NLN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLN
Entrez Gene ID	57486
Full Name	neurolysin
Synonyms	AGTBP,EP24.16,MEP,MOP
General protein information	Preferred Names neurolysin Names neurolysin, mitochondrial angiotensin binding protein endopeptidase 24.16 microsomal endopeptidase mitochondrial oligopeptidase M neurolysin (metallopeptidase M3 family) neurotensin endopeptidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q12.3
Summary	This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.[provided by RefSeq, Jun 2010].
Disorder MIM:	611530

mRNA and Protein(s)

mRNA	Protein	Name
NM_020726.4	NP_065777.1	neurolysin, mitochondrial
XM_005248559.3	XP_005248616.1	neurolysin, mitochondrial isoform X1
XM_006714661.2	XP_006714724.1	neurolysin, mitochondrial isoform X2
NM_020726.5	NP_065777.1	neurolysin, mitochondrial
XM_005248559.2	XP_005248616.1	neurolysin, mitochondrial isoform X1
XM_017009673.1	XP_016865162.1	neurolysin, mitochondrial isoform X3

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04858	XP_003712337.1
Pan troglodytes (chimpanzee)	NLN	XP_001163138.1
Rattus norvegicus (Norway rat)	Nln	NP_446422.2
Mus musculus (house mouse)	Nln	NP_083723.1
Kluyveromyces lactis	KLLA0C00572g	XP_452219.1
Neurospora crassa	NCU06618	XP_962360.1
Macaca mulatta (Rhesus monkey)	LOC696884	XP_001087239.1
Homo sapiens (human)	NLN	NP_065777.1
Canis lupus familiaris (dog)	NLN	XP_005617505.1
Bos taurus (cattle)	NLN	NP_001029161.2
Saccharomyces cerevisiae (baker's yeast)	PRD1	NP_009874.1
Xenopus tropicalis (tropical clawed frog)	nln	XP_002934236.2
Gallus gallus (chicken)	NLN	XP_424755.2

Gene Ontology
Bibliography

Related articles in PubMed

Mechanism of Peptide Binding and Cleavage by the Human Mitochondrial Peptidase Neurolysin.
Teixeira PF, Masuyer G, Pinho CM, Branca RMM, Kmiec B, Wallin C, Wärmländer SKTS, Berntsson RP, Ankarcrona M, Gräslund A, Lehtiö J, Stenmark P, Glaser E
Journal of molecular biology430(3)348-362(2018 02)

Genome-wide association study for refractive astigmatism reveals genetic co-determination with spherical equivalent refractive error: the CREAM consortium.
Li Q, Wojciechowski R, Simpson CL, Hysi PG, Verhoeven VJ, Ikram MK, Höhn R, Vitart V, Hewitt AW, Oexle K, Mäkelä KM, MacGregor S, Pirastu M, Fan Q, Cheng CY, St Pourcain B, McMahon G, Kemp JP, Northstone K, Rahi JS, Cumberland PM, Martin NG, Sanfilippo PG, Lu Y, Wang YX, Hayward C, Polašek O, Campbell H, Bencic G, Wright AF, Wedenoja J, Zeller T, Schillert A, Mirshahi A, Lackner K, Yip SP, Yap MK, Ried JS, Gieger C, Murgia F, Wilson JF, Fleck B, Yazar S, Vingerling JR, Hofman A, Uitterlinden A, Rivadeneira F, Amin N, Karssen L, Oostra BA, Zhou X, Teo YY, Tai ES, Vithana E, Barathi V, Zheng Y, Siantar RG, Neelam K, Shin Y, Lam J, Yonova-Doing E, Venturini C, Hosseini SM, Wong HS, Lehtimäki T, Kähönen M, Raitakari O, Timpson NJ, Evans DM, Khor CC, Aung T, Young TL, Mitchell P, Klein B, van Duijn CM, Meitinger T, Jonas JB, Baird PN, Mackey DA, Wong TY, Saw SM, Pärssinen O, Stambolian D, Hammond CJ, Klaver CC, Williams C, Paterson AD, Bailey-Wilson JE, Guggenheim JA,
Human genetics134(2)131-46(2015 Feb)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Swapping the substrate specificities of the neuropeptidases neurolysin and thimet oligopeptidase.
Lim EJ, Sampath S, Coll-Rodriguez J, Schmidt J, Ray K, Rodgers DW
The Journal of biological chemistry282(13)9722-32(2007 Mar)

Endopeptidases 3.4.24.15 and 24.16 in endothelial cells: potential role in vasoactive peptide metabolism.
Norman MU, Reeve SB, Dive V, Smith AI, Lew RA
American journal of physiology. Heart and circulatory physiology284(6)H1978-84(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu39650	XM_005248559.3 Latest version!	Homo sapiens neurolysin (NLN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu39651	XM_006714661.2 Latest version!	Homo sapiens neurolysin (NLN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu95969	XM_017009673.1 Latest version!	Homo sapiens neurolysin (NLN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu10904	NM_020726.5 Latest version!	Homo sapiens neurolysin (NLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$496.30 $709.00
View Old Accession Versions >>
OHu10904	NM_020726.4	Homo sapiens neurolysin (NLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$496.30 $709.00
OHu39650	XM_005248559.2	Homo sapiens neurolysin (NLN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLN ( XM_005248559.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248616.1 Homo sapiens neurolysin (NLN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39650
Clone ID Related Accession (Same CDS sequence)	XM_005248559.3 , XM_005248559.2
Accession Version	XM_005248559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	neurolysin, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGACTGATT TTGCACGATT TAGCGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCGCAAATGC TTGAAAATTG GGTGTGGGAC 
GTCGATTCCC TCCGAAGATT GTCAAAACAT TATAAAGATG GAAGCCCTAT TGCAGACGAT 
CTGCTTGAAA AACTTGTTGC TTCTAGGCTG GTCAACACAG GTCTTCTGAC CCTGCGCCAG 
ATTGTTTTGA GCAAAGTTGA TCAGTCTCTT CATACCAACA CATCGCTGGA TGCTGCAAGT 
GAATATGCCA AATACTGCTC AGAAATATTA GGAGTTGCAG CTACTCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGGGGA TACGATGGCC AATATTATGG ATATCTTTGG 
AGTGAAGTAT TTTCCATGGA TATGTTTTAC AGCTGTTTTA AAAAAGAAGG GATAATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAAC CTGGGGGATC TCTGGACGGC 
ATGGACATGC TCCACAATTT CTTGAAACGT GAGCCAAACC AAAAAGCGTT CCTAATGAGT 
AGAGGCCTGC ATGCTCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248616.1
CDS	170..2230
Translation	MIARCLLAVRSLRRVGGSRILLRMTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDETDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Target ORF information:

RefSeq Version	XM_005248559.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neurolysin (NLN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248559.3

ORF Insert Sequence:

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGACTGATT TTGCACGATT TAGCGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCGCAAATGC TTGAAAATTG GGTGTGGGAC 
GTCGATTCCC TCCGAAGATT GTCAAAACAT TATAAAGATG GAAGCCCTAT TGCAGACGAT 
CTGCTTGAAA AACTTGTTGC TTCTAGGCTG GTCAACACAG GTCTTCTGAC CCTGCGCCAG 
ATTGTTTTGA GCAAAGTTGA TCAGTCTCTT CATACCAACA CATCGCTGGA TGCTGCAAGT 
GAATATGCCA AATACTGCTC AGAAATATTA GGAGTTGCAG CTACTCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGGGGA TACGATGGCC AATATTATGG ATATCTTTGG 
AGTGAAGTAT TTTCCATGGA TATGTTTTAC AGCTGTTTTA AAAAAGAAGG GATAATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAAC CTGGGGGATC TCTGGACGGC 
ATGGACATGC TCCACAATTT CTTGAAACGT GAGCCAAACC AAAAAGCGTT CCTAATGAGT 
AGAGGCCTGC ATGCTCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLN ( XM_005248559.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248616.1 Homo sapiens neurolysin (NLN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39650
Clone ID Related Accession (Same CDS sequence)	XM_005248559.3 , XM_005248559.2
Accession Version	XM_005248559.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	neurolysin, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGACTGATT TTGCACGATT TAGCGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCGCAAATGC TTGAAAATTG GGTGTGGGAC 
GTCGATTCCC TCCGAAGATT GTCAAAACAT TATAAAGATG GAAGCCCTAT TGCAGACGAT 
CTGCTTGAAA AACTTGTTGC TTCTAGGCTG GTCAACACAG GTCTTCTGAC CCTGCGCCAG 
ATTGTTTTGA GCAAAGTTGA TCAGTCTCTT CATACCAACA CATCGCTGGA TGCTGCAAGT 
GAATATGCCA AATACTGCTC AGAAATATTA GGAGTTGCAG CTACTCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGGGGA TACGATGGCC AATATTATGG ATATCTTTGG 
AGTGAAGTAT TTTCCATGGA TATGTTTTAC AGCTGTTTTA AAAAAGAAGG GATAATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAAC CTGGGGGATC TCTGGACGGC 
ATGGACATGC TCCACAATTT CTTGAAACGT GAGCCAAACC AAAAAGCGTT CCTAATGAGT 
AGAGGCCTGC ATGCTCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248616.1
CDS	170..2230
Translation	MIARCLLAVRSLRRVGGSRILLRMTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDETDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Target ORF information:

RefSeq Version	XM_005248559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurolysin (NLN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248559.2

ORF Insert Sequence:

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGACTGATT TTGCACGATT TAGCGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCGCAAATGC TTGAAAATTG GGTGTGGGAC 
GTCGATTCCC TCCGAAGATT GTCAAAACAT TATAAAGATG GAAGCCCTAT TGCAGACGAT 
CTGCTTGAAA AACTTGTTGC TTCTAGGCTG GTCAACACAG GTCTTCTGAC CCTGCGCCAG 
ATTGTTTTGA GCAAAGTTGA TCAGTCTCTT CATACCAACA CATCGCTGGA TGCTGCAAGT 
GAATATGCCA AATACTGCTC AGAAATATTA GGAGTTGCAG CTACTCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGGGGA TACGATGGCC AATATTATGG ATATCTTTGG 
AGTGAAGTAT TTTCCATGGA TATGTTTTAC AGCTGTTTTA AAAAAGAAGG GATAATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAAC CTGGGGGATC TCTGGACGGC 
ATGGACATGC TCCACAATTT CTTGAAACGT GAGCCAAACC AAAAAGCGTT CCTAATGAGT 
AGAGGCCTGC ATGCTCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLN ( XM_006714661.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714724.1 Homo sapiens neurolysin (NLN), transcript variant X2, mRNA.

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CloneID	OHu39651
Clone ID Related Accession (Same CDS sequence)	XM_006714661.2
Accession Version	XM_006714661.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	neurolysin, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTAG GAAGAGAAGT GATGTCTCCT CTTCAGGCAA TGTCTTCCTA TACTGTGGCT 
GGCAGAAATG TTTTAAGATG GGATCTTTCA CCAGAGCAAA TTAAAACAAG AACTGAGGAG 
CTCATTGTGC AGACCAAACA GGTGTACGAT GCTGTTGGAA TGCTCGGTAT TGAGGAAGTA 
ACTTACGAGA ACTGTCTGCA GGCACTGGCA GATGTAGAAG TAAAGTATAT AGTGGAAAGG 
ACCATGCTAG ACTTTCCCCA GCATGTATCC TCTGACAAAG AAGTACGAGC AGCAAGTACA 
GAAGCAGACA AAAGACTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGG AGATATATTT 
GAGAGAATTG TTCATTTACA GGAAACCTGT GATCTGGGGA AGATAAAACC TGAGGCCAGA 
CGATACTTGG AAAAGTCAAT TAAAATGGGG AAAAGAAATG GGCTCCATCT TCCTGAACAA 
GTACAGAATG AAATCAAATC AATGAAGAAA AGAATGAGTG AGCTATGTAT TGATTTTAAC 
AAAAACCTCA ATGAGGATGA TACCTTCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGATT TCATTGACAG TTTAGAAAAG ACAGATGATG ACAAGTATAA AATTACCTTA 
AAATATCCAC ACTATTTCCC TGTCATGAAG AAATGTTGTA TCCCTGAAAC CAGAAGAAGG 
ATGGAAATGG CTTTTAATAC AAGGTGCAAA GAGGAAAACA CCATAATTTT GCAGCAGCTA 
CTCCCACTGC GAACCAAGGT GGCCAAACTA CTCGGTTATA GCACACATGC TGACTTCGTC 
CTTGAAATGA ACACTGCAAA GAGCACAAGC CGCGTAACAG CCTTTCTAGA TGATTTAAGC 
CAGAAGTTAA AACCCTTGGG TGAAGCAGAA CGAGAGTTTA TTTTGAATTT GAAGAAAAAG 
GAATGCAAAG ACAGGGGTTT TGAATATGAT GGGAAAATCA ATGCCTGGGA TCTATATTAC 
TACATGACTC AGACAGAGGA ACTCAAGTAT TCCATAGACC AAGAGTTCCT CAAGGAATAC 
TTCCCAATTG AGGTGGTCAC TGAAGGCTTG CTGAACACCT ACCAGGAGTT GTTGGGACTT 
TCATTTGAAC AAATGACAGA TGCTCATGTT TGGAACAAGA GTGTTACACT TTATACTGTG 
AAGGATAAAG CTACAGGAGA AGTATTGGGA CAGTTCTATT TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ATCATGCGGC CTGCTTCGGT CTCCAGCCTG GCTGCCTTCT GCCTGATGGA 
AGCCGGATGA TGGCAGTGGC TGCCCTCGTG GTGAACTTCT CACAGCCAGT GGCAGGTCGT 
CCCTCTCTCC TGAGACACGA CGAGGTGAGG ACTTACTTTC ATGAGTTTGG TCACGTGATG 
CATCAGATTT GTGCACAGAC TGATTTTGCA CGATTTAGCG GAACAAATGT GGAAACTGAC 
TTTGTAGAGG TGCCATCGCA AATGCTTGAA AATTGGGTGT GGGACGTCGA TTCCCTCCGA 
AGATTGTCAA AACATTATAA AGATGGAAGC CCTATTGCAG ACGATCTGCT TGAAAAACTT 
GTTGCTTCTA GGCTGGTCAA CACAGGTCTT CTGACCCTGC GCCAGATTGT TTTGAGCAAA 
GTTGATCAGT CTCTTCATAC CAACACATCG CTGGATGCTG CAAGTGAATA TGCCAAATAC 
TGCTCAGAAA TATTAGGAGT TGCAGCTACT CCAGGCACAA ATATGCCAGC TACCTTTGGA 
CATTTGGCAG GGGGATACGA TGGCCAATAT TATGGATATC TTTGGAGTGA AGTATTTTCC 
ATGGATATGT TTTACAGCTG TTTTAAAAAA GAAGGGATAA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACCTAATCCT GAAACCTGGG GGATCTCTGG ACGGCATGGA CATGCTCCAC 
AATTTCTTGA AACGTGAGCC AAACCAAAAA GCGTTCCTAA TGAGTAGAGG CCTGCATGCT 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714724.1
CDS	303..2348
Translation	MTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Target ORF information:

RefSeq Version	XM_006714661.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurolysin (NLN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714661.2

ORF Insert Sequence:

ATGACGTTAG GAAGAGAAGT GATGTCTCCT CTTCAGGCAA TGTCTTCCTA TACTGTGGCT 
GGCAGAAATG TTTTAAGATG GGATCTTTCA CCAGAGCAAA TTAAAACAAG AACTGAGGAG 
CTCATTGTGC AGACCAAACA GGTGTACGAT GCTGTTGGAA TGCTCGGTAT TGAGGAAGTA 
ACTTACGAGA ACTGTCTGCA GGCACTGGCA GATGTAGAAG TAAAGTATAT AGTGGAAAGG 
ACCATGCTAG ACTTTCCCCA GCATGTATCC TCTGACAAAG AAGTACGAGC AGCAAGTACA 
GAAGCAGACA AAAGACTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGG AGATATATTT 
GAGAGAATTG TTCATTTACA GGAAACCTGT GATCTGGGGA AGATAAAACC TGAGGCCAGA 
CGATACTTGG AAAAGTCAAT TAAAATGGGG AAAAGAAATG GGCTCCATCT TCCTGAACAA 
GTACAGAATG AAATCAAATC AATGAAGAAA AGAATGAGTG AGCTATGTAT TGATTTTAAC 
AAAAACCTCA ATGAGGATGA TACCTTCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGATT TCATTGACAG TTTAGAAAAG ACAGATGATG ACAAGTATAA AATTACCTTA 
AAATATCCAC ACTATTTCCC TGTCATGAAG AAATGTTGTA TCCCTGAAAC CAGAAGAAGG 
ATGGAAATGG CTTTTAATAC AAGGTGCAAA GAGGAAAACA CCATAATTTT GCAGCAGCTA 
CTCCCACTGC GAACCAAGGT GGCCAAACTA CTCGGTTATA GCACACATGC TGACTTCGTC 
CTTGAAATGA ACACTGCAAA GAGCACAAGC CGCGTAACAG CCTTTCTAGA TGATTTAAGC 
CAGAAGTTAA AACCCTTGGG TGAAGCAGAA CGAGAGTTTA TTTTGAATTT GAAGAAAAAG 
GAATGCAAAG ACAGGGGTTT TGAATATGAT GGGAAAATCA ATGCCTGGGA TCTATATTAC 
TACATGACTC AGACAGAGGA ACTCAAGTAT TCCATAGACC AAGAGTTCCT CAAGGAATAC 
TTCCCAATTG AGGTGGTCAC TGAAGGCTTG CTGAACACCT ACCAGGAGTT GTTGGGACTT 
TCATTTGAAC AAATGACAGA TGCTCATGTT TGGAACAAGA GTGTTACACT TTATACTGTG 
AAGGATAAAG CTACAGGAGA AGTATTGGGA CAGTTCTATT TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ATCATGCGGC CTGCTTCGGT CTCCAGCCTG GCTGCCTTCT GCCTGATGGA 
AGCCGGATGA TGGCAGTGGC TGCCCTCGTG GTGAACTTCT CACAGCCAGT GGCAGGTCGT 
CCCTCTCTCC TGAGACACGA CGAGGTGAGG ACTTACTTTC ATGAGTTTGG TCACGTGATG 
CATCAGATTT GTGCACAGAC TGATTTTGCA CGATTTAGCG GAACAAATGT GGAAACTGAC 
TTTGTAGAGG TGCCATCGCA AATGCTTGAA AATTGGGTGT GGGACGTCGA TTCCCTCCGA 
AGATTGTCAA AACATTATAA AGATGGAAGC CCTATTGCAG ACGATCTGCT TGAAAAACTT 
GTTGCTTCTA GGCTGGTCAA CACAGGTCTT CTGACCCTGC GCCAGATTGT TTTGAGCAAA 
GTTGATCAGT CTCTTCATAC CAACACATCG CTGGATGCTG CAAGTGAATA TGCCAAATAC 
TGCTCAGAAA TATTAGGAGT TGCAGCTACT CCAGGCACAA ATATGCCAGC TACCTTTGGA 
CATTTGGCAG GGGGATACGA TGGCCAATAT TATGGATATC TTTGGAGTGA AGTATTTTCC 
ATGGATATGT TTTACAGCTG TTTTAAAAAA GAAGGGATAA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACCTAATCCT GAAACCTGGG GGATCTCTGG ACGGCATGGA CATGCTCCAC 
AATTTCTTGA AACGTGAGCC AAACCAAAAA GCGTTCCTAA TGAGTAGAGG CCTGCATGCT 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLN ( XM_017009673.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865162.1 Homo sapiens neurolysin (NLN), transcript variant X3, mRNA.

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CloneID	OHu95969
Clone ID Related Accession (Same CDS sequence)	XM_017009673.1
Accession Version	XM_017009673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	neurolysin, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTAG GAAGAGAAGT GATGTCTCCT CTTCAGGCAA TGTCTTCCTA TACTGTGGCT 
GGCAGAAATG TTTTAAGATG GGATCTTTCA CCAGAGCAAA TTAAAACAAG AACTGAGGAG 
CTCATTGTGC AGACCAAACA GGTGTACGAT GCTGTTGGAA TGCTCGGTAT TGAGGAAGTA 
ACTTACGAGA ACTGTCTGCA GGCACTGGCA GATGTAGAAG TAAAGTATAT AGTGGAAAGG 
ACCATGCTAG ACTTTCCCCA GCATGTATCC TCTGACAAAG AAGTACGAGC AGCAAGTACA 
GAAGCAGACA AAAGACTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGG AGATATATTT 
GAGAGAATTG TTCATTTACA GGAAACCTGT GATCTGGGGA AGATAAAACC TGAGGCCAGA 
CGATACTTGG AAAAGTCAAT TAAAATGGGG AAAAGAAATG GGCTCCATCT TCCTGAACAA 
GTACAGAATG AAATCAAATC AATGAAGAAA AGAATGAGTG AGCTATGTAT TGATTTTAAC 
AAAAACCTCA ATGAGGATGA TACCTTCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGATT TCATTGACAG TTTAGAAAAG ACAGATGATG ACAAGTATAA AATTACCTTA 
AAATATCCAC ACTATTTCCC TGTCATGAAG AAATGTTGTA TCCCTGAAAC CAGAAGAAGG 
ATGGAAATGG CTTTTAATAC AAGGTGCAAA GAGGAAAACA CCATAATTTT GCAGCAGCTA 
CTCCCACTGC GAACCAAGGT GGCCAAACTA CTCGGTTATA GCACACATGC TGACTTCGTC 
CTTGAAATGA ACACTGCAAA GAGCACAAGC CGCGTAACAG CCTTTCTAGA TGATTTAAGC 
CAGAAGTTAA AACCCTTGGG TGAAGCAGAA CGAGAGTTTA TTTTGAATTT GAAGAAAAAG 
GAATGCAAAG ACAGGGGTTT TGAATATGAT GGGAAAATCA ATGCCTGGGA TCTATATTAC 
TACATGACTC AGACAGAGGA ACTCAAGTAT TCCATAGACC AAGAGTTCCT CAAGGAATAC 
TTCCCAATTG AGGTGGTCAC TGAAGGCTTG CTGAACACCT ACCAGGAGTT GTTGGGACTT 
TCATTTGAAC AAATGACAGA TGCTCATGTT TGGAACAAGA GTGTTACACT TTATACTGTG 
AAGGATAAAG CTACAGGAGA AGTATTGGGA CAGTTCTATT TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ATCATGCGGC CTGCTTCGGT CTCCAGCCTG GCTGCCTTCT GCCTGATGGA 
AGCCGGATGA TGGCAGTGGC TGCCCTCGTG GTGAACTTCT CACAGCCAGT GGCAGGTCGT 
CCCTCTCTCC TGAGACACGA CGAGACTGAT TTTGCACGAT TTAGCGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCGCAAATG CTTGAAAATT GGGTGTGGGA CGTCGATTCC 
CTCCGAAGAT TGTCAAAACA TTATAAAGAT GGAAGCCCTA TTGCAGACGA TCTGCTTGAA 
AAACTTGTTG CTTCTAGGCT GGTCAACACA GGTCTTCTGA CCCTGCGCCA GATTGTTTTG 
AGCAAAGTTG ATCAGTCTCT TCATACCAAC ACATCGCTGG ATGCTGCAAG TGAATATGCC 
AAATACTGCT CAGAAATATT AGGAGTTGCA GCTACTCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGGGG ATACGATGGC CAATATTATG GATATCTTTG GAGTGAAGTA 
TTTTCCATGG ATATGTTTTA CAGCTGTTTT AAAAAAGAAG GGATAATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAA CCTGGGGGAT CTCTGGACGG CATGGACATG 
CTCCACAATT TCTTGAAACG TGAGCCAAAC CAAAAAGCGT TCCTAATGAG TAGAGGCCTG 
CATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865162.1
CDS	303..2294
Translation	MTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDETDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Target ORF information:

RefSeq Version	XM_017009673.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neurolysin (NLN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009673.1

ORF Insert Sequence:

ATGACGTTAG GAAGAGAAGT GATGTCTCCT CTTCAGGCAA TGTCTTCCTA TACTGTGGCT 
GGCAGAAATG TTTTAAGATG GGATCTTTCA CCAGAGCAAA TTAAAACAAG AACTGAGGAG 
CTCATTGTGC AGACCAAACA GGTGTACGAT GCTGTTGGAA TGCTCGGTAT TGAGGAAGTA 
ACTTACGAGA ACTGTCTGCA GGCACTGGCA GATGTAGAAG TAAAGTATAT AGTGGAAAGG 
ACCATGCTAG ACTTTCCCCA GCATGTATCC TCTGACAAAG AAGTACGAGC AGCAAGTACA 
GAAGCAGACA AAAGACTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGG AGATATATTT 
GAGAGAATTG TTCATTTACA GGAAACCTGT GATCTGGGGA AGATAAAACC TGAGGCCAGA 
CGATACTTGG AAAAGTCAAT TAAAATGGGG AAAAGAAATG GGCTCCATCT TCCTGAACAA 
GTACAGAATG AAATCAAATC AATGAAGAAA AGAATGAGTG AGCTATGTAT TGATTTTAAC 
AAAAACCTCA ATGAGGATGA TACCTTCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGATT TCATTGACAG TTTAGAAAAG ACAGATGATG ACAAGTATAA AATTACCTTA 
AAATATCCAC ACTATTTCCC TGTCATGAAG AAATGTTGTA TCCCTGAAAC CAGAAGAAGG 
ATGGAAATGG CTTTTAATAC AAGGTGCAAA GAGGAAAACA CCATAATTTT GCAGCAGCTA 
CTCCCACTGC GAACCAAGGT GGCCAAACTA CTCGGTTATA GCACACATGC TGACTTCGTC 
CTTGAAATGA ACACTGCAAA GAGCACAAGC CGCGTAACAG CCTTTCTAGA TGATTTAAGC 
CAGAAGTTAA AACCCTTGGG TGAAGCAGAA CGAGAGTTTA TTTTGAATTT GAAGAAAAAG 
GAATGCAAAG ACAGGGGTTT TGAATATGAT GGGAAAATCA ATGCCTGGGA TCTATATTAC 
TACATGACTC AGACAGAGGA ACTCAAGTAT TCCATAGACC AAGAGTTCCT CAAGGAATAC 
TTCCCAATTG AGGTGGTCAC TGAAGGCTTG CTGAACACCT ACCAGGAGTT GTTGGGACTT 
TCATTTGAAC AAATGACAGA TGCTCATGTT TGGAACAAGA GTGTTACACT TTATACTGTG 
AAGGATAAAG CTACAGGAGA AGTATTGGGA CAGTTCTATT TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ATCATGCGGC CTGCTTCGGT CTCCAGCCTG GCTGCCTTCT GCCTGATGGA 
AGCCGGATGA TGGCAGTGGC TGCCCTCGTG GTGAACTTCT CACAGCCAGT GGCAGGTCGT 
CCCTCTCTCC TGAGACACGA CGAGACTGAT TTTGCACGAT TTAGCGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCGCAAATG CTTGAAAATT GGGTGTGGGA CGTCGATTCC 
CTCCGAAGAT TGTCAAAACA TTATAAAGAT GGAAGCCCTA TTGCAGACGA TCTGCTTGAA 
AAACTTGTTG CTTCTAGGCT GGTCAACACA GGTCTTCTGA CCCTGCGCCA GATTGTTTTG 
AGCAAAGTTG ATCAGTCTCT TCATACCAAC ACATCGCTGG ATGCTGCAAG TGAATATGCC 
AAATACTGCT CAGAAATATT AGGAGTTGCA GCTACTCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGGGG ATACGATGGC CAATATTATG GATATCTTTG GAGTGAAGTA 
TTTTCCATGG ATATGTTTTA CAGCTGTTTT AAAAAAGAAG GGATAATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAA CCTGGGGGAT CTCTGGACGG CATGGACATG 
CTCCACAATT TCTTGAAACG TGAGCCAAAC CAAAAAGCGT TCCTAATGAG TAGAGGCCTG 
CATGCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLN ( NM_020726.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065777.1 Homo sapiens neurolysin (NLN), mRNA.

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CloneID	OHu10904
Clone ID Related Accession (Same CDS sequence)	NM_020726.4 , NM_020726.5
Accession Version	NM_020726.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	neurolysin, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB992921.1, AJ300837.1, AC016643.6 and CA441264.1. On Jun 8, 2010 this sequence version replaced NM_020726.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB033052.2, SRR1803615.18418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGGTGAGGA CTTACTTTCA TGAGTTTGGT 
CACGTGATGC ATCAGATTTG TGCACAGACT GATTTTGCAC GATTTAGCGG AACAAATGTG 
GAAACTGACT TTGTAGAGGT GCCATCGCAA ATGCTTGAAA ATTGGGTGTG GGACGTCGAT 
TCCCTCCGAA GATTGTCAAA ACATTATAAA GATGGAAGCC CTATTGCAGA CGATCTGCTT 
GAAAAACTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACCCTGCG CCAGATTGTT 
TTGAGCAAAG TTGATCAGTC TCTTCATACC AACACATCGC TGGATGCTGC AAGTGAATAT 
GCCAAATACT GCTCAGAAAT ATTAGGAGTT GCAGCTACTC CAGGCACAAA TATGCCAGCT 
ACCTTTGGAC ATTTGGCAGG GGGATACGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTATTTTCCA TGGATATGTT TTACAGCTGT TTTAAAAAAG AAGGGATAAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CCTAATCCTG AAACCTGGGG GATCTCTGGA CGGCATGGAC 
ATGCTCCACA ATTTCTTGAA ACGTGAGCCA AACCAAAAAG CGTTCCTAAT GAGTAGAGGC 
CTGCATGCTC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065777.1
CDS	179..2293
Translation	MIARCLLAVRSLRRVGGSRILLRMTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Target ORF information:

RefSeq Version	NM_020726.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neurolysin (NLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020726.4

ORF Insert Sequence:

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGGTGAGGA CTTACTTTCA TGAGTTTGGT 
CACGTGATGC ATCAGATTTG TGCACAGACT GATTTTGCAC GATTTAGCGG AACAAATGTG 
GAAACTGACT TTGTAGAGGT GCCATCGCAA ATGCTTGAAA ATTGGGTGTG GGACGTCGAT 
TCCCTCCGAA GATTGTCAAA ACATTATAAA GATGGAAGCC CTATTGCAGA CGATCTGCTT 
GAAAAACTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACCCTGCG CCAGATTGTT 
TTGAGCAAAG TTGATCAGTC TCTTCATACC AACACATCGC TGGATGCTGC AAGTGAATAT 
GCCAAATACT GCTCAGAAAT ATTAGGAGTT GCAGCTACTC CAGGCACAAA TATGCCAGCT 
ACCTTTGGAC ATTTGGCAGG GGGATACGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTATTTTCCA TGGATATGTT TTACAGCTGT TTTAAAAAAG AAGGGATAAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CCTAATCCTG AAACCTGGGG GATCTCTGGA CGGCATGGAC 
ATGCTCCACA ATTTCTTGAA ACGTGAGCCA AACCAAAAAG CGTTCCTAAT GAGTAGAGGC 
CTGCATGCTC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLN ( NM_020726.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065777.1 Homo sapiens neurolysin (NLN), mRNA.

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CloneID	OHu10904
Clone ID Related Accession (Same CDS sequence)	NM_020726.4 , NM_020726.5
Accession Version	NM_020726.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571932800000
Organism	Homo sapiens(human)
Product	neurolysin, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB992921.1, AJ300837.1, AC016643.6 and CA441264.1. On Nov 23, 2018 this sequence version replaced NM_020726.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB033052.2, SRR1803615.18418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000380985.10/ ENSP00000370372.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGGTGAGGA CTTACTTTCA TGAGTTTGGT 
CACGTGATGC ATCAGATTTG TGCACAGACT GATTTTGCAC GATTTAGCGG AACAAATGTG 
GAAACTGACT TTGTAGAGGT GCCATCGCAA ATGCTTGAAA ATTGGGTGTG GGACGTCGAT 
TCCCTCCGAA GATTGTCAAA ACATTATAAA GATGGAAGCC CTATTGCAGA CGATCTGCTT 
GAAAAACTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACCCTGCG CCAGATTGTT 
TTGAGCAAAG TTGATCAGTC TCTTCATACC AACACATCGC TGGATGCTGC AAGTGAATAT 
GCCAAATACT GCTCAGAAAT ATTAGGAGTT GCAGCTACTC CAGGCACAAA TATGCCAGCT 
ACCTTTGGAC ATTTGGCAGG GGGATACGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTATTTTCCA TGGATATGTT TTACAGCTGT TTTAAAAAAG AAGGGATAAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CCTAATCCTG AAACCTGGGG GATCTCTGGA CGGCATGGAC 
ATGCTCCACA ATTTCTTGAA ACGTGAGCCA AACCAAAAAG CGTTCCTAAT GAGTAGAGGC 
CTGCATGCTC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065777.1
CDS	170..2284
Translation	MIARCLLAVRSLRRVGGSRILLRMTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Target ORF information:

RefSeq Version	NM_020726.5
Organism	Homo sapiens(human)
Definition	Homo sapiens neurolysin (NLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020726.5

ORF Insert Sequence:

ATGATCGCCC GGTGCCTTTT GGCTGTGCGA AGCCTCCGCA GAGTTGGTGG TTCCAGGATT 
TTACTCAGAA TGACGTTAGG AAGAGAAGTG ATGTCTCCTC TTCAGGCAAT GTCTTCCTAT 
ACTGTGGCTG GCAGAAATGT TTTAAGATGG GATCTTTCAC CAGAGCAAAT TAAAACAAGA 
ACTGAGGAGC TCATTGTGCA GACCAAACAG GTGTACGATG CTGTTGGAAT GCTCGGTATT 
GAGGAAGTAA CTTACGAGAA CTGTCTGCAG GCACTGGCAG ATGTAGAAGT AAAGTATATA 
GTGGAAAGGA CCATGCTAGA CTTTCCCCAG CATGTATCCT CTGACAAAGA AGTACGAGCA 
GCAAGTACAG AAGCAGACAA AAGACTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGGA 
GATATATTTG AGAGAATTGT TCATTTACAG GAAACCTGTG ATCTGGGGAA GATAAAACCT 
GAGGCCAGAC GATACTTGGA AAAGTCAATT AAAATGGGGA AAAGAAATGG GCTCCATCTT 
CCTGAACAAG TACAGAATGA AATCAAATCA ATGAAGAAAA GAATGAGTGA GCTATGTATT 
GATTTTAACA AAAACCTCAA TGAGGATGAT ACCTTCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGATTT CATTGACAGT TTAGAAAAGA CAGATGATGA CAAGTATAAA 
ATTACCTTAA AATATCCACA CTATTTCCCT GTCATGAAGA AATGTTGTAT CCCTGAAACC 
AGAAGAAGGA TGGAAATGGC TTTTAATACA AGGTGCAAAG AGGAAAACAC CATAATTTTG 
CAGCAGCTAC TCCCACTGCG AACCAAGGTG GCCAAACTAC TCGGTTATAG CACACATGCT 
GACTTCGTCC TTGAAATGAA CACTGCAAAG AGCACAAGCC GCGTAACAGC CTTTCTAGAT 
GATTTAAGCC AGAAGTTAAA ACCCTTGGGT GAAGCAGAAC GAGAGTTTAT TTTGAATTTG 
AAGAAAAAGG AATGCAAAGA CAGGGGTTTT GAATATGATG GGAAAATCAA TGCCTGGGAT 
CTATATTACT ACATGACTCA GACAGAGGAA CTCAAGTATT CCATAGACCA AGAGTTCCTC 
AAGGAATACT TCCCAATTGA GGTGGTCACT GAAGGCTTGC TGAACACCTA CCAGGAGTTG 
TTGGGACTTT CATTTGAACA AATGACAGAT GCTCATGTTT GGAACAAGAG TGTTACACTT 
TATACTGTGA AGGATAAAGC TACAGGAGAA GTATTGGGAC AGTTCTATTT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA TCATGCGGCC TGCTTCGGTC TCCAGCCTGG CTGCCTTCTG 
CCTGATGGAA GCCGGATGAT GGCAGTGGCT GCCCTCGTGG TGAACTTCTC ACAGCCAGTG 
GCAGGTCGTC CCTCTCTCCT GAGACACGAC GAGGTGAGGA CTTACTTTCA TGAGTTTGGT 
CACGTGATGC ATCAGATTTG TGCACAGACT GATTTTGCAC GATTTAGCGG AACAAATGTG 
GAAACTGACT TTGTAGAGGT GCCATCGCAA ATGCTTGAAA ATTGGGTGTG GGACGTCGAT 
TCCCTCCGAA GATTGTCAAA ACATTATAAA GATGGAAGCC CTATTGCAGA CGATCTGCTT 
GAAAAACTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACCCTGCG CCAGATTGTT 
TTGAGCAAAG TTGATCAGTC TCTTCATACC AACACATCGC TGGATGCTGC AAGTGAATAT 
GCCAAATACT GCTCAGAAAT ATTAGGAGTT GCAGCTACTC CAGGCACAAA TATGCCAGCT 
ACCTTTGGAC ATTTGGCAGG GGGATACGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTATTTTCCA TGGATATGTT TTACAGCTGT TTTAAAAAAG AAGGGATAAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CCTAATCCTG AAACCTGGGG GATCTCTGGA CGGCATGGAC 
ATGCTCCACA ATTTCTTGAA ACGTGAGCCA AACCAAAAAG CGTTCCTAAT GAGTAGAGGC 
CTGCATGCTC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanism of Peptide Binding and Cleavage by the Human Mitochondrial Peptidase Neurolysin.
Journal of molecular biology430(3)348-362(2018 02)
Teixeira PF,Masuyer G,Pinho CM,Branca RMM,Kmiec B,Wallin C,Wärmländer SKTS,Berntsson RP,Ankarcrona M,Gräslund A,Lehtiö J,Stenmark P,Glaser E

Genome-wide association study for refractive astigmatism reveals genetic co-determination with spherical equivalent refractive error: the CREAM consortium.
Human genetics134(2)131-46(2015 Feb)
Li Q,Wojciechowski R,Simpson CL,Hysi PG,Verhoeven VJ,Ikram MK,Höhn R,Vitart V,Hewitt AW,Oexle K,Mäkelä KM,MacGregor S,Pirastu M,Fan Q,Cheng CY,St Pourcain B,McMahon G,Kemp JP,Northstone K,Rahi JS,Cumberland PM,Martin NG,Sanfilippo PG,Lu Y,Wang YX,Hayward C,Polašek O,Campbell H,Bencic G,Wright AF,Wedenoja J,Zeller T,Schillert A,Mirshahi A,Lackner K,Yip SP,Yap MK,Ried JS,Gieger C,Murgia F,Wilson JF,Fleck B,Yazar S,Vingerling JR,Hofman A,Uitterlinden A,Rivadeneira F,Amin N,Karssen L,Oostra BA,Zhou X,Teo YY,Tai ES,Vithana E,Barathi V,Zheng Y,Siantar RG,Neelam K,Shin Y,Lam J,Yonova-Doing E,Venturini C,Hosseini SM,Wong HS,Lehtimäki T,Kähönen M,Raitakari O,Timpson NJ,Evans DM,Khor CC,Aung T,Young TL,Mitchell P,Klein B,van Duijn CM,Meitinger T,Jonas JB,Baird PN,Mackey DA,Wong TY,Saw SM,Pärssinen O,Stambolian D,Hammond CJ,Klaver CC,Williams C,Paterson AD,Bailey-Wilson JE,Guggenheim JA,

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Swapping the substrate specificities of the neuropeptidases neurolysin and thimet oligopeptidase.
The Journal of biological chemistry282(13)9722-32(2007 Mar)
Lim EJ,Sampath S,Coll-Rodriguez J,Schmidt J,Ray K,Rodgers DW

Endopeptidases 3.4.24.15 and 24.16 in endothelial cells: potential role in vasoactive peptide metabolism.
American journal of physiology. Heart and circulatory physiology284(6)H1978-84(2003 Jun)
Norman MU,Reeve SB,Dive V,Smith AI,Lew RA

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NLN cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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