PRAG1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011543813.2	XP_011542115.1	tyrosine-protein kinase SgK223 isoform X2
NM_001080826.2	NP_001074295.2	inactive tyrosine-protein kinase PRAG1
XM_005272370.4	XP_005272427.2	tyrosine-protein kinase SgK223 isoform X1
XM_005272370.5	XP_005272427.2	tyrosine-protein kinase PRAG1 isoform X1
NM_001080826.3	NP_001074295.2	inactive tyrosine-protein kinase PRAG1
NM_001369759.1	NP_001356688.1	inactive tyrosine-protein kinase PRAG1
XM_011543813.3	XP_011542115.1	tyrosine-protein kinase PRAG1 isoform X2
XM_005272369.4	XP_005272426.2	tyrosine-protein kinase SgK223 isoform X1
XM_005272369.5	XP_005272426.2	tyrosine-protein kinase PRAG1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu76878	XM_011543813.3 Latest version!	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu15038	NM_001080826.3 Latest version!	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu15038	NM_001369759.1 Latest version!	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu15038	XM_005272369.5 Latest version!	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu15038	XM_005272370.5 Latest version!	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OHu15038	XM_005272370.4	Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu15038	NM_001080826.2	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu76878	XM_011543813.2	Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu15038	XM_005272369.4	Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

PRAG1 ( XM_011543813.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542115.1 Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu76878
Clone ID Related Accession (Same CDS sequence)	XM_011543813.2 , XM_011543813.3
Accession Version	XM_011543813.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase SgK223 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGAACCATAT TGTGTTTATT CATTCGTCTG TTAATGGATA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542115.1
CDS	212..2416
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRTILCLFIRLLMDT

Target ORF information:

RefSeq Version	XM_011543813.2
Organism	Homo sapiens(human)
Definition	Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543813.2

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGAACCATAT TGTGTTTATT CATTCGTCTG TTAATGGATA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_011543813.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542115.1 Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu76878
Clone ID Related Accession (Same CDS sequence)	XM_011543813.2 , XM_011543813.3
Accession Version	XM_011543813.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase PRAG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGAACCATAT TGTGTTTATT CATTCGTCTG TTAATGGATA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542115.1
CDS	209..2413
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRTILCLFIRLLMDT

Target ORF information:

RefSeq Version	XM_011543813.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543813.3

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGAACCATAT TGTGTTTATT CATTCGTCTG TTAATGGATA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( NM_001080826.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074295.2 Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), mRNA.

Price & Availability↑

CloneID	OHu15038
Clone ID Related Accession (Same CDS sequence)	NM_001080826.2 , XM_005272370.4 , XM_005272370.5 , NM_001080826.3 , NM_001369759.1 , XM_005272369.4 , XM_005272369.5
Accession Version	NM_001080826.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	inactive tyrosine-protein kinase PRAG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX420505.2, AK122582.1, AC103957.8, AC068353.34, ABBA01026926.1 and AL833872.1. On Mar 19, 2014 this sequence version replaced NM_001080826.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to add two internal 6-nt sequences that were included in the update of the GRCh37 reference genome assembly to the GRCh38 assembly. The update results in a protein that is 4 aa longer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074295.2
CDS	88..4308
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	NM_001080826.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080826.2

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( NM_001080826.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074295.2 Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant 1, mRNA.

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CloneID	OHu15038
Clone ID Related Accession (Same CDS sequence)	NM_001080826.2 , XM_005272370.4 , XM_005272370.5 , NM_001080826.3 , NM_001369759.1 , XM_005272369.4 , XM_005272369.5
Accession Version	NM_001080826.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	inactive tyrosine-protein kinase PRAG1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC103957.8, BX420505.2, AK122582.1, AC068353.34, ABBA01026926.1 and AL833872.1. On or before Apr 17, 2019 this sequence version replaced XM_005272369.5, NM_001080826.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to add two internal 6-nt sequences that were included in the update of the GRCh37 reference genome assembly to the GRCh38 assembly. The update results in a protein that is 4 aa longer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2109290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000615670.5/ ENSP00000481109.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074295.2
CDS	207..4427
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	NM_001080826.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080826.3

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( NM_001369759.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356688.1 Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant 2, mRNA.

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CloneID	OHu15038
Clone ID Related Accession (Same CDS sequence)	NM_001080826.2 , XM_005272370.4 , XM_005272370.5 , NM_001080826.3 , NM_001369759.1 , XM_005272369.4 , XM_005272369.5
Accession Version	NM_001369759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	inactive tyrosine-protein kinase PRAG1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC103957.8, BX420505.2, AK122582.1, AC068353.34, ABBA01026926.1 and AL833872.1. On Apr 17, 2019 this sequence version replaced XM_005272370.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK122582.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END##

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356688.1
CDS	678..4898
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	NM_001369759.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369759.1

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_005272370.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272427.2 Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X2, mRNA.

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CloneID	OHu15038
Clone ID Related Accession (Same CDS sequence)	NM_001080826.2 , XM_005272370.4 , XM_005272370.5 , NM_001080826.3 , NM_001369759.1 , XM_005272369.4 , XM_005272369.5
Accession Version	XM_005272370.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272370.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272427.2
CDS	676..4896
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005272370.4
Organism	Homo sapiens(human)
Definition	Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272370.4

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_005272370.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272427.2 Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu15038
Clone ID Related Accession (Same CDS sequence)	NM_001080826.2 , XM_005272370.4 , XM_005272370.5 , NM_001080826.3 , NM_001369759.1 , XM_005272369.4 , XM_005272369.5
Accession Version	XM_005272370.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase PRAG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005272370.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272427.2
CDS	678..4898
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005272370.5
Organism	Homo sapiens(human)
Definition	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272370.5

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_005272369.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272426.2 Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X1, mRNA.

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CloneID	OHu15038
Clone ID Related Accession (Same CDS sequence)	NM_001080826.2 , XM_005272370.4 , XM_005272370.5 , NM_001080826.3 , NM_001369759.1 , XM_005272369.4 , XM_005272369.5
Accession Version	XM_005272369.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272369.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272426.2
CDS	211..4431
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005272369.4
Organism	Homo sapiens(human)
Definition	Homo sapiens homolog of rat pragma of Rnd2 (SGK223), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272369.4

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_005272369.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272426.2 Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X1, mRNA.

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CloneID	OHu15038
Clone ID Related Accession (Same CDS sequence)	NM_001080826.2 , XM_005272370.4 , XM_005272370.5 , NM_001080826.3 , NM_001369759.1 , XM_005272369.4 , XM_005272369.5
Accession Version	XM_005272369.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase PRAG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005272369.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272426.2
CDS	209..4429
Translation	MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005272369.5
Organism	Homo sapiens(human)
Definition	Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272369.5

ORF Insert Sequence:

ATGCACCAGA CCCTCTGCCT GAACCCCGAG AGCCTGAAAA TGTCTGCGTG CAGTGACTTT 
GTGGAGCACA TCTGGAAACC CGGGTCCTGC AAGAACTGCT TCTGCCTGCG GAGCGACCAC 
CAGCTGGTGG CCGGCCCTCC CCAGCCCAGA GCGGGCAGCC TGCCCCCTCC ACCGCGCCTG 
CCTCCCAGGC CTGAGAACTG CCGCCTGGAA GATGAAGGTG TGAACAGCTC ACCTTACTCC 
AAGCCCACAA TTGCCGTGAA GCCCACCATG ATGAGCTCCG AGGCCTCTGA TGTGTGGACA 
GAGGCCAACC TGAGTGCCGA AGTCTCGCAG GTCATCTGGA GACGAGCCCC TGGCAAGCTC 
CCCCTCCCGA AGCAGGAGGA TGCCCCCGTC GTCTACCTGG GCAGCTTCCG AGGTGTACAG 
AAGCCTGCTG GTCCCTCTAC CTCCCCTGAT GGCAATTCTC GCTGTCCCCC AGCTTACACC 
ATGGTCGGCC TGCACAACCT TGAGCCCCGC GGCGAGAGGA ACATTGCCTT CCACCCGGTG 
AGCTTCCCGG AGGAGAAGGC TGTGCACAAA GAAAAACCCT CATTTCCTTA CCAAGACCGG 
CCCTCCACCC AGGAGAGCTT CCGCCAGAAA CTGGCTGCCT TTGCTGGGAC CACATCTGGC 
TGTCACCAGG GCCCTGGGCC CCTGCGGGAA TCCCTGCCCT CGGAGGATGA CAGTGATCAA 
AGGTGCTCGC CCTCCGGGGA CAGCGAGGGT GGAGAGTACT GCTCCATCCT GGACTGCTGC 
CCTGGGAGCC CTGTTGCCAA GGCTGCCTCC CAGACTGCAG GTTCCCGGGG CAGGCATGGT 
GGCAGGGACT GCTCACCCAC GTGCTGGGAG CAGGGGAAGT GTTCCGGGCC CGCAGAGCAG 
GAGAAGCGGG GCCCGAGCTT CCCCAAGGAG TGCTGTAGCC AGGGCCCCAC TGCCCACCCA 
TCCTGCCTGG GCCCCAAGAA ACTGTCCCTC ACCTCGGAGG CTGCCATTTC TTCCGACGGC 
CTCTCTTGTG GCAGCGGCAG CGGCAGCGGC AGCGGCGCCA GTAGCCCCTT CGTCCCCCAC 
CTCGAGAGTG ATTACTGCTC CCTCATGAAG GAACCTGCCC CAGAGAAGCA GCAGGACCCT 
GGCTGCCCAG GGGTGACCCC TAGCAGATGC CTTGGGCTGA CGGGGGAGCC CCAGCCCCCG 
GCCCACCCCC GGGAGGCTAC ACAGCCTGAA CCCATCTATG CTGAGAGCAC CAAGAGGAAG 
AAGGCAGCTC CGGTGCCTTC CAAGTCACAG GCCAAGATAG AACATGCAGC TGCTGCCCAG 
GGCCAAGGCC AGGTATGCAC AGGTAATGCC TGGGCCCAGA AAGCAGCATC TGGCTGGGGC 
CGGGACAGCC CAGACCCAAC TCCCCAGGTG TCAGCCACCA TCACAGTCAT GGCGGCCCAC 
CCGGAAGAGG ACCATCGGAC GATCTACCTG AGCAGCCCTG ACTCTGCAGT GGGGGTGCAG 
TGGCCACGAG GGCCTGTGAG CCAGAACTCC GAGGTAGGTG AAGAGGAGAC TTCGGCTGGG 
CAGGGGCTGA GCTCCAGGGA AAGCCATGCT CACAGTGCCA GCGAGAGCAA GCCCAAGGAG 
AGGCCCGCCA TTCCCCCCAA GTTGTCCAAG AGTAGCCCTG TAGGGTCCCC GGTGTCACCG 
TCTGCTGGAG GGCCCCCAGT GTCACCGCTG GCTGACCTTA GTGATGGGAG CTCTGGCGGC 
AGCAGCATTG GGCCCCAGCC TCCATCCCAA GGTCCTGCTG ACCCCGCTCC TTCCTGCCGG 
ACCAACGGTG TCGCTATCAG TGACCCATCC AGGTGTCCCC AGCCTGCCGC CTCGTCAGCC 
TCGGAACAGA GGCGGCCCAG GTTCCAGGCA GGCACCTGGA GTCGTCAGTG CCGGATAGAG 
GAAGAAGAGG AGGTGGAGCA GGAATTGCTG AGTCACAGCT GGGGAAGAGA GACCAAAAAT 
GGCCCCACGG ACCATTCAAA CTCCACGACC TGGCACCGTC TCCACCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGT TGGGACCGGG ATGAGCAAAT CCGCCTCTTT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGTGG GATTGAGACA TTCTCACCTC CTCCTCCGCC TCCAAAGTCG 
CGGCACCTTC TAAAAATGAA CAAGAGCAGC TCTGATTTGG AAAAAGTGAG CCAGGGCTCT 
GCAGAAAGCC TCAGCCCATC CTTCAGGGGT GTCCACGTCA GCTTCACCAC CGGCTCCACG 
GACAGCCTGG CCTCAGACTC TAGGACCTGC AGCGATGGAG GTCCCTCGTC TGAGCTGGCT 
CACTCGCCCA CCAACAGCGG GAAGAAGCTC TTTGCTCCCG TTCCGTTTCC TTCAGGCTCC 
ACTGAGGACG TGTCCCCCAG TGGCCCCCAG CAGCCCCCTC CACTCCCCCA GAAAAAGATA 
GTGAGCCGGG CAGCCTCTTC ACCGGATGGC TTCTTCTGGA CCCAAGGCTC CCCCAAGCCC 
GGAACAGCAA GCCCCAAGCT GAACCTAAGC CACTCGGAAA CCAACGTCCA CGACGAATCT 
CACTTTAGCT ATTCGTTGAG CCCCGGGAAC CGCCACCATC CTGTCTTCTC CTCTTCCGAT 
