| Gene Symbol | VPS25 |
| Entrez Gene ID | 84313 |
| Full Name | vacuolar protein sorting 25 homolog |
| Synonyms | DERP9,EAP20,FAP20 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]. |
| Disorder MIM: |
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VPS25 cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following VPS25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu87567 Clone ID Related Accession (Same CDS sequence) XM_017025224.1 Accession Version XM_017025224.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 555bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product vacuolar protein-sorting-associated protein 25 isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541ATGGCGATGA GTTTCGAGTG GCCGTGGCAG TATCGCTTCC CACCCTTCTT TACGTTACAA
CCGAATGTGG ACACTCGGCA GAAGCAGCTG GCCGCCTGGT GCTCGCTGGT CCTGTCCTTC
TGCCGCCTGC ACAAACAGTC CAGCATGACG GTGATGGAAG CTCAGGAGAG CCCGCTCTTC
AACAACGTCA AGCTACAGCG AAAGCTTCCT GTGGAGTCGA TCCAGATTGT ATTAGAGGAA
CTGAGGAAGA AAGGGAACCT CGAGTGGTTG GATAAGAGCA AGTCCAGCTT CCTGATCATG
TGGCGGAGGC CAGAAGAATG GGGGAAACTC ATCTATCAGT GGGTTTCCAG GAGTGGCCAG
AACAACTCCG TCTTTACCCT GTATGAACTG ACTAATGGGG AAGACACAGA GGATGAGGGT
AATGCCTTTC CCTTCCCACT CATAGTTAAT TTTTTGTTTT GTTTTGTTTT GTTTTTCCGA
TATGGAGTCT TGCTCTGTCA CCCAGGCTGG AGTGCAATGT TGCAATCTCG GCTCACTGCA
ACCTCCGCCT CCTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016880713.1 CDS 7..561 Translation 
Target ORF information:
RefSeq Version XM_017025224.1 Organism Homo sapiens(human) Definition Homo sapiens vacuolar protein sorting 25 homolog (VPS25), transcript variant X1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
XM_017025224.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541ATGGCGATGA GTTTCGAGTG GCCGTGGCAG TATCGCTTCC CACCCTTCTT TACGTTACAA
CCGAATGTGG ACACTCGGCA GAAGCAGCTG GCCGCCTGGT GCTCGCTGGT CCTGTCCTTC
TGCCGCCTGC ACAAACAGTC CAGCATGACG GTGATGGAAG CTCAGGAGAG CCCGCTCTTC
AACAACGTCA AGCTACAGCG AAAGCTTCCT GTGGAGTCGA TCCAGATTGT ATTAGAGGAA
CTGAGGAAGA AAGGGAACCT CGAGTGGTTG GATAAGAGCA AGTCCAGCTT CCTGATCATG
TGGCGGAGGC CAGAAGAATG GGGGAAACTC ATCTATCAGT GGGTTTCCAG GAGTGGCCAG
AACAACTCCG TCTTTACCCT GTATGAACTG ACTAATGGGG AAGACACAGA GGATGAGGGT
AATGCCTTTC CCTTCCCACT CATAGTTAAT TTTTTGTTTT GTTTTGTTTT GTTTTTCCGA
TATGGAGTCT TGCTCTGTCA CCCAGGCTGG AGTGCAATGT TGCAATCTCG GCTCACTGCA
ACCTCCGCCT CCTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu10037 Clone ID Related Accession (Same CDS sequence) NM_032353.3 , NM_032353.4 Accession Version NM_032353.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 531bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1504886400000 Organism Homo sapiens(human) Product vacuolar protein-sorting-associated protein 25 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006282.2 and BU630023.1. On Jul 30, 2013 this sequence version replaced NM_032353.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006282.2, SRR1163655.708590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481ATGGCGATGA GTTTCGAGTG GCCGTGGCAG TATCGCTTCC CACCCTTCTT TACGTTACAA
CCGAATGTGG ACACTCGGCA GAAGCAGCTG GCCGCCTGGT GCTCGCTGGT CCTGTCCTTC
TGCCGCCTGC ACAAACAGTC CAGCATGACG GTGATGGAAG CTCAGGAGAG CCCGCTCTTC
AACAACGTCA AGCTACAGCG AAAGCTTCCT GTGGAGTCGA TCCAGATTGT ATTAGAGGAA
CTGAGGAAGA AAGGGAACCT CGAGTGGTTG GATAAGAGCA AGTCCAGCTT CCTGATCATG
TGGCGGAGGC CAGAAGAATG GGGGAAACTC ATCTATCAGT GGGTTTCCAG GAGTGGCCAG
AACAACTCCG TCTTTACCCT GTATGAACTG ACTAATGGGG AAGACACAGA GGATGAGGAG
TTCCACGGGC TGGATGAAGC CACTCTACTG CGGGCTCTGC AGGCCCTACA GCAGGAGCAC
AAGGCCGAGA TCATCACTGT CAGCGATGGC CGAGGCGTCA AGTTCTTCTA GThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_115729.1 CDS 41..571 Translation
Target ORF information:
