ARHGEF12 cDNA ORF clone, Felis catus(domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF12
Entrez Gene ID	101085657
Full Name	Rho guanine nucleotide exchange factor 12
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006936730.4	XP_006936792.1	rho guanine nucleotide exchange factor 12 isoform X1
XM_006936730.3	XP_006936792.1	rho guanine nucleotide exchange factor 12 isoform X1
XM_023239193.1	XP_023094961.1	rho guanine nucleotide exchange factor 12 isoform X4
XM_019811430.2	XP_019666989.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_006936731.4	XP_006936793.1	rho guanine nucleotide exchange factor 12 isoform X2
XM_019811430.1	XP_019666989.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_006936731.3	XP_006936793.1	rho guanine nucleotide exchange factor 12 isoform X2
XM_023239192.1	XP_023094960.1	rho guanine nucleotide exchange factor 12 isoform X4

Pathways from BioSystems
Gene Ontology

The following ARHGEF12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb60757	XM_023239193.1 Latest version!	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb60757	XM_023239192.1 Latest version!	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb14134	XM_006936730.4 Latest version!	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb14135	XM_006936731.4 Latest version!	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb49425	XM_019811430.2 Latest version!	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OFb14134	XM_006936730.3	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb49425	XM_019811430.1	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb14135	XM_006936731.3	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF12 ( XM_023239193.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023094961.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OFb60757
Clone ID Related Accession (Same CDS sequence)	XM_023239193.1 , XM_023239192.1
Accession Version	XM_023239193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGTAC ATTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGATTCTGA AGTAGAGTCA 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG AGCATCTGGG 
AACATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AAGAACGGCT GCAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CGTCCTCCAG GTCTTTAGGT GACCCCCCGA CAGTCAGTGA GGTAGAAGCA 
GACCCTGGTG ACGGACTAGG GAGAATTGAC TACAGCAGCG GAGATGCTTC TCGGCCCAGT 
AGTGACAGCG CGGAAAGTCC CAAGAGTGCC CTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAGAAAA GCGAAGTGAT TCAGGACACT GACACTCAGT CACTCATCGG GAGCCCCTCT 
CCCCGAATGG CACCTCATAT TATTGGAGCA GAGGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTCCAG AGCATTGAAC TGCTAAAATC TCGCCCTGCT 
CACCTGGCTG TTTTCTTACA CCACGTAGTT TCACAGTTTG ACCCTGCAAC GTTGCTCTGT 
TATCTCTATT CAGACCTATA TAAGCAGACC AATTCCAAGG AGACTCGGCG TGTCTTCCTC 
GAGTTTCATC AGTTTTTCCT GGATCGATCT GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATATCAATAG ATCTAGAAAA AAGAAGACCT GAGCTTATAC CTGAGGATCT ACACCGCCAC 
TATATTCAAA CTATGCAGGA AAGAGTCCAC CCAGAAGTTC AGAGGCACTT GGAAGATTTT 
CGGCAGAAAC GTAGCATGGG GCTGACCTTG GCTGAAAGCG AGCTGACAAA ACTCGATGCA 
GAGCGAGACA AGGACCGGGT AACTTTGGAG AAGGAACGGG CATGTGCGGA ACAGATTGTT 
GCCAAAATCG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGATAA AAGTTCAACA 
ATGCAGTATG TTATCCTTAT GTACATGAAG CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATCGAG AAGGCGAAGA AAAAGGGAAG CGCAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCAAGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCTATGGA ACTACAGAAG 
CAGCGGCACC CTAAGCACTT ATCCACACCC TCTTCTGTGA GTCCTGAGCC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAGGT GAAGGAACAG ACGTTGGGTA CCTGCCAGCC 
AATTCCATGT CCTCTGGGGC TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATCTGGGAT CAAAGCAAGT TGGAGAAACA CCTGCATCCG GAGACTGCAT GGATGGCGCC 
CCTCGCACTC CCAATACCAT CTTCGATTTC CCACCTCCTC CGTTAGACCA AGTGCAGGAG 
GAAGAGTGGG AGGTGGAAAG GGTGCCTGAA CATGGGACAC CAAAGCCCTT TCGAAAATTC 
GACAGCATAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA ACAGCTCGTT AGTCGAGAAG TGTTACTGGG ACTAAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TACCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCTTCAG AACTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCAGTTCGC AAGAGGAATG AGACCTCAGT TATTGATCAG 
ATTGGGGAAG ATTTACTGAC CTGGTTCAGC GGACCAGGGG AGGAGAAGTT GAAACACGCT 
GCTGCCACCT TTTGCAGTAA CCAACCTTTT GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCCC GATTTCAGAC TTTTGTGCAA GATGCCGAGA GCAATCCGCT GTGTCGTCGT 
CTGCAGCTGA AGGATATCAT TCCTACCCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTG 
TTAGATAATA TTGCCAAATA CACAGAATGG CCAATGGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAACTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAT 
AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTCGATACCT CCAATCTGAA GTTGTCAGAG 
TACCCAAATG TGGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCACTGG TCTGGAAGGT GAATCGGGAT AAAACTATCG ATCTGTACAC GTTGCTGCTG 
GAAGACATTC TGGTATTGTT ACAAAAACAG GATGACAGAC TGGTGTTACG ATGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAAT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TGTTTGTCAT CTCCATGTCG 
GACAATGGGG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTTA TCTGCCGGAT GGCCGCATCA GTGAAGGAAC AGTCGGCGAA GCCAATCCCA 
CTGCCCCAGT CACCACCTTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAGCATC ATAGCATTTC AGTCCCTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGGGTGCCTC TGGGAAGTCC 
