PTCH1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTCH1
Entrez Gene ID	395806
Full Name	patched 1
Synonyms	PTC,PTC1,PTCH
General protein information	Preferred Names patched 1 Names protein patched homolog 1 patched homolog 1 patched protein patched-like protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015280248.2	XP_015135734.1	protein patched homolog 1 isoform X1
NM_204960.2	NP_990291.2	protein patched homolog 1
XM_025144670.1	XP_025000438.1	protein patched homolog 1 isoform X2
XM_015280248.1	XP_015135734.1	protein patched homolog 1 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PTCH1	XP_001111868.2
Canis lupus familiaris (dog)	PTCH1	XP_003638919.1
Rattus norvegicus (Norway rat)	Ptch1	NP_446018.1
Gallus gallus (chicken)	PTCH1	NP_990291.1
Caenorhabditis elegans (roundworm)	ptc-1	NP_495662.2
Danio rerio (zebrafish)	ptch1	XP_001922161.4
Caenorhabditis elegans (roundworm)	ptc-3	NP_494384.2
Xenopus tropicalis (tropical clawed frog)	ptch1	XP_002935019.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011395	XP_317925.4
Pan troglodytes (chimpanzee)	PTCH1	XP_003312241.1
Bos taurus (cattle)	PTCH1	NP_001192808.1
Caenorhabditis elegans (roundworm)	ptc-2	NP_495218.2
Mus musculus (house mouse)	Ptch1	NP_032983.1
Homo sapiens (human)	PTCH1	NP_000255.2
Drosophila melanogaster (fruit fly)	ptc	NP_523661.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, Bouneau L, Fischer C, Ozouf-Costaz C, Bernot A, Nicaud S, Jaffe D, Fisher S, Lutfalla G, Dossat C, Segurens B, Dasilva C, Salanoubat M, Levy M, Boudet N, Castellano S, Anthouard V, Jubin C, Castelli V, Katinka M, Vacherie B, Biémont C, Skalli Z, Cattolico L, Poulain J, De Berardinis V, Cruaud C, Duprat S, Brottier P, Coutanceau JP, Gouzy J, Parra G, Lardier G, Chapple C, McKernan KJ, McEwan P, Bosak S, Kellis M, Volff JN, Guigó R, Zody MC, Mesirov J, Lindblad-Toh K, Birren B, Nusbaum C, Kahn D, Robinson-Rechavi M, Laudet V, Schachter V, Quétier F, Saurin W, Scarpelli C, Wincker P, Lander ES, Weissenbach J, Roest Crollius H
Nature431(7011)946-57(2004 Oct)

Male-biased mutation rate and divergence in autosomal, z-linked and w-linked introns of chicken and Turkey.
Axelsson E, Smith NG, Sundström H, Berlin S, Ellegren H
Molecular biology and evolution21(8)1538-47(2004 Aug)

Biochemical evidence that patched is the Hedgehog receptor.
Marigo V, Davey RA, Zuo Y, Cunningham JM, Tabin CJ
Nature384(6605)176-9(1996 Nov)

Conservation in hedgehog signaling: induction of a chicken patched homolog by Sonic hedgehog in the developing limb.
Marigo V, Scott MP, Johnson RL, Goodrich LV, Tabin CJ
Development (Cambridge, England)122(4)1225-33(1996 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa28384	NM_204960.2 Latest version!	Gallus gallus patched 1 (PTCH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa152959	XM_025144670.1 Latest version!	Gallus gallus patched 1 (PTCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa48039	XM_015280248.2 Latest version!	Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OGa48039	XM_015280248.1	Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTCH1 ( NM_204960.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990291.2 Gallus gallus patched 1 (PTCH1), mRNA.

Price & Availability↑

CloneID	OGa28384
Clone ID Related Accession (Same CDS sequence)	NM_204960.2
Accession Version	NM_204960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550937600000
Organism	Gallus gallus(chicken)
Product	protein patched homolog 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC186361.2. On Mar 20, 2015 this sequence version replaced NM_204960.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: U40074.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTCGG CCGCTGACGC CTTAGAGCCG GAGAGCGGCA GCAGCACCGC GGGCGGCGGC 
AGCCACCCCG TTCGGGCGGC GAGGAGTGCC CGCGGCCGGC GGCGGCGGAG CGGAGGGACC 
CGGCGCGCCG CGGCGCCCGA CCGGGAGTAT CTGCAGCGGC CGAGCTACTG CGATGCGGCG 
TTCGCCTTGG AGCAGATCGC AAAGGGGAGA GCTACCGGGA GGAGAGCGCC GCTGTGGCTG 
CGGGCGAAGT TTCAGAGACT GCTCTTTAAC CTGGGCTGCT ACATTCAGAA AAACTGCGGC 
AAGTTCCTGG TGGTCGGCCT GCTGATATTC GGCGCGTTCG CGGTGGGACT ACGGGCGGCC 
AACCTAGAGA CCAACGTAGA GGAGCTGTGG GTGGAAGTTG GTGGACGAGT GAGTCGGGAG 
TTAAACTACA CACGGCAGAA GATTGGAGAA GAGGCCATGT TTAACCCCCA GCTCATGATT 
CAGACTCCAC AGGAAGATGG AACTAATGTA CTCACAACAG AAGCACTCCG GCAGCACCTT 
GACTCTGCAC TTCAGGCCAG CAGAGTACAT GTTTATATGT ACAACAGACA ATGGAAGTTG 
GAACATTTAT GTTATAAATC AGGAGAACTC ATCACAGAAG CAGGCTATAT GGACCAGATT 
ATAGAATATC TTTATCCTTG CTTAATTATC ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA 
AAGCTACAGT CTGGAACTGC CTATCTATTA GGGAAGCCTC CTTTGCAGTG GATCAACTTT 
GACCCCTTAG AATTCTTGGA AGAGTTAAAG AAAATAAACT ACCAAGTGGA GAGCTGGGAA 
GAAATGCTGA ATAAAGCAGA GGTTGGTCAT GGTTATATGG ATCGACCTTG CCTGAATCCT 
GCTGATCCTG ATTGCCCAAT CACAGCTCCC AATAAAAATT CTACCAAGCC TCTTGATGTA 
GCCCTTGTTT TGAGTGGTGG ATGCTATGGA CTGTCAAGAA AATACATGCA TTGGCAGGAA 
GAGTTAATTA TAGGTGGTAC AGTCAAGAAC AGTTCTGGTA AACTTGTCAG TGCCCAGGCT 
TTGCAAACCA TGTTTCAGTT AATGACTCCT AAGCAAATGT ATGAGCACTT CAAGGGATAT 
GAATATGTTT CACATATCAA CTGGAATGAG GATAAAGCAG CAGCAATTCT GGAAGCCTGG 
CAGAGGATGT ATGTCGAGGT TGTTCATCAA AGTGTTGCAC AAAACTCTAC TCAGAAGGTG 
CTTTCCTTTA CTACAACTAC CCTGGACGAC ATCCTAAAGT CATTTTCTGA TGTCAGTGTT 
ATCAGAGTAG CTAGTGGCTA CTTACTAATG CTTGCCTACG CCTGTTTAAC AATGCTGAGG 
TGGGATTGTG CCAAGTCTCA AGGTGCCGTG GGGCTGGCAG GAGTCTTGTT GGTTGCACTG 
TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA CTGATTGGAA TTTCCTTTAA TGCTGCCACA 
ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA GTTGGTGTAG ATGATGTTTT CCTTCTAGCC 
CATGCGTTTA GTGAAACAGG ACAGAATAAG AGAATTCCAT TCGAGGACAG AACAGGTGAA 
TGTTTAAAGC GAACAGGAGC AAGTGTAGCC CTTACATCTA TCAGCAATGT TACTGCATTC 
TTTATGGCTG CCTTAATCCC TATTCCTGCT TTACGAGCAT TTTCACTCCA AGCAGCAGTT 
GTGGTAGTAT TCAACTTTGC AATGGTTCTG CTAATTTTTC CTGCTATACT GAGCATGGAC 
CTTTATCGTC GAGAAGACAG GAGACTGGAT ATATTCTGCT GTTTTACAAG TCCATGTGTC 
ACCAGAGTGA TTCAGATCGA GCCTCAAGCG TATGCAGAAA ATGACAATAT TTGTTACAGT 
TCTCCACCCC CGTACAGCAG TCACAGTTTT GCACATGAAA CTCAGATTAC AATGCAGTCT 
ACAGTGCAGT TGCGCACTGA ATATGATCCT CATACACAAG CGTACTACAC CACTGCAGAG 
CCACGATCAG AAATATCAGT ACAGCCTGTG ACAGTGACTC AAGATAGTCT TAGCTGCCAA 
AGCCCAGAAA GCGCAAGCTC AACAAGGGAT TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG 
CATTGCCTTG AGCCACCCTG TACGAAGTGG ACACTCTCAA CTTTTGCAGA AAAACATTAT 
GCTCCGTTCC TCCTAAAACC AAAAGCTAAG GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA 
CTTGGGCTCA GCCTCTATGG AACTACCCGG GTGAGAGATG GACTAGATCT CACAGACATT 
GTACCACGGG ATACACGGGA ATATGACTTT ATTGCTGCGC AGTTCAAATA CTTCTCATTC 
TACAATATGT ACATTGTGAC ACAGAAAGCA GACTATCCAA ATGTCCAGCA CTTACTTTAC 
GAACTTCACA GAAGTTTCAG CAATGTGACA TATGTTTTGT TGGAAGGAGA TAGGCAGCTT 
CCCAAAATGT GGTTACATTA CTTTCGAGAC TGGCTGCAAG GACTCCAGGA TGCATTTGAC 
AGTGACTGGG AAACTGGGAA AATCACTTAC AGCAACTATA AAAATGGATC TGATGATGCT 
GTTCTGGCTT ATAAACTCTT GGTACAAACA GGCAACCGAG CAAAGCCCAT TGACATCAGT 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGAATCA TTAACCCAAA TGCTTTCTAC 
ATCTACCTGA CAGCCTGGGT TAGCAATGAC CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC 
ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT GACAAAGCAG ACTATATGCC AGAAACAAGG 
CTAAGAATTC CAGCGGCTGA GCCCATAGAA TATGCTCAGT TTCCTTTCTA CCTCAATGGA 
TTACGTGAGA CTTCTGACTT TGTGGAAGCT ATTGAGAAAG TGAGAGCTAT CTGCAATAAC 
TACACAAGCC TCGGGATCGC TAGCTACCCA AATGGTTACC CATTTCTATT CTGGGAGCAG 
TACATTGGGC TTCGTCATTG GCTCCTCTTA TCCATTAGCG TGGTTTTGGC TTGCACGTTT 
CTGGTGTGTG CCCTCTTCCT CCTAAACCCC TGGACAGCTG GGATCATTGT GGTGGTGCTG 
GCTCTGATGA CTGTGGAGCT GTTTGGCATG ATGGGTTTAA TTGGAATAAA ACTAAGTGCA 
GTGCCTGTGG TTATCCTGAT TGCTTCTGTT GGAATTGGTG TGGAATTCAC TGTTCACATT 
GCTTTGGCAT TTTTAACTGC CATAGGAGAT AAGAACCGTA GGGCTGTCCT TGCATTGGAA 
CATATGTTTG CACCAGTGTT GGATGGAGCT GTGTCCACTC TTCTTGGAGT GTTAATGCTT 
GCAGGATCAG AGTTTGATTT TATTGTCAGG TATTTCTTTG CTGTTTTGGC AATCTTAACC 
ATTCTGGGAG TTCTCAATGG ATTGGTGCTG CTTCCTGTTC TTCTTTCTTT CTTTGGACCA 
TACCCTGAGG TTTCTCCAGC CTGTGGTCGA AACAGATTAC CTACACCATC TCCTGAGCCA 
CCTCCGAGTA TTGTGAGGTT TGCACTGCCG CCTGGGCACA CAAATAATGG GTCAGATTCG 
TCTGATTCAG AGTACAGCTC TCAAACCACC GTGTCAGGCA TCAGTGAGGA GCTCCATCAT 
TATGAGGCCA CCCAAAGCCC TGGGATACCA GTCCATCAAG TAGTAGTGGA AGCCACTGAG 
AATCCTGTCT TTGCAAGATC CACTGTGGTA CAGCCAGAGT CAAGGCATCA GTCAAGTCCC 
AGATTGCAAT CAAATCCAGA GGCTGGCACA CAGCAGGTGT GGCATCAGGG CAGACAACCC 
AAGCAGGAAG TGAGGGAAGG ACTGCGACCA CCTCCTTACC GCCCTCGCAG GGACGCTTTT 
GAAATTTCTA CTGAAGGGCA TTCAGGACCT AGCAATAAGG ATCGCTTGAA CCACAAGGCT 
CATTCTCATA ACATGAGAAG CCCAGCATTT GGTGCCATGG GTGTTCCAGG ATCTGCCTAC 
TGCCAGCCTA TCACTACTGT GACTGCTTCG GCTTCAGTGA CTGTTGCTGT CCACCCTGCT 
GTGCACAGCC ACAACTCTTG CAGAGGGAGC TTTCCATCCT GTGAAGAATA CAATGAAGAT 
GACCGTGAGA TGTTTGAGGA CCCCCATGTG CCTTTTAATG TGCGGTGTGA AAGAAGAAAT 
TCTAAAGTAG AAGTTATAGA GCTTCAGGAT GTCGAATGTG AGGAGAGGAC AGCTGGCAAA 
ATCTCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990291.2
CDS	19..4350
Translation	MASAADALEPESGSSTAGGGSHPVRAARSARGRRRRSGGTRRAAAPDREYLQRPSYCDAAFALEQIAKGRATGRRAPLWLRAKFQRLLFNLGCYIQKNCGKFLVVGLLIFGAFAVGLRAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPQEDGTNVLTTEALRQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITEAGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLQWINFDPLEFLEELKKINYQVESWEEMLNKAEVGHGYMDRPCLNPADPDCPITAPNKNSTKPLDVALVLSGGCYGLSRKYMHWQEELIIGGTVKNSSGKLVSAQALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRMYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCAKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVTRVIQIEPQAYAENDNICYSSPPPYSSHSFAHETQITMQSTVQLRTEYDPHTQAYYTTAEPRSEISVQPVTVTQDSLSCQSPESASSTRDLLSQFSDSSVHCLEPPCTKWTLSTFAEKHYAPFLLKPKAKVVVIFLFLGLLGLSLYGTTRVRDGLDLTDIVPRDTREYDFIAAQFKYFSFYNMYIVTQKADYPNVQHLLYELHRSFSNVTYVLLEGDRQLPKMWLHYFRDWLQGLQDAFDSDWETGKITYSNYKNGSDDAVLAYKLLVQTGNRAKPIDISQLTKQRLVDADGIINPNAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRETSDFVEAIEKVRAICNNYTSLGIASYPNGYPFLFWEQYIGLRHWLLLSISVVLACTFLVCALFLLNPWTAGIIVVVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHIALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPACGRNRLPTPSPEPPPSIVRFALPPGHTNNGSDSSDSEYSSQTTVSGISEELHHYEATQSPGIPVHQVVVEATENPVFARSTVVQPESRHQSSPRLQSNPEAGTQQVWHQGRQPKQEVREGLRPPPYRPRRDAFEISTEGHSGPSNKDRLNHKAHSHNMRSPAFGAMGVPGSAYCQPITTVTASASVTVAVHPAVHSHNSCRGSFPSCEEYNEDDREMFEDPHVPFNVRCERRNSKVEVIELQDVECEERTAGKISE

Target ORF information:

RefSeq Version	NM_204960.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus patched 1 (PTCH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204960.2

ORF Insert Sequence:

ATGGCCTCGG CCGCTGACGC CTTAGAGCCG GAGAGCGGCA GCAGCACCGC GGGCGGCGGC 
AGCCACCCCG TTCGGGCGGC GAGGAGTGCC CGCGGCCGGC GGCGGCGGAG CGGAGGGACC 
CGGCGCGCCG CGGCGCCCGA CCGGGAGTAT CTGCAGCGGC CGAGCTACTG CGATGCGGCG 
TTCGCCTTGG AGCAGATCGC AAAGGGGAGA GCTACCGGGA GGAGAGCGCC GCTGTGGCTG 
CGGGCGAAGT TTCAGAGACT GCTCTTTAAC CTGGGCTGCT ACATTCAGAA AAACTGCGGC 
AAGTTCCTGG TGGTCGGCCT GCTGATATTC GGCGCGTTCG CGGTGGGACT ACGGGCGGCC 
AACCTAGAGA CCAACGTAGA GGAGCTGTGG GTGGAAGTTG GTGGACGAGT GAGTCGGGAG 
TTAAACTACA CACGGCAGAA GATTGGAGAA GAGGCCATGT TTAACCCCCA GCTCATGATT 
CAGACTCCAC AGGAAGATGG AACTAATGTA CTCACAACAG AAGCACTCCG GCAGCACCTT 
GACTCTGCAC TTCAGGCCAG CAGAGTACAT GTTTATATGT ACAACAGACA ATGGAAGTTG 
GAACATTTAT GTTATAAATC AGGAGAACTC ATCACAGAAG CAGGCTATAT GGACCAGATT 
ATAGAATATC TTTATCCTTG CTTAATTATC ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA 
AAGCTACAGT CTGGAACTGC CTATCTATTA GGGAAGCCTC CTTTGCAGTG GATCAACTTT 
GACCCCTTAG AATTCTTGGA AGAGTTAAAG AAAATAAACT ACCAAGTGGA GAGCTGGGAA 
GAAATGCTGA ATAAAGCAGA GGTTGGTCAT GGTTATATGG ATCGACCTTG CCTGAATCCT 
GCTGATCCTG ATTGCCCAAT CACAGCTCCC AATAAAAATT CTACCAAGCC TCTTGATGTA 
GCCCTTGTTT TGAGTGGTGG ATGCTATGGA CTGTCAAGAA AATACATGCA TTGGCAGGAA 
GAGTTAATTA TAGGTGGTAC AGTCAAGAAC AGTTCTGGTA AACTTGTCAG TGCCCAGGCT 
TTGCAAACCA TGTTTCAGTT AATGACTCCT AAGCAAATGT ATGAGCACTT CAAGGGATAT 
GAATATGTTT CACATATCAA CTGGAATGAG GATAAAGCAG CAGCAATTCT GGAAGCCTGG 
CAGAGGATGT ATGTCGAGGT TGTTCATCAA AGTGTTGCAC AAAACTCTAC TCAGAAGGTG 
CTTTCCTTTA CTACAACTAC CCTGGACGAC ATCCTAAAGT CATTTTCTGA TGTCAGTGTT 
ATCAGAGTAG CTAGTGGCTA CTTACTAATG CTTGCCTACG CCTGTTTAAC AATGCTGAGG 
TGGGATTGTG CCAAGTCTCA AGGTGCCGTG GGGCTGGCAG GAGTCTTGTT GGTTGCACTG 
TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA CTGATTGGAA TTTCCTTTAA TGCTGCCACA 
ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA GTTGGTGTAG ATGATGTTTT CCTTCTAGCC 
CATGCGTTTA GTGAAACAGG ACAGAATAAG AGAATTCCAT TCGAGGACAG AACAGGTGAA 
TGTTTAAAGC GAACAGGAGC AAGTGTAGCC CTTACATCTA TCAGCAATGT TACTGCATTC 
TTTATGGCTG CCTTAATCCC TATTCCTGCT TTACGAGCAT TTTCACTCCA AGCAGCAGTT 
GTGGTAGTAT TCAACTTTGC AATGGTTCTG CTAATTTTTC CTGCTATACT GAGCATGGAC 
CTTTATCGTC GAGAAGACAG GAGACTGGAT ATATTCTGCT GTTTTACAAG TCCATGTGTC 
ACCAGAGTGA TTCAGATCGA GCCTCAAGCG TATGCAGAAA ATGACAATAT TTGTTACAGT 
TCTCCACCCC CGTACAGCAG TCACAGTTTT GCACATGAAA CTCAGATTAC AATGCAGTCT 
ACAGTGCAGT TGCGCACTGA ATATGATCCT CATACACAAG CGTACTACAC CACTGCAGAG 
CCACGATCAG AAATATCAGT ACAGCCTGTG ACAGTGACTC AAGATAGTCT TAGCTGCCAA 
AGCCCAGAAA GCGCAAGCTC AACAAGGGAT TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG 
CATTGCCTTG AGCCACCCTG TACGAAGTGG ACACTCTCAA CTTTTGCAGA AAAACATTAT 
GCTCCGTTCC TCCTAAAACC AAAAGCTAAG GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA 
CTTGGGCTCA GCCTCTATGG AACTACCCGG GTGAGAGATG GACTAGATCT CACAGACATT 
GTACCACGGG ATACACGGGA ATATGACTTT ATTGCTGCGC AGTTCAAATA CTTCTCATTC 
TACAATATGT ACATTGTGAC ACAGAAAGCA GACTATCCAA ATGTCCAGCA CTTACTTTAC 
GAACTTCACA GAAGTTTCAG CAATGTGACA TATGTTTTGT TGGAAGGAGA TAGGCAGCTT 
CCCAAAATGT GGTTACATTA CTTTCGAGAC TGGCTGCAAG GACTCCAGGA TGCATTTGAC 
AGTGACTGGG AAACTGGGAA AATCACTTAC AGCAACTATA AAAATGGATC TGATGATGCT 
GTTCTGGCTT ATAAACTCTT GGTACAAACA GGCAACCGAG CAAAGCCCAT TGACATCAGT 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGAATCA TTAACCCAAA TGCTTTCTAC 
ATCTACCTGA CAGCCTGGGT TAGCAATGAC CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC 
ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT GACAAAGCAG ACTATATGCC AGAAACAAGG 
CTAAGAATTC CAGCGGCTGA GCCCATAGAA TATGCTCAGT TTCCTTTCTA CCTCAATGGA 
TTACGTGAGA CTTCTGACTT TGTGGAAGCT ATTGAGAAAG TGAGAGCTAT CTGCAATAAC 
TACACAAGCC TCGGGATCGC TAGCTACCCA AATGGTTACC CATTTCTATT CTGGGAGCAG 
TACATTGGGC TTCGTCATTG GCTCCTCTTA TCCATTAGCG TGGTTTTGGC TTGCACGTTT 
CTGGTGTGTG CCCTCTTCCT CCTAAACCCC TGGACAGCTG GGATCATTGT GGTGGTGCTG 
GCTCTGATGA CTGTGGAGCT GTTTGGCATG ATGGGTTTAA TTGGAATAAA ACTAAGTGCA 
GTGCCTGTGG TTATCCTGAT TGCTTCTGTT GGAATTGGTG TGGAATTCAC TGTTCACATT 
GCTTTGGCAT TTTTAACTGC CATAGGAGAT AAGAACCGTA GGGCTGTCCT TGCATTGGAA 
CATATGTTTG CACCAGTGTT GGATGGAGCT GTGTCCACTC TTCTTGGAGT GTTAATGCTT 
GCAGGATCAG AGTTTGATTT TATTGTCAGG TATTTCTTTG CTGTTTTGGC AATCTTAACC 
ATTCTGGGAG TTCTCAATGG ATTGGTGCTG CTTCCTGTTC TTCTTTCTTT CTTTGGACCA 
TACCCTGAGG TTTCTCCAGC CTGTGGTCGA AACAGATTAC CTACACCATC TCCTGAGCCA 
CCTCCGAGTA TTGTGAGGTT TGCACTGCCG CCTGGGCACA CAAATAATGG GTCAGATTCG 
TCTGATTCAG AGTACAGCTC TCAAACCACC GTGTCAGGCA TCAGTGAGGA GCTCCATCAT 
TATGAGGCCA CCCAAAGCCC TGGGATACCA GTCCATCAAG TAGTAGTGGA AGCCACTGAG 
AATCCTGTCT TTGCAAGATC CACTGTGGTA CAGCCAGAGT CAAGGCATCA GTCAAGTCCC 
AGATTGCAAT CAAATCCAGA GGCTGGCACA CAGCAGGTGT GGCATCAGGG CAGACAACCC 
AAGCAGGAAG TGAGGGAAGG ACTGCGACCA CCTCCTTACC GCCCTCGCAG GGACGCTTTT 
GAAATTTCTA CTGAAGGGCA TTCAGGACCT AGCAATAAGG ATCGCTTGAA CCACAAGGCT 
CATTCTCATA ACATGAGAAG CCCAGCATTT GGTGCCATGG GTGTTCCAGG ATCTGCCTAC 
TGCCAGCCTA TCACTACTGT GACTGCTTCG GCTTCAGTGA CTGTTGCTGT CCACCCTGCT 
GTGCACAGCC ACAACTCTTG CAGAGGGAGC TTTCCATCCT GTGAAGAATA CAATGAAGAT 
GACCGTGAGA TGTTTGAGGA CCCCCATGTG CCTTTTAATG TGCGGTGTGA AAGAAGAAAT 
TCTAAAGTAG AAGTTATAGA GCTTCAGGAT GTCGAATGTG AGGAGAGGAC AGCTGGCAAA 
ATCTCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_025144670.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025000438.1 Gallus gallus patched 1 (PTCH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa152959
Clone ID Related Accession (Same CDS sequence)	XM_025144670.1
Accession Version	XM_025144670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	protein patched homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACC CCCAGCTCAT GATTCAGACT CCACAGGAAG ATGGAACTAA TGTACTCACA 
ACAGAAGCAC TCCGGCAGCA CCTTGACTCT GCACTTCAGG CCAGCAGAGT ACATGTTTAT 
ATGTACAACA GACAATGGAA GTTGGAACAT TTATGTTATA AATCAGGAGA ACTCATCACA 
GAAGCAGGCT ATATGGACCA GATTATAGAA TATCTTTATC CTTGCTTAAT TATCACTCCT 
TTGGACTGCT TCTGGGAGGG AGCAAAGCTA CAGTCTGGAA CTGCCTATCT ATTAGGGAAG 
CCTCCTTTGC AGTGGATCAA CTTTGACCCC TTAGAATTCT TGGAAGAGTT AAAGAAAATA 
AACTACCAAG TGGAGAGCTG GGAAGAAATG CTGAATAAAG CAGAGGTTGG TCATGGTTAT 
ATGGATCGAC CTTGCCTGAA TCCTGCTGAT CCTGATTGCC CAATCACAGC TCCCAATAAA 
AATTCTACCA AGCCTCTTGA TGTAGCCCTT GTTTTGAGTG GTGGATGCTA TGGACTGTCA 
AGAAAATACA TGCATTGGCA GGAAGAGTTA ATTATAGGTG GTACAGTCAA GAACAGTTCT 
GGTAAACTTG TCAGTGCCCA GGCTTTGCAA ACCATGTTTC AGTTAATGAC TCCTAAGCAA 
ATGTATGAGC ACTTCAAGGG ATATGAATAT GTTTCACATA TCAACTGGAA TGAGGATAAA 
GCAGCAGCAA TTCTGGAAGC CTGGCAGAGG ATGTATGTCG AGGTTGTTCA TCAAAGTGTT 
GCACAAAACT CTACTCAGAA GGTGCTTTCC TTTACTACAA CTACCCTGGA CGACATCCTA 
AAGTCATTTT CTGATGTCAG TGTTATCAGA GTAGCTAGTG GCTACTTACT AATGCTTGCC 
TACGCCTGTT TAACAATGCT GAGGTGGGAT TGTGCCAAGT CTCAAGGTGC CGTGGGGCTG 
GCAGGAGTCT TGTTGGTTGC ACTGTCAGTG GCTGCAGGCT TGGGTCTGTG CTCACTGATT 
GGAATTTCCT TTAATGCTGC CACAACTCAG GTTCTGCCTT TTCTTGCTCT CGGAGTTGGT 
GTAGATGATG TTTTCCTTCT AGCCCATGCG TTTAGTGAAA CAGGACAGAA TAAGAGAATT 
CCATTCGAGG ACAGAACAGG TGAATGTTTA AAGCGAACAG GAGCAAGTGT AGCCCTTACA 
TCTATCAGCA ATGTTACTGC ATTCTTTATG GCTGCCTTAA TCCCTATTCC TGCTTTACGA 
GCATTTTCAC TCCAAGCAGC AGTTGTGGTA GTATTCAACT TTGCAATGGT TCTGCTAATT 
TTTCCTGCTA TACTGAGCAT GGACCTTTAT CGTCGAGAAG ACAGGAGACT GGATATATTC 
TGCTGTTTTA CAAGTCCATG TGTCACCAGA GTGATTCAGA TCGAGCCTCA AGCGTATGCA 
GAAAATGACA ATATTTGTTA CAGTTCTCCA CCCCCGTACA GCAGTCACAG TTTTGCACAT 
GAAACTCAGA TTACAATGCA GTCTACAGTG CAGTTGCGCA CTGAATATGA TCCTCATACA 
CAAGCGTACT ACACCACTGC AGAGCCACGA TCAGAAATAT CAGTACAGCC TGTGACAGTG 
ACTCAAGATA GTCTTAGCTG CCAAAGCCCA GAAAGCGCAA GCTCAACAAG GGATTTGCTT 
TCCCAGTTCT CAGACTCCAG TGTGCATTGC CTTGAGCCAC CCTGTACGAA GTGGACACTC 
TCAACTTTTG CAGAAAAACA TTATGCTCCG TTCCTCCTAA AACCAAAAGC TAAGGTTGTG 
GTTATTTTTC TTTTCCTGGG CTTACTTGGG CTCAGCCTCT ATGGAACTAC CCGGGTGAGA 
GATGGACTAG ATCTCACAGA CATTGTACCA CGGGATACAC GGGAATATGA CTTTATTGCT 
GCGCAGTTCA AATACTTCTC ATTCTACAAT ATGTACATTG TGACACAGAA AGCAGACTAT 
CCAAATGTCC AGCACTTACT TTACGAACTT CACAGAAGTT TCAGCAATGT GACATATGTT 
TTGTTGGAAG GAGATAGGCA GCTTCCCAAA ATGTGGTTAC ATTACTTTCG AGACTGGCTG 
CAAGGACTCC AGGATGCATT TGACAGTGAC TGGGAAACTG GGAAAATCAC TTACAGCAAC 
TATAAAAATG GATCTGATGA TGCTGTTCTG GCTTATAAAC TCTTGGTACA AACAGGCAAC 
CGAGCAAAGC CCATTGACAT CAGTCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGA 
ATCATTAACC CAAATGCTTT CTACATCTAC CTGACAGCCT GGGTTAGCAA TGACCCTGTG 
GCCTATGCTG CCTCTCAAGC CAACATCCGG CCTCACCGCC CAGAATGGGT GCATGACAAA 
GCAGACTATA TGCCAGAAAC AAGGCTAAGA ATTCCAGCGG CTGAGCCCAT AGAATATGCT 
CAGTTTCCTT TCTACCTCAA TGGATTACGT GAGACTTCTG ACTTTGTGGA AGCTATTGAG 
AAAGTGAGAG CTATCTGCAA TAACTACACA AGCCTCGGGA TCGCTAGCTA CCCAAATGGT 
TACCCATTTC TATTCTGGGA GCAGTACATT GGGCTTCGTC ATTGGCTCCT CTTATCCATT 
AGCGTGGTTT TGGCTTGCAC GTTTCTGGTG TGTGCCCTCT TCCTCCTAAA CCCCTGGACA 
GCTGGGATCA TTGTGGTGGT GCTGGCTCTG ATGACTGTGG AGCTGTTTGG CATGATGGGT 
TTAATTGGAA TAAAACTAAG TGCAGTGCCT GTGGTTATCC TGATTGCTTC TGTTGGAATT 
GGTGTGGAAT TCACTGTTCA CATTGCTTTG GCATTTTTAA CTGCCATAGG AGATAAGAAC 
CGTAGGGCTG TCCTTGCATT GGAACATATG TTTGCACCAG TGTTGGATGG AGCTGTGTCC 
ACTCTTCTTG GAGTGTTAAT GCTTGCAGGA TCAGAGTTTG ATTTTATTGT CAGGTATTTC 
TTTGCTGTTT TGGCAATCTT AACCATTCTG GGAGTTCTCA ATGGATTGGT GCTGCTTCCT 
GTTCTTCTTT CTTTCTTTGG ACCATACCCT GAGGTTTCTC CAGCCTGTGG TCGAAACAGA 
TTACCTACAC CATCTCCTGA GCCACCTCCG AGTATTGTGA GGTTTGCACT GCCGCCTGGG 
CACACAAATA ATGGGTCAGA TTCGTCTGAT TCAGAGTACA GCTCTCAAAC CACCGTGTCA 
GGCATCAGTG AGGAGCTCCA TCATTATGAG GCCACCCAAA GCCCTGGGAT ACCAGTCCAT 
CAAGTAGTAG TGGAAGCCAC TGAGAATCCT GTCTTTGCAA GATCCACTGT GGTACAGCCA 
GAGTCAAGGC ATCAGTCAAG TCCCAGATTG CAATCAAATC CAGAGGCTGG CACACAGCAG 
GTGTGGCATC AGGGCAGACA ACCCAAGCAG GAAGTGAGGG AAGGACTGCG ACCACCTCCT 
TACCGCCCTC GCAGGGACGC TTTTGAAATT TCTACTGAAG GGCATTCAGG ACCTAGCAAT 
AAGGATCGCT TGAACCACAA GGCTCATTCT CATAACATGA GAAGCCCAGC ATTTGGTGCC 
ATGGGTGTTC CAGGATCTGC CTACTGCCAG CCTATCACTA CTGTGACTGC TTCGGCTTCA 
GTGACTGTTG CTGTCCACCC TGCTGTGCAC AGCCACAACT CTTGCAGAGG GAGCTTTCCA 
TCCTGTGAAG AATACAATGA AGATGACCGT GAGATGTTTG AGGACCCCCA TGTGCCTTTT 
AATGTGCGGT GTGAAAGAAG AAATTCTAAA GTAGAAGTTA TAGAGCTTCA GGATGTCGAA 
TGTGAGGAGA GGACAGCTGG CAAAATCTCA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025000438.1
CDS	122..3997
Translation	MFNPQLMIQTPQEDGTNVLTTEALRQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITEAGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLQWINFDPLEFLEELKKINYQVESWEEMLNKAEVGHGYMDRPCLNPADPDCPITAPNKNSTKPLDVALVLSGGCYGLSRKYMHWQEELIIGGTVKNSSGKLVSAQALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRMYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCAKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVTRVIQIEPQAYAENDNICYSSPPPYSSHSFAHETQITMQSTVQLRTEYDPHTQAYYTTAEPRSEISVQPVTVTQDSLSCQSPESASSTRDLLSQFSDSSVHCLEPPCTKWTLSTFAEKHYAPFLLKPKAKVVVIFLFLGLLGLSLYGTTRVRDGLDLTDIVPRDTREYDFIAAQFKYFSFYNMYIVTQKADYPNVQHLLYELHRSFSNVTYVLLEGDRQLPKMWLHYFRDWLQGLQDAFDSDWETGKITYSNYKNGSDDAVLAYKLLVQTGNRAKPIDISQLTKQRLVDADGIINPNAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRETSDFVEAIEKVRAICNNYTSLGIASYPNGYPFLFWEQYIGLRHWLLLSISVVLACTFLVCALFLLNPWTAGIIVVVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHIALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPACGRNRLPTPSPEPPPSIVRFALPPGHTNNGSDSSDSEYSSQTTVSGISEELHHYEATQSPGIPVHQVVVEATENPVFARSTVVQPESRHQSSPRLQSNPEAGTQQVWHQGRQPKQEVREGLRPPPYRPRRDAFEISTEGHSGPSNKDRLNHKAHSHNMRSPAFGAMGVPGSAYCQPITTVTASASVTVAVHPAVHSHNSCRGSFPSCEEYNEDDREMFEDPHVPFNVRCERRNSKVEVIELQDVECEERTAGKISE

Target ORF information:

RefSeq Version	XM_025144670.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus patched 1 (PTCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025144670.1

ORF Insert Sequence:

ATGTTTAACC CCCAGCTCAT GATTCAGACT CCACAGGAAG ATGGAACTAA TGTACTCACA 
ACAGAAGCAC TCCGGCAGCA CCTTGACTCT GCACTTCAGG CCAGCAGAGT ACATGTTTAT 
ATGTACAACA GACAATGGAA GTTGGAACAT TTATGTTATA AATCAGGAGA ACTCATCACA 
GAAGCAGGCT ATATGGACCA GATTATAGAA TATCTTTATC CTTGCTTAAT TATCACTCCT 
TTGGACTGCT TCTGGGAGGG AGCAAAGCTA CAGTCTGGAA CTGCCTATCT ATTAGGGAAG 
CCTCCTTTGC AGTGGATCAA CTTTGACCCC TTAGAATTCT TGGAAGAGTT AAAGAAAATA 
AACTACCAAG TGGAGAGCTG GGAAGAAATG CTGAATAAAG CAGAGGTTGG TCATGGTTAT 
ATGGATCGAC CTTGCCTGAA TCCTGCTGAT CCTGATTGCC CAATCACAGC TCCCAATAAA 
AATTCTACCA AGCCTCTTGA TGTAGCCCTT GTTTTGAGTG GTGGATGCTA TGGACTGTCA 
AGAAAATACA TGCATTGGCA GGAAGAGTTA ATTATAGGTG GTACAGTCAA GAACAGTTCT 
GGTAAACTTG TCAGTGCCCA GGCTTTGCAA ACCATGTTTC AGTTAATGAC TCCTAAGCAA 
ATGTATGAGC ACTTCAAGGG ATATGAATAT GTTTCACATA TCAACTGGAA TGAGGATAAA 
GCAGCAGCAA TTCTGGAAGC CTGGCAGAGG ATGTATGTCG AGGTTGTTCA TCAAAGTGTT 
GCACAAAACT CTACTCAGAA GGTGCTTTCC TTTACTACAA CTACCCTGGA CGACATCCTA 
AAGTCATTTT CTGATGTCAG TGTTATCAGA GTAGCTAGTG GCTACTTACT AATGCTTGCC 
TACGCCTGTT TAACAATGCT GAGGTGGGAT TGTGCCAAGT CTCAAGGTGC CGTGGGGCTG 
GCAGGAGTCT TGTTGGTTGC ACTGTCAGTG GCTGCAGGCT TGGGTCTGTG CTCACTGATT 
GGAATTTCCT TTAATGCTGC CACAACTCAG GTTCTGCCTT TTCTTGCTCT CGGAGTTGGT 
GTAGATGATG TTTTCCTTCT AGCCCATGCG TTTAGTGAAA CAGGACAGAA TAAGAGAATT 
CCATTCGAGG ACAGAACAGG TGAATGTTTA AAGCGAACAG GAGCAAGTGT AGCCCTTACA 
TCTATCAGCA ATGTTACTGC ATTCTTTATG GCTGCCTTAA TCCCTATTCC TGCTTTACGA 
GCATTTTCAC TCCAAGCAGC AGTTGTGGTA GTATTCAACT TTGCAATGGT TCTGCTAATT 
TTTCCTGCTA TACTGAGCAT GGACCTTTAT CGTCGAGAAG ACAGGAGACT GGATATATTC 
TGCTGTTTTA CAAGTCCATG TGTCACCAGA GTGATTCAGA TCGAGCCTCA AGCGTATGCA 
GAAAATGACA ATATTTGTTA CAGTTCTCCA CCCCCGTACA GCAGTCACAG TTTTGCACAT 
GAAACTCAGA TTACAATGCA GTCTACAGTG CAGTTGCGCA CTGAATATGA TCCTCATACA 
CAAGCGTACT ACACCACTGC AGAGCCACGA TCAGAAATAT CAGTACAGCC TGTGACAGTG 
ACTCAAGATA GTCTTAGCTG CCAAAGCCCA GAAAGCGCAA GCTCAACAAG GGATTTGCTT 
TCCCAGTTCT CAGACTCCAG TGTGCATTGC CTTGAGCCAC CCTGTACGAA GTGGACACTC 
TCAACTTTTG CAGAAAAACA TTATGCTCCG TTCCTCCTAA AACCAAAAGC TAAGGTTGTG 
GTTATTTTTC TTTTCCTGGG CTTACTTGGG CTCAGCCTCT ATGGAACTAC CCGGGTGAGA 
GATGGACTAG ATCTCACAGA CATTGTACCA CGGGATACAC GGGAATATGA CTTTATTGCT 
GCGCAGTTCA AATACTTCTC ATTCTACAAT ATGTACATTG TGACACAGAA AGCAGACTAT 
CCAAATGTCC AGCACTTACT TTACGAACTT CACAGAAGTT TCAGCAATGT GACATATGTT 
TTGTTGGAAG GAGATAGGCA GCTTCCCAAA ATGTGGTTAC ATTACTTTCG AGACTGGCTG 
CAAGGACTCC AGGATGCATT TGACAGTGAC TGGGAAACTG GGAAAATCAC TTACAGCAAC 
TATAAAAATG GATCTGATGA TGCTGTTCTG GCTTATAAAC TCTTGGTACA AACAGGCAAC 
CGAGCAAAGC CCATTGACAT CAGTCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGA 
ATCATTAACC CAAATGCTTT CTACATCTAC CTGACAGCCT GGGTTAGCAA TGACCCTGTG 
GCCTATGCTG CCTCTCAAGC CAACATCCGG CCTCACCGCC CAGAATGGGT GCATGACAAA 
GCAGACTATA TGCCAGAAAC AAGGCTAAGA ATTCCAGCGG CTGAGCCCAT AGAATATGCT 
CAGTTTCCTT TCTACCTCAA TGGATTACGT GAGACTTCTG ACTTTGTGGA AGCTATTGAG 
AAAGTGAGAG CTATCTGCAA TAACTACACA AGCCTCGGGA TCGCTAGCTA CCCAAATGGT 
TACCCATTTC TATTCTGGGA GCAGTACATT GGGCTTCGTC ATTGGCTCCT CTTATCCATT 
AGCGTGGTTT TGGCTTGCAC GTTTCTGGTG TGTGCCCTCT TCCTCCTAAA CCCCTGGACA 
GCTGGGATCA TTGTGGTGGT GCTGGCTCTG ATGACTGTGG AGCTGTTTGG CATGATGGGT 
TTAATTGGAA TAAAACTAAG TGCAGTGCCT GTGGTTATCC TGATTGCTTC TGTTGGAATT 
GGTGTGGAAT TCACTGTTCA CATTGCTTTG GCATTTTTAA CTGCCATAGG AGATAAGAAC 
CGTAGGGCTG TCCTTGCATT GGAACATATG TTTGCACCAG TGTTGGATGG AGCTGTGTCC 
ACTCTTCTTG GAGTGTTAAT GCTTGCAGGA TCAGAGTTTG ATTTTATTGT CAGGTATTTC 
TTTGCTGTTT TGGCAATCTT AACCATTCTG GGAGTTCTCA ATGGATTGGT GCTGCTTCCT 
GTTCTTCTTT CTTTCTTTGG ACCATACCCT GAGGTTTCTC CAGCCTGTGG TCGAAACAGA 
TTACCTACAC CATCTCCTGA GCCACCTCCG AGTATTGTGA GGTTTGCACT GCCGCCTGGG 
CACACAAATA ATGGGTCAGA TTCGTCTGAT TCAGAGTACA GCTCTCAAAC CACCGTGTCA 
GGCATCAGTG AGGAGCTCCA TCATTATGAG GCCACCCAAA GCCCTGGGAT ACCAGTCCAT 
CAAGTAGTAG TGGAAGCCAC TGAGAATCCT GTCTTTGCAA GATCCACTGT GGTACAGCCA 
GAGTCAAGGC ATCAGTCAAG TCCCAGATTG CAATCAAATC CAGAGGCTGG CACACAGCAG 
GTGTGGCATC AGGGCAGACA ACCCAAGCAG GAAGTGAGGG AAGGACTGCG ACCACCTCCT 
TACCGCCCTC GCAGGGACGC TTTTGAAATT TCTACTGAAG GGCATTCAGG ACCTAGCAAT 
AAGGATCGCT TGAACCACAA GGCTCATTCT CATAACATGA GAAGCCCAGC ATTTGGTGCC 
ATGGGTGTTC CAGGATCTGC CTACTGCCAG CCTATCACTA CTGTGACTGC TTCGGCTTCA 
GTGACTGTTG CTGTCCACCC TGCTGTGCAC AGCCACAACT CTTGCAGAGG GAGCTTTCCA 
TCCTGTGAAG AATACAATGA AGATGACCGT GAGATGTTTG AGGACCCCCA TGTGCCTTTT 
AATGTGCGGT GTGAAAGAAG AAATTCTAAA GTAGAAGTTA TAGAGCTTCA GGATGTCGAA 
TGTGAGGAGA GGACAGCTGG CAAAATCTCA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_015280248.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135734.1 Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.

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CloneID	OGa48039
Clone ID Related Accession (Same CDS sequence)	XM_015280248.2 , XM_015280248.1
Accession Version	XM_015280248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	protein patched homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC 
CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC 
GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG 
GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA 
GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA 
CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT 
GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC 
ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC 
ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA 
GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG 
AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT 
GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC 
AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA 
CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC 
AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT 
AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG 
GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA 
AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC 
ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG 
CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG 
GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA 
CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA 
GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG 
AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC 
CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT 
TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG 
CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT 
ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG 
TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT 
GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT 
CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG 
ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT 
TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG 
ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG 
GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG 
GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT 
ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA 
GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA 
TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC 
TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC 
AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA 
GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA 
GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC 
CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT 
GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA 
TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT 
ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA 
AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA 
TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC 
TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG 
ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT 
GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT 
AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT 
GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG 
TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG 
CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA 
AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG 
CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC 
GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA 
GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA 
CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA 
CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA 
CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT 
AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT 
GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG 
GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC 
TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG 
CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT 
GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135734.1
CDS	138..4319
Translation	MRSHPAPSRGSPPAPAAMGRATGRRAPLWLRAKFQRLLFNLGCYIQKNCGKFLVVGLLIFGAFAVGLRAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPQEDGTNVLTTEALRQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITEAGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLQWINFDPLEFLEELKKINYQVESWEEMLNKAEVGHGYMDRPCLNPADPDCPITAPNKNSTKPLDVALVLSGGCYGLSRKYMHWQEELIIGGTVKNSSGKLVSAQALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRMYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCAKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVTRVIQIEPQAYAENDNICYSSPPPYSSHSFAHETQITMQSTVQLRTEYDPHTQAYYTTAEPRSEISVQPVTVTQDSLSCQSPESASSTRDLLSQFSDSSVHCLEPPCTKWTLSTFAEKHYAPFLLKPKAKVVVIFLFLGLLGLSLYGTTRVRDGLDLTDIVPRDTREYDFIAAQFKYFSFYNMYIVTQKADYPNVQHLLYELHRSFSNVTYVLLEGDRQLPKMWLHYFRDWLQGLQDAFDSDWETGKITYSNYKNGSDDAVLAYKLLVQTGNRAKPIDISQLTKQRLVDADGIINPNAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRETSDFVEAIEKVRAICNNYTSLGIASYPNGYPFLFWEQYIGLRHWLLLSISVVLACTFLVCALFLLNPWTAGIIVVVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHIALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPACGRNRLPTPSPEPPPSIVRFALPPGHTNNGSDSSDSEYSSQTTVSGISEELHHYEATQSPGIPVHQVVVEATENPVFARSTVVQPESRHQSSPRLQSNPEAGTQQVWHQGRQPKQEVREGLRPPPYRPRRDAFEISTEGHSGPSNKDRLNHKAHSHNMRSPAFGAMGVPGSAYCQPITTVTASASVTVAVHPAVHSHNSCRGSFPSCEEYNEDDREMFEDPHVPFNVRCERRNSKVEVIELQDVECEERTAGKISE

Target ORF information:

RefSeq Version	XM_015280248.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280248.2

ORF Insert Sequence:

ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC 
CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC 
GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG 
GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA 
GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA 
CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT 
GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC 
ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC 
ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA 
GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG 
AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT 
GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC 
AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA 
CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC 
AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT 
AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG 
GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA 
AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC 
ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG 
CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG 
GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA 
CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA 
GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG 
AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC 
CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT 
TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG 
CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT 
ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG 
TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT 
GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT 
CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG 
ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT 
TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG 
ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG 
GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG 
GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT 
ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA 
GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA 
TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC 
TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC 
AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA 
GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA 
GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC 
CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT 
GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA 
TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT 
ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA 
AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA 
TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC 
TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG 
ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT 
GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT 
AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT 
GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG 
TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG 
CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA 
AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG 
CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC 
GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA 
GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA 
CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA 
CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA 
CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT 
AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT 
GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG 
GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC 
TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG 
CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT 
GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_015280248.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135734.1 Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.

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CloneID	OGa48039
Clone ID Related Accession (Same CDS sequence)	XM_015280248.2 , XM_015280248.1
Accession Version	XM_015280248.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	protein patched homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC 
CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC 
GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG 
GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA 
GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA 
CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT 
GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC 
ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC 
ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA 
GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG 
AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT 
GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC 
AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA 
CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC 
AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT 
AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG 
GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA 
AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC 
ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG 
CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG 
GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA 
CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA 
GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG 
AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC 
CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT 
TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG 
CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT 
ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG 
TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT 
GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT 
CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG 
ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT 
TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG 
ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG 
GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG 
GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT 
ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA 
GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA 
TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC 
TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC 
AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA 
GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA 
GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC 
CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT 
GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA 
TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT 
ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA 
AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA 
TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC 
TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG 
ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT 
GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT 
AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT 
GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG 
TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG 
CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA 
AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG 
CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC 
GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA 
GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA 
CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA 
CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA 
CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT 
AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT 
GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG 
GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC 
TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG 
CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT 
GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135734.1
CDS	113..4294
Translation	MRSHPAPSRGSPPAPAAMGRATGRRAPLWLRAKFQRLLFNLGCYIQKNCGKFLVVGLLIFGAFAVGLRAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPQEDGTNVLTTEALRQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITEAGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLQWINFDPLEFLEELKKINYQVESWEEMLNKAEVGHGYMDRPCLNPADPDCPITAPNKNSTKPLDVALVLSGGCYGLSRKYMHWQEELIIGGTVKNSSGKLVSAQALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRMYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCAKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVTRVIQIEPQAYAENDNICYSSPPPYSSHSFAHETQITMQSTVQLRTEYDPHTQAYYTTAEPRSEISVQPVTVTQDSLSCQSPESASSTRDLLSQFSDSSVHCLEPPCTKWTLSTFAEKHYAPFLLKPKAKVVVIFLFLGLLGLSLYGTTRVRDGLDLTDIVPRDTREYDFIAAQFKYFSFYNMYIVTQKADYPNVQHLLYELHRSFSNVTYVLLEGDRQLPKMWLHYFRDWLQGLQDAFDSDWETGKITYSNYKNGSDDAVLAYKLLVQTGNRAKPIDISQLTKQRLVDADGIINPNAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRETSDFVEAIEKVRAICNNYTSLGIASYPNGYPFLFWEQYIGLRHWLLLSISVVLACTFLVCALFLLNPWTAGIIVVVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHIALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPACGRNRLPTPSPEPPPSIVRFALPPGHTNNGSDSSDSEYSSQTTVSGISEELHHYEATQSPGIPVHQVVVEATENPVFARSTVVQPESRHQSSPRLQSNPEAGTQQVWHQGRQPKQEVREGLRPPPYRPRRDAFEISTEGHSGPSNKDRLNHKAHSHNMRSPAFGAMGVPGSAYCQPITTVTASASVTVAVHPAVHSHNSCRGSFPSCEEYNEDDREMFEDPHVPFNVRCERRNSKVEVIELQDVECEERTAGKISE

Target ORF information:

RefSeq Version	XM_015280248.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus patched 1 (PTCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280248.1

ORF Insert Sequence:

ATGAGGTCGC ACCCGGCCCC GTCAAGAGGC TCCCCGCCGG CTCCGGCAGC CATGGGGAGA 
GCTACCGGGA GGAGAGCGCC GCTGTGGCTG CGGGCGAAGT TTCAGAGACT GCTCTTTAAC 
CTGGGCTGCT ACATTCAGAA AAACTGCGGC AAGTTCCTGG TGGTCGGCCT GCTGATATTC 
GGCGCGTTCG CGGTGGGACT ACGGGCGGCC AACCTAGAGA CCAACGTAGA GGAGCTGTGG 
GTGGAAGTTG GTGGACGAGT GAGTCGGGAG TTAAACTACA CACGGCAGAA GATTGGAGAA 
GAGGCCATGT TTAACCCCCA GCTCATGATT CAGACTCCAC AGGAAGATGG AACTAATGTA 
CTCACAACAG AAGCACTCCG GCAGCACCTT GACTCTGCAC TTCAGGCCAG CAGAGTACAT 
GTTTATATGT ACAACAGACA ATGGAAGTTG GAACATTTAT GTTATAAATC AGGAGAACTC 
ATCACAGAAG CAGGCTATAT GGACCAGATT ATAGAATATC TTTATCCTTG CTTAATTATC 
ACTCCTTTGG ACTGCTTCTG GGAGGGAGCA AAGCTACAGT CTGGAACTGC CTATCTATTA 
GGGAAGCCTC CTTTGCAGTG GATCAACTTT GACCCCTTAG AATTCTTGGA AGAGTTAAAG 
AAAATAAACT ACCAAGTGGA GAGCTGGGAA GAAATGCTGA ATAAAGCAGA GGTTGGTCAT 
GGTTATATGG ATCGACCTTG CCTGAATCCT GCTGATCCTG ATTGCCCAAT CACAGCTCCC 
AATAAAAATT CTACCAAGCC TCTTGATGTA GCCCTTGTTT TGAGTGGTGG ATGCTATGGA 
CTGTCAAGAA AATACATGCA TTGGCAGGAA GAGTTAATTA TAGGTGGTAC AGTCAAGAAC 
AGTTCTGGTA AACTTGTCAG TGCCCAGGCT TTGCAAACCA TGTTTCAGTT AATGACTCCT 
AAGCAAATGT ATGAGCACTT CAAGGGATAT GAATATGTTT CACATATCAA CTGGAATGAG 
GATAAAGCAG CAGCAATTCT GGAAGCCTGG CAGAGGATGT ATGTCGAGGT TGTTCATCAA 
AGTGTTGCAC AAAACTCTAC TCAGAAGGTG CTTTCCTTTA CTACAACTAC CCTGGACGAC 
ATCCTAAAGT CATTTTCTGA TGTCAGTGTT ATCAGAGTAG CTAGTGGCTA CTTACTAATG 
CTTGCCTACG CCTGTTTAAC AATGCTGAGG TGGGATTGTG CCAAGTCTCA AGGTGCCGTG 
GGGCTGGCAG GAGTCTTGTT GGTTGCACTG TCAGTGGCTG CAGGCTTGGG TCTGTGCTCA 
CTGATTGGAA TTTCCTTTAA TGCTGCCACA ACTCAGGTTC TGCCTTTTCT TGCTCTCGGA 
GTTGGTGTAG ATGATGTTTT CCTTCTAGCC CATGCGTTTA GTGAAACAGG ACAGAATAAG 
AGAATTCCAT TCGAGGACAG AACAGGTGAA TGTTTAAAGC GAACAGGAGC AAGTGTAGCC 
CTTACATCTA TCAGCAATGT TACTGCATTC TTTATGGCTG CCTTAATCCC TATTCCTGCT 
TTACGAGCAT TTTCACTCCA AGCAGCAGTT GTGGTAGTAT TCAACTTTGC AATGGTTCTG 
CTAATTTTTC CTGCTATACT GAGCATGGAC CTTTATCGTC GAGAAGACAG GAGACTGGAT 
ATATTCTGCT GTTTTACAAG TCCATGTGTC ACCAGAGTGA TTCAGATCGA GCCTCAAGCG 
TATGCAGAAA ATGACAATAT TTGTTACAGT TCTCCACCCC CGTACAGCAG TCACAGTTTT 
GCACATGAAA CTCAGATTAC AATGCAGTCT ACAGTGCAGT TGCGCACTGA ATATGATCCT 
CATACACAAG CGTACTACAC CACTGCAGAG CCACGATCAG AAATATCAGT ACAGCCTGTG 
ACAGTGACTC AAGATAGTCT TAGCTGCCAA AGCCCAGAAA GCGCAAGCTC AACAAGGGAT 
TTGCTTTCCC AGTTCTCAGA CTCCAGTGTG CATTGCCTTG AGCCACCCTG TACGAAGTGG 
ACACTCTCAA CTTTTGCAGA AAAACATTAT GCTCCGTTCC TCCTAAAACC AAAAGCTAAG 
GTTGTGGTTA TTTTTCTTTT CCTGGGCTTA CTTGGGCTCA GCCTCTATGG AACTACCCGG 
GTGAGAGATG GACTAGATCT CACAGACATT GTACCACGGG ATACACGGGA ATATGACTTT 
ATTGCTGCGC AGTTCAAATA CTTCTCATTC TACAATATGT ACATTGTGAC ACAGAAAGCA 
GACTATCCAA ATGTCCAGCA CTTACTTTAC GAACTTCACA GAAGTTTCAG CAATGTGACA 
TATGTTTTGT TGGAAGGAGA TAGGCAGCTT CCCAAAATGT GGTTACATTA CTTTCGAGAC 
TGGCTGCAAG GACTCCAGGA TGCATTTGAC AGTGACTGGG AAACTGGGAA AATCACTTAC 
AGCAACTATA AAAATGGATC TGATGATGCT GTTCTGGCTT ATAAACTCTT GGTACAAACA 
GGCAACCGAG CAAAGCCCAT TGACATCAGT CAGTTGACTA AACAGCGTCT GGTGGATGCA 
GATGGAATCA TTAACCCAAA TGCTTTCTAC ATCTACCTGA CAGCCTGGGT TAGCAATGAC 
CCTGTGGCCT ATGCTGCCTC TCAAGCCAAC ATCCGGCCTC ACCGCCCAGA ATGGGTGCAT 
GACAAAGCAG ACTATATGCC AGAAACAAGG CTAAGAATTC CAGCGGCTGA GCCCATAGAA 
TATGCTCAGT TTCCTTTCTA CCTCAATGGA TTACGTGAGA CTTCTGACTT TGTGGAAGCT 
ATTGAGAAAG TGAGAGCTAT CTGCAATAAC TACACAAGCC TCGGGATCGC TAGCTACCCA 
AATGGTTACC CATTTCTATT CTGGGAGCAG TACATTGGGC TTCGTCATTG GCTCCTCTTA 
TCCATTAGCG TGGTTTTGGC TTGCACGTTT CTGGTGTGTG CCCTCTTCCT CCTAAACCCC 
TGGACAGCTG GGATCATTGT GGTGGTGCTG GCTCTGATGA CTGTGGAGCT GTTTGGCATG 
ATGGGTTTAA TTGGAATAAA ACTAAGTGCA GTGCCTGTGG TTATCCTGAT TGCTTCTGTT 
GGAATTGGTG TGGAATTCAC TGTTCACATT GCTTTGGCAT TTTTAACTGC CATAGGAGAT 
AAGAACCGTA GGGCTGTCCT TGCATTGGAA CATATGTTTG CACCAGTGTT GGATGGAGCT 
GTGTCCACTC TTCTTGGAGT GTTAATGCTT GCAGGATCAG AGTTTGATTT TATTGTCAGG 
TATTTCTTTG CTGTTTTGGC AATCTTAACC ATTCTGGGAG TTCTCAATGG ATTGGTGCTG 
CTTCCTGTTC TTCTTTCTTT CTTTGGACCA TACCCTGAGG TTTCTCCAGC CTGTGGTCGA 
AACAGATTAC CTACACCATC TCCTGAGCCA CCTCCGAGTA TTGTGAGGTT TGCACTGCCG 
CCTGGGCACA CAAATAATGG GTCAGATTCG TCTGATTCAG AGTACAGCTC TCAAACCACC 
GTGTCAGGCA TCAGTGAGGA GCTCCATCAT TATGAGGCCA CCCAAAGCCC TGGGATACCA 
GTCCATCAAG TAGTAGTGGA AGCCACTGAG AATCCTGTCT TTGCAAGATC CACTGTGGTA 
CAGCCAGAGT CAAGGCATCA GTCAAGTCCC AGATTGCAAT CAAATCCAGA GGCTGGCACA 
CAGCAGGTGT GGCATCAGGG CAGACAACCC AAGCAGGAAG TGAGGGAAGG ACTGCGACCA 
CCTCCTTACC GCCCTCGCAG GGACGCTTTT GAAATTTCTA CTGAAGGGCA TTCAGGACCT 
AGCAATAAGG ATCGCTTGAA CCACAAGGCT CATTCTCATA ACATGAGAAG CCCAGCATTT 
GGTGCCATGG GTGTTCCAGG ATCTGCCTAC TGCCAGCCTA TCACTACTGT GACTGCTTCG 
GCTTCAGTGA CTGTTGCTGT CCACCCTGCT GTGCACAGCC ACAACTCTTG CAGAGGGAGC 
TTTCCATCCT GTGAAGAATA CAATGAAGAT GACCGTGAGA TGTTTGAGGA CCCCCATGTG 
CCTTTTAATG TGCGGTGTGA AAGAAGAAAT TCTAAAGTAG AAGTTATAGA GCTTCAGGAT 
GTCGAATGTG AGGAGAGGAC AGCTGGCAAA ATCTCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
Nature431(7011)946-57(2004 Oct)
Jaillon O,Aury JM,Brunet F,Petit JL,Stange-Thomann N,Mauceli E,Bouneau L,Fischer C,Ozouf-Costaz C,Bernot A,Nicaud S,Jaffe D,Fisher S,Lutfalla G,Dossat C,Segurens B,Dasilva C,Salanoubat M,Levy M,Boudet N,Castellano S,Anthouard V,Jubin C,Castelli V,Katinka M,Vacherie B,Biémont C,Skalli Z,Cattolico L,Poulain J,De Berardinis V,Cruaud C,Duprat S,Brottier P,Coutanceau JP,Gouzy J,Parra G,Lardier G,Chapple C,McKernan KJ,McEwan P,Bosak S,Kellis M,Volff JN,Guigó R,Zody MC,Mesirov J,Lindblad-Toh K,Birren B,Nusbaum C,Kahn D,Robinson-Rechavi M,Laudet V,Schachter V,Quétier F,Saurin W,Scarpelli C,Wincker P,Lander ES,Weissenbach J,Roest Crollius H

Male-biased mutation rate and divergence in autosomal, z-linked and w-linked introns of chicken and Turkey.
Molecular biology and evolution21(8)1538-47(2004 Aug)
Axelsson E,Smith NG,Sundström H,Berlin S,Ellegren H

Biochemical evidence that patched is the Hedgehog receptor.
Nature384(6605)176-9(1996 Nov)
Marigo V,Davey RA,Zuo Y,Cunningham JM,Tabin CJ

Conservation in hedgehog signaling: induction of a chicken patched homolog by Sonic hedgehog in the developing limb.
Development (Cambridge, England)122(4)1225-33(1996 Apr)
Marigo V,Scott MP,Johnson RL,Goodrich LV,Tabin CJ

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PTCH1 cDNA ORF clone, Gallus gallus(chicken)

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