General protein information	Preferred Names protease, serine 21 Names testisin serine protease 21 testicular serine protease 5 tryptase 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 12.11 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246542.2	XP_011244844.1	testisin isoform X1
NM_020487.4	NP_065233.2	testisin precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prss21	NP_852142.1
Macaca mulatta (Rhesus monkey)	PRSS21	XP_001087762.2
Canis lupus familiaris (dog)	PRSS21	XP_536999.3
Homo sapiens (human)	PRSS21	NP_659205.1
Pan troglodytes (chimpanzee)	PRSS21	XP_510754.3
Bos taurus (cattle)	PRSS21	XP_002697910.3
Mus musculus (house mouse)	Prss21	NP_065233.2

Gene Ontology
Bibliography

Related articles in PubMed

Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.
Asano A, Nelson-Harrington JL, Travis AJ
The Journal of biological chemistry288(39)28104-15(2013 Sep)

Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization.
Zhou C, Kang W, Baba T
The Journal of reproduction and development58(3)330-7(2012)

Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro.
Kawano N, Kang W, Yamashita M, Koga Y, Yamazaki T, Hata T, Miyado K, Baba T
Biology of reproduction83(3)359-69(2010 Sep)

The glycosylphosphatidylinositol-anchored serine protease PRSS21 (testisin) imparts murine epididymal sperm cell maturation and fertilizing ability.
Netzel-Arnett S, Bugge TH, Hess RA, Carnes K, Stringer BW, Scarman AL, Hooper JD, Tonks ID, Kay GF, Antalis TM
Biology of reproduction81(5)921-32(2009 Nov)

Reduced fertility of mouse epididymal sperm lacking Prss21/Tesp5 is rescued by sperm exposure to uterine microenvironment.
Yamashita M, Honda A, Ogura A, Kashiwabara S, Fukami K, Baba T
Genes to cells : devoted to molecular & cellular mechanisms13(10)1001-13(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prss21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prss21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12009	NM_020487.4 Latest version!	Mus musculus protease, serine 21 (Prss21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu69718	XM_011246542.2 Latest version!	Mus musculus protease, serine 21 (Prss21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prss21 ( NM_020487.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_065233.2 Mus musculus protease, serine 21 (Prss21), mRNA.

Price & Availability↑

CloneID	OMu12009
Clone ID Related Accession (Same CDS sequence)	NM_020487.4
Accession Version	NM_020487.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Mus musculus(house mouse)
Product	testisin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC049588.1. On Aug 14, 2009 this sequence version replaced NM_020487.3. ##Evidence-Data-START## Transcript exon combination :: BC049588.1, AB033822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCGC GGGGCAAGAC GTTGGTGCCA CTGCTGGTGG TTGTGGCGAC TGCGGCGATG 
GCCTTACAGT CAACCTATTT GCAGGTGGAT CCTGAGAAAC CCGAACTGCA GGAACCCGAC 
CTATTGTCAG GGCCCTGCGG TCACAGGACC ATCCCTTCCC GTATAGTGGG TGGCGATGAT 
GCTGAGCTTG GCCGCTGGCC GTGGCAAGGG AGCCTGCGTG TATGGGGCAA CCACTTATGT 
GGCGCAACCT TGCTCAACCG CCGCTGGGTG CTTACAGCTG CCCACTGCTT CCAAAAGGAT 
AACGATCCTT TTGACTGGAC AGTCCAGTTT GGTGAGCTGA CATCCAGGCC ATCTCTCTGG 
AACCTACAGG CCTATTCCAA CCGTTACCAA ATAGAAGATA TTTTCCTGAG CCCCAAGTAC 
TCGGAGCAGT ATCCCAATGA CATAGCCCTG CTGAAGCTGT CATCTCCAGT CACCTACAAT 
AACTTCATCC AGCCCATCTG CCTCCTGAAC TCCACGTACA AGTTTGAGAA CCGAACTGAC 
TGCTGGGTGA CCGGCTGGGG GGCTATTGGA GAAGATGAGA GTCTGCCATC TCCCAACACT 
CTCCAGGAAG TGCAGGTAGC TATTATCAAC AACAGCATGT GTAACCATAT GTACAAAAAG 
CCAGACTTCC GCACGAACAT CTGGGGAGAC ATGGTTTGCG CTGGCACTCC TGAAGGTGGC 
AAGGATGCCT GCTTTGGTGA CTCGGGAGGA CCCTTGGCCT GCGACCAGGA TACGGTGTGG 
TATCAGGTTG GAGTTGTGAG CTGGGGAATA GGCTGTGGTC GCCCCAATCG CCCTGGAGTC 
TATACCAACA TCAGTCATCA CTACAACTGG ATCCAGTCAA CCATGATCCG CAATGGGCTG 
CTCAGGCCTG ACCCAGTCCC CCTGCTACTG TTTCTCACTC TGGCCTGGGC TTCCTCTTTG 
CTGAGGCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065233.2
CDS	24..998
Translation	MGARGKTLVPLLVVVATAAMALQSTYLQVDPEKPELQEPDLLSGPCGHRTIPSRIVGGDDAELGRWPWQGSLRVWGNHLCGATLLNRRWVLTAAHCFQKDNDPFDWTVQFGELTSRPSLWNLQAYSNRYQIEDIFLSPKYSEQYPNDIALLKLSSPVTYNNFIQPICLLNSTYKFENRTDCWVTGWGAIGEDESLPSPNTLQEVQVAIINNSMCNHMYKKPDFRTNIWGDMVCAGTPEGGKDACFGDSGGPLACDQDTVWYQVGVVSWGIGCGRPNRPGVYTNISHHYNWIQSTMIRNGLLRPDPVPLLLFLTLAWASSLLRPA

