Ptch1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptch1
Entrez Gene ID	19206
Full Name	patched 1
Synonyms	A230106A15Rik,Ptc,Ptc1,Ptch,mes,wig
General protein information	Preferred Names patched 1 Names protein patched homolog 1 Ptch1 protein +12b mesenchymal dysplasia patched homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 B3\|13 32.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006517163.3	XP_006517226.1	protein patched homolog 1 isoform X3
NM_008957.3	NP_032983.1	protein patched homolog 1 isoform a
XM_011244497.2	XP_011242799.1	protein patched homolog 1 isoform X4
XM_006517160.3	XP_006517223.1	protein patched homolog 1 isoform X2
XM_006517162.3	XP_006517225.1	protein patched homolog 1 isoform X3
NM_001328514.1	NP_001315443.1	protein patched homolog 1 isoform b
XM_006517159.3	XP_006517222.1	protein patched homolog 1 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PTCH1	XP_001111868.2
Canis lupus familiaris (dog)	PTCH1	XP_003638919.1
Rattus norvegicus (Norway rat)	Ptch1	NP_446018.1
Gallus gallus (chicken)	PTCH1	NP_990291.1
Caenorhabditis elegans (roundworm)	ptc-1	NP_495662.2
Danio rerio (zebrafish)	ptch1	XP_001922161.4
Caenorhabditis elegans (roundworm)	ptc-3	NP_494384.2
Xenopus tropicalis (tropical clawed frog)	ptch1	XP_002935019.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011395	XP_317925.4
Pan troglodytes (chimpanzee)	PTCH1	XP_003312241.1
Bos taurus (cattle)	PTCH1	NP_001192808.1
Caenorhabditis elegans (roundworm)	ptc-2	NP_495218.2
Mus musculus (house mouse)	Ptch1	NP_032983.1
Homo sapiens (human)	PTCH1	NP_000255.2
Drosophila melanogaster (fruit fly)	ptc	NP_523661.2

Gene Ontology
Bibliography

Related articles in PubMed

Localization of
Wan Y, White C, Robert N, Rogers MB, Szabo-Rogers HL
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society67(12)863-871(2019 Dec)

Primary cilia in murine palatal rugae development.
Nakaniwa M, Kawasaki M, Kawasaki K, Yamada A, Meguro F, Takeyasu M, Ohazama A
Gene expression patterns : GEP34119062(2019 12)

Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals.
Yung T, Poon F, Liang M, Coquenlorge S, McGaugh EC, Hui CC, Wilson MD, Nostro MC, Kim TH
Nature communications10(1)4647(2019 10)

Single-Cell Transcriptomics in Medulloblastoma Reveals Tumor-Initiating Progenitors and Oncogenic Cascades during Tumorigenesis and Relapse.
Zhang L, He X, Liu X, Zhang F, Huang LF, Potter AS, Xu L, Zhou W, Zheng T, Luo Z, Berry KP, Pribnow A, Smith SM, Fuller C, Jones BV, Fouladi M, Drissi R, Yang ZJ, Gustafson WC, Remke M, Pomeroy SL, Girard EJ, Olson JM, Morrissy AS, Vladoiu MC, Zhang J, Tian W, Xin M, Taylor MD, Potter SS, Roussel MF, Weiss WA, Lu QR
Cancer cell36(3)302-318.e7(2019 Sep)

The ciliary Frizzled-like receptor Tmem67 regulates canonical Wnt/β-catenin signalling in the developing cerebellum via Hoxb5.
Abdelhamed ZA, Abdelmottaleb DI, El-Asrag ME, Natarajan S, Wheway G, Inglehearn CF, Toomes C, Johnson CA
Scientific reports9(1)5446(2019 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ptch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68507	XM_011244497.2 Latest version!	Mus musculus patched 1 (Ptch1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu22749	NM_008957.3 Latest version!	Mus musculus patched 1 (Ptch1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu48692	NM_001328514.1 Latest version!	Mus musculus patched 1 (Ptch1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu22749	XM_006517159.3 Latest version!	Mus musculus patched 1 (Ptch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu48692	XM_006517160.3 Latest version!	Mus musculus patched 1 (Ptch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu48694	XM_006517162.3 Latest version!	Mus musculus patched 1 (Ptch1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu48694	XM_006517163.3 Latest version!	Mus musculus patched 1 (Ptch1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptch1 ( XM_011244497.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242799.1 Mus musculus patched 1 (Ptch1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu68507
Clone ID Related Accession (Same CDS sequence)	XM_011244497.2
Accession Version	XM_011244497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	protein patched homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG 
CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC 
AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA 
GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT 
GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC 
TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA 
CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA 
GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC 
AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC 
TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG 
AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC 
CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT 
CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG 
GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG 
GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA 
GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC 
TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG 
TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC 
TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC 
CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG 
CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG 
ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA 
GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT 
CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT 
TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT 
GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA 
GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG 
TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC 
TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT 
GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG 
TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC 
CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC 
CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT 
CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA 
CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT 
CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA 
AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC 
TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242799.1
CDS	144..2732
Translation	MVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN

Target ORF information:

RefSeq Version	XM_011244497.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 1 (Ptch1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244497.2

ORF Insert Sequence:

ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG 
CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC 
AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA 
GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT 
GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC 
TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA 
CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA 
GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC 
AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC 
TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG 
AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC 
CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT 
CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG 
GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG 
GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA 
GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC 
TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG 
TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC 
TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC 
CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG 
CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG 
ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA 
GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT 
CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT 
TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT 
GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA 
GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG 
TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC 
TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT 
GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG 
TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC 
CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC 
CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT 
CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA 
CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT 
CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA 
AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC 
TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch1 ( NM_008957.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032983.1 Mus musculus patched 1 (Ptch1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22749
Clone ID Related Accession (Same CDS sequence)	NM_008957.3 , XM_006517159.3
Accession Version	NM_008957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Mus musculus(house mouse)
Product	protein patched homolog 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BF464755.1, AB164615.1, CK639136.1, AK138524.1, CF165613.1, CD545333.1, CK636036.1, CN531162.1, AK163736.1, CO433508.1, CB520013.1, AK147626.1 and BU700582.1. On Jun 16, 2016 this sequence version replaced NM_008957.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U46155.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC 
TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA 
GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT 
CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG 
GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA 
CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT 
CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT 
CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC 
AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT 
TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC 
TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA 
CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA 
GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG 
CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC 
AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT 
ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT 
GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA 
CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC 
ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC 
TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC 
TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT 
TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC 
CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT 
TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT 
GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG 
GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC 
AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC 
CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA 
ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC 
ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC 
AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC 
CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG 
TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG 
GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG 
TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG 
TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC 
CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG 
CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG 
TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT 
ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG 
GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA 
CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA 
AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC 
AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT 
AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC 
GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC 
TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC 
CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG 
AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC 
TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG 
GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG 
ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT 
GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG 
GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG 
GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG 
CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC 
GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC 
TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC 
ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG 
AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC 
AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG 
ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC 
AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC 
CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC 
CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC 
AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC 
ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT 
GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG 
CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT 
GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG 
GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032983.1
CDS	216..4520
Translation	MASAGNAAGALGRQAGGGRRRRTGGPHRAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN

Target ORF information:

RefSeq Version	NM_008957.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 1 (Ptch1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008957.3

ORF Insert Sequence:

ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC 
TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA 
GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT 
CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG 
GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA 
CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT 
CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT 
CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC 
AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT 
TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC 
TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA 
CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA 
GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG 
CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC 
AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT 
ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT 
GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA 
CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC 
ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC 
TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC 
TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT 
TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC 
CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT 
TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT 
GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG 
GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC 
AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC 
CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA 
ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC 
ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC 
AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC 
CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG 
TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG 
GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG 
TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG 
TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC 
CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG 
CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG 
TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT 
ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG 
GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA 
CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA 
AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC 
AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT 
AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC 
GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC 
TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC 
CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG 
AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC 
TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG 
GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG 
ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT 
GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG 
GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG 
GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG 
CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC 
GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC 
TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC 
ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG 
AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC 
AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG 
ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC 
AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC 
CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC 
CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC 
AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC 
ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT 
GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG 
CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT 
GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG 
GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch1 ( NM_001328514.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001315443.1 Mus musculus patched 1 (Ptch1), transcript variant 2, mRNA.

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CloneID	OMu48692
Clone ID Related Accession (Same CDS sequence)	XM_006517160.3 , NM_001328514.1
Accession Version	NM_001328514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Mus musculus(house mouse)
Product	protein patched homolog 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147626.1, AB164615.1, CN535675.1 and BU700582.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC 
CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG 
ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC 
GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT 
CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA 
GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC 
TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA 
TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA 
GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA 
GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC 
ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA 
TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC 
GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC 
TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG 
GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC 
CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC 
CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC 
CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT 
GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA 
CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC 
AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT 
ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG 
CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC 
AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA 
GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT 
GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC 
TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA 
CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA 
GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC 
AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC 
TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG 
AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC 
CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT 
CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG 
GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG 
GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA 
GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC 
TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG 
TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC 
TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC 
CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG 
CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG 
ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA 
GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT 
CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT 
TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT 
GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA 
GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG 
TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC 
TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT 
GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG 
TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC 
CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC 
CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT 
CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA 
CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT 
CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA 
AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC 
TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315443.1
CDS	367..4515
Translation	MGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN

Target ORF information:

RefSeq Version	NM_001328514.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 1 (Ptch1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328514.1

ORF Insert Sequence:

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC 
CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG 
ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC 
GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT 
CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA 
GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC 
TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA 
TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA 
GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA 
GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC 
ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA 
TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC 
GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC 
TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG 
GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC 
CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC 
CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC 
CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT 
GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA 
CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC 
AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT 
ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG 
CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC 
AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA 
GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT 
GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC 
TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA 
CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA 
GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC 
AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC 
TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG 
AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC 
CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT 
CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG 
GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG 
GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA 
GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC 
TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG 
TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC 
TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC 
CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG 
CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG 
ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA 
GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT 
CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT 
TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT 
GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA 
GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG 
TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC 
TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT 
GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG 
TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC 
CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC 
CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT 
CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA 
CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT 
CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA 
AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC 
TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch1 ( XM_006517159.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517222.1 Mus musculus patched 1 (Ptch1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22749
Clone ID Related Accession (Same CDS sequence)	NM_008957.3 , XM_006517159.3
Accession Version	XM_006517159.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	protein patched homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517159.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC 
TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA 
GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT 
CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG 
GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA 
CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT 
CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT 
CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC 
AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT 
TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC 
TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA 
CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA 
GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG 
CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC 
AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT 
ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT 
GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA 
CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC 
ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC 
TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC 
TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT 
TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC 
CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT 
TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT 
GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG 
GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC 
AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC 
CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA 
ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC 
ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC 
AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC 
CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG 
TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG 
GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG 
TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG 
TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC 
CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG 
CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG 
TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT 
ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG 
GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA 
CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA 
AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC 
AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT 
AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC 
GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC 
TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC 
CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG 
AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC 
TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG 
GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG 
ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT 
GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG 
GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG 
GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG 
CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC 
GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC 
TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC 
ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG 
AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC 
AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG 
ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC 
AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC 
CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC 
CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC 
AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC 
ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT 
GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG 
CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT 
GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG 
GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517222.1
CDS	298..4602
Translation	MASAGNAAGALGRQAGGGRRRRTGGPHRAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN

Target ORF information:

RefSeq Version	XM_006517159.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 1 (Ptch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517159.3

ORF Insert Sequence:

ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC 
TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA 
GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT 
CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG 
GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA 
CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT 
CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT 
CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC 
AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT 
TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC 
TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA 
CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA 
GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG 
CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC 
AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT 
ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT 
GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA 
CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC 
ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC 
TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC 
TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT 
TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC 
CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT 
TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT 
GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG 
GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC 
AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC 
CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA 
ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC 
ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC 
AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC 
CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG 
TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG 
GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG 
TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG 
TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC 
CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG 
CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG 
TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT 
ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG 
GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA 
CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA 
AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC 
AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT 
AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC 
GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC 
TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC 
CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG 
AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC 
TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG 
GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG 
ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT 
GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG 
GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG 
GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG 
CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC 
GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC 
TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC 
ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG 
AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC 
AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG 
ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC 
AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC 
CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC 
CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC 
AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC 
ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT 
GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG 
CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT 
GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG 
GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch1 ( XM_006517160.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517223.1 Mus musculus patched 1 (Ptch1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu48692
Clone ID Related Accession (Same CDS sequence)	XM_006517160.3 , NM_001328514.1
Accession Version	XM_006517160.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	protein patched homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517160.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC 
CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG 
ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC 
GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT 
CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA 
GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC 
TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA 
TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA 
GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA 
GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC 
ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA 
TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC 
GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC 
TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG 
GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC 
CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC 
CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC 
CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT 
GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA 
CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC 
AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT 
ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG 
CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC 
AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA 
GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT 
GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC 
TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA 
CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA 
GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC 
AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC 
TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG 
AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC 
CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT 
CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG 
GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG 
GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA 
GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC 
TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG 
TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC 
TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC 
CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG 
CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG 
ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA 
GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT 
CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT 
TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT 
GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA 
GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG 
TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC 
TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT 
GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG 
TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC 
CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC 
CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT 
CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA 
CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT 
CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA 
AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC 
TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517223.1
CDS	523..4671
Translation	MGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN

Target ORF information:

RefSeq Version	XM_006517160.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 1 (Ptch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517160.3

ORF Insert Sequence:

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC 
CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG 
ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC 
GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT 
CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA 
GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC 
TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA 
TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA 
GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA 
GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC 
ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA 
TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC 
GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC 
TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG 
GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC 
CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC 
CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC 
CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT 
GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA 
CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC 
AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT 
ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG 
CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC 
AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA 
GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT 
GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC 
TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA 
CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA 
GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC 
AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC 
TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG 
AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC 
CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT 
CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG 
GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG 
GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA 
GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC 
TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG 
TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC 
TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC 
CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG 
CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG 
ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA 
GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT 
CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT 
TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT 
GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA 
GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG 
TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC 
TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT 
GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG 
TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC 
CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC 
CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT 
CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA 
CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT 
CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA 
AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC 
TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch1 ( XM_006517163.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517226.1 Mus musculus patched 1 (Ptch1), transcript variant X4, mRNA.

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CloneID	OMu48694
Clone ID Related Accession (Same CDS sequence)	XM_006517163.3 , XM_006517162.3
Accession Version	XM_006517163.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	protein patched homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517163.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC 
ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG 
GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG 
CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA 
AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC 
ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA 
AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC 
AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT 
GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG 
GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC 
GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA 
AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC 
TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT 
GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT 
GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT 
CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC 
TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA 
GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC 
TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA 
GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC 
CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC 
ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC 
GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG 
CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA 
CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT 
GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG 
AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA 
GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT 
GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG 
CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC 
AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC 
AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT 
GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT 
GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC 
AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC 
GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA 
GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT 
GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC 
ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG 
ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG 
GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC 
ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG 
AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG 
TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC 
TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG 
CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC 
CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT 
GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG 
TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC 
CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT 
CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC 
TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG 
CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC 
CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG 
GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA 
GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517226.1
CDS	429..4322
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN

Target ORF information:

RefSeq Version	XM_006517163.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 1 (Ptch1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517163.3

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC 
ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG 
GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG 
CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA 
AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC 
ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA 
AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC 
AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT 
GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG 
GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC 
GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA 
AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC 
TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT 
GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT 
GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT 
CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC 
TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA 
GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC 
TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA 
GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC 
CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC 
ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC 
GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG 
CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA 
CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT 
GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG 
AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA 
GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT 
GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG 
CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC 
AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC 
AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT 
GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT 
GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC 
AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC 
GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA 
GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT 
GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC 
ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG 
ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG 
GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC 
ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG 
AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG 
TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC 
TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG 
CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC 
CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT 
GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG 
TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC 
CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT 
CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC 
TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG 
CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC 
CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG 
GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA 
GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch1 ( XM_006517162.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517225.1 Mus musculus patched 1 (Ptch1), transcript variant X3, mRNA.

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CloneID	OMu48694
Clone ID Related Accession (Same CDS sequence)	XM_006517163.3 , XM_006517162.3
Accession Version	XM_006517162.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	protein patched homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517162.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC 
ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG 
GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG 
CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA 
AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC 
ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA 
AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC 
AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT 
GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG 
GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC 
GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA 
AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC 
TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT 
GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT 
GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT 
CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC 
TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA 
GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC 
TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA 
GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC 
CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC 
ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC 
GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG 
CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA 
CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT 
GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG 
AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA 
GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT 
GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG 
CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC 
AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC 
AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT 
GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT 
GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC 
AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC 
GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA 
GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT 
GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC 
ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG 
ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG 
GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC 
ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG 
AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG 
TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC 
TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG 
CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC 
CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT 
GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG 
TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC 
CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT 
CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC 
TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG 
CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC 
CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG 
GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA 
GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517225.1
CDS	163..4056
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPPYTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSVVRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDSRHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGARSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPHVPFHVRCERRDSKVEVIELQDVECEERPWGSSSN

Target ORF information:

RefSeq Version	XM_006517162.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 1 (Ptch1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517162.3

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC 
ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG 
GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG 
CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA 
AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC 
ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA 
AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC 
AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT 
GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG 
GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC 
GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA 
AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC 
TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT 
GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT 
GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT 
CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC 
TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA 
GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC 
TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA 
GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC 
CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC 
ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC 
GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG 
CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA 
CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT 
GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG 
AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA 
GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT 
GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG 
CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC 
AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC 
AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT 
GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT 
GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC 
AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC 
GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA 
GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT 
GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC 
ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG 
ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG 
GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC 
ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG 
AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG 
TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC 
TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG 
CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC 
CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT 
GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG 
TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC 
CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT 
CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC 
TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG 
CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC 
CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG 
GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA 
GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Localization of
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society67(12)863-871(2019 Dec)
Wan Y,White C,Robert N,Rogers MB,Szabo-Rogers HL

Primary cilia in murine palatal rugae development.
Gene expression patterns : GEP34119062(2019 12)
Nakaniwa M,Kawasaki M,Kawasaki K,Yamada A,Meguro F,Takeyasu M,Ohazama A

Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals.
Nature communications10(1)4647(2019 10)
Yung T,Poon F,Liang M,Coquenlorge S,McGaugh EC,Hui CC,Wilson MD,Nostro MC,Kim TH

Single-Cell Transcriptomics in Medulloblastoma Reveals Tumor-Initiating Progenitors and Oncogenic Cascades during Tumorigenesis and Relapse.
Cancer cell36(3)302-318.e7(2019 Sep)
Zhang L,He X,Liu X,Zhang F,Huang LF,Potter AS,Xu L,Zhou W,Zheng T,Luo Z,Berry KP,Pribnow A,Smith SM,Fuller C,Jones BV,Fouladi M,Drissi R,Yang ZJ,Gustafson WC,Remke M,Pomeroy SL,Girard EJ,Olson JM,Morrissy AS,Vladoiu MC,Zhang J,Tian W,Xin M,Taylor MD,Potter SS,Roussel MF,Weiss WA,Lu QR

The ciliary Frizzled-like receptor Tmem67 regulates canonical Wnt/β-catenin signalling in the developing cerebellum via Hoxb5.
Scientific reports9(1)5446(2019 Apr)
Abdelhamed ZA,Abdelmottaleb DI,El-Asrag ME,Natarajan S,Wheway G,Inglehearn CF,Toomes C,Johnson CA

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Ptch1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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