GAGGTAGATG ATCTGTTTGT GGCTGAGAGA CAGTTTGCTA AGGAGCGGCA TGCAGATGGG 
ACTCTGCAGG AAGCTGGAGC AAGTTACCAA ATTCCAATCC CAGACCCGCA CTTGCCTGCC 
TCAGAAGAAC GGTGGGCCTT GGACGCACTA AGGAATTTGG GCTTGTTGAA GCAGTTGCTT 
GTACAACAGC TGGGTTTGGC TGAGAAGAGC ACTCAGGAAG ACTGGTCACG TTTCCTGAGA 
TACGGAACAG CGTCTTCCGG GGTGCAGACA GACAGTGTAA CCCAGAACTC TGAAAATAGT 
AAGGCCTGTC CTCCTGGTGA AGGACAGGTG TCCTTTAGAA CTGAAAGTGG TGACATTTCC 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGAGATTC AGTGGTACTG 
GCATTCCAAG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CTGCAGAGCC AGAAGGGAGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGGGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGTGGT TAAGAAGGAA 
GAGGATGTTA ATTTGCGCAT CTCAGGAAAC TATTTGATCC TTGACGGCTA CGATGCGGTG 
CAGGAGAGCT CCACGGATGA GGAGGTTGCT TCCTCACTCC CCCCACCGCC CACGACAGGC 
GCCCCCCCCA CGGACTCTGC CGGCCAGCAG CAGCATTCTC CCCAGAACGC CCATTCCGAT 
GGGGCGCTCT CACCGTTCAC CCCGGAATTC CTGGTCCAGC AGCGCTGGGG AGCTATGGAG 
GATTCCTGTT TTGAGATCCA GAGTCCCCCC TCTTGTGCAG ACTCACAAAG CCAGATCATG 
GAGTACATTC GTAAGATCGA GGCTGACCTT GAACACTTAA AGAAGGTGGA GGAAAGTTAC 
AACATTCTTT GCCAAAGGCT GGCTGGATCA GCCCTCACAG ACAAGCACTC AGATAAAAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023094961.1
CDS	239..4561
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_023239193.1
Organism	Felis catus(domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023239193.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGTAC ATTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGATTCTGA AGTAGAGTCA 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG AGCATCTGGG 
AACATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AAGAACGGCT GCAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CGTCCTCCAG GTCTTTAGGT GACCCCCCGA CAGTCAGTGA GGTAGAAGCA 
GACCCTGGTG ACGGACTAGG GAGAATTGAC TACAGCAGCG GAGATGCTTC TCGGCCCAGT 
AGTGACAGCG CGGAAAGTCC CAAGAGTGCC CTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAGAAAA GCGAAGTGAT TCAGGACACT GACACTCAGT CACTCATCGG GAGCCCCTCT 
CCCCGAATGG CACCTCATAT TATTGGAGCA GAGGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTCCAG AGCATTGAAC TGCTAAAATC TCGCCCTGCT 
CACCTGGCTG TTTTCTTACA CCACGTAGTT TCACAGTTTG ACCCTGCAAC GTTGCTCTGT 
TATCTCTATT CAGACCTATA TAAGCAGACC AATTCCAAGG AGACTCGGCG TGTCTTCCTC 
GAGTTTCATC AGTTTTTCCT GGATCGATCT GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATATCAATAG ATCTAGAAAA AAGAAGACCT GAGCTTATAC CTGAGGATCT ACACCGCCAC 
TATATTCAAA CTATGCAGGA AAGAGTCCAC CCAGAAGTTC AGAGGCACTT GGAAGATTTT 
CGGCAGAAAC GTAGCATGGG GCTGACCTTG GCTGAAAGCG AGCTGACAAA ACTCGATGCA 
GAGCGAGACA AGGACCGGGT AACTTTGGAG AAGGAACGGG CATGTGCGGA ACAGATTGTT 
GCCAAAATCG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGATAA AAGTTCAACA 
ATGCAGTATG TTATCCTTAT GTACATGAAG CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATCGAG AAGGCGAAGA AAAAGGGAAG CGCAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCAAGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCTATGGA ACTACAGAAG 
CAGCGGCACC CTAAGCACTT ATCCACACCC TCTTCTGTGA GTCCTGAGCC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAGGT GAAGGAACAG ACGTTGGGTA CCTGCCAGCC 
AATTCCATGT CCTCTGGGGC TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATCTGGGAT CAAAGCAAGT TGGAGAAACA CCTGCATCCG GAGACTGCAT GGATGGCGCC 
CCTCGCACTC CCAATACCAT CTTCGATTTC CCACCTCCTC CGTTAGACCA AGTGCAGGAG 
GAAGAGTGGG AGGTGGAAAG GGTGCCTGAA CATGGGACAC CAAAGCCCTT TCGAAAATTC 
GACAGCATAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA ACAGCTCGTT AGTCGAGAAG TGTTACTGGG ACTAAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TACCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCTTCAG AACTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCAGTTCGC AAGAGGAATG AGACCTCAGT TATTGATCAG 
ATTGGGGAAG ATTTACTGAC CTGGTTCAGC GGACCAGGGG AGGAGAAGTT GAAACACGCT 
GCTGCCACCT TTTGCAGTAA CCAACCTTTT GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCCC GATTTCAGAC TTTTGTGCAA GATGCCGAGA GCAATCCGCT GTGTCGTCGT 
CTGCAGCTGA AGGATATCAT TCCTACCCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTG 
TTAGATAATA TTGCCAAATA CACAGAATGG CCAATGGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAACTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAT 
AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTCGATACCT CCAATCTGAA GTTGTCAGAG 
TACCCAAATG TGGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCACTGG TCTGGAAGGT GAATCGGGAT AAAACTATCG ATCTGTACAC GTTGCTGCTG 
GAAGACATTC TGGTATTGTT ACAAAAACAG GATGACAGAC TGGTGTTACG ATGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAAT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TGTTTGTCAT CTCCATGTCG 
GACAATGGGG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTTA TCTGCCGGAT GGCCGCATCA GTGAAGGAAC AGTCGGCGAA GCCAATCCCA 
CTGCCCCAGT CACCACCTTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAGCATC ATAGCATTTC AGTCCCTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGGGTGCCTC TGGGAAGTCC 
GAGGTAGATG ATCTGTTTGT GGCTGAGAGA CAGTTTGCTA AGGAGCGGCA TGCAGATGGG 
ACTCTGCAGG AAGCTGGAGC AAGTTACCAA ATTCCAATCC CAGACCCGCA CTTGCCTGCC 
TCAGAAGAAC GGTGGGCCTT GGACGCACTA AGGAATTTGG GCTTGTTGAA GCAGTTGCTT 
GTACAACAGC TGGGTTTGGC TGAGAAGAGC ACTCAGGAAG ACTGGTCACG TTTCCTGAGA 
TACGGAACAG CGTCTTCCGG GGTGCAGACA GACAGTGTAA CCCAGAACTC TGAAAATAGT 
AAGGCCTGTC CTCCTGGTGA AGGACAGGTG TCCTTTAGAA CTGAAAGTGG TGACATTTCC 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGAGATTC AGTGGTACTG 
