KARS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KARS1
Entrez Gene ID	3735
Full Name	lysyl-tRNA synthetase
Synonyms	CMTRIB,DFNB89,KARS1,KARS2,KRS
General protein information	Preferred Names lysyl-tRNA synthetase Names lysine--tRNA ligase lysRS lysine tRNA ligase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q23.1
Summary	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	601421
Disorder Html:	?Charcot-Marie-Tooth disease, recessive intermediate, B, 613641 (3); Deafness, autosomal recessive 89, 613916 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130089.1	NP_001123561.1	lysine--tRNA ligase isoform 1
XM_017023217.1	XP_016878706.1	lysine--tRNA ligase isoform X1
NM_005548.2	NP_005539.1	lysine--tRNA ligase isoform 2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	krs1	NP_595892.1
Macaca mulatta (Rhesus monkey)	KARS	XP_001104312.2
Neurospora crassa	NCU04020	XP_957635.1
Canis lupus familiaris (dog)	KARS	XP_861533.1
Saccharomyces cerevisiae (baker's yeast)	KRS1	NP_010322.1
Xenopus tropicalis (tropical clawed frog)	kars	NP_001001255.1
Oryza sativa (rice)	Os03g0586800	NP_001050572.1
Homo sapiens (human)	KARS	NP_001123561.1
Mus musculus (house mouse)	Kars	NP_001124340.1
Danio rerio (zebrafish)	kars	NP_001002386.1
Caenorhabditis elegans (roundworm)	kars-1	NP_495453.1
Kluyveromyces lactis	KLLA0F18392g	XP_455904.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007858	XP_317634.3
Eremothecium gossypii	AGOS_AGR037C	NP_986702.1
Bos taurus (cattle)	KARS	NP_001029528.1
Rattus norvegicus (Norway rat)	Kars	NP_001006968.1
Arabidopsis thaliana (thale cress)	ATKRS-1	NP_187777.1
Magnaporthe oryzae (rice blast fungus)	MGG_04966	XP_003712462.1
Pan troglodytes (chimpanzee)	KARS	XP_003315273.1
Gallus gallus (chicken)	KARS	NP_001025754.1
Drosophila melanogaster (fruit fly)	Aats-lys	NP_727353.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000325	XP_310792.5

Gene Ontology
Bibliography

Related articles in PubMed

Biallelic variants in
van der Knaap MS, Bugiani M, Mendes MI, Riley LG, Smith DEC, Rudinger-Thirion J, Frugier M, Breur M, Crawford J, van Gaalen J, Schouten M, Willems M, Waisfisz Q, Mau-Them FT, Rodenburg RJ, Taft RJ, Keren B, Christodoulou J, Depienne C, Simons C, Salomons GS, Mochel F
Neurology92(11)e1225-e1237(2019 03)

Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis.
Nam SH, Kim D, Lee D, Lee HM, Song DG, Jung JW, Kim JE, Kim HJ, Kwon NH, Jo EK, Kim S, Lee JW
The Journal of clinical investigation128(11)5034-5055(2018 11)

Three human aminoacyl-tRNA synthetases have distinct sub-mitochondrial localizations that are unaffected by disease-associated mutations.
González-Serrano LE, Karim L, Pierre F, Schwenzer H, Rötig A, Munnich A, Sissler M
The Journal of biological chemistry293(35)13604-13615(2018 08)

LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau K, Schnoegl S, Garner CC, Wanker EE
Molecular systems biology14(7)e8071(2018 07)

Mutations in KARS cause early-onset hearing loss and leukoencephalopathy: Potential pathogenic mechanism.
Zhou XL, He LX, Yu LJ, Wang Y, Wang XJ, Wang ED, Yang T
Human mutation38(12)1740-1750(2017 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu86026	XM_017023217.1 Latest version!	Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu05050	NM_005548.2 Latest version!	Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu18770	NM_001130089.1 Latest version!	Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KARS1 ( XM_017023217.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878706.1 Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86026
Clone ID Related Accession (Same CDS sequence)	XM_017023217.1
Accession Version	XM_017023217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	lysine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATT CCAGAAATTA TAAATCAGAA GAAGAATTTA TTCATATTAA TAACAAACTG 
CGTCGGGGAG ACATAATTGG AGTTCAGGGG AATCCTGGTA AAACCAAGAA GGGTGAGCTG 
AGCATCATTC CGTATGAGAT CACACTGCTG TCTCCCTGTT TGCATATGTT ACCTCATCTT 
CACTTTGGCC TCAAAGACAA GGAAACAAGG TATCGCCAGA GATACTTGGA CTTGATCCTG 
AATGACTTTG TGAGGCAGAA ATTTATCATC CGCTCTAAGA TCATCACATA TATAAGAAGT 
TTCTTAGATG AGCTGGGATT CCTAGAGATT GAAACTCCCA TGATGAACAT CATCCCAGGG 
GGAGCCGTGG CCAAGCCTTT CATCACTTAT CACAACGAGC TGGACATGAA CTTATATATG 
AGAATTGCTC CAGAACTCTA TCATAAGATG CTTGTGGTTG GTGGCATCGA CCGGGTTTAT 
GAAATTGGAC GCCAGTTCCG GAATGAGGGG ATTGATTTGA CGCACAATCC TGAGTTCACC 
ACCTGTGAGT TCTACATGGC CTATGCAGAC TATCACGATC TCATGGAAAT CACGGAGAAG 
ATGGTTTCAG GGATGGTGAA GCATATTACA GGCAGTTACA AGGTCACCTA CCACCCAGAT 
GGCCCAGAGG GCCAAGCCTA CGATGTTGAC TTCACCCCAC CCTTCCGGCG AATCAACATG 
GTAGAAGAGC TTGAGAAAGC CCTGGGGATG AAGCTGCCAG AAACGAACCT CTTTGAAACT 
GAAGAAACTC GCAAAATTCT TGATGATATC TGTGTGGCAA AAGCTGTTGA ATGCCCTCCA 
CCTCGGACCA CAGCCAGGCT CCTTGACAAG CTTGTTGGGG AGTTCCTGGA AGTGACTTGC 
ATCAATCCTA CATTCATCTG TGATCACCCA CAGATAATGA GCCCTTTGGC TAAATGGCAC 
CGCTCTAAAG AGGGTCTGAC TGAGCGCTTT GAGCTGTTTG TCATGAAGAA AGAGATATGC 
AATGCGTATA CTGAGCTGAA TGATCCCATG CGGCAGCGGC AGCTTTTTGA AGAACAGGCC 
AAGGCCAAGG CTGCAGGTGA TGATGAGGCC ATGTTCATAG ATGAAAACTT CTGTACTGCC 
CTGGAATATG GGCTGCCCCC CACAGCTGGC TGGGGCATGG GCATTGATCG AGTCGCCATG 
TTTCTCACGG ACTCCAACAA CATCAAGGAA GTACTTCTGT TTCCTGCCAT GAAACCCGAA 
GACAAGAAGG AGAATGTAGC AACCACTGAT ACACTGGAAA GCACAACAGT TGGCACTTCT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878706.1
CDS	609..1934
Translation	MANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

Target ORF information:

RefSeq Version	XM_017023217.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023217.1

ORF Insert Sequence:

ATGGCCAATT CCAGAAATTA TAAATCAGAA GAAGAATTTA TTCATATTAA TAACAAACTG 
CGTCGGGGAG ACATAATTGG AGTTCAGGGG AATCCTGGTA AAACCAAGAA GGGTGAGCTG 
AGCATCATTC CGTATGAGAT CACACTGCTG TCTCCCTGTT TGCATATGTT ACCTCATCTT 
CACTTTGGCC TCAAAGACAA GGAAACAAGG TATCGCCAGA GATACTTGGA CTTGATCCTG 
AATGACTTTG TGAGGCAGAA ATTTATCATC CGCTCTAAGA TCATCACATA TATAAGAAGT 
TTCTTAGATG AGCTGGGATT CCTAGAGATT GAAACTCCCA TGATGAACAT CATCCCAGGG 
GGAGCCGTGG CCAAGCCTTT CATCACTTAT CACAACGAGC TGGACATGAA CTTATATATG 
AGAATTGCTC CAGAACTCTA TCATAAGATG CTTGTGGTTG GTGGCATCGA CCGGGTTTAT 
GAAATTGGAC GCCAGTTCCG GAATGAGGGG ATTGATTTGA CGCACAATCC TGAGTTCACC 
ACCTGTGAGT TCTACATGGC CTATGCAGAC TATCACGATC TCATGGAAAT CACGGAGAAG 
ATGGTTTCAG GGATGGTGAA GCATATTACA GGCAGTTACA AGGTCACCTA CCACCCAGAT 
GGCCCAGAGG GCCAAGCCTA CGATGTTGAC TTCACCCCAC CCTTCCGGCG AATCAACATG 
GTAGAAGAGC TTGAGAAAGC CCTGGGGATG AAGCTGCCAG AAACGAACCT CTTTGAAACT 
GAAGAAACTC GCAAAATTCT TGATGATATC TGTGTGGCAA AAGCTGTTGA ATGCCCTCCA 
CCTCGGACCA CAGCCAGGCT CCTTGACAAG CTTGTTGGGG AGTTCCTGGA AGTGACTTGC 
ATCAATCCTA CATTCATCTG TGATCACCCA CAGATAATGA GCCCTTTGGC TAAATGGCAC 
CGCTCTAAAG AGGGTCTGAC TGAGCGCTTT GAGCTGTTTG TCATGAAGAA AGAGATATGC 
AATGCGTATA CTGAGCTGAA TGATCCCATG CGGCAGCGGC AGCTTTTTGA AGAACAGGCC 
AAGGCCAAGG CTGCAGGTGA TGATGAGGCC ATGTTCATAG ATGAAAACTT CTGTACTGCC 
CTGGAATATG GGCTGCCCCC CACAGCTGGC TGGGGCATGG GCATTGATCG AGTCGCCATG 
TTTCTCACGG ACTCCAACAA CATCAAGGAA GTACTTCTGT TTCCTGCCAT GAAACCCGAA 
GACAAGAAGG AGAATGTAGC AACCACTGAT ACACTGGAAA GCACAACAGT TGGCACTTCT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KARS1 ( NM_005548.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005539.1 Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05050
Clone ID Related Accession (Same CDS sequence)	NM_005548.2
Accession Version	NM_005548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Homo sapiens(human)
Product	lysine--tRNA ligase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315687.1, AF285758.1 and AA503390.1. On Jul 16, 2008 this sequence version replaced NM_005548.1. Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an upstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.429235.1, SRR1803612.179789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG TGCAGGCGGC CGAGGTGAAA GTGGATGGCA GCGAGCCGAA ACTGAGCAAG 
AATGAGCTGA AGAGACGCCT GAAAGCTGAG AAGAAAGTAG CAGAGAAGGA GGCCAAACAG 
AAAGAGCTCA GTGAGAAACA GCTAAGCCAA GCCACTGCTG CTGCCACCAA CCACACCACT 
GATAATGGTG TGGGTCCTGA GGAAGAGAGC GTGGACCCAA ATCAATACTA CAAAATCCGC 
AGTCAAGCAA TTCATCAGCT GAAGGTCAAT GGGGAAGACC CATACCCACA CAAGTTCCAT 
GTAGACATCT CACTCACTGA CTTCATCCAA AAATATAGTC ACCTGCAGCC TGGGGATCAC 
CTGACTGACA TCACCTTAAA GGTGGCAGGT AGGATCCATG CCAAAAGAGC TTCTGGGGGA 
AAGCTCATCT TCTATGATCT TCGAGGAGAG GGGGTGAAGT TGCAAGTCAT GGCCAATTCC 
AGAAATTATA AATCAGAAGA AGAATTTATT CATATTAATA ACAAACTGCG TCGGGGAGAC 
ATAATTGGAG TTCAGGGGAA TCCTGGTAAA ACCAAGAAGG GTGAGCTGAG CATCATTCCG 
TATGAGATCA CACTGCTGTC TCCCTGTTTG CATATGTTAC CTCATCTTCA CTTTGGCCTC 
AAAGACAAGG AAACAAGGTA TCGCCAGAGA TACTTGGACT TGATCCTGAA TGACTTTGTG 
AGGCAGAAAT TTATCATCCG CTCTAAGATC ATCACATATA TAAGAAGTTT CTTAGATGAG 
CTGGGATTCC TAGAGATTGA AACTCCCATG ATGAACATCA TCCCAGGGGG AGCCGTGGCC 
AAGCCTTTCA TCACTTATCA CAACGAGCTG GACATGAACT TATATATGAG AATTGCTCCA 
GAACTCTATC ATAAGATGCT TGTGGTTGGT GGCATCGACC GGGTTTATGA AATTGGACGC 
CAGTTCCGGA ATGAGGGGAT TGATTTGACG CACAATCCTG AGTTCACCAC CTGTGAGTTC 
TACATGGCCT ATGCAGACTA TCACGATCTC ATGGAAATCA CGGAGAAGAT GGTTTCAGGG 
ATGGTGAAGC ATATTACAGG CAGTTACAAG GTCACCTACC ACCCAGATGG CCCAGAGGGC 
CAAGCCTACG ATGTTGACTT CACCCCACCC TTCCGGCGAA TCAACATGGT AGAAGAGCTT 
GAGAAAGCCC TGGGGATGAA GCTGCCAGAA ACGAACCTCT TTGAAACTGA AGAAACTCGC 
AAAATTCTTG ATGATATCTG TGTGGCAAAA GCTGTTGAAT GCCCTCCACC TCGGACCACA 
GCCAGGCTCC TTGACAAGCT TGTTGGGGAG TTCCTGGAAG TGACTTGCAT CAATCCTACA 
TTCATCTGTG ATCACCCACA GATAATGAGC CCTTTGGCTA AATGGCACCG CTCTAAAGAG 
GGTCTGACTG AGCGCTTTGA GCTGTTTGTC ATGAAGAAAG AGATATGCAA TGCGTATACT 
GAGCTGAATG ATCCCATGCG GCAGCGGCAG CTTTTTGAAG AACAGGCCAA GGCCAAGGCT 
GCAGGTGATG ATGAGGCCAT GTTCATAGAT GAAAACTTCT GTACTGCCCT GGAATATGGG 
CTGCCCCCCA CAGCTGGCTG GGGCATGGGC ATTGATCGAG TCGCCATGTT TCTCACGGAC 
TCCAACAACA TCAAGGAAGT ACTTCTGTTT CCTGCCATGA AACCCGAAGA CAAGAAGGAG 
AATGTAGCAA CCACTGATAC ACTGGAAAGC ACAACAGTTG GCACTTCTGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005539.1
CDS	49..1842
Translation	MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

Target ORF information:

RefSeq Version	NM_005548.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005548.2

ORF Insert Sequence:

ATGGCGGCCG TGCAGGCGGC CGAGGTGAAA GTGGATGGCA GCGAGCCGAA ACTGAGCAAG 
AATGAGCTGA AGAGACGCCT GAAAGCTGAG AAGAAAGTAG CAGAGAAGGA GGCCAAACAG 
AAAGAGCTCA GTGAGAAACA GCTAAGCCAA GCCACTGCTG CTGCCACCAA CCACACCACT 
GATAATGGTG TGGGTCCTGA GGAAGAGAGC GTGGACCCAA ATCAATACTA CAAAATCCGC 
AGTCAAGCAA TTCATCAGCT GAAGGTCAAT GGGGAAGACC CATACCCACA CAAGTTCCAT 
GTAGACATCT CACTCACTGA CTTCATCCAA AAATATAGTC ACCTGCAGCC TGGGGATCAC 
CTGACTGACA TCACCTTAAA GGTGGCAGGT AGGATCCATG CCAAAAGAGC TTCTGGGGGA 
AAGCTCATCT TCTATGATCT TCGAGGAGAG GGGGTGAAGT TGCAAGTCAT GGCCAATTCC 
AGAAATTATA AATCAGAAGA AGAATTTATT CATATTAATA ACAAACTGCG TCGGGGAGAC 
ATAATTGGAG TTCAGGGGAA TCCTGGTAAA ACCAAGAAGG GTGAGCTGAG CATCATTCCG 
TATGAGATCA CACTGCTGTC TCCCTGTTTG CATATGTTAC CTCATCTTCA CTTTGGCCTC 
AAAGACAAGG AAACAAGGTA TCGCCAGAGA TACTTGGACT TGATCCTGAA TGACTTTGTG 
AGGCAGAAAT TTATCATCCG CTCTAAGATC ATCACATATA TAAGAAGTTT CTTAGATGAG 
CTGGGATTCC TAGAGATTGA AACTCCCATG ATGAACATCA TCCCAGGGGG AGCCGTGGCC 
AAGCCTTTCA TCACTTATCA CAACGAGCTG GACATGAACT TATATATGAG AATTGCTCCA 
GAACTCTATC ATAAGATGCT TGTGGTTGGT GGCATCGACC GGGTTTATGA AATTGGACGC 
CAGTTCCGGA ATGAGGGGAT TGATTTGACG CACAATCCTG AGTTCACCAC CTGTGAGTTC 
TACATGGCCT ATGCAGACTA TCACGATCTC ATGGAAATCA CGGAGAAGAT GGTTTCAGGG 
ATGGTGAAGC ATATTACAGG CAGTTACAAG GTCACCTACC ACCCAGATGG CCCAGAGGGC 
CAAGCCTACG ATGTTGACTT CACCCCACCC TTCCGGCGAA TCAACATGGT AGAAGAGCTT 
GAGAAAGCCC TGGGGATGAA GCTGCCAGAA ACGAACCTCT TTGAAACTGA AGAAACTCGC 
AAAATTCTTG ATGATATCTG TGTGGCAAAA GCTGTTGAAT GCCCTCCACC TCGGACCACA 
GCCAGGCTCC TTGACAAGCT TGTTGGGGAG TTCCTGGAAG TGACTTGCAT CAATCCTACA 
TTCATCTGTG ATCACCCACA GATAATGAGC CCTTTGGCTA AATGGCACCG CTCTAAAGAG 
GGTCTGACTG AGCGCTTTGA GCTGTTTGTC ATGAAGAAAG AGATATGCAA TGCGTATACT 
GAGCTGAATG ATCCCATGCG GCAGCGGCAG CTTTTTGAAG AACAGGCCAA GGCCAAGGCT 
GCAGGTGATG ATGAGGCCAT GTTCATAGAT GAAAACTTCT GTACTGCCCT GGAATATGGG 
CTGCCCCCCA CAGCTGGCTG GGGCATGGGC ATTGATCGAG TCGCCATGTT TCTCACGGAC 
TCCAACAACA TCAAGGAAGT ACTTCTGTTT CCTGCCATGA AACCCGAAGA CAAGAAGGAG 
AATGTAGCAA CCACTGATAC ACTGGAAAGC ACAACAGTTG GCACTTCTGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KARS1 ( NM_001130089.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123561.1 Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18770
Clone ID Related Accession (Same CDS sequence)	NM_001130089.1
Accession Version	NM_001130089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	lysine--tRNA ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315687.1, AF285758.1, BM781594.1 and AA503390.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.126450.1, SRR1660809.13712.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGACGC AAGCTGCTGT AAGGCTTGTT AGGGGGTCCC TGCGCAAAAC CTCCTGGGCA 
GAGTGGGGTC ACAGGGAACT GCGACTGGGT CAACTTGCTC CTTTCACAGC GCCTCACAAG 
GACAAGTCAT TTTCTGATCA AAGAAGTGAG CTGAAGAGAC GCCTGAAAGC TGAGAAGAAA 
GTAGCAGAGA AGGAGGCCAA ACAGAAAGAG CTCAGTGAGA AACAGCTAAG CCAAGCCACT 
GCTGCTGCCA CCAACCACAC CACTGATAAT GGTGTGGGTC CTGAGGAAGA GAGCGTGGAC 
CCAAATCAAT ACTACAAAAT CCGCAGTCAA GCAATTCATC AGCTGAAGGT CAATGGGGAA 
GACCCATACC CACACAAGTT CCATGTAGAC ATCTCACTCA CTGACTTCAT CCAAAAATAT 
AGTCACCTGC AGCCTGGGGA TCACCTGACT GACATCACCT TAAAGGTGGC AGGTAGGATC 
CATGCCAAAA GAGCTTCTGG GGGAAAGCTC ATCTTCTATG ATCTTCGAGG AGAGGGGGTG 
AAGTTGCAAG TCATGGCCAA TTCCAGAAAT TATAAATCAG AAGAAGAATT TATTCATATT 
AATAACAAAC TGCGTCGGGG AGACATAATT GGAGTTCAGG GGAATCCTGG TAAAACCAAG 
AAGGGTGAGC TGAGCATCAT TCCGTATGAG ATCACACTGC TGTCTCCCTG TTTGCATATG 
TTACCTCATC TTCACTTTGG CCTCAAAGAC AAGGAAACAA GGTATCGCCA GAGATACTTG 
GACTTGATCC TGAATGACTT TGTGAGGCAG AAATTTATCA TCCGCTCTAA GATCATCACA 
TATATAAGAA GTTTCTTAGA TGAGCTGGGA TTCCTAGAGA TTGAAACTCC CATGATGAAC 
ATCATCCCAG GGGGAGCCGT GGCCAAGCCT TTCATCACTT ATCACAACGA GCTGGACATG 
AACTTATATA TGAGAATTGC TCCAGAACTC TATCATAAGA TGCTTGTGGT TGGTGGCATC 
GACCGGGTTT ATGAAATTGG ACGCCAGTTC CGGAATGAGG GGATTGATTT GACGCACAAT 
CCTGAGTTCA CCACCTGTGA GTTCTACATG GCCTATGCAG ACTATCACGA TCTCATGGAA 
ATCACGGAGA AGATGGTTTC AGGGATGGTG AAGCATATTA CAGGCAGTTA CAAGGTCACC 
TACCACCCAG ATGGCCCAGA GGGCCAAGCC TACGATGTTG ACTTCACCCC ACCCTTCCGG 
CGAATCAACA TGGTAGAAGA GCTTGAGAAA GCCCTGGGGA TGAAGCTGCC AGAAACGAAC 
CTCTTTGAAA CTGAAGAAAC TCGCAAAATT CTTGATGATA TCTGTGTGGC AAAAGCTGTT 
GAATGCCCTC CACCTCGGAC CACAGCCAGG CTCCTTGACA AGCTTGTTGG GGAGTTCCTG 
GAAGTGACTT GCATCAATCC TACATTCATC TGTGATCACC CACAGATAAT GAGCCCTTTG 
GCTAAATGGC ACCGCTCTAA AGAGGGTCTG ACTGAGCGCT TTGAGCTGTT TGTCATGAAG 
AAAGAGATAT GCAATGCGTA TACTGAGCTG AATGATCCCA TGCGGCAGCG GCAGCTTTTT 
GAAGAACAGG CCAAGGCCAA GGCTGCAGGT GATGATGAGG CCATGTTCAT AGATGAAAAC 
TTCTGTACTG CCCTGGAATA TGGGCTGCCC CCCACAGCTG GCTGGGGCAT GGGCATTGAT 
CGAGTCGCCA TGTTTCTCAC GGACTCCAAC AACATCAAGG AAGTACTTCT GTTTCCTGCC 
ATGAAACCCG AAGACAAGAA GGAGAATGTA GCAACCACTG ATACACTGGA AAGCACAACA 
GTTGGCACTT CTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123561.1
CDS	145..2022
Translation	MLTQAAVRLVRGSLRKTSWAEWGHRELRLGQLAPFTAPHKDKSFSDQRSELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

Target ORF information:

RefSeq Version	NM_001130089.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130089.1

ORF Insert Sequence:

ATGTTGACGC AAGCTGCTGT AAGGCTTGTT AGGGGGTCCC TGCGCAAAAC CTCCTGGGCA 
GAGTGGGGTC ACAGGGAACT GCGACTGGGT CAACTTGCTC CTTTCACAGC GCCTCACAAG 
GACAAGTCAT TTTCTGATCA AAGAAGTGAG CTGAAGAGAC GCCTGAAAGC TGAGAAGAAA 
GTAGCAGAGA AGGAGGCCAA ACAGAAAGAG CTCAGTGAGA AACAGCTAAG CCAAGCCACT 
GCTGCTGCCA CCAACCACAC CACTGATAAT GGTGTGGGTC CTGAGGAAGA GAGCGTGGAC 
CCAAATCAAT ACTACAAAAT CCGCAGTCAA GCAATTCATC AGCTGAAGGT CAATGGGGAA 
GACCCATACC CACACAAGTT CCATGTAGAC ATCTCACTCA CTGACTTCAT CCAAAAATAT 
AGTCACCTGC AGCCTGGGGA TCACCTGACT GACATCACCT TAAAGGTGGC AGGTAGGATC 
CATGCCAAAA GAGCTTCTGG GGGAAAGCTC ATCTTCTATG ATCTTCGAGG AGAGGGGGTG 
AAGTTGCAAG TCATGGCCAA TTCCAGAAAT TATAAATCAG AAGAAGAATT TATTCATATT 
AATAACAAAC TGCGTCGGGG AGACATAATT GGAGTTCAGG GGAATCCTGG TAAAACCAAG 
AAGGGTGAGC TGAGCATCAT TCCGTATGAG ATCACACTGC TGTCTCCCTG TTTGCATATG 
TTACCTCATC TTCACTTTGG CCTCAAAGAC AAGGAAACAA GGTATCGCCA GAGATACTTG 
GACTTGATCC TGAATGACTT TGTGAGGCAG AAATTTATCA TCCGCTCTAA GATCATCACA 
TATATAAGAA GTTTCTTAGA TGAGCTGGGA TTCCTAGAGA TTGAAACTCC CATGATGAAC 
ATCATCCCAG GGGGAGCCGT GGCCAAGCCT TTCATCACTT ATCACAACGA GCTGGACATG 
AACTTATATA TGAGAATTGC TCCAGAACTC TATCATAAGA TGCTTGTGGT TGGTGGCATC 
GACCGGGTTT ATGAAATTGG ACGCCAGTTC CGGAATGAGG GGATTGATTT GACGCACAAT 
CCTGAGTTCA CCACCTGTGA GTTCTACATG GCCTATGCAG ACTATCACGA TCTCATGGAA 
ATCACGGAGA AGATGGTTTC AGGGATGGTG AAGCATATTA CAGGCAGTTA CAAGGTCACC 
TACCACCCAG ATGGCCCAGA GGGCCAAGCC TACGATGTTG ACTTCACCCC ACCCTTCCGG 
CGAATCAACA TGGTAGAAGA GCTTGAGAAA GCCCTGGGGA TGAAGCTGCC AGAAACGAAC 
CTCTTTGAAA CTGAAGAAAC TCGCAAAATT CTTGATGATA TCTGTGTGGC AAAAGCTGTT 
GAATGCCCTC CACCTCGGAC CACAGCCAGG CTCCTTGACA AGCTTGTTGG GGAGTTCCTG 
GAAGTGACTT GCATCAATCC TACATTCATC TGTGATCACC CACAGATAAT GAGCCCTTTG 
GCTAAATGGC ACCGCTCTAA AGAGGGTCTG ACTGAGCGCT TTGAGCTGTT TGTCATGAAG 
AAAGAGATAT GCAATGCGTA TACTGAGCTG AATGATCCCA TGCGGCAGCG GCAGCTTTTT 
GAAGAACAGG CCAAGGCCAA GGCTGCAGGT GATGATGAGG CCATGTTCAT AGATGAAAAC 
TTCTGTACTG CCCTGGAATA TGGGCTGCCC CCCACAGCTG GCTGGGGCAT GGGCATTGAT 
CGAGTCGCCA TGTTTCTCAC GGACTCCAAC AACATCAAGG AAGTACTTCT GTTTCCTGCC 
ATGAAACCCG AAGACAAGAA GGAGAATGTA GCAACCACTG ATACACTGGA AAGCACAACA 
GTTGGCACTT CTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biallelic variants in
Neurology92(11)e1225-e1237(2019 03)
van der Knaap MS,Bugiani M,Mendes MI,Riley LG,Smith DEC,Rudinger-Thirion J,Frugier M,Breur M,Crawford J,van Gaalen J,Schouten M,Willems M,Waisfisz Q,Mau-Them FT,Rodenburg RJ,Taft RJ,Keren B,Christodoulou J,Depienne C,Simons C,Salomons GS,Mochel F

Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis.
The Journal of clinical investigation128(11)5034-5055(2018 11)
Nam SH,Kim D,Lee D,Lee HM,Song DG,Jung JW,Kim JE,Kim HJ,Kwon NH,Jo EK,Kim S,Lee JW

Three human aminoacyl-tRNA synthetases have distinct sub-mitochondrial localizations that are unaffected by disease-associated mutations.
The Journal of biological chemistry293(35)13604-13615(2018 08)
González-Serrano LE,Karim L,Pierre F,Schwenzer H,Rötig A,Munnich A,Sissler M

LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Molecular systems biology14(7)e8071(2018 07)
Trepte P,Kruse S,Kostova S,Hoffmann S,Buntru A,Tempelmeier A,Secker C,Diez L,Schulz A,Klockmeier K,Zenkner M,Golusik S,Rau K,Schnoegl S,Garner CC,Wanker EE

Mutations in KARS cause early-onset hearing loss and leukoencephalopathy: Potential pathogenic mechanism.
Human mutation38(12)1740-1750(2017 12)
Zhou XL,He LX,Yu LJ,Wang Y,Wang XJ,Wang ED,Yang T

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KARS1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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