Target ORF information:

RefSeq Version	NM_020487.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 21 (Prss21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020487.4

ORF Insert Sequence:

ATGGGCGCGC GGGGCAAGAC GTTGGTGCCA CTGCTGGTGG TTGTGGCGAC TGCGGCGATG 
GCCTTACAGT CAACCTATTT GCAGGTGGAT CCTGAGAAAC CCGAACTGCA GGAACCCGAC 
CTATTGTCAG GGCCCTGCGG TCACAGGACC ATCCCTTCCC GTATAGTGGG TGGCGATGAT 
GCTGAGCTTG GCCGCTGGCC GTGGCAAGGG AGCCTGCGTG TATGGGGCAA CCACTTATGT 
GGCGCAACCT TGCTCAACCG CCGCTGGGTG CTTACAGCTG CCCACTGCTT CCAAAAGGAT 
AACGATCCTT TTGACTGGAC AGTCCAGTTT GGTGAGCTGA CATCCAGGCC ATCTCTCTGG 
AACCTACAGG CCTATTCCAA CCGTTACCAA ATAGAAGATA TTTTCCTGAG CCCCAAGTAC 
TCGGAGCAGT ATCCCAATGA CATAGCCCTG CTGAAGCTGT CATCTCCAGT CACCTACAAT 
AACTTCATCC AGCCCATCTG CCTCCTGAAC TCCACGTACA AGTTTGAGAA CCGAACTGAC 
TGCTGGGTGA CCGGCTGGGG GGCTATTGGA GAAGATGAGA GTCTGCCATC TCCCAACACT 
CTCCAGGAAG TGCAGGTAGC TATTATCAAC AACAGCATGT GTAACCATAT GTACAAAAAG 
CCAGACTTCC GCACGAACAT CTGGGGAGAC ATGGTTTGCG CTGGCACTCC TGAAGGTGGC 
AAGGATGCCT GCTTTGGTGA CTCGGGAGGA CCCTTGGCCT GCGACCAGGA TACGGTGTGG 
TATCAGGTTG GAGTTGTGAG CTGGGGAATA GGCTGTGGTC GCCCCAATCG CCCTGGAGTC 
TATACCAACA TCAGTCATCA CTACAACTGG ATCCAGTCAA CCATGATCCG CAATGGGCTG 
CTCAGGCCTG ACCCAGTCCC CCTGCTACTG TTTCTCACTC TGGCCTGGGC TTCCTCTTTG 
CTGAGGCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prss21 ( XM_011246542.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244844.1 Mus musculus protease, serine 21 (Prss21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69718
Clone ID Related Accession (Same CDS sequence)	XM_011246542.2
Accession Version	XM_011246542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	testisin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGA AGACAGTGTT AGGACACCAC GGTCCCCAGA CAATCCTTGG GCCTAGGGAT 
TGGCGTCTTC TTCAGAGTGG GCGCGGGCCT GGCGTAGCAG GAAGTAAGAG GGGGTGCCAG 
GTGGCAGGAG AGGTGGCCAT GGGCGCGCGG GGCAAGACGT TGGTGCCACT GCTGGTGGTT 
GTGGCGACTG CGGCGATGGC CTTACAGTCA ACCTATTTGC AGGTGGATCC TGAGAAACCC 
GAACTGCAGG AACCCGACCT ATTGTCAGGG CCCTGCGGTC ACAGGACCAT CCCTTCCCGT 
ATAGTGGGTG GCGATGATGC TGAGCTTGGC CGCTGGCCGT GGCAAGGGAG CCTGCGTGTA 
TGGGGCAACC ACTTATGTGG CGCAACCTTG CTCAACCGCC GCTGGGTGCT TACAGCTGCC 
CACTGCTTCC AAAAGGATAA CGATCCTTTT GACTGGACAG TCCAGTTTGG TGAGCTGACA 
TCCAGGCCAT CTCTCTGGAA CCTACAGGCC TATTCCAACC GTTACCAAAT AGAAGATATT 
TTCCTGAGCC CCAAGTACTC GGAGCAGTAT CCCAATGACA TAGCCCTGCT GAAGCTGTCA 
TCTCCAGTCA CCTACAATAA CTTCATCCAG CCCATCTGCC TCCTGAACTC CACGTACAAG 
TTTGAGAACC GAACTGACTG CTGGGTGACC GGCTGGGGGG CTATTGGAGA AGATGAGAGT 
GAGGCTGGGT CTGCCATCTC CCAACACTCT CCAGGAAGTG CAGGTAGCTA TTATCAACAA 
CAGCATGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244844.1
CDS	90..881
Translation	MPWKTVLGHHGPQTILGPRDWRLLQSGRGPGVAGSKRGCQVAGEVAMGARGKTLVPLLVVVATAAMALQSTYLQVDPEKPELQEPDLLSGPCGHRTIPSRIVGGDDAELGRWPWQGSLRVWGNHLCGATLLNRRWVLTAAHCFQKDNDPFDWTVQFGELTSRPSLWNLQAYSNRYQIEDIFLSPKYSEQYPNDIALLKLSSPVTYNNFIQPICLLNSTYKFENRTDCWVTGWGAIGEDESEAGSAISQHSPGSAGSYYQQQHV

