Myh6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh6
Entrez Gene ID	17888
Full Name	myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms	A830009F23Rik,AA517445,Myhc-a,Myhca,alpha-MHC
General protein information	Preferred Names myosin, heavy polypeptide 6, cardiac muscle, alpha Names myosin-6 Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha) alpha cardiac MHC alpha myosin cardiac myosin heavy chain alpha isoform cardiomyopathy, hypertrophic 1 myHC-alpha myosin heavy chain 6 myosin heavy chain, cardiac muscle, adult
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C2-C3\|14 28.01 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010856.4	NP_034986.1	myosin-6
XM_006518677.1	XP_006518740.1	myosin-6 isoform X1
NM_001164171.1	NP_001157643.1	myosin-6
XM_006518678.3	XP_006518741.1	myosin-6 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH6	XP_003952494.1
Gallus gallus (chicken)	MYH15	NP_990097.1
Canis lupus familiaris (dog)	MYH6	XP_003435121.2
Mus musculus (house mouse)	Myh6	NP_034986.1
Rattus norvegicus (Norway rat)	Myh6	NP_058935.2
Homo sapiens (human)	MYH6	NP_002462.2
Macaca mulatta (Rhesus monkey)	MYH6	XP_001102827.2
Bos taurus (cattle)	MYH6	XP_002690549.1
Danio rerio (zebrafish)	myh6	NP_942118.1
Xenopus tropicalis (tropical clawed frog)	myh6	XP_004910815.1

Gene Ontology
Bibliography

Related articles in PubMed

Stimulation of β-adrenoceptors up-regulates cardiac expression of galectin-3 and BIM through the Hippo signalling pathway.
Zhao WB, Lu Q, Nguyen MN, Su Y, Ziemann M, Wang LN, Kiriazis H, Puthalakath H, Sadoshima J, Hu HY, Du XJ
British journal of pharmacology176(14)2465-2481(2019 Jul)

New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to β-adrenergic stimulation-induced cardiac fibrosis.
Takahara S, Inoue SI, Miyagawa-Tomita S, Matsuura K, Nakashima Y, Niihori T, Matsubara Y, Saiki Y, Aoki Y
EBioMedicine4243-53(2019 Apr)

Myocardial-specific ablation of
Cho E, Kang H, Kang DK, Lee Y
The Journal of biological chemistry294(13)4981-4996(2019 03)

Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4.
Da Silva F, Massa F, Motamedi FJ, Vidal V, Rocha AS, Gregoire EP, Cai CL, Wagner KD, Schedl A
Developmental biology441(1)42-51(2018 09)

Neddylation mediates ventricular chamber maturation through repression of Hippo signaling.
Zou J, Ma W, Li J, Littlejohn R, Zhou H, Kim IM, Fulton DJR, Chen W, Weintraub NL, Zhou J, Su H
Proceedings of the National Academy of Sciences of the United States of America115(17)E4101-E4110(2018 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Myh6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19977	NM_010856.4 Latest version!	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu19977	NM_001164171.1 Latest version!	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu49762	XM_006518677.1 Latest version!	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu49762	XM_006518678.3 Latest version!	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh6 ( NM_010856.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034986.1 Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19977
Clone ID Related Accession (Same CDS sequence)	NM_010856.4 , NM_001164171.1
Accession Version	NM_010856.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	myosin-6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT025533.11. On Aug 13, 2009 this sequence version replaced NM_010856.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC110700.1, M76598.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849390 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGAAGA TGCACGACGA GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034986.1
CDS	139..5955
Translation	MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKVVSREGGKVTAETENGKTVTIKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKKMHDEE

Target ORF information:

RefSeq Version	NM_010856.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010856.4

ORF Insert Sequence:

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGAAGA TGCACGACGA GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh6 ( NM_001164171.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157643.1 Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant 1, mRNA.

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CloneID	OMu19977
Clone ID Related Accession (Same CDS sequence)	NM_010856.4 , NM_001164171.1
Accession Version	NM_001164171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	myosin-6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT025533.11. Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849390 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGAAGA TGCACGACGA GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157643.1
CDS	203..6019
Translation	MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKVVSREGGKVTAETENGKTVTIKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKKMHDEE

Target ORF information:

RefSeq Version	NM_001164171.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164171.1

ORF Insert Sequence:

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGAAGA TGCACGACGA GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh6 ( XM_006518677.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518740.1 Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant X1, mRNA.

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CloneID	OMu49762
Clone ID Related Accession (Same CDS sequence)	XM_006518677.1 , XM_006518678.3
Accession Version	XM_006518677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	myosin-6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGCAGA AGATGCACGA CGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518740.1
CDS	241..6060
Translation	MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKVVSREGGKVTAETENGKTVTIKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKQKMHDEE

Target ORF information:

RefSeq Version	XM_006518677.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518677.1

ORF Insert Sequence:

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGCAGA AGATGCACGA CGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh6 ( XM_006518678.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518741.1 Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant X2, mRNA.

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CloneID	OMu49762
Clone ID Related Accession (Same CDS sequence)	XM_006518677.1 , XM_006518678.3
Accession Version	XM_006518678.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	myosin-6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGCAGA AGATGCACGA CGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518741.1
CDS	299..6118
Translation	MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKVVSREGGKVTAETENGKTVTIKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKQKMHDEE

Target ORF information:

RefSeq Version	XM_006518678.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518678.3

ORF Insert Sequence:

ATGACGGACG CCCAGATGGC TGACTTCGGG GCAGCAGCCC AGTACCTCCG AAAGTCAGAG 
AAGGAACGCC TAGAGGCCCA GACCCGGCCC TTTGACATCC GCACGGAGTG CTTCGTGCCT 
GATGACAAGG AGGAGTATGT TAAGGCCAAG GTCGTGTCCC GGGAAGGGGG CAAAGTCACT 
GCGGAAACTG AAAACGGAAA GACGGTGACC ATAAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTGTACA ACCTCAAGGA GCGCTACGCG GCCTGGATGA TCTATACCTA CTCAGGCCTC 
TTCTGCGTCA CCGTCAACCC CTATAAGTGG CTGCCTGTGT ACAATGCGGA AGTGGTGGCC 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCTCCTCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AATCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCGGGGA AGACTGTGAA CACAAAACGT GTCATCCAGT ACTTTGCCAG CATTGCAGCC 
ATAGGGGACC GTAGCAAGAA GGAAAATCCT AATGCAAACA AGGGCACCCT GGAGGACCAG 
ATTATCCAGG CTAACCCCGC TCTGGAGGCC TTCGGCAACG CCAAGACTGT CCGGAATGAC 
AACTCCTCCC GCTTTGGGAA ATTCATCAGG ATCCACTTTG GAGCTACTGG AAAGCTGGCT 
TCTGCAGACA TAGAGACCTA CCTTCTGGAG AAGTCCCGGG TGATCTTCCA GCTAAAGGCT 
GAGAGGAACT ACCACATCTT CTACCAGATC CTGTCCAACA AGAAGCCGGA GCTGCTGGAC 
ATGCTGCTGG TCACCAACAA CCCATACGAC TACGCCTTCG TCTCTCAGGG AGAGGTGTCC 
GTGGCCTCCA TTGATGACTC TGAGGAGCTC TTGGCCACTG ATAGTGCCTT TGATGTGCTG 
AGCTTCACGG CAGAGGAGAA GGCTGGTGTC TACAAGCTGA CAGGGGCCAT CATGCACTAC 
GGAAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CGGAGCCTGA TGGCACAGAA 
GATGCTGACA AATCTGCCTA CCTTATGGGG CTGAACTCAG CTGACCTGCT CAAGGGCCTG 
TGTCACCCTC GGGTGAAGGT GGGGAACGAG TATGTCACCA AGGGGCAGAG TGTACAGCAA 
GTGTACTATT CCATCGGGGC ACTGGCCAAG TCAGTGTACG AGAAGATGTT CAACTGGATG 
GTGACACGCA TCAACGCAAC CCTGGAGACC AAGCAGCCGC GCCAGTACTT CATAGGTGTC 
CTGGACATTG CCGGCTTTGA GATCTTCGAT TTCAACAGCT TTGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAGCTGCA GCAGTTCTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAGGGCAT TGAGTGGGAG TTTATCGACT TCGGCATGGA CCTGCAGGCC 
TGCATCGACC TCATCGAGAA GCCCATGGGC ATCATGTCCA TCCTCGAGGA GGAGTGCATG 
TTCCCCAAGG CCTCAGACAT GACCTTCAAG GCCAAGCTGT ATGACAACCA CCTGGGCAAA 
TCCAACAACT TCCAGAAGCC TCGCAATGTC AAGGGGAAGC AGGAAGCCCA CTTCTCCTTG 
GTCCACTATG CTGGCACCGT GGACTACAAC ATTATGGGCT GGCTGGAAAA GAACAAGGAC 
CCACTCAATG AGACGGTGGT GGGTTTGTAC CAGAAGTCCT CCCTCAAGCT CATGGCTACA 
CTCTTCTCTA CCTATGCTTC TGCTGATACC GGTGACAGTG GTAAAGGCAA AGGAGGCAAG 
AAGAAAGGCT CATCCTTCCA AACAGTGTCT GCTCTCCACC GGGAAAATCT GAACAAGCTG 
ATGACAAACC TGAAGACCAC CCACCCTCAC TTTGTGCGCT GCATCATTCC CAACGAGCGA 
AAGGCTCCAG GGGTGATGGA CAACCCCCTG GTCATGCACC AGCTGCGATG CAATGGCGTG 
CTGGAGGGTA TCCGCATCTG CAGGAAGGGC TTCCCCAACC GCATTCTCTA TGGGGACTTC 
CGGCAGAGGT ATCGCATCCT GAACCCAGCA GCCATCCCTG AGGGGCAATT CATTGATAGC 
AGGAAAGGGG CTGAGAAACT GCTGGGCTCC CTGGACATTG ACCACAACCA ATACAAGTTT 
GGCCACACCA AGGTGTTCTT CAAGGCGGGC CTGCTGGGGC TGCTCGAGGA GATGCGAGAT 
GAGAGGCTGA GCCGTATCAT CACCAGAATC CAGGCCCAGG CCCGAGGGCA GCTCATGCGC 
ATTGAGTTCA AGAAGATAGT GGAACGCAGG GATGCCCTGC TGGTTATCCA GTGGAACATT 
CGGGCCTTCA TGGGGGTCAA GAATTGGCCA TGGATGAAGC TCTACTTCAA GATCAAACCG 
CTGCTGAAGA GCGCAGAGAC GGAGAAGGAG ATGGCCAACA TGAAGGAGGA GTTTGGGCGA 
GTCAAAGATG CACTGGAGAA GTCTGAGGCT CGCCGCAAGG AGCTGGAGGA GAAGATGGTG 
TCCCTGCTGC AGGAGAAGAA TGACCTACAG CTCCAAGTGC AGGCGGAACA AGACAACCTC 
AATGATGCAG AGGAGCGCTG TGACCAGCTG ATCAAGAACA AGATCCAGCT GGAGGCCAAG 
GTGAAGGAGA TGACCGAGAG GCTGGAGGAC GAGGAGGAGA TGAACGCCGA GCTCACTGCC 
AAGAAGCGCA AGCTGGAAGA TGAGTGCTCA GAGCTCAAGA AGGATATTGA TGACCTGGAG 
CTGACGCTGG CCAAGGTGGA AAAGGAAAAG CATGCAACAG AGAACAAGGT TAAAAACCTA 
ACAGAGGAGA TGGCTGGGCT GGATGAAATC ATTGCCAAGC TGACCAAAGA GAAGAAAGCT 
CTGCAAGAAG CCCACCAGCA AGCCCTCGAT GACCTGCAGG CTGAAGAAGA CAAGGTCAAC 
ACGCTGACCA AGTCCAAAGT CAAGCTGGAG CAGCAGGTGG ATGATCTGGA GGGATCCCTG 
GAGCAGGAGA AGAAAGTGCG CATGGACCTA GAGCGAGCCA AGCGGAAGCT GGAGGGAGAC 
CTGAAGCTGA CCCAGGAGAG CATCATGGAC CTGGAGAATG ACAAGCTTCA GCTGGAAGAA 
AAGCTCAAGA AGAAAGAGTT CGACATCAGT CAGCAGAACA GTAAAATTGA GGACGAGCAG 
GCCCTGGCTC TTCAGCTGCA GAAGAAACTG AAGGAAAACC AGGCACGCAT CGAGGAGCTG 
GAGGAGGAGC TGGAGGCAGA GCGCACAGCC CGGGCTAAGG TGGAGAAGCT GCGCTCTGAC 
CTGTCCCGGG AGCTGGAGGA GATCAGTGAG AGGCTGGAGG AGGCAGGCGG GGCCACATCC 
GTGCAGATAG AGATGAATAA GAAGCGCGAG GCCGAGTTCC AGAAGATGCG GCGGGACCTG 
GAGGAGGCCA CGCTGCAGCA CGAGGCCACG GCGGCGGCCC TGCGCAAGAA GCATGCTGAC 
AGCGTGGCGG AGCTGGGCGA GCAGATCGAC AACCTCCAGC GGGTGAAGCA GAAGCTGGAG 
AAAGAGAAGA GCGAGTTCAA GCTGGAGCTG GATGACGTCA CCTCCAACAT GGAGCAGATC 
ATCAAGGCCA AGGCCAACCT GGAGAAAGTG TCCCGGACAC TGGAGGACCA GGCCAATGAG 
TACCGCGTGA AGCTGGAAGA AGCCCAGCGC TCCCTCAATG ACTTCACCAC ACAGCGAGCC 
AAGCTGCAGA CAGAGAACGG GGAGTTGGCT AGGCAACTGG AAGAAAAGGA GGCATTGATT 
TCCCAGCTGA CCCGAGGCAA GCTCTCCTAC ACCCAGCAGA TGGAGGACCT CAAGAGGCAA 
CTGGAGGAGG AAGGCAAGGC CAAGAACGCC CTGGCCCACG CACTGCAATC ATCCCGGCAT 
GACTGTGACC TGCTGAGGGA ACAGTATGAA GAAGAAATGG AGGCCAAGGC TGAGCTACAG 
CGTGTCCTGT CCAAGGCCAA CTCAGAGGTG GCCCAGTGGA GGACCAAGTA TGAGACGGAT 
GCCATACAGA GGACGGAGGA GCTGGAGGAA GCCAAGAAGA AGCTGGCTCA GAGGCTGCAG 
GATGCAGAGG AGGCAGTGGA GGCCGTCAAC GCCAAGTGTT CCTCCCTGGA GAAGACCAAG 
CACAGGCTGC AGAATGAGAT CGAGGACCTG ATGGTGGACG TGGAGCGCTC CAATGCCGCC 
GCCGCAGCCC TGGACAAGAA GCAGAGGAAC TTTGACAAGA TCCTGGCTGA GTGGAAGCAG 
AAGTATGAGG AGTCGCAGTC AGAGCTGGAG TCTTCCCAGA AGGAGGCGCG CTCCCTGAGC 
ACAGAGCTCT TCAAGCTCAA GAACGCCTAT GAGGAGTCTC TGGAGCACCT GGAGACCTTC 
AAGCGGGAGA ACAAGAACCT CCAGGAGGAG ATCTCAGACC TGACTGAACA GCTGGGAGAA 
GGGGGGAAAA ACGTGCACGA GCTGGAGAAG ATCCGCAAAC AGCTGGAGGT GGAGAAGCTG 
GAGCTGCAGT CAGCCCTGGA GGAGGCTGAG GCCTCCCTGG AGCACGAGGA GGGCAAGATC 
CTCCGTGCCC AGCTGGAGTT CAACCAGATC AAGGCAGAGA TCGAAAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GGCCAAGCGC AACCACCTGC GGATGGTGGA CTCCCTGCAG 
ACCTCCCTGG ATGCGGAGAC ACGCAGCCGC AATGAGGCCC TGCGGGTGAA GAAGAAGATG 
GAGGGCGACC TCAACGAGAT GGAGATCCAG CTCAGCCAGG CCAATAGAAT AGCCTCAGAG 
GCACAGAAAC ACCTGAAGAA TTCTCAAGCT CACTTGAAGG ACACCCAGCT CCAGCTGGAT 
GATGCTGTCC ATGCCAATGA CGACCTGAAG GAGAACATCG CCATCGTGGA ACGGCGCAAC 
AACCTGCTGC AGGCGGAGCT GGAGGAGCTG CGGGCTGTGG TGGAGCAGAC GGAGCGGTCT 
CGGAAGCTGG CAGAGCAGGA GCTGATTGAG ACCAGCGAGC GGGTGCAGCT GCTGCACTCG 
CAGAACACCA GCCTCATCAA CCAGAAGAAG AAGATGGAGT CAGACCTGAC CCAACTCCAG 
ACAGAAGTAG AGGAGGCAGT GCAGGAGTGT AGGAACGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACAGATG CCGCAATGAT GGCTGAGGAG CTGAAGAAGG AGCAGGACAC CAGCGCCCAC 
CTGGAGCGCA TGAAGAAGAA CATGGAGCAG ACCATCAAGG ACTTGCAGCA CCGTCTGGAC 
GAGGCAGAGC AGATCGCCCT CAAGGGCGGC AAGAAGCAGC TGCAGAAGCT GGAGGCCCGG 
GTCCGGGAGC TGGAGAATGA GCTGGAGGCT GAGCAGAAGC GCAATGCAGA GTCGGTGAAG 
GGCATGAGGA AGAGCGAGCG GCGCATCAAG GAGCTCACCT ACCAGACAGA GGAAGACAAG 
AAGAACTTAA TGCGGCTGCA GGACCTGGTG GACAAGCTAC AGTTGAAGGT GAAGGCCTAC 
AAGCGCCAGG CTGAGGAGGC GGAGGAGCAG GCCAACACCA ACCTGTCCAA GTTCCGCAAG 
GTGCAGCACG AGCTGGATGA GGCGGAGGAG AGGGCGGACA TCGCCGAGTC CCAGGTCAAC 
AAGCTGCGGG CCAAGAGCCG GGACATTGGT GCCAAGCAGA AGATGCACGA CGAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stimulation of β-adrenoceptors up-regulates cardiac expression of galectin-3 and BIM through the Hippo signalling pathway.
British journal of pharmacology176(14)2465-2481(2019 Jul)
Zhao WB,Lu Q,Nguyen MN,Su Y,Ziemann M,Wang LN,Kiriazis H,Puthalakath H,Sadoshima J,Hu HY,Du XJ

New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to β-adrenergic stimulation-induced cardiac fibrosis.
EBioMedicine4243-53(2019 Apr)
Takahara S,Inoue SI,Miyagawa-Tomita S,Matsuura K,Nakashima Y,Niihori T,Matsubara Y,Saiki Y,Aoki Y

Myocardial-specific ablation of
The Journal of biological chemistry294(13)4981-4996(2019 03)
Cho E,Kang H,Kang DK,Lee Y

Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4.
Developmental biology441(1)42-51(2018 09)
Da Silva F,Massa F,Motamedi FJ,Vidal V,Rocha AS,Gregoire EP,Cai CL,Wagner KD,Schedl A

Neddylation mediates ventricular chamber maturation through repression of Hippo signaling.
Proceedings of the National Academy of Sciences of the United States of America115(17)E4101-E4110(2018 04)
Zou J,Ma W,Li J,Littlejohn R,Zhou H,Kim IM,Fulton DJR,Chen W,Weintraub NL,Zhou J,Su H

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Myh6 cDNA ORF clone, Mus musculus(house mouse)

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