Prag1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prag1
Entrez Gene ID	244418
Full Name	PEAK1 related kinase activating pseudokinase 1
Synonyms	9830148H23,D8Ertd82e,NACK,Sgk223,mFLJ00269
General protein information	Preferred Names PEAK1 related kinase activating pseudokinase 1 Names tyrosine-protein kinase PRAG1 tyrosine-protein kinase SgK223 pragmin sugen kinase 223
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A4\|8 22.78 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242137.1	XP_011240439.1	tyrosine-protein kinase SgK223 isoform X1
NM_172911.3	NP_766499.2	inactive tyrosine-protein kinase PRAG1
XM_011242137.2	XP_011240439.1	inactive tyrosine-protein kinase PRAG1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC533821	XP_005226093.1
Canis lupus familiaris (dog)	LOC482885	XP_540000.3
Xenopus tropicalis (tropical clawed frog)	LOC100486686	XP_002936500.1
Mus musculus (house mouse)	D8Ertd82e	NP_766499.2
Rattus norvegicus (Norway rat)	Pragmin	NP_001100785.1
Homo sapiens (human)	SGK223	NP_001074295.1
Macaca mulatta (Rhesus monkey)	LOC706349	XP_001094718.1
Pan troglodytes (chimpanzee)	LOC472692	XP_001136236.1
Gallus gallus (chicken)	SGK223	XP_420588.4

Gene Ontology
Bibliography

Related articles in PubMed

NACK is an integral component of the Notch transcriptional activation complex and is critical for development and tumorigenesis.
Weaver KL, Alves-Guerra MC, Jin K, Wang Z, Han X, Ranganathan P, Zhu X, DaSilva T, Liu W, Ratti F, Demarest RM, Tzimas C, Rice M, Vasquez-Del Carpio R, Dahmane N, Robbins DJ, Capobianco AJ
Cancer research74(17)4741-51(2014 Sep)

Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity.
Tanaka H, Katoh H, Negishi M
The Journal of biological chemistry281(15)10355-64(2006 Apr)

The mouse kinome: discovery and comparative genomics of all mouse protein kinases.
Caenepeel S, Charydczak G, Sudarsanam S, Hunter T, Manning G
Proceedings of the National Academy of Sciences of the United States of America101(32)11707-12(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21183	NM_172911.3 Latest version!	Mus musculus PEAK1 related kinase activating pseudokinase 1 (Prag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu21183	XM_011242137.2 Latest version!	Mus musculus PEAK1 related kinase activating pseudokinase 1 (Prag1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMu21183	XM_011242137.1	Mus musculus DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prag1 ( NM_172911.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766499.2 Mus musculus PEAK1 related kinase activating pseudokinase 1 (Prag1), mRNA.

