Rest cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rest
Entrez Gene ID	19712
Full Name	RE1-silencing transcription factor
Synonyms	2610008J04Rik,AA407358,NRSF,REST4
General protein information	Preferred Names RE1-silencing transcription factor Names RE1-silencing transcription factor neural-restrictive silencer factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534842.2	XP_006534905.1	RE1-silencing transcription factor isoform X1
XM_006534842.3	XP_006534905.1	RE1-silencing transcription factor isoform X1
NM_011263.2	NP_035393.2	RE1-silencing transcription factor
XM_006534842.4	XP_006534905.1	RE1-silencing transcription factor isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rest	NP_113976.1
Bos taurus (cattle)	REST	XP_005208055.1
Gallus gallus (chicken)	REST	XP_420580.4
Pan troglodytes (chimpanzee)	REST	XP_003310418.1
Canis lupus familiaris (dog)	REST	XP_539282.3
Homo sapiens (human)	REST	NP_001180437.1
Mus musculus (house mouse)	Rest	NP_035393.2
Macaca mulatta (Rhesus monkey)	REST	XP_002804152.1

Gene Ontology
Bibliography

Related articles in PubMed

Overlapping Activities of Two Neuronal Splicing Factors Switch the GABA Effect from Excitatory to Inhibitory by Regulating REST.
Nakano Y, Wiechert S, Bánfi B
Cell reports27(3)860-871.e8(2019 04)

RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and
Zhang J, Chen SR, Chen H, Pan HL
The Journal of biological chemistry293(49)19078-19091(2018 12)

Sertraline, chlorprothixene, and chlorpromazine characteristically interact with the REST-binding site of the corepressor mSin3, showing medulloblastoma cell growth inhibitory activities.
Kurita JI, Hirao Y, Nakano H, Fukunishi Y, Nishimura Y
Scientific reports8(1)13763(2018 09)

Defects in the Alternative Splicing-Dependent Regulation of REST Cause Deafness.
Nakano Y, Kelly MC, Rehman AU, Boger ET, Morell RJ, Kelley MW, Friedman TB, Bánfi B
Cell174(3)536-548.e21(2018 07)

The Epigenetic Factor Landscape of Developing Neocortex Is Regulated by Transcription Factors Pax6→ Tbr2→ Tbr1.
Elsen GE, Bedogni F, Hodge RD, Bammler TK, MacDonald JW, Lindtner S, Rubenstein JLR, Hevner RF
Frontiers in neuroscience12571(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rest gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rest cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20217	NM_011263.2 Latest version!	Mus musculus RE1-silencing transcription factor (Rest), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$559.30 $799.00
OMu20217	XM_006534842.4 Latest version!	Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$559.30 $799.00
View Old Accession Versions >>
OMu20217	XM_006534842.2	Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OMu20217	XM_006534842.3	Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rest ( XM_006534842.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534905.1 Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20217
Clone ID Related Accession (Same CDS sequence)	XM_006534842.2 , XM_006534842.3 , NM_011263.2 , XM_006534842.4
Accession Version	XM_006534842.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423324800000
Organism	Mus musculus(House mouse)
Product	RE1-silencing transcription factor isoform X1
Comment

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006534905.1

Translation

MATQVMGQSSGGGSLFNNSANMGMALTNDMYDLHELSKAELAAPQLIMLANVALTGEASGSCCDYLVGEERQMAELMPVGDNHFSESEGEGLEESADLKGLENMELGSLELSAVEPQPVFEASAAPEIYSANKDPAPETPVAEDKCRSSKAKPFRCKPCQYEAESEEQFVHHIRIHSAKKFFVEESAEKQAKAWESGSSPAEEGEFSKGPIRCDRCGYNTNRYDHYMAHLKHHLRAGENERIYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKKREADLLNNAVSNEKMENEQTKTKGDVSGKKNEKPVKAVGKDASKEKKPGSSVSVVQVTTRTRKSAVAAETKAAEVKHTDGQTGNNPEKPCKAKKNKRKKDAEAHPSEEPVNEGPVTKKKKKSECKSKIGTNVPKGGGRAEERPGVKKQSASLKKGTKKTPPKTKTSKKGGKLAPKGMGQTEPSSGALAQVGVSPDPALIQAEVTGSGSSQTELPSPMDIAKSEPAQMEVSLTGPPPVEPAQMEPSPAKPPQVEAPTYPQPPQRGPAPPTGPAPPTGPAPPTEPAPPTGLAEMEPSPTEPSQKEPPPSMEPPCPEELPQAEPPPMEDCQKELPSPVEPAQIEVAQTAPTQVQEEPPPVSEPPRVKPTKRSSLRKDRAEKELSLLSEMARQEQVLMGVGLVPVRDSKLLKGNKSAQDPPAPPSPSPKGNSREETPKDQEMVSDGEGTIVFPLKKGGPEEAGESPAELAALKESARVSSSEQNSAMPEGGASHSKCQTGSSGLCDVDTEQKTDTVPMKDSAAEPVSPPTPTVDRDAGSPAVVASPPITLAENESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPTPPEATSKNGKAGLAGKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQEEQEEREEQE

Target ORF information:

RefSeq Version	XM_006534842.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534842.2

ORF Insert Sequence:

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rest ( NM_011263.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035393.2 Mus musculus RE1-silencing transcription factor (Rest), mRNA.

Price & Availability↑

CloneID	OMu20217
Clone ID Related Accession (Same CDS sequence)	XM_006534842.2 , XM_006534842.3 , NM_011263.2 , XM_006534842.4
Accession Version	NM_011263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Mus musculus(house mouse)
Product	RE1-silencing transcription factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK004945.2, BC127065.1, BE132774.1 and AI121875.1. On Jul 24, 2009 this sequence version replaced NM_011263.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK004945.2, AK156514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035393.2
CDS	305..3553
Translation	MATQVMGQSSGGGSLFNNSANMGMALTNDMYDLHELSKAELAAPQLIMLANVALTGEASGSCCDYLVGEERQMAELMPVGDNHFSESEGEGLEESADLKGLENMELGSLELSAVEPQPVFEASAAPEIYSANKDPAPETPVAEDKCRSSKAKPFRCKPCQYEAESEEQFVHHIRIHSAKKFFVEESAEKQAKAWESGSSPAEEGEFSKGPIRCDRCGYNTNRYDHYMAHLKHHLRAGENERIYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKKREADLLNNAVSNEKMENEQTKTKGDVSGKKNEKPVKAVGKDASKEKKPGSSVSVVQVTTRTRKSAVAAETKAAEVKHTDGQTGNNPEKPCKAKKNKRKKDAEAHPSEEPVNEGPVTKKKKKSECKSKIGTNVPKGGGRAEERPGVKKQSASLKKGTKKTPPKTKTSKKGGKLAPKGMGQTEPSSGALAQVGVSPDPALIQAEVTGSGSSQTELPSPMDIAKSEPAQMEVSLTGPPPVEPAQMEPSPAKPPQVEAPTYPQPPQRGPAPPTGPAPPTGPAPPTEPAPPTGLAEMEPSPTEPSQKEPPPSMEPPCPEELPQAEPPPMEDCQKELPSPVEPAQIEVAQTAPTQVQEEPPPVSEPPRVKPTKRSSLRKDRAEKELSLLSEMARQEQVLMGVGLVPVRDSKLLKGNKSAQDPPAPPSPSPKGNSREETPKDQEMVSDGEGTIVFPLKKGGPEEAGESPAELAALKESARVSSSEQNSAMPEGGASHSKCQTGSSGLCDVDTEQKTDTVPMKDSAAEPVSPPTPTVDRDAGSPAVVASPPITLAENESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPTPPEATSKNGKAGLAGKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQEEQEEREEQE

Target ORF information:

RefSeq Version	NM_011263.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RE1-silencing transcription factor (Rest), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011263.2

ORF Insert Sequence:

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rest ( XM_006534842.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534905.1 Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

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CloneID	OMu20217
Clone ID Related Accession (Same CDS sequence)	XM_006534842.2 , XM_006534842.3 , NM_011263.2 , XM_006534842.4
Accession Version	XM_006534842.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	RE1-silencing transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534905.1
CDS	551..3799
Translation	MATQVMGQSSGGGSLFNNSANMGMALTNDMYDLHELSKAELAAPQLIMLANVALTGEASGSCCDYLVGEERQMAELMPVGDNHFSESEGEGLEESADLKGLENMELGSLELSAVEPQPVFEASAAPEIYSANKDPAPETPVAEDKCRSSKAKPFRCKPCQYEAESEEQFVHHIRIHSAKKFFVEESAEKQAKAWESGSSPAEEGEFSKGPIRCDRCGYNTNRYDHYMAHLKHHLRAGENERIYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKKREADLLNNAVSNEKMENEQTKTKGDVSGKKNEKPVKAVGKDASKEKKPGSSVSVVQVTTRTRKSAVAAETKAAEVKHTDGQTGNNPEKPCKAKKNKRKKDAEAHPSEEPVNEGPVTKKKKKSECKSKIGTNVPKGGGRAEERPGVKKQSASLKKGTKKTPPKTKTSKKGGKLAPKGMGQTEPSSGALAQVGVSPDPALIQAEVTGSGSSQTELPSPMDIAKSEPAQMEVSLTGPPPVEPAQMEPSPAKPPQVEAPTYPQPPQRGPAPPTGPAPPTGPAPPTEPAPPTGLAEMEPSPTEPSQKEPPPSMEPPCPEELPQAEPPPMEDCQKELPSPVEPAQIEVAQTAPTQVQEEPPPVSEPPRVKPTKRSSLRKDRAEKELSLLSEMARQEQVLMGVGLVPVRDSKLLKGNKSAQDPPAPPSPSPKGNSREETPKDQEMVSDGEGTIVFPLKKGGPEEAGESPAELAALKESARVSSSEQNSAMPEGGASHSKCQTGSSGLCDVDTEQKTDTVPMKDSAAEPVSPPTPTVDRDAGSPAVVASPPITLAENESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPTPPEATSKNGKAGLAGKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQEEQEEREEQE

Target ORF information:

RefSeq Version	XM_006534842.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534842.3

ORF Insert Sequence:

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rest ( XM_006534842.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534905.1 Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20217
Clone ID Related Accession (Same CDS sequence)	XM_006534842.2 , XM_006534842.3 , NM_011263.2 , XM_006534842.4
Accession Version	XM_006534842.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	RE1-silencing transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534842.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534905.1
CDS	401..3649
Translation	MATQVMGQSSGGGSLFNNSANMGMALTNDMYDLHELSKAELAAPQLIMLANVALTGEASGSCCDYLVGEERQMAELMPVGDNHFSESEGEGLEESADLKGLENMELGSLELSAVEPQPVFEASAAPEIYSANKDPAPETPVAEDKCRSSKAKPFRCKPCQYEAESEEQFVHHIRIHSAKKFFVEESAEKQAKAWESGSSPAEEGEFSKGPIRCDRCGYNTNRYDHYMAHLKHHLRAGENERIYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKKREADLLNNAVSNEKMENEQTKTKGDVSGKKNEKPVKAVGKDASKEKKPGSSVSVVQVTTRTRKSAVAAETKAAEVKHTDGQTGNNPEKPCKAKKNKRKKDAEAHPSEEPVNEGPVTKKKKKSECKSKIGTNVPKGGGRAEERPGVKKQSASLKKGTKKTPPKTKTSKKGGKLAPKGMGQTEPSSGALAQVGVSPDPALIQAEVTGSGSSQTELPSPMDIAKSEPAQMEVSLTGPPPVEPAQMEPSPAKPPQVEAPTYPQPPQRGPAPPTGPAPPTGPAPPTEPAPPTGLAEMEPSPTEPSQKEPPPSMEPPCPEELPQAEPPPMEDCQKELPSPVEPAQIEVAQTAPTQVQEEPPPVSEPPRVKPTKRSSLRKDRAEKELSLLSEMARQEQVLMGVGLVPVRDSKLLKGNKSAQDPPAPPSPSPKGNSREETPKDQEMVSDGEGTIVFPLKKGGPEEAGESPAELAALKESARVSSSEQNSAMPEGGASHSKCQTGSSGLCDVDTEQKTDTVPMKDSAAEPVSPPTPTVDRDAGSPAVVASPPITLAENESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPTPPEATSKNGKAGLAGKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQEEQEEREEQE

Target ORF information:

RefSeq Version	XM_006534842.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534842.4

ORF Insert Sequence:

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGCA GTCTCTTCAA CAACAGTGCC 
AACATGGGCA TGGCCTTAAC CAACGACATG TACGACCTGC ACGAGCTCTC GAAAGCTGAA 
CTGGCAGCCC CTCAGCTCAT CATGTTAGCC AACGTGGCCC TGACGGGGGA GGCAAGCGGC 
AGCTGCTGCG ATTACCTGGT CGGTGAAGAG AGGCAGATGG CCGAATTGAT GCCCGTGGGA 
GACAACCACT TCTCAGAAAG TGAAGGAGAA GGCCTGGAAG AGTCGGCTGA CCTCAAAGGG 
CTGGAAAACA TGGAACTGGG AAGTTTGGAG CTAAGTGCTG TAGAACCCCA GCCCGTATTT 
GAAGCCTCAG CTGCCCCAGA AATATACAGC GCCAATAAAG ATCCCGCTCC AGAAACACCC 
GTGGCGGAAG ACAAATGCAG GAGTTCTAAG GCCAAGCCCT TCCGGTGTAA GCCTTGCCAG 
TACGAAGCCG AATCTGAAGA GCAGTTTGTG CATCACATCC GGATTCACAG CGCTAAGAAG 
TTCTTTGTGG AGGAAAGTGC AGAGAAACAG GCCAAAGCCT GGGAGTCGGG GTCGTCTCCG 
GCCGAAGAGG GCGAGTTCTC CAAAGGCCCC ATCCGCTGTG ACCGCTGTGG CTACAATACC 
AACCGGTATG ACCACTACAT GGCACACCTG AAGCACCACC TGCGAGCTGG CGAGAACGAG 
CGCATCTACA AGTGCATCAT CTGCACGTAC ACGACGGTCA GCGAGTACCA CTGGAGGAAA 
CACCTGAGAA ACCATTTCCC CAGGAAAGTC TACACCTGCA GCAAGTGCAA CTACTTCTCA 
GACAGAAAAA ATAACTACGT TCAGCACGTG CGAACTCACA CAGGAGAACG CCCGTATAAA 
TGTGAACTTT GTCCTTACTC AAGCTCTCAG AAGACTCATC TAACGCGACA CATGCGGACT 
CATTCAGGTG AGAAGCCATT TAAATGTGAT CAGTGCAATT ATGTGGCCTC TAATCAGCAT 
GAAGTGACCC GACATGCAAG ACAGGTTCAC AACGGGCCTA AACCTCTTAA TTGCCCGCAC 
TGTGACTACA AAACAGCAGA TAGAAGCAAC TTCAAAAAGC ACGTGGAGCT GCATGTTAAC 
CCACGGCAGT TCAACTGCCC CGTGTGTGAC TACGCGGCTT CTAAGAAGTG TAATCTACAA 
TACCATTTCA AATCTAAGCA TCCCACCTGT CCCAGCAAAA CAATGGATGT CTCCAAAGTG 
AAGCTAAAGA AAACCAAAAA GAGAGAGGCT GACCTGCTTA ATAACGCCGT CAGCAACGAG 
AAGATGGAGA ATGAGCAAAC AAAAACAAAG GGGGATGTGT CTGGGAAGAA GAACGAGAAA 
CCTGTAAAAG CTGTGGGAAA AGATGCTTCA AAAGAGAAGA AGCCTGGTAG CAGTGTCTCA 
GTGGTCCAGG TAACTACCAG GACTCGGAAG TCAGCGGTGG CGGCGGAGAC TAAAGCAGCA 
GAGGTGAAAC ACACAGACGG ACAAACAGGA AACAATCCAG AAAAGCCCTG TAAAGCCAAG 
AAAAACAAAA GAAAGAAGGA TGCTGAGGCC CATCCCTCCG AAGAGCCTGT GAACGAGGGA 
CCAGTGACAA AAAAGAAAAA GAAGTCTGAG TGCAAATCAA AAATCGGTAC CAACGTGCCA 
AAGGGCGGCG GCCGAGCGGA GGAGAGGCCG GGGGTCAAGA AGCAAAGCGC TTCCCTTAAG 
AAAGGCACAA AGAAGACGCC GCCCAAGACA AAGACAAGTA AAAAAGGTGG CAAACTTGCT 
CCAAAGGGGA TGGGGCAGAC AGAACCTTCT TCTGGGGCAT TGGCTCAAGT GGGGGTGTCT 
CCAGACCCTG CCCTCATTCA GGCAGAGGTC ACCGGGTCAG GATCTTCTCA GACAGAGCTT 
CCTTCACCCA TGGATATTGC TAAGTCAGAG CCCGCCCAGA TGGAGGTTTC CCTAACAGGG 
CCACCTCCGG TGGAGCCTGC TCAAATGGAG CCATCGCCTG CGAAACCTCC CCAGGTAGAA 
GCACCCACTT ACCCCCAGCC TCCCCAAAGG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT 
CCCACGGGGC CTGCCCCTCC CACGGAGCCT GCCCCTCCCA CGGGGCTTGC CGAGATGGAA 
CCTTCTCCCA CGGAGCCTTC CCAGAAGGAA CCACCTCCCA GTATGGAGCC TCCCTGCCCC 
GAGGAGCTGC CTCAGGCCGA GCCACCTCCT ATGGAGGATT GTCAGAAGGA GCTGCCTTCT 
CCCGTGGAGC CCGCTCAGAT TGAGGTTGCT CAGACGGCCC CTACGCAGGT TCAGGAGGAG 
CCCCCTCCTG TCTCGGAGCC ACCTCGGGTG AAGCCAACCA AAAGATCATC TCTCCGGAAA 
GACAGAGCAG AGAAGGAGCT GAGCCTGCTG AGTGAGATGG CGCGGCAGGA GCAGGTCCTC 
ATGGGGGTTG GCTTGGTGCC TGTTAGAGAC AGCAAGCTTC TGAAGGGAAA CAAGAGCGCC 
CAGGACCCCC CAGCCCCACC GTCACCATCG CCAAAGGGAA ACTCGAGGGA AGAGACACCC 
AAGGACCAAG AAATGGTCTC TGATGGGGAA GGAACTATAG TATTCCCTCT CAAGAAAGGA 
GGACCAGAGG AAGCTGGAGA GAGTCCAGCT GAGTTGGCTG CTCTCAAGGA GTCTGCCCGT 
GTTTCATCCT CTGAACAAAA CTCAGCCATG CCAGAGGGTG GAGCATCACA CAGCAAGTGT 
CAGACTGGCT CCTCTGGGCT TTGTGACGTG GACACTGAGC AGAAGACAGA TACTGTCCCC 
ATGAAAGACT CCGCAGCAGA GCCAGTGTCC CCTCCTACCC CAACAGTGGA CCGTGACGCA 
GGGTCACCAG CTGTAGTGGC CTCCCCTCCT ATCACGTTGG CTGAAAACGA GTCTCAGGAA 
ATTGATGAAG ATGAAGGCAT CCATAGCCAT GATGGAAGTG ACCTGAGTGA CAACATGTCT 
GAGGGGAGTG ACGACTCAGG ACTGCACGGG GCTCGGCCGA CACCACCAGA AGCTACGTCA 
AAAAATGGGA AGGCAGGGTT GGCTGGTAAA GTGACTGAGG GAGAGTTTGT GTGTATTTTC 
TGTGATCGTT CTTTTAGAAA GGAAAAAGAT TATAGCAAAC ACCTCAATCG CCACTTGGTG 
AATGTGTACT TCCTAGAAGA AGCAGCTGAG GAGCAGGAGG AGCAGGAGGA GCGGGAGGAG 
CAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overlapping Activities of Two Neuronal Splicing Factors Switch the GABA Effect from Excitatory to Inhibitory by Regulating REST.
Cell reports27(3)860-871.e8(2019 04)
Nakano Y,Wiechert S,Bánfi B

RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and
The Journal of biological chemistry293(49)19078-19091(2018 12)
Zhang J,Chen SR,Chen H,Pan HL

Sertraline, chlorprothixene, and chlorpromazine characteristically interact with the REST-binding site of the corepressor mSin3, showing medulloblastoma cell growth inhibitory activities.
Scientific reports8(1)13763(2018 09)
Kurita JI,Hirao Y,Nakano H,Fukunishi Y,Nishimura Y

Defects in the Alternative Splicing-Dependent Regulation of REST Cause Deafness.
Cell174(3)536-548.e21(2018 07)
Nakano Y,Kelly MC,Rehman AU,Boger ET,Morell RJ,Kelley MW,Friedman TB,Bánfi B

The Epigenetic Factor Landscape of Developing Neocortex Is Regulated by Transcription Factors Pax6→ Tbr2→ Tbr1.
Frontiers in neuroscience12571(2018)
Elsen GE,Bedogni F,Hodge RD,Bammler TK,MacDonald JW,Lindtner S,Rubenstein JLR,Hevner RF

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Rest cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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