LysRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LysRS
Entrez Gene ID	31904
Full Name	Lysyl-tRNA synthetase
Synonyms	Aats-lys,CG12141,Dmel\CG12141,KRS
General protein information	Preferred Names Lysyl-tRNA synthetase Names CG12141 gene product from transcript CG12141-RA CG12141-PA CG12141-PB CG12141-PC LysRS-PA LysRS-PB LysRS-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8F1-8F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167196.3	NP_727353.1	Lysyl-tRNA synthetase, isoform B
NM_001201642.2	NP_001188571.1	Lysyl-tRNA synthetase, isoform C
NM_132345.2	NP_572573.1	Lysyl-tRNA synthetase, isoform A

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	krs1	NP_595892.1
Macaca mulatta (Rhesus monkey)	KARS	XP_001104312.2
Neurospora crassa	NCU04020	XP_957635.1
Canis lupus familiaris (dog)	KARS	XP_861533.1
Saccharomyces cerevisiae (baker's yeast)	KRS1	NP_010322.1
Xenopus tropicalis (tropical clawed frog)	kars	NP_001001255.1
Oryza sativa (rice)	Os03g0586800	NP_001050572.1
Homo sapiens (human)	KARS	NP_001123561.1
Mus musculus (house mouse)	Kars	NP_001124340.1
Danio rerio (zebrafish)	kars	NP_001002386.1
Caenorhabditis elegans (roundworm)	kars-1	NP_495453.1
Kluyveromyces lactis	KLLA0F18392g	XP_455904.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007858	XP_317634.3
Eremothecium gossypii	AGOS_AGR037C	NP_986702.1
Bos taurus (cattle)	KARS	NP_001029528.1
Rattus norvegicus (Norway rat)	Kars	NP_001006968.1
Arabidopsis thaliana (thale cress)	ATKRS-1	NP_187777.1
Magnaporthe oryzae (rice blast fungus)	MGG_04966	XP_003712462.1
Pan troglodytes (chimpanzee)	KARS	XP_003315273.1
Gallus gallus (chicken)	KARS	NP_001025754.1
Drosophila melanogaster (fruit fly)	Aats-lys	NP_727353.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000325	XP_310792.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LysRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LysRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01179	NM_167196.3 Latest version!	Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa01179	NM_001201642.2 Latest version!	Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa03359	NM_132345.2 Latest version!	Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LysRS ( NM_167196.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727353.1 Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01179
Clone ID Related Accession (Same CDS sequence)	NM_167196.3 , NM_001201642.2
Accession Version	NM_167196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Lysyl-tRNA synthetase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167196.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGCCC GCTGCGCCCT TAGAACCGTG CAGGGGTCAT GGCCCCGATG CAGATACACC 
GGAATTCAGC CACTTGCCGC CGGTTTCCAC GTGAGCGCTC CGCAAATGGA GCCAAGCAAG 
CAGACCAAAC TTGAGCTAAA GCGTCGCCTG AAGGCCGAGC AAAAGGCCAA GGAGAAGGCT 
GAGAAGGCGG CCGCTGCGCC GGCGGAAAAT ACTGCTGGCA AGAAGAAGAG CAGTGCCGCC 
GAGGAGGAGG AGATCTCGCC GAATGAATAC TTTAAGCTGC GCTCCGCCGC CGTTCAGGAG 
CTGAAACGTT CACCCGCAAC GGATCCCTAT CCACACAAGT TCCATGTGAG TAGCTCGCTG 
GAAGACTTCA TTGCCAAGTA CGAGAACAGC CTCAAGGAGG GCGAGACCCT GGAGAATGTC 
AAACTGAGCG TGGCTGGGCG CGTGCATGCC ATACGCGAGT CGGGCGCCAA GCTCATTTTC 
TACGATCTGC GCGGCGAGGG CGTCAAGGTG CAGGTGATGG CCAGCGCTAA GTCCTACAAG 
TCGGAGGCGG ATTTCGAAAT CGATACGTCC AAATTGCGAC GTGGTGATAT CATCGGCGTT 
GTCGGACATC CCGGCAAAAC CAAGAAGGGT GAACTGTCCG TTATGCCCAG CGAGATCAAG 
CTGCTATCGC CCTGTCTACA CATGCTGCCC CATCTGCATT TCGGGTTGAA GGACAAGGAG 
ACGCGCTACC GCCAGCGCTA TCTTGATCTC ATTCTCAACA ACAATGTGCG AGAGAAATTC 
CAGATCCGTG CCAAGATCAT CTCGTACGTG CGTCAGTTCC TGGATCGTCT GGGTTTCCTG 
GAGATCGAGA CGCCCATGAT GAACATGATC GCCGGTGGAG CCACTGCCAA GCCCTTCGTG 
ACGCATCACA ATGACCTCAA GATGGATCTG TTCATGCGCA TTGCTCCGGA GCTGTATCAC 
AAGATGCTGG TGGTGGGTGG ACTCGATCGT GTGTACGAGA TCGGACGGCA GTTCCGCAAC 
GAGGGCATCG ACCTCACCCA CAATCCTGAA TTCACCACGT GCGAGTTCTA CATGGCGTAT 
GCCGACTACG CTGACATTAT GGATATCACC GAGCAGTTGG TCTCGGGAAT GGTGAAAGCG 
ATCCGTGGCT CCTACAAGGT CATCTATCAT CCGGAGGGTC CAGAGGGACC TGAGCAAGAA 
CTTGACTTTA CGCCGCCCTT TAAGCGCGTA TCCATGATCA AAACGCTGGA AGAGAAGCTG 
CAGGTGAAGT TCCCCGCGGC CGACACCTTC AATTCGCCAG AGACGAATCA GTTCCTGTCT 
CAGCTGTGCG CTAAGCACCA GGTCGAGTGC CCAGCTCCGC GCACCACTGC CCGCCTGCTG 
GACAAGCTCG TCGGCGAATT CATCGAGGAG TTTTGCGTGA ATCCAACGTT CATCTGTGAG 
CATCCGCAGA TCATGTCGCC GCTGGCCAAG TACCATCGCA GTATTCCCGG CCTGACGGAG 
CGCTTCGAAC TGTTTGTGGC CAAGAAGGAG ATTTGCAACG CGTATACCGA GTTGAACGAT 
CCGGTTGTGC AGCGCGAGCG TTTCGAGCAG CAGGCCAGCG ACAAGGCGGC CGGCGATGAT 
GAGGCCCAGC TGGTCGACGA GAACTTCTGC ACGTCCCTGG AGTACGGACT GCCGCCCACC 
GGTGGTTTTG GCATGGGCAT CGATCGCCTG GCCATGTTCC TCACGGACTC CAACAACATC 
AAGGAGGTTC TGCTGTTCCC CGCCATGAAA CCGGAGGATG CCAACCGCAC GGCAGCGGAA 
ACTGCTCCCA CCGCACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727353.1
CDS	290..2113
Translation	MFARCALRTVQGSWPRCRYTGIQPLAAGFHVSAPQMEPSKQTKLELKRRLKAEQKAKEKAEKAAAAPAENTAGKKKSSAAEEEEISPNEYFKLRSAAVQELKRSPATDPYPHKFHVSSSLEDFIAKYENSLKEGETLENVKLSVAGRVHAIRESGAKLIFYDLRGEGVKVQVMASAKSYKSEADFEIDTSKLRRGDIIGVVGHPGKTKKGELSVMPSEIKLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNNNVREKFQIRAKIISYVRQFLDRLGFLEIETPMMNMIAGGATAKPFVTHHNDLKMDLFMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYADIMDITEQLVSGMVKAIRGSYKVIYHPEGPEGPEQELDFTPPFKRVSMIKTLEEKLQVKFPAADTFNSPETNQFLSQLCAKHQVECPAPRTTARLLDKLVGEFIEEFCVNPTFICEHPQIMSPLAKYHRSIPGLTERFELFVAKKEICNAYTELNDPVVQRERFEQQASDKAAGDDEAQLVDENFCTSLEYGLPPTGGFGMGIDRLAMFLTDSNNIKEVLLFPAMKPEDANRTAAETAPTAQQ

Target ORF information:

RefSeq Version	NM_167196.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167196.3

ORF Insert Sequence:

ATGTTCGCCC GCTGCGCCCT TAGAACCGTG CAGGGGTCAT GGCCCCGATG CAGATACACC 
GGAATTCAGC CACTTGCCGC CGGTTTCCAC GTGAGCGCTC CGCAAATGGA GCCAAGCAAG 
CAGACCAAAC TTGAGCTAAA GCGTCGCCTG AAGGCCGAGC AAAAGGCCAA GGAGAAGGCT 
GAGAAGGCGG CCGCTGCGCC GGCGGAAAAT ACTGCTGGCA AGAAGAAGAG CAGTGCCGCC 
GAGGAGGAGG AGATCTCGCC GAATGAATAC TTTAAGCTGC GCTCCGCCGC CGTTCAGGAG 
CTGAAACGTT CACCCGCAAC GGATCCCTAT CCACACAAGT TCCATGTGAG TAGCTCGCTG 
GAAGACTTCA TTGCCAAGTA CGAGAACAGC CTCAAGGAGG GCGAGACCCT GGAGAATGTC 
AAACTGAGCG TGGCTGGGCG CGTGCATGCC ATACGCGAGT CGGGCGCCAA GCTCATTTTC 
TACGATCTGC GCGGCGAGGG CGTCAAGGTG CAGGTGATGG CCAGCGCTAA GTCCTACAAG 
TCGGAGGCGG ATTTCGAAAT CGATACGTCC AAATTGCGAC GTGGTGATAT CATCGGCGTT 
GTCGGACATC CCGGCAAAAC CAAGAAGGGT GAACTGTCCG TTATGCCCAG CGAGATCAAG 
CTGCTATCGC CCTGTCTACA CATGCTGCCC CATCTGCATT TCGGGTTGAA GGACAAGGAG 
ACGCGCTACC GCCAGCGCTA TCTTGATCTC ATTCTCAACA ACAATGTGCG AGAGAAATTC 
CAGATCCGTG CCAAGATCAT CTCGTACGTG CGTCAGTTCC TGGATCGTCT GGGTTTCCTG 
GAGATCGAGA CGCCCATGAT GAACATGATC GCCGGTGGAG CCACTGCCAA GCCCTTCGTG 
ACGCATCACA ATGACCTCAA GATGGATCTG TTCATGCGCA TTGCTCCGGA GCTGTATCAC 
AAGATGCTGG TGGTGGGTGG ACTCGATCGT GTGTACGAGA TCGGACGGCA GTTCCGCAAC 
GAGGGCATCG ACCTCACCCA CAATCCTGAA TTCACCACGT GCGAGTTCTA CATGGCGTAT 
GCCGACTACG CTGACATTAT GGATATCACC GAGCAGTTGG TCTCGGGAAT GGTGAAAGCG 
ATCCGTGGCT CCTACAAGGT CATCTATCAT CCGGAGGGTC CAGAGGGACC TGAGCAAGAA 
CTTGACTTTA CGCCGCCCTT TAAGCGCGTA TCCATGATCA AAACGCTGGA AGAGAAGCTG 
CAGGTGAAGT TCCCCGCGGC CGACACCTTC AATTCGCCAG AGACGAATCA GTTCCTGTCT 
CAGCTGTGCG CTAAGCACCA GGTCGAGTGC CCAGCTCCGC GCACCACTGC CCGCCTGCTG 
GACAAGCTCG TCGGCGAATT CATCGAGGAG TTTTGCGTGA ATCCAACGTT CATCTGTGAG 
CATCCGCAGA TCATGTCGCC GCTGGCCAAG TACCATCGCA GTATTCCCGG CCTGACGGAG 
CGCTTCGAAC TGTTTGTGGC CAAGAAGGAG ATTTGCAACG CGTATACCGA GTTGAACGAT 
CCGGTTGTGC AGCGCGAGCG TTTCGAGCAG CAGGCCAGCG ACAAGGCGGC CGGCGATGAT 
GAGGCCCAGC TGGTCGACGA GAACTTCTGC ACGTCCCTGG AGTACGGACT GCCGCCCACC 
GGTGGTTTTG GCATGGGCAT CGATCGCCTG GCCATGTTCC TCACGGACTC CAACAACATC 
AAGGAGGTTC TGCTGTTCCC CGCCATGAAA CCGGAGGATG CCAACCGCAC GGCAGCGGAA 
ACTGCTCCCA CCGCACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LysRS ( NM_001201642.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188571.1 Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01179
Clone ID Related Accession (Same CDS sequence)	NM_167196.3 , NM_001201642.2
Accession Version	NM_001201642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Lysyl-tRNA synthetase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001201642.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGCCC GCTGCGCCCT TAGAACCGTG CAGGGGTCAT GGCCCCGATG CAGATACACC 
GGAATTCAGC CACTTGCCGC CGGTTTCCAC GTGAGCGCTC CGCAAATGGA GCCAAGCAAG 
CAGACCAAAC TTGAGCTAAA GCGTCGCCTG AAGGCCGAGC AAAAGGCCAA GGAGAAGGCT 
GAGAAGGCGG CCGCTGCGCC GGCGGAAAAT ACTGCTGGCA AGAAGAAGAG CAGTGCCGCC 
GAGGAGGAGG AGATCTCGCC GAATGAATAC TTTAAGCTGC GCTCCGCCGC CGTTCAGGAG 
CTGAAACGTT CACCCGCAAC GGATCCCTAT CCACACAAGT TCCATGTGAG TAGCTCGCTG 
GAAGACTTCA TTGCCAAGTA CGAGAACAGC CTCAAGGAGG GCGAGACCCT GGAGAATGTC 
AAACTGAGCG TGGCTGGGCG CGTGCATGCC ATACGCGAGT CGGGCGCCAA GCTCATTTTC 
TACGATCTGC GCGGCGAGGG CGTCAAGGTG CAGGTGATGG CCAGCGCTAA GTCCTACAAG 
TCGGAGGCGG ATTTCGAAAT CGATACGTCC AAATTGCGAC GTGGTGATAT CATCGGCGTT 
GTCGGACATC CCGGCAAAAC CAAGAAGGGT GAACTGTCCG TTATGCCCAG CGAGATCAAG 
CTGCTATCGC CCTGTCTACA CATGCTGCCC CATCTGCATT TCGGGTTGAA GGACAAGGAG 
ACGCGCTACC GCCAGCGCTA TCTTGATCTC ATTCTCAACA ACAATGTGCG AGAGAAATTC 
CAGATCCGTG CCAAGATCAT CTCGTACGTG CGTCAGTTCC TGGATCGTCT GGGTTTCCTG 
GAGATCGAGA CGCCCATGAT GAACATGATC GCCGGTGGAG CCACTGCCAA GCCCTTCGTG 
ACGCATCACA ATGACCTCAA GATGGATCTG TTCATGCGCA TTGCTCCGGA GCTGTATCAC 
AAGATGCTGG TGGTGGGTGG ACTCGATCGT GTGTACGAGA TCGGACGGCA GTTCCGCAAC 
GAGGGCATCG ACCTCACCCA CAATCCTGAA TTCACCACGT GCGAGTTCTA CATGGCGTAT 
GCCGACTACG CTGACATTAT GGATATCACC GAGCAGTTGG TCTCGGGAAT GGTGAAAGCG 
ATCCGTGGCT CCTACAAGGT CATCTATCAT CCGGAGGGTC CAGAGGGACC TGAGCAAGAA 
CTTGACTTTA CGCCGCCCTT TAAGCGCGTA TCCATGATCA AAACGCTGGA AGAGAAGCTG 
CAGGTGAAGT TCCCCGCGGC CGACACCTTC AATTCGCCAG AGACGAATCA GTTCCTGTCT 
CAGCTGTGCG CTAAGCACCA GGTCGAGTGC CCAGCTCCGC GCACCACTGC CCGCCTGCTG 
GACAAGCTCG TCGGCGAATT CATCGAGGAG TTTTGCGTGA ATCCAACGTT CATCTGTGAG 
CATCCGCAGA TCATGTCGCC GCTGGCCAAG TACCATCGCA GTATTCCCGG CCTGACGGAG 
CGCTTCGAAC TGTTTGTGGC CAAGAAGGAG ATTTGCAACG CGTATACCGA GTTGAACGAT 
CCGGTTGTGC AGCGCGAGCG TTTCGAGCAG CAGGCCAGCG ACAAGGCGGC CGGCGATGAT 
GAGGCCCAGC TGGTCGACGA GAACTTCTGC ACGTCCCTGG AGTACGGACT GCCGCCCACC 
GGTGGTTTTG GCATGGGCAT CGATCGCCTG GCCATGTTCC TCACGGACTC CAACAACATC 
AAGGAGGTTC TGCTGTTCCC CGCCATGAAA CCGGAGGATG CCAACCGCAC GGCAGCGGAA 
ACTGCTCCCA CCGCACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188571.1
CDS	227..2050
Translation	MFARCALRTVQGSWPRCRYTGIQPLAAGFHVSAPQMEPSKQTKLELKRRLKAEQKAKEKAEKAAAAPAENTAGKKKSSAAEEEEISPNEYFKLRSAAVQELKRSPATDPYPHKFHVSSSLEDFIAKYENSLKEGETLENVKLSVAGRVHAIRESGAKLIFYDLRGEGVKVQVMASAKSYKSEADFEIDTSKLRRGDIIGVVGHPGKTKKGELSVMPSEIKLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNNNVREKFQIRAKIISYVRQFLDRLGFLEIETPMMNMIAGGATAKPFVTHHNDLKMDLFMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYADIMDITEQLVSGMVKAIRGSYKVIYHPEGPEGPEQELDFTPPFKRVSMIKTLEEKLQVKFPAADTFNSPETNQFLSQLCAKHQVECPAPRTTARLLDKLVGEFIEEFCVNPTFICEHPQIMSPLAKYHRSIPGLTERFELFVAKKEICNAYTELNDPVVQRERFEQQASDKAAGDDEAQLVDENFCTSLEYGLPPTGGFGMGIDRLAMFLTDSNNIKEVLLFPAMKPEDANRTAAETAPTAQQ

Target ORF information:

RefSeq Version	NM_001201642.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201642.2

ORF Insert Sequence:

ATGTTCGCCC GCTGCGCCCT TAGAACCGTG CAGGGGTCAT GGCCCCGATG CAGATACACC 
GGAATTCAGC CACTTGCCGC CGGTTTCCAC GTGAGCGCTC CGCAAATGGA GCCAAGCAAG 
CAGACCAAAC TTGAGCTAAA GCGTCGCCTG AAGGCCGAGC AAAAGGCCAA GGAGAAGGCT 
GAGAAGGCGG CCGCTGCGCC GGCGGAAAAT ACTGCTGGCA AGAAGAAGAG CAGTGCCGCC 
GAGGAGGAGG AGATCTCGCC GAATGAATAC TTTAAGCTGC GCTCCGCCGC CGTTCAGGAG 
CTGAAACGTT CACCCGCAAC GGATCCCTAT CCACACAAGT TCCATGTGAG TAGCTCGCTG 
GAAGACTTCA TTGCCAAGTA CGAGAACAGC CTCAAGGAGG GCGAGACCCT GGAGAATGTC 
AAACTGAGCG TGGCTGGGCG CGTGCATGCC ATACGCGAGT CGGGCGCCAA GCTCATTTTC 
TACGATCTGC GCGGCGAGGG CGTCAAGGTG CAGGTGATGG CCAGCGCTAA GTCCTACAAG 
TCGGAGGCGG ATTTCGAAAT CGATACGTCC AAATTGCGAC GTGGTGATAT CATCGGCGTT 
GTCGGACATC CCGGCAAAAC CAAGAAGGGT GAACTGTCCG TTATGCCCAG CGAGATCAAG 
CTGCTATCGC CCTGTCTACA CATGCTGCCC CATCTGCATT TCGGGTTGAA GGACAAGGAG 
ACGCGCTACC GCCAGCGCTA TCTTGATCTC ATTCTCAACA ACAATGTGCG AGAGAAATTC 
CAGATCCGTG CCAAGATCAT CTCGTACGTG CGTCAGTTCC TGGATCGTCT GGGTTTCCTG 
GAGATCGAGA CGCCCATGAT GAACATGATC GCCGGTGGAG CCACTGCCAA GCCCTTCGTG 
ACGCATCACA ATGACCTCAA GATGGATCTG TTCATGCGCA TTGCTCCGGA GCTGTATCAC 
AAGATGCTGG TGGTGGGTGG ACTCGATCGT GTGTACGAGA TCGGACGGCA GTTCCGCAAC 
GAGGGCATCG ACCTCACCCA CAATCCTGAA TTCACCACGT GCGAGTTCTA CATGGCGTAT 
GCCGACTACG CTGACATTAT GGATATCACC GAGCAGTTGG TCTCGGGAAT GGTGAAAGCG 
ATCCGTGGCT CCTACAAGGT CATCTATCAT CCGGAGGGTC CAGAGGGACC TGAGCAAGAA 
CTTGACTTTA CGCCGCCCTT TAAGCGCGTA TCCATGATCA AAACGCTGGA AGAGAAGCTG 
CAGGTGAAGT TCCCCGCGGC CGACACCTTC AATTCGCCAG AGACGAATCA GTTCCTGTCT 
CAGCTGTGCG CTAAGCACCA GGTCGAGTGC CCAGCTCCGC GCACCACTGC CCGCCTGCTG 
GACAAGCTCG TCGGCGAATT CATCGAGGAG TTTTGCGTGA ATCCAACGTT CATCTGTGAG 
CATCCGCAGA TCATGTCGCC GCTGGCCAAG TACCATCGCA GTATTCCCGG CCTGACGGAG 
CGCTTCGAAC TGTTTGTGGC CAAGAAGGAG ATTTGCAACG CGTATACCGA GTTGAACGAT 
CCGGTTGTGC AGCGCGAGCG TTTCGAGCAG CAGGCCAGCG ACAAGGCGGC CGGCGATGAT 
GAGGCCCAGC TGGTCGACGA GAACTTCTGC ACGTCCCTGG AGTACGGACT GCCGCCCACC 
GGTGGTTTTG GCATGGGCAT CGATCGCCTG GCCATGTTCC TCACGGACTC CAACAACATC 
AAGGAGGTTC TGCTGTTCCC CGCCATGAAA CCGGAGGATG CCAACCGCAC GGCAGCGGAA 
ACTGCTCCCA CCGCACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LysRS ( NM_132345.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572573.1 Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03359
Clone ID Related Accession (Same CDS sequence)	NM_132345.2
Accession Version	NM_132345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Lysyl-tRNA synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_132345.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCTG CAAGTGGAGA ACTGTCCAAG AATGAGCTAA AGCGTCGCCT GAAGGCCGAG 
CAAAAGGCCA AGGAGAAGGC TGAGAAGGCG GCCGCTGCGC CGGCGGAAAA TACTGCTGGC 
AAGAAGAAGA GCAGTGCCGC CGAGGAGGAG GAGATCTCGC CGAATGAATA CTTTAAGCTG 
CGCTCCGCCG CCGTTCAGGA GCTGAAACGT TCACCCGCAA CGGATCCCTA TCCACACAAG 
TTCCATGTGA GTAGCTCGCT GGAAGACTTC ATTGCCAAGT ACGAGAACAG CCTCAAGGAG 
GGCGAGACCC TGGAGAATGT CAAACTGAGC GTGGCTGGGC GCGTGCATGC CATACGCGAG 
TCGGGCGCCA AGCTCATTTT CTACGATCTG CGCGGCGAGG GCGTCAAGGT GCAGGTGATG 
GCCAGCGCTA AGTCCTACAA GTCGGAGGCG GATTTCGAAA TCGATACGTC CAAATTGCGA 
CGTGGTGATA TCATCGGCGT TGTCGGACAT CCCGGCAAAA CCAAGAAGGG TGAACTGTCC 
GTTATGCCCA GCGAGATCAA GCTGCTATCG CCCTGTCTAC ACATGCTGCC CCATCTGCAT 
TTCGGGTTGA AGGACAAGGA GACGCGCTAC CGCCAGCGCT ATCTTGATCT CATTCTCAAC 
AACAATGTGC GAGAGAAATT CCAGATCCGT GCCAAGATCA TCTCGTACGT GCGTCAGTTC 
CTGGATCGTC TGGGTTTCCT GGAGATCGAG ACGCCCATGA TGAACATGAT CGCCGGTGGA 
GCCACTGCCA AGCCCTTCGT GACGCATCAC AATGACCTCA AGATGGATCT GTTCATGCGC 
ATTGCTCCGG AGCTGTATCA CAAGATGCTG GTGGTGGGTG GACTCGATCG TGTGTACGAG 
ATCGGACGGC AGTTCCGCAA CGAGGGCATC GACCTCACCC ACAATCCTGA ATTCACCACG 
TGCGAGTTCT ACATGGCGTA TGCCGACTAC GCTGACATTA TGGATATCAC CGAGCAGTTG 
GTCTCGGGAA TGGTGAAAGC GATCCGTGGC TCCTACAAGG TCATCTATCA TCCGGAGGGT 
CCAGAGGGAC CTGAGCAAGA ACTTGACTTT ACGCCGCCCT TTAAGCGCGT ATCCATGATC 
AAAACGCTGG AAGAGAAGCT GCAGGTGAAG TTCCCCGCGG CCGACACCTT CAATTCGCCA 
GAGACGAATC AGTTCCTGTC TCAGCTGTGC GCTAAGCACC AGGTCGAGTG CCCAGCTCCG 
CGCACCACTG CCCGCCTGCT GGACAAGCTC GTCGGCGAAT TCATCGAGGA GTTTTGCGTG 
AATCCAACGT TCATCTGTGA GCATCCGCAG ATCATGTCGC CGCTGGCCAA GTACCATCGC 
AGTATTCCCG GCCTGACGGA GCGCTTCGAA CTGTTTGTGG CCAAGAAGGA GATTTGCAAC 
GCGTATACCG AGTTGAACGA TCCGGTTGTG CAGCGCGAGC GTTTCGAGCA GCAGGCCAGC 
GACAAGGCGG CCGGCGATGA TGAGGCCCAG CTGGTCGACG AGAACTTCTG CACGTCCCTG 
GAGTACGGAC TGCCGCCCAC CGGTGGTTTT GGCATGGGCA TCGATCGCCT GGCCATGTTC 
CTCACGGACT CCAACAACAT CAAGGAGGTT CTGCTGTTCC CCGCCATGAA ACCGGAGGAT 
GCCAACCGCA CGGCAGCGGA AACTGCTCCC ACCGCACAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572573.1
CDS	135..1859
Translation	MAAASGELSKNELKRRLKAEQKAKEKAEKAAAAPAENTAGKKKSSAAEEEEISPNEYFKLRSAAVQELKRSPATDPYPHKFHVSSSLEDFIAKYENSLKEGETLENVKLSVAGRVHAIRESGAKLIFYDLRGEGVKVQVMASAKSYKSEADFEIDTSKLRRGDIIGVVGHPGKTKKGELSVMPSEIKLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNNNVREKFQIRAKIISYVRQFLDRLGFLEIETPMMNMIAGGATAKPFVTHHNDLKMDLFMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYADIMDITEQLVSGMVKAIRGSYKVIYHPEGPEGPEQELDFTPPFKRVSMIKTLEEKLQVKFPAADTFNSPETNQFLSQLCAKHQVECPAPRTTARLLDKLVGEFIEEFCVNPTFICEHPQIMSPLAKYHRSIPGLTERFELFVAKKEICNAYTELNDPVVQRERFEQQASDKAAGDDEAQLVDENFCTSLEYGLPPTGGFGMGIDRLAMFLTDSNNIKEVLLFPAMKPEDANRTAAETAPTAQQ

Target ORF information:

RefSeq Version	NM_132345.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lysyl-tRNA synthetase (LysRS), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132345.2

ORF Insert Sequence:

ATGGCCGCTG CAAGTGGAGA ACTGTCCAAG AATGAGCTAA AGCGTCGCCT GAAGGCCGAG 
CAAAAGGCCA AGGAGAAGGC TGAGAAGGCG GCCGCTGCGC CGGCGGAAAA TACTGCTGGC 
AAGAAGAAGA GCAGTGCCGC CGAGGAGGAG GAGATCTCGC CGAATGAATA CTTTAAGCTG 
CGCTCCGCCG CCGTTCAGGA GCTGAAACGT TCACCCGCAA CGGATCCCTA TCCACACAAG 
TTCCATGTGA GTAGCTCGCT GGAAGACTTC ATTGCCAAGT ACGAGAACAG CCTCAAGGAG 
GGCGAGACCC TGGAGAATGT CAAACTGAGC GTGGCTGGGC GCGTGCATGC CATACGCGAG 
TCGGGCGCCA AGCTCATTTT CTACGATCTG CGCGGCGAGG GCGTCAAGGT GCAGGTGATG 
GCCAGCGCTA AGTCCTACAA GTCGGAGGCG GATTTCGAAA TCGATACGTC CAAATTGCGA 
CGTGGTGATA TCATCGGCGT TGTCGGACAT CCCGGCAAAA CCAAGAAGGG TGAACTGTCC 
GTTATGCCCA GCGAGATCAA GCTGCTATCG CCCTGTCTAC ACATGCTGCC CCATCTGCAT 
TTCGGGTTGA AGGACAAGGA GACGCGCTAC CGCCAGCGCT ATCTTGATCT CATTCTCAAC 
AACAATGTGC GAGAGAAATT CCAGATCCGT GCCAAGATCA TCTCGTACGT GCGTCAGTTC 
CTGGATCGTC TGGGTTTCCT GGAGATCGAG ACGCCCATGA TGAACATGAT CGCCGGTGGA 
GCCACTGCCA AGCCCTTCGT GACGCATCAC AATGACCTCA AGATGGATCT GTTCATGCGC 
ATTGCTCCGG AGCTGTATCA CAAGATGCTG GTGGTGGGTG GACTCGATCG TGTGTACGAG 
ATCGGACGGC AGTTCCGCAA CGAGGGCATC GACCTCACCC ACAATCCTGA ATTCACCACG 
TGCGAGTTCT ACATGGCGTA TGCCGACTAC GCTGACATTA TGGATATCAC CGAGCAGTTG 
GTCTCGGGAA TGGTGAAAGC GATCCGTGGC TCCTACAAGG TCATCTATCA TCCGGAGGGT 
CCAGAGGGAC CTGAGCAAGA ACTTGACTTT ACGCCGCCCT TTAAGCGCGT ATCCATGATC 
AAAACGCTGG AAGAGAAGCT GCAGGTGAAG TTCCCCGCGG CCGACACCTT CAATTCGCCA 
GAGACGAATC AGTTCCTGTC TCAGCTGTGC GCTAAGCACC AGGTCGAGTG CCCAGCTCCG 
CGCACCACTG CCCGCCTGCT GGACAAGCTC GTCGGCGAAT TCATCGAGGA GTTTTGCGTG 
AATCCAACGT TCATCTGTGA GCATCCGCAG ATCATGTCGC CGCTGGCCAA GTACCATCGC 
AGTATTCCCG GCCTGACGGA GCGCTTCGAA CTGTTTGTGG CCAAGAAGGA GATTTGCAAC 
GCGTATACCG AGTTGAACGA TCCGGTTGTG CAGCGCGAGC GTTTCGAGCA GCAGGCCAGC 
GACAAGGCGG CCGGCGATGA TGAGGCCCAG CTGGTCGACG AGAACTTCTG CACGTCCCTG 
GAGTACGGAC TGCCGCCCAC CGGTGGTTTT GGCATGGGCA TCGATCGCCT GGCCATGTTC 
CTCACGGACT CCAACAACAT CAAGGAGGTT CTGCTGTTCC CCGCCATGAA ACCGGAGGAT 
GCCAACCGCA CGGCAGCGGA AACTGCTCCC ACCGCACAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LysRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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