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CloneID	OMu21183
Clone ID Related Accession (Same CDS sequence)	XM_011242137.1 , NM_172911.3 , XM_011242137.2
Accession Version	NM_172911.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	inactive tyrosine-protein kinase PRAG1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121858.3, AK052494.1, BC089024.1, AK036672.1 and CO042744.1. On Nov 29, 2007 this sequence version replaced NM_172911.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK036672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCGT GCAGCGACTT TGTGGAGCAC ATCTGGAAAC CCGGGTCTTG CAAGAACTGC 
TTCTGCTTGC GAAGTGATCA TCAGCCAACA GCTGGCCATC CCAAGGCTAG AGCCAACAGC 
CTACCCGCTG GAACTCGCCT GCCTGCCAGG CCTGAGAATT GCCGCCTAGA CGACGAAGGC 
GTGAATGGCT TGGCCTACTC AAAGCCCACC ATTGCTGTAA AGCCCACCAT GATGACTTCT 
GAGACCTCCG ACTTGTGGAC TGAAGCCAGC CTGAGCGCTG AAGTCCCAAA GGTCAATTGG 
AGACGAACCC CAGGCAAGCT CCTGCTCCCG AAACAGGAAG ATGAGCCAAT AGTTTACCTA 
GGCAGCTTCC GAGGACTGCA GAAGCCTGCA AGCCCTCTTG CCTGCACAGA TGGCAACTCT 
CGTTGTCCCC CAGCCTATAC CATGGTCGGA CTGCATAACC TGGAGGCCCG CGTGGATAGG 
AACACTGCCT TTCAACCAGT GAGCTTCCAG GAAGAGAAGG CTGGGAGGGA AGAGCTGCCC 
TCAGCTCACG AGAGCTTTCG CCAGAAGCTG GCTGCCTTTG CAGGGATGAC ATCCAGCTGC 
CCCAAGGGCC CCAGGCCCTG CACTTCTCCA CAGCCCCTGC GGGAATCCCT GCCCTCGGAG 
GATGACAGTG ACCAAAGGTG CTCACCCTCA GGAGACAGCG AAGGTGGGGA GTACTGTTCC 
ATTCTGGACT GTTGTCCAGA ATCCAAAGAT GCTGTGCATA GCACCGAGGG CTCTGGCAGA 
CGTGGAGGGG ACTGCTCACC CACGTGCAGG GAGCAGGGAC CACGTACAAG ACCAACAGAA 
GAGGAAAAAC AGGGTCTGAG CTTCCCCAGA GAGTGCTGTG GTCAGGGATC CACAGCGAAC 
CCGCCCCGCC TGGGCCCCAA GAAGCCATCC CTTAACTCTG AGGCCGCCAG CTCTTCCGAT 
GGTCTGTCCT GTGGCAGCAG CCGCAGCGGG GCCAGCAGCC CCTTCGCTCC CCACCTTGAG 
AACGACTACT GCTCTCTTGT GAAGGAACCG GCCTCAGGGA AGCAGCAAGA CCTCAGCGGC 
CACTTTCTCA CCTCTGGCAA ATGTGTGGGT CAAGCTGCGG AACTCCAGCC CGCCTCCCTT 
CTTAGGGACC CTGTGCAGCC TGAACCCATC TACGCCGAAA GCGCCAAGAG GAAGAAGGCA 
GCTCCGGGGC CTCCGAGGCC CGAGCCCAAG AAAGAGCAGG TCCCAGCTGG CCATAGCCAG 
GGCCAAGTAT GGACAGGTGA TACCTGGATT CAGAAGACAC CCCCTAGCTG GAGCCAGGAC 
AGGGAAGGCG CAAACCCTGC TCCTCAGGTG GCAACCACCA TCACTGTCAT CGCTGCTCAC 
CCGGAAGAGG ACCACAGGAC TATTTATCTG AGCAGCCCTG ACTCTGCCGT GGGGGTGCAG 
TGGCCACGTG GGCCTTCGAA CCAGGACTTA CAAGCCGGTG AAGAGGAGCC TTTGGTTGCG 
CAGGGGCTAA CCTCAAGGGA GAGCCATCCA CATAATGTGA CTGAAAACAC GGCCAAAGAG 
AAGCCTGCCA TTCCCCCCAA ACTGTCCAAA AGCAGCCCCG GAGGGTCCCC TGTGTCTCCC 
GCGCCCCCAC TGACTGACCA CAGCGACGGG AACACCGGTG GCAGCAGCGT CGGACCCCAA 
CTTTTGTCTA GAGTCCCTGC TAACCTAACG TCTTCCTGCC ACACCAATGG CGTTGCCACT 
GCTGGTGACT CTGCCAAGTG TCCCCCACCA GCCACCTCCT CTTCAGTCTT GGACCAGAGG 
CGGCCAAGAT ACCAGACTGG TGCTTGGAGC CGCCAGTGCC GGATAGAAGA GGAGGAGGAG 
GTGGGGCAGG AGTTGAGTCA AAGTTGGGGG AGAGAGTTGG AAAACGGTAC CGCAGACCAT 
TCAAACTCCT CTACCTGGCA CCGTCTCCAT CCCATCGATG GCACCTCTGG GCAGAACAGC 
AAAACTAACT CTGGGATGAG CAAATCGGCT TCCTTTGCGT TTGAGTTCCC AAAGGACAGA 
GGCAGATTGG AGGCATTCTC ACCCCCTCCC CCACCTCCAA AGTCAAGGCA CCTGTTAAAA 
ATGAACAAGA GCAGTTCTGA CTTGGAAAAA GTGAGCCAGA GTTCTGCAGA AAGCCTCAGC 
CCGTCCTTCA GAGGGGCCCA CGTCAGCTTC ACCACGGGCT CCACAGATAG CCTAGCCTCG 
GACTCCAGGC CCTGCAGCGA TGGAGGTCCA TCATATGAAC CCACCCACTC ACCCACCATC 
AGCGGGAAAA AGCTCTTCGC TCCCGTCCCT TTTCCATCAG GCTCCACAGA GGATGTGTCC 
CCTGGTGGTG GTCCTGCGCA GCCTCCTCCT CTGCCCCAGA AAAAGATAGT GAGCCGTGCC 
GCCTCTTCCC CTGATGGCTT CTTCTGGACC CAAGGTTCCC CTAAGCCACG GACGGCAAGT 
CCCAAGCTGA ACCTAAGCCA TTCAGAAACC AATGTTTGTG CCCATGACGA GCCTCCTTTT 
AACTGTTCCT TGAACTCTGG AAACCGTTCC CACCATGTCT TCTCCTCTTC TGAGCCTTTG 
GGGAAAGCTT TCAAAGGCAA TGCTCCCTGG GCCCCAGCTC TGGGGCTGGC GAACAGCAAA 
GGCGGCTGTG GGAGCCCCAG TCTCCAGTGC AGAGCGGCCA CCTCCACGTC ATCATCCCAG 
CTCAGCGTGT CCAGCCAGGC CTCCTCCAGC AGCACCCAGC TTCAGCTGCA CAGCCTGCTG 
AGTAGCATCA GCAGCAAGGA GGGCACCTAT GCCAAGCTTG GGGGCCTCTA CACACAATCC 
CTGGCCCGCC TAGTGACCAA GTGTGAAGAT CTCTTCATGG GTGGCCAGAA AAAGGAACTG 
CGCTTCAATG AGAATTACTG GTCACTCTTC AAGCTGACGT GCAACAAACC TTGCTGTGAC 
TCGGGGGATG CAATTTACTA CTGTGCCACC TGCTCTGAGG ACCCAGGCAG CATCTATGCT 
GTGAAAATCT GCAAAACCCC AGAACCCAAA TCAGCCTCAT ACTGCAGCCC ATCTGTGCCG 
GTGCATTTCA ACATCCAGCA AGACTGCGGC CACTTTGTGG CCTCTGTGCC CTCCAGCATG 
CTTGCCTCCC CTGACACATC CAGCAAAGAC ACCGCGCCTG CCGTATCCCC ACAGCCCCCT 
GCCCAGGAGC AGGACTGTGT GGTGGTCATC ACCCGGGAGG TACCGCACCA GACTGCCTCC 
GACTTTGTGC GGGACTCTAT GGCCAGCCAC CGGGCGGAGC CTGAGGTTTA CGAGCGCCGG 
GTGTGCTTCC TGCTTCTGCA GCTCTGCAAC GGGCTGGAGC ACCTGAAGGA ACACGGGATC 
ATCCACCGGG ACCTGTGTCT GGAGAACCTG CTGCTGGCGC ACTGTAACCC TCAGAGCTCC 
CCCGGGCCCT CTGCCACTCC TACCGTGCCC ACCACCACAT CCAGGTGCCC TTCTGCCGCG 
CCCGCAGCCA CCACTGCCTG CCAGGGAGGG CCTGGTGAGA AGCAGCTGCC CAGGCTCATC 
ATCAGCAACT TCCTGAAGGC CAAGCAAAAG CCAGGAGGCA CCACCAACCT GCAACAGAAG 
AAGAGCCAGG CCCGGCTGGC TCCCGAGATC GTGTCTGCCT CCCAGTACCG CAAGTTTGAT 
GAGTTCCAGA CGGGCATCCT CATCTATGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGAGCCCAGC TCCGGGAGCG CGACTACCGG CGGGAGGACT TACCCCCGCT GCCCACACTG 
TCCCTCTACT CTCCCGGCCT GCAGCAACTC GCCCATCTAC TGCTTGAGGC TGATCCCATC 
AAGCGCATCC GCATAGGTGA AGCTAAGCGG GTGCTGCAGT GCTTGCTGTG GGGGCCACGG 
CGGGAGCTGG TGGAGCAGCC GTGCACGTCA GAGGAGGTAC TGTGTAACAC TCTGCACAAC 
TGGATCGACA TGAAGCGAGC ACTGATGATG ATGAAGTTTG CTGAGAAGGC GGTGGACCGA 
AGGCGGGGTG TGGAACTGGA GGACTGGCTT TGCTGCCAGT ACTTGGCCTC GGCAGAGCCA 
GGGGCCCTCT TGCAGTCCCT GAAGCTCCTA CAACTTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766499.2
CDS	289..4410
Translation	MSACSDFVEHIWKPGSCKNCFCLRSDHQPTAGHPKARANSLPAGTRLPARPENCRLDDEGVNGLAYSKPTIAVKPTMMTSETSDLWTEASLSAEVPKVNWRRTPGKLLLPKQEDEPIVYLGSFRGLQKPASPLACTDGNSRCPPAYTMVGLHNLEARVDRNTAFQPVSFQEEKAGREELPSAHESFRQKLAAFAGMTSSCPKGPRPCTSPQPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPESKDAVHSTEGSGRRGGDCSPTCREQGPRTRPTEEEKQGLSFPRECCGQGSTANPPRLGPKKPSLNSEAASSSDGLSCGSSRSGASSPFAPHLENDYCSLVKEPASGKQQDLSGHFLTSGKCVGQAAELQPASLLRDPVQPEPIYAESAKRKKAAPGPPRPEPKKEQVPAGHSQGQVWTGDTWIQKTPPSWSQDREGANPAPQVATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGPSNQDLQAGEEEPLVAQGLTSRESHPHNVTENTAKEKPAIPPKLSKSSPGGSPVSPAPPLTDHSDGNTGGSSVGPQLLSRVPANLTSSCHTNGVATAGDSAKCPPPATSSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELSQSWGRELENGTADHSNSSTWHRLHPIDGTSGQNSKTNSGMSKSASFAFEFPKDRGRLEAFSPPPPPPKSRHLLKMNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRPCSDGGPSYEPTHSPTISGKKLFAPVPFPSGSTEDVSPGGGPAQPPPLPQKKIVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPFNCSLNSGNRSHHVFSSSEPLGKAFKGNAPWAPALGLANSKGGCGSPSLQCRAATSTSSSQLSVSSQASSSSTQLQLHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGQKKELRFNENYWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVASVPSSMLASPDTSSKDTAPAVSPQPPAQEQDCVVVITREVPHQTASDFVRDSMASHRAEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLAHCNPQSSPGPSATPTVPTTTSRCPSAAPAATTACQGGPGEKQLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTLSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCTSEEVLCNTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	NM_172911.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PEAK1 related kinase activating pseudokinase 1 (Prag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172911.3

ORF Insert Sequence:

ATGTCTGCGT GCAGCGACTT TGTGGAGCAC ATCTGGAAAC CCGGGTCTTG CAAGAACTGC 
TTCTGCTTGC GAAGTGATCA TCAGCCAACA GCTGGCCATC CCAAGGCTAG AGCCAACAGC 
CTACCCGCTG GAACTCGCCT GCCTGCCAGG CCTGAGAATT GCCGCCTAGA CGACGAAGGC 
GTGAATGGCT TGGCCTACTC AAAGCCCACC ATTGCTGTAA AGCCCACCAT GATGACTTCT 
GAGACCTCCG ACTTGTGGAC TGAAGCCAGC CTGAGCGCTG AAGTCCCAAA GGTCAATTGG 
AGACGAACCC CAGGCAAGCT CCTGCTCCCG AAACAGGAAG ATGAGCCAAT AGTTTACCTA 
GGCAGCTTCC GAGGACTGCA GAAGCCTGCA AGCCCTCTTG CCTGCACAGA TGGCAACTCT 
CGTTGTCCCC CAGCCTATAC CATGGTCGGA CTGCATAACC TGGAGGCCCG CGTGGATAGG 
AACACTGCCT TTCAACCAGT GAGCTTCCAG GAAGAGAAGG CTGGGAGGGA AGAGCTGCCC 
TCAGCTCACG AGAGCTTTCG CCAGAAGCTG GCTGCCTTTG CAGGGATGAC ATCCAGCTGC 
CCCAAGGGCC CCAGGCCCTG CACTTCTCCA CAGCCCCTGC GGGAATCCCT GCCCTCGGAG 
GATGACAGTG ACCAAAGGTG CTCACCCTCA GGAGACAGCG AAGGTGGGGA GTACTGTTCC 
ATTCTGGACT GTTGTCCAGA ATCCAAAGAT GCTGTGCATA GCACCGAGGG CTCTGGCAGA 
CGTGGAGGGG ACTGCTCACC CACGTGCAGG GAGCAGGGAC CACGTACAAG ACCAACAGAA 
GAGGAAAAAC AGGGTCTGAG CTTCCCCAGA GAGTGCTGTG GTCAGGGATC CACAGCGAAC 
CCGCCCCGCC TGGGCCCCAA GAAGCCATCC CTTAACTCTG AGGCCGCCAG CTCTTCCGAT 
GGTCTGTCCT GTGGCAGCAG CCGCAGCGGG GCCAGCAGCC CCTTCGCTCC CCACCTTGAG 
AACGACTACT GCTCTCTTGT GAAGGAACCG GCCTCAGGGA AGCAGCAAGA CCTCAGCGGC 
CACTTTCTCA CCTCTGGCAA ATGTGTGGGT CAAGCTGCGG AACTCCAGCC CGCCTCCCTT 
CTTAGGGACC CTGTGCAGCC TGAACCCATC TACGCCGAAA GCGCCAAGAG GAAGAAGGCA 
GCTCCGGGGC CTCCGAGGCC CGAGCCCAAG AAAGAGCAGG TCCCAGCTGG CCATAGCCAG 
GGCCAAGTAT GGACAGGTGA TACCTGGATT CAGAAGACAC CCCCTAGCTG GAGCCAGGAC 
AGGGAAGGCG CAAACCCTGC TCCTCAGGTG GCAACCACCA TCACTGTCAT CGCTGCTCAC 
CCGGAAGAGG ACCACAGGAC TATTTATCTG AGCAGCCCTG ACTCTGCCGT GGGGGTGCAG 
TGGCCACGTG GGCCTTCGAA CCAGGACTTA CAAGCCGGTG AAGAGGAGCC TTTGGTTGCG 
CAGGGGCTAA CCTCAAGGGA GAGCCATCCA CATAATGTGA CTGAAAACAC GGCCAAAGAG 
AAGCCTGCCA TTCCCCCCAA ACTGTCCAAA AGCAGCCCCG GAGGGTCCCC TGTGTCTCCC 
GCGCCCCCAC TGACTGACCA CAGCGACGGG AACACCGGTG GCAGCAGCGT CGGACCCCAA 
CTTTTGTCTA GAGTCCCTGC TAACCTAACG TCTTCCTGCC ACACCAATGG CGTTGCCACT 
GCTGGTGACT CTGCCAAGTG TCCCCCACCA GCCACCTCCT CTTCAGTCTT GGACCAGAGG 
CGGCCAAGAT ACCAGACTGG TGCTTGGAGC CGCCAGTGCC GGATAGAAGA GGAGGAGGAG 
GTGGGGCAGG AGTTGAGTCA AAGTTGGGGG AGAGAGTTGG AAAACGGTAC CGCAGACCAT 
TCAAACTCCT CTACCTGGCA CCGTCTCCAT CCCATCGATG GCACCTCTGG GCAGAACAGC 
AAAACTAACT CTGGGATGAG CAAATCGGCT TCCTTTGCGT TTGAGTTCCC AAAGGACAGA 
GGCAGATTGG AGGCATTCTC ACCCCCTCCC CCACCTCCAA AGTCAAGGCA CCTGTTAAAA 
ATGAACAAGA GCAGTTCTGA CTTGGAAAAA GTGAGCCAGA GTTCTGCAGA AAGCCTCAGC 
CCGTCCTTCA GAGGGGCCCA CGTCAGCTTC ACCACGGGCT CCACAGATAG CCTAGCCTCG 
GACTCCAGGC CCTGCAGCGA TGGAGGTCCA TCATATGAAC CCACCCACTC ACCCACCATC 
AGCGGGAAAA AGCTCTTCGC TCCCGTCCCT TTTCCATCAG GCTCCACAGA GGATGTGTCC 
CCTGGTGGTG GTCCTGCGCA GCCTCCTCCT CTGCCCCAGA AAAAGATAGT GAGCCGTGCC 
GCCTCTTCCC CTGATGGCTT CTTCTGGACC CAAGGTTCCC CTAAGCCACG GACGGCAAGT 
CCCAAGCTGA ACCTAAGCCA TTCAGAAACC AATGTTTGTG CCCATGACGA GCCTCCTTTT 
AACTGTTCCT TGAACTCTGG AAACCGTTCC CACCATGTCT TCTCCTCTTC TGAGCCTTTG 
GGGAAAGCTT TCAAAGGCAA TGCTCCCTGG GCCCCAGCTC TGGGGCTGGC GAACAGCAAA 
GGCGGCTGTG GGAGCCCCAG TCTCCAGTGC AGAGCGGCCA CCTCCACGTC ATCATCCCAG 
CTCAGCGTGT CCAGCCAGGC CTCCTCCAGC AGCACCCAGC TTCAGCTGCA CAGCCTGCTG 
AGTAGCATCA GCAGCAAGGA GGGCACCTAT GCCAAGCTTG GGGGCCTCTA CACACAATCC 
CTGGCCCGCC TAGTGACCAA GTGTGAAGAT CTCTTCATGG GTGGCCAGAA AAAGGAACTG 
CGCTTCAATG AGAATTACTG GTCACTCTTC AAGCTGACGT GCAACAAACC TTGCTGTGAC 
TCGGGGGATG CAATTTACTA CTGTGCCACC TGCTCTGAGG ACCCAGGCAG CATCTATGCT 
GTGAAAATCT GCAAAACCCC AGAACCCAAA TCAGCCTCAT ACTGCAGCCC ATCTGTGCCG 
GTGCATTTCA ACATCCAGCA AGACTGCGGC CACTTTGTGG CCTCTGTGCC CTCCAGCATG 
CTTGCCTCCC CTGACACATC CAGCAAAGAC ACCGCGCCTG CCGTATCCCC ACAGCCCCCT 
GCCCAGGAGC AGGACTGTGT GGTGGTCATC ACCCGGGAGG TACCGCACCA GACTGCCTCC 
GACTTTGTGC GGGACTCTAT GGCCAGCCAC CGGGCGGAGC CTGAGGTTTA CGAGCGCCGG 
GTGTGCTTCC TGCTTCTGCA GCTCTGCAAC GGGCTGGAGC ACCTGAAGGA ACACGGGATC 
ATCCACCGGG ACCTGTGTCT GGAGAACCTG CTGCTGGCGC ACTGTAACCC TCAGAGCTCC 
CCCGGGCCCT CTGCCACTCC TACCGTGCCC ACCACCACAT CCAGGTGCCC TTCTGCCGCG 
CCCGCAGCCA CCACTGCCTG CCAGGGAGGG CCTGGTGAGA AGCAGCTGCC CAGGCTCATC 
ATCAGCAACT TCCTGAAGGC CAAGCAAAAG CCAGGAGGCA CCACCAACCT GCAACAGAAG 
AAGAGCCAGG CCCGGCTGGC TCCCGAGATC GTGTCTGCCT CCCAGTACCG CAAGTTTGAT 
GAGTTCCAGA CGGGCATCCT CATCTATGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGAGCCCAGC TCCGGGAGCG CGACTACCGG CGGGAGGACT TACCCCCGCT GCCCACACTG 
TCCCTCTACT CTCCCGGCCT GCAGCAACTC GCCCATCTAC TGCTTGAGGC TGATCCCATC 
AAGCGCATCC GCATAGGTGA AGCTAAGCGG GTGCTGCAGT GCTTGCTGTG GGGGCCACGG 
CGGGAGCTGG TGGAGCAGCC GTGCACGTCA GAGGAGGTAC TGTGTAACAC TCTGCACAAC 
TGGATCGACA TGAAGCGAGC ACTGATGATG ATGAAGTTTG CTGAGAAGGC GGTGGACCGA 
AGGCGGGGTG TGGAACTGGA GGACTGGCTT TGCTGCCAGT ACTTGGCCTC GGCAGAGCCA 
GGGGCCCTCT TGCAGTCCCT GAAGCTCCTA CAACTTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prag1 ( XM_011242137.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240439.1 Mus musculus DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21183
Clone ID Related Accession (Same CDS sequence)	XM_011242137.1 , NM_172911.3 , XM_011242137.2
Accession Version	XM_011242137.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGT GCAGCGACTT TGTGGAGCAC ATCTGGAAAC CCGGGTCTTG CAAGAACTGC 
TTCTGCTTGC GAAGTGATCA TCAGCCAACA GCTGGCCATC CCAAGGCTAG AGCCAACAGC 
CTACCCGCTG GAACTCGCCT GCCTGCCAGG CCTGAGAATT GCCGCCTAGA CGACGAAGGC 
GTGAATGGCT TGGCCTACTC AAAGCCCACC ATTGCTGTAA AGCCCACCAT GATGACTTCT 
GAGACCTCCG ACTTGTGGAC TGAAGCCAGC CTGAGCGCTG AAGTCCCAAA GGTCAATTGG 
AGACGAACCC CAGGCAAGCT CCTGCTCCCG AAACAGGAAG ATGAGCCAAT AGTTTACCTA 
GGCAGCTTCC GAGGACTGCA GAAGCCTGCA AGCCCTCTTG CCTGCACAGA TGGCAACTCT 
CGTTGTCCCC CAGCCTATAC CATGGTCGGA CTGCATAACC TGGAGGCCCG CGTGGATAGG 
AACACTGCCT TTCAACCAGT GAGCTTCCAG GAAGAGAAGG CTGGGAGGGA AGAGCTGCCC 
TCAGCTCACG AGAGCTTTCG CCAGAAGCTG GCTGCCTTTG CAGGGATGAC ATCCAGCTGC 
CCCAAGGGCC CCAGGCCCTG CACTTCTCCA CAGCCCCTGC GGGAATCCCT GCCCTCGGAG 
GATGACAGTG ACCAAAGGTG CTCACCCTCA GGAGACAGCG AAGGTGGGGA GTACTGTTCC 
ATTCTGGACT GTTGTCCAGA ATCCAAAGAT GCTGTGCATA GCACCGAGGG CTCTGGCAGA 
CGTGGAGGGG ACTGCTCACC CACGTGCAGG GAGCAGGGAC CACGTACAAG ACCAACAGAA 
GAGGAAAAAC AGGGTCTGAG CTTCCCCAGA GAGTGCTGTG GTCAGGGATC CACAGCGAAC 
CCGCCCCGCC TGGGCCCCAA GAAGCCATCC CTTAACTCTG AGGCCGCCAG CTCTTCCGAT 
GGTCTGTCCT GTGGCAGCAG CCGCAGCGGG GCCAGCAGCC CCTTCGCTCC CCACCTTGAG 
AACGACTACT GCTCTCTTGT GAAGGAACCG GCCTCAGGGA AGCAGCAAGA CCTCAGCGGC 
CACTTTCTCA CCTCTGGCAA ATGTGTGGGT CAAGCTGCGG AACTCCAGCC CGCCTCCCTT 
CTTAGGGACC CTGTGCAGCC TGAACCCATC TACGCCGAAA GCGCCAAGAG GAAGAAGGCA 
GCTCCGGGGC CTCCGAGGCC CGAGCCCAAG AAAGAGCAGG TCCCAGCTGG CCATAGCCAG 
GGCCAAGTAT GGACAGGTGA TACCTGGATT CAGAAGACAC CCCCTAGCTG GAGCCAGGAC 
AGGGAAGGCG CAAACCCTGC TCCTCAGGTG GCAACCACCA TCACTGTCAT CGCTGCTCAC 
CCGGAAGAGG ACCACAGGAC TATTTATCTG AGCAGCCCTG ACTCTGCCGT GGGGGTGCAG 
TGGCCACGTG GGCCTTCGAA CCAGGACTTA CAAGCCGGTG AAGAGGAGCC TTTGGTTGCG 
CAGGGGCTAA CCTCAAGGGA GAGCCATCCA CATAATGTGA CTGAAAACAC GGCCAAAGAG 
AAGCCTGCCA TTCCCCCCAA ACTGTCCAAA AGCAGCCCCG GAGGGTCCCC TGTGTCTCCC 
GCGCCCCCAC TGACTGACCA CAGCGACGGG AACACCGGTG GCAGCAGCGT CGGACCCCAA 
CTTTTGTCTA GAGTCCCTGC TAACCTAACG TCTTCCTGCC ACACCAATGG CGTTGCCACT 
GCTGGTGACT CTGCCAAGTG TCCCCCACCA GCCACCTCCT CTTCAGTCTT GGACCAGAGG 
CGGCCAAGAT ACCAGACTGG TGCTTGGAGC CGCCAGTGCC GGATAGAAGA GGAGGAGGAG 
GTGGGGCAGG AGTTGAGTCA AAGTTGGGGG AGAGAGTTGG AAAACGGTAC CGCAGACCAT 
TCAAACTCCT CTACCTGGCA CCGTCTCCAT CCCATCGATG GCACCTCTGG GCAGAACAGC 
AAAACTAACT CTGGGATGAG CAAATCGGCT TCCTTTGCGT TTGAGTTCCC AAAGGACAGA 
GGCAGATTGG AGGCATTCTC ACCCCCTCCC CCACCTCCAA AGTCAAGGCA CCTGTTAAAA 
ATGAACAAGA GCAGTTCTGA CTTGGAAAAA GTGAGCCAGA GTTCTGCAGA AAGCCTCAGC 
CCGTCCTTCA GAGGGGCCCA CGTCAGCTTC ACCACGGGCT CCACAGATAG CCTAGCCTCG 
GACTCCAGGC CCTGCAGCGA TGGAGGTCCA TCATATGAAC CCACCCACTC ACCCACCATC 
AGCGGGAAAA AGCTCTTCGC TCCCGTCCCT TTTCCATCAG GCTCCACAGA GGATGTGTCC 
CCTGGTGGTG GTCCTGCGCA GCCTCCTCCT CTGCCCCAGA AAAAGATAGT GAGCCGTGCC 
GCCTCTTCCC CTGATGGCTT CTTCTGGACC CAAGGTTCCC CTAAGCCACG GACGGCAAGT 
CCCAAGCTGA ACCTAAGCCA TTCAGAAACC AATGTTTGTG CCCATGACGA GCCTCCTTTT 
AACTGTTCCT TGAACTCTGG AAACCGTTCC CACCATGTCT TCTCCTCTTC TGAGCCTTTG 
GGGAAAGCTT TCAAAGGCAA TGCTCCCTGG GCCCCAGCTC TGGGGCTGGC GAACAGCAAA 
GGCGGCTGTG GGAGCCCCAG TCTCCAGTGC AGAGCGGCCA CCTCCACGTC ATCATCCCAG 
CTCAGCGTGT CCAGCCAGGC CTCCTCCAGC AGCACCCAGC TTCAGCTGCA CAGCCTGCTG 
AGTAGCATCA GCAGCAAGGA GGGCACCTAT GCCAAGCTTG GGGGCCTCTA CACACAATCC 
CTGGCCCGCC TAGTGACCAA GTGTGAAGAT CTCTTCATGG GTGGCCAGAA AAAGGAACTG 
CGCTTCAATG AGAATTACTG GTCACTCTTC AAGCTGACGT GCAACAAACC TTGCTGTGAC 
TCGGGGGATG CAATTTACTA CTGTGCCACC TGCTCTGAGG ACCCAGGCAG CATCTATGCT 
GTGAAAATCT GCAAAACCCC AGAACCCAAA TCAGCCTCAT ACTGCAGCCC ATCTGTGCCG 
GTGCATTTCA ACATCCAGCA AGACTGCGGC CACTTTGTGG CCTCTGTGCC CTCCAGCATG 
CTTGCCTCCC CTGACACATC CAGCAAAGAC ACCGCGCCTG CCGTATCCCC ACAGCCCCCT 
GCCCAGGAGC AGGACTGTGT GGTGGTCATC ACCCGGGAGG TACCGCACCA GACTGCCTCC 
GACTTTGTGC GGGACTCTAT GGCCAGCCAC CGGGCGGAGC CTGAGGTTTA CGAGCGCCGG 
GTGTGCTTCC TGCTTCTGCA GCTCTGCAAC GGGCTGGAGC ACCTGAAGGA ACACGGGATC 
ATCCACCGGG ACCTGTGTCT GGAGAACCTG CTGCTGGCGC ACTGTAACCC TCAGAGCTCC 
CCCGGGCCCT CTGCCACTCC TACCGTGCCC ACCACCACAT CCAGGTGCCC TTCTGCCGCG 
CCCGCAGCCA CCACTGCCTG CCAGGGAGGG CCTGGTGAGA AGCAGCTGCC CAGGCTCATC 
ATCAGCAACT TCCTGAAGGC CAAGCAAAAG CCAGGAGGCA CCACCAACCT GCAACAGAAG 
AAGAGCCAGG CCCGGCTGGC TCCCGAGATC GTGTCTGCCT CCCAGTACCG CAAGTTTGAT 
GAGTTCCAGA CGGGCATCCT CATCTATGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGAGCCCAGC TCCGGGAGCG CGACTACCGG CGGGAGGACT TACCCCCGCT GCCCACACTG 
TCCCTCTACT CTCCCGGCCT GCAGCAACTC GCCCATCTAC TGCTTGAGGC TGATCCCATC 
AAGCGCATCC GCATAGGTGA AGCTAAGCGG GTGCTGCAGT GCTTGCTGTG GGGGCCACGG 
CGGGAGCTGG TGGAGCAGCC GTGCACGTCA GAGGAGGTAC TGTGTAACAC TCTGCACAAC 
TGGATCGACA TGAAGCGAGC ACTGATGATG ATGAAGTTTG CTGAGAAGGC GGTGGACCGA 
AGGCGGGGTG TGGAACTGGA GGACTGGCTT TGCTGCCAGT ACTTGGCCTC GGCAGAGCCA 
GGGGCCCTCT TGCAGTCCCT GAAGCTCCTA CAACTTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240439.1
CDS	511..4632
Translation	MSACSDFVEHIWKPGSCKNCFCLRSDHQPTAGHPKARANSLPAGTRLPARPENCRLDDEGVNGLAYSKPTIAVKPTMMTSETSDLWTEASLSAEVPKVNWRRTPGKLLLPKQEDEPIVYLGSFRGLQKPASPLACTDGNSRCPPAYTMVGLHNLEARVDRNTAFQPVSFQEEKAGREELPSAHESFRQKLAAFAGMTSSCPKGPRPCTSPQPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPESKDAVHSTEGSGRRGGDCSPTCREQGPRTRPTEEEKQGLSFPRECCGQGSTANPPRLGPKKPSLNSEAASSSDGLSCGSSRSGASSPFAPHLENDYCSLVKEPASGKQQDLSGHFLTSGKCVGQAAELQPASLLRDPVQPEPIYAESAKRKKAAPGPPRPEPKKEQVPAGHSQGQVWTGDTWIQKTPPSWSQDREGANPAPQVATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGPSNQDLQAGEEEPLVAQGLTSRESHPHNVTENTAKEKPAIPPKLSKSSPGGSPVSPAPPLTDHSDGNTGGSSVGPQLLSRVPANLTSSCHTNGVATAGDSAKCPPPATSSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELSQSWGRELENGTADHSNSSTWHRLHPIDGTSGQNSKTNSGMSKSASFAFEFPKDRGRLEAFSPPPPPPKSRHLLKMNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRPCSDGGPSYEPTHSPTISGKKLFAPVPFPSGSTEDVSPGGGPAQPPPLPQKKIVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPFNCSLNSGNRSHHVFSSSEPLGKAFKGNAPWAPALGLANSKGGCGSPSLQCRAATSTSSSQLSVSSQASSSSTQLQLHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGQKKELRFNENYWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVASVPSSMLASPDTSSKDTAPAVSPQPPAQEQDCVVVITREVPHQTASDFVRDSMASHRAEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLAHCNPQSSPGPSATPTVPTTTSRCPSAAPAATTACQGGPGEKQLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTLSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCTSEEVLCNTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_011242137.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242137.1