GCATTCCAAG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CTGCAGAGCC AGAAGGGAGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGGGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGTGGT TAAGAAGGAA 
GAGGATGTTA ATTTGCGCAT CTCAGGAAAC TATTTGATCC TTGACGGCTA CGATGCGGTG 
CAGGAGAGCT CCACGGATGA GGAGGTTGCT TCCTCACTCC CCCCACCGCC CACGACAGGC 
GCCCCCCCCA CGGACTCTGC CGGCCAGCAG CAGCATTCTC CCCAGAACGC CCATTCCGAT 
GGGGCGCTCT CACCGTTCAC CCCGGAATTC CTGGTCCAGC AGCGCTGGGG AGCTATGGAG 
GATTCCTGTT TTGAGATCCA GAGTCCCCCC TCTTGTGCAG ACTCACAAAG CCAGATCATG 
GAGTACATTC GTAAGATCGA GGCTGACCTT GAACACTTAA AGAAGGTGGA GGAAAGTTAC 
AACATTCTTT GCCAAAGGCT GGCTGGATCA GCCCTCACAG ACAAGCACTC AGATAAAAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_023239192.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023094960.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFb60757
Clone ID Related Accession (Same CDS sequence)	XM_023239193.1 , XM_023239192.1
Accession Version	XM_023239192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGTAC ATTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGATTCTGA AGTAGAGTCA 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG AGCATCTGGG 
AACATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AAGAACGGCT GCAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CGTCCTCCAG GTCTTTAGGT GACCCCCCGA CAGTCAGTGA GGTAGAAGCA 
GACCCTGGTG ACGGACTAGG GAGAATTGAC TACAGCAGCG GAGATGCTTC TCGGCCCAGT 
AGTGACAGCG CGGAAAGTCC CAAGAGTGCC CTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAGAAAA GCGAAGTGAT TCAGGACACT GACACTCAGT CACTCATCGG GAGCCCCTCT 
CCCCGAATGG CACCTCATAT TATTGGAGCA GAGGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTCCAG AGCATTGAAC TGCTAAAATC TCGCCCTGCT 
CACCTGGCTG TTTTCTTACA CCACGTAGTT TCACAGTTTG ACCCTGCAAC GTTGCTCTGT 
TATCTCTATT CAGACCTATA TAAGCAGACC AATTCCAAGG AGACTCGGCG TGTCTTCCTC 
GAGTTTCATC AGTTTTTCCT GGATCGATCT GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATATCAATAG ATCTAGAAAA AAGAAGACCT GAGCTTATAC CTGAGGATCT ACACCGCCAC 
TATATTCAAA CTATGCAGGA AAGAGTCCAC CCAGAAGTTC AGAGGCACTT GGAAGATTTT 
CGGCAGAAAC GTAGCATGGG GCTGACCTTG GCTGAAAGCG AGCTGACAAA ACTCGATGCA 
GAGCGAGACA AGGACCGGGT AACTTTGGAG AAGGAACGGG CATGTGCGGA ACAGATTGTT 
GCCAAAATCG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGATAA AAGTTCAACA 
ATGCAGTATG TTATCCTTAT GTACATGAAG CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATCGAG AAGGCGAAGA AAAAGGGAAG CGCAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCAAGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCTATGGA ACTACAGAAG 
CAGCGGCACC CTAAGCACTT ATCCACACCC TCTTCTGTGA GTCCTGAGCC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAGGT GAAGGAACAG ACGTTGGGTA CCTGCCAGCC 
AATTCCATGT CCTCTGGGGC TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATCTGGGAT CAAAGCAAGT TGGAGAAACA CCTGCATCCG GAGACTGCAT GGATGGCGCC 
CCTCGCACTC CCAATACCAT CTTCGATTTC CCACCTCCTC CGTTAGACCA AGTGCAGGAG 
GAAGAGTGGG AGGTGGAAAG GGTGCCTGAA CATGGGACAC CAAAGCCCTT TCGAAAATTC 
GACAGCATAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA ACAGCTCGTT AGTCGAGAAG TGTTACTGGG ACTAAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TACCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCTTCAG AACTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCAGTTCGC AAGAGGAATG AGACCTCAGT TATTGATCAG 
ATTGGGGAAG ATTTACTGAC CTGGTTCAGC GGACCAGGGG AGGAGAAGTT GAAACACGCT 
GCTGCCACCT TTTGCAGTAA CCAACCTTTT GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCCC GATTTCAGAC TTTTGTGCAA GATGCCGAGA GCAATCCGCT GTGTCGTCGT 
CTGCAGCTGA AGGATATCAT TCCTACCCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTG 
TTAGATAATA TTGCCAAATA CACAGAATGG CCAATGGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAACTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAT 
AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTCGATACCT CCAATCTGAA GTTGTCAGAG 
TACCCAAATG TGGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCACTGG TCTGGAAGGT GAATCGGGAT AAAACTATCG ATCTGTACAC GTTGCTGCTG 
GAAGACATTC TGGTATTGTT ACAAAAACAG GATGACAGAC TGGTGTTACG ATGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAAT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TGTTTGTCAT CTCCATGTCG 
GACAATGGGG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTTA TCTGCCGGAT GGCCGCATCA GTGAAGGAAC AGTCGGCGAA GCCAATCCCA 
CTGCCCCAGT CACCACCTTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAGCATC ATAGCATTTC AGTCCCTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGGGTGCCTC TGGGAAGTCC 
GAGGTAGATG ATCTGTTTGT GGCTGAGAGA CAGTTTGCTA AGGAGCGGCA TGCAGATGGG 
ACTCTGCAGG AAGCTGGAGC AAGTTACCAA ATTCCAATCC CAGACCCGCA CTTGCCTGCC 
TCAGAAGAAC GGTGGGCCTT GGACGCACTA AGGAATTTGG GCTTGTTGAA GCAGTTGCTT 
GTACAACAGC TGGGTTTGGC TGAGAAGAGC ACTCAGGAAG ACTGGTCACG TTTCCTGAGA 
TACGGAACAG CGTCTTCCGG GGTGCAGACA GACAGTGTAA CCCAGAACTC TGAAAATAGT 
AAGGCCTGTC CTCCTGGTGA AGGACAGGTG TCCTTTAGAA CTGAAAGTGG TGACATTTCC 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGAGATTC AGTGGTACTG 
GCATTCCAAG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CTGCAGAGCC AGAAGGGAGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGGGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGTGGT TAAGAAGGAA 
GAGGATGTTA ATTTGCGCAT CTCAGGAAAC TATTTGATCC TTGACGGCTA CGATGCGGTG 
CAGGAGAGCT CCACGGATGA GGAGGTTGCT TCCTCACTCC CCCCACCGCC CACGACAGGC 
