PCK2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001018073.2	NP_001018083.2	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 2 precursor
NM_001291556.1	NP_001278485.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
NM_004563.3	NP_004554.3	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 1 precursor
NM_001308054.1	NP_001294983.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
NM_001018073.3	NP_001018083.2	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 2 precursor
XM_006720158.3	XP_006720221.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform X1
NM_004563.4	NP_004554.3	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 1 precursor
NM_001291556.2	NP_001278485.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
NM_001308054.2	NP_001294983.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
XM_006720158.2	XP_006720221.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27947	NM_001018073.3 Latest version!	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu47725	XM_006720158.3 Latest version!	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu27941	NM_001308054.2 Latest version!	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu27941	NM_001291556.2 Latest version!	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu27958	NM_004563.4 Latest version!	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu27947	NM_001018073.2	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu27958	NM_004563.3	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu27941	NM_001291556.1	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu27941	NM_001308054.1	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu47725	XM_006720158.2	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

PCK2 ( NM_001018073.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018083.2 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27947
Clone ID Related Accession (Same CDS sequence)	NM_001018073.2 , NM_001018073.3
Accession Version	NM_001018073.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY022221.1, DB028389.1, AL136295.3, BX248272.1, KF455834.1 and CN359636.1. On Apr 19, 2014 this sequence version replaced NM_001018073.1. Transcript Variant: This variant (2) lacks multiple 3' coding exons and its 3' terminal exon extends past a splice site used in the variant 1, resulting in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.425053.1, BX248272.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTATGT GCGGTGGGGA AGGTGTGGCA 
CAGCCTCCAG GCCTCAGCAC CTTAATGGTG GAAAAGCTTT CTCCACAACC TCCAACCATC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018083.2
CDS	276..1601
Translation	MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGMCGGEGVAQPPGLSTLMVEKLSPQPPTIF

Target ORF information:

RefSeq Version	NM_001018073.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018073.2

ORF Insert Sequence:

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTATGT GCGGTGGGGA AGGTGTGGCA 
CAGCCTCCAG GCCTCAGCAC CTTAATGGTG GAAAAGCTTT CTCCACAACC TCCAACCATC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( NM_001018073.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018083.2 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27947
Clone ID Related Accession (Same CDS sequence)	NM_001018073.2 , NM_001018073.3
Accession Version	NM_001018073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559145600000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028389.1, AL136295.3, BX248272.1, KF455834.1 and CN359636.1. On May 31, 2019 this sequence version replaced NM_001018073.2. Transcript Variant: This variant (2) lacks multiple 3' coding exons and its 3' terminal exon extends past a splice site used in the variant 1, resulting in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.141116.1, BX248272.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTATGT GCGGTGGGGA AGGTGTGGCA 
CAGCCTCCAG GCCTCAGCAC CTTAATGGTG GAAAAGCTTT CTCCACAACC TCCAACCATC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018083.2
CDS	96..1421
Translation	MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGMCGGEGVAQPPGLSTLMVEKLSPQPPTIF

Target ORF information:

RefSeq Version	NM_001018073.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018073.3

ORF Insert Sequence:

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTATGT GCGGTGGGGA AGGTGTGGCA 
CAGCCTCCAG GCCTCAGCAC CTTAATGGTG GAAAAGCTTT CTCCACAACC TCCAACCATC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( XM_006720158.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720221.1 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu47725
Clone ID Related Accession (Same CDS sequence)	XM_006720158.3 , XM_006720158.2
Accession Version	XM_006720158.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720158.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGA CTTCTGGGAA CAGGAGGTTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720221.1
CDS	96..1589
Translation	MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGLLGTGGS

Target ORF information:

RefSeq Version	XM_006720158.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720158.3

ORF Insert Sequence:

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGA CTTCTGGGAA CAGGAGGTTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( XM_006720158.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720221.1 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.

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CloneID	OHu47725
Clone ID Related Accession (Same CDS sequence)	XM_006720158.3 , XM_006720158.2
Accession Version	XM_006720158.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006720158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGA CTTCTGGGAA CAGGAGGTTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720221.1
CDS	269..1762
Translation	MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGLLGTGGS

Target ORF information:

RefSeq Version	XM_006720158.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720158.2

ORF Insert Sequence:

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGA CTTCTGGGAA CAGGAGGTTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( NM_001291556.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278485.1 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 3, mRNA.

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CloneID	OHu27941
Clone ID Related Accession (Same CDS sequence)	NM_001291556.1 , NM_001308054.1 , NM_001291556.2 , NM_001308054.2
Accession Version	NM_001291556.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1538928000000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY022221.1, DB028389.1, AK316206.1, AK129934.1 and CA444720.1. Transcript Variant: This variant (3) contains an alternate exon and lacks an exon in the 5' region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Variants 3 and 4 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278485.1
CDS	512..2032
Translation	MSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Target ORF information:

RefSeq Version	NM_001291556.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291556.1

ORF Insert Sequence:

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( NM_001308054.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294983.1 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 4, mRNA.

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CloneID	OHu27941
Clone ID Related Accession (Same CDS sequence)	NM_001291556.1 , NM_001308054.1 , NM_001291556.2 , NM_001308054.2
Accession Version	NM_001308054.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1538928000000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA645036.1, BQ957219.1, AK301400.1, BU153045.1 and CA444720.1. On Apr 21, 2015 this sequence version replaced XM_011536813.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate 5'-terminal exon, which results in use of a downstream in-frame start codon compared to variant 1. It encodes isoform 3, which has a shorter N-terminus than isoform 1. Variants 3 and 4 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.254301.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 5' end.

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294983.1
CDS	939..2459
Translation	MSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Target ORF information:

RefSeq Version	NM_001308054.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308054.1

ORF Insert Sequence:

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( NM_001291556.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278485.1 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 3, mRNA.

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CloneID	OHu27941
Clone ID Related Accession (Same CDS sequence)	NM_001291556.1 , NM_001308054.1 , NM_001291556.2 , NM_001308054.2
Accession Version	NM_001291556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559145600000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028389.1, AK316206.1 and AK129934.1. On May 31, 2019 this sequence version replaced NM_001291556.1. Transcript Variant: This variant (3) contains an alternate exon and lacks an exon in the 5' region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Variants 3 and 4 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278485.1
CDS	332..1852
Translation	MSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Target ORF information:

RefSeq Version	NM_001291556.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291556.2

ORF Insert Sequence:

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( NM_001308054.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294983.1 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 4, mRNA.

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CloneID	OHu27941
Clone ID Related Accession (Same CDS sequence)	NM_001291556.1 , NM_001308054.1 , NM_001291556.2 , NM_001308054.2
Accession Version	NM_001308054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559232000000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA645036.1, BQ957219.1, AK301400.1, BU153045.1 and CA444720.1. On Jun 1, 2019 this sequence version replaced NM_001308054.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate 5'-terminal exon, which results in use of a downstream in-frame start codon compared to variant 1. It encodes isoform 3, which has a shorter N-terminus than isoform 1. Variants 3 and 4 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.254301.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 5' end.

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294983.1
CDS	909..2429
Translation	MSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Target ORF information:

RefSeq Version	NM_001308054.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308054.2

ORF Insert Sequence:

ATGTCCCCAG CTGATTTCCA GCGAGCTGTG GATGAGAGGT TTCCAGGCTG CATGCAGGGC 
CGCACCATGT ATGTGCTTCC ATTCAGCATG GGTCCTGTGG GCTCCCCGCT GTCCCGCATC 
GGGGTGCAGC TCACTGACTC AGCCTATGTG GTGGCAAGCA TGCGTATTAT GACCCGACTG 
GGGACACCTG TGCTTCAGGC CCTGGGAGAT GGTGACTTTG TCAAGTGTCT GCACTCCGTG 
GGCCAGCCCC TGACAGGACA AGGGGAGCCA GTGAGCCAGT GGCCGTGCAA CCCAGAGAAA 
ACCCTGATTG GCCACGTGCC CGACCAGCGG GAGATCATCT CCTTCGGCAG CGGCTATGGT 
GGCAACTCCC TGCTGGGCAA GAAGTGCTTT GCCCTACGCA TCGCCTCTCG GCTGGCCCGG 
GATGAGGGCT GGCTGGCAGA GCACATGCTG ATCCTGGGCA TCACCAGCCC TGCAGGGAAG 
AAGCGCTATG TGGCAGCCGC CTTCCCTAGT GCCTGTGGCA AGACCAACCT GGCTATGATG 
CGGCCTGCAC TGCCAGGCTG GAAAGTGGAG TGTGTGGGGG ATGATATTGC TTGGATGAGG 
TTTGACAGTG AAGGTCGACT CCGGGCCATC AACCCTGAGA ACGGCTTCTT TGGGGTTGCC 
CCTGGTACCT CTGCCACCAC CAATCCCAAC GCCATGGCTA CAATCCAGAG TAACACTATT 
TTTACCAATG TGGCTGAGAC CAGTGATGGT GGCGTGTACT GGGAGGGCAT TGACCAGCCT 
CTTCCACCTG GTGTTACTGT GACCTCCTGG CTGGGCAAAC CCTGGAAACC TGGTGACAAG 
GAGCCCTGTG CACATCCCAA CTCTCGATTT TGTGCCCCGG CTCGCCAGTG CCCCATCATG 
GACCCAGCCT GGGAGGCCCC AGAGGGTGTC CCCATTGACG CCATCATCTT TGGTGGCCGC 
AGACCCAAAG GGGTACCCCT GGTATACGAG GCCTTCAACT GGCGTCATGG GGTGTTTGTG 
GGCAGCGCCA TGCGCTCTGA GTCCACTGCT GCAGCAGAAC ACAAAGGGAA GATCATCATG 
CACGACCCAT TTGCCATGCG GCCCTTTTTT GGCTACAACT TCGGGCACTA CCTGGAACAC 
TGGCTGAGCA TGGAAGGGCG CAAGGGGGCC CAGCTGCCCC GTATCTTCCA TGTCAACTGG 
TTCCGGCGTG ACGAGGCAGG GCACTTCCTG TGGCCAGGCT TTGGGGAGAA TGCTCGGGTG 
CTAGACTGGA TCTGCCGGCG GTTAGAGGGG GAGGACAGTG CCCGAGAGAC ACCCATTGGG 
CTGGTGCCAA AGGAAGGAGC CTTGGATCTC AGCGGCCTCA GAGCTATAGA CACCACTCAG 
CTGTTCTCCC TCCCCAAGGA CTTCTGGGAA CAGGAGGTTC GTGACATTCG GAGCTACCTG 
ACAGAGCAGG TCAACCAGGA TCTGCCCAAA GAGGTGTTGG CTGAGCTTGA GGCCCTGGAG 
AGACGTGTGC ACAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( NM_004563.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004554.3 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 1, mRNA.

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CloneID	OHu27958
Clone ID Related Accession (Same CDS sequence)	NM_004563.3 , NM_004563.4
Accession Version	NM_004563.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY022221.1, DB028389.1, AK129934.1 and CA444720.1. This sequence is a reference standard in the RefSeqGene project. On Apr 19, 2014 this sequence version replaced NM_004563.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.213953.1, AK129934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGG AAGATCATCA TGCACGACCC ATTTGCCATG 
CGGCCCTTTT TTGGCTACAA CTTCGGGCAC TACCTGGAAC ACTGGCTGAG CATGGAAGGG 
CGCAAGGGGG CCCAGCTGCC CCGTATCTTC CATGTCAACT GGTTCCGGCG TGACGAGGCA 
GGGCACTTCC TGTGGCCAGG CTTTGGGGAG AATGCTCGGG TGCTAGACTG GATCTGCCGG 
CGGTTAGAGG GGGAGGACAG TGCCCGAGAG ACACCCATTG GGCTGGTGCC AAAGGAAGGA 
GCCTTGGATC TCAGCGGCCT CAGAGCTATA GACACCACTC AGCTGTTCTC CCTCCCCAAG 
GACTTCTGGG AACAGGAGGT TCGTGACATT CGGAGCTACC TGACAGAGCA GGTCAACCAG 
GATCTGCCCA AAGAGGTGTT GGCTGAGCTT GAGGCCCTGG AGAGACGTGT GCACAAAATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004554.3
CDS	276..2198
Translation	MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Target ORF information:

RefSeq Version	NM_004563.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004563.3

ORF Insert Sequence:

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGG AAGATCATCA TGCACGACCC ATTTGCCATG 
CGGCCCTTTT TTGGCTACAA CTTCGGGCAC TACCTGGAAC ACTGGCTGAG CATGGAAGGG 
CGCAAGGGGG CCCAGCTGCC CCGTATCTTC CATGTCAACT GGTTCCGGCG TGACGAGGCA 
GGGCACTTCC TGTGGCCAGG CTTTGGGGAG AATGCTCGGG TGCTAGACTG GATCTGCCGG 
CGGTTAGAGG GGGAGGACAG TGCCCGAGAG ACACCCATTG GGCTGGTGCC AAAGGAAGGA 
GCCTTGGATC TCAGCGGCCT CAGAGCTATA GACACCACTC AGCTGTTCTC CCTCCCCAAG 
GACTTCTGGG AACAGGAGGT TCGTGACATT CGGAGCTACC TGACAGAGCA GGTCAACCAG 
GATCTGCCCA AAGAGGTGTT GGCTGAGCTT GAGGCCCTGG AGAGACGTGT GCACAAAATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( NM_004563.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004554.3 Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 1, mRNA.

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CloneID	OHu27958
Clone ID Related Accession (Same CDS sequence)	NM_004563.3 , NM_004563.4
Accession Version	NM_004563.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571932800000
Organism	Homo sapiens(human)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028389.1 and AK129934.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004563.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.832540.1, GQ891518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000216780.9/ ENSP00000216780.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGG AAGATCATCA TGCACGACCC ATTTGCCATG 
CGGCCCTTTT TTGGCTACAA CTTCGGGCAC TACCTGGAAC ACTGGCTGAG CATGGAAGGG 
CGCAAGGGGG CCCAGCTGCC CCGTATCTTC CATGTCAACT GGTTCCGGCG TGACGAGGCA 
GGGCACTTCC TGTGGCCAGG CTTTGGGGAG AATGCTCGGG TGCTAGACTG GATCTGCCGG 
CGGTTAGAGG GGGAGGACAG TGCCCGAGAG ACACCCATTG GGCTGGTGCC AAAGGAAGGA 
GCCTTGGATC TCAGCGGCCT CAGAGCTATA GACACCACTC AGCTGTTCTC CCTCCCCAAG 
GACTTCTGGG AACAGGAGGT TCGTGACATT CGGAGCTACC TGACAGAGCA GGTCAACCAG 
GATCTGCCCA AAGAGGTGTT GGCTGAGCTT GAGGCCCTGG AGAGACGTGT GCACAAAATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004554.3
CDS	96..2018
Translation	MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Target ORF information:

RefSeq Version	NM_004563.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004563.4

ORF Insert Sequence:

ATGGCCGCAT TGTACCGCCC TGGCCTGCGG CTTAACTGGC ATGGGCTGAG CCCCTTGGGC 
TGGCCATCAT GCCGTAGCAT CCAGACCCTG CGAGTGCTTA GTGGAGATCT GGGCCAGCTT 
CCCACTGGCA TTCGAGATTT TGTAGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGAACTGA GGCTGAGAAT ACTGCCACAC TGACCCTGCT GGAGCAGCAG 
GGCCTCATCC GAAAGCTCCC CAAGTACAAT AACTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAGCG GGACACGGTA 
CCACTCCCGC CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGATTTC 
CAGCGAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCAGG GCCGCACCAT GTATGTGCTT 
CCATTCAGCA TGGGTCCTGT GGGCTCCCCG CTGTCCCGCA TCGGGGTGCA GCTCACTGAC 
TCAGCCTATG TGGTGGCAAG CATGCGTATT ATGACCCGAC TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGTGACTT TGTCAAGTGT CTGCACTCCG TGGGCCAGCC CCTGACAGGA 
CAAGGGGAGC CAGTGAGCCA GTGGCCGTGC AACCCAGAGA AAACCCTGAT TGGCCACGTG 
CCCGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTACG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCA 
GAGCACATGC TGATCCTGGG CATCACCAGC CCTGCAGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCTA GTGCCTGTGG CAAGACCAAC CTGGCTATGA TGCGGCCTGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGATATT GCTTGGATGA GGTTTGACAG TGAAGGTCGA 
CTCCGGGCCA TCAACCCTGA GAACGGCTTC TTTGGGGTTG CCCCTGGTAC CTCTGCCACC 
ACCAATCCCA ACGCCATGGC TACAATCCAG AGTAACACTA TTTTTACCAA TGTGGCTGAG 
ACCAGTGATG GTGGCGTGTA CTGGGAGGGC ATTGACCAGC CTCTTCCACC TGGTGTTACT 
GTGACCTCCT GGCTGGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCACATCCC 
AACTCTCGAT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TGGACCCAGC CTGGGAGGCC 
CCAGAGGGTG TCCCCATTGA CGCCATCATC TTTGGTGGCC GCAGACCCAA AGGGGTACCC 
CTGGTATACG AGGCCTTCAA CTGGCGTCAT GGGGTGTTTG TGGGCAGCGC CATGCGCTCT 
GAGTCCACTG CTGCAGCAGA ACACAAAGGG AAGATCATCA TGCACGACCC ATTTGCCATG 
CGGCCCTTTT TTGGCTACAA CTTCGGGCAC TACCTGGAAC ACTGGCTGAG CATGGAAGGG 
CGCAAGGGGG CCCAGCTGCC CCGTATCTTC CATGTCAACT GGTTCCGGCG TGACGAGGCA 
GGGCACTTCC TGTGGCCAGG CTTTGGGGAG AATGCTCGGG TGCTAGACTG GATCTGCCGG 
CGGTTAGAGG GGGAGGACAG TGCCCGAGAG ACACCCATTG GGCTGGTGCC AAAGGAAGGA 
GCCTTGGATC TCAGCGGCCT CAGAGCTATA GACACCACTC AGCTGTTCTC CCTCCCCAAG 
GACTTCTGGG AACAGGAGGT TCGTGACATT CGGAGCTACC TGACAGAGCA GGTCAACCAG 
GATCTGCCCA AAGAGGTGTT GGCTGAGCTT GAGGCCCTGG AGAGACGTGT GCACAAAATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PCK2
Entrez Gene ID	5106
Full Name	phosphoenolpyruvate carboxykinase 2, mitochondrial
Synonyms	PEPCK,PEPCK-M,PEPCK2
General protein information	Preferred Names phosphoenolpyruvate carboxykinase 2, mitochondrial Names phosphoenolpyruvate carboxykinase [GTP], mitochondrial PEP carboxykinase phosphopyruvate carboxylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2-q12
Summary	This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014].
Disorder MIM:	614095
Disorder Html:	PEPCK deficiency, mitochondrial, 261650 (1)

Macaca mulatta (Rhesus monkey)	PCK2	XP_001111487.1
Canis lupus familiaris (dog)	PCK2	XP_537379.2
Mus musculus (house mouse)	Pck2	NP_083270.1
Rattus norvegicus (Norway rat)	Pck2	NP_001101847.2
Homo sapiens (human)	PCK2	NP_004554.2
Drosophila melanogaster (fruit fly)	Pepck	NP_523784.2
Caenorhabditis elegans (roundworm)	pck-1	NP_497134.1
Pan troglodytes (chimpanzee)	PCK2	XP_001165863.1
Bos taurus (cattle)	PCK2	NP_001192523.1
Danio rerio (zebrafish)	pck2	NP_998357.1
Xenopus tropicalis (tropical clawed frog)	pck2	NP_001034816.1
Gallus gallus (chicken)	PCK2	NP_990801.1
Caenorhabditis elegans (roundworm)	pck-2	NP_001021587.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003350	XP_003436452.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PCK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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