ORF Insert Sequence:

ATGTCTGCGT GCAGCGACTT TGTGGAGCAC ATCTGGAAAC CCGGGTCTTG CAAGAACTGC 
TTCTGCTTGC GAAGTGATCA TCAGCCAACA GCTGGCCATC CCAAGGCTAG AGCCAACAGC 
CTACCCGCTG GAACTCGCCT GCCTGCCAGG CCTGAGAATT GCCGCCTAGA CGACGAAGGC 
GTGAATGGCT TGGCCTACTC AAAGCCCACC ATTGCTGTAA AGCCCACCAT GATGACTTCT 
GAGACCTCCG ACTTGTGGAC TGAAGCCAGC CTGAGCGCTG AAGTCCCAAA GGTCAATTGG 
AGACGAACCC CAGGCAAGCT CCTGCTCCCG AAACAGGAAG ATGAGCCAAT AGTTTACCTA 
GGCAGCTTCC GAGGACTGCA GAAGCCTGCA AGCCCTCTTG CCTGCACAGA TGGCAACTCT 
CGTTGTCCCC CAGCCTATAC CATGGTCGGA CTGCATAACC TGGAGGCCCG CGTGGATAGG 
AACACTGCCT TTCAACCAGT GAGCTTCCAG GAAGAGAAGG CTGGGAGGGA AGAGCTGCCC 
TCAGCTCACG AGAGCTTTCG CCAGAAGCTG GCTGCCTTTG CAGGGATGAC ATCCAGCTGC 
CCCAAGGGCC CCAGGCCCTG CACTTCTCCA CAGCCCCTGC GGGAATCCCT GCCCTCGGAG 
GATGACAGTG ACCAAAGGTG CTCACCCTCA GGAGACAGCG AAGGTGGGGA GTACTGTTCC 
ATTCTGGACT GTTGTCCAGA ATCCAAAGAT GCTGTGCATA GCACCGAGGG CTCTGGCAGA 
CGTGGAGGGG ACTGCTCACC CACGTGCAGG GAGCAGGGAC CACGTACAAG ACCAACAGAA 
GAGGAAAAAC AGGGTCTGAG CTTCCCCAGA GAGTGCTGTG GTCAGGGATC CACAGCGAAC 
CCGCCCCGCC TGGGCCCCAA GAAGCCATCC CTTAACTCTG AGGCCGCCAG CTCTTCCGAT 
GGTCTGTCCT GTGGCAGCAG CCGCAGCGGG GCCAGCAGCC CCTTCGCTCC CCACCTTGAG 
AACGACTACT GCTCTCTTGT GAAGGAACCG GCCTCAGGGA AGCAGCAAGA CCTCAGCGGC 
CACTTTCTCA CCTCTGGCAA ATGTGTGGGT CAAGCTGCGG AACTCCAGCC CGCCTCCCTT 
CTTAGGGACC CTGTGCAGCC TGAACCCATC TACGCCGAAA GCGCCAAGAG GAAGAAGGCA 
GCTCCGGGGC CTCCGAGGCC CGAGCCCAAG AAAGAGCAGG TCCCAGCTGG CCATAGCCAG 
GGCCAAGTAT GGACAGGTGA TACCTGGATT CAGAAGACAC CCCCTAGCTG GAGCCAGGAC 
AGGGAAGGCG CAAACCCTGC TCCTCAGGTG GCAACCACCA TCACTGTCAT CGCTGCTCAC 
CCGGAAGAGG ACCACAGGAC TATTTATCTG AGCAGCCCTG ACTCTGCCGT GGGGGTGCAG 
TGGCCACGTG GGCCTTCGAA CCAGGACTTA CAAGCCGGTG AAGAGGAGCC TTTGGTTGCG 
CAGGGGCTAA CCTCAAGGGA GAGCCATCCA CATAATGTGA CTGAAAACAC GGCCAAAGAG 
AAGCCTGCCA TTCCCCCCAA ACTGTCCAAA AGCAGCCCCG GAGGGTCCCC TGTGTCTCCC 
GCGCCCCCAC TGACTGACCA CAGCGACGGG AACACCGGTG GCAGCAGCGT CGGACCCCAA 
CTTTTGTCTA GAGTCCCTGC TAACCTAACG TCTTCCTGCC ACACCAATGG CGTTGCCACT 
GCTGGTGACT CTGCCAAGTG TCCCCCACCA GCCACCTCCT CTTCAGTCTT GGACCAGAGG 
CGGCCAAGAT ACCAGACTGG TGCTTGGAGC CGCCAGTGCC GGATAGAAGA GGAGGAGGAG 
GTGGGGCAGG AGTTGAGTCA AAGTTGGGGG AGAGAGTTGG AAAACGGTAC CGCAGACCAT 
TCAAACTCCT CTACCTGGCA CCGTCTCCAT CCCATCGATG GCACCTCTGG GCAGAACAGC 
AAAACTAACT CTGGGATGAG CAAATCGGCT TCCTTTGCGT TTGAGTTCCC AAAGGACAGA 
GGCAGATTGG AGGCATTCTC ACCCCCTCCC CCACCTCCAA AGTCAAGGCA CCTGTTAAAA 
ATGAACAAGA GCAGTTCTGA CTTGGAAAAA GTGAGCCAGA GTTCTGCAGA AAGCCTCAGC 
CCGTCCTTCA GAGGGGCCCA CGTCAGCTTC ACCACGGGCT CCACAGATAG CCTAGCCTCG 
GACTCCAGGC CCTGCAGCGA TGGAGGTCCA TCATATGAAC CCACCCACTC ACCCACCATC 
AGCGGGAAAA AGCTCTTCGC TCCCGTCCCT TTTCCATCAG GCTCCACAGA GGATGTGTCC 
CCTGGTGGTG GTCCTGCGCA GCCTCCTCCT CTGCCCCAGA AAAAGATAGT GAGCCGTGCC 
GCCTCTTCCC CTGATGGCTT CTTCTGGACC CAAGGTTCCC CTAAGCCACG GACGGCAAGT 
CCCAAGCTGA ACCTAAGCCA TTCAGAAACC AATGTTTGTG CCCATGACGA GCCTCCTTTT 
AACTGTTCCT TGAACTCTGG AAACCGTTCC CACCATGTCT TCTCCTCTTC TGAGCCTTTG 
GGGAAAGCTT TCAAAGGCAA TGCTCCCTGG GCCCCAGCTC TGGGGCTGGC GAACAGCAAA 
GGCGGCTGTG GGAGCCCCAG TCTCCAGTGC AGAGCGGCCA CCTCCACGTC ATCATCCCAG 
CTCAGCGTGT CCAGCCAGGC CTCCTCCAGC AGCACCCAGC TTCAGCTGCA CAGCCTGCTG 
AGTAGCATCA GCAGCAAGGA GGGCACCTAT GCCAAGCTTG GGGGCCTCTA CACACAATCC 
CTGGCCCGCC TAGTGACCAA GTGTGAAGAT CTCTTCATGG GTGGCCAGAA AAAGGAACTG 
CGCTTCAATG AGAATTACTG GTCACTCTTC AAGCTGACGT GCAACAAACC TTGCTGTGAC 
TCGGGGGATG CAATTTACTA CTGTGCCACC TGCTCTGAGG ACCCAGGCAG CATCTATGCT 
GTGAAAATCT GCAAAACCCC AGAACCCAAA TCAGCCTCAT ACTGCAGCCC ATCTGTGCCG 
GTGCATTTCA ACATCCAGCA AGACTGCGGC CACTTTGTGG CCTCTGTGCC CTCCAGCATG 
CTTGCCTCCC CTGACACATC CAGCAAAGAC ACCGCGCCTG CCGTATCCCC ACAGCCCCCT 
GCCCAGGAGC AGGACTGTGT GGTGGTCATC ACCCGGGAGG TACCGCACCA GACTGCCTCC 
GACTTTGTGC GGGACTCTAT GGCCAGCCAC CGGGCGGAGC CTGAGGTTTA CGAGCGCCGG 
GTGTGCTTCC TGCTTCTGCA GCTCTGCAAC GGGCTGGAGC ACCTGAAGGA ACACGGGATC 
ATCCACCGGG ACCTGTGTCT GGAGAACCTG CTGCTGGCGC ACTGTAACCC TCAGAGCTCC 
CCCGGGCCCT CTGCCACTCC TACCGTGCCC ACCACCACAT CCAGGTGCCC TTCTGCCGCG 
CCCGCAGCCA CCACTGCCTG CCAGGGAGGG CCTGGTGAGA AGCAGCTGCC CAGGCTCATC 
ATCAGCAACT TCCTGAAGGC CAAGCAAAAG CCAGGAGGCA CCACCAACCT GCAACAGAAG 
AAGAGCCAGG CCCGGCTGGC TCCCGAGATC GTGTCTGCCT CCCAGTACCG CAAGTTTGAT 
GAGTTCCAGA CGGGCATCCT CATCTATGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGAGCCCAGC TCCGGGAGCG CGACTACCGG CGGGAGGACT TACCCCCGCT GCCCACACTG 
TCCCTCTACT CTCCCGGCCT GCAGCAACTC GCCCATCTAC TGCTTGAGGC TGATCCCATC 
AAGCGCATCC GCATAGGTGA AGCTAAGCGG GTGCTGCAGT GCTTGCTGTG GGGGCCACGG 
CGGGAGCTGG TGGAGCAGCC GTGCACGTCA GAGGAGGTAC TGTGTAACAC TCTGCACAAC 
TGGATCGACA TGAAGCGAGC ACTGATGATG ATGAAGTTTG CTGAGAAGGC GGTGGACCGA 
AGGCGGGGTG TGGAACTGGA GGACTGGCTT TGCTGCCAGT ACTTGGCCTC GGCAGAGCCA 
GGGGCCCTCT TGCAGTCCCT GAAGCTCCTA CAACTTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prag1 ( XM_011242137.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240439.1 Mus musculus PEAK1 related kinase activating pseudokinase 1 (Prag1), transcript variant X1, mRNA.

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CloneID	OMu21183
Clone ID Related Accession (Same CDS sequence)	XM_011242137.1 , NM_172911.3 , XM_011242137.2
Accession Version	XM_011242137.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	inactive tyrosine-protein kinase PRAG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGT GCAGCGACTT TGTGGAGCAC ATCTGGAAAC CCGGGTCTTG CAAGAACTGC 
TTCTGCTTGC GAAGTGATCA TCAGCCAACA GCTGGCCATC CCAAGGCTAG AGCCAACAGC 
CTACCCGCTG GAACTCGCCT GCCTGCCAGG CCTGAGAATT GCCGCCTAGA CGACGAAGGC 
GTGAATGGCT TGGCCTACTC AAAGCCCACC ATTGCTGTAA AGCCCACCAT GATGACTTCT 
GAGACCTCCG ACTTGTGGAC TGAAGCCAGC CTGAGCGCTG AAGTCCCAAA GGTCAATTGG 
AGACGAACCC CAGGCAAGCT CCTGCTCCCG AAACAGGAAG ATGAGCCAAT AGTTTACCTA 
GGCAGCTTCC GAGGACTGCA GAAGCCTGCA AGCCCTCTTG CCTGCACAGA TGGCAACTCT 
CGTTGTCCCC CAGCCTATAC CATGGTCGGA CTGCATAACC TGGAGGCCCG CGTGGATAGG 
AACACTGCCT TTCAACCAGT GAGCTTCCAG GAAGAGAAGG CTGGGAGGGA AGAGCTGCCC 
TCAGCTCACG AGAGCTTTCG CCAGAAGCTG GCTGCCTTTG CAGGGATGAC ATCCAGCTGC 
CCCAAGGGCC CCAGGCCCTG CACTTCTCCA CAGCCCCTGC GGGAATCCCT GCCCTCGGAG 
GATGACAGTG ACCAAAGGTG CTCACCCTCA GGAGACAGCG AAGGTGGGGA GTACTGTTCC 
ATTCTGGACT GTTGTCCAGA ATCCAAAGAT GCTGTGCATA GCACCGAGGG CTCTGGCAGA 
CGTGGAGGGG ACTGCTCACC CACGTGCAGG GAGCAGGGAC CACGTACAAG ACCAACAGAA 
GAGGAAAAAC AGGGTCTGAG CTTCCCCAGA GAGTGCTGTG GTCAGGGATC CACAGCGAAC 
CCGCCCCGCC TGGGCCCCAA GAAGCCATCC CTTAACTCTG AGGCCGCCAG CTCTTCCGAT 
GGTCTGTCCT GTGGCAGCAG CCGCAGCGGG GCCAGCAGCC CCTTCGCTCC CCACCTTGAG 
AACGACTACT GCTCTCTTGT GAAGGAACCG GCCTCAGGGA AGCAGCAAGA CCTCAGCGGC 
CACTTTCTCA CCTCTGGCAA ATGTGTGGGT CAAGCTGCGG AACTCCAGCC CGCCTCCCTT 
CTTAGGGACC CTGTGCAGCC TGAACCCATC TACGCCGAAA GCGCCAAGAG GAAGAAGGCA 
GCTCCGGGGC CTCCGAGGCC CGAGCCCAAG AAAGAGCAGG TCCCAGCTGG CCATAGCCAG 
GGCCAAGTAT GGACAGGTGA TACCTGGATT CAGAAGACAC CCCCTAGCTG GAGCCAGGAC 
AGGGAAGGCG CAAACCCTGC TCCTCAGGTG GCAACCACCA TCACTGTCAT CGCTGCTCAC 
CCGGAAGAGG ACCACAGGAC TATTTATCTG AGCAGCCCTG ACTCTGCCGT GGGGGTGCAG 
TGGCCACGTG GGCCTTCGAA CCAGGACTTA CAAGCCGGTG AAGAGGAGCC TTTGGTTGCG 
CAGGGGCTAA CCTCAAGGGA GAGCCATCCA CATAATGTGA CTGAAAACAC GGCCAAAGAG 
AAGCCTGCCA TTCCCCCCAA ACTGTCCAAA AGCAGCCCCG GAGGGTCCCC TGTGTCTCCC 
GCGCCCCCAC TGACTGACCA CAGCGACGGG AACACCGGTG GCAGCAGCGT CGGACCCCAA 
CTTTTGTCTA GAGTCCCTGC TAACCTAACG TCTTCCTGCC ACACCAATGG CGTTGCCACT 
GCTGGTGACT CTGCCAAGTG TCCCCCACCA GCCACCTCCT CTTCAGTCTT GGACCAGAGG 
CGGCCAAGAT ACCAGACTGG TGCTTGGAGC CGCCAGTGCC GGATAGAAGA GGAGGAGGAG 
GTGGGGCAGG AGTTGAGTCA AAGTTGGGGG AGAGAGTTGG AAAACGGTAC CGCAGACCAT 
TCAAACTCCT CTACCTGGCA CCGTCTCCAT CCCATCGATG GCACCTCTGG GCAGAACAGC 
AAAACTAACT CTGGGATGAG CAAATCGGCT TCCTTTGCGT TTGAGTTCCC AAAGGACAGA 
GGCAGATTGG AGGCATTCTC ACCCCCTCCC CCACCTCCAA AGTCAAGGCA CCTGTTAAAA 
ATGAACAAGA GCAGTTCTGA CTTGGAAAAA GTGAGCCAGA GTTCTGCAGA AAGCCTCAGC 
CCGTCCTTCA GAGGGGCCCA CGTCAGCTTC ACCACGGGCT CCACAGATAG CCTAGCCTCG 
GACTCCAGGC CCTGCAGCGA TGGAGGTCCA TCATATGAAC CCACCCACTC ACCCACCATC 
AGCGGGAAAA AGCTCTTCGC TCCCGTCCCT TTTCCATCAG GCTCCACAGA GGATGTGTCC 
CCTGGTGGTG GTCCTGCGCA GCCTCCTCCT CTGCCCCAGA AAAAGATAGT GAGCCGTGCC 
GCCTCTTCCC CTGATGGCTT CTTCTGGACC CAAGGTTCCC CTAAGCCACG GACGGCAAGT 
CCCAAGCTGA ACCTAAGCCA TTCAGAAACC AATGTTTGTG CCCATGACGA GCCTCCTTTT 
AACTGTTCCT TGAACTCTGG AAACCGTTCC CACCATGTCT TCTCCTCTTC TGAGCCTTTG 
GGGAAAGCTT TCAAAGGCAA TGCTCCCTGG GCCCCAGCTC TGGGGCTGGC GAACAGCAAA 
GGCGGCTGTG GGAGCCCCAG TCTCCAGTGC AGAGCGGCCA CCTCCACGTC ATCATCCCAG 
CTCAGCGTGT CCAGCCAGGC CTCCTCCAGC AGCACCCAGC TTCAGCTGCA CAGCCTGCTG 
AGTAGCATCA GCAGCAAGGA GGGCACCTAT GCCAAGCTTG GGGGCCTCTA CACACAATCC 
CTGGCCCGCC TAGTGACCAA GTGTGAAGAT CTCTTCATGG GTGGCCAGAA AAAGGAACTG 
CGCTTCAATG AGAATTACTG GTCACTCTTC AAGCTGACGT GCAACAAACC TTGCTGTGAC 
TCGGGGGATG CAATTTACTA CTGTGCCACC TGCTCTGAGG ACCCAGGCAG CATCTATGCT 
GTGAAAATCT GCAAAACCCC AGAACCCAAA TCAGCCTCAT ACTGCAGCCC ATCTGTGCCG 
GTGCATTTCA ACATCCAGCA AGACTGCGGC CACTTTGTGG CCTCTGTGCC CTCCAGCATG 
CTTGCCTCCC CTGACACATC CAGCAAAGAC ACCGCGCCTG CCGTATCCCC ACAGCCCCCT 
GCCCAGGAGC AGGACTGTGT GGTGGTCATC ACCCGGGAGG TACCGCACCA GACTGCCTCC 
GACTTTGTGC GGGACTCTAT GGCCAGCCAC CGGGCGGAGC CTGAGGTTTA CGAGCGCCGG 
GTGTGCTTCC TGCTTCTGCA GCTCTGCAAC GGGCTGGAGC ACCTGAAGGA ACACGGGATC 
ATCCACCGGG ACCTGTGTCT GGAGAACCTG CTGCTGGCGC ACTGTAACCC TCAGAGCTCC 
CCCGGGCCCT CTGCCACTCC TACCGTGCCC ACCACCACAT CCAGGTGCCC TTCTGCCGCG 
CCCGCAGCCA CCACTGCCTG CCAGGGAGGG CCTGGTGAGA AGCAGCTGCC CAGGCTCATC 
ATCAGCAACT TCCTGAAGGC CAAGCAAAAG CCAGGAGGCA CCACCAACCT GCAACAGAAG 
AAGAGCCAGG CCCGGCTGGC TCCCGAGATC GTGTCTGCCT CCCAGTACCG CAAGTTTGAT 
GAGTTCCAGA CGGGCATCCT CATCTATGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGAGCCCAGC TCCGGGAGCG CGACTACCGG CGGGAGGACT TACCCCCGCT GCCCACACTG 
TCCCTCTACT CTCCCGGCCT GCAGCAACTC GCCCATCTAC TGCTTGAGGC TGATCCCATC 
AAGCGCATCC GCATAGGTGA AGCTAAGCGG GTGCTGCAGT GCTTGCTGTG GGGGCCACGG 
CGGGAGCTGG TGGAGCAGCC GTGCACGTCA GAGGAGGTAC TGTGTAACAC TCTGCACAAC 
TGGATCGACA TGAAGCGAGC ACTGATGATG ATGAAGTTTG CTGAGAAGGC GGTGGACCGA 
AGGCGGGGTG TGGAACTGGA GGACTGGCTT TGCTGCCAGT ACTTGGCCTC GGCAGAGCCA 
GGGGCCCTCT TGCAGTCCCT GAAGCTCCTA CAACTTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240439.1
CDS	309..4430
Translation	MSACSDFVEHIWKPGSCKNCFCLRSDHQPTAGHPKARANSLPAGTRLPARPENCRLDDEGVNGLAYSKPTIAVKPTMMTSETSDLWTEASLSAEVPKVNWRRTPGKLLLPKQEDEPIVYLGSFRGLQKPASPLACTDGNSRCPPAYTMVGLHNLEARVDRNTAFQPVSFQEEKAGREELPSAHESFRQKLAAFAGMTSSCPKGPRPCTSPQPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPESKDAVHSTEGSGRRGGDCSPTCREQGPRTRPTEEEKQGLSFPRECCGQGSTANPPRLGPKKPSLNSEAASSSDGLSCGSSRSGASSPFAPHLENDYCSLVKEPASGKQQDLSGHFLTSGKCVGQAAELQPASLLRDPVQPEPIYAESAKRKKAAPGPPRPEPKKEQVPAGHSQGQVWTGDTWIQKTPPSWSQDREGANPAPQVATTITVIAAHPEEDHRTIYLSSPDSAVGVQWPRGPSNQDLQAGEEEPLVAQGLTSRESHPHNVTENTAKEKPAIPPKLSKSSPGGSPVSPAPPLTDHSDGNTGGSSVGPQLLSRVPANLTSSCHTNGVATAGDSAKCPPPATSSSVLDQRRPRYQTGAWSRQCRIEEEEEVGQELSQSWGRELENGTADHSNSSTWHRLHPIDGTSGQNSKTNSGMSKSASFAFEFPKDRGRLEAFSPPPPPPKSRHLLKMNKSSSDLEKVSQSSAESLSPSFRGAHVSFTTGSTDSLASDSRPCSDGGPSYEPTHSPTISGKKLFAPVPFPSGSTEDVSPGGGPAQPPPLPQKKIVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHDEPPFNCSLNSGNRSHHVFSSSEPLGKAFKGNAPWAPALGLANSKGGCGSPSLQCRAATSTSSSQLSVSSQASSSSTQLQLHSLLSSISSKEGTYAKLGGLYTQSLARLVTKCEDLFMGGQKKELRFNENYWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSIYAVKICKTPEPKSASYCSPSVPVHFNIQQDCGHFVASVPSSMLASPDTSSKDTAPAVSPQPPAQEQDCVVVITREVPHQTASDFVRDSMASHRAEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLAHCNPQSSPGPSATPTVPTTTSRCPSAAPAATTACQGGPGEKQLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRREDLPPLPTLSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEQPCTSEEVLCNTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_011242137.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PEAK1 related kinase activating pseudokinase 1 (Prag1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242137.2

ORF Insert Sequence:

ATGTCTGCGT GCAGCGACTT TGTGGAGCAC ATCTGGAAAC CCGGGTCTTG CAAGAACTGC 
TTCTGCTTGC GAAGTGATCA TCAGCCAACA GCTGGCCATC CCAAGGCTAG AGCCAACAGC 
CTACCCGCTG GAACTCGCCT GCCTGCCAGG CCTGAGAATT GCCGCCTAGA CGACGAAGGC 
GTGAATGGCT TGGCCTACTC AAAGCCCACC ATTGCTGTAA AGCCCACCAT GATGACTTCT 
GAGACCTCCG ACTTGTGGAC TGAAGCCAGC CTGAGCGCTG AAGTCCCAAA GGTCAATTGG 
AGACGAACCC CAGGCAAGCT CCTGCTCCCG AAACAGGAAG ATGAGCCAAT AGTTTACCTA 
GGCAGCTTCC GAGGACTGCA GAAGCCTGCA AGCCCTCTTG CCTGCACAGA TGGCAACTCT 
CGTTGTCCCC CAGCCTATAC CATGGTCGGA CTGCATAACC TGGAGGCCCG CGTGGATAGG 
AACACTGCCT TTCAACCAGT GAGCTTCCAG GAAGAGAAGG CTGGGAGGGA AGAGCTGCCC 
TCAGCTCACG AGAGCTTTCG CCAGAAGCTG GCTGCCTTTG CAGGGATGAC ATCCAGCTGC 
CCCAAGGGCC CCAGGCCCTG CACTTCTCCA CAGCCCCTGC GGGAATCCCT GCCCTCGGAG 
GATGACAGTG ACCAAAGGTG CTCACCCTCA GGAGACAGCG AAGGTGGGGA GTACTGTTCC 
ATTCTGGACT GTTGTCCAGA ATCCAAAGAT GCTGTGCATA GCACCGAGGG CTCTGGCAGA 
CGTGGAGGGG ACTGCTCACC CACGTGCAGG GAGCAGGGAC CACGTACAAG ACCAACAGAA 
GAGGAAAAAC AGGGTCTGAG CTTCCCCAGA GAGTGCTGTG GTCAGGGATC CACAGCGAAC 
CCGCCCCGCC TGGGCCCCAA GAAGCCATCC CTTAACTCTG AGGCCGCCAG CTCTTCCGAT 
GGTCTGTCCT GTGGCAGCAG CCGCAGCGGG GCCAGCAGCC CCTTCGCTCC CCACCTTGAG 
AACGACTACT GCTCTCTTGT GAAGGAACCG GCCTCAGGGA AGCAGCAAGA CCTCAGCGGC 
CACTTTCTCA CCTCTGGCAA ATGTGTGGGT CAAGCTGCGG AACTCCAGCC CGCCTCCCTT 
CTTAGGGACC CTGTGCAGCC TGAACCCATC TACGCCGAAA GCGCCAAGAG GAAGAAGGCA 
GCTCCGGGGC CTCCGAGGCC CGAGCCCAAG AAAGAGCAGG TCCCAGCTGG CCATAGCCAG 
GGCCAAGTAT GGACAGGTGA TACCTGGATT CAGAAGACAC CCCCTAGCTG GAGCCAGGAC 
AGGGAAGGCG CAAACCCTGC TCCTCAGGTG GCAACCACCA TCACTGTCAT CGCTGCTCAC 
CCGGAAGAGG ACCACAGGAC TATTTATCTG AGCAGCCCTG ACTCTGCCGT GGGGGTGCAG 
TGGCCACGTG GGCCTTCGAA CCAGGACTTA CAAGCCGGTG AAGAGGAGCC TTTGGTTGCG 
CAGGGGCTAA CCTCAAGGGA GAGCCATCCA CATAATGTGA CTGAAAACAC GGCCAAAGAG 
AAGCCTGCCA TTCCCCCCAA ACTGTCCAAA AGCAGCCCCG GAGGGTCCCC TGTGTCTCCC 
GCGCCCCCAC TGACTGACCA CAGCGACGGG AACACCGGTG GCAGCAGCGT CGGACCCCAA 
CTTTTGTCTA GAGTCCCTGC TAACCTAACG TCTTCCTGCC ACACCAATGG CGTTGCCACT 
GCTGGTGACT CTGCCAAGTG TCCCCCACCA GCCACCTCCT CTTCAGTCTT GGACCAGAGG 
CGGCCAAGAT ACCAGACTGG TGCTTGGAGC CGCCAGTGCC GGATAGAAGA GGAGGAGGAG 
GTGGGGCAGG AGTTGAGTCA AAGTTGGGGG AGAGAGTTGG AAAACGGTAC CGCAGACCAT 
TCAAACTCCT CTACCTGGCA CCGTCTCCAT CCCATCGATG GCACCTCTGG GCAGAACAGC 
AAAACTAACT CTGGGATGAG CAAATCGGCT TCCTTTGCGT TTGAGTTCCC AAAGGACAGA 
GGCAGATTGG AGGCATTCTC ACCCCCTCCC CCACCTCCAA AGTCAAGGCA CCTGTTAAAA 
ATGAACAAGA GCAGTTCTGA CTTGGAAAAA GTGAGCCAGA GTTCTGCAGA AAGCCTCAGC 
CCGTCCTTCA GAGGGGCCCA CGTCAGCTTC ACCACGGGCT CCACAGATAG CCTAGCCTCG 
GACTCCAGGC CCTGCAGCGA TGGAGGTCCA TCATATGAAC CCACCCACTC ACCCACCATC 
AGCGGGAAAA AGCTCTTCGC TCCCGTCCCT TTTCCATCAG GCTCCACAGA GGATGTGTCC 
CCTGGTGGTG GTCCTGCGCA GCCTCCTCCT CTGCCCCAGA AAAAGATAGT GAGCCGTGCC 
GCCTCTTCCC CTGATGGCTT CTTCTGGACC CAAGGTTCCC CTAAGCCACG GACGGCAAGT 
CCCAAGCTGA ACCTAAGCCA TTCAGAAACC AATGTTTGTG CCCATGACGA GCCTCCTTTT 
AACTGTTCCT TGAACTCTGG AAACCGTTCC CACCATGTCT TCTCCTCTTC TGAGCCTTTG 
GGGAAAGCTT TCAAAGGCAA TGCTCCCTGG GCCCCAGCTC TGGGGCTGGC GAACAGCAAA 
GGCGGCTGTG GGAGCCCCAG TCTCCAGTGC AGAGCGGCCA CCTCCACGTC ATCATCCCAG 
CTCAGCGTGT CCAGCCAGGC CTCCTCCAGC AGCACCCAGC TTCAGCTGCA CAGCCTGCTG 
AGTAGCATCA GCAGCAAGGA GGGCACCTAT GCCAAGCTTG GGGGCCTCTA CACACAATCC 
CTGGCCCGCC TAGTGACCAA GTGTGAAGAT CTCTTCATGG GTGGCCAGAA AAAGGAACTG 
CGCTTCAATG AGAATTACTG GTCACTCTTC AAGCTGACGT GCAACAAACC TTGCTGTGAC 
TCGGGGGATG CAATTTACTA CTGTGCCACC TGCTCTGAGG ACCCAGGCAG CATCTATGCT 
GTGAAAATCT GCAAAACCCC AGAACCCAAA TCAGCCTCAT ACTGCAGCCC ATCTGTGCCG 
GTGCATTTCA ACATCCAGCA AGACTGCGGC CACTTTGTGG CCTCTGTGCC CTCCAGCATG 
CTTGCCTCCC CTGACACATC CAGCAAAGAC ACCGCGCCTG CCGTATCCCC ACAGCCCCCT 
GCCCAGGAGC AGGACTGTGT GGTGGTCATC ACCCGGGAGG TACCGCACCA GACTGCCTCC 
GACTTTGTGC GGGACTCTAT GGCCAGCCAC CGGGCGGAGC CTGAGGTTTA CGAGCGCCGG 
GTGTGCTTCC TGCTTCTGCA GCTCTGCAAC GGGCTGGAGC ACCTGAAGGA ACACGGGATC 
ATCCACCGGG ACCTGTGTCT GGAGAACCTG CTGCTGGCGC ACTGTAACCC TCAGAGCTCC 
CCCGGGCCCT CTGCCACTCC TACCGTGCCC ACCACCACAT CCAGGTGCCC TTCTGCCGCG 
CCCGCAGCCA CCACTGCCTG CCAGGGAGGG CCTGGTGAGA AGCAGCTGCC CAGGCTCATC 
ATCAGCAACT TCCTGAAGGC CAAGCAAAAG CCAGGAGGCA CCACCAACCT GCAACAGAAG 
AAGAGCCAGG CCCGGCTGGC TCCCGAGATC GTGTCTGCCT CCCAGTACCG CAAGTTTGAT 
GAGTTCCAGA CGGGCATCCT CATCTATGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGAGCCCAGC TCCGGGAGCG CGACTACCGG CGGGAGGACT TACCCCCGCT GCCCACACTG 
TCCCTCTACT CTCCCGGCCT GCAGCAACTC GCCCATCTAC TGCTTGAGGC TGATCCCATC 
AAGCGCATCC GCATAGGTGA AGCTAAGCGG GTGCTGCAGT GCTTGCTGTG GGGGCCACGG 
CGGGAGCTGG TGGAGCAGCC GTGCACGTCA GAGGAGGTAC TGTGTAACAC TCTGCACAAC 
TGGATCGACA TGAAGCGAGC ACTGATGATG ATGAAGTTTG CTGAGAAGGC GGTGGACCGA 
AGGCGGGGTG TGGAACTGGA GGACTGGCTT TGCTGCCAGT ACTTGGCCTC GGCAGAGCCA 
GGGGCCCTCT TGCAGTCCCT GAAGCTCCTA CAACTTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NACK is an integral component of the Notch transcriptional activation complex and is critical for development and tumorigenesis.
Cancer research74(17)4741-51(2014 Sep)
Weaver KL,Alves-Guerra MC,Jin K,Wang Z,Han X,Ranganathan P,Zhu X,DaSilva T,Liu W,Ratti F,Demarest RM,Tzimas C,Rice M,Vasquez-Del Carpio R,Dahmane N,Robbins DJ,Capobianco AJ

Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity.
The Journal of biological chemistry281(15)10355-64(2006 Apr)
Tanaka H,Katoh H,Negishi M

The mouse kinome: discovery and comparative genomics of all mouse protein kinases.
Proceedings of the National Academy of Sciences of the United States of America101(32)11707-12(2004 Aug)
Caenepeel S,Charydczak G,Sudarsanam S,Hunter T,Manning G

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Prag1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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