GCCCCCCCCA CGGACTCTGC CGGCCAGCAG CAGCATTCTC CCCAGAACGC CCATTCCGAT 
GGGGCGCTCT CACCGTTCAC CCCGGAATTC CTGGTCCAGC AGCGCTGGGG AGCTATGGAG 
GATTCCTGTT TTGAGATCCA GAGTCCCCCC TCTTGTGCAG ACTCACAAAG CCAGATCATG 
GAGTACATTC GTAAGATCGA GGCTGACCTT GAACACTTAA AGAAGGTGGA GGAAAGTTAC 
AACATTCTTT GCCAAAGGCT GGCTGGATCA GCCCTCACAG ACAAGCACTC AGATAAAAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023094960.1
CDS	296..4618
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_023239192.1
Organism	Felis catus(domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023239192.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGTAC ATTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGATTCTGA AGTAGAGTCA 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG AGCATCTGGG 
AACATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AAGAACGGCT GCAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CGTCCTCCAG GTCTTTAGGT GACCCCCCGA CAGTCAGTGA GGTAGAAGCA 
GACCCTGGTG ACGGACTAGG GAGAATTGAC TACAGCAGCG GAGATGCTTC TCGGCCCAGT 
AGTGACAGCG CGGAAAGTCC CAAGAGTGCC CTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAGAAAA GCGAAGTGAT TCAGGACACT GACACTCAGT CACTCATCGG GAGCCCCTCT 
CCCCGAATGG CACCTCATAT TATTGGAGCA GAGGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTCCAG AGCATTGAAC TGCTAAAATC TCGCCCTGCT 
CACCTGGCTG TTTTCTTACA CCACGTAGTT TCACAGTTTG ACCCTGCAAC GTTGCTCTGT 
TATCTCTATT CAGACCTATA TAAGCAGACC AATTCCAAGG AGACTCGGCG TGTCTTCCTC 
GAGTTTCATC AGTTTTTCCT GGATCGATCT GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATATCAATAG ATCTAGAAAA AAGAAGACCT GAGCTTATAC CTGAGGATCT ACACCGCCAC 
TATATTCAAA CTATGCAGGA AAGAGTCCAC CCAGAAGTTC AGAGGCACTT GGAAGATTTT 
CGGCAGAAAC GTAGCATGGG GCTGACCTTG GCTGAAAGCG AGCTGACAAA ACTCGATGCA 
GAGCGAGACA AGGACCGGGT AACTTTGGAG AAGGAACGGG CATGTGCGGA ACAGATTGTT 
GCCAAAATCG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGATAA AAGTTCAACA 
ATGCAGTATG TTATCCTTAT GTACATGAAG CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATCGAG AAGGCGAAGA AAAAGGGAAG CGCAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCAAGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCTATGGA ACTACAGAAG 
CAGCGGCACC CTAAGCACTT ATCCACACCC TCTTCTGTGA GTCCTGAGCC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAGGT GAAGGAACAG ACGTTGGGTA CCTGCCAGCC 
AATTCCATGT CCTCTGGGGC TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATCTGGGAT CAAAGCAAGT TGGAGAAACA CCTGCATCCG GAGACTGCAT GGATGGCGCC 
CCTCGCACTC CCAATACCAT CTTCGATTTC CCACCTCCTC CGTTAGACCA AGTGCAGGAG 
GAAGAGTGGG AGGTGGAAAG GGTGCCTGAA CATGGGACAC CAAAGCCCTT TCGAAAATTC 
GACAGCATAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA ACAGCTCGTT AGTCGAGAAG TGTTACTGGG ACTAAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TACCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCTTCAG AACTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCAGTTCGC AAGAGGAATG AGACCTCAGT TATTGATCAG 
ATTGGGGAAG ATTTACTGAC CTGGTTCAGC GGACCAGGGG AGGAGAAGTT GAAACACGCT 
GCTGCCACCT TTTGCAGTAA CCAACCTTTT GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCCC GATTTCAGAC TTTTGTGCAA GATGCCGAGA GCAATCCGCT GTGTCGTCGT 
CTGCAGCTGA AGGATATCAT TCCTACCCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTG 
TTAGATAATA TTGCCAAATA CACAGAATGG CCAATGGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAACTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAT 
AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTCGATACCT CCAATCTGAA GTTGTCAGAG 
TACCCAAATG TGGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCACTGG TCTGGAAGGT GAATCGGGAT AAAACTATCG ATCTGTACAC GTTGCTGCTG 
GAAGACATTC TGGTATTGTT ACAAAAACAG GATGACAGAC TGGTGTTACG ATGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAAT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TGTTTGTCAT CTCCATGTCG 
GACAATGGGG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTTA TCTGCCGGAT GGCCGCATCA GTGAAGGAAC AGTCGGCGAA GCCAATCCCA 
CTGCCCCAGT CACCACCTTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAGCATC ATAGCATTTC AGTCCCTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGGGTGCCTC TGGGAAGTCC 
GAGGTAGATG ATCTGTTTGT GGCTGAGAGA CAGTTTGCTA AGGAGCGGCA TGCAGATGGG 
ACTCTGCAGG AAGCTGGAGC AAGTTACCAA ATTCCAATCC CAGACCCGCA CTTGCCTGCC 
TCAGAAGAAC GGTGGGCCTT GGACGCACTA AGGAATTTGG GCTTGTTGAA GCAGTTGCTT 
GTACAACAGC TGGGTTTGGC TGAGAAGAGC ACTCAGGAAG ACTGGTCACG TTTCCTGAGA 
TACGGAACAG CGTCTTCCGG GGTGCAGACA GACAGTGTAA CCCAGAACTC TGAAAATAGT 
AAGGCCTGTC CTCCTGGTGA AGGACAGGTG TCCTTTAGAA CTGAAAGTGG TGACATTTCC 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGAGATTC AGTGGTACTG 
GCATTCCAAG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CTGCAGAGCC AGAAGGGAGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGGGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGTGGT TAAGAAGGAA 
GAGGATGTTA ATTTGCGCAT CTCAGGAAAC TATTTGATCC TTGACGGCTA CGATGCGGTG 
CAGGAGAGCT CCACGGATGA GGAGGTTGCT TCCTCACTCC CCCCACCGCC CACGACAGGC 
GCCCCCCCCA CGGACTCTGC CGGCCAGCAG CAGCATTCTC CCCAGAACGC CCATTCCGAT 
GGGGCGCTCT CACCGTTCAC CCCGGAATTC CTGGTCCAGC AGCGCTGGGG AGCTATGGAG 
GATTCCTGTT TTGAGATCCA GAGTCCCCCC TCTTGTGCAG ACTCACAAAG CCAGATCATG 
GAGTACATTC GTAAGATCGA GGCTGACCTT GAACACTTAA AGAAGGTGGA GGAAAGTTAC 
AACATTCTTT GCCAAAGGCT GGCTGGATCA GCCCTCACAG ACAAGCACTC AGATAAAAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_006936730.4 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_006936792.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

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CloneID	OFb14134
Clone ID Related Accession (Same CDS sequence)	XM_006936730.4 , XM_006936730.3
Accession Version	XM_006936730.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_006936730.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006936792.1
CDS	712..5343
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_006936730.4
Organism	Felis catus(domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006936730.4

ORF Insert Sequence:

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_006936730.3 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_006936792.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

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CloneID	OFb14134
Clone ID Related Accession (Same CDS sequence)	XM_006936730.4 , XM_006936730.3
Accession Version	XM_006936730.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006936730.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006936792.1
CDS	713..5344
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_006936730.3
Organism	Felis catus(Domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006936730.3

ORF Insert Sequence:

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_006936731.4 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_006936793.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb14135
Clone ID Related Accession (Same CDS sequence)	XM_006936731.4 , XM_006936731.3
Accession Version	XM_006936731.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_006936731.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGGTCTTGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGT 
ACATTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGATTCT 
GAAGTAGAGT CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT 
GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAAGAACGG 
CTGCAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACGTCCTCC AGGTCTTTAG GTGACCCCCC GACAGTCAGT 
GAGGTAGAAG CAGACCCTGG TGACGGACTA GGGAGAATTG ACTACAGCAG CGGAGATGCT 
TCTCGGCCCA GTAGTGACAG CGCGGAAAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGCGAAGTG ATTCAGGACA CTGACACTCA GTCACTCATC 
GGGAGCCCCT CTCCCCGAAT GGCACCTCAT ATTATTGGAG CAGAGGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTAAAA 
TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCACGTAG TTTCACAGTT TGACCCTGCA 
ACGTTGCTCT GTTATCTCTA TTCAGACCTA TATAAGCAGA CCAATTCCAA GGAGACTCGG 
CGTGTCTTCC TCGAGTTTCA TCAGTTTTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATATCAAT AGATCTAGAA AAAAGAAGAC CTGAGCTTAT ACCTGAGGAT 
CTACACCGCC ACTATATTCA AACTATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC 
TTGGAAGATT TTCGGCAGAA ACGTAGCATG GGGCTGACCT TGGCTGAAAG CGAGCTGACA 
AAACTCGATG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCG 
GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAT 
AAAAGTTCAA CAATGCAGTA TGTTATCCTT ATGTACATGA AGCATTTGGG AGTAAAAGTG 
AAAGAACCTC GAAATTTGGA GCACAAGCGA GGTCGGATTG GATTCCTTCC AAAAATCAAG 
CAAAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGGA AGCGCAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCTATG 
GAACTACAGA AGCAGCGGCA CCCTAAGCAC TTATCCACAC CCTCTTCTGT GAGTCCTGAG 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAG GTGAAGGAAC AGACGTTGGG 
TACCTGCCAG CCAATTCCAT GTCCTCTGGG GCTTCAGGGG CCACTCTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATCTGGG ATCAAAGCAA GTTGGAGAAA CACCTGCATC CGGAGACTGC 
ATGGATGGCG CCCCTCGCAC TCCCAATACC ATCTTCGATT TCCCACCTCC TCCGTTAGAC 
CAAGTGCAGG AGGAAGAGTG GGAGGTGGAA AGGGTGCCTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAAT TCGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG 
GGACTAAAGC CTTGTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTACCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCTTC AGAACTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCAGTTC GCAAGAGGAA TGAGACCTCA 
GTTATTGATC AGATTGGGGA AGATTTACTG ACCTGGTTCA GCGGACCAGG GGAGGAGAAG 
TTGAAACACG CTGCTGCCAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC CCGATTTCAG ACTTTTGTGC AAGATGCCGA GAGCAATCCG 
CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AAATGCAGAG GCTTACTAAG 
TACCCTCTTC TGTTAGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAACT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ATAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTCGATAC CTCCAATCTG 
AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCACT GGTCTGGAAG GTGAATCGGG ATAAAACTAT CGATCTGTAC 
ACGTTGCTGC TGGAAGACAT TCTGGTATTG TTACAAAAAC AGGATGACAG ACTGGTGTTA 
CGATGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTGTTTGTC 
ATCTCCATGT CGGACAATGG GGCCCAAATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT TATCTGCCGG ATGGCCGCAT CAGTGAAGGA ACAGTCGGCG 
AAGCCAATCC CACTGCCCCA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGAACCT 
CCAAAATTAA AAGTGGAGCA TCATAGCATT TCAGTCCCTG GTCTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAGTCTCC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGGGTGCC 
TCTGGGAAGT CCGAGGTAGA TGATCTGTTT GTGGCTGAGA GACAGTTTGC TAAGGAGCGG 
CATGCAGATG GGACTCTGCA GGAAGCTGGA GCAAGTTACC AAATTCCAAT CCCAGACCCG 
CACTTGCCTG CCTCAGAAGA ACGGTGGGCC TTGGACGCAC TAAGGAATTT GGGCTTGTTG 
AAGCAGTTGC TTGTACAACA GCTGGGTTTG GCTGAGAAGA GCACTCAGGA AGACTGGTCA 
CGTTTCCTGA GATACGGAAC AGCGTCTTCC GGGGTGCAGA CAGACAGTGT AACCCAGAAC 
TCTGAAAATA GTAAGGCCTG TCCTCCTGGT GAAGGACAGG TGTCCTTTAG AACTGAAAGT 
GGTGACATTT CCACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTCTGC TCCACGAGAT 
TCAGTGGTAC TGGCATTCCA AGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG 
CACTTTTTTG ATGCCCGGGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGTG 
GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT CCTTGACGGC 
TACGATGCGG TGCAGGAGAG CTCCACGGAT GAGGAGGTTG CTTCCTCACT CCCCCCACCG 
CCCACGACAG GCGCCCCCCC CACGGACTCT GCCGGCCAGC AGCAGCATTC TCCCCAGAAC 
GCCCATTCCG ATGGGGCGCT CTCACCGTTC ACCCCGGAAT TCCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCC CCTCTTGTGC AGACTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACAACATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006936793.1
CDS	712..5286
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSASHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_006936731.4
Organism	Felis catus(domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006936731.4

ORF Insert Sequence:

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGGTCTTGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGT 
ACATTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGATTCT 
GAAGTAGAGT CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT 
GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAAGAACGG 
CTGCAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACGTCCTCC AGGTCTTTAG GTGACCCCCC GACAGTCAGT 
GAGGTAGAAG CAGACCCTGG TGACGGACTA GGGAGAATTG ACTACAGCAG CGGAGATGCT 
TCTCGGCCCA GTAGTGACAG CGCGGAAAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGCGAAGTG ATTCAGGACA CTGACACTCA GTCACTCATC 
GGGAGCCCCT CTCCCCGAAT GGCACCTCAT ATTATTGGAG CAGAGGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTAAAA 
TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCACGTAG TTTCACAGTT TGACCCTGCA 
ACGTTGCTCT GTTATCTCTA TTCAGACCTA TATAAGCAGA CCAATTCCAA GGAGACTCGG 
CGTGTCTTCC TCGAGTTTCA TCAGTTTTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATATCAAT AGATCTAGAA AAAAGAAGAC CTGAGCTTAT ACCTGAGGAT 
CTACACCGCC ACTATATTCA AACTATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC 
TTGGAAGATT TTCGGCAGAA ACGTAGCATG GGGCTGACCT TGGCTGAAAG CGAGCTGACA 
AAACTCGATG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCG 
GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAT 
AAAAGTTCAA CAATGCAGTA TGTTATCCTT ATGTACATGA AGCATTTGGG AGTAAAAGTG 
AAAGAACCTC GAAATTTGGA GCACAAGCGA GGTCGGATTG GATTCCTTCC AAAAATCAAG 
CAAAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGGA AGCGCAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCTATG 
GAACTACAGA AGCAGCGGCA CCCTAAGCAC TTATCCACAC CCTCTTCTGT GAGTCCTGAG 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAG GTGAAGGAAC AGACGTTGGG 
TACCTGCCAG CCAATTCCAT GTCCTCTGGG GCTTCAGGGG CCACTCTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATCTGGG ATCAAAGCAA GTTGGAGAAA CACCTGCATC CGGAGACTGC 
ATGGATGGCG CCCCTCGCAC TCCCAATACC ATCTTCGATT TCCCACCTCC TCCGTTAGAC 
CAAGTGCAGG AGGAAGAGTG GGAGGTGGAA AGGGTGCCTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAAT TCGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG 
GGACTAAAGC CTTGTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTACCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCTTC AGAACTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCAGTTC GCAAGAGGAA TGAGACCTCA 
GTTATTGATC AGATTGGGGA AGATTTACTG ACCTGGTTCA GCGGACCAGG GGAGGAGAAG 
TTGAAACACG CTGCTGCCAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC CCGATTTCAG ACTTTTGTGC AAGATGCCGA GAGCAATCCG 
CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AAATGCAGAG GCTTACTAAG 
TACCCTCTTC TGTTAGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAACT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ATAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTCGATAC CTCCAATCTG 
AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCACT GGTCTGGAAG GTGAATCGGG ATAAAACTAT CGATCTGTAC 
ACGTTGCTGC TGGAAGACAT TCTGGTATTG TTACAAAAAC AGGATGACAG ACTGGTGTTA 
CGATGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTGTTTGTC 
ATCTCCATGT CGGACAATGG GGCCCAAATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT TATCTGCCGG ATGGCCGCAT CAGTGAAGGA ACAGTCGGCG 
AAGCCAATCC CACTGCCCCA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGAACCT 
CCAAAATTAA AAGTGGAGCA TCATAGCATT TCAGTCCCTG GTCTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAGTCTCC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGGGTGCC 
TCTGGGAAGT CCGAGGTAGA TGATCTGTTT GTGGCTGAGA GACAGTTTGC TAAGGAGCGG 
CATGCAGATG GGACTCTGCA GGAAGCTGGA GCAAGTTACC AAATTCCAAT CCCAGACCCG 
CACTTGCCTG CCTCAGAAGA ACGGTGGGCC TTGGACGCAC TAAGGAATTT GGGCTTGTTG 
AAGCAGTTGC TTGTACAACA GCTGGGTTTG GCTGAGAAGA GCACTCAGGA AGACTGGTCA 
CGTTTCCTGA GATACGGAAC AGCGTCTTCC GGGGTGCAGA CAGACAGTGT AACCCAGAAC 
TCTGAAAATA GTAAGGCCTG TCCTCCTGGT GAAGGACAGG TGTCCTTTAG AACTGAAAGT 
GGTGACATTT CCACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTCTGC TCCACGAGAT 
TCAGTGGTAC TGGCATTCCA AGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG 
CACTTTTTTG ATGCCCGGGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGTG 
GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT CCTTGACGGC 
TACGATGCGG TGCAGGAGAG CTCCACGGAT GAGGAGGTTG CTTCCTCACT CCCCCCACCG 
CCCACGACAG GCGCCCCCCC CACGGACTCT GCCGGCCAGC AGCAGCATTC TCCCCAGAAC 
GCCCATTCCG ATGGGGCGCT CTCACCGTTC ACCCCGGAAT TCCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCC CCTCTTGTGC AGACTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACAACATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_006936731.3 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_006936793.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb14135
Clone ID Related Accession (Same CDS sequence)	XM_006936731.4 , XM_006936731.3
