MTHFD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFD2
Entrez Gene ID	10797
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Synonyms	NMDMC
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase Names bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.1
Summary	This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009].
Disorder MIM:	604887

mRNA and Protein(s)

mRNA	Protein	Name
XM_006711924.2	XP_006711987.1	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial isoform X1
NM_006636.3	NP_006627.2	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial precursor
NM_006636.4	NP_006627.2	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MTHFD2	XP_005630608.1
Gallus gallus (chicken)	MTHFD2	NP_001026531.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004677	XP_001687783.1
Danio rerio (zebrafish)	LOC101886265	XP_005168726.1
Pan troglodytes (chimpanzee)	MTHFD2	XP_001154612.1
Bos taurus (cattle)	MTHFD2	NP_001069223.1
Mus musculus (house mouse)	Mthfd2	NP_032664.1
Xenopus tropicalis (tropical clawed frog)	mthfd2	NP_001123716.1
Rattus norvegicus (Norway rat)	Mthfd2	NP_001102868.1
Homo sapiens (human)	MTHFD2	NP_006627.2
Drosophila melanogaster (fruit fly)	Nmdmc	NP_476929.1
Danio rerio (zebrafish)	mthfd2	NP_001002181.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial MTHFD isozymes display distinct expression, regulation, and association with cancer.
Nilsson R, Nicolaidou V, Koufaris C
Gene716144032(2019 Oct)

Cancer stem-like properties and gefitinib resistance are dependent on purine synthetic metabolism mediated by the mitochondrial enzyme MTHFD2.
Nishimura T, Nakata A, Chen X, Nishi K, Meguro-Horike M, Sasaki S, Kita K, Horike SI, Saitoh K, Kato K, Igarashi K, Murayama T, Kohno S, Takahashi C, Mukaida N, Yano S, Soga T, Tojo A, Gotoh N
Oncogene38(14)2464-2481(2019 04)

Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells.
Hitzel J, Lee E, Zhang Y, Bibli SI, Li X, Zukunft S, Pflüger B, Hu J, Schürmann C, Vasconez AE, Oo JA, Kratzer A, Kumar S, Rezende F, Josipovic I, Thomas D, Giral H, Schreiber Y, Geisslinger G, Fork C, Yang X, Sigala F, Romanoski CE, Kroll J, Jo H, Landmesser U, Lusis AJ, Namgaladze D, Fleming I, Leisegang MS, Zhu J, Brandes RP
Nature communications9(1)2292(2018 06)

MTHFD2 Overexpression Predicts Poor Prognosis in Renal Cell Carcinoma and is Associated with Cell Proliferation and Vimentin-Modulated Migration and Invasion.
Lin H, Huang B, Wang H, Liu X, Hong Y, Qiu S, Zheng J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology51(2)991-1000(2018)

miR-92a Inhibits Proliferation and Induces Apoptosis by Regulating Methylenetetrahydrofolate Dehydrogenase 2 (MTHFD2) Expression in Acute Myeloid Leukemia.
Gu Y, Si J, Xiao X, Tian Y, Yang S
Oncology research25(7)1069-1079(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTHFD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18706	NM_006636.4 Latest version!	Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$144.50-$202.30 $289.00
OHu35257	XM_006711924.2 Latest version!	Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu18706	NM_006636.3	Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$144.50-$202.30 $289.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFD2 ( NM_006636.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006627.2 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18706
Clone ID Related Accession (Same CDS sequence)	NM_006636.3 , NM_006636.4
Accession Version	NM_006636.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512403200000
Organism	Homo sapiens(human)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX475613.1, BC017054.1 and CB124593.1. This sequence is a reference standard in the RefSeqGene project. On May 5, 2006 this sequence version replaced NM_006636.2. Transcript Variant: This variant (1) represents the shorter transcript and is the protein-coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.199777.1, SRR1803615.72638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGA CTTCTCTAAT GTCTGCTTTG GCTGCCCGGC TGCTGCAGCC CGCGCACAGC 
TGCTCCCTTC GCCTTCGCCC TTTCCACCTC GCGGCAGTTC GAAATGAAGC TGTTGTCATT 
TCTGGAAGGA AACTGGCCCA GCAGATCAAG CAGGAAGTGC GGCAGGAGGT AGAAGAGTGG 
GTGGCCTCAG GCAACAAACG GCCACACCTG AGTGTGATCC TGGTTGGCGA GAATCCTGCA 
AGTCACTCCT ATGTCCTCAA CAAAACCAGG GCAGCTGCAG TTGTGGGAAT CAACAGTGAG 
ACAATTATGA AACCAGCTTC AATTTCAGAG GAAGAATTGT TGAATTTAAT CAATAAACTG 
AATAATGATG ATAATGTAGA TGGCCTCCTT GTTCAGTTGC CTCTTCCAGA GCATATTGAT 
GAGAGAAGGA TCTGCAATGC TGTTTCTCCA GACAAGGATG TTGATGGCTT TCATGTAATT 
AATGTAGGAC GAATGTGTTT GGATCAGTAT TCCATGTTAC CGGCTACTCC ATGGGGTGTG 
TGGGAAATAA TCAAGCGAAC TGGCATTCCA ACCCTAGGGA AGAATGTGGT TGTGGCTGGA 
AGGTCAAAAA ACGTTGGAAT GCCCATTGCA ATGTTACTGC ACACAGATGG GGCGCATGAA 
CGTCCCGGAG GTGATGCCAC TGTTACAATA TCTCATCGAT ATACTCCCAA AGAGCAGTTG 
AAGAAACATA CAATTCTTGC AGATATTGTA ATATCTGCTG CAGGTATTCC AAATCTGATC 
ACAGCAGATA TGATCAAGGA AGGAGCAGCA GTCATTGATG TGGGAATAAA TAGAGTTCAC 
GATCCTGTAA CTGCCAAACC CAAGTTGGTT GGAGATGTGG ATTTTGAAGG AGTCAGACAA 
AAAGCTGGGT ATATCACTCC AGTTCCTGGA GGTGTTGGCC CCATGACAGT GGCAATGCTA 
ATGAAGAATA CCATTATTGC TGCAAAAAAG GTGCTGAGGC TTGAAGAGCG AGAAGTGCTG 
AAGTCTAAAG AGCTTGGGGT AGCCACTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006627.2
CDS	80..1132
Translation	MAATSLMSALAARLLQPAHSCSLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN

Target ORF information:

RefSeq Version	NM_006636.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006636.3

ORF Insert Sequence:

ATGGCTGCGA CTTCTCTAAT GTCTGCTTTG GCTGCCCGGC TGCTGCAGCC CGCGCACAGC 
TGCTCCCTTC GCCTTCGCCC TTTCCACCTC GCGGCAGTTC GAAATGAAGC TGTTGTCATT 
TCTGGAAGGA AACTGGCCCA GCAGATCAAG CAGGAAGTGC GGCAGGAGGT AGAAGAGTGG 
GTGGCCTCAG GCAACAAACG GCCACACCTG AGTGTGATCC TGGTTGGCGA GAATCCTGCA 
AGTCACTCCT ATGTCCTCAA CAAAACCAGG GCAGCTGCAG TTGTGGGAAT CAACAGTGAG 
ACAATTATGA AACCAGCTTC AATTTCAGAG GAAGAATTGT TGAATTTAAT CAATAAACTG 
AATAATGATG ATAATGTAGA TGGCCTCCTT GTTCAGTTGC CTCTTCCAGA GCATATTGAT 
GAGAGAAGGA TCTGCAATGC TGTTTCTCCA GACAAGGATG TTGATGGCTT TCATGTAATT 
AATGTAGGAC GAATGTGTTT GGATCAGTAT TCCATGTTAC CGGCTACTCC ATGGGGTGTG 
TGGGAAATAA TCAAGCGAAC TGGCATTCCA ACCCTAGGGA AGAATGTGGT TGTGGCTGGA 
AGGTCAAAAA ACGTTGGAAT GCCCATTGCA ATGTTACTGC ACACAGATGG GGCGCATGAA 
CGTCCCGGAG GTGATGCCAC TGTTACAATA TCTCATCGAT ATACTCCCAA AGAGCAGTTG 
AAGAAACATA CAATTCTTGC AGATATTGTA ATATCTGCTG CAGGTATTCC AAATCTGATC 
ACAGCAGATA TGATCAAGGA AGGAGCAGCA GTCATTGATG TGGGAATAAA TAGAGTTCAC 
GATCCTGTAA CTGCCAAACC CAAGTTGGTT GGAGATGTGG ATTTTGAAGG AGTCAGACAA 
AAAGCTGGGT ATATCACTCC AGTTCCTGGA GGTGTTGGCC CCATGACAGT GGCAATGCTA 
ATGAAGAATA CCATTATTGC TGCAAAAAAG GTGCTGAGGC TTGAAGAGCG AGAAGTGCTG 
AAGTCTAAAG AGCTTGGGGT AGCCACTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD2 ( NM_006636.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006627.2 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18706
Clone ID Related Accession (Same CDS sequence)	NM_006636.3 , NM_006636.4
Accession Version	NM_006636.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Homo sapiens(human)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX475613.1, BC017054.1, CB124593.1 and AC073263.5. On Nov 23, 2018 this sequence version replaced NM_006636.3. Transcript Variant: This variant (1) represents the shorter transcript and is the protein-coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130664.1, SRR1803612.261966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000394053.7/ ENSP00000377617.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCGA CTTCTCTAAT GTCTGCTTTG GCTGCCCGGC TGCTGCAGCC CGCGCACAGC 
TGCTCCCTTC GCCTTCGCCC TTTCCACCTC GCGGCAGTTC GAAATGAAGC TGTTGTCATT 
TCTGGAAGGA AACTGGCCCA GCAGATCAAG CAGGAAGTGC GGCAGGAGGT AGAAGAGTGG 
GTGGCCTCAG GCAACAAACG GCCACACCTG AGTGTGATCC TGGTTGGCGA GAATCCTGCA 
AGTCACTCCT ATGTCCTCAA CAAAACCAGG GCAGCTGCAG TTGTGGGAAT CAACAGTGAG 
ACAATTATGA AACCAGCTTC AATTTCAGAG GAAGAATTGT TGAATTTAAT CAATAAACTG 
AATAATGATG ATAATGTAGA TGGCCTCCTT GTTCAGTTGC CTCTTCCAGA GCATATTGAT 
GAGAGAAGGA TCTGCAATGC TGTTTCTCCA GACAAGGATG TTGATGGCTT TCATGTAATT 
AATGTAGGAC GAATGTGTTT GGATCAGTAT TCCATGTTAC CGGCTACTCC ATGGGGTGTG 
TGGGAAATAA TCAAGCGAAC TGGCATTCCA ACCCTAGGGA AGAATGTGGT TGTGGCTGGA 
AGGTCAAAAA ACGTTGGAAT GCCCATTGCA ATGTTACTGC ACACAGATGG GGCGCATGAA 
CGTCCCGGAG GTGATGCCAC TGTTACAATA TCTCATCGAT ATACTCCCAA AGAGCAGTTG 
AAGAAACATA CAATTCTTGC AGATATTGTA ATATCTGCTG CAGGTATTCC AAATCTGATC 
ACAGCAGATA TGATCAAGGA AGGAGCAGCA GTCATTGATG TGGGAATAAA TAGAGTTCAC 
GATCCTGTAA CTGCCAAACC CAAGTTGGTT GGAGATGTGG ATTTTGAAGG AGTCAGACAA 
AAAGCTGGGT ATATCACTCC AGTTCCTGGA GGTGTTGGCC CCATGACAGT GGCAATGCTA 
ATGAAGAATA CCATTATTGC TGCAAAAAAG GTGCTGAGGC TTGAAGAGCG AGAAGTGCTG 
AAGTCTAAAG AGCTTGGGGT AGCCACTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006627.2
CDS	28..1080
Translation	MAATSLMSALAARLLQPAHSCSLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN

Target ORF information:

RefSeq Version	NM_006636.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006636.4

ORF Insert Sequence:

ATGGCTGCGA CTTCTCTAAT GTCTGCTTTG GCTGCCCGGC TGCTGCAGCC CGCGCACAGC 
TGCTCCCTTC GCCTTCGCCC TTTCCACCTC GCGGCAGTTC GAAATGAAGC TGTTGTCATT 
TCTGGAAGGA AACTGGCCCA GCAGATCAAG CAGGAAGTGC GGCAGGAGGT AGAAGAGTGG 
GTGGCCTCAG GCAACAAACG GCCACACCTG AGTGTGATCC TGGTTGGCGA GAATCCTGCA 
AGTCACTCCT ATGTCCTCAA CAAAACCAGG GCAGCTGCAG TTGTGGGAAT CAACAGTGAG 
ACAATTATGA AACCAGCTTC AATTTCAGAG GAAGAATTGT TGAATTTAAT CAATAAACTG 
AATAATGATG ATAATGTAGA TGGCCTCCTT GTTCAGTTGC CTCTTCCAGA GCATATTGAT 
GAGAGAAGGA TCTGCAATGC TGTTTCTCCA GACAAGGATG TTGATGGCTT TCATGTAATT 
AATGTAGGAC GAATGTGTTT GGATCAGTAT TCCATGTTAC CGGCTACTCC ATGGGGTGTG 
TGGGAAATAA TCAAGCGAAC TGGCATTCCA ACCCTAGGGA AGAATGTGGT TGTGGCTGGA 
AGGTCAAAAA ACGTTGGAAT GCCCATTGCA ATGTTACTGC ACACAGATGG GGCGCATGAA 
CGTCCCGGAG GTGATGCCAC TGTTACAATA TCTCATCGAT ATACTCCCAA AGAGCAGTTG 
AAGAAACATA CAATTCTTGC AGATATTGTA ATATCTGCTG CAGGTATTCC AAATCTGATC 
ACAGCAGATA TGATCAAGGA AGGAGCAGCA GTCATTGATG TGGGAATAAA TAGAGTTCAC 
GATCCTGTAA CTGCCAAACC CAAGTTGGTT GGAGATGTGG ATTTTGAAGG AGTCAGACAA 
AAAGCTGGGT ATATCACTCC AGTTCCTGGA GGTGTTGGCC CCATGACAGT GGCAATGCTA 
ATGAAGAATA CCATTATTGC TGCAAAAAAG GTGCTGAGGC TTGAAGAGCG AGAAGTGCTG 
AAGTCTAAAG AGCTTGGGGT AGCCACTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD2 ( XM_006711924.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711987.1 Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35257
Clone ID Related Accession (Same CDS sequence)	XM_006711924.2
Accession Version	XM_006711924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006711924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG CTTCAATTTC AGAGGAAGAA TTGTTGAATT TAATCAATAA ACTGAATAAT 
GATGATAATG TAGATGGCCT CCTTGTTCAG TTGCCTCTTC CAGAGCATAT TGATGAGAGA 
AGGATCTGCA ATGCTGTTTC TCCAGACAAG GATGTTGATG GCTTTCATGT AATTAATGTA 
GGACGAATGT GTTTGGATCA GTATTCCATG TTACCGGCTA CTCCATGGGG TGTGTGGGAA 
ATAATCAAGC GAACTGGCAT TCCAACCCTA GGGAAGAATG TGGTTGTGGC TGGAAGGTCA 
AAAAACGTTG GAATGCCCAT TGCAATGTTA CTGCACACAG ATGGGGCGCA TGAACGTCCC 
GGAGGTGATG CCACTGTTAC AATATCTCAT CGATATACTC CCAAAGAGCA GTTGAAGAAA 
CATACAATTC TTGCAGATAT TGTAATATCT GCTGCAGGTA TTCCAAATCT GATCACAGCA 
GATATGATCA AGGAAGGAGC AGCAGTCATT GATGTGGGAA TAAATAGAGT TCACGATCCT 
GTAACTGCCA AACCCAAGTT GGTTGGAGAT GTGGATTTTG AAGGAGTCAG ACAAAAAGCT 
GGGTATATCA CTCCAGTTCC TGGAGGTGTT GGCCCCATGA CAGTGGCAAT GCTAATGAAG 
AATACCATTA TTGCTGCAAA AAAGGTGCTG AGGCTTGAAG AGCGAGAAGT GCTGAAGTCT 
AAAGAGCTTG GGGTAGCCAC TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711987.1
CDS	173..919
Translation	MKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN

Target ORF information:

RefSeq Version	XM_006711924.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711924.2

ORF Insert Sequence:

ATGAAACCAG CTTCAATTTC AGAGGAAGAA TTGTTGAATT TAATCAATAA ACTGAATAAT 
GATGATAATG TAGATGGCCT CCTTGTTCAG TTGCCTCTTC CAGAGCATAT TGATGAGAGA 
AGGATCTGCA ATGCTGTTTC TCCAGACAAG GATGTTGATG GCTTTCATGT AATTAATGTA 
GGACGAATGT GTTTGGATCA GTATTCCATG TTACCGGCTA CTCCATGGGG TGTGTGGGAA 
ATAATCAAGC GAACTGGCAT TCCAACCCTA GGGAAGAATG TGGTTGTGGC TGGAAGGTCA 
AAAAACGTTG GAATGCCCAT TGCAATGTTA CTGCACACAG ATGGGGCGCA TGAACGTCCC 
GGAGGTGATG CCACTGTTAC AATATCTCAT CGATATACTC CCAAAGAGCA GTTGAAGAAA 
CATACAATTC TTGCAGATAT TGTAATATCT GCTGCAGGTA TTCCAAATCT GATCACAGCA 
GATATGATCA AGGAAGGAGC AGCAGTCATT GATGTGGGAA TAAATAGAGT TCACGATCCT 
GTAACTGCCA AACCCAAGTT GGTTGGAGAT GTGGATTTTG AAGGAGTCAG ACAAAAAGCT 
GGGTATATCA CTCCAGTTCC TGGAGGTGTT GGCCCCATGA CAGTGGCAAT GCTAATGAAG 
AATACCATTA TTGCTGCAAA AAAGGTGCTG AGGCTTGAAG AGCGAGAAGT GCTGAAGTCT 
AAAGAGCTTG GGGTAGCCAC TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial MTHFD isozymes display distinct expression, regulation, and association with cancer.
Gene716144032(2019 Oct)
Nilsson R,Nicolaidou V,Koufaris C

Cancer stem-like properties and gefitinib resistance are dependent on purine synthetic metabolism mediated by the mitochondrial enzyme MTHFD2.
Oncogene38(14)2464-2481(2019 04)
Nishimura T,Nakata A,Chen X,Nishi K,Meguro-Horike M,Sasaki S,Kita K,Horike SI,Saitoh K,Kato K,Igarashi K,Murayama T,Kohno S,Takahashi C,Mukaida N,Yano S,Soga T,Tojo A,Gotoh N

Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells.
Nature communications9(1)2292(2018 06)
Hitzel J,Lee E,Zhang Y,Bibli SI,Li X,Zukunft S,Pflüger B,Hu J,Schürmann C,Vasconez AE,Oo JA,Kratzer A,Kumar S,Rezende F,Josipovic I,Thomas D,Giral H,Schreiber Y,Geisslinger G,Fork C,Yang X,Sigala F,Romanoski CE,Kroll J,Jo H,Landmesser U,Lusis AJ,Namgaladze D,Fleming I,Leisegang MS,Zhu J,Brandes RP

MTHFD2 Overexpression Predicts Poor Prognosis in Renal Cell Carcinoma and is Associated with Cell Proliferation and Vimentin-Modulated Migration and Invasion.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology51(2)991-1000(2018)
Lin H,Huang B,Wang H,Liu X,Hong Y,Qiu S,Zheng J

miR-92a Inhibits Proliferation and Induces Apoptosis by Regulating Methylenetetrahydrofolate Dehydrogenase 2 (MTHFD2) Expression in Acute Myeloid Leukemia.
Oncology research25(7)1069-1079(2017 Aug)
Gu Y,Si J,Xiao X,Tian Y,Yang S

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