Nlrc4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrc4
Entrez Gene ID	268973
Full Name	NLR family, CARD domain containing 4
Synonyms	9530011P19Rik,CLAN,CLAN1,CLANA,CLANB,CLANC,CLAND,Card12,IPAF
General protein information	Preferred Names NLR family, CARD domain containing 4 Names NLR family CARD domain-containing protein 4 caspase recruitment domain family, member 12 caspase recruitment domain-containing protein 12 ice protease-activating factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524346.3	XP_006524409.1	NLR family CARD domain-containing protein 4 isoform X1
XM_006524346.4	XP_006524409.1	NLR family CARD domain-containing protein 4 isoform X1
XM_006524347.4	XP_006524410.1	NLR family CARD domain-containing protein 4 isoform X2
XM_006524347.3	XP_006524410.1	NLR family CARD domain-containing protein 4 isoform X2
XM_017317490.1	XP_017172979.1	NLR family CARD domain-containing protein 4 isoform X1
XM_017317491.1	XP_017172980.1	NLR family CARD domain-containing protein 4 isoform X1
NM_001033367.3	NP_001028539.1	NLR family CARD domain-containing protein 4

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NLRC4	XP_001105818.2
Bos taurus (cattle)	NLRC4	NP_001179252.2
Homo sapiens (human)	NLRC4	NP_001186067.1
Pan troglodytes (chimpanzee)	NLRC4	XP_515390.2
Xenopus tropicalis (tropical clawed frog)	nlrc4	XP_004914732.1
Mus musculus (house mouse)	Nlrc4	NP_001028539.1
Rattus norvegicus (Norway rat)	Nlrc4	XP_003750157.2

Gene Ontology
Bibliography

Related articles in PubMed

The Nlrp6 inflammasome is not required for baseline colonic inner mucus layer formation or function.
Volk JK, Nyström EEL, van der Post S, Abad BM, Schroeder BO, Johansson Å, Svensson F, Jäverfelt S, Johansson MEV, Hansson GC, Birchenough GMH
The Journal of experimental medicine216(11)2602-2618(2019 Nov)

NLRC4 suppresses IL-17A-mediated neutrophil-dependent host defense through upregulation of IL-18 and induction of necroptosis during Gram-positive pneumonia.
Paudel S, Ghimire L, Jin L, Baral P, Cai S, Jeyaseelan S
Mucosal immunology12(1)247-257(2019 01)

Autoinflammatory mutation in NLRC4 reveals a leucine-rich repeat (LRR)-LRR oligomerization interface.
Moghaddas F, Zeng P, Zhang Y, Schützle H, Brenner S, Hofmann SR, Berner R, Zhao Y, Lu B, Chen X, Zhang L, Cheng S, Winkler S, Lehmberg K, Canna SW, Czabotar PE, Wicks IP, De Nardo D, Hedrich CM, Zeng H, Masters SL
The Journal of allergy and clinical immunology142(6)1956-1967.e6(2018 12)

IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.
Karki R, Lee E, Place D, Samir P, Mavuluri J, Sharma BR, Balakrishnan A, Malireddi RKS, Geiger R, Zhu Q, Neale G, Kanneganti TD
Cell173(4)920-933.e13(2018 05)

ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Lee BL, Mirrashidi KM, Stowe IB, Kummerfeld SK, Watanabe C, Haley B, Cuellar TL, Reichelt M, Kayagaki N
Scientific reports8(1)3788(2018 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nlrc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53643	XM_006524347.4 Latest version!	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu19575	NM_001033367.3 Latest version!	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu19575	XM_017317491.1 Latest version!	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu19575	XM_006524346.4 Latest version!	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu19575	XM_017317490.1 Latest version!	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu19575	XM_006524346.3	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu53643	XM_006524347.3	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrc4 ( XM_006524347.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524410.1 Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu53643
Clone ID Related Accession (Same CDS sequence)	XM_006524347.4 , XM_006524347.3
Accession Version	XM_006524347.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	NLR family CARD domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524347.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGA AGGACCATCG TCATCACCGT GTGGAGCAGC TGACTTTGGG CAGCCTGCTC 
GAGGCTCTGA AGAGCCCCTG CCTGATTGAA GGCGAGTCTG GCAAAGGGAA GTCCACCCTG 
CTGCAGAGAA TCGCTATGCT CTGGGCCTCT GGGGGCTGCA GGGCTCTGAA GGGGTTCAGA 
TTAGTCTTCT TCATCCACCT GAGAAGCGCC AGGGGGGGAC TATTCGAAAC ACTGTACGAT 
CAGCTCCTGA ACATACCCGA CTTCATCAGC AAGCCGACCT TCAAGGCTCT GCTGCTGAAG 
CTACACAAGG AGGTCCTCTT TCTTCTCGAT GGTTACAATG AATTCCATCC CCAGAACTGC 
CCAGAAATTG AAGCCCTGAT AAAGGAAAAC CATCGCTTCA AGAACATGGT CATTGTCACC 
ACCACCACGG AGTGCCTGAG GCATATCAGA CATGTTGGCG CCCTGACTGC GGAGGTGGGA 
GATATGACCG AAGACAGTGC CAAAGATCTC ATCGAGGCAG TGCTGGTACC TGATCAGGTT 
GAACGCCTGT GGGCCCAAAT CCAGGAGTCC AGGTGCCTGA GAAATCTGAT GAAGACCCCT 
CTCTTCGTGG TGATCACCTG TGCAATTCAG ATGGGCAGAC AGGAATTCCA AGCTCACACC 
CAAACCATGC TGTTCCAAAC CTTCTACGAC CTCCTGATAC AGAAAAACAG CCACAGATAT 
AGAGGTGGAG CTTCAGGTGA TTTTGCCAGG AGCCTAGACT ACTGTGGAGA CCTGGCCCTA 
GAAGGTGTGT TCGCCCACAA ATTTGATTTT GAACCCGAGC ATGGGTCCAG CATGAACGAG 
GACGTCCTGG TGACAATAGG GCTCCTCTGT AAGTACACAG CTCAGAGGCT GAAGCCCACG 
TATAAATTCT TTCATAAATC ATTTCAGGAG TACACGGCAG GTCGGAGACT CAGCAGTTTG 
CTGACGTCCA AAGAGCCAGA GGAGGTGAGC AAAGGGAACA GCTACTTAAA CAAAATGGTT 
TCCATCTCTG ACATCACATC CCTATATGGC AATCTGCTCC TCTACACGTG TGGGTCGTCC 
ACAGAAGCAA CCAGGGCGGT CATGAGGCAC CTTGCAATGG TTTATCAGCA CGGCAGCCTA 
CAAGGACTTT CAGTCACCAA GAGGCCTCTC TGGAGGCAGG AATCAATCCA GAGTCTGAGA 
AATACCACTG AGCAAGATGT TCTGAAAGCC ATCAATGTAA ATTCCTTCGT AGAGTGTGGC 
ATCAATTTGT TCTCAGAGAG TATGTCTAAA TCAGACCTGA GCCAAGAATT TGAAGCTTTC 
TTTCAAGGTA AAAGTTTATA CATCAACTCA GAGAACATCC CTGACTATTT ATTTGACTTC 
TTTGAATACT TGCCTAATTG TGCAAGCGCA TTGGACTTCG TGAAGTTGGA TTTCTATGAA 
AGAGCTACAG AGTCACAGGA CAAGGCAGAA GAGAATGTCC CTGGAGTTCA CACAGAAGGG 
CCCTCAGAAA CCTACATTCC CCCCAGGGCT GTGTCTTTGT TCTTCAACTG GAAGCAGGAA 
TTCAAGACTC TAGAGGTCAC ACTCCGAGAT ATTAACAAGT TGAATAAGCA AGATATCAAA 
TATCTGGGGA AGATATTCAG CTCTGCCACC AACCTCCGGC TGCATATCAA GAGATGTGCA 
GCCATGGCTG GAAGACTCAG CTCAGTCCTC AGAACCTGCA AGAACATGCA TACCCTCATG 
GTGGAAGCCA GTCCCCTCAC CACGGATGAC GAACAGTACA TCACATCTGT GACAGGCCTC 
CAGAACTTAA GTATTCACCG CTTGCACACT CAACAGCTGC CAGGTGGTCT GATTGACAGC 
TTGGGTAATC TGAAGAACCT CGAGAGACTC ATACTGGATG ACATCAGGAT GAACGAGGAA 
GATGCTAAAA ACCTAGCCGA AGGCCTACGA AGCCTGAAGA AGATGCGTTT ACTCCATTTG 
ACTCATTTGT CTGACATTGG GGAGGGGATG GACTACATAG TCAAGTCTCT CTCAGAAGAA 
TCCTGTGATC TCCAAGAGAT GAAGTTGGTG GCCTGCTGTC TGACTGCAAA CTCTGTGAAA 
GTTCTAGCAC AGAATCTTCA CAATTTGATC AAGCTGAGCA TTCTTGATAT ATCAGAAAAT 
TACCTGGAAA AGGATGGGAA TGAAGCTCTA CAGGAACTGA TCGGCAGGCT TGGCGTTCTG 
GGAGAGCTCA CTACATTGAT GCTGCCTTGG TGCTGGGATG TGCACACCAG CCTGCCCAAG 
CTGTTGAAGC AGTTGGAGGG GACCCCAGGA CTTGCCAAAC TTGGATTGAA AAACTGGAGA 
CTCAGAGACG AAGAGATTAA AAGTTTAGGT GAATTTCTGG AGATGAATCC TCTGAGAGAC 
TTGCAGCAGT TGGATTTAGC GGGGCACTGT GTGAGCAGTG ACGGATGGCT TTACTTCATG 
AATGTGTTTG AGAATCTGAA GCAGTTAGTG TTTTTTGACT TTAGCACTGA GGAGTTCTTA 
CCGGATGCAG CACTGGTGAG GAAACTTAGT CAAGTGTTAT CCAAGTTAAC TCTTCTGCAA 
GAGGTAAAGC TCACGGGCTG GGAGTTTGAT GACTATGATA TTAGCGCTAT TAAAGGCACC 
TTTAAACTAG TGACTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524410.1
CDS	650..3310
Translation	MWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA

Target ORF information:

RefSeq Version	XM_006524347.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524347.4

ORF Insert Sequence:

ATGTGGAAGA AGGACCATCG TCATCACCGT GTGGAGCAGC TGACTTTGGG CAGCCTGCTC 
GAGGCTCTGA AGAGCCCCTG CCTGATTGAA GGCGAGTCTG GCAAAGGGAA GTCCACCCTG 
CTGCAGAGAA TCGCTATGCT CTGGGCCTCT GGGGGCTGCA GGGCTCTGAA GGGGTTCAGA 
TTAGTCTTCT TCATCCACCT GAGAAGCGCC AGGGGGGGAC TATTCGAAAC ACTGTACGAT 
CAGCTCCTGA ACATACCCGA CTTCATCAGC AAGCCGACCT TCAAGGCTCT GCTGCTGAAG 
CTACACAAGG AGGTCCTCTT TCTTCTCGAT GGTTACAATG AATTCCATCC CCAGAACTGC 
CCAGAAATTG AAGCCCTGAT AAAGGAAAAC CATCGCTTCA AGAACATGGT CATTGTCACC 
ACCACCACGG AGTGCCTGAG GCATATCAGA CATGTTGGCG CCCTGACTGC GGAGGTGGGA 
GATATGACCG AAGACAGTGC CAAAGATCTC ATCGAGGCAG TGCTGGTACC TGATCAGGTT 
GAACGCCTGT GGGCCCAAAT CCAGGAGTCC AGGTGCCTGA GAAATCTGAT GAAGACCCCT 
CTCTTCGTGG TGATCACCTG TGCAATTCAG ATGGGCAGAC AGGAATTCCA AGCTCACACC 
CAAACCATGC TGTTCCAAAC CTTCTACGAC CTCCTGATAC AGAAAAACAG CCACAGATAT 
AGAGGTGGAG CTTCAGGTGA TTTTGCCAGG AGCCTAGACT ACTGTGGAGA CCTGGCCCTA 
GAAGGTGTGT TCGCCCACAA ATTTGATTTT GAACCCGAGC ATGGGTCCAG CATGAACGAG 
GACGTCCTGG TGACAATAGG GCTCCTCTGT AAGTACACAG CTCAGAGGCT GAAGCCCACG 
TATAAATTCT TTCATAAATC ATTTCAGGAG TACACGGCAG GTCGGAGACT CAGCAGTTTG 
CTGACGTCCA AAGAGCCAGA GGAGGTGAGC AAAGGGAACA GCTACTTAAA CAAAATGGTT 
TCCATCTCTG ACATCACATC CCTATATGGC AATCTGCTCC TCTACACGTG TGGGTCGTCC 
ACAGAAGCAA CCAGGGCGGT CATGAGGCAC CTTGCAATGG TTTATCAGCA CGGCAGCCTA 
CAAGGACTTT CAGTCACCAA GAGGCCTCTC TGGAGGCAGG AATCAATCCA GAGTCTGAGA 
AATACCACTG AGCAAGATGT TCTGAAAGCC ATCAATGTAA ATTCCTTCGT AGAGTGTGGC 
ATCAATTTGT TCTCAGAGAG TATGTCTAAA TCAGACCTGA GCCAAGAATT TGAAGCTTTC 
TTTCAAGGTA AAAGTTTATA CATCAACTCA GAGAACATCC CTGACTATTT ATTTGACTTC 
TTTGAATACT TGCCTAATTG TGCAAGCGCA TTGGACTTCG TGAAGTTGGA TTTCTATGAA 
AGAGCTACAG AGTCACAGGA CAAGGCAGAA GAGAATGTCC CTGGAGTTCA CACAGAAGGG 
CCCTCAGAAA CCTACATTCC CCCCAGGGCT GTGTCTTTGT TCTTCAACTG GAAGCAGGAA 
TTCAAGACTC TAGAGGTCAC ACTCCGAGAT ATTAACAAGT TGAATAAGCA AGATATCAAA 
TATCTGGGGA AGATATTCAG CTCTGCCACC AACCTCCGGC TGCATATCAA GAGATGTGCA 
GCCATGGCTG GAAGACTCAG CTCAGTCCTC AGAACCTGCA AGAACATGCA TACCCTCATG 
GTGGAAGCCA GTCCCCTCAC CACGGATGAC GAACAGTACA TCACATCTGT GACAGGCCTC 
CAGAACTTAA GTATTCACCG CTTGCACACT CAACAGCTGC CAGGTGGTCT GATTGACAGC 
TTGGGTAATC TGAAGAACCT CGAGAGACTC ATACTGGATG ACATCAGGAT GAACGAGGAA 
GATGCTAAAA ACCTAGCCGA AGGCCTACGA AGCCTGAAGA AGATGCGTTT ACTCCATTTG 
ACTCATTTGT CTGACATTGG GGAGGGGATG GACTACATAG TCAAGTCTCT CTCAGAAGAA 
TCCTGTGATC TCCAAGAGAT GAAGTTGGTG GCCTGCTGTC TGACTGCAAA CTCTGTGAAA 
GTTCTAGCAC AGAATCTTCA CAATTTGATC AAGCTGAGCA TTCTTGATAT ATCAGAAAAT 
TACCTGGAAA AGGATGGGAA TGAAGCTCTA CAGGAACTGA TCGGCAGGCT TGGCGTTCTG 
GGAGAGCTCA CTACATTGAT GCTGCCTTGG TGCTGGGATG TGCACACCAG CCTGCCCAAG 
CTGTTGAAGC AGTTGGAGGG GACCCCAGGA CTTGCCAAAC TTGGATTGAA AAACTGGAGA 
CTCAGAGACG AAGAGATTAA AAGTTTAGGT GAATTTCTGG AGATGAATCC TCTGAGAGAC 
TTGCAGCAGT TGGATTTAGC GGGGCACTGT GTGAGCAGTG ACGGATGGCT TTACTTCATG 
AATGTGTTTG AGAATCTGAA GCAGTTAGTG TTTTTTGACT TTAGCACTGA GGAGTTCTTA 
CCGGATGCAG CACTGGTGAG GAAACTTAGT CAAGTGTTAT CCAAGTTAAC TCTTCTGCAA 
GAGGTAAAGC TCACGGGCTG GGAGTTTGAT GACTATGATA TTAGCGCTAT TAAAGGCACC 
TTTAAACTAG TGACTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( XM_006524347.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524410.1 Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu53643
Clone ID Related Accession (Same CDS sequence)	XM_006524347.4 , XM_006524347.3