Target ORF information:

RefSeq Version	XM_011246542.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 21 (Prss21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246542.2

ORF Insert Sequence:

ATGCCCTGGA AGACAGTGTT AGGACACCAC GGTCCCCAGA CAATCCTTGG GCCTAGGGAT 
TGGCGTCTTC TTCAGAGTGG GCGCGGGCCT GGCGTAGCAG GAAGTAAGAG GGGGTGCCAG 
GTGGCAGGAG AGGTGGCCAT GGGCGCGCGG GGCAAGACGT TGGTGCCACT GCTGGTGGTT 
GTGGCGACTG CGGCGATGGC CTTACAGTCA ACCTATTTGC AGGTGGATCC TGAGAAACCC 
GAACTGCAGG AACCCGACCT ATTGTCAGGG CCCTGCGGTC ACAGGACCAT CCCTTCCCGT 
ATAGTGGGTG GCGATGATGC TGAGCTTGGC CGCTGGCCGT GGCAAGGGAG CCTGCGTGTA 
TGGGGCAACC ACTTATGTGG CGCAACCTTG CTCAACCGCC GCTGGGTGCT TACAGCTGCC 
CACTGCTTCC AAAAGGATAA CGATCCTTTT GACTGGACAG TCCAGTTTGG TGAGCTGACA 
TCCAGGCCAT CTCTCTGGAA CCTACAGGCC TATTCCAACC GTTACCAAAT AGAAGATATT 
TTCCTGAGCC CCAAGTACTC GGAGCAGTAT CCCAATGACA TAGCCCTGCT GAAGCTGTCA 
TCTCCAGTCA CCTACAATAA CTTCATCCAG CCCATCTGCC TCCTGAACTC CACGTACAAG 
TTTGAGAACC GAACTGACTG CTGGGTGACC GGCTGGGGGG CTATTGGAGA AGATGAGAGT 
GAGGCTGGGT CTGCCATCTC CCAACACTCT CCAGGAAGTG CAGGTAGCTA TTATCAACAA 
CAGCATGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.
The Journal of biological chemistry288(39)28104-15(2013 Sep)
Asano A,Nelson-Harrington JL,Travis AJ

Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization.
The Journal of reproduction and development58(3)330-7(2012)
Zhou C,Kang W,Baba T

Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro.
Biology of reproduction83(3)359-69(2010 Sep)
Kawano N,Kang W,Yamashita M,Koga Y,Yamazaki T,Hata T,Miyado K,Baba T

The glycosylphosphatidylinositol-anchored serine protease PRSS21 (testisin) imparts murine epididymal sperm cell maturation and fertilizing ability.
Biology of reproduction81(5)921-32(2009 Nov)
Netzel-Arnett S,Bugge TH,Hess RA,Carnes K,Stringer BW,Scarman AL,Hooper JD,Tonks ID,Kay GF,Antalis TM

Reduced fertility of mouse epididymal sperm lacking Prss21/Tesp5 is rescued by sperm exposure to uterine microenvironment.
Genes to cells : devoted to molecular & cellular mechanisms13(10)1001-13(2008 Oct)
Yamashita M,Honda A,Ogura A,Kashiwabara S,Fukami K,Baba T