Accession Version	XM_006936731.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006936731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGGTCTTGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGT 
ACATTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGATTCT 
GAAGTAGAGT CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT 
GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAAGAACGG 
CTGCAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACGTCCTCC AGGTCTTTAG GTGACCCCCC GACAGTCAGT 
GAGGTAGAAG CAGACCCTGG TGACGGACTA GGGAGAATTG ACTACAGCAG CGGAGATGCT 
TCTCGGCCCA GTAGTGACAG CGCGGAAAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGCGAAGTG ATTCAGGACA CTGACACTCA GTCACTCATC 
GGGAGCCCCT CTCCCCGAAT GGCACCTCAT ATTATTGGAG CAGAGGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTAAAA 
TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCACGTAG TTTCACAGTT TGACCCTGCA 
ACGTTGCTCT GTTATCTCTA TTCAGACCTA TATAAGCAGA CCAATTCCAA GGAGACTCGG 
CGTGTCTTCC TCGAGTTTCA TCAGTTTTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATATCAAT AGATCTAGAA AAAAGAAGAC CTGAGCTTAT ACCTGAGGAT 
CTACACCGCC ACTATATTCA AACTATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC 
TTGGAAGATT TTCGGCAGAA ACGTAGCATG GGGCTGACCT TGGCTGAAAG CGAGCTGACA 
AAACTCGATG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCG 
GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAT 
AAAAGTTCAA CAATGCAGTA TGTTATCCTT ATGTACATGA AGCATTTGGG AGTAAAAGTG 
AAAGAACCTC GAAATTTGGA GCACAAGCGA GGTCGGATTG GATTCCTTCC AAAAATCAAG 
CAAAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGGA AGCGCAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCTATG 
GAACTACAGA AGCAGCGGCA CCCTAAGCAC TTATCCACAC CCTCTTCTGT GAGTCCTGAG 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAG GTGAAGGAAC AGACGTTGGG 
TACCTGCCAG CCAATTCCAT GTCCTCTGGG GCTTCAGGGG CCACTCTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATCTGGG ATCAAAGCAA GTTGGAGAAA CACCTGCATC CGGAGACTGC 
ATGGATGGCG CCCCTCGCAC TCCCAATACC ATCTTCGATT TCCCACCTCC TCCGTTAGAC 
CAAGTGCAGG AGGAAGAGTG GGAGGTGGAA AGGGTGCCTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAAT TCGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG 
GGACTAAAGC CTTGTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTACCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCTTC AGAACTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCAGTTC GCAAGAGGAA TGAGACCTCA 
GTTATTGATC AGATTGGGGA AGATTTACTG ACCTGGTTCA GCGGACCAGG GGAGGAGAAG 
TTGAAACACG CTGCTGCCAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC CCGATTTCAG ACTTTTGTGC AAGATGCCGA GAGCAATCCG 
CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AAATGCAGAG GCTTACTAAG 
TACCCTCTTC TGTTAGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAACT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ATAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTCGATAC CTCCAATCTG 
AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCACT GGTCTGGAAG GTGAATCGGG ATAAAACTAT CGATCTGTAC 
ACGTTGCTGC TGGAAGACAT TCTGGTATTG TTACAAAAAC AGGATGACAG ACTGGTGTTA 
CGATGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTGTTTGTC 
ATCTCCATGT CGGACAATGG GGCCCAAATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT TATCTGCCGG ATGGCCGCAT CAGTGAAGGA ACAGTCGGCG 
AAGCCAATCC CACTGCCCCA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGAACCT 
CCAAAATTAA AAGTGGAGCA TCATAGCATT TCAGTCCCTG GTCTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAGTCTCC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGGGTGCC 
TCTGGGAAGT CCGAGGTAGA TGATCTGTTT GTGGCTGAGA GACAGTTTGC TAAGGAGCGG 
CATGCAGATG GGACTCTGCA GGAAGCTGGA GCAAGTTACC AAATTCCAAT CCCAGACCCG 
CACTTGCCTG CCTCAGAAGA ACGGTGGGCC TTGGACGCAC TAAGGAATTT GGGCTTGTTG 
AAGCAGTTGC TTGTACAACA GCTGGGTTTG GCTGAGAAGA GCACTCAGGA AGACTGGTCA 
CGTTTCCTGA GATACGGAAC AGCGTCTTCC GGGGTGCAGA CAGACAGTGT AACCCAGAAC 
TCTGAAAATA GTAAGGCCTG TCCTCCTGGT GAAGGACAGG TGTCCTTTAG AACTGAAAGT 
GGTGACATTT CCACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTCTGC TCCACGAGAT 
TCAGTGGTAC TGGCATTCCA AGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG 
CACTTTTTTG ATGCCCGGGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGTG 
GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT CCTTGACGGC 
TACGATGCGG TGCAGGAGAG CTCCACGGAT GAGGAGGTTG CTTCCTCACT CCCCCCACCG 
CCCACGACAG GCGCCCCCCC CACGGACTCT GCCGGCCAGC AGCAGCATTC TCCCCAGAAC 
GCCCATTCCG ATGGGGCGCT CTCACCGTTC ACCCCGGAAT TCCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCC CCTCTTGTGC AGACTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACAACATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006936793.1
CDS	713..5287
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSASHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_006936731.3
Organism	Felis catus(Domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006936731.3

ORF Insert Sequence:

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGGTCTTGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGT 
ACATTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGATTCT 
GAAGTAGAGT CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT 
GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAAGAACGG 
CTGCAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACGTCCTCC AGGTCTTTAG GTGACCCCCC GACAGTCAGT 
GAGGTAGAAG CAGACCCTGG TGACGGACTA GGGAGAATTG ACTACAGCAG CGGAGATGCT 
TCTCGGCCCA GTAGTGACAG CGCGGAAAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGCGAAGTG ATTCAGGACA CTGACACTCA GTCACTCATC 
GGGAGCCCCT CTCCCCGAAT GGCACCTCAT ATTATTGGAG CAGAGGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTAAAA 
TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCACGTAG TTTCACAGTT TGACCCTGCA 
ACGTTGCTCT GTTATCTCTA TTCAGACCTA TATAAGCAGA CCAATTCCAA GGAGACTCGG 
CGTGTCTTCC TCGAGTTTCA TCAGTTTTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATATCAAT AGATCTAGAA AAAAGAAGAC CTGAGCTTAT ACCTGAGGAT 
CTACACCGCC ACTATATTCA AACTATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC 
TTGGAAGATT TTCGGCAGAA ACGTAGCATG GGGCTGACCT TGGCTGAAAG CGAGCTGACA 
AAACTCGATG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCG 
GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAT 
AAAAGTTCAA CAATGCAGTA TGTTATCCTT ATGTACATGA AGCATTTGGG AGTAAAAGTG 
AAAGAACCTC GAAATTTGGA GCACAAGCGA GGTCGGATTG GATTCCTTCC AAAAATCAAG 
CAAAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGGA AGCGCAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCTATG 
GAACTACAGA AGCAGCGGCA CCCTAAGCAC TTATCCACAC CCTCTTCTGT GAGTCCTGAG 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAG GTGAAGGAAC AGACGTTGGG 
TACCTGCCAG CCAATTCCAT GTCCTCTGGG GCTTCAGGGG CCACTCTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATCTGGG ATCAAAGCAA GTTGGAGAAA CACCTGCATC CGGAGACTGC 
ATGGATGGCG CCCCTCGCAC TCCCAATACC ATCTTCGATT TCCCACCTCC TCCGTTAGAC 
CAAGTGCAGG AGGAAGAGTG GGAGGTGGAA AGGGTGCCTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAAT TCGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG 
GGACTAAAGC CTTGTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTACCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCTTC AGAACTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCAGTTC GCAAGAGGAA TGAGACCTCA 
GTTATTGATC AGATTGGGGA AGATTTACTG ACCTGGTTCA GCGGACCAGG GGAGGAGAAG 
TTGAAACACG CTGCTGCCAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC CCGATTTCAG ACTTTTGTGC AAGATGCCGA GAGCAATCCG 
CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AAATGCAGAG GCTTACTAAG 
TACCCTCTTC TGTTAGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAACT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ATAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTCGATAC CTCCAATCTG 
AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCACT GGTCTGGAAG GTGAATCGGG ATAAAACTAT CGATCTGTAC 
ACGTTGCTGC TGGAAGACAT TCTGGTATTG TTACAAAAAC AGGATGACAG ACTGGTGTTA 
CGATGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTGTTTGTC 
ATCTCCATGT CGGACAATGG GGCCCAAATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT TATCTGCCGG ATGGCCGCAT CAGTGAAGGA ACAGTCGGCG 
AAGCCAATCC CACTGCCCCA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGAACCT 
CCAAAATTAA AAGTGGAGCA TCATAGCATT TCAGTCCCTG GTCTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAGTCTCC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGGGTGCC 
TCTGGGAAGT CCGAGGTAGA TGATCTGTTT GTGGCTGAGA GACAGTTTGC TAAGGAGCGG 
CATGCAGATG GGACTCTGCA GGAAGCTGGA GCAAGTTACC AAATTCCAAT CCCAGACCCG 
CACTTGCCTG CCTCAGAAGA ACGGTGGGCC TTGGACGCAC TAAGGAATTT GGGCTTGTTG 
AAGCAGTTGC TTGTACAACA GCTGGGTTTG GCTGAGAAGA GCACTCAGGA AGACTGGTCA 
CGTTTCCTGA GATACGGAAC AGCGTCTTCC GGGGTGCAGA CAGACAGTGT AACCCAGAAC 
TCTGAAAATA GTAAGGCCTG TCCTCCTGGT GAAGGACAGG TGTCCTTTAG AACTGAAAGT 
GGTGACATTT CCACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTCTGC TCCACGAGAT 
TCAGTGGTAC TGGCATTCCA AGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG 
CACTTTTTTG ATGCCCGGGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGTG 
GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT CCTTGACGGC 
TACGATGCGG TGCAGGAGAG CTCCACGGAT GAGGAGGTTG CTTCCTCACT CCCCCCACCG 
CCCACGACAG GCGCCCCCCC CACGGACTCT GCCGGCCAGC AGCAGCATTC TCCCCAGAAC 
GCCCATTCCG ATGGGGCGCT CTCACCGTTC ACCCCGGAAT TCCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCC CCTCTTGTGC AGACTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACAACATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_019811430.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666989.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

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CloneID	OFb49425
Clone ID Related Accession (Same CDS sequence)	XM_019811430.2 , XM_019811430.1
Accession Version	XM_019811430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019811430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666989.1
CDS	714..5225
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_019811430.2
Organism	Felis catus(domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019811430.2

ORF Insert Sequence:

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_019811430.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666989.1 Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

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CloneID	OFb49425
Clone ID Related Accession (Same CDS sequence)	XM_019811430.2 , XM_019811430.1
Accession Version	XM_019811430.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018732.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666989.1
CDS	713..5224
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVESSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQDGAVVTSSRSLGDPPTVSEVEADPGDGLGRIDYSSGDASRPSSDSAESPKSALKERIYLEENPEKSEVIQDTDTQSLIGSPSPRMAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISIDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRVTLEKERACAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGLAGEGTDVGYLPANSMSSGASGATLSQEGGKENDLGSKQVGETPASGDCMDGAPRTPNTIFDFPPPPLDQVQEEEWEVERVPEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPMEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSAKPIPLPQSPPCEGDNDEEEPPKLKVEHHSISVPGLQSPDRDLGLESPLISSKPQSHSLGASGKSEVDDLFVAERQFAKERHADGTLQEAGASYQIPIPDPHLPASEERWALDALRNLGLLKQLLVQQLGLAEKSTQEDWSRFLRYGTASSGVQTDSVTQNSENSKACPPGEGQVSFRTESGDISTCYSPRTSTESSAPRDSVVLAFQDSQASNILVMDHMIMTPEMPPAEPEGSLDESGEHFFDAREAHSDDNPSEGDGVVKKEEDVNLