PCK2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCK2
Entrez Gene ID	396457
Full Name	phosphoenolpyruvate carboxykinase 2, mitochondrial
Synonyms	PEPCK-M
General protein information	Preferred Names phosphoenolpyruvate carboxykinase 2, mitochondrial Names phosphoenolpyruvate carboxykinase [GTP], mitochondrial phosphoenolpyruvate carboxylase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205470.1	NP_990801.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial precursor

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PCK2	XP_001111487.1
Canis lupus familiaris (dog)	PCK2	XP_537379.2
Mus musculus (house mouse)	Pck2	NP_083270.1
Rattus norvegicus (Norway rat)	Pck2	NP_001101847.2
Homo sapiens (human)	PCK2	NP_004554.2
Drosophila melanogaster (fruit fly)	Pepck	NP_523784.2
Caenorhabditis elegans (roundworm)	pck-1	NP_497134.1
Pan troglodytes (chimpanzee)	PCK2	XP_001165863.1
Bos taurus (cattle)	PCK2	NP_001192523.1
Danio rerio (zebrafish)	pck2	NP_998357.1
Xenopus tropicalis (tropical clawed frog)	pck2	NP_001034816.1
Gallus gallus (chicken)	PCK2	NP_990801.1
Caenorhabditis elegans (roundworm)	pck-2	NP_001021587.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003350	XP_003436452.1

Gene Ontology
Bibliography

Related articles in PubMed

Aryl hydrocarbon receptor activation by dioxin targets phosphoenolpyruvate carboxykinase (PEPCK) for ADP-ribosylation via 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly(ADP-ribose) polymerase (TiPARP).
Diani-Moore S, Zhang S, Ram P, Rifkind AB
The Journal of biological chemistry288(30)21514-25(2013 Jul)

Structural insights into the mechanism of PEPCK catalysis.
Holyoak T, Sullivan SM, Nowak T
Biochemistry45(27)8254-63(2006 Jul)

Alleles of cytosolic phosphoenolpyruvate carboxykinase (PEPCK): trait association and interaction with mitochondrial PEPCK in a strain of White Leghorn chickens.
Parsanejad R, Torkamanzehi A, Zadworny D, Kuhnlein U
Poultry science82(11)1708-15(2003 Nov)

Mitochondrial PEPCK: a highly polymorphic gene with alleles co-selected with Marek's disease resistance in chickens.
Li S, Zadworny D, Aggrey SE, Kuhnlein U
Animal genetics29(5)395-7(1998 Oct)

The promoter regulatory regions of the genes for the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken and the rat have different species-specific roles in gluconeogenesis.
Savon SP, Hakimi P, Crawford DR, Klemm DJ, Gurney AL, Hanson RW
The Journal of nutrition127(2)276-85(1997 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa26845	NM_205470.1 Latest version!	Gallus gallus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCK2 ( NM_205470.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990801.1 Gallus gallus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.

Price & Availability↑

CloneID	OGa26845
Clone ID Related Accession (Same CDS sequence)	NM_205470.1
Accession Version	NM_205470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Gallus gallus(chicken)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05419.1. On Aug 30, 2004 this sequence version replaced XM_423257.1. ##Evidence-Data-START## Transcript exon combination :: J05419.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTTTGGT TAAGAGGGGG GGCGCAGAGT TGTAGGGGGG GGGAAACTGA GGACAGAATG 
CAGCGCGGGA TGTGGGGCGT GGGCCTGGCC CGGCGCAGGC TGAGCACGTC GCTGTCGGCG 
CTGCCGGCGG CCGCGCGGGA TTTCGTGGAG GAGGCGGTCC GGCTGTGCAG GCCCAGGGAG 
GTTCTGCTGT GCGATGGGTC CGAGGAGGAG GGGAAGGAGC TGCTCAGAGG GCTGCAGGAC 
GACGGGGTGC TGCATCCGCT GCCCAAATAC GACAACTGCT GGTTGGCTCG CACCGACCCC 
CGGGACGTGG CTCGGGTGCA AAGCAAGACG GTGTTGGTAA CCCCCGAACA GAGCGACGCC 
GTCCCCCCAC CCCCCCCATC CGGGTCCCCC CAATTGGGGA ACTGGATGAG CCCCAATGCT 
TTCCAGGCAG CTGTGCAGGA GCGTTTCCCC GGATGCATGG CAGGCCGCCC CCTCTACGTC 
ATCCCATTCA GCATGGGCCC CCCCACGTCC CCCTTGGCCA AACTGGGGGT TCAGGTGACC 
GACTCCCCCT ACGTGGTGCT CTCCATGCGC ATTATGACCC GCGTGGGCCC CGCGGTGCTG 
CAGCGCCTCG ACGACGACTT CGTCCGCTGC CTCCACTCTG TGGGGCGGCC TCTGCCCCTC 
ACCGAGCCCC TGGTGAGCTC GTGGCCGTGC GACCGGTCCC GTGTCCTGGT TGCCCACATC 
CCCTCGGAGC GCCGGATCGT CTCCTTCGGT TCGGGATACG GCGGCAATTC GCTGCTGGGC 
AAGAAGTGCT TCGCGCTGGC CATCGCGTCC CGCATGGCCC AGCAGCAGGG CTGGCTGGCC 
GAGCACATGC TGATTTTGGG GGTGACGTCC CCCAGCGGTG AGAAGCGTTA CATGGCGGCG 
GCCTTTCCCA GCGCCTGCGG GAAAACCAAC CTGGCCATGA TGACCCCCAG CCTGCCGGGT 
TGGCGGATCC ACTGCGTTGG GGACGACATT GCGTGGATGA AGTTCGATGA TCGAGGGCGC 
CTCCGCGCCA TCAACCCCGA GCGTGGCTTT TTTGGGGTGG CCCCGGGGAC GTCGTCGCGC 
ACCAACCCCA ACGCCATGGC CACCATCGCC CGCAACACCA TCTTCACCAA CGTGGGGCTG 
CGAAGCGATG GCGGCGTCTA CTGGGACGGC CTGGATGAGC CCACGGAGCC CGGGGTCACC 
TACACCTCCT GGCTGGGCAA GCCGTGGAAG CACGGTGACC CCGAGCCGTG CGCCCACCCC 
AACTCCCGTT TCTGCGCCCC GGCCGATCAG TGCCCCATTA TGGACCCGCG TTGGGACGAC 
CCGGAAGGAG TTCCCATCGA CGCCATCATC TTCGGGGGGC GCCGACCCCG CGGAGTGCCG 
TTGGTGGTGG AGGCCTTTGG GTGGCGCCAC GGAGTTTTCA TGGGCAGCGC AATGAGGAGC 
GAAGCCACCG CCGCCGCCGA GCACAAAGGC GGCCGTTTGA TGCACGACCC CTTCGCCATG 
AGCCCCTTTT TCGGCTACAA CGCGGGGCGT TACCTGGAAC ATTGGCTGTC TACGGGTCTC 
CGGAGCAACG CCCGCCTCCC CCGTCTGTTC CACGTTAATT GGTTCCTCCG AGATAACGAA 
GGTCGCTTCG TTTGGCCCGG CTTCGGTCAC AACGCCCGCG TCTTGGCTTG GATCTTCGGG 
AGGATCCAGG GGAGGGACAC TGCCCGGCCC ACCCCCATCG GTTGGGTACC CAAAGAAGGG 
GATTTGGACC TGGGGGGGCT GCCGGGGGTC GATTACTCCC AACTGTTCCC TATGGAGAAG 
GGCTTTTGGG AGGAGGAGTG CAGGCAGCTG AGGGAGTATT ACGGGGAGAA CTTCGGGGCC 
GATCTGCCCA GGGATGTCAT GGCGGAGCTG GAGGGCCTGG AGGAGAGGGT GAGGAAGATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990801.1
CDS	28..1950
Translation	MFWLRGGAQSCRGGETEDRMQRGMWGVGLARRRLSTSLSALPAAARDFVEEAVRLCRPREVLLCDGSEEEGKELLRGLQDDGVLHPLPKYDNCWLARTDPRDVARVQSKTVLVTPEQSDAVPPPPPSGSPQLGNWMSPNAFQAAVQERFPGCMAGRPLYVIPFSMGPPTSPLAKLGVQVTDSPYVVLSMRIMTRVGPAVLQRLDDDFVRCLHSVGRPLPLTEPLVSSWPCDRSRVLVAHIPSERRIVSFGSGYGGNSLLGKKCFALAIASRMAQQQGWLAEHMLILGVTSPSGEKRYMAAAFPSACGKTNLAMMTPSLPGWRIHCVGDDIAWMKFDDRGRLRAINPERGFFGVAPGTSSRTNPNAMATIARNTIFTNVGLRSDGGVYWDGLDEPTEPGVTYTSWLGKPWKHGDPEPCAHPNSRFCAPADQCPIMDPRWDDPEGVPIDAIIFGGRRPRGVPLVVEAFGWRHGVFMGSAMRSEATAAAEHKGGRLMHDPFAMSPFFGYNAGRYLEHWLSTGLRSNARLPRLFHVNWFLRDNEGRFVWPGFGHNARVLAWIFGRIQGRDTARPTPIGWVPKEGDLDLGGLPGVDYSQLFPMEKGFWEEECRQLREYYGENFGADLPRDVMAELEGLEERVRKM

Target ORF information:

RefSeq Version	NM_205470.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205470.1

ORF Insert Sequence:

ATGTTTTGGT TAAGAGGGGG GGCGCAGAGT TGTAGGGGGG GGGAAACTGA GGACAGAATG 
CAGCGCGGGA TGTGGGGCGT GGGCCTGGCC CGGCGCAGGC TGAGCACGTC GCTGTCGGCG 
CTGCCGGCGG CCGCGCGGGA TTTCGTGGAG GAGGCGGTCC GGCTGTGCAG GCCCAGGGAG 
GTTCTGCTGT GCGATGGGTC CGAGGAGGAG GGGAAGGAGC TGCTCAGAGG GCTGCAGGAC 
GACGGGGTGC TGCATCCGCT GCCCAAATAC GACAACTGCT GGTTGGCTCG CACCGACCCC 
CGGGACGTGG CTCGGGTGCA AAGCAAGACG GTGTTGGTAA CCCCCGAACA GAGCGACGCC 
GTCCCCCCAC CCCCCCCATC CGGGTCCCCC CAATTGGGGA ACTGGATGAG CCCCAATGCT 
TTCCAGGCAG CTGTGCAGGA GCGTTTCCCC GGATGCATGG CAGGCCGCCC CCTCTACGTC 
ATCCCATTCA GCATGGGCCC CCCCACGTCC CCCTTGGCCA AACTGGGGGT TCAGGTGACC 
GACTCCCCCT ACGTGGTGCT CTCCATGCGC ATTATGACCC GCGTGGGCCC CGCGGTGCTG 
CAGCGCCTCG ACGACGACTT CGTCCGCTGC CTCCACTCTG TGGGGCGGCC TCTGCCCCTC 
ACCGAGCCCC TGGTGAGCTC GTGGCCGTGC GACCGGTCCC GTGTCCTGGT TGCCCACATC 
CCCTCGGAGC GCCGGATCGT CTCCTTCGGT TCGGGATACG GCGGCAATTC GCTGCTGGGC 
AAGAAGTGCT TCGCGCTGGC CATCGCGTCC CGCATGGCCC AGCAGCAGGG CTGGCTGGCC 
GAGCACATGC TGATTTTGGG GGTGACGTCC CCCAGCGGTG AGAAGCGTTA CATGGCGGCG 
GCCTTTCCCA GCGCCTGCGG GAAAACCAAC CTGGCCATGA TGACCCCCAG CCTGCCGGGT 
TGGCGGATCC ACTGCGTTGG GGACGACATT GCGTGGATGA AGTTCGATGA TCGAGGGCGC 
CTCCGCGCCA TCAACCCCGA GCGTGGCTTT TTTGGGGTGG CCCCGGGGAC GTCGTCGCGC 
ACCAACCCCA ACGCCATGGC CACCATCGCC CGCAACACCA TCTTCACCAA CGTGGGGCTG 
CGAAGCGATG GCGGCGTCTA CTGGGACGGC CTGGATGAGC CCACGGAGCC CGGGGTCACC 
TACACCTCCT GGCTGGGCAA GCCGTGGAAG CACGGTGACC CCGAGCCGTG CGCCCACCCC 
AACTCCCGTT TCTGCGCCCC GGCCGATCAG TGCCCCATTA TGGACCCGCG TTGGGACGAC 
CCGGAAGGAG TTCCCATCGA CGCCATCATC TTCGGGGGGC GCCGACCCCG CGGAGTGCCG 
TTGGTGGTGG AGGCCTTTGG GTGGCGCCAC GGAGTTTTCA TGGGCAGCGC AATGAGGAGC 
GAAGCCACCG CCGCCGCCGA GCACAAAGGC GGCCGTTTGA TGCACGACCC CTTCGCCATG 
AGCCCCTTTT TCGGCTACAA CGCGGGGCGT TACCTGGAAC ATTGGCTGTC TACGGGTCTC 
CGGAGCAACG CCCGCCTCCC CCGTCTGTTC CACGTTAATT GGTTCCTCCG AGATAACGAA 
GGTCGCTTCG TTTGGCCCGG CTTCGGTCAC AACGCCCGCG TCTTGGCTTG GATCTTCGGG 
AGGATCCAGG GGAGGGACAC TGCCCGGCCC ACCCCCATCG GTTGGGTACC CAAAGAAGGG 
GATTTGGACC TGGGGGGGCT GCCGGGGGTC GATTACTCCC AACTGTTCCC TATGGAGAAG 
GGCTTTTGGG AGGAGGAGTG CAGGCAGCTG AGGGAGTATT ACGGGGAGAA CTTCGGGGCC 
GATCTGCCCA GGGATGTCAT GGCGGAGCTG GAGGGCCTGG AGGAGAGGGT GAGGAAGATG 
TGA