CCTCTGGAGA AAGCTTTCAA AGGCAGTGGC CACTGGCTTC CGGCAGCAGG GCTGGCGGGC 
AACAGAGGCG GCTGCGGGAG CCCTGGCCTC CAGTGCAAAG GGGCCCCCTC CGCCTCATCC 
TCCCAGCTGA GCGTGTCCAG TCAAGCCTCC ACCGGGAGCA CCCAGCTTCA GCTGCACGGT 
CTCCTGAGCA ACATCAGCAG CAAGGAGGGC ACCTATGCCA AGCTGGGGGG ACTCTACACC 
CAGTCCCTGG CCCGCCTTGT AGCCAAATGT GAGGACCTCT TCATGGGCGG CCAGAAAAAG 
GAGCTCCACT TCAATGAGAA TAACTGGTCG CTCTTCAAGC TGACTTGTAA CAAGCCCTGC 
TGTGACTCGG GGGATGCCAT TTATTACTGT GCCACCTGCT CTGAGGACCC CGGCAGCACC 
TATGCTGTGA AAATCTGCAA AGCCCCTGAG CCCAAAACAG TCTCCTACTG CAGCCCGTCC 
GTGCCCGTGC ACTTTAACAT CCAGCAGGAC TGCGGCCACT TCGTCGCCTC GGTGCCGTCC 
AGCATGCTCA GCTCCCCCGA CGCGCCCAAG GACCCTGTGC CTGCCCTGCC CACACACCCC 
CCTGCCCAGG AGCAGGACTG CGTGGTGGTC ATCACCCGAG AGGTGCCACA TCAGACCGCC 
TCCGACTTCG TGCGGGACTC GGCGGCCAGC CACCAGGCGG AGCCCGAGGC GTACGAGCGG 
CGCGTGTGCT TCCTGCTTCT GCAACTCTGC AACGGGCTGG AGCACCTGAA GGAGCACGGG 
ATCATCCACC GGGACCTGTG CCTGGAGAAC CTGCTGCTGG TGCACTGCAC CCTCCAGGCC 
GGCCCCGGGC CCGCCCCCGC CCCCGCCCCG GCTCCCGCCC CCGCCGCCGC CGCGCCTCCC 
TGCTCCTCTG CCGCCCCGCC TGCTGGTGGC ACTCTCAGCC CCGCAGCCGG CCCCGCCTCC 
CCGGAAGGGC CCCGGGAGAA GCAGCTGCCC CGGCTCATCA TCAGCAACTT TTTGAAGGCC 
AAGCAGAAGC CGGGCGGCAC CCCAAACCTG CAGCAGAAGA AGAGCCAGGC CCGGCTGGCC 
CCCGAGATCG TGTCTGCTTC CCAGTACCGC AAGTTCGATG AGTTCCAGAC AGGCATCCTC 
ATCTACGAGC TGCTGCACCA ACCCAACCCG TTCGAGGTGC GCGCCCAGCT GCGGGAGAGA 
GACTACCGGC AGGAGGACCT GCCGCCGCTG CCCGCGCTGT CCCTCTACTC ACCCGGCCTG 
CAGCAGCTGG CACATCTGCT ACTGGAGGCC GACCCCATCA AGCGTATCCG CATCGGCGAG 
GCCAAGCGCG TGCTGCAGTG CCTGCTGTGG GGGCCTCGGC GCGAGCTGGT GCAGCAGCCG 
GGCACCTCGG AGGAGGCGCT GTGCGGCACG CTGCACAACT GGATCGACAT GAAGCGGGCC 
CTGATGATGA TGAAGTTTGC GGAGAAGGCG GTGGATCGCA GGCGGGGCGT GGAGCTGGAG 
GACTGGCTTT GCTGCCAGTA CCTGGCGTCT GCGGAGCCCG GGGCCCTCTT ACAGTCGCTG 
AAGCTCCTGC AGCTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PRAG1
Entrez Gene ID	157285
Full Name	PEAK1 related, kinase-activating pseudokinase 1
Synonyms	NACK,PEAK2,PRAGMIN,SGK223
General protein information	Preferred Names PEAK1 related, kinase-activating pseudokinase 1 Names tyrosine-protein kinase PRAG1 tyrosine-protein kinase SgK223 PEAK family member 2 Pragmin, RND2 effector protein homolog of rat pragma of Rnd2 sugen kinase 223
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p23.1
Summary	This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014].
Disorder MIM:	617344

Bos taurus (cattle)	LOC533821	XP_005226093.1
Canis lupus familiaris (dog)	LOC482885	XP_540000.3
Xenopus tropicalis (tropical clawed frog)	LOC100486686	XP_002936500.1
Mus musculus (house mouse)	D8Ertd82e	NP_766499.2
Rattus norvegicus (Norway rat)	Pragmin	NP_001100785.1
Homo sapiens (human)	SGK223	NP_001074295.1
Macaca mulatta (Rhesus monkey)	LOC706349	XP_001094718.1
Pan troglodytes (chimpanzee)	LOC472692	XP_001136236.1
Gallus gallus (chicken)	SGK223	XP_420588.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PRAG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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