Accession Version	XM_006524347.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	NLR family CARD domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524347.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGA AGGACCATCG TCATCACCGT GTGGAGCAGC TGACTTTGGG CAGCCTGCTC 
GAGGCTCTGA AGAGCCCCTG CCTGATTGAA GGCGAGTCTG GCAAAGGGAA GTCCACCCTG 
CTGCAGAGAA TCGCTATGCT CTGGGCCTCT GGGGGCTGCA GGGCTCTGAA GGGGTTCAGA 
TTAGTCTTCT TCATCCACCT GAGAAGCGCC AGGGGGGGAC TATTCGAAAC ACTGTACGAT 
CAGCTCCTGA ACATACCCGA CTTCATCAGC AAGCCGACCT TCAAGGCTCT GCTGCTGAAG 
CTACACAAGG AGGTCCTCTT TCTTCTCGAT GGTTACAATG AATTCCATCC CCAGAACTGC 
CCAGAAATTG AAGCCCTGAT AAAGGAAAAC CATCGCTTCA AGAACATGGT CATTGTCACC 
ACCACCACGG AGTGCCTGAG GCATATCAGA CATGTTGGCG CCCTGACTGC GGAGGTGGGA 
GATATGACCG AAGACAGTGC CAAAGATCTC ATCGAGGCAG TGCTGGTACC TGATCAGGTT 
GAACGCCTGT GGGCCCAAAT CCAGGAGTCC AGGTGCCTGA GAAATCTGAT GAAGACCCCT 
CTCTTCGTGG TGATCACCTG TGCAATTCAG ATGGGCAGAC AGGAATTCCA AGCTCACACC 
CAAACCATGC TGTTCCAAAC CTTCTACGAC CTCCTGATAC AGAAAAACAG CCACAGATAT 
AGAGGTGGAG CTTCAGGTGA TTTTGCCAGG AGCCTAGACT ACTGTGGAGA CCTGGCCCTA 
GAAGGTGTGT TCGCCCACAA ATTTGATTTT GAACCCGAGC ATGGGTCCAG CATGAACGAG 
GACGTCCTGG TGACAATAGG GCTCCTCTGT AAGTACACAG CTCAGAGGCT GAAGCCCACG 
TATAAATTCT TTCATAAATC ATTTCAGGAG TACACGGCAG GTCGGAGACT CAGCAGTTTG 
CTGACGTCCA AAGAGCCAGA GGAGGTGAGC AAAGGGAACA GCTACTTAAA CAAAATGGTT 
TCCATCTCTG ACATCACATC CCTATATGGC AATCTGCTCC TCTACACGTG TGGGTCGTCC 
ACAGAAGCAA CCAGGGCGGT CATGAGGCAC CTTGCAATGG TTTATCAGCA CGGCAGCCTA 
CAAGGACTTT CAGTCACCAA GAGGCCTCTC TGGAGGCAGG AATCAATCCA GAGTCTGAGA 
AATACCACTG AGCAAGATGT TCTGAAAGCC ATCAATGTAA ATTCCTTCGT AGAGTGTGGC 
ATCAATTTGT TCTCAGAGAG TATGTCTAAA TCAGACCTGA GCCAAGAATT TGAAGCTTTC 
TTTCAAGGTA AAAGTTTATA CATCAACTCA GAGAACATCC CTGACTATTT ATTTGACTTC 
TTTGAATACT TGCCTAATTG TGCAAGCGCA TTGGACTTCG TGAAGTTGGA TTTCTATGAA 
AGAGCTACAG AGTCACAGGA CAAGGCAGAA GAGAATGTCC CTGGAGTTCA CACAGAAGGG 
CCCTCAGAAA CCTACATTCC CCCCAGGGCT GTGTCTTTGT TCTTCAACTG GAAGCAGGAA 
TTCAAGACTC TAGAGGTCAC ACTCCGAGAT ATTAACAAGT TGAATAAGCA AGATATCAAA 
TATCTGGGGA AGATATTCAG CTCTGCCACC AACCTCCGGC TGCATATCAA GAGATGTGCA 
GCCATGGCTG GAAGACTCAG CTCAGTCCTC AGAACCTGCA AGAACATGCA TACCCTCATG 
GTGGAAGCCA GTCCCCTCAC CACGGATGAC GAACAGTACA TCACATCTGT GACAGGCCTC 
CAGAACTTAA GTATTCACCG CTTGCACACT CAACAGCTGC CAGGTGGTCT GATTGACAGC 
TTGGGTAATC TGAAGAACCT CGAGAGACTC ATACTGGATG ACATCAGGAT GAACGAGGAA 
GATGCTAAAA ACCTAGCCGA AGGCCTACGA AGCCTGAAGA AGATGCGTTT ACTCCATTTG 
ACTCATTTGT CTGACATTGG GGAGGGGATG GACTACATAG TCAAGTCTCT CTCAGAAGAA 
TCCTGTGATC TCCAAGAGAT GAAGTTGGTG GCCTGCTGTC TGACTGCAAA CTCTGTGAAA 
GTTCTAGCAC AGAATCTTCA CAATTTGATC AAGCTGAGCA TTCTTGATAT ATCAGAAAAT 
TACCTGGAAA AGGATGGGAA TGAAGCTCTA CAGGAACTGA TCGGCAGGCT TGGCGTTCTG 
GGAGAGCTCA CTACATTGAT GCTGCCTTGG TGCTGGGATG TGCACACCAG CCTGCCCAAG 
CTGTTGAAGC AGTTGGAGGG GACCCCAGGA CTTGCCAAAC TTGGATTGAA AAACTGGAGA 
CTCAGAGACG AAGAGATTAA AAGTTTAGGT GAATTTCTGG AGATGAATCC TCTGAGAGAC 
TTGCAGCAGT TGGATTTAGC GGGGCACTGT GTGAGCAGTG ACGGATGGCT TTACTTCATG 
AATGTGTTTG AGAATCTGAA GCAGTTAGTG TTTTTTGACT TTAGCACTGA GGAGTTCTTA 
CCGGATGCAG CACTGGTGAG GAAACTTAGT CAAGTGTTAT CCAAGTTAAC TCTTCTGCAA 
GAGGTAAAGC TCACGGGCTG GGAGTTTGAT GACTATGATA TTAGCGCTAT TAAAGGCACC 
TTTAAACTAG TGACTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524410.1
CDS	652..3312
Translation	MWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA

Target ORF information:

RefSeq Version	XM_006524347.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524347.3

ORF Insert Sequence:

ATGTGGAAGA AGGACCATCG TCATCACCGT GTGGAGCAGC TGACTTTGGG CAGCCTGCTC 
GAGGCTCTGA AGAGCCCCTG CCTGATTGAA GGCGAGTCTG GCAAAGGGAA GTCCACCCTG 
CTGCAGAGAA TCGCTATGCT CTGGGCCTCT GGGGGCTGCA GGGCTCTGAA GGGGTTCAGA 
TTAGTCTTCT TCATCCACCT GAGAAGCGCC AGGGGGGGAC TATTCGAAAC ACTGTACGAT 
CAGCTCCTGA ACATACCCGA CTTCATCAGC AAGCCGACCT TCAAGGCTCT GCTGCTGAAG 
CTACACAAGG AGGTCCTCTT TCTTCTCGAT GGTTACAATG AATTCCATCC CCAGAACTGC 
CCAGAAATTG AAGCCCTGAT AAAGGAAAAC CATCGCTTCA AGAACATGGT CATTGTCACC 
ACCACCACGG AGTGCCTGAG GCATATCAGA CATGTTGGCG CCCTGACTGC GGAGGTGGGA 
GATATGACCG AAGACAGTGC CAAAGATCTC ATCGAGGCAG TGCTGGTACC TGATCAGGTT 
GAACGCCTGT GGGCCCAAAT CCAGGAGTCC AGGTGCCTGA GAAATCTGAT GAAGACCCCT 
CTCTTCGTGG TGATCACCTG TGCAATTCAG ATGGGCAGAC AGGAATTCCA AGCTCACACC 
CAAACCATGC TGTTCCAAAC CTTCTACGAC CTCCTGATAC AGAAAAACAG CCACAGATAT 
AGAGGTGGAG CTTCAGGTGA TTTTGCCAGG AGCCTAGACT ACTGTGGAGA CCTGGCCCTA 
GAAGGTGTGT TCGCCCACAA ATTTGATTTT GAACCCGAGC ATGGGTCCAG CATGAACGAG 
GACGTCCTGG TGACAATAGG GCTCCTCTGT AAGTACACAG CTCAGAGGCT GAAGCCCACG 
TATAAATTCT TTCATAAATC ATTTCAGGAG TACACGGCAG GTCGGAGACT CAGCAGTTTG 
CTGACGTCCA AAGAGCCAGA GGAGGTGAGC AAAGGGAACA GCTACTTAAA CAAAATGGTT 
TCCATCTCTG ACATCACATC CCTATATGGC AATCTGCTCC TCTACACGTG TGGGTCGTCC 
ACAGAAGCAA CCAGGGCGGT CATGAGGCAC CTTGCAATGG TTTATCAGCA CGGCAGCCTA 
CAAGGACTTT CAGTCACCAA GAGGCCTCTC TGGAGGCAGG AATCAATCCA GAGTCTGAGA 
AATACCACTG AGCAAGATGT TCTGAAAGCC ATCAATGTAA ATTCCTTCGT AGAGTGTGGC 
ATCAATTTGT TCTCAGAGAG TATGTCTAAA TCAGACCTGA GCCAAGAATT TGAAGCTTTC 
TTTCAAGGTA AAAGTTTATA CATCAACTCA GAGAACATCC CTGACTATTT ATTTGACTTC 
TTTGAATACT TGCCTAATTG TGCAAGCGCA TTGGACTTCG TGAAGTTGGA TTTCTATGAA 
AGAGCTACAG AGTCACAGGA CAAGGCAGAA GAGAATGTCC CTGGAGTTCA CACAGAAGGG 
CCCTCAGAAA CCTACATTCC CCCCAGGGCT GTGTCTTTGT TCTTCAACTG GAAGCAGGAA 
TTCAAGACTC TAGAGGTCAC ACTCCGAGAT ATTAACAAGT TGAATAAGCA AGATATCAAA 
TATCTGGGGA AGATATTCAG CTCTGCCACC AACCTCCGGC TGCATATCAA GAGATGTGCA 
GCCATGGCTG GAAGACTCAG CTCAGTCCTC AGAACCTGCA AGAACATGCA TACCCTCATG 
GTGGAAGCCA GTCCCCTCAC CACGGATGAC GAACAGTACA TCACATCTGT GACAGGCCTC 
CAGAACTTAA GTATTCACCG CTTGCACACT CAACAGCTGC CAGGTGGTCT GATTGACAGC 
TTGGGTAATC TGAAGAACCT CGAGAGACTC ATACTGGATG ACATCAGGAT GAACGAGGAA 
GATGCTAAAA ACCTAGCCGA AGGCCTACGA AGCCTGAAGA AGATGCGTTT ACTCCATTTG 
ACTCATTTGT CTGACATTGG GGAGGGGATG GACTACATAG TCAAGTCTCT CTCAGAAGAA 
TCCTGTGATC TCCAAGAGAT GAAGTTGGTG GCCTGCTGTC TGACTGCAAA CTCTGTGAAA 
GTTCTAGCAC AGAATCTTCA CAATTTGATC AAGCTGAGCA TTCTTGATAT ATCAGAAAAT 
TACCTGGAAA AGGATGGGAA TGAAGCTCTA CAGGAACTGA TCGGCAGGCT TGGCGTTCTG 
GGAGAGCTCA CTACATTGAT GCTGCCTTGG TGCTGGGATG TGCACACCAG CCTGCCCAAG 
CTGTTGAAGC AGTTGGAGGG GACCCCAGGA CTTGCCAAAC TTGGATTGAA AAACTGGAGA 
CTCAGAGACG AAGAGATTAA AAGTTTAGGT GAATTTCTGG AGATGAATCC TCTGAGAGAC 
TTGCAGCAGT TGGATTTAGC GGGGCACTGT GTGAGCAGTG ACGGATGGCT TTACTTCATG 
AATGTGTTTG AGAATCTGAA GCAGTTAGTG TTTTTTGACT TTAGCACTGA GGAGTTCTTA 
CCGGATGCAG CACTGGTGAG GAAACTTAGT CAAGTGTTAT CCAAGTTAAC TCTTCTGCAA 
GAGGTAAAGC TCACGGGCTG GGAGTTTGAT GACTATGATA TTAGCGCTAT TAAAGGCACC 
TTTAAACTAG TGACTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( NM_001033367.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028539.1 Mus musculus NLR family, CARD domain containing 4 (Nlrc4), mRNA.

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CloneID	OMu19575
Clone ID Related Accession (Same CDS sequence)	XM_006524346.3 , XM_006524346.4 , XM_017317490.1 , XM_017317491.1 , NM_001033367.3
Accession Version	NM_001033367.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	NLR family CARD domain-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT033749.8. On May 2, 2007 this sequence version replaced NM_001033367.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143864.1, AK171624.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028539.1
CDS	217..3291
Translation	MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFLKSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA

Target ORF information:

RefSeq Version	NM_001033367.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033367.3

ORF Insert Sequence:

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( XM_006524346.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524409.1 Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu19575
Clone ID Related Accession (Same CDS sequence)	XM_006524346.3 , XM_006524346.4 , XM_017317490.1 , XM_017317491.1 , NM_001033367.3
Accession Version	XM_006524346.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	NLR family CARD domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524409.1
CDS	344..3418
Translation	MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFLKSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA

Target ORF information:

RefSeq Version	XM_006524346.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524346.3

ORF Insert Sequence:

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( XM_006524346.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524409.1 Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu19575
Clone ID Related Accession (Same CDS sequence)	XM_006524346.3 , XM_006524346.4 , XM_017317490.1 , XM_017317491.1 , NM_001033367.3
Accession Version	XM_006524346.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	NLR family CARD domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524346.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524409.1
CDS	343..3417
Translation	MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFLKSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA

Target ORF information:

RefSeq Version	XM_006524346.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524346.4

ORF Insert Sequence:

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( XM_017317490.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172979.1 Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19575
Clone ID Related Accession (Same CDS sequence)	XM_006524346.3 , XM_006524346.4 , XM_017317490.1 , XM_017317491.1 , NM_001033367.3
Accession Version	XM_017317490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	NLR family CARD domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172979.1
CDS	849..3923
Translation	MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFLKSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA

Target ORF information:

RefSeq Version	XM_017317490.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317490.1

ORF Insert Sequence:

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrc4 ( XM_017317491.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172980.1 Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X3, mRNA.

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CloneID	OMu19575
Clone ID Related Accession (Same CDS sequence)	XM_006524346.3 , XM_006524346.4 , XM_017317490.1 , XM_017317491.1 , NM_001033367.3
Accession Version	XM_017317491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	NLR family CARD domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172980.1
CDS	242..3316
Translation	MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFLKSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARGGLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMGRQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDVLVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNLLLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGINLFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEENVPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAMAGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLILDDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVLAQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLAKLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPDAALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA

Target ORF information:

RefSeq Version	XM_017317491.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, CARD domain containing 4 (Nlrc4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317491.1

ORF Insert Sequence:

ATGAACTTTA TAAGGAACAA CAGACGAGCC CTTATTCAAA GGATGGGCTT AACAGTTACC 
AAGCAAATCT GCGATGACCT CTTTGCATTG AACGTTCTCA ACAATCAAGA AGCTAATGTC 
ATTTACTGTG AGCCCTTGGA GCAGGAAGCC GCCCGAAAGA TCATCCATAT GACTATGCAG 
AAGGGCTCAG CGGCCTGCAA CCTCTTTCTT AAGAGTCTTG AAAACTGGGA CTATTTTGTG 
TATCAGGACT TAACTGGACA AAATCTTTCT TATCAGGTCA CAGAAGAAGA CCTGAATGTT 
TTGGCCCAGA ATTTAAAGGA CTTGTACAAC AGCCCTGCTT TTCTGAACTT CTACCCCCTG 
GGTGAAGATA TCGACATAAT TTTTAATCTG GAGAAAACCT TCACAGAACC TATCATGTGG 
AAGAAGGACC ATCGTCATCA CCGTGTGGAG CAGCTGACTT TGGGCAGCCT GCTCGAGGCT 
CTGAAGAGCC CCTGCCTGAT TGAAGGCGAG TCTGGCAAAG GGAAGTCCAC CCTGCTGCAG 
AGAATCGCTA TGCTCTGGGC CTCTGGGGGC TGCAGGGCTC TGAAGGGGTT CAGATTAGTC 
TTCTTCATCC ACCTGAGAAG CGCCAGGGGG GGACTATTCG AAACACTGTA CGATCAGCTC 
CTGAACATAC CCGACTTCAT CAGCAAGCCG ACCTTCAAGG CTCTGCTGCT GAAGCTACAC 
AAGGAGGTCC TCTTTCTTCT CGATGGTTAC AATGAATTCC ATCCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCATCGC TTCAAGAACA TGGTCATTGT CACCACCACC 
ACGGAGTGCC TGAGGCATAT CAGACATGTT GGCGCCCTGA CTGCGGAGGT GGGAGATATG 
ACCGAAGACA GTGCCAAAGA TCTCATCGAG GCAGTGCTGG TACCTGATCA GGTTGAACGC 
CTGTGGGCCC AAATCCAGGA GTCCAGGTGC CTGAGAAATC TGATGAAGAC CCCTCTCTTC 
GTGGTGATCA CCTGTGCAAT TCAGATGGGC AGACAGGAAT TCCAAGCTCA CACCCAAACC 
ATGCTGTTCC AAACCTTCTA CGACCTCCTG ATACAGAAAA ACAGCCACAG ATATAGAGGT 
GGAGCTTCAG GTGATTTTGC CAGGAGCCTA GACTACTGTG GAGACCTGGC CCTAGAAGGT 
GTGTTCGCCC ACAAATTTGA TTTTGAACCC GAGCATGGGT CCAGCATGAA CGAGGACGTC 
CTGGTGACAA TAGGGCTCCT CTGTAAGTAC ACAGCTCAGA GGCTGAAGCC CACGTATAAA 
TTCTTTCATA AATCATTTCA GGAGTACACG GCAGGTCGGA GACTCAGCAG TTTGCTGACG 
TCCAAAGAGC CAGAGGAGGT GAGCAAAGGG AACAGCTACT TAAACAAAAT GGTTTCCATC 
TCTGACATCA CATCCCTATA TGGCAATCTG CTCCTCTACA CGTGTGGGTC GTCCACAGAA 
GCAACCAGGG CGGTCATGAG GCACCTTGCA ATGGTTTATC AGCACGGCAG CCTACAAGGA 
CTTTCAGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCAA TCCAGAGTCT GAGAAATACC 
ACTGAGCAAG ATGTTCTGAA AGCCATCAAT GTAAATTCCT TCGTAGAGTG TGGCATCAAT 
TTGTTCTCAG AGAGTATGTC TAAATCAGAC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGTT TATACATCAA CTCAGAGAAC ATCCCTGACT ATTTATTTGA CTTCTTTGAA 
TACTTGCCTA ATTGTGCAAG CGCATTGGAC TTCGTGAAGT TGGATTTCTA TGAAAGAGCT 
ACAGAGTCAC AGGACAAGGC AGAAGAGAAT GTCCCTGGAG TTCACACAGA AGGGCCCTCA 
GAAACCTACA TTCCCCCCAG GGCTGTGTCT TTGTTCTTCA ACTGGAAGCA GGAATTCAAG 
ACTCTAGAGG TCACACTCCG AGATATTAAC AAGTTGAATA AGCAAGATAT CAAATATCTG 
GGGAAGATAT TCAGCTCTGC CACCAACCTC CGGCTGCATA TCAAGAGATG TGCAGCCATG 
GCTGGAAGAC TCAGCTCAGT CCTCAGAACC TGCAAGAACA TGCATACCCT CATGGTGGAA 
GCCAGTCCCC TCACCACGGA TGACGAACAG TACATCACAT CTGTGACAGG CCTCCAGAAC 
TTAAGTATTC ACCGCTTGCA CACTCAACAG CTGCCAGGTG GTCTGATTGA CAGCTTGGGT 
AATCTGAAGA ACCTCGAGAG ACTCATACTG GATGACATCA GGATGAACGA GGAAGATGCT 
AAAAACCTAG CCGAAGGCCT ACGAAGCCTG AAGAAGATGC GTTTACTCCA TTTGACTCAT 
TTGTCTGACA TTGGGGAGGG GATGGACTAC ATAGTCAAGT CTCTCTCAGA AGAATCCTGT 
GATCTCCAAG AGATGAAGTT GGTGGCCTGC TGTCTGACTG CAAACTCTGT GAAAGTTCTA 
GCACAGAATC TTCACAATTT GATCAAGCTG AGCATTCTTG ATATATCAGA AAATTACCTG 
GAAAAGGATG GGAATGAAGC TCTACAGGAA CTGATCGGCA GGCTTGGCGT TCTGGGAGAG 
CTCACTACAT TGATGCTGCC TTGGTGCTGG GATGTGCACA CCAGCCTGCC CAAGCTGTTG 
AAGCAGTTGG AGGGGACCCC AGGACTTGCC AAACTTGGAT TGAAAAACTG GAGACTCAGA 
GACGAAGAGA TTAAAAGTTT AGGTGAATTT CTGGAGATGA ATCCTCTGAG AGACTTGCAG 
CAGTTGGATT TAGCGGGGCA CTGTGTGAGC AGTGACGGAT GGCTTTACTT CATGAATGTG 
TTTGAGAATC TGAAGCAGTT AGTGTTTTTT GACTTTAGCA CTGAGGAGTT CTTACCGGAT 
GCAGCACTGG TGAGGAAACT TAGTCAAGTG TTATCCAAGT TAACTCTTCT GCAAGAGGTA 
AAGCTCACGG GCTGGGAGTT TGATGACTAT GATATTAGCG CTATTAAAGG CACCTTTAAA 
CTAGTGACTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Nlrp6 inflammasome is not required for baseline colonic inner mucus layer formation or function.
The Journal of experimental medicine216(11)2602-2618(2019 Nov)
Volk JK,Nyström EEL,van der Post S,Abad BM,Schroeder BO,Johansson Å,Svensson F,Jäverfelt S,Johansson MEV,Hansson GC,Birchenough GMH

NLRC4 suppresses IL-17A-mediated neutrophil-dependent host defense through upregulation of IL-18 and induction of necroptosis during Gram-positive pneumonia.
Mucosal immunology12(1)247-257(2019 01)
Paudel S,Ghimire L,Jin L,Baral P,Cai S,Jeyaseelan S

Autoinflammatory mutation in NLRC4 reveals a leucine-rich repeat (LRR)-LRR oligomerization interface.
The Journal of allergy and clinical immunology142(6)1956-1967.e6(2018 12)
Moghaddas F,Zeng P,Zhang Y,Schützle H,Brenner S,Hofmann SR,Berner R,Zhao Y,Lu B,Chen X,Zhang L,Cheng S,Winkler S,Lehmberg K,Canna SW,Czabotar PE,Wicks IP,De Nardo D,Hedrich CM,Zeng H,Masters SL

IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.
Cell173(4)920-933.e13(2018 05)
Karki R,Lee E,Place D,Samir P,Mavuluri J,Sharma BR,Balakrishnan A,Malireddi RKS,Geiger R,Zhu Q,Neale G,Kanneganti TD

ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Scientific reports8(1)3788(2018 02)
Lee BL,Mirrashidi KM,Stowe IB,Kummerfeld SK,Watanabe C,Haley B,Cuellar TL,Reichelt M,Kayagaki N

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Nlrc4 cDNA ORF clone, Mus musculus(house mouse)

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