nlrc4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nlrc4
Entrez Gene ID	733844
Full Name	NLR family, CARD domain containing 4
Synonyms	Ipaf
General protein information	Preferred Names NLR family, CARD domain containing 4 Names NLR family CARD domain-containing protein 4 Ice protease-activating factor
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933867.5	XP_002933913.1	NLR family CARD domain-containing protein 4
XM_002933867.4	XP_002933913.1	NLR family CARD domain-containing protein 4

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NLRC4	XP_001105818.2
Bos taurus (cattle)	NLRC4	NP_001179252.2
Homo sapiens (human)	NLRC4	NP_001186067.1
Pan troglodytes (chimpanzee)	NLRC4	XP_515390.2
Xenopus tropicalis (tropical clawed frog)	nlrc4	XP_004914732.1
Mus musculus (house mouse)	Nlrc4	NP_001028539.1
Rattus norvegicus (Norway rat)	Nlrc4	XP_003750157.2

Gene Ontology

The following nlrc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nlrc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10723	XM_002933867.5 Latest version!	Xenopus tropicalis NLR family, CARD domain containing 4 (nlrc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa10723	XM_002933867.4	Xenopus tropicalis NLR family, CARD domain containing 4 (nlrc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nlrc4 ( XM_002933867.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933913.1 Xenopus tropicalis NLR family, CARD domain containing 4 (nlrc4), mRNA.

Price & Availability↑

CloneID	OXa10723
Clone ID Related Accession (Same CDS sequence)	XM_002933867.5 , XM_002933867.4
Accession Version	XM_002933867.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NLR family CARD domain-containing protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933867.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAA TTAGGAAAAA CTATGCAGAG CTGGTTCAGA GAATGGGAAG GACGATTGCT 
GTACAGATAA CTAATGACCT GTTCACAAGA AATATTCTTT CTATGGGTGA GATGGAGGAA 
ATTCTGTCCT GTAAGGTGGC ACAAGATCTA ACAAGAGAAC TGCTTAATGT TATTTTAAAA 
AAAGGCACAG AGTCCTGCAC TCGGTTACTT CAGTCCCTCG AAAATCAAGA TCCTTTTTTC 
TATGAAGACT TGATTGGACA GCGAGTTCAG AGTGGAGTCA CAGAAGAAGA TTTAGAAAAC 
CTAGCTGACC ACTTAAAGCG TCTGTATCAA TACCCCTTTT TCAAGAAATT TAATCCGCTG 
GGTGAGGACA CTGACATCGA TATCATTTTT GATTTAGACC GAACATTTAC AGATCCATTG 
CTCTGGAGGA AAGGAACCCT TAACCGAAGG GAAAAACAGT TGACGTTAAG TGAAATGTTG 
GAACAACTGG AAAGCCCTTG TGTTATTGAA GGAGAAGCAG GGAAGGGGAA AACAACTATA 
CTCAAGAGAA TTGCTATGCT TTGGGCATCT GAAAAATGTA GAGCATTAGC AGATTTTAAA 
CTTGTCTTCT TTGTCACCCT GCGTGGTGCT AGCGAAGGGC TCTATGAAAC ACTCTCTGAC 
CAGCTTTTTC CTATAACTTA CTCATGGAAT AAAAAAGAAT TTCTGAATAA AATATGGCAT 
TTAGGAAGAA AAGTATTGTT CTTGCTTGAT GGGTATGATG AATTTCAGTC GGAGAGCTGC 
ACAGAAATTG AAGAACTTAT TAAAAACAAT CCTAAATTTA ACAGCACAGT GATTGTGAGT 
ACAAGAACAG AGACAATTGG AAAACTGAGG AGGTGTGGTG CGCTTATTGC TGAAACAAGT 
GATTTTTCTT TAGATAATGC TAAACAGTTA ATTGCAAATG TTTTAGAAGA GGATGAAGCA 
AATGGCTTGT TGTTCCAGTT AGAAGAATCA AGTTTTATGC AGAACCTTAT GAAGACCCCC 
TTATTTGTCG TAATTGCTTG CGCTCTTCGG ATGGGGGAAA GCGATTTTCA AATGAACACC 
CAGACAACAC TGTTCAGTAC ATTATATGAC CTAATGGTTG AAAAAAAAAA GTACAAAATT 
AGACACCTTA GTGCAAACAT CCTAGCTGCA AACATCAGGA AATTTGGAGA TCTGGCCCTG 
GATGGGTTGT TTGAACAAAG ATTTGACTTT AATGAGAAGC ATCTGTCTAA AATTAAGGAA 
GAGGTTCTCT TAAATATAGG CCTGCTAAAT AAATACACTG CTCAGAGGCG CAAGGCCGTT 
TACAGATATT TTCATACAAC ATTTCAGGAA TACATTGCCG GGAGGAGACT CTCTCAACTT 
CTATCTTCTG AAGACAACTC AGATGTTACT AAAGGAGAAG ATTTTTTAAA TAAGATTGTT 
TCTGTTTTTG ATATCACCAC TAAATATAAA AATCTCCTCT TATATACTTG TGGATCATCC 
AAAGTAGCAA CACAGAAAGT TGTAAAACAT GTTGCCGAAG TACACAAACA TGACAAAAAT 
AATTACAGTA CAGAGTTGGT AGAGTTTGGG CTTAACCTTT TCTTTGAAAG CTCCACCAAA 
AAAGAATTAA GTCAAGATTT TGAAACTCTC TTTTCTGAGA AATGCTTGTA TATTAATTCC 
CATAATATCT CTTCTCACCA TATTGAATTT TTCCTATACT TACCAAACTG CCTTAGTGCA 
TTACAACTGA TAAAGTTGGA TCTATCTGGT ACTTTTGCCA CTGTACCAAG TGAAAGCACT 
ATGGACAGAG ATTCCAAAAG TGCACAGTCC AGTGTTTGTG ACAATTATAT TCCAGAGAAA 
GCAGTCAAAT TATTTTTTGA TTGGAATCAG TCAATTCAGA CTTTGGAGGT TACACTCAGA 
GATTTTCACC AATTAAATAA AAAAGATATA AAATACCTGG GCAAAATATG TTGCTCCGCT 
GACAGTCTTC GACTGAACAT TAAAAGAAGT TCAGGAATAA CTGGATCCTT GGTGGGTGTT 
TTGGAATCCT GTAAGAATAT ACAAGATTTA AATGTTGATA GCACCAAACT AAGCATTGAA 
GATGAGAGAA GAATTGTACA AATGACGGAG ATGAAAACAC TGAGTATATT AAACCTACAC 
AGTGAACATC TACAAGGTGG ACTATTAGAG GGTTTGTGTA ATCTAGTAGG GCTTGAAAAA 
TTGGTTTTCC ATAACATTAA AATAGACAAG AATGATGCAA AAACTCTGGC TGAAGGCATT 
TTAAGTCTGA AGAAGTTAAA GAGGTTATCC ATATCTCATA TCTCCAACAT TGGAGATGGC 
ATGGAGAGTA TTGCAGAATC AATTTCCTTA TGTTGCCATG AGCTTAAAGA ACTGAAATTA 
ATTGACTGTT GTTTGAGTGC GAAAGCTCTC AGGAGTCTTG CATTCCGCTT AAAAATGTTC 
TCTAGCATTG AAATATTGGA TTTGTCTGGA AATTATTTAC TAGAAGAAGG GAAGGAATCA 
GTAGAAGAAC TGGCTGCCAA CTTGACTCAT CTTGATGCAA TAAGGACACT TATGCTGCCT 
GGAGGAACAG ATGTAAAGTT TTGCCTGGAG GCAGTATTAC CTACATTGAG GCGCATTCCT 
ACCTTGTCAG AACTTGCTTT TAAACGATGG AACTTGACCA ATGATGACTT GATGACATTA 
GCTTCGTATA TAAATAGCGG CTTTGAAAAC CTTTCATTTT TGGATCTTTC TGATAACTGT 
GCTCAAAGTG CCGGCTGGCT TTCCCTGACT GCCATACTAC AATATCTGCC AAATCTGACC 
TATGTAAACT TTAGCACTGA GGACCTCTTC ACTCCAGATC CAGATTTAGT GCGGAAGCTG 
GTTCGTGCAA TATCAGCGCT GCCATTGTTA CACACAATGG AACTAAACAA TTGGCAACTA 
GACGATTTTG ACCTAGCTCA AATCAAAAAA GCAAAAAATA TGATCCACCG GCCTGGGAAT 
ACATGTGGTC ACTTTTTCCA AGTCAGTAAC ATGGAATTTG ATGGAAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933913.1
CDS	944..3994
Translation	MDLIRKNYAELVQRMGRTIAVQITNDLFTRNILSMGEMEEILSCKVAQDLTRELLNVILKKGTESCTRLLQSLENQDPFFYEDLIGQRVQSGVTEEDLENLADHLKRLYQYPFFKKFNPLGEDTDIDIIFDLDRTFTDPLLWRKGTLNRREKQLTLSEMLEQLESPCVIEGEAGKGKTTILKRIAMLWASEKCRALADFKLVFFVTLRGASEGLYETLSDQLFPITYSWNKKEFLNKIWHLGRKVLFLLDGYDEFQSESCTEIEELIKNNPKFNSTVIVSTRTETIGKLRRCGALIAETSDFSLDNAKQLIANVLEEDEANGLLFQLEESSFMQNLMKTPLFVVIACALRMGESDFQMNTQTTLFSTLYDLMVEKKKYKIRHLSANILAANIRKFGDLALDGLFEQRFDFNEKHLSKIKEEVLLNIGLLNKYTAQRRKAVYRYFHTTFQEYIAGRRLSQLLSSEDNSDVTKGEDFLNKIVSVFDITTKYKNLLLYTCGSSKVATQKVVKHVAEVHKHDKNNYSTELVEFGLNLFFESSTKKELSQDFETLFSEKCLYINSHNISSHHIEFFLYLPNCLSALQLIKLDLSGTFATVPSESTMDRDSKSAQSSVCDNYIPEKAVKLFFDWNQSIQTLEVTLRDFHQLNKKDIKYLGKICCSADSLRLNIKRSSGITGSLVGVLESCKNIQDLNVDSTKLSIEDERRIVQMTEMKTLSILNLHSEHLQGGLLEGLCNLVGLEKLVFHNIKIDKNDAKTLAEGILSLKKLKRLSISHISNIGDGMESIAESISLCCHELKELKLIDCCLSAKALRSLAFRLKMFSSIEILDLSGNYLLEEGKESVEELAANLTHLDAIRTLMLPGGTDVKFCLEAVLPTLRRIPTLSELAFKRWNLTNDDLMTLASYINSGFENLSFLDLSDNCAQSAGWLSLTAILQYLPNLTYVNFSTEDLFTPDPDLVRKLVRAISALPLLHTMELNNWQLDDFDLAQIKKAKNMIHRPGNTCGHFFQVSNMEFDGR

Target ORF information:

RefSeq Version	XM_002933867.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NLR family, CARD domain containing 4 (nlrc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933867.5

ORF Insert Sequence:

ATGGATTTAA TTAGGAAAAA CTATGCAGAG CTGGTTCAGA GAATGGGAAG GACGATTGCT 
GTACAGATAA CTAATGACCT GTTCACAAGA AATATTCTTT CTATGGGTGA GATGGAGGAA 
ATTCTGTCCT GTAAGGTGGC ACAAGATCTA ACAAGAGAAC TGCTTAATGT TATTTTAAAA 
AAAGGCACAG AGTCCTGCAC TCGGTTACTT CAGTCCCTCG AAAATCAAGA TCCTTTTTTC 
TATGAAGACT TGATTGGACA GCGAGTTCAG AGTGGAGTCA CAGAAGAAGA TTTAGAAAAC 
CTAGCTGACC ACTTAAAGCG TCTGTATCAA TACCCCTTTT TCAAGAAATT TAATCCGCTG 
GGTGAGGACA CTGACATCGA TATCATTTTT GATTTAGACC GAACATTTAC AGATCCATTG 
CTCTGGAGGA AAGGAACCCT TAACCGAAGG GAAAAACAGT TGACGTTAAG TGAAATGTTG 
GAACAACTGG AAAGCCCTTG TGTTATTGAA GGAGAAGCAG GGAAGGGGAA AACAACTATA 
CTCAAGAGAA TTGCTATGCT TTGGGCATCT GAAAAATGTA GAGCATTAGC AGATTTTAAA 
CTTGTCTTCT TTGTCACCCT GCGTGGTGCT AGCGAAGGGC TCTATGAAAC ACTCTCTGAC 
CAGCTTTTTC CTATAACTTA CTCATGGAAT AAAAAAGAAT TTCTGAATAA AATATGGCAT 
TTAGGAAGAA AAGTATTGTT CTTGCTTGAT GGGTATGATG AATTTCAGTC GGAGAGCTGC 
ACAGAAATTG AAGAACTTAT TAAAAACAAT CCTAAATTTA ACAGCACAGT GATTGTGAGT 
ACAAGAACAG AGACAATTGG AAAACTGAGG AGGTGTGGTG CGCTTATTGC TGAAACAAGT 
GATTTTTCTT TAGATAATGC TAAACAGTTA ATTGCAAATG TTTTAGAAGA GGATGAAGCA 
AATGGCTTGT TGTTCCAGTT AGAAGAATCA AGTTTTATGC AGAACCTTAT GAAGACCCCC 
TTATTTGTCG TAATTGCTTG CGCTCTTCGG ATGGGGGAAA GCGATTTTCA AATGAACACC 
CAGACAACAC TGTTCAGTAC ATTATATGAC CTAATGGTTG AAAAAAAAAA GTACAAAATT 
AGACACCTTA GTGCAAACAT CCTAGCTGCA AACATCAGGA AATTTGGAGA TCTGGCCCTG 
GATGGGTTGT TTGAACAAAG ATTTGACTTT AATGAGAAGC ATCTGTCTAA AATTAAGGAA 
GAGGTTCTCT TAAATATAGG CCTGCTAAAT AAATACACTG CTCAGAGGCG CAAGGCCGTT 
TACAGATATT TTCATACAAC ATTTCAGGAA TACATTGCCG GGAGGAGACT CTCTCAACTT 
CTATCTTCTG AAGACAACTC AGATGTTACT AAAGGAGAAG ATTTTTTAAA TAAGATTGTT 
TCTGTTTTTG ATATCACCAC TAAATATAAA AATCTCCTCT TATATACTTG TGGATCATCC 
AAAGTAGCAA CACAGAAAGT TGTAAAACAT GTTGCCGAAG TACACAAACA TGACAAAAAT 
AATTACAGTA CAGAGTTGGT AGAGTTTGGG CTTAACCTTT TCTTTGAAAG CTCCACCAAA 
AAAGAATTAA GTCAAGATTT TGAAACTCTC TTTTCTGAGA AATGCTTGTA TATTAATTCC 
CATAATATCT CTTCTCACCA TATTGAATTT TTCCTATACT TACCAAACTG CCTTAGTGCA 
TTACAACTGA TAAAGTTGGA TCTATCTGGT ACTTTTGCCA CTGTACCAAG TGAAAGCACT 
ATGGACAGAG ATTCCAAAAG TGCACAGTCC AGTGTTTGTG ACAATTATAT TCCAGAGAAA 
GCAGTCAAAT TATTTTTTGA TTGGAATCAG TCAATTCAGA CTTTGGAGGT TACACTCAGA 
GATTTTCACC AATTAAATAA AAAAGATATA AAATACCTGG GCAAAATATG TTGCTCCGCT 
GACAGTCTTC GACTGAACAT TAAAAGAAGT TCAGGAATAA CTGGATCCTT GGTGGGTGTT 
TTGGAATCCT GTAAGAATAT ACAAGATTTA AATGTTGATA GCACCAAACT AAGCATTGAA 
GATGAGAGAA GAATTGTACA AATGACGGAG ATGAAAACAC TGAGTATATT AAACCTACAC 
AGTGAACATC TACAAGGTGG ACTATTAGAG GGTTTGTGTA ATCTAGTAGG GCTTGAAAAA 
TTGGTTTTCC ATAACATTAA AATAGACAAG AATGATGCAA AAACTCTGGC TGAAGGCATT 
TTAAGTCTGA AGAAGTTAAA GAGGTTATCC ATATCTCATA TCTCCAACAT TGGAGATGGC 
ATGGAGAGTA TTGCAGAATC AATTTCCTTA TGTTGCCATG AGCTTAAAGA ACTGAAATTA 
ATTGACTGTT GTTTGAGTGC GAAAGCTCTC AGGAGTCTTG CATTCCGCTT AAAAATGTTC 
TCTAGCATTG AAATATTGGA TTTGTCTGGA AATTATTTAC TAGAAGAAGG GAAGGAATCA 
GTAGAAGAAC TGGCTGCCAA CTTGACTCAT CTTGATGCAA TAAGGACACT TATGCTGCCT 
GGAGGAACAG ATGTAAAGTT TTGCCTGGAG GCAGTATTAC CTACATTGAG GCGCATTCCT 
ACCTTGTCAG AACTTGCTTT TAAACGATGG AACTTGACCA ATGATGACTT GATGACATTA 
GCTTCGTATA TAAATAGCGG CTTTGAAAAC CTTTCATTTT TGGATCTTTC TGATAACTGT 
GCTCAAAGTG CCGGCTGGCT TTCCCTGACT GCCATACTAC AATATCTGCC AAATCTGACC 
TATGTAAACT TTAGCACTGA GGACCTCTTC ACTCCAGATC CAGATTTAGT GCGGAAGCTG 
GTTCGTGCAA TATCAGCGCT GCCATTGTTA CACACAATGG AACTAAACAA TTGGCAACTA 
GACGATTTTG ACCTAGCTCA AATCAAAAAA GCAAAAAATA TGATCCACCG GCCTGGGAAT 
ACATGTGGTC ACTTTTTCCA AGTCAGTAAC ATGGAATTTG ATGGAAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlrc4 ( XM_002933867.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933913.1 Xenopus tropicalis NLR family, CARD domain containing 4 (nlrc4), mRNA.

Price & Availability↑

CloneID	OXa10723
Clone ID Related Accession (Same CDS sequence)	XM_002933867.5 , XM_002933867.4
Accession Version	XM_002933867.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NLR family CARD domain-containing protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAA TTAGGAAAAA CTATGCAGAG CTGGTTCAGA GAATGGGAAG GACGATTGCT 
GTACAGATAA CTAATGACCT GTTCACAAGA AATATTCTTT CTATGGGTGA GATGGAGGAA 
ATTCTGTCCT GTAAGGTGGC ACAAGATCTA ACAAGAGAAC TGCTTAATGT TATTTTAAAA 
AAAGGCACAG AGTCCTGCAC TCGGTTACTT CAGTCCCTCG AAAATCAAGA TCCTTTTTTC 
TATGAAGACT TGATTGGACA GCGAGTTCAG AGTGGAGTCA CAGAAGAAGA TTTAGAAAAC 
CTAGCTGACC ACTTAAAGCG TCTGTATCAA TACCCCTTTT TCAAGAAATT TAATCCGCTG 
GGTGAGGACA CTGACATCGA TATCATTTTT GATTTAGACC GAACATTTAC AGATCCATTG 
CTCTGGAGGA AAGGAACCCT TAACCGAAGG GAAAAACAGT TGACGTTAAG TGAAATGTTG 
GAACAACTGG AAAGCCCTTG TGTTATTGAA GGAGAAGCAG GGAAGGGGAA AACAACTATA 
CTCAAGAGAA TTGCTATGCT TTGGGCATCT GAAAAATGTA GAGCATTAGC AGATTTTAAA 
CTTGTCTTCT TTGTCACCCT GCGTGGTGCT AGCGAAGGGC TCTATGAAAC ACTCTCTGAC 
CAGCTTTTTC CTATAACTTA CTCATGGAAT AAAAAAGAAT TTCTGAATAA AATATGGCAT 
TTAGGAAGAA AAGTATTGTT CTTGCTTGAT GGGTATGATG AATTTCAGTC GGAGAGCTGC 
ACAGAAATTG AAGAACTTAT TAAAAACAAT CCTAAATTTA ACAGCACAGT GATTGTGAGT 
ACAAGAACAG AGACAATTGG AAAACTGAGG AGGTGTGGTG CGCTTATTGC TGAAACAAGT 
GATTTTTCTT TAGATAATGC TAAACAGTTA ATTGCAAATG TTTTAGAAGA GGATGAAGCA 
AATGGCTTGT TGTTCCAGTT AGAAGAATCA AGTTTTATGC AGAACCTTAT GAAGACCCCC 
TTATTTGTCG TAATTGCTTG CGCTCTTCGG ATGGGGGAAA GCGATTTTCA AATGAACACC 
CAGACAACAC TGTTCAGTAC ATTATATGAC CTAATGGTTG AAAAAAAAAA GTACAAAATT 
AGACACCTTA GTGCAAACAT CCTAGCTGCA AACATCAGGA AATTTGGAGA TCTGGCCCTG 
GATGGGTTGT TTGAACAAAG ATTTGACTTT AATGAGAAGC ATCTGTCTAA AATTAAGGAA 
GAGGTTCTCT TAAATATAGG CCTGCTAAAT AAATACACTG CTCAGAGGCG CAAGGCCGTT 
TACAGATATT TTCATACAAC ATTTCAGGAA TACATTGCCG GGAGGAGACT CTCTCAACTT 
CTATCTTCTG AAGACAACTC AGATGTTACT AAAGGAGAAG ATTTTTTAAA TAAGATTGTT 
TCTGTTTTTG ATATCACCAC TAAATATAAA AATCTCCTCT TATATACTTG TGGATCATCC 
AAAGTAGCAA CACAGAAAGT TGTAAAACAT GTTGCCGAAG TACACAAACA TGACAAAAAT 
AATTACAGTA CAGAGTTGGT AGAGTTTGGG CTTAACCTTT TCTTTGAAAG CTCCACCAAA 
AAAGAATTAA GTCAAGATTT TGAAACTCTC TTTTCTGAGA AATGCTTGTA TATTAATTCC 
CATAATATCT CTTCTCACCA TATTGAATTT TTCCTATACT TACCAAACTG CCTTAGTGCA 
TTACAACTGA TAAAGTTGGA TCTATCTGGT ACTTTTGCCA CTGTACCAAG TGAAAGCACT 
ATGGACAGAG ATTCCAAAAG TGCACAGTCC AGTGTTTGTG ACAATTATAT TCCAGAGAAA 
GCAGTCAAAT TATTTTTTGA TTGGAATCAG TCAATTCAGA CTTTGGAGGT TACACTCAGA 
GATTTTCACC AATTAAATAA AAAAGATATA AAATACCTGG GCAAAATATG TTGCTCCGCT 
GACAGTCTTC GACTGAACAT TAAAAGAAGT TCAGGAATAA CTGGATCCTT GGTGGGTGTT 
TTGGAATCCT GTAAGAATAT ACAAGATTTA AATGTTGATA GCACCAAACT AAGCATTGAA 
GATGAGAGAA GAATTGTACA AATGACGGAG ATGAAAACAC TGAGTATATT AAACCTACAC 
AGTGAACATC TACAAGGTGG ACTATTAGAG GGTTTGTGTA ATCTAGTAGG GCTTGAAAAA 
TTGGTTTTCC ATAACATTAA AATAGACAAG AATGATGCAA AAACTCTGGC TGAAGGCATT 
TTAAGTCTGA AGAAGTTAAA GAGGTTATCC ATATCTCATA TCTCCAACAT TGGAGATGGC 
ATGGAGAGTA TTGCAGAATC AATTTCCTTA TGTTGCCATG AGCTTAAAGA ACTGAAATTA 
ATTGACTGTT GTTTGAGTGC GAAAGCTCTC AGGAGTCTTG CATTCCGCTT AAAAATGTTC 
TCTAGCATTG AAATATTGGA TTTGTCTGGA AATTATTTAC TAGAAGAAGG GAAGGAATCA 
GTAGAAGAAC TGGCTGCCAA CTTGACTCAT CTTGATGCAA TAAGGACACT TATGCTGCCT 
GGAGGAACAG ATGTAAAGTT TTGCCTGGAG GCAGTATTAC CTACATTGAG GCGCATTCCT 
ACCTTGTCAG AACTTGCTTT TAAACGATGG AACTTGACCA ATGATGACTT GATGACATTA 
GCTTCGTATA TAAATAGCGG CTTTGAAAAC CTTTCATTTT TGGATCTTTC TGATAACTGT 
GCTCAAAGTG CCGGCTGGCT TTCCCTGACT GCCATACTAC AATATCTGCC AAATCTGACC 
TATGTAAACT TTAGCACTGA GGACCTCTTC ACTCCAGATC CAGATTTAGT GCGGAAGCTG 
GTTCGTGCAA TATCAGCGCT GCCATTGTTA CACACAATGG AACTAAACAA TTGGCAACTA 
GACGATTTTG ACCTAGCTCA AATCAAAAAA GCAAAAAATA TGATCCACCG GCCTGGGAAT 
ACATGTGGTC ACTTTTTCCA AGTCAGTAAC ATGGAATTTG ATGGAAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933913.1
CDS	1381..4431
Translation	MDLIRKNYAELVQRMGRTIAVQITNDLFTRNILSMGEMEEILSCKVAQDLTRELLNVILKKGTESCTRLLQSLENQDPFFYEDLIGQRVQSGVTEEDLENLADHLKRLYQYPFFKKFNPLGEDTDIDIIFDLDRTFTDPLLWRKGTLNRREKQLTLSEMLEQLESPCVIEGEAGKGKTTILKRIAMLWASEKCRALADFKLVFFVTLRGASEGLYETLSDQLFPITYSWNKKEFLNKIWHLGRKVLFLLDGYDEFQSESCTEIEELIKNNPKFNSTVIVSTRTETIGKLRRCGALIAETSDFSLDNAKQLIANVLEEDEANGLLFQLEESSFMQNLMKTPLFVVIACALRMGESDFQMNTQTTLFSTLYDLMVEKKKYKIRHLSANILAANIRKFGDLALDGLFEQRFDFNEKHLSKIKEEVLLNIGLLNKYTAQRRKAVYRYFHTTFQEYIAGRRLSQLLSSEDNSDVTKGEDFLNKIVSVFDITTKYKNLLLYTCGSSKVATQKVVKHVAEVHKHDKNNYSTELVEFGLNLFFESSTKKELSQDFETLFSEKCLYINSHNISSHHIEFFLYLPNCLSALQLIKLDLSGTFATVPSESTMDRDSKSAQSSVCDNYIPEKAVKLFFDWNQSIQTLEVTLRDFHQLNKKDIKYLGKICCSADSLRLNIKRSSGITGSLVGVLESCKNIQDLNVDSTKLSIEDERRIVQMTEMKTLSILNLHSEHLQGGLLEGLCNLVGLEKLVFHNIKIDKNDAKTLAEGILSLKKLKRLSISHISNIGDGMESIAESISLCCHELKELKLIDCCLSAKALRSLAFRLKMFSSIEILDLSGNYLLEEGKESVEELAANLTHLDAIRTLMLPGGTDVKFCLEAVLPTLRRIPTLSELAFKRWNLTNDDLMTLASYINSGFENLSFLDLSDNCAQSAGWLSLTAILQYLPNLTYVNFSTEDLFTPDPDLVRKLVRAISALPLLHTMELNNWQLDDFDLAQIKKAKNMIHRPGNTCGHFFQVSNMEFDGR

Target ORF information:

RefSeq Version	XM_002933867.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NLR family, CARD domain containing 4 (nlrc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933867.4

ORF Insert Sequence:

ATGGATTTAA TTAGGAAAAA CTATGCAGAG CTGGTTCAGA GAATGGGAAG GACGATTGCT 
GTACAGATAA CTAATGACCT GTTCACAAGA AATATTCTTT CTATGGGTGA GATGGAGGAA 
ATTCTGTCCT GTAAGGTGGC ACAAGATCTA ACAAGAGAAC TGCTTAATGT TATTTTAAAA 
AAAGGCACAG AGTCCTGCAC TCGGTTACTT CAGTCCCTCG AAAATCAAGA TCCTTTTTTC 
TATGAAGACT TGATTGGACA GCGAGTTCAG AGTGGAGTCA CAGAAGAAGA TTTAGAAAAC 
CTAGCTGACC ACTTAAAGCG TCTGTATCAA TACCCCTTTT TCAAGAAATT TAATCCGCTG 
GGTGAGGACA CTGACATCGA TATCATTTTT GATTTAGACC GAACATTTAC AGATCCATTG 
CTCTGGAGGA AAGGAACCCT TAACCGAAGG GAAAAACAGT TGACGTTAAG TGAAATGTTG 
GAACAACTGG AAAGCCCTTG TGTTATTGAA GGAGAAGCAG GGAAGGGGAA AACAACTATA 
CTCAAGAGAA TTGCTATGCT TTGGGCATCT GAAAAATGTA GAGCATTAGC AGATTTTAAA 
CTTGTCTTCT TTGTCACCCT GCGTGGTGCT AGCGAAGGGC TCTATGAAAC ACTCTCTGAC 
CAGCTTTTTC CTATAACTTA CTCATGGAAT AAAAAAGAAT TTCTGAATAA AATATGGCAT 
TTAGGAAGAA AAGTATTGTT CTTGCTTGAT GGGTATGATG AATTTCAGTC GGAGAGCTGC 
ACAGAAATTG AAGAACTTAT TAAAAACAAT CCTAAATTTA ACAGCACAGT GATTGTGAGT 
ACAAGAACAG AGACAATTGG AAAACTGAGG AGGTGTGGTG CGCTTATTGC TGAAACAAGT 
GATTTTTCTT TAGATAATGC TAAACAGTTA ATTGCAAATG TTTTAGAAGA GGATGAAGCA 
AATGGCTTGT TGTTCCAGTT AGAAGAATCA AGTTTTATGC AGAACCTTAT GAAGACCCCC 
TTATTTGTCG TAATTGCTTG CGCTCTTCGG ATGGGGGAAA GCGATTTTCA AATGAACACC 
CAGACAACAC TGTTCAGTAC ATTATATGAC CTAATGGTTG AAAAAAAAAA GTACAAAATT 
AGACACCTTA GTGCAAACAT CCTAGCTGCA AACATCAGGA AATTTGGAGA TCTGGCCCTG 
GATGGGTTGT TTGAACAAAG ATTTGACTTT AATGAGAAGC ATCTGTCTAA AATTAAGGAA 
GAGGTTCTCT TAAATATAGG CCTGCTAAAT AAATACACTG CTCAGAGGCG CAAGGCCGTT 
TACAGATATT TTCATACAAC ATTTCAGGAA TACATTGCCG GGAGGAGACT CTCTCAACTT 
CTATCTTCTG AAGACAACTC AGATGTTACT AAAGGAGAAG ATTTTTTAAA TAAGATTGTT 
TCTGTTTTTG ATATCACCAC TAAATATAAA AATCTCCTCT TATATACTTG TGGATCATCC 
AAAGTAGCAA CACAGAAAGT TGTAAAACAT GTTGCCGAAG TACACAAACA TGACAAAAAT 
AATTACAGTA CAGAGTTGGT AGAGTTTGGG CTTAACCTTT TCTTTGAAAG CTCCACCAAA 
AAAGAATTAA GTCAAGATTT TGAAACTCTC TTTTCTGAGA AATGCTTGTA TATTAATTCC 
CATAATATCT CTTCTCACCA TATTGAATTT TTCCTATACT TACCAAACTG CCTTAGTGCA 
TTACAACTGA TAAAGTTGGA TCTATCTGGT ACTTTTGCCA CTGTACCAAG TGAAAGCACT 
ATGGACAGAG ATTCCAAAAG TGCACAGTCC AGTGTTTGTG ACAATTATAT TCCAGAGAAA 
GCAGTCAAAT TATTTTTTGA TTGGAATCAG TCAATTCAGA CTTTGGAGGT TACACTCAGA 
GATTTTCACC AATTAAATAA AAAAGATATA AAATACCTGG GCAAAATATG TTGCTCCGCT 
GACAGTCTTC GACTGAACAT TAAAAGAAGT TCAGGAATAA CTGGATCCTT GGTGGGTGTT 
TTGGAATCCT GTAAGAATAT ACAAGATTTA AATGTTGATA GCACCAAACT AAGCATTGAA 
GATGAGAGAA GAATTGTACA AATGACGGAG ATGAAAACAC TGAGTATATT AAACCTACAC 
AGTGAACATC TACAAGGTGG ACTATTAGAG GGTTTGTGTA ATCTAGTAGG GCTTGAAAAA 
TTGGTTTTCC ATAACATTAA AATAGACAAG AATGATGCAA AAACTCTGGC TGAAGGCATT 
TTAAGTCTGA AGAAGTTAAA GAGGTTATCC ATATCTCATA TCTCCAACAT TGGAGATGGC 
ATGGAGAGTA TTGCAGAATC AATTTCCTTA TGTTGCCATG AGCTTAAAGA ACTGAAATTA 
ATTGACTGTT GTTTGAGTGC GAAAGCTCTC AGGAGTCTTG CATTCCGCTT AAAAATGTTC 
TCTAGCATTG AAATATTGGA TTTGTCTGGA AATTATTTAC TAGAAGAAGG GAAGGAATCA 
GTAGAAGAAC TGGCTGCCAA CTTGACTCAT CTTGATGCAA TAAGGACACT TATGCTGCCT 
GGAGGAACAG ATGTAAAGTT TTGCCTGGAG GCAGTATTAC CTACATTGAG GCGCATTCCT 
ACCTTGTCAG AACTTGCTTT TAAACGATGG AACTTGACCA ATGATGACTT GATGACATTA 
GCTTCGTATA TAAATAGCGG CTTTGAAAAC CTTTCATTTT TGGATCTTTC TGATAACTGT 
GCTCAAAGTG CCGGCTGGCT TTCCCTGACT GCCATACTAC AATATCTGCC AAATCTGACC 
TATGTAAACT TTAGCACTGA GGACCTCTTC ACTCCAGATC CAGATTTAGT GCGGAAGCTG 
GTTCGTGCAA TATCAGCGCT GCCATTGTTA CACACAATGG AACTAAACAA TTGGCAACTA 
GACGATTTTG ACCTAGCTCA AATCAAAAAA GCAAAAAATA TGATCCACCG GCCTGGGAAT 
ACATGTGGTC ACTTTTTCCA AGTCAGTAAC ATGGAATTTG ATGGAAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nlrc4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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