NLRC4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRC4
Entrez Gene ID	512480
Full Name	NLR family CARD domain containing 4
General protein information	Preferred Names NLR family CARD domain containing 4 Names NLR family CARD domain-containing protein 4 ice protease-activating factor ipaf
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192323.2	NP_001179252.2	NLR family CARD domain-containing protein 4
XM_010809867.2	XP_010808169.1	NLR family CARD domain-containing protein 4 isoform X2
XM_010809868.2	XP_010808170.1	NLR family CARD domain-containing protein 4 isoform X3
XM_005212521.3	XP_005212578.1	NLR family CARD domain-containing protein 4 isoform X1
XM_010809868.3	XP_010808170.2	NLR family CARD domain-containing protein 4 isoform X2
XM_005212521.4	XP_005212578.2	NLR family CARD domain-containing protein 4 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NLRC4	XP_001105818.2
Bos taurus (cattle)	NLRC4	NP_001179252.2
Homo sapiens (human)	NLRC4	NP_001186067.1
Pan troglodytes (chimpanzee)	NLRC4	XP_515390.2
Xenopus tropicalis (tropical clawed frog)	nlrc4	XP_004914732.1
Mus musculus (house mouse)	Nlrc4	NP_001028539.1
Rattus norvegicus (Norway rat)	Nlrc4	XP_003750157.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NLRC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220267	XM_010809868.3 Latest version!	Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa48320	XM_010809867.2 Latest version!	Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa48321	XM_010809868.2 Latest version!	Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa01327	NM_001192323.2 Latest version!	Bos taurus NLR family CARD domain containing 4 (NLRC4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa01327	XM_005212521.3 Latest version!	Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220266	XM_005212521.4 Latest version!	Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRC4 ( XM_010809868.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808170.2 Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220267
Clone ID Related Accession (Same CDS sequence)	XM_010809868.3
Accession Version	XM_010809868.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	NLR family CARD domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809868.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA 
TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG 
CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT 
CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA 
AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT 
CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT 
GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA 
GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT 
TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT 
AAAGGTGCTT TTAAACTAGT AATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808170.2
CDS	398..3187
Translation	MNFIKENSQVLIQRMGMTVIKQILDELFVWNVMNYEEVNVICGEKFEQDAARGVIHMILKKGSEACNLFLKSLEKWNYPLFQELHGLSLFHQMSEEDLDDLAQELKYFYQSPSFLNFYPLGEDIDIMFNLKSTFTEPVLWKKDQHHHRLEQLTLSGLLDTLQSPCIIEGESGKGKSTLLQRIAMLWASGECQALTKFKLVFFLRLSRAQGGLFETLSDQLLDIPDVISKQTFMARLLKLRQRVLFLLDGYNEFKAQNCPEIEALIKENHRFKNMVIVTTTTESLRHIRQFGALIAEVGDMTESSAQALIQEVLRKEFAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMGKSEFHSHTQTTLFCTFYDLLINKNRHKRKGLAPSEVTQSLDHCGDLALEGVFSRRFDFEPDDLSNVNEDVLLTTGLLCKYTAQRFKPKYKFFHQSFQEYTAGRRLSSLLTSGEPAEVTKGNGHLQKMVSISDITSKYSNLLLYTCGSSAEATRTVLKHLSSVYQHGSLLGLSVTKRPLWRQESMQNMKSTTVQEILKAININSFTECGINLFHESISTSSLSKEFEDFFRGKSLYINSENIPDYLFDFFEDLPNCASALDFVKLDFYGGAVRDISGNQDQEFSGTYIPSRAVSLFFNWKQEFKTLDVTLRDFCKLSKKDIKYLEKIFSSATSLRLHIKRCVGMAGSLSSVLSTCKNIHSLIVEASPLTLEDEQHITSVTNLQTLGVHDLQIQRLPAQNLHNLARLSILDLSENHLEKDGKEALQQLIDRLHILEQLTVLMLPWCGDVRVSLARLLEQLERVPQLVKLGLKNWRLTDAEIRILGVFFEKNPLENFQQLDLAGNCVSSDGWLAFMSGFENLKELVFFDFSTKGLLPDASLVRKLSHVLSKLTFLQEVQLVGWQLDDDDVSVLKGAFKLVIA

Target ORF information:

RefSeq Version	XM_010809868.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809868.3

ORF Insert Sequence:

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA 
TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG 
CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT 
CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA 
AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT 
CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT 
GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA 
GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT 
TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT 
AAAGGTGCTT TTAAACTAGT AATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC4 ( XM_010809867.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808169.1 Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa48320
Clone ID Related Accession (Same CDS sequence)	XM_010809867.2
Accession Version	XM_010809867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	NLR family CARD domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TCAGAATCTT 
CATAACCTGG CCAGACTGAG CATTCTTGAT TTATCTGAAA ATCATCTGGA AAAGGATGGA 
AAAGAAGCTC TTCAGCAACT AATTGACAGG CTGCATATCT TAGAACAGCT AACCGTTCTG 
ATGCTGCCCT GGTGTGGTGA TGTACGAGTC AGTCTGGCCA GACTGTTGGA GCAACTGGAG 
AGGGTCCCAC AGCTCGTCAA GCTTGGGCTG AAAAACTGGA GACTCACAGA TGCAGAGATA 
AGAATTCTAG GTGTATTTTT TGAAAAGAAT CCTCTGGAAA ACTTCCAGCA GTTGGATTTG 
GCAGGAAATT GTGTAAGCAG CGATGGATGG CTTGCCTTCA TGAGTGGATT TGAGAATCTT 
AAGGAACTAG TGTTTTTTGA CTTCAGTACG AAAGGACTCT TACCTGATGC ATCATTAGTC 
AGGAAACTTA GCCACGTGTT ATCCAAACTA ACTTTTCTGC AAGAGGTTCA GCTTGTTGGG 
TGGCAACTTG ATGACGATGA TGTCAGTGTT CTTAAAGGTG CTTTTAAACT AGTAATTGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808169.1
CDS	517..3399
Translation	MNFIKENSQVLIQRMGMTVIKQILDELFVWNVMNYEEVNVICGEKFEQDAARGVIHMILKKGSEACNLFLKSLEKWNYPLFQELHGLSLFHQMSEEDLDDLAQELKYFYQSPSFLNFYPLGEDIDIMFNLKSTFTEPVLWKKDQHHHRLEQLTLSGLLDTLQSPCIIEGESGKGKSTLLQRIAMLWASGECQALTKFKLVFFLRLSRAQGGLFETLSDQLLDIPDVISKQTFMARLLKLRQRVLFLLDGYNEFKAQNCPEIEALIKENHRFKNMVIVTTTTESLRHIRQFGALIAEVGDMTESSAQALIQEVLRKEFAEDLLLQIQKSRCLRNLMKTPLFVVITCAIQMGKSEFHSHTQTTLFCTFYDLLINKNRHKRKGLAPSEVTQSLDHCGDLALEGVFSRRFDFEPDDLSNVNEDVLLTTGLLCKYTAQRFKPKYKFFHQSFQEYTAGRRLSSLLTSGEPAEVTKGNGHLQKMVSISDITSKYSNLLLYTCGSSAEATRTVLKHLSSVYQHGSLLGLSVTKRPLWRQESMQNMKSTTVQEILKAININSFTECGINLFHESISTSSLSKEFEDFFRGKSLYINSENIPDYLFDFFEDLPNCASALDFVKLDFYGGAVRDISGNQDQEFSGTYIPSRAVSLFFNWKQEFKTLDVTLRDFCKLSKKDIKYLEKIFSSATSLRLHIKRCVGMAGSLSSVLSTCKNIHSLIVEASPLTLEDEQHITSVTNLQTLGVHDLQIQRLPGGLTDNLGNLKNLMKLILDNIQMNEEDALKLAQNLHNLARLSILDLSENHLEKDGKEALQQLIDRLHILEQLTVLMLPWCGDVRVSLARLLEQLERVPQLVKLGLKNWRLTDAEIRILGVFFEKNPLENFQQLDLAGNCVSSDGWLAFMSGFENLKELVFFDFSTKGLLPDASLVRKLSHVLSKLTFLQEVQLVGWQLDDDDVSVLKGAFKLVIA

Target ORF information:

RefSeq Version	XM_010809867.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809867.2

ORF Insert Sequence:

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TCAGAATCTT 
CATAACCTGG CCAGACTGAG CATTCTTGAT TTATCTGAAA ATCATCTGGA AAAGGATGGA 
AAAGAAGCTC TTCAGCAACT AATTGACAGG CTGCATATCT TAGAACAGCT AACCGTTCTG 
ATGCTGCCCT GGTGTGGTGA TGTACGAGTC AGTCTGGCCA GACTGTTGGA GCAACTGGAG 
AGGGTCCCAC AGCTCGTCAA GCTTGGGCTG AAAAACTGGA GACTCACAGA TGCAGAGATA 
AGAATTCTAG GTGTATTTTT TGAAAAGAAT CCTCTGGAAA ACTTCCAGCA GTTGGATTTG 
GCAGGAAATT GTGTAAGCAG CGATGGATGG CTTGCCTTCA TGAGTGGATT TGAGAATCTT 
AAGGAACTAG TGTTTTTTGA CTTCAGTACG AAAGGACTCT TACCTGATGC ATCATTAGTC 
AGGAAACTTA GCCACGTGTT ATCCAAACTA ACTTTTCTGC AAGAGGTTCA GCTTGTTGGG 
TGGCAACTTG ATGACGATGA TGTCAGTGTT CTTAAAGGTG CTTTTAAACT AGTAATTGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC4 ( XM_010809868.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808170.1 Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X3, mRNA.

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CloneID	OBa48321
Clone ID Related Accession (Same CDS sequence)	XM_010809868.2
Accession Version	XM_010809868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	NLR family CARD domain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA 
TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG 
CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT 
CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA 
AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT 
CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT 
GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA 
GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT 
TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT 
AAAGGTGCTT TTAAACTAGT AATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808170.1
CDS	517..3306
Translation	MNFIKENSQVLIQRMGMTVIKQILDELFVWNVMNYEEVNVICGEKFEQDAARGVIHMILKKGSEACNLFLKSLEKWNYPLFQELHGLSLFHQMSEEDLDDLAQELKYFYQSPSFLNFYPLGEDIDIMFNLKSTFTEPVLWKKDQHHHRLEQLTLSGLLDTLQSPCIIEGESGKGKSTLLQRIAMLWASGECQALTKFKLVFFLRLSRAQGGLFETLSDQLLDIPDVISKQTFMARLLKLRQRVLFLLDGYNEFKAQNCPEIEALIKENHRFKNMVIVTTTTESLRHIRQFGALIAEVGDMTESSAQALIQEVLRKEFAEDLLLQIQKSRCLRNLMKTPLFVVITCAIQMGKSEFHSHTQTTLFCTFYDLLINKNRHKRKGLAPSEVTQSLDHCGDLALEGVFSRRFDFEPDDLSNVNEDVLLTTGLLCKYTAQRFKPKYKFFHQSFQEYTAGRRLSSLLTSGEPAEVTKGNGHLQKMVSISDITSKYSNLLLYTCGSSAEATRTVLKHLSSVYQHGSLLGLSVTKRPLWRQESMQNMKSTTVQEILKAININSFTECGINLFHESISTSSLSKEFEDFFRGKSLYINSENIPDYLFDFFEDLPNCASALDFVKLDFYGGAVRDISGNQDQEFSGTYIPSRAVSLFFNWKQEFKTLDVTLRDFCKLSKKDIKYLEKIFSSATSLRLHIKRCVGMAGSLSSVLSTCKNIHSLIVEASPLTLEDEQHITSVTNLQTLGVHDLQIQRLPAQNLHNLARLSILDLSENHLEKDGKEALQQLIDRLHILEQLTVLMLPWCGDVRVSLARLLEQLERVPQLVKLGLKNWRLTDAEIRILGVFFEKNPLENFQQLDLAGNCVSSDGWLAFMSGFENLKELVFFDFSTKGLLPDASLVRKLSHVLSKLTFLQEVQLVGWQLDDDDVSVLKGAFKLVIA

Target ORF information:

RefSeq Version	XM_010809868.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809868.2

ORF Insert Sequence:

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGCTCA GAATCTTCAT AACCTGGCCA GACTGAGCAT TCTTGATTTA 
TCTGAAAATC ATCTGGAAAA GGATGGAAAA GAAGCTCTTC AGCAACTAAT TGACAGGCTG 
CATATCTTAG AACAGCTAAC CGTTCTGATG CTGCCCTGGT GTGGTGATGT ACGAGTCAGT 
CTGGCCAGAC TGTTGGAGCA ACTGGAGAGG GTCCCACAGC TCGTCAAGCT TGGGCTGAAA 
AACTGGAGAC TCACAGATGC AGAGATAAGA ATTCTAGGTG TATTTTTTGA AAAGAATCCT 
CTGGAAAACT TCCAGCAGTT GGATTTGGCA GGAAATTGTG TAAGCAGCGA TGGATGGCTT 
GCCTTCATGA GTGGATTTGA GAATCTTAAG GAACTAGTGT TTTTTGACTT CAGTACGAAA 
GGACTCTTAC CTGATGCATC ATTAGTCAGG AAACTTAGCC ACGTGTTATC CAAACTAACT 
TTTCTGCAAG AGGTTCAGCT TGTTGGGTGG CAACTTGATG ACGATGATGT CAGTGTTCTT 
AAAGGTGCTT TTAAACTAGT AATTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC4 ( NM_001192323.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179252.2 Bos taurus NLR family CARD domain containing 4 (NLRC4), mRNA.

Price & Availability↑

CloneID	OBa01327
Clone ID Related Accession (Same CDS sequence)	NM_001192323.2 , XM_005212521.3
Accession Version	NM_001192323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	NLR family CARD domain-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02030442.1. On Apr 15, 2011 this sequence version replaced NM_001192323.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG 
ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA 
ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA 
GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG 
GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT 
CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC 
TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA 
CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA 
GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT 
TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA 
GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT 
AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT 
GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179252.2
CDS	20..3073
Translation	MNFIKENSQVLIQRMGMTVIKQILDELFVWNVMNYEEVNVICGEKFEQDAARGVIHMILKKGSEACNLFLKSLEKWNYPLFQELHGLSLFHQMSEEDLDDLAQELKYFYQSPSFLNFYPLGEDIDIMFNLKSTFTEPVLWKKDQHHHRLEQLTLSGLLDTLQSPCIIEGESGKGKSTLLQRIAMLWASGECQALTKFKLVFFLRLSRAQGGLFETLSDQLLDIPDVISKQTFMARLLKLRQRVLFLLDGYNEFKAQNCPEIEALIKENHRFKNMVIVTTTTESLRHIRQFGALIAEVGDMTESSAQALIQEVLRKEFAEDLLLQIQKSRCLRNLMKTPLFVVITCAIQMGKSEFHSHTQTTLFCTFYDLLINKNRHKRKGLAPSEVTQSLDHCGDLALEGVFSRRFDFEPDDLSNVNEDVLLTTGLLCKYTAQRFKPKYKFFHQSFQEYTAGRRLSSLLTSGEPAEVTKGNGHLQKMVSISDITSKYSNLLLYTCGSSAEATRTVLKHLSSVYQHGSLLGLSVTKRPLWRQESMQNMKSTTVQEILKAININSFTECGINLFHESISTSSLSKEFEDFFRGKSLYINSENIPDYLFDFFEDLPNCASALDFVKLDFYGGAVRDISGNQDQEFSGTYIPSRAVSLFFNWKQEFKTLDVTLRDFCKLSKKDIKYLEKIFSSATSLRLHIKRCVGMAGSLSSVLSTCKNIHSLIVEASPLTLEDEQHITSVTNLQTLGVHDLQIQRLPGGLTDNLGNLKNLMKLILDNIQMNEEDALKLAEGLTNLKKMCLLRLTHLSDIGEGMDYIVKSLSAEPCDLKEIQLVSCCLSGNAVKTLAQNLHNLARLSILDLSENHLEKDGKEALQQLIDRLHILEQLTVLMLPWCGDVRVSLARLLEQLERVPQLVKLGLKNWRLTDAEIRILGVFFEKNPLENFQQLDLAGNCVSSDGWLAFMSGFENLKELVFFDFSTKGLLPDASLVRKLSHVLSKLTFLQEVQLVGWQLDDDDVSVLKGAFKLVIA

Target ORF information:

RefSeq Version	NM_001192323.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family CARD domain containing 4 (NLRC4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192323.2

ORF Insert Sequence:

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG 
ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA 
ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA 
GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG 
GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT 
CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC 
TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA 
CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA 
GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT 
TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA 
GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT 
AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT 
GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC4 ( XM_005212521.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212578.1 Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa01327
Clone ID Related Accession (Same CDS sequence)	NM_001192323.2 , XM_005212521.3
Accession Version	XM_005212521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	NLR family CARD domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104291.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG 
ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA 
ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA 
GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG 
GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT 
CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC 
TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA 
CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA 
GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT 
TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA 
GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT 
AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT 
GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212578.1
CDS	507..3560
Translation	MNFIKENSQVLIQRMGMTVIKQILDELFVWNVMNYEEVNVICGEKFEQDAARGVIHMILKKGSEACNLFLKSLEKWNYPLFQELHGLSLFHQMSEEDLDDLAQELKYFYQSPSFLNFYPLGEDIDIMFNLKSTFTEPVLWKKDQHHHRLEQLTLSGLLDTLQSPCIIEGESGKGKSTLLQRIAMLWASGECQALTKFKLVFFLRLSRAQGGLFETLSDQLLDIPDVISKQTFMARLLKLRQRVLFLLDGYNEFKAQNCPEIEALIKENHRFKNMVIVTTTTESLRHIRQFGALIAEVGDMTESSAQALIQEVLRKEFAEDLLLQIQKSRCLRNLMKTPLFVVITCAIQMGKSEFHSHTQTTLFCTFYDLLINKNRHKRKGLAPSEVTQSLDHCGDLALEGVFSRRFDFEPDDLSNVNEDVLLTTGLLCKYTAQRFKPKYKFFHQSFQEYTAGRRLSSLLTSGEPAEVTKGNGHLQKMVSISDITSKYSNLLLYTCGSSAEATRTVLKHLSSVYQHGSLLGLSVTKRPLWRQESMQNMKSTTVQEILKAININSFTECGINLFHESISTSSLSKEFEDFFRGKSLYINSENIPDYLFDFFEDLPNCASALDFVKLDFYGGAVRDISGNQDQEFSGTYIPSRAVSLFFNWKQEFKTLDVTLRDFCKLSKKDIKYLEKIFSSATSLRLHIKRCVGMAGSLSSVLSTCKNIHSLIVEASPLTLEDEQHITSVTNLQTLGVHDLQIQRLPGGLTDNLGNLKNLMKLILDNIQMNEEDALKLAEGLTNLKKMCLLRLTHLSDIGEGMDYIVKSLSAEPCDLKEIQLVSCCLSGNAVKTLAQNLHNLARLSILDLSENHLEKDGKEALQQLIDRLHILEQLTVLMLPWCGDVRVSLARLLEQLERVPQLVKLGLKNWRLTDAEIRILGVFFEKNPLENFQQLDLAGNCVSSDGWLAFMSGFENLKELVFFDFSTKGLLPDASLVRKLSHVLSKLTFLQEVQLVGWQLDDDDVSVLKGAFKLVIA

Target ORF information:

RefSeq Version	XM_005212521.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family, CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212521.3

ORF Insert Sequence:

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGAC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG 
ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA 
ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA 
GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG 
GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT 
CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC 
TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA 
CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA 
GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT 
TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA 
GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT 
AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT 
GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC4 ( XM_005212521.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212578.2 Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220266
Clone ID Related Accession (Same CDS sequence)	XM_005212521.4
Accession Version	XM_005212521.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	NLR family CARD domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212521.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG 
ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA 
ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA 
GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG 
GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT 
CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC 
TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA 
CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA 
GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT 
TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA 
GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT 
AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT 
GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212578.2
CDS	398..3451
Translation	MNFIKENSQVLIQRMGMTVIKQILDELFVWNVMNYEEVNVICGEKFEQDAARGVIHMILKKGSEACNLFLKSLEKWNYPLFQELHGLSLFHQMSEEDLDDLAQELKYFYQSPSFLNFYPLGEDIDIMFNLKSTFTEPVLWKKDQHHHRLEQLTLSGLLDTLQSPCIIEGESGKGKSTLLQRIAMLWASGECQALTKFKLVFFLRLSRAQGGLFETLSDQLLDIPDVISKQTFMARLLKLRQRVLFLLDGYNEFKAQNCPEIEALIKENHRFKNMVIVTTTTESLRHIRQFGALIAEVGDMTESSAQALIQEVLRKEFAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMGKSEFHSHTQTTLFCTFYDLLINKNRHKRKGLAPSEVTQSLDHCGDLALEGVFSRRFDFEPDDLSNVNEDVLLTTGLLCKYTAQRFKPKYKFFHQSFQEYTAGRRLSSLLTSGEPAEVTKGNGHLQKMVSISDITSKYSNLLLYTCGSSAEATRTVLKHLSSVYQHGSLLGLSVTKRPLWRQESMQNMKSTTVQEILKAININSFTECGINLFHESISTSSLSKEFEDFFRGKSLYINSENIPDYLFDFFEDLPNCASALDFVKLDFYGGAVRDISGNQDQEFSGTYIPSRAVSLFFNWKQEFKTLDVTLRDFCKLSKKDIKYLEKIFSSATSLRLHIKRCVGMAGSLSSVLSTCKNIHSLIVEASPLTLEDEQHITSVTNLQTLGVHDLQIQRLPGGLTDNLGNLKNLMKLILDNIQMNEEDALKLAEGLTNLKKMCLLRLTHLSDIGEGMDYIVKSLSAEPCDLKEIQLVSCCLSGNAVKTLAQNLHNLARLSILDLSENHLEKDGKEALQQLIDRLHILEQLTVLMLPWCGDVRVSLARLLEQLERVPQLVKLGLKNWRLTDAEIRILGVFFEKNPLENFQQLDLAGNCVSSDGWLAFMSGFENLKELVFFDFSTKGLLPDASLVRKLSHVLSKLTFLQEVQLVGWQLDDDDVSVLKGAFKLVIA

Target ORF information:

RefSeq Version	XM_005212521.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212521.4

ORF Insert Sequence:

ATGAATTTCA TAAAGGAGAA CAGTCAAGTT CTCATTCAAA GGATGGGAAT GACTGTGATA 
AAGCAAATTT TGGATGAGCT ATTTGTATGG AATGTTATGA ATTACGAAGA AGTAAATGTC 
ATTTGCGGCG AGAAGTTTGA GCAGGACGCT GCGAGAGGGG TCATTCACAT GATTTTGAAG 
AAGGGTTCAG AAGCCTGTAA CCTCTTTCTT AAATCCCTTG AAAAGTGGAA CTATCCTCTG 
TTTCAGGAAT TGCATGGACT AAGTCTTTTT CATCAGATGT CAGAAGAAGA CTTAGATGAT 
TTGGCTCAGG AATTAAAGTA CTTCTATCAG AGCCCATCTT TTCTCAACTT CTATCCCCTG 
GGTGAAGATA TTGACATCAT GTTTAATTTG AAAAGCACCT TCACAGAACC GGTGCTATGG 
AAGAAGGATC AACACCATCA CCGCCTGGAG CAGCTGACCC TGAGTGGCCT GCTGGACACT 
CTTCAGAGCC CCTGCATCAT CGAAGGGGAA TCGGGCAAAG GGAAGTCCAC TCTGCTGCAG 
CGAATCGCCA TGCTCTGGGC CTCTGGAGAG TGCCAGGCTC TCACCAAGTT CAAATTAGTC 
TTCTTTCTTC GTCTGAGCAG GGCCCAGGGT GGACTCTTCG AAACCCTGTC TGACCAACTC 
CTGGATATAC CTGATGTGAT CAGTAAGCAG ACTTTCATGG CCAGGCTGCT GAAGTTACGG 
CAAAGAGTTC TTTTTCTGCT TGATGGCTAC AATGAATTCA AGGCCCAGAA CTGCCCAGAA 
ATTGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAATA TGGTTATCGT CACCACCACC 
ACAGAGAGCC TGAGGCACAT CCGGCAGTTT GGGGCACTGA TTGCTGAGGT GGGGGATATG 
ACTGAGAGCA GTGCCCAGGC TCTCATCCAG GAAGTGCTGA GAAAGGAGTT CGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC CTGAGGAATC TGATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT TCAGATGGGG AAAAGTGAGT TCCACTCTCA CACACAAACC 
ACACTGTTCT GCACCTTCTA CGATCTGCTA ATAAATAAAA ACAGGCACAA ACGTAAAGGG 
CTGGCTCCGA GTGAAGTCAC TCAGAGCTTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC GCAGGTTTGA TTTCGAACCA GACGACTTGT CCAATGTGAA CGAGGATGTC 
CTGCTGACAA CAGGACTCCT CTGTAAATAC ACAGCGCAAC GGTTCAAGCC AAAGTATAAA 
TTCTTTCATC AGTCATTCCA GGAGTACACA GCAGGACGCA GACTCAGCAG TTTACTGACG 
TCTGGAGAAC CAGCGGAGGT AACCAAGGGG AATGGTCACC TGCAGAAAAT GGTTTCCATT 
TCAGACATTA CATCCAAGTA CAGCAACTTG CTCCTGTACA CGTGCGGCTC ATCTGCTGAA 
GCCACCCGGA CTGTTCTGAA ACACTTGTCA TCAGTGTATC AACATGGCAG TCTCCTTGGA 
CTTTCCGTCA CCAAGAGGCC TCTCTGGAGG CAGGAATCTA TGCAAAATAT GAAAAGCACC 
ACTGTGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTACAGAGTG TGGAATCAAC 
TTATTCCATG AGAGTATATC CACATCTAGC CTGAGCAAAG AATTTGAAGA TTTCTTTCGC 
GGTAAAAGCT TATATATCAA CTCAGAGAAT ATCCCTGACT ACTTATTTGA CTTCTTTGAA 
GACTTGCCTA ACTGTGCAAG TGCTCTTGAC TTCGTTAAAC TGGACTTCTA TGGGGGGGCT 
GTGAGAGACA TAAGCGGGAA CCAGGATCAA GAGTTCTCAG GAACCTACAT TCCCAGCAGA 
GCTGTGTCTT TGTTCTTCAA CTGGAAGCAG GAGTTTAAGA CCTTGGATGT CACACTCCGG 
GATTTCTGCA AGTTGAGTAA GAAAGATATC AAATATCTGG AGAAAATATT CAGCTCAGCT 
ACAAGCCTCA GGTTGCACAT TAAGAGATGT GTCGGAATGG CTGGAAGCCT CAGTTCAGTC 
CTCAGCACCT GTAAGAACAT TCATTCCCTC ATAGTGGAAG CCAGTCCCCT TACCCTAGAA 
GACGAGCAGC ATATCACATC TGTGACAAAC CTGCAGACCT TGGGTGTTCA TGACCTACAG 
ATTCAACGGC TGCCAGGTGG TCTGACTGAC AATTTAGGTA ATTTGAAGAA TCTTATGAAG 
CTCATACTGG ATAATATTCA GATGAATGAA GAAGATGCTT TAAAACTAGC TGAAGGTCTG 
ACAAATCTGA AAAAGATGTG CTTACTTCGT TTGACCCACT TGTCTGATAT AGGGGAAGGA 
ATGGATTACA TAGTCAAGTC TCTATCAGCT GAACCCTGTG ACCTTAAAGA AATTCAATTA 
GTCTCCTGCT GCCTGTCAGG AAATGCTGTG AAAACCCTAG CTCAGAATCT TCATAACCTG 
GCCAGACTGA GCATTCTTGA TTTATCTGAA AATCATCTGG AAAAGGATGG AAAAGAAGCT 
CTTCAGCAAC TAATTGACAG GCTGCATATC TTAGAACAGC TAACCGTTCT GATGCTGCCC 
TGGTGTGGTG ATGTACGAGT CAGTCTGGCC AGACTGTTGG AGCAACTGGA GAGGGTCCCA 
CAGCTCGTCA AGCTTGGGCT GAAAAACTGG AGACTCACAG ATGCAGAGAT AAGAATTCTA 
GGTGTATTTT TTGAAAAGAA TCCTCTGGAA AACTTCCAGC AGTTGGATTT GGCAGGAAAT 
TGTGTAAGCA GCGATGGATG GCTTGCCTTC ATGAGTGGAT TTGAGAATCT TAAGGAACTA 
GTGTTTTTTG ACTTCAGTAC GAAAGGACTC TTACCTGATG CATCATTAGT CAGGAAACTT 
AGCCACGTGT TATCCAAACT AACTTTTCTG CAAGAGGTTC AGCTTGTTGG GTGGCAACTT 
GATGACGATG ATGTCAGTGT TCTTAAAGGT GCTTTTAAAC TAGTAATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NLRC4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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