Mthfd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mthfd2
Entrez Gene ID	680308
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase Names bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase methylenetetrahydrofolate dehydrogenase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592887.1	XP_017448376.1	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial isoform X1
NM_001109398.1	NP_001102868.1	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MTHFD2	XP_005630608.1
Gallus gallus (chicken)	MTHFD2	NP_001026531.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004677	XP_001687783.1
Danio rerio (zebrafish)	LOC101886265	XP_005168726.1
Pan troglodytes (chimpanzee)	MTHFD2	XP_001154612.1
Bos taurus (cattle)	MTHFD2	NP_001069223.1
Mus musculus (house mouse)	Mthfd2	NP_032664.1
Xenopus tropicalis (tropical clawed frog)	mthfd2	NP_001123716.1
Rattus norvegicus (Norway rat)	Mthfd2	NP_001102868.1
Homo sapiens (human)	MTHFD2	NP_006627.2
Drosophila melanogaster (fruit fly)	Nmdmc	NP_476929.1
Danio rerio (zebrafish)	mthfd2	NP_001002181.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Böhm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, Kölbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Update and new concepts in vitamin responsive disorders of folate transport and metabolism.
Watkins D, Rosenblatt DS
Journal of inherited metabolic disease35(4)665-70(2012 Jul)

Compartmentalization of Mammalian folate-mediated one-carbon metabolism.
Tibbetts AS, Appling DR
Annual review of nutrition3057-81(2010 Aug)

Transpupillary thermotherapy-induced modification of angiogenesis- and coagulation-related gene expression in the rat posterior fundus.
Ito YN, Ito M, Takita H, Yoneya S, Peyman GA, Gehlbach PL, Mori K
Molecular vision12802-10(2006 Jul)

Magnesium and phosphate ions enable NAD binding to methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase.
Christensen KE, Mirza IA, Berghuis AM, Mackenzie RE
The Journal of biological chemistry280(40)34316-23(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mthfd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mthfd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09819	NM_001109398.1 Latest version!	Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa61010	XM_017592887.1 Latest version!	Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mthfd2 ( NM_001109398.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102868.1 Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2), mRNA.

Price & Availability↑

CloneID	ORa09819
Clone ID Related Accession (Same CDS sequence)	NM_001109398.1
Accession Version	NM_001109398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473957.1. On or before Oct 4, 2007 this sequence version replaced XM_001056154.1, XM_001056213.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMN01906349 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGTTTCTG TTTCCTTGTC TGCGCTGGCA GTGCGGTTGT TGCGCCCCAC GCACGGCTGC 
CACTCCCGCC TACAGTCCTT CCACCTGGCG GCAGTACGAA ATGAAGCCGT GGTCATTTCT 
GGAAGGAAGC TGGCCCAGCA GATTAAGCAA GAAGTACGGC AGGAGGTGGA AGAGTGGGTG 
GCCTCGGGCA ACAAGCGGCC ACACCTCAGT GTCATTCTGG TTGGCGACAA TCCTGCCAGT 
CACTCCTATG TTCTCAACAA AACCAGGGCA GCAGCTGAAG TGGGAATCAA CAGTGAGACA 
ATTGTGAAAC CAGCCTCAGT GTCAGAGGAA GAGCTGTTGA ATTCAATCAG GACATTGAAT 
GACGATGAGA ATGTAGATGG CCTCCTTGTT CAGCTGCCAC TCCCAGAGCA CATTGACGAG 
AGAAAGATCT GCAATGCTGT TTCTCCTGAC AAGGATGTTG ATGGCTTTCA TGTCATCAAC 
GTTGGGCGCA TGTGTTTGGA CCAGTATTCC ATGCTGCCAG CAACTCCATG GGGCGTGTGG 
GAGATACTCA AGCGAACAGG CATTCCAACC TTAGGGAAGA ATGTGGTAGT GGCTGGCAGG 
TCCAAAAACG TTGGGATGCC AATTGCAATG CTGCTGCACA CGGATGGGGC ACACGAGCGG 
CCTGGGGGTG ATGCCACAGT CACAATATCT CATCGGCACA CTCCCAAAGA ACAGCTGAAG 
AAGCACACGA TCCTTGCGGA CATTGTGATA TCTGCTGCTG GCATTCCAAA CCTCATCACA 
GCTGACATGA TCAAGGAAGG AGCAACAGTC ATCGACGTGG GGATAAACAG AGTTCAAGAT 
CCTGTCACCG CAAAGCCCAA GTTGGTTGGA GATGTGGATT TTGAAGGAGT CAAGAAGAAA 
GCTGGTTACA TCACTCCTGT CCCTGGGGGT GTTGGTCCCA TGACAGTGGC CATGCTCATG 
AAGAACACCA TTATTGCTGC AAAGAAAGTG CTCAGGCCAG AGGAGCTGGA AGTGTTGAAG 
TCCACACAGC GCGGAGTTGC CACCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102868.1
CDS	76..1125
Translation	MVSVSLSALAVRLLRPTHGCHSRLQSFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGDNPASHSYVLNKTRAAAEVGINSETIVKPASVSEEELLNSIRTLNDDENVDGLLVQLPLPEHIDERKICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEILKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRHTPKEQLKKHTILADIVISAAGIPNLITADMIKEGATVIDVGINRVQDPVTAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRPEELEVLKSTQRGVATN

Target ORF information:

RefSeq Version	NM_001109398.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109398.1

ORF Insert Sequence:

ATGGTTTCTG TTTCCTTGTC TGCGCTGGCA GTGCGGTTGT TGCGCCCCAC GCACGGCTGC 
CACTCCCGCC TACAGTCCTT CCACCTGGCG GCAGTACGAA ATGAAGCCGT GGTCATTTCT 
GGAAGGAAGC TGGCCCAGCA GATTAAGCAA GAAGTACGGC AGGAGGTGGA AGAGTGGGTG 
GCCTCGGGCA ACAAGCGGCC ACACCTCAGT GTCATTCTGG TTGGCGACAA TCCTGCCAGT 
CACTCCTATG TTCTCAACAA AACCAGGGCA GCAGCTGAAG TGGGAATCAA CAGTGAGACA 
ATTGTGAAAC CAGCCTCAGT GTCAGAGGAA GAGCTGTTGA ATTCAATCAG GACATTGAAT 
GACGATGAGA ATGTAGATGG CCTCCTTGTT CAGCTGCCAC TCCCAGAGCA CATTGACGAG 
AGAAAGATCT GCAATGCTGT TTCTCCTGAC AAGGATGTTG ATGGCTTTCA TGTCATCAAC 
GTTGGGCGCA TGTGTTTGGA CCAGTATTCC ATGCTGCCAG CAACTCCATG GGGCGTGTGG 
GAGATACTCA AGCGAACAGG CATTCCAACC TTAGGGAAGA ATGTGGTAGT GGCTGGCAGG 
TCCAAAAACG TTGGGATGCC AATTGCAATG CTGCTGCACA CGGATGGGGC ACACGAGCGG 
CCTGGGGGTG ATGCCACAGT CACAATATCT CATCGGCACA CTCCCAAAGA ACAGCTGAAG 
AAGCACACGA TCCTTGCGGA CATTGTGATA TCTGCTGCTG GCATTCCAAA CCTCATCACA 
GCTGACATGA TCAAGGAAGG AGCAACAGTC ATCGACGTGG GGATAAACAG AGTTCAAGAT 
CCTGTCACCG CAAAGCCCAA GTTGGTTGGA GATGTGGATT TTGAAGGAGT CAAGAAGAAA 
GCTGGTTACA TCACTCCTGT CCCTGGGGGT GTTGGTCCCA TGACAGTGGC CATGCTCATG 
AAGAACACCA TTATTGCTGC AAAGAAAGTG CTCAGGCCAG AGGAGCTGGA AGTGTTGAAG 
TCCACACAGC GCGGAGTTGC CACCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfd2 ( XM_017592887.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448376.1 Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61010
Clone ID Related Accession (Same CDS sequence)	XM_017592887.1
Accession Version	XM_017592887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCTG TTTCCTTGTC TGCGCTGGCA GTGCGGTTGT TGCGCCCCAC GCACGGCTGC 
CACTCCCGCC TACAGTCCTT CCACCTGGCG GCAGTACGAA ATGAAGCCGT GGTCATTTCT 
GGAAGGAAGC TGGCCCAGCA GATTAAGCAA GAAGTACGGC AGGAGGTGGA AGAGTGGGTG 
GCCTCGGGCA ACAAGCGGCC ACACCTCAGT GTCATTCTGG TTGGCGACAA TCCTGCCAGT 
CACTCCTATG TTCTCAACAA AACCAGGGCA GCAGCTGAAG TGGGAATCAA CAGTGAGACA 
ATTGTGAAAC CAGCCTCAGT GTCAGAGGAA GAGCTGTTGA ATTCAATCAG GACATTGAAT 
GACGATGAGA ATGTAGATGG CCTCCTTGTT CAGCTGCCAC TCCCAGAGCA CATTGACGAG 
AGAAAGATCT GCAATGCTGT TTCTCCTGAC AAGGATGTTG ATGGCTTTCA TGTCATCAAC 
GTTGGGCGCA TGTGTTTGGA CCAGTATTCC ATGCTGCCAG CAACTCCATG GGGCGTGTGG 
GAGATACTCA AGCGAACAGG TGATGCCACA GTCACAATAT CTCATCGGCA CACTCCCAAA 
GAACAGCTGA AGAAGCACAC GATCCTTGCG GACATTGTGA TATCTGCTGC TGGCATTCCA 
AACCTCATCA CAGCTGACAT GATCAAGGAA GGAGCAACAG TCATCGACGT GGGGATAAAC 
AGAGTTCAAG ATCCTGTCAC CGCAAAGCCC AAGTTGGTTG GAGATGTGGA TTTTGAAGGA 
GTCAAGAAGA AAGCTGGTTA CATCACTCCT GTCCCTGGGG GTGTTGGTCC CATGACAGTG 
GCCATGCTCA TGAAGAACAC CATTATTGCT GCAAAGAAAG TGCTCAGGCC AGAGGAGCTG 
GAAGTGTTGA AGTCCACACA GCGCGGAGTT GCCACCAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448376.1
CDS	51..992
Translation	MVSVSLSALAVRLLRPTHGCHSRLQSFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGDNPASHSYVLNKTRAAAEVGINSETIVKPASVSEEELLNSIRTLNDDENVDGLLVQLPLPEHIDERKICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEILKRTGDATVTISHRHTPKEQLKKHTILADIVISAAGIPNLITADMIKEGATVIDVGINRVQDPVTAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRPEELEVLKSTQRGVATN

Target ORF information:

RefSeq Version	XM_017592887.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592887.1

ORF Insert Sequence:

ATGGTTTCTG TTTCCTTGTC TGCGCTGGCA GTGCGGTTGT TGCGCCCCAC GCACGGCTGC 
CACTCCCGCC TACAGTCCTT CCACCTGGCG GCAGTACGAA ATGAAGCCGT GGTCATTTCT 
GGAAGGAAGC TGGCCCAGCA GATTAAGCAA GAAGTACGGC AGGAGGTGGA AGAGTGGGTG 
GCCTCGGGCA ACAAGCGGCC ACACCTCAGT GTCATTCTGG TTGGCGACAA TCCTGCCAGT 
CACTCCTATG TTCTCAACAA AACCAGGGCA GCAGCTGAAG TGGGAATCAA CAGTGAGACA 
ATTGTGAAAC CAGCCTCAGT GTCAGAGGAA GAGCTGTTGA ATTCAATCAG GACATTGAAT 
GACGATGAGA ATGTAGATGG CCTCCTTGTT CAGCTGCCAC TCCCAGAGCA CATTGACGAG 
AGAAAGATCT GCAATGCTGT TTCTCCTGAC AAGGATGTTG ATGGCTTTCA TGTCATCAAC 
GTTGGGCGCA TGTGTTTGGA CCAGTATTCC ATGCTGCCAG CAACTCCATG GGGCGTGTGG 
GAGATACTCA AGCGAACAGG TGATGCCACA GTCACAATAT CTCATCGGCA CACTCCCAAA 
GAACAGCTGA AGAAGCACAC GATCCTTGCG GACATTGTGA TATCTGCTGC TGGCATTCCA 
AACCTCATCA CAGCTGACAT GATCAAGGAA GGAGCAACAG TCATCGACGT GGGGATAAAC 
AGAGTTCAAG ATCCTGTCAC CGCAAAGCCC AAGTTGGTTG GAGATGTGGA TTTTGAAGGA 
GTCAAGAAGA AAGCTGGTTA CATCACTCCT GTCCCTGGGG GTGTTGGTCC CATGACAGTG 
GCCATGCTCA TGAAGAACAC CATTATTGCT GCAAAGAAAG TGCTCAGGCC AGAGGAGCTG 
GAAGTGTTGA AGTCCACACA GCGCGGAGTT GCCACCAACT AG