GLS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLS2
Entrez Gene ID	27165
Full Name	glutaminase 2
Synonyms	GA,GLS,LGA,hLGA
General protein information	Preferred Names glutaminase 2 Names glutaminase liver isoform, mitochondrial L-glutamine amidohydrolase breast cell glutaminase glutaminase 2 (liver, mitochondrial) glutaminase I phosphate-activated glutaminase phosphate-dependent glutaminase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.3
Summary	The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013].
Disorder MIM:	606365

mRNA and Protein(s)

mRNA	Protein	Name
XM_017019180.1	XP_016874669.1	glutaminase liver isoform, mitochondrial isoform X2
NM_001280798.1	NP_001267727.1	glutaminase liver isoform, mitochondrial isoform 4
NM_013267.3	NP_037399.2	glutaminase liver isoform, mitochondrial isoform 1 precursor
NM_013267.4	NP_037399.2	glutaminase liver isoform, mitochondrial isoform 1 precursor
NM_001280797.1	NP_001267726.1	glutaminase liver isoform, mitochondrial isoform 4
NM_001280796.1	NP_001267725.1	glutaminase liver isoform, mitochondrial isoform 3
XM_005268797.1	XP_005268854.1	glutaminase liver isoform, mitochondrial isoform X1
NM_001280796.2	NP_001267725.1	glutaminase liver isoform, mitochondrial isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLS2	NP_037399.2
Pan troglodytes (chimpanzee)	GLS2	XP_509148.2
Rattus norvegicus (Norway rat)	Gls2	NP_001257715.1
Danio rerio (zebrafish)	gls2	XP_001345099.5
Xenopus tropicalis (tropical clawed frog)	gls2	XP_002934555.2
Bos taurus (cattle)	GLS2	NP_001179203.1
Macaca mulatta (Rhesus monkey)	GLS2	XP_001115245.1
Danio rerio (zebrafish)	si:dkey-102c8.6	NP_001077294.1
Mus musculus (house mouse)	Gls2	NP_001028436.2
Canis lupus familiaris (dog)	GLS2	XP_003431516.1

Gene Ontology
Bibliography

Related articles in PubMed

Human genetic variation in GLS2 is associated with development of complicated Staphylococcus aureus bacteremia.
Scott WK, Medie FM, Ruffin F, Sharma-Kuinkel BK, Cyr DD, Guo S, Dykxhoorn DM, Skov RL, Bruun NE, Dahl A, Lerche CJ, Petersen A, Larsen AR, Lauridsen TK, Johansen HK, Ullum H, Sørensen E, Hassager C, Bundgaard H, Schønheyder HC, Torp-Pedersen C, Østergaard LB, Arpi M, Rosenvinge F, Erikstrup LT, Chehri M, Søgaard P, Andersen PS, Fowler VG Jr
PLoS genetics14(10)e1007667(2018 10)

The origin and evolution of human glutaminases and their atypical C-terminal ankyrin repeats.
Pasquali CC, Islam Z, Adamoski D, Ferreira IM, Righeto RD, Bettini J, Portugal RV, Yue WW, Gonzalez A, Dias SMG, Ambrosio ALB
The Journal of biological chemistry292(27)11572-11585(2017 07)

Glutaminase 2 stabilizes Dicer to repress Snail and metastasis in hepatocellular carcinoma cells.
Kuo TC, Chen CK, Hua KT, Yu P, Lee WJ, Chen MW, Jeng YM, Chien MH, Kuo KT, Hsiao M, Kuo ML
Cancer letters383(2)282-294(2016 12)

CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.
Masamha CP, Xia Z, Peart N, Collum S, Li W, Wagner EJ, Shyu AB
RNA (New York, N.Y.)22(6)830-8(2016 06)

The combination of three molecular markers can be a valuable predictive tool for the prognosis of hepatocellular carcinoma patients.
Chen SS, Yu KK, Ling QX, Huang C, Li N, Zheng JM, Bao SX, Cheng Q, Zhu MQ, Chen MQ
Scientific reports624582(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GLS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25275	NM_013267.4 Latest version!	Homo sapiens glutaminase 2 (GLS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$249.50-$349.30 $499.00
OHu25140	NM_001280796.2 Latest version!	Homo sapiens glutaminase 2 (GLS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu46483	XM_005268797.1 Latest version!	Homo sapiens glutaminase 2 (GLS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu25114	NM_001280798.1 Latest version!	Homo sapiens glutaminase 2 (GLS2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu25114	NM_001280797.1 Latest version!	Homo sapiens glutaminase 2 (GLS2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu25114	XM_017019180.1 Latest version!	Homo sapiens glutaminase 2 (GLS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu25275	NM_013267.3	Homo sapiens glutaminase 2 (GLS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$249.50-$349.30 $499.00
OHu25140	NM_001280796.1	Homo sapiens glutaminase 2 (GLS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLS2 ( NM_013267.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037399.2 Homo sapiens glutaminase 2 (GLS2), transcript variant 1, mRNA.

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CloneID	OHu25275
Clone ID Related Accession (Same CDS sequence)	NM_013267.3 , NM_013267.4
Accession Version	NM_013267.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509465600000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA180204.1, AF223944.1, AF110331.1 and AI241400.1. On Jul 23, 2013 this sequence version replaced NM_013267.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.110100.1, SRR1660809.231194.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTCCA TGAAGGCTCT GCAGAAGGCC CTGAGCCGGG CTGGCAGTCA CTGCGGGCGA 
GGAGGCTGGG GTCACCCGAG CCGGAGCCCC CTCCTTGGCG GGGGCGTCCG GCACCACCTC 
AGTGAGGCCG CGGCGCAGGG CAGAGAGACG CCACACAGCC ACCAGCCGCA GCACCAGGAT 
CATGATTCAT CAGAAAGTGG CATGCTGTCC CGCCTGGGTG ATTTGCTCTT TTACACTATT 
GCTGAAGGAC AGGAACGAAT CCCTATCCAC AAGTTCACCA CTGCACTAAA GGCCACTGGA 
CTGCAGACAT CAGATCCTCG GCTCCGAGAC TGCATGAGCG AGATGCACCG CGTGGTCCAA 
GAGTCCAGTA GTGGTGGCCT CTTGGACCGA GATCTCTTCC GAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACCCAGGC ATTCCGAAAG AAGTTTGTCA TTCCTGATTT TGAGGAGTTC 
ACGGGCCATG TGGATCGCAT CTTTGAGGAT GTCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCAGCTGGC CAAGTCAAAC CCAGACCTGT GGGGTGTCTC CCTGTGCACT 
GTGGATGGTC AACGGCACTC TGTGGGCCAC ACAAAGATCC CCTTCTGCCT GCAGTCCTGT 
GTGAAGCCCC TCACCTATGC CATCTCCATA AGCACCCTAG GCACTGACTA CGTGCACAAG 
TTTGTGGGCA AAGAGCCAAG TGGCCTGCGC TACAACAAGC TCTCCCTCAA TGAGGAAGGA 
ATCCCCCATA ACCCCATGGT CAATGCTGGT GCCATTGTTG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGATTTT GTGTTGCAGT ATCTCAACAA AATGGCTGGG 
AATGAATACA TGGGTTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAAAC AGGGGATCGG 
AATTATGCCA TCGGCTATTA TCTCAAGGAA AAGAAGTGCT TTCCTAAGGG GGTGGACATG 
ATGGCTGCCC TTGATCTCTA CTTCCAGCTG TGTTCTGTGG AGGTCACTTG TGAATCAGGC 
AGTGTCATGG CAGCCACCCT CGCCAACGGT GGGATCTGCC CCATCACAGG CGAGAGTGTG 
CTGAGTGCTG AAGCAGTGCG CAACACCCTC AGCCTCATGC ATTCCTGCGG CATGTATGAC 
TTCTCTGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT ATCAGGAGCC 
ATCCTCCTGG TGGTACCCAA TGTCATGGGA ATGATGTGCC TGTCACCCCC ATTGGACAAG 
CTGGGGAACA GCCATAGGGG GACCAGCTTC TGCCAGAAGT TGGTGTCTCT CTTCAATTTC 
CACAACTATG ACAACCTGAG GCACTGTGCT CGGAAGTTAG ACCCACGGCG TGAAGGGGCA 
GAAATTCGGA ACAAGACTGT GGTCAACCTG TTATTTGCTG CCTATAGTGG CGATGTCTCA 
GCTCTTCGAA GGTTTGCCTT GTCAGCCATG GATATGGAAC AGAAAGACTA TGACTCGCGC 
ACAGCTCTGC ATGTTGCTGC AGCTGAAGGA CACATCGAAG TTGTTAAATT CCTGATCGAG 
GCTTGCAAAG TGAATCCTTT TGCCAAGGAC AGGTGGGGCA ACATTCCCCT GGATGATGCT 
GTGCAGTTCA ACCATCTGGA GGTGGTCAAA CTGCTTCAAG ATTACCAGGA CTCCTACACA 
CTCTCTGAAA CTCAGGCTGA GGCAGCAGCT GAGGCCCTGT CCAAAGAGAA CTTAGAAAGC 
ATGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037399.2
CDS	297..2105
Translation	MRSMKALQKALSRAGSHCGRGGWGHPSRSPLLGGGVRHHLSEAAAQGRETPHSHQPQHQDHDSSESGMLSRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLRDCMSEMHRVVQESSSGGLLDRDLFRKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDVKELTGGKVAAYIPQLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISISTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	NM_013267.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013267.3

ORF Insert Sequence:

ATGCGCTCCA TGAAGGCTCT GCAGAAGGCC CTGAGCCGGG CTGGCAGTCA CTGCGGGCGA 
GGAGGCTGGG GTCACCCGAG CCGGAGCCCC CTCCTTGGCG GGGGCGTCCG GCACCACCTC 
AGTGAGGCCG CGGCGCAGGG CAGAGAGACG CCACACAGCC ACCAGCCGCA GCACCAGGAT 
CATGATTCAT CAGAAAGTGG CATGCTGTCC CGCCTGGGTG ATTTGCTCTT TTACACTATT 
GCTGAAGGAC AGGAACGAAT CCCTATCCAC AAGTTCACCA CTGCACTAAA GGCCACTGGA 
CTGCAGACAT CAGATCCTCG GCTCCGAGAC TGCATGAGCG AGATGCACCG CGTGGTCCAA 
GAGTCCAGTA GTGGTGGCCT CTTGGACCGA GATCTCTTCC GAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACCCAGGC ATTCCGAAAG AAGTTTGTCA TTCCTGATTT TGAGGAGTTC 
ACGGGCCATG TGGATCGCAT CTTTGAGGAT GTCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCAGCTGGC CAAGTCAAAC CCAGACCTGT GGGGTGTCTC CCTGTGCACT 
GTGGATGGTC AACGGCACTC TGTGGGCCAC ACAAAGATCC CCTTCTGCCT GCAGTCCTGT 
GTGAAGCCCC TCACCTATGC CATCTCCATA AGCACCCTAG GCACTGACTA CGTGCACAAG 
TTTGTGGGCA AAGAGCCAAG TGGCCTGCGC TACAACAAGC TCTCCCTCAA TGAGGAAGGA 
ATCCCCCATA ACCCCATGGT CAATGCTGGT GCCATTGTTG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGATTTT GTGTTGCAGT ATCTCAACAA AATGGCTGGG 
AATGAATACA TGGGTTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAAAC AGGGGATCGG 
AATTATGCCA TCGGCTATTA TCTCAAGGAA AAGAAGTGCT TTCCTAAGGG GGTGGACATG 
ATGGCTGCCC TTGATCTCTA CTTCCAGCTG TGTTCTGTGG AGGTCACTTG TGAATCAGGC 
AGTGTCATGG CAGCCACCCT CGCCAACGGT GGGATCTGCC CCATCACAGG CGAGAGTGTG 
CTGAGTGCTG AAGCAGTGCG CAACACCCTC AGCCTCATGC ATTCCTGCGG CATGTATGAC 
TTCTCTGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT ATCAGGAGCC 
ATCCTCCTGG TGGTACCCAA TGTCATGGGA ATGATGTGCC TGTCACCCCC ATTGGACAAG 
CTGGGGAACA GCCATAGGGG GACCAGCTTC TGCCAGAAGT TGGTGTCTCT CTTCAATTTC 
CACAACTATG ACAACCTGAG GCACTGTGCT CGGAAGTTAG ACCCACGGCG TGAAGGGGCA 
GAAATTCGGA ACAAGACTGT GGTCAACCTG TTATTTGCTG CCTATAGTGG CGATGTCTCA 
GCTCTTCGAA GGTTTGCCTT GTCAGCCATG GATATGGAAC AGAAAGACTA TGACTCGCGC 
ACAGCTCTGC ATGTTGCTGC AGCTGAAGGA CACATCGAAG TTGTTAAATT CCTGATCGAG 
GCTTGCAAAG TGAATCCTTT TGCCAAGGAC AGGTGGGGCA ACATTCCCCT GGATGATGCT 
GTGCAGTTCA ACCATCTGGA GGTGGTCAAA CTGCTTCAAG ATTACCAGGA CTCCTACACA 
CTCTCTGAAA CTCAGGCTGA GGCAGCAGCT GAGGCCCTGT CCAAAGAGAA CTTAGAAAGC 
ATGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLS2 ( NM_013267.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037399.2 Homo sapiens glutaminase 2 (GLS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25275
Clone ID Related Accession (Same CDS sequence)	NM_013267.3 , NM_013267.4
Accession Version	NM_013267.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF223944.1 and AF110331.1. On Nov 23, 2018 this sequence version replaced NM_013267.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000467.1, AF223944.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000311966.9/ ENSP00000310447.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGCTCCA TGAAGGCTCT GCAGAAGGCC CTGAGCCGGG CTGGCAGTCA CTGCGGGCGA 
GGAGGCTGGG GTCACCCGAG CCGGAGCCCC CTCCTTGGCG GGGGCGTCCG GCACCACCTC 
AGTGAGGCCG CGGCGCAGGG CAGAGAGACG CCACACAGCC ACCAGCCGCA GCACCAGGAT 
CATGATTCAT CAGAAAGTGG CATGCTGTCC CGCCTGGGTG ATTTGCTCTT TTACACTATT 
GCTGAAGGAC AGGAACGAAT CCCTATCCAC AAGTTCACCA CTGCACTAAA GGCCACTGGA 
CTGCAGACAT CAGATCCTCG GCTCCGAGAC TGCATGAGCG AGATGCACCG CGTGGTCCAA 
GAGTCCAGTA GTGGTGGCCT CTTGGACCGA GATCTCTTCC GAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACCCAGGC ATTCCGAAAG AAGTTTGTCA TTCCTGATTT TGAGGAGTTC 
ACGGGCCATG TGGATCGCAT CTTTGAGGAT GTCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCAGCTGGC CAAGTCAAAC CCAGACCTGT GGGGTGTCTC CCTGTGCACT 
GTGGATGGTC AACGGCACTC TGTGGGCCAC ACAAAGATCC CCTTCTGCCT GCAGTCCTGT 
GTGAAGCCCC TCACCTATGC CATCTCCATA AGCACCCTAG GCACTGACTA CGTGCACAAG 
TTTGTGGGCA AAGAGCCAAG TGGCCTGCGC TACAACAAGC TCTCCCTCAA TGAGGAAGGA 
ATCCCCCATA ACCCCATGGT CAATGCTGGT GCCATTGTTG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGATTTT GTGTTGCAGT ATCTCAACAA AATGGCTGGG 
AATGAATACA TGGGTTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAAAC AGGGGATCGG 
AATTATGCCA TCGGCTATTA TCTCAAGGAA AAGAAGTGCT TTCCTAAGGG GGTGGACATG 
ATGGCTGCCC TTGATCTCTA CTTCCAGCTG TGTTCTGTGG AGGTCACTTG TGAATCAGGC 
AGTGTCATGG CAGCCACCCT CGCCAACGGT GGGATCTGCC CCATCACAGG CGAGAGTGTG 
CTGAGTGCTG AAGCAGTGCG CAACACCCTC AGCCTCATGC ATTCCTGCGG CATGTATGAC 
TTCTCTGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT ATCAGGAGCC 
ATCCTCCTGG TGGTACCCAA TGTCATGGGA ATGATGTGCC TGTCACCCCC ATTGGACAAG 
CTGGGGAACA GCCATAGGGG GACCAGCTTC TGCCAGAAGT TGGTGTCTCT CTTCAATTTC 
CACAACTATG ACAACCTGAG GCACTGTGCT CGGAAGTTAG ACCCACGGCG TGAAGGGGCA 
GAAATTCGGA ACAAGACTGT GGTCAACCTG TTATTTGCTG CCTATAGTGG CGATGTCTCA 
GCTCTTCGAA GGTTTGCCTT GTCAGCCATG GATATGGAAC AGAAAGACTA TGACTCGCGC 
ACAGCTCTGC ATGTTGCTGC AGCTGAAGGA CACATCGAAG TTGTTAAATT CCTGATCGAG 
GCTTGCAAAG TGAATCCTTT TGCCAAGGAC AGGTGGGGCA ACATTCCCCT GGATGATGCT 
GTGCAGTTCA ACCATCTGGA GGTGGTCAAA CTGCTTCAAG ATTACCAGGA CTCCTACACA 
CTCTCTGAAA CTCAGGCTGA GGCAGCAGCT GAGGCCCTGT CCAAAGAGAA CTTAGAAAGC 
ATGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037399.2
CDS	44..1852
Translation	MRSMKALQKALSRAGSHCGRGGWGHPSRSPLLGGGVRHHLSEAAAQGRETPHSHQPQHQDHDSSESGMLSRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLRDCMSEMHRVVQESSSGGLLDRDLFRKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDVKELTGGKVAAYIPQLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISISTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	NM_013267.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013267.4

ORF Insert Sequence:

ATGCGCTCCA TGAAGGCTCT GCAGAAGGCC CTGAGCCGGG CTGGCAGTCA CTGCGGGCGA 
GGAGGCTGGG GTCACCCGAG CCGGAGCCCC CTCCTTGGCG GGGGCGTCCG GCACCACCTC 
AGTGAGGCCG CGGCGCAGGG CAGAGAGACG CCACACAGCC ACCAGCCGCA GCACCAGGAT 
CATGATTCAT CAGAAAGTGG CATGCTGTCC CGCCTGGGTG ATTTGCTCTT TTACACTATT 
GCTGAAGGAC AGGAACGAAT CCCTATCCAC AAGTTCACCA CTGCACTAAA GGCCACTGGA 
CTGCAGACAT CAGATCCTCG GCTCCGAGAC TGCATGAGCG AGATGCACCG CGTGGTCCAA 
GAGTCCAGTA GTGGTGGCCT CTTGGACCGA GATCTCTTCC GAAAGTGTGT GAGCAGCAAC 
ATTGTGCTCC TGACCCAGGC ATTCCGAAAG AAGTTTGTCA TTCCTGATTT TGAGGAGTTC 
ACGGGCCATG TGGATCGCAT CTTTGAGGAT GTCAAAGAGC TCACTGGAGG CAAAGTGGCA 
GCCTACATCC CTCAGCTGGC CAAGTCAAAC CCAGACCTGT GGGGTGTCTC CCTGTGCACT 
GTGGATGGTC AACGGCACTC TGTGGGCCAC ACAAAGATCC CCTTCTGCCT GCAGTCCTGT 
GTGAAGCCCC TCACCTATGC CATCTCCATA AGCACCCTAG GCACTGACTA CGTGCACAAG 
TTTGTGGGCA AAGAGCCAAG TGGCCTGCGC TACAACAAGC TCTCCCTCAA TGAGGAAGGA 
ATCCCCCATA ACCCCATGGT CAATGCTGGT GCCATTGTTG TCAGCTCCCT GATCAAGATG 
GACTGTAACA AAGCAGAGAA GTTTGATTTT GTGTTGCAGT ATCTCAACAA AATGGCTGGG 
AATGAATACA TGGGTTTCAG CAATGCCACA TTCCAGTCAG AGAAGGAAAC AGGGGATCGG 
AATTATGCCA TCGGCTATTA TCTCAAGGAA AAGAAGTGCT TTCCTAAGGG GGTGGACATG 
ATGGCTGCCC TTGATCTCTA CTTCCAGCTG TGTTCTGTGG AGGTCACTTG TGAATCAGGC 
AGTGTCATGG CAGCCACCCT CGCCAACGGT GGGATCTGCC CCATCACAGG CGAGAGTGTG 
CTGAGTGCTG AAGCAGTGCG CAACACCCTC AGCCTCATGC ATTCCTGCGG CATGTATGAC 
TTCTCTGGCC AGTTTGCCTT CCACGTGGGC CTGCCAGCCA AGTCAGCTGT ATCAGGAGCC 
ATCCTCCTGG TGGTACCCAA TGTCATGGGA ATGATGTGCC TGTCACCCCC ATTGGACAAG 
CTGGGGAACA GCCATAGGGG GACCAGCTTC TGCCAGAAGT TGGTGTCTCT CTTCAATTTC 
CACAACTATG ACAACCTGAG GCACTGTGCT CGGAAGTTAG ACCCACGGCG TGAAGGGGCA 
GAAATTCGGA ACAAGACTGT GGTCAACCTG TTATTTGCTG CCTATAGTGG CGATGTCTCA 
GCTCTTCGAA GGTTTGCCTT GTCAGCCATG GATATGGAAC AGAAAGACTA TGACTCGCGC 
ACAGCTCTGC ATGTTGCTGC AGCTGAAGGA CACATCGAAG TTGTTAAATT CCTGATCGAG 
GCTTGCAAAG TGAATCCTTT TGCCAAGGAC AGGTGGGGCA ACATTCCCCT GGATGATGCT 
GTGCAGTTCA ACCATCTGGA GGTGGTCAAA CTGCTTCAAG ATTACCAGGA CTCCTACACA 
CTCTCTGAAA CTCAGGCTGA GGCAGCAGCT GAGGCCCTGT CCAAAGAGAA CTTAGAAAGC 
ATGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLS2 ( NM_001280796.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001267725.1 Homo sapiens glutaminase 2 (GLS2), transcript variant 3, mRNA.

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CloneID	OHu25140
Clone ID Related Accession (Same CDS sequence)	NM_001280796.1 , NM_001280796.2
Accession Version	NM_001280796.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA180204.1, BC048344.1 and AI241400.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, uses an alternate start codon, and uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform 3 which has a distinct and shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048344.1, SRR1803614.218192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATACAT GGGTTTCAGC AATGCCACGG AAGGTAATTT TTCCCCTTCT CCCAACACCC 
ACTCCTCCAA CTTCAAGTAC TAAATTGGGT GTTTGCTTCA GATTCCAGTC AGAGAAGGAA 
ACAGGGGATC GGAATTATGC CATCGGCTAT TATCTCAAGG AAAAGAAGTG CTTTCCTAAG 
GGGGTGGACA TGATGGCTGC CCTTGATCTC TACTTCCAGC TGTGTTCTGT GGAGGTCACT 
TGTGAATCAG GCAGTGTCAT GGCAGCCACC CTCGCCAACG GTGGGATCTG CCCCATCACA 
GGCGAGAGTG TGCTGAGTGC TGAAGCAGTG CGCAACACCC TCAGCCTCAT GCATTCCTGC 
GGCATGTATG ACTTCTCTGG CCAGTTTGCC TTCCACGTGG GCCTGCCAGC CAAGTCAGCT 
GTATCAGGAG CCATCCTCCT GGTGGTACCC AATGTCATGG GAATGATGTG CCTGTCACCC 
CCATTGGACA AGCTGGGGAA CAGCCATAGG GGGACCAGCT TCTGCCAGAA GTTGGTGTCT 
CTCTTCAATT TCCACAACTA TGACAACCTG AGGCACTGTG CTCGGAAGTT AGACCCACGG 
CGTGAAGGGG CAGAAATTCG GAACAAGACT GTGGTCAACC TGTTATTTGC TGCCTATAGT 
GGCGATGTCT CAGCTCTTCG AAGGTTTGCC TTGTCAGCCA TGGATATGGA ACAGAAAGAC 
TATGACTCGC GCACAGCTCT GCATGTTGCT GCAGCTGAAG GACACATCGA AGTTGTTAAA 
TTCCTGATCG AGGCTTGCAA AGTGAATCCT TTTGCCAAGG ACAGGTGGGG CAACATTCCC 
CTGGATGATG CTGTGCAGTT CAACCATCTG GAGGTGGTCA AACTGCTTCA AGATTACCAG 
GACTCCTACA CACTCTCTGA AACTCAGGCT GAGGCAGCAG CTGAGGCCCT GTCCAAAGAG 
AACTTAGAAA GCATGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267725.1
CDS	1198..2178
Translation	MNTWVSAMPRKVIFPLLPTPTPPTSSTKLGVCFRFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	NM_001280796.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280796.1

ORF Insert Sequence:

ATGAATACAT GGGTTTCAGC AATGCCACGG AAGGTAATTT TTCCCCTTCT CCCAACACCC 
ACTCCTCCAA CTTCAAGTAC TAAATTGGGT GTTTGCTTCA GATTCCAGTC AGAGAAGGAA 
ACAGGGGATC GGAATTATGC CATCGGCTAT TATCTCAAGG AAAAGAAGTG CTTTCCTAAG 
GGGGTGGACA TGATGGCTGC CCTTGATCTC TACTTCCAGC TGTGTTCTGT GGAGGTCACT 
TGTGAATCAG GCAGTGTCAT GGCAGCCACC CTCGCCAACG GTGGGATCTG CCCCATCACA 
GGCGAGAGTG TGCTGAGTGC TGAAGCAGTG CGCAACACCC TCAGCCTCAT GCATTCCTGC 
GGCATGTATG ACTTCTCTGG CCAGTTTGCC TTCCACGTGG GCCTGCCAGC CAAGTCAGCT 
GTATCAGGAG CCATCCTCCT GGTGGTACCC AATGTCATGG GAATGATGTG CCTGTCACCC 
CCATTGGACA AGCTGGGGAA CAGCCATAGG GGGACCAGCT TCTGCCAGAA GTTGGTGTCT 
CTCTTCAATT TCCACAACTA TGACAACCTG AGGCACTGTG CTCGGAAGTT AGACCCACGG 
CGTGAAGGGG CAGAAATTCG GAACAAGACT GTGGTCAACC TGTTATTTGC TGCCTATAGT 
GGCGATGTCT CAGCTCTTCG AAGGTTTGCC TTGTCAGCCA TGGATATGGA ACAGAAAGAC 
TATGACTCGC GCACAGCTCT GCATGTTGCT GCAGCTGAAG GACACATCGA AGTTGTTAAA 
TTCCTGATCG AGGCTTGCAA AGTGAATCCT TTTGCCAAGG ACAGGTGGGG CAACATTCCC 
CTGGATGATG CTGTGCAGTT CAACCATCTG GAGGTGGTCA AACTGCTTCA AGATTACCAG 
GACTCCTACA CACTCTCTGA AACTCAGGCT GAGGCAGCAG CTGAGGCCCT GTCCAAAGAG 
AACTTAGAAA GCATGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLS2 ( NM_001280796.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001267725.1 Homo sapiens glutaminase 2 (GLS2), transcript variant 3, mRNA.

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CloneID	OHu25140
Clone ID Related Accession (Same CDS sequence)	NM_001280796.1 , NM_001280796.2
Accession Version	NM_001280796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC048344.1 and AI241400.1. On May 31, 2019 this sequence version replaced NM_001280796.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, uses an alternate start codon, and uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform 3 which has a distinct and shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048344.1, SRR1803614.218192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATACAT GGGTTTCAGC AATGCCACGG AAGGTAATTT TTCCCCTTCT CCCAACACCC 
ACTCCTCCAA CTTCAAGTAC TAAATTGGGT GTTTGCTTCA GATTCCAGTC AGAGAAGGAA 
ACAGGGGATC GGAATTATGC CATCGGCTAT TATCTCAAGG AAAAGAAGTG CTTTCCTAAG 
GGGGTGGACA TGATGGCTGC CCTTGATCTC TACTTCCAGC TGTGTTCTGT GGAGGTCACT 
TGTGAATCAG GCAGTGTCAT GGCAGCCACC CTCGCCAACG GTGGGATCTG CCCCATCACA 
GGCGAGAGTG TGCTGAGTGC TGAAGCAGTG CGCAACACCC TCAGCCTCAT GCATTCCTGC 
GGCATGTATG ACTTCTCTGG CCAGTTTGCC TTCCACGTGG GCCTGCCAGC CAAGTCAGCT 
GTATCAGGAG CCATCCTCCT GGTGGTACCC AATGTCATGG GAATGATGTG CCTGTCACCC 
CCATTGGACA AGCTGGGGAA CAGCCATAGG GGGACCAGCT TCTGCCAGAA GTTGGTGTCT 
CTCTTCAATT TCCACAACTA TGACAACCTG AGGCACTGTG CTCGGAAGTT AGACCCACGG 
CGTGAAGGGG CAGAAATTCG GAACAAGACT GTGGTCAACC TGTTATTTGC TGCCTATAGT 
GGCGATGTCT CAGCTCTTCG AAGGTTTGCC TTGTCAGCCA TGGATATGGA ACAGAAAGAC 
TATGACTCGC GCACAGCTCT GCATGTTGCT GCAGCTGAAG GACACATCGA AGTTGTTAAA 
TTCCTGATCG AGGCTTGCAA AGTGAATCCT TTTGCCAAGG ACAGGTGGGG CAACATTCCC 
CTGGATGATG CTGTGCAGTT CAACCATCTG GAGGTGGTCA AACTGCTTCA AGATTACCAG 
GACTCCTACA CACTCTCTGA AACTCAGGCT GAGGCAGCAG CTGAGGCCCT GTCCAAAGAG 
AACTTAGAAA GCATGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267725.1
CDS	945..1925
Translation	MNTWVSAMPRKVIFPLLPTPTPPTSSTKLGVCFRFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	NM_001280796.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280796.2

ORF Insert Sequence:

ATGAATACAT GGGTTTCAGC AATGCCACGG AAGGTAATTT TTCCCCTTCT CCCAACACCC 
ACTCCTCCAA CTTCAAGTAC TAAATTGGGT GTTTGCTTCA GATTCCAGTC AGAGAAGGAA 
ACAGGGGATC GGAATTATGC CATCGGCTAT TATCTCAAGG AAAAGAAGTG CTTTCCTAAG 
GGGGTGGACA TGATGGCTGC CCTTGATCTC TACTTCCAGC TGTGTTCTGT GGAGGTCACT 
TGTGAATCAG GCAGTGTCAT GGCAGCCACC CTCGCCAACG GTGGGATCTG CCCCATCACA 
GGCGAGAGTG TGCTGAGTGC TGAAGCAGTG CGCAACACCC TCAGCCTCAT GCATTCCTGC 
GGCATGTATG ACTTCTCTGG CCAGTTTGCC TTCCACGTGG GCCTGCCAGC CAAGTCAGCT 
GTATCAGGAG CCATCCTCCT GGTGGTACCC AATGTCATGG GAATGATGTG CCTGTCACCC 
CCATTGGACA AGCTGGGGAA CAGCCATAGG GGGACCAGCT TCTGCCAGAA GTTGGTGTCT 
CTCTTCAATT TCCACAACTA TGACAACCTG AGGCACTGTG CTCGGAAGTT AGACCCACGG 
CGTGAAGGGG CAGAAATTCG GAACAAGACT GTGGTCAACC TGTTATTTGC TGCCTATAGT 
GGCGATGTCT CAGCTCTTCG AAGGTTTGCC TTGTCAGCCA TGGATATGGA ACAGAAAGAC 
TATGACTCGC GCACAGCTCT GCATGTTGCT GCAGCTGAAG GACACATCGA AGTTGTTAAA 
TTCCTGATCG AGGCTTGCAA AGTGAATCCT TTTGCCAAGG ACAGGTGGGG CAACATTCCC 
CTGGATGATG CTGTGCAGTT CAACCATCTG GAGGTGGTCA AACTGCTTCA AGATTACCAG 
GACTCCTACA CACTCTCTGA AACTCAGGCT GAGGCAGCAG CTGAGGCCCT GTCCAAAGAG 
AACTTAGAAA GCATGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLS2 ( XM_005268797.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268854.1 Homo sapiens glutaminase 2 (GLS2), transcript variant X1, mRNA.

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CloneID	OHu46483
Clone ID Related Accession (Same CDS sequence)	XM_005268797.1
Accession Version	XM_005268797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACA GCCTCCTGCC TCCGGTCCAG GTGTACTGGG GTCTGTGTGT TGTGTTTCTG 
CGTGTTCTCG GCAGTGATTC ATCAGAAAGT GGCATGCTGT CCCGCCTGGG TGATTTGCTC 
TTTTACACTA TTGCTGAAGG ACAGGAACGA ATCCCTATCC ACAAGTTCAC CACTGCACTA 
AAGGCCACTG GACTGCAGAC ATCAGATCCT CGGCTCCGAG ACTGCATGAG CGAGATGCAC 
CGCGTGGTCC AAGAGTCCAG TAGTGGTGGC CTCTTGGACC GAGATCTCTT CCGAAAGTGT 
GTGAGCAGCA ACATTGTGCT CCTGACCCAG GCATTCCGAA AGAAGTTTGT CATTCCTGAT 
TTTGAGGAGT TCACGGGCCA TGTGGATCGC ATCTTTGAGG ATGTCAAAGA GCTCACTGGA 
GGCAAAGTGG CAGCCTACAT CCCTCAGCTG GCCAAGTCAA ACCCAGACCT GTGGGGTGTC 
TCCCTGTGCA CTGTGGATGG TCAACGGCAC TCTGTGGGCC ACACAAAGAT CCCCTTCTGC 
CTGCAGTCCT GTGTGAAGCC CCTCACCTAT GCCATCTCCA TAAGCACCCT AGGCACTGAC 
TACGTGCACA AGTTTGTGGG CAAAGAGCCA AGTGGCCTGC GCTACAACAA GCTCTCCCTC 
AATGAGGAAG GAATCCCCCA TAACCCCATG GTCAATGCTG GTGCCATTGT TGTCAGCTCC 
CTGATCAAGA TGGACTGTAA CAAAGCAGAG AAGTTTGATT TTGTGTTGCA GTATCTCAAC 
AAAATGGCTG GGAATGAATA CATGGGTTTC AGCAATGCCA CATTCCAGTC AGAGAAGGAA 
ACAGGGGATC GGAATTATGC CATCGGCTAT TATCTCAAGG AAAAGAAGTG CTTTCCTAAG 
GGGGTGGACA TGATGGCTGC CCTTGATCTC TACTTCCAGC TGTGTTCTGT GGAGGTCACT 
TGTGAATCAG GCAGTGTCAT GGCAGCCACC CTCGCCAACG GTGGGATCTG CCCCATCACA 
GGCGAGAGTG TGCTGAGTGC TGAAGCAGTG CGCAACACCC TCAGCCTCAT GCATTCCTGC 
GGCATGTATG ACTTCTCTGG CCAGTTTGCC TTCCACGTGG GCCTGCCAGC CAAGTCAGCT 
GTATCAGGAG CCATCCTCCT GGTGGTACCC AATGTCATGG GAATGATGTG CCTGTCACCC 
CCATTGGACA AGCTGGGGAA CAGCCATAGG GGGACCAGCT TCTGCCAGAA GTTGGTGTCT 
CTCTTCAATT TCCACAACTA TGACAACCTG AGGCACTGTG CTCGGAAGTT AGACCCACGG 
CGTGAAGGGG CAGAAATTCG GAACAAGACT GTGGTCAACC TGTTATTTGC TGCCTATAGT 
GGCGATGTCT CAGCTCTTCG AAGGTTTGCC TTGTCAGCCA TGGATATGGA ACAGAAAGAC 
TATGACTCGC GCACAGCTCT GCATGTTGCT GCAGCTGAAG GACACATCGA AGTTGTTAAA 
TTCCTGATCG AGGCTTGCAA AGTGAATCCT TTTGCCAAGG ACAGGTGGGG CAACATTCCC 
CTGGATGATG CTGTGCAGTT CAACCATCTG GAGGTGGTCA AACTGCTTCA AGATTACCAG 
GACTCCTACA CACTCTCTGA AACTCAGGCT GAGGCAGCAG CTGAGGCCCT GTCCAAAGAG 
AACTTAGAAA GCATGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268854.1
CDS	32..1732
Translation	MWNSLLPPVQVYWGLCVVFLRVLGSDSSESGMLSRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLRDCMSEMHRVVQESSSGGLLDRDLFRKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDVKELTGGKVAAYIPQLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISISTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	XM_005268797.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268797.1

ORF Insert Sequence:

ATGTGGAACA GCCTCCTGCC TCCGGTCCAG GTGTACTGGG GTCTGTGTGT TGTGTTTCTG 
CGTGTTCTCG GCAGTGATTC ATCAGAAAGT GGCATGCTGT CCCGCCTGGG TGATTTGCTC 
TTTTACACTA TTGCTGAAGG ACAGGAACGA ATCCCTATCC ACAAGTTCAC CACTGCACTA 
AAGGCCACTG GACTGCAGAC ATCAGATCCT CGGCTCCGAG ACTGCATGAG CGAGATGCAC 
CGCGTGGTCC AAGAGTCCAG TAGTGGTGGC CTCTTGGACC GAGATCTCTT CCGAAAGTGT 
GTGAGCAGCA ACATTGTGCT CCTGACCCAG GCATTCCGAA AGAAGTTTGT CATTCCTGAT 
TTTGAGGAGT TCACGGGCCA TGTGGATCGC ATCTTTGAGG ATGTCAAAGA GCTCACTGGA 
GGCAAAGTGG CAGCCTACAT CCCTCAGCTG GCCAAGTCAA ACCCAGACCT GTGGGGTGTC 
TCCCTGTGCA CTGTGGATGG TCAACGGCAC TCTGTGGGCC ACACAAAGAT CCCCTTCTGC 
CTGCAGTCCT GTGTGAAGCC CCTCACCTAT GCCATCTCCA TAAGCACCCT AGGCACTGAC 
TACGTGCACA AGTTTGTGGG CAAAGAGCCA AGTGGCCTGC GCTACAACAA GCTCTCCCTC 
AATGAGGAAG GAATCCCCCA TAACCCCATG GTCAATGCTG GTGCCATTGT TGTCAGCTCC 
CTGATCAAGA TGGACTGTAA CAAAGCAGAG AAGTTTGATT TTGTGTTGCA GTATCTCAAC 
AAAATGGCTG GGAATGAATA CATGGGTTTC AGCAATGCCA CATTCCAGTC AGAGAAGGAA 
ACAGGGGATC GGAATTATGC CATCGGCTAT TATCTCAAGG AAAAGAAGTG CTTTCCTAAG 
GGGGTGGACA TGATGGCTGC CCTTGATCTC TACTTCCAGC TGTGTTCTGT GGAGGTCACT 
TGTGAATCAG GCAGTGTCAT GGCAGCCACC CTCGCCAACG GTGGGATCTG CCCCATCACA 
GGCGAGAGTG TGCTGAGTGC TGAAGCAGTG CGCAACACCC TCAGCCTCAT GCATTCCTGC 
GGCATGTATG ACTTCTCTGG CCAGTTTGCC TTCCACGTGG GCCTGCCAGC CAAGTCAGCT 
GTATCAGGAG CCATCCTCCT GGTGGTACCC AATGTCATGG GAATGATGTG CCTGTCACCC 
CCATTGGACA AGCTGGGGAA CAGCCATAGG GGGACCAGCT TCTGCCAGAA GTTGGTGTCT 
CTCTTCAATT TCCACAACTA TGACAACCTG AGGCACTGTG CTCGGAAGTT AGACCCACGG 
CGTGAAGGGG CAGAAATTCG GAACAAGACT GTGGTCAACC TGTTATTTGC TGCCTATAGT 
GGCGATGTCT CAGCTCTTCG AAGGTTTGCC TTGTCAGCCA TGGATATGGA ACAGAAAGAC 
TATGACTCGC GCACAGCTCT GCATGTTGCT GCAGCTGAAG GACACATCGA AGTTGTTAAA 
TTCCTGATCG AGGCTTGCAA AGTGAATCCT TTTGCCAAGG ACAGGTGGGG CAACATTCCC 
CTGGATGATG CTGTGCAGTT CAACCATCTG GAGGTGGTCA AACTGCTTCA AGATTACCAG 
GACTCCTACA CACTCTCTGA AACTCAGGCT GAGGCAGCAG CTGAGGCCCT GTCCAAAGAG 
AACTTAGAAA GCATGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLS2 ( NM_001280798.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001267727.1 Homo sapiens glutaminase 2 (GLS2), transcript variant 5, mRNA.

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CloneID	OHu25114
Clone ID Related Accession (Same CDS sequence)	XM_017019180.1 , NM_001280798.1 , NM_001280797.1
Accession Version	NM_001280798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569254400000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA180204.1, DA060302.1, AF110331.1 and AI241400.1. Transcript Variant: This variant (5) differs in the 5' UTR and 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 4 which has a shorter N-terminus compared to isoform 1. Both variants 4 and 5 encode the same isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.146554.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCAATG CTGGTGCCAT TGTTGTCAGC TCCCTGATCA AGATGGACTG TAACAAAGCA 
GAGAAGTTTG ATTTTGTGTT GCAGTATCTC AACAAAATGG CTGGGAATGA ATACATGGGT 
TTCAGCAATG CCACATTCCA GTCAGAGAAG GAAACAGGGG ATCGGAATTA TGCCATCGGC 
TATTATCTCA AGGAAAAGAA GTGCTTTCCT AAGGGGGTGG ACATGATGGC TGCCCTTGAT 
CTCTACTTCC AGCTGTGTTC TGTGGAGGTC ACTTGTGAAT CAGGCAGTGT CATGGCAGCC 
ACCCTCGCCA ACGGTGGGAT CTGCCCCATC ACAGGCGAGA GTGTGCTGAG TGCTGAAGCA 
GTGCGCAACA CCCTCAGCCT CATGCATTCC TGCGGCATGT ATGACTTCTC TGGCCAGTTT 
GCCTTCCACG TGGGCCTGCC AGCCAAGTCA GCTGTATCAG GAGCCATCCT CCTGGTGGTA 
CCCAATGTCA TGGGAATGAT GTGCCTGTCA CCCCCATTGG ACAAGCTGGG GAACAGCCAT 
AGGGGGACCA GCTTCTGCCA GAAGTTGGTG TCTCTCTTCA ATTTCCACAA CTATGACAAC 
CTGAGGCACT GTGCTCGGAA GTTAGACCCA CGGCGTGAAG GGGCAGAAAT TCGGAACAAG 
ACTGTGGTCA ACCTGTTATT TGCTGCCTAT AGTGGCGATG TCTCAGCTCT TCGAAGGTTT 
GCCTTGTCAG CCATGGATAT GGAACAGAAA GACTATGACT CGCGCACAGC TCTGCATGTT 
GCTGCAGCTG AAGGACACAT CGAAGTTGTT AAATTCCTGA TCGAGGCTTG CAAAGTGAAT 
CCTTTTGCCA AGGACAGGTG GGGCAACATT CCCCTGGATG ATGCTGTGCA GTTCAACCAT 
CTGGAGGTGG TCAAACTGCT TCAAGATTAC CAGGACTCCT ACACACTCTC TGAAACTCAG 
GCTGAGGCAG CAGCTGAGGC CCTGTCCAAA GAGAACTTAG AAAGCATGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267727.1
CDS	740..1753
Translation	MVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	NM_001280798.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280798.1

ORF Insert Sequence:

ATGGTCAATG CTGGTGCCAT TGTTGTCAGC TCCCTGATCA AGATGGACTG TAACAAAGCA 
GAGAAGTTTG ATTTTGTGTT GCAGTATCTC AACAAAATGG CTGGGAATGA ATACATGGGT 
TTCAGCAATG CCACATTCCA GTCAGAGAAG GAAACAGGGG ATCGGAATTA TGCCATCGGC 
TATTATCTCA AGGAAAAGAA GTGCTTTCCT AAGGGGGTGG ACATGATGGC TGCCCTTGAT 
CTCTACTTCC AGCTGTGTTC TGTGGAGGTC ACTTGTGAAT CAGGCAGTGT CATGGCAGCC 
ACCCTCGCCA ACGGTGGGAT CTGCCCCATC ACAGGCGAGA GTGTGCTGAG TGCTGAAGCA 
GTGCGCAACA CCCTCAGCCT CATGCATTCC TGCGGCATGT ATGACTTCTC TGGCCAGTTT 
GCCTTCCACG TGGGCCTGCC AGCCAAGTCA GCTGTATCAG GAGCCATCCT CCTGGTGGTA 
CCCAATGTCA TGGGAATGAT GTGCCTGTCA CCCCCATTGG ACAAGCTGGG GAACAGCCAT 
AGGGGGACCA GCTTCTGCCA GAAGTTGGTG TCTCTCTTCA ATTTCCACAA CTATGACAAC 
CTGAGGCACT GTGCTCGGAA GTTAGACCCA CGGCGTGAAG GGGCAGAAAT TCGGAACAAG 
ACTGTGGTCA ACCTGTTATT TGCTGCCTAT AGTGGCGATG TCTCAGCTCT TCGAAGGTTT 
GCCTTGTCAG CCATGGATAT GGAACAGAAA GACTATGACT CGCGCACAGC TCTGCATGTT 
GCTGCAGCTG AAGGACACAT CGAAGTTGTT AAATTCCTGA TCGAGGCTTG CAAAGTGAAT 
CCTTTTGCCA AGGACAGGTG GGGCAACATT CCCCTGGATG ATGCTGTGCA GTTCAACCAT 
CTGGAGGTGG TCAAACTGCT TCAAGATTAC CAGGACTCCT ACACACTCTC TGAAACTCAG 
GCTGAGGCAG CAGCTGAGGC CCTGTCCAAA GAGAACTTAG AAAGCATGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLS2 ( NM_001280797.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001267726.1 Homo sapiens glutaminase 2 (GLS2), transcript variant 4, mRNA.

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CloneID	OHu25114
Clone ID Related Accession (Same CDS sequence)	XM_017019180.1 , NM_001280798.1 , NM_001280797.1
Accession Version	NM_001280797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569254400000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA180204.1, BC025661.1 and AI241400.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 4 which has a shorter N-terminus compared to isoform 1. Both variants 4 and 5 encode the same isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.259298.1, SRR1803616.192907.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCAATG CTGGTGCCAT TGTTGTCAGC TCCCTGATCA AGATGGACTG TAACAAAGCA 
GAGAAGTTTG ATTTTGTGTT GCAGTATCTC AACAAAATGG CTGGGAATGA ATACATGGGT 
TTCAGCAATG CCACATTCCA GTCAGAGAAG GAAACAGGGG ATCGGAATTA TGCCATCGGC 
TATTATCTCA AGGAAAAGAA GTGCTTTCCT AAGGGGGTGG ACATGATGGC TGCCCTTGAT 
CTCTACTTCC AGCTGTGTTC TGTGGAGGTC ACTTGTGAAT CAGGCAGTGT CATGGCAGCC 
ACCCTCGCCA ACGGTGGGAT CTGCCCCATC ACAGGCGAGA GTGTGCTGAG TGCTGAAGCA 
GTGCGCAACA CCCTCAGCCT CATGCATTCC TGCGGCATGT ATGACTTCTC TGGCCAGTTT 
GCCTTCCACG TGGGCCTGCC AGCCAAGTCA GCTGTATCAG GAGCCATCCT CCTGGTGGTA 
CCCAATGTCA TGGGAATGAT GTGCCTGTCA CCCCCATTGG ACAAGCTGGG GAACAGCCAT 
AGGGGGACCA GCTTCTGCCA GAAGTTGGTG TCTCTCTTCA ATTTCCACAA CTATGACAAC 
CTGAGGCACT GTGCTCGGAA GTTAGACCCA CGGCGTGAAG GGGCAGAAAT TCGGAACAAG 
ACTGTGGTCA ACCTGTTATT TGCTGCCTAT AGTGGCGATG TCTCAGCTCT TCGAAGGTTT 
GCCTTGTCAG CCATGGATAT GGAACAGAAA GACTATGACT CGCGCACAGC TCTGCATGTT 
GCTGCAGCTG AAGGACACAT CGAAGTTGTT AAATTCCTGA TCGAGGCTTG CAAAGTGAAT 
CCTTTTGCCA AGGACAGGTG GGGCAACATT CCCCTGGATG ATGCTGTGCA GTTCAACCAT 
CTGGAGGTGG TCAAACTGCT TCAAGATTAC CAGGACTCCT ACACACTCTC TGAAACTCAG 
GCTGAGGCAG CAGCTGAGGC CCTGTCCAAA GAGAACTTAG AAAGCATGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267726.1
CDS	962..1975
Translation	MVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	NM_001280797.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280797.1

ORF Insert Sequence:

ATGGTCAATG CTGGTGCCAT TGTTGTCAGC TCCCTGATCA AGATGGACTG TAACAAAGCA 
GAGAAGTTTG ATTTTGTGTT GCAGTATCTC AACAAAATGG CTGGGAATGA ATACATGGGT 
TTCAGCAATG CCACATTCCA GTCAGAGAAG GAAACAGGGG ATCGGAATTA TGCCATCGGC 
TATTATCTCA AGGAAAAGAA GTGCTTTCCT AAGGGGGTGG ACATGATGGC TGCCCTTGAT 
CTCTACTTCC AGCTGTGTTC TGTGGAGGTC ACTTGTGAAT CAGGCAGTGT CATGGCAGCC 
ACCCTCGCCA ACGGTGGGAT CTGCCCCATC ACAGGCGAGA GTGTGCTGAG TGCTGAAGCA 
GTGCGCAACA CCCTCAGCCT CATGCATTCC TGCGGCATGT ATGACTTCTC TGGCCAGTTT 
GCCTTCCACG TGGGCCTGCC AGCCAAGTCA GCTGTATCAG GAGCCATCCT CCTGGTGGTA 
CCCAATGTCA TGGGAATGAT GTGCCTGTCA CCCCCATTGG ACAAGCTGGG GAACAGCCAT 
AGGGGGACCA GCTTCTGCCA GAAGTTGGTG TCTCTCTTCA ATTTCCACAA CTATGACAAC 
CTGAGGCACT GTGCTCGGAA GTTAGACCCA CGGCGTGAAG GGGCAGAAAT TCGGAACAAG 
ACTGTGGTCA ACCTGTTATT TGCTGCCTAT AGTGGCGATG TCTCAGCTCT TCGAAGGTTT 
GCCTTGTCAG CCATGGATAT GGAACAGAAA GACTATGACT CGCGCACAGC TCTGCATGTT 
GCTGCAGCTG AAGGACACAT CGAAGTTGTT AAATTCCTGA TCGAGGCTTG CAAAGTGAAT 
CCTTTTGCCA AGGACAGGTG GGGCAACATT CCCCTGGATG ATGCTGTGCA GTTCAACCAT 
CTGGAGGTGG TCAAACTGCT TCAAGATTAC CAGGACTCCT ACACACTCTC TGAAACTCAG 
GCTGAGGCAG CAGCTGAGGC CCTGTCCAAA GAGAACTTAG AAAGCATGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLS2 ( XM_017019180.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874669.1 Homo sapiens glutaminase 2 (GLS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu25114
Clone ID Related Accession (Same CDS sequence)	XM_017019180.1 , NM_001280798.1 , NM_001280797.1
Accession Version	XM_017019180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	glutaminase liver isoform, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATG CTGGTGCCAT TGTTGTCAGC TCCCTGATCA AGATGGACTG TAACAAAGCA 
GAGAAGTTTG ATTTTGTGTT GCAGTATCTC AACAAAATGG CTGGGAATGA ATACATGGGT 
TTCAGCAATG CCACATTCCA GTCAGAGAAG GAAACAGGGG ATCGGAATTA TGCCATCGGC 
TATTATCTCA AGGAAAAGAA GTGCTTTCCT AAGGGGGTGG ACATGATGGC TGCCCTTGAT 
CTCTACTTCC AGCTGTGTTC TGTGGAGGTC ACTTGTGAAT CAGGCAGTGT CATGGCAGCC 
ACCCTCGCCA ACGGTGGGAT CTGCCCCATC ACAGGCGAGA GTGTGCTGAG TGCTGAAGCA 
GTGCGCAACA CCCTCAGCCT CATGCATTCC TGCGGCATGT ATGACTTCTC TGGCCAGTTT 
GCCTTCCACG TGGGCCTGCC AGCCAAGTCA GCTGTATCAG GAGCCATCCT CCTGGTGGTA 
CCCAATGTCA TGGGAATGAT GTGCCTGTCA CCCCCATTGG ACAAGCTGGG GAACAGCCAT 
AGGGGGACCA GCTTCTGCCA GAAGTTGGTG TCTCTCTTCA ATTTCCACAA CTATGACAAC 
CTGAGGCACT GTGCTCGGAA GTTAGACCCA CGGCGTGAAG GGGCAGAAAT TCGGAACAAG 
ACTGTGGTCA ACCTGTTATT TGCTGCCTAT AGTGGCGATG TCTCAGCTCT TCGAAGGTTT 
GCCTTGTCAG CCATGGATAT GGAACAGAAA GACTATGACT CGCGCACAGC TCTGCATGTT 
GCTGCAGCTG AAGGACACAT CGAAGTTGTT AAATTCCTGA TCGAGGCTTG CAAAGTGAAT 
CCTTTTGCCA AGGACAGGTG GGGCAACATT CCCCTGGATG ATGCTGTGCA GTTCAACCAT 
CTGGAGGTGG TCAAACTGCT TCAAGATTAC CAGGACTCCT ACACACTCTC TGAAACTCAG 
GCTGAGGCAG CAGCTGAGGC CCTGTCCAAA GAGAACTTAG AAAGCATGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874669.1
CDS	589..1602
Translation	MVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV

Target ORF information:

RefSeq Version	XM_017019180.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminase 2 (GLS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019180.1

ORF Insert Sequence:

ATGGTCAATG CTGGTGCCAT TGTTGTCAGC TCCCTGATCA AGATGGACTG TAACAAAGCA 
GAGAAGTTTG ATTTTGTGTT GCAGTATCTC AACAAAATGG CTGGGAATGA ATACATGGGT 
TTCAGCAATG CCACATTCCA GTCAGAGAAG GAAACAGGGG ATCGGAATTA TGCCATCGGC 
TATTATCTCA AGGAAAAGAA GTGCTTTCCT AAGGGGGTGG ACATGATGGC TGCCCTTGAT 
CTCTACTTCC AGCTGTGTTC TGTGGAGGTC ACTTGTGAAT CAGGCAGTGT CATGGCAGCC 
ACCCTCGCCA ACGGTGGGAT CTGCCCCATC ACAGGCGAGA GTGTGCTGAG TGCTGAAGCA 
GTGCGCAACA CCCTCAGCCT CATGCATTCC TGCGGCATGT ATGACTTCTC TGGCCAGTTT 
GCCTTCCACG TGGGCCTGCC AGCCAAGTCA GCTGTATCAG GAGCCATCCT CCTGGTGGTA 
CCCAATGTCA TGGGAATGAT GTGCCTGTCA CCCCCATTGG ACAAGCTGGG GAACAGCCAT 
AGGGGGACCA GCTTCTGCCA GAAGTTGGTG TCTCTCTTCA ATTTCCACAA CTATGACAAC 
CTGAGGCACT GTGCTCGGAA GTTAGACCCA CGGCGTGAAG GGGCAGAAAT TCGGAACAAG 
ACTGTGGTCA ACCTGTTATT TGCTGCCTAT AGTGGCGATG TCTCAGCTCT TCGAAGGTTT 
GCCTTGTCAG CCATGGATAT GGAACAGAAA GACTATGACT CGCGCACAGC TCTGCATGTT 
GCTGCAGCTG AAGGACACAT CGAAGTTGTT AAATTCCTGA TCGAGGCTTG CAAAGTGAAT 
CCTTTTGCCA AGGACAGGTG GGGCAACATT CCCCTGGATG ATGCTGTGCA GTTCAACCAT 
CTGGAGGTGG TCAAACTGCT TCAAGATTAC CAGGACTCCT ACACACTCTC TGAAACTCAG 
GCTGAGGCAG CAGCTGAGGC CCTGTCCAAA GAGAACTTAG AAAGCATGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human genetic variation in GLS2 is associated with development of complicated Staphylococcus aureus bacteremia.
PLoS genetics14(10)e1007667(2018 10)
Scott WK,Medie FM,Ruffin F,Sharma-Kuinkel BK,Cyr DD,Guo S,Dykxhoorn DM,Skov RL,Bruun NE,Dahl A,Lerche CJ,Petersen A,Larsen AR,Lauridsen TK,Johansen HK,Ullum H,Sørensen E,Hassager C,Bundgaard H,Schønheyder HC,Torp-Pedersen C,Østergaard LB,Arpi M,Rosenvinge F,Erikstrup LT,Chehri M,Søgaard P,Andersen PS,Fowler VG Jr

The origin and evolution of human glutaminases and their atypical C-terminal ankyrin repeats.
The Journal of biological chemistry292(27)11572-11585(2017 07)
Pasquali CC,Islam Z,Adamoski D,Ferreira IM,Righeto RD,Bettini J,Portugal RV,Yue WW,Gonzalez A,Dias SMG,Ambrosio ALB

Glutaminase 2 stabilizes Dicer to repress Snail and metastasis in hepatocellular carcinoma cells.
Cancer letters383(2)282-294(2016 12)
Kuo TC,Chen CK,Hua KT,Yu P,Lee WJ,Chen MW,Jeng YM,Chien MH,Kuo KT,Hsiao M,Kuo ML

CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.
RNA (New York, N.Y.)22(6)830-8(2016 06)
Masamha CP,Xia Z,Peart N,Collum S,Li W,Wagner EJ,Shyu AB

The combination of three molecular markers can be a valuable predictive tool for the prognosis of hepatocellular carcinoma patients.
Scientific reports624582(2016 Apr)
Chen SS,Yu KK,Ling QX,Huang C,Li N,Zheng JM,Bao SX,Cheng Q,Zhu MQ,Chen MQ

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GLS2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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