RefSeq Version NM_032353.3 Organism Homo sapiens(human) Definition Homo sapiens vacuolar protein sorting 25 homolog (VPS25), mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_032353.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481ATGGCGATGA GTTTCGAGTG GCCGTGGCAG TATCGCTTCC CACCCTTCTT TACGTTACAA
CCGAATGTGG ACACTCGGCA GAAGCAGCTG GCCGCCTGGT GCTCGCTGGT CCTGTCCTTC
TGCCGCCTGC ACAAACAGTC CAGCATGACG GTGATGGAAG CTCAGGAGAG CCCGCTCTTC
AACAACGTCA AGCTACAGCG AAAGCTTCCT GTGGAGTCGA TCCAGATTGT ATTAGAGGAA
CTGAGGAAGA AAGGGAACCT CGAGTGGTTG GATAAGAGCA AGTCCAGCTT CCTGATCATG
TGGCGGAGGC CAGAAGAATG GGGGAAACTC ATCTATCAGT GGGTTTCCAG GAGTGGCCAG
AACAACTCCG TCTTTACCCT GTATGAACTG ACTAATGGGG AAGACACAGA GGATGAGGAG
TTCCACGGGC TGGATGAAGC CACTCTACTG CGGGCTCTGC AGGCCCTACA GCAGGAGCAC
AAGGCCGAGA TCATCACTGT CAGCGATGGC CGAGGCGTCA AGTTCTTCTA GThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu10037 Clone ID Related Accession (Same CDS sequence) NM_032353.3 , NM_032353.4 Accession Version NM_032353.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 531bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1570550400000 Organism Homo sapiens(human) Product vacuolar protein-sorting-associated protein 25 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006282.2. On Nov 23, 2018 this sequence version replaced NM_032353.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006282.2, SRR5189658.41531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253794.7/ ENSP00000253794.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.
1
61
121
181
241
301
361
421
481ATGGCGATGA GTTTCGAGTG GCCGTGGCAG TATCGCTTCC CACCCTTCTT TACGTTACAA
CCGAATGTGG ACACTCGGCA GAAGCAGCTG GCCGCCTGGT GCTCGCTGGT CCTGTCCTTC
TGCCGCCTGC ACAAACAGTC CAGCATGACG GTGATGGAAG CTCAGGAGAG CCCGCTCTTC
AACAACGTCA AGCTACAGCG AAAGCTTCCT GTGGAGTCGA TCCAGATTGT ATTAGAGGAA
CTGAGGAAGA AAGGGAACCT CGAGTGGTTG GATAAGAGCA AGTCCAGCTT CCTGATCATG
TGGCGGAGGC CAGAAGAATG GGGGAAACTC ATCTATCAGT GGGTTTCCAG GAGTGGCCAG
AACAACTCCG TCTTTACCCT GTATGAACTG ACTAATGGGG AAGACACAGA GGATGAGGAG
TTCCACGGGC TGGATGAAGC CACTCTACTG CGGGCTCTGC AGGCCCTACA GCAGGAGCAC
AAGGCCGAGA TCATCACTGT CAGCGATGGC CGAGGCGTCA AGTTCTTCTA GThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_115729.1 CDS 28..558 Translation
Target ORF information:
RefSeq Version NM_032353.4 Organism Homo sapiens(human) Definition Homo sapiens vacuolar protein sorting 25 homolog (VPS25), mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_032353.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481ATGGCGATGA GTTTCGAGTG GCCGTGGCAG TATCGCTTCC CACCCTTCTT TACGTTACAA
CCGAATGTGG ACACTCGGCA GAAGCAGCTG GCCGCCTGGT GCTCGCTGGT CCTGTCCTTC
TGCCGCCTGC ACAAACAGTC CAGCATGACG GTGATGGAAG CTCAGGAGAG CCCGCTCTTC
AACAACGTCA AGCTACAGCG AAAGCTTCCT GTGGAGTCGA TCCAGATTGT ATTAGAGGAA
CTGAGGAAGA AAGGGAACCT CGAGTGGTTG GATAAGAGCA AGTCCAGCTT CCTGATCATG
TGGCGGAGGC CAGAAGAATG GGGGAAACTC ATCTATCAGT GGGTTTCCAG GAGTGGCCAG
AACAACTCCG TCTTTACCCT GTATGAACTG ACTAATGGGG AAGACACAGA GGATGAGGAG
TTCCACGGGC TGGATGAAGC CACTCTACTG CGGGCTCTGC AGGCCCTACA GCAGGAGCAC
AAGGCCGAGA TCATCACTGT CAGCGATGGC CGAGGCGTCA AGTTCTTCTA GThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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PubMed
Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW
Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M
Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,
Membrane budding and scission by the ESCRT machinery: it's all in the neck.
Nature reviews. Molecular cell biology11(8)556-66(2010 Aug)
Hurley JH,Hanson PI
Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,
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