PPM1D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPM1D
Entrez Gene ID	8493
Full Name	protein phosphatase, Mg2+/Mn2+ dependent 1D
Synonyms	IDDGIP,PP2C-DELTA,WIP1
General protein information	Preferred Names protein phosphatase, Mg2+/Mn2+ dependent 1D Names protein phosphatase 1D protein phosphatase 1D magnesium-dependent, delta isoform protein phosphatase 2C delta isoform protein phosphatase Wip1 wild-type p53-induced phosphatase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q23.2
Summary	The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008].
Disorder MIM:	605100
Disorder Html:	Intellectual developmental disorder with gastrointestinal difficulties and high pain threshold, 617450 (3); Breast cancer, somatic, 114480 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003620.4	NP_003611.1	protein phosphatase 1D
NM_003620.3	NP_003611.1	protein phosphatase 1D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPM1D	XP_852759.2
Rattus norvegicus (Norway rat)	Ppm1d	NP_001099295.2
Danio rerio (zebrafish)	ppm1da	NP_957384.2
Macaca mulatta (Rhesus monkey)	PPM1D	XP_001110891.1
Mus musculus (house mouse)	Ppm1d	NP_058606.3
Xenopus tropicalis (tropical clawed frog)	ppm1d	XP_002933883.2
Homo sapiens (human)	PPM1D	NP_003611.1
Pan troglodytes (chimpanzee)	PPM1D	NP_001233479.1
Danio rerio (zebrafish)	ppm1db	NP_001007341.1
Gallus gallus (chicken)	PPM1D	XP_415890.3
Bos taurus (cattle)	PPM1D	NP_001178373.1

Gene Ontology
Bibliography

Related articles in PubMed

Detectible mosaic truncating PPM1D mutations, age and breast cancer risk.
Machiela MJ, Myers TA, Lyons CJ, Koster R, Figg WD Jr, Colli LM, Jessop L, Ahearn TU, Freedman ND, García-Closas M, Chanock SJ
Journal of human genetics64(6)545-550(2019 Jun)

The Regulation of Trophoblastic p53 Homeostasis by the p38-Wip1 Feedback Loop is Disturbed in Placentas from Pregnancies Complicated by Preeclampsia.
Tan B, Tong C, Yuan Y, Xu P, Wen L, Zhang C, Zheng Y, Lin L, Zhu F, Gui S, Wang L, Gao R, Li J, Qi H, Baker PN
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology52(2)315-335(2019)

Absence of EIF1AX, PPM1D, and CHEK2 mutations reported in Thyroid Cancer Genome Atlas (TCGA) in a large series of thyroid cancer.
Alzahrani AS, Murugan AK, Qasem E, Alswailem MM, AlGhamdi B, Moria Y, Al-Hindi H
Endocrine63(1)94-100(2019 01)

Wip1 knockout inhibits neurogenesis by affecting the Wnt/β-catenin signaling pathway in focal cerebral ischemia in mice.
Qiu CW, Liu ZY, Hou K, Liu SY, Hu YX, Zhang L, Zhang FL, Lv KY, Kang Q, Hu WY, Ma N, Jiao Y, Bai WJ, Xiao ZC
Experimental neurology30944-53(2018 11)

Kahn JD, Miller PG, Silver AJ, Sellar RS, Bhatt S, Gibson C, McConkey M, Adams D, Mar B, Mertins P, Fereshetian S, Krug K, Zhu H, Letai A, Carr SA, Doench J, Jaiswal S, Ebert BL
Blood132(11)1095-1105(2018 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPM1D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPM1D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20880	NM_003620.4 Latest version!	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1D (PPM1D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu20880	NM_003620.3	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1D (PPM1D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPM1D ( NM_003620.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003611.1 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1D (PPM1D), mRNA.

Price & Availability↑

CloneID	OHu20880
Clone ID Related Accession (Same CDS sequence)	NM_003620.4 , NM_003620.3
Accession Version	NM_003620.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1567958400000
Organism	Homo sapiens(human)
Product	protein phosphatase 1D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC060877.1, U78305.1, AC110602.8 and BU616782.1. On Aug 31, 2019 this sequence version replaced NM_003620.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312902.1, SRR1660803.21966.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGC TGTACTCGCT GGGAGTGAGC GTCTTCTCCG ACCAGGGCGG GAGGAAGTAC 
ATGGAGGACG TTACTCAAAT CGTTGTGGAG CCCGAACCGA CGGCTGAAGA AAAGCCCTCG 
CCGCGGCGGT CGCTGTCTCA GCCGTTGCCT CCGCGGCCGT CGCCGGCCGC CCTTCCCGGC 
GGCGAAGTCT CGGGGAAAGG CCCAGCGGTG GCAGCCCGAG AGGCTCGCGA CCCTCTCCCG 
GACGCCGGGG CCTCGCCGGC ACCTAGCCGC TGCTGCCGCC GCCGTTCCTC CGTGGCCTTT 
TTCGCCGTGT GCGACGGGCA CGGCGGGCGG GAGGCGGCAC AGTTTGCCCG GGAGCACTTG 
TGGGGTTTCA TCAAGAAGCA GAAGGGTTTC ACCTCGTCCG AGCCGGCTAA GGTTTGCGCT 
GCCATCCGCA AAGGCTTTCT CGCTTGTCAC CTTGCCATGT GGAAGAAACT GGCGGAATGG 
CCAAAGACTA TGACGGGTCT TCCTAGCACA TCAGGGACAA CTGCCAGTGT GGTCATCATT 
CGGGGCATGA AGATGTATGT AGCTCACGTA GGTGACTCAG GGGTGGTTCT TGGAATTCAG 
GATGACCCGA AGGATGACTT TGTCAGAGCT GTGGAGGTGA CACAGGACCA TAAGCCAGAA 
CTTCCCAAGG AAAGAGAACG AATCGAAGGA CTTGGTGGGA GTGTAATGAA CAAGTCTGGG 
GTGAATCGTG TAGTTTGGAA ACGACCTCGA CTCACTCACA ATGGACCTGT TAGAAGGAGC 
ACAGTTATTG ACCAGATTCC TTTTCTGGCA GTAGCAAGAG CACTTGGTGA TTTGTGGAGC 
TATGATTTCT TCAGTGGTGA ATTTGTGGTG TCACCTGAAC CAGACACAAG TGTCCACACT 
CTTGACCCTC AGAAGCACAA GTATATTATA TTGGGGAGTG ATGGACTTTG GAATATGATT 
CCACCACAAG ATGCCATCTC AATGTGCCAG GACCAAGAGG AGAAAAAATA CCTGATGGGT 
GAGCATGGAC AATCTTGTGC CAAAATGCTT GTGAATCGAG CATTGGGCCG CTGGAGGCAG 
CGTATGCTCC GAGCAGATAA CACTAGTGCC ATAGTAATCT GCATCTCTCC AGAAGTGGAC 
AATCAGGGAA ACTTTACCAA TGAAGATGAG TTATACCTGA ACCTGACTGA CAGCCCTTCC 
TATAATAGTC AAGAAACCTG TGTGATGACT CCTTCCCCAT GTTCTACACC ACCAGTCAAG 
TCACTGGAGG AGGATCCATG GCCAAGGGTG AATTCTAAGG ACCATATACC TGCCCTGGTT 
CGTAGCAATG CCTTCTCAGA GAATTTTTTA GAGGTTTCAG CTGAGATAGC TCGAGAGAAT 
GTCCAAGGTG TAGTCATACC CTCAAAAGAT CCAGAACCAC TTGAAGAAAA TTGCGCTAAA 
GCCCTGACTT TAAGGATACA TGATTCTTTG AATAATAGCC TTCCAATTGG CCTTGTGCCT 
ACTAATTCAA CAAACACTGT CATGGACCAA AAAAATTTGA AGATGTCAAC TCCTGGCCAA 
ATGAAAGCCC AAGAAATTGA AAGAACCCCT CCAACAAACT TTAAAAGGAC ATTAGAAGAG 
TCCAATTCTG GCCCCCTGAT GAAGAAGCAT AGACGAAATG GCTTAAGTCG AAGTAGTGGT 
GCTCAGCCTG CAAGTCTCCC CACAACCTCA CAGCGAAAGA ACTCTGTTAA ACTCACCATG 
CGACGCAGAC TTAGGGGCCA GAAGAAAATT GGAAATCCTT TACTTCATCA ACACAGGAAA 
ACTGTTTGTG TTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003611.1
CDS	223..2040
Translation	MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPTAEEKPSPRRSLSQPLPPRPSPAALPGGEVSGKGPAVAAREARDPLPDAGASPAPSRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHKPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLEEDPWPRVNSKDHIPALVRSNAFSENFLEVSAEIARENVQGVVIPSKDPEPLEENCAKALTLRIHDSLNNSLPIGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRKTVCVC

Target ORF information:

RefSeq Version	NM_003620.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1D (PPM1D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003620.4

ORF Insert Sequence:

ATGGCGGGGC TGTACTCGCT GGGAGTGAGC GTCTTCTCCG ACCAGGGCGG GAGGAAGTAC 
ATGGAGGACG TTACTCAAAT CGTTGTGGAG CCCGAACCGA CGGCTGAAGA AAAGCCCTCG 
CCGCGGCGGT CGCTGTCTCA GCCGTTGCCT CCGCGGCCGT CGCCGGCCGC CCTTCCCGGC 
GGCGAAGTCT CGGGGAAAGG CCCAGCGGTG GCAGCCCGAG AGGCTCGCGA CCCTCTCCCG 
GACGCCGGGG CCTCGCCGGC ACCTAGCCGC TGCTGCCGCC GCCGTTCCTC CGTGGCCTTT 
TTCGCCGTGT GCGACGGGCA CGGCGGGCGG GAGGCGGCAC AGTTTGCCCG GGAGCACTTG 
TGGGGTTTCA TCAAGAAGCA GAAGGGTTTC ACCTCGTCCG AGCCGGCTAA GGTTTGCGCT 
GCCATCCGCA AAGGCTTTCT CGCTTGTCAC CTTGCCATGT GGAAGAAACT GGCGGAATGG 
CCAAAGACTA TGACGGGTCT TCCTAGCACA TCAGGGACAA CTGCCAGTGT GGTCATCATT 
CGGGGCATGA AGATGTATGT AGCTCACGTA GGTGACTCAG GGGTGGTTCT TGGAATTCAG 
GATGACCCGA AGGATGACTT TGTCAGAGCT GTGGAGGTGA CACAGGACCA TAAGCCAGAA 
CTTCCCAAGG AAAGAGAACG AATCGAAGGA CTTGGTGGGA GTGTAATGAA CAAGTCTGGG 
GTGAATCGTG TAGTTTGGAA ACGACCTCGA CTCACTCACA ATGGACCTGT TAGAAGGAGC 
ACAGTTATTG ACCAGATTCC TTTTCTGGCA GTAGCAAGAG CACTTGGTGA TTTGTGGAGC 
TATGATTTCT TCAGTGGTGA ATTTGTGGTG TCACCTGAAC CAGACACAAG TGTCCACACT 
CTTGACCCTC AGAAGCACAA GTATATTATA TTGGGGAGTG ATGGACTTTG GAATATGATT 
CCACCACAAG ATGCCATCTC AATGTGCCAG GACCAAGAGG AGAAAAAATA CCTGATGGGT 
GAGCATGGAC AATCTTGTGC CAAAATGCTT GTGAATCGAG CATTGGGCCG CTGGAGGCAG 
CGTATGCTCC GAGCAGATAA CACTAGTGCC ATAGTAATCT GCATCTCTCC AGAAGTGGAC 
AATCAGGGAA ACTTTACCAA TGAAGATGAG TTATACCTGA ACCTGACTGA CAGCCCTTCC 
TATAATAGTC AAGAAACCTG TGTGATGACT CCTTCCCCAT GTTCTACACC ACCAGTCAAG 
TCACTGGAGG AGGATCCATG GCCAAGGGTG AATTCTAAGG ACCATATACC TGCCCTGGTT 
CGTAGCAATG CCTTCTCAGA GAATTTTTTA GAGGTTTCAG CTGAGATAGC TCGAGAGAAT 
GTCCAAGGTG TAGTCATACC CTCAAAAGAT CCAGAACCAC TTGAAGAAAA TTGCGCTAAA 
GCCCTGACTT TAAGGATACA TGATTCTTTG AATAATAGCC TTCCAATTGG CCTTGTGCCT 
ACTAATTCAA CAAACACTGT CATGGACCAA AAAAATTTGA AGATGTCAAC TCCTGGCCAA 
ATGAAAGCCC AAGAAATTGA AAGAACCCCT CCAACAAACT TTAAAAGGAC ATTAGAAGAG 
TCCAATTCTG GCCCCCTGAT GAAGAAGCAT AGACGAAATG GCTTAAGTCG AAGTAGTGGT 
GCTCAGCCTG CAAGTCTCCC CACAACCTCA CAGCGAAAGA ACTCTGTTAA ACTCACCATG 
CGACGCAGAC TTAGGGGCCA GAAGAAAATT GGAAATCCTT TACTTCATCA ACACAGGAAA 
ACTGTTTGTG TTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPM1D ( NM_003620.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003611.1 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1D (PPM1D), mRNA.

Price & Availability↑

CloneID	OHu20880
Clone ID Related Accession (Same CDS sequence)	NM_003620.4 , NM_003620.3
Accession Version	NM_003620.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1560787200000
Organism	Homo sapiens(human)
Product	protein phosphatase 1D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC060877.1, U78305.1, AC110602.8 and BU616782.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2010 this sequence version replaced NM_003620.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060877.1, SRR1660803.21966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGC TGTACTCGCT GGGAGTGAGC GTCTTCTCCG ACCAGGGCGG GAGGAAGTAC 
ATGGAGGACG TTACTCAAAT CGTTGTGGAG CCCGAACCGA CGGCTGAAGA AAAGCCCTCG 
CCGCGGCGGT CGCTGTCTCA GCCGTTGCCT CCGCGGCCGT CGCCGGCCGC CCTTCCCGGC 
GGCGAAGTCT CGGGGAAAGG CCCAGCGGTG GCAGCCCGAG AGGCTCGCGA CCCTCTCCCG 
GACGCCGGGG CCTCGCCGGC ACCTAGCCGC TGCTGCCGCC GCCGTTCCTC CGTGGCCTTT 
TTCGCCGTGT GCGACGGGCA CGGCGGGCGG GAGGCGGCAC AGTTTGCCCG GGAGCACTTG 
TGGGGTTTCA TCAAGAAGCA GAAGGGTTTC ACCTCGTCCG AGCCGGCTAA GGTTTGCGCT 
GCCATCCGCA AAGGCTTTCT CGCTTGTCAC CTTGCCATGT GGAAGAAACT GGCGGAATGG 
CCAAAGACTA TGACGGGTCT TCCTAGCACA TCAGGGACAA CTGCCAGTGT GGTCATCATT 
CGGGGCATGA AGATGTATGT AGCTCACGTA GGTGACTCAG GGGTGGTTCT TGGAATTCAG 
GATGACCCGA AGGATGACTT TGTCAGAGCT GTGGAGGTGA CACAGGACCA TAAGCCAGAA 
CTTCCCAAGG AAAGAGAACG AATCGAAGGA CTTGGTGGGA GTGTAATGAA CAAGTCTGGG 
GTGAATCGTG TAGTTTGGAA ACGACCTCGA CTCACTCACA ATGGACCTGT TAGAAGGAGC 
ACAGTTATTG ACCAGATTCC TTTTCTGGCA GTAGCAAGAG CACTTGGTGA TTTGTGGAGC 
TATGATTTCT TCAGTGGTGA ATTTGTGGTG TCACCTGAAC CAGACACAAG TGTCCACACT 
CTTGACCCTC AGAAGCACAA GTATATTATA TTGGGGAGTG ATGGACTTTG GAATATGATT 
CCACCACAAG ATGCCATCTC AATGTGCCAG GACCAAGAGG AGAAAAAATA CCTGATGGGT 
GAGCATGGAC AATCTTGTGC CAAAATGCTT GTGAATCGAG CATTGGGCCG CTGGAGGCAG 
CGTATGCTCC GAGCAGATAA CACTAGTGCC ATAGTAATCT GCATCTCTCC AGAAGTGGAC 
AATCAGGGAA ACTTTACCAA TGAAGATGAG TTATACCTGA ACCTGACTGA CAGCCCTTCC 
TATAATAGTC AAGAAACCTG TGTGATGACT CCTTCCCCAT GTTCTACACC ACCAGTCAAG 
TCACTGGAGG AGGATCCATG GCCAAGGGTG AATTCTAAGG ACCATATACC TGCCCTGGTT 
CGTAGCAATG CCTTCTCAGA GAATTTTTTA GAGGTTTCAG CTGAGATAGC TCGAGAGAAT 
GTCCAAGGTG TAGTCATACC CTCAAAAGAT CCAGAACCAC TTGAAGAAAA TTGCGCTAAA 
GCCCTGACTT TAAGGATACA TGATTCTTTG AATAATAGCC TTCCAATTGG CCTTGTGCCT 
ACTAATTCAA CAAACACTGT CATGGACCAA AAAAATTTGA AGATGTCAAC TCCTGGCCAA 
ATGAAAGCCC AAGAAATTGA AAGAACCCCT CCAACAAACT TTAAAAGGAC ATTAGAAGAG 
TCCAATTCTG GCCCCCTGAT GAAGAAGCAT AGACGAAATG GCTTAAGTCG AAGTAGTGGT 
GCTCAGCCTG CAAGTCTCCC CACAACCTCA CAGCGAAAGA ACTCTGTTAA ACTCACCATG 
CGACGCAGAC TTAGGGGCCA GAAGAAAATT GGAAATCCTT TACTTCATCA ACACAGGAAA 
ACTGTTTGTG TTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003611.1
CDS	233..2050
Translation	MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPTAEEKPSPRRSLSQPLPPRPSPAALPGGEVSGKGPAVAAREARDPLPDAGASPAPSRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHKPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLEEDPWPRVNSKDHIPALVRSNAFSENFLEVSAEIARENVQGVVIPSKDPEPLEENCAKALTLRIHDSLNNSLPIGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRKTVCVC

Target ORF information:

RefSeq Version	NM_003620.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1D (PPM1D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003620.3

ORF Insert Sequence:

ATGGCGGGGC TGTACTCGCT GGGAGTGAGC GTCTTCTCCG ACCAGGGCGG GAGGAAGTAC 
ATGGAGGACG TTACTCAAAT CGTTGTGGAG CCCGAACCGA CGGCTGAAGA AAAGCCCTCG 
CCGCGGCGGT CGCTGTCTCA GCCGTTGCCT CCGCGGCCGT CGCCGGCCGC CCTTCCCGGC 
GGCGAAGTCT CGGGGAAAGG CCCAGCGGTG GCAGCCCGAG AGGCTCGCGA CCCTCTCCCG 
GACGCCGGGG CCTCGCCGGC ACCTAGCCGC TGCTGCCGCC GCCGTTCCTC CGTGGCCTTT 
TTCGCCGTGT GCGACGGGCA CGGCGGGCGG GAGGCGGCAC AGTTTGCCCG GGAGCACTTG 
TGGGGTTTCA TCAAGAAGCA GAAGGGTTTC ACCTCGTCCG AGCCGGCTAA GGTTTGCGCT 
GCCATCCGCA AAGGCTTTCT CGCTTGTCAC CTTGCCATGT GGAAGAAACT GGCGGAATGG 
CCAAAGACTA TGACGGGTCT TCCTAGCACA TCAGGGACAA CTGCCAGTGT GGTCATCATT 
CGGGGCATGA AGATGTATGT AGCTCACGTA GGTGACTCAG GGGTGGTTCT TGGAATTCAG 
GATGACCCGA AGGATGACTT TGTCAGAGCT GTGGAGGTGA CACAGGACCA TAAGCCAGAA 
CTTCCCAAGG AAAGAGAACG AATCGAAGGA CTTGGTGGGA GTGTAATGAA CAAGTCTGGG 
GTGAATCGTG TAGTTTGGAA ACGACCTCGA CTCACTCACA ATGGACCTGT TAGAAGGAGC 
ACAGTTATTG ACCAGATTCC TTTTCTGGCA GTAGCAAGAG CACTTGGTGA TTTGTGGAGC 
TATGATTTCT TCAGTGGTGA ATTTGTGGTG TCACCTGAAC CAGACACAAG TGTCCACACT 
CTTGACCCTC AGAAGCACAA GTATATTATA TTGGGGAGTG ATGGACTTTG GAATATGATT 
CCACCACAAG ATGCCATCTC AATGTGCCAG GACCAAGAGG AGAAAAAATA CCTGATGGGT 
GAGCATGGAC AATCTTGTGC CAAAATGCTT GTGAATCGAG CATTGGGCCG CTGGAGGCAG 
CGTATGCTCC GAGCAGATAA CACTAGTGCC ATAGTAATCT GCATCTCTCC AGAAGTGGAC 
AATCAGGGAA ACTTTACCAA TGAAGATGAG TTATACCTGA ACCTGACTGA CAGCCCTTCC 
TATAATAGTC AAGAAACCTG TGTGATGACT CCTTCCCCAT GTTCTACACC ACCAGTCAAG 
TCACTGGAGG AGGATCCATG GCCAAGGGTG AATTCTAAGG ACCATATACC TGCCCTGGTT 
CGTAGCAATG CCTTCTCAGA GAATTTTTTA GAGGTTTCAG CTGAGATAGC TCGAGAGAAT 
GTCCAAGGTG TAGTCATACC CTCAAAAGAT CCAGAACCAC TTGAAGAAAA TTGCGCTAAA 
GCCCTGACTT TAAGGATACA TGATTCTTTG AATAATAGCC TTCCAATTGG CCTTGTGCCT 
ACTAATTCAA CAAACACTGT CATGGACCAA AAAAATTTGA AGATGTCAAC TCCTGGCCAA 
ATGAAAGCCC AAGAAATTGA AAGAACCCCT CCAACAAACT TTAAAAGGAC ATTAGAAGAG 
TCCAATTCTG GCCCCCTGAT GAAGAAGCAT AGACGAAATG GCTTAAGTCG AAGTAGTGGT 
GCTCAGCCTG CAAGTCTCCC CACAACCTCA CAGCGAAAGA ACTCTGTTAA ACTCACCATG 
CGACGCAGAC TTAGGGGCCA GAAGAAAATT GGAAATCCTT TACTTCATCA ACACAGGAAA 
ACTGTTTGTG TTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Detectible mosaic truncating PPM1D mutations, age and breast cancer risk.
Journal of human genetics64(6)545-550(2019 Jun)
Machiela MJ,Myers TA,Lyons CJ,Koster R,Figg WD Jr,Colli LM,Jessop L,Ahearn TU,Freedman ND,García-Closas M,Chanock SJ

The Regulation of Trophoblastic p53 Homeostasis by the p38-Wip1 Feedback Loop is Disturbed in Placentas from Pregnancies Complicated by Preeclampsia.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology52(2)315-335(2019)
Tan B,Tong C,Yuan Y,Xu P,Wen L,Zhang C,Zheng Y,Lin L,Zhu F,Gui S,Wang L,Gao R,Li J,Qi H,Baker PN

Absence of EIF1AX, PPM1D, and CHEK2 mutations reported in Thyroid Cancer Genome Atlas (TCGA) in a large series of thyroid cancer.
Endocrine63(1)94-100(2019 01)
Alzahrani AS,Murugan AK,Qasem E,Alswailem MM,AlGhamdi B,Moria Y,Al-Hindi H

Wip1 knockout inhibits neurogenesis by affecting the Wnt/β-catenin signaling pathway in focal cerebral ischemia in mice.
Experimental neurology30944-53(2018 11)
Qiu CW,Liu ZY,Hou K,Liu SY,Hu YX,Zhang L,Zhang FL,Lv KY,Kang Q,Hu WY,Ma N,Jiao Y,Bai WJ,Xiao ZC

Blood132(11)1095-1105(2018 09)
Kahn JD,Miller PG,Silver AJ,Sellar RS,Bhatt S,Gibson C,McConkey M,Adams D,Mar B,Mertins P,Fereshetian S,Krug K,Zhu H,Letai A,Carr SA,Doench J,Jaiswal S,Ebert BL

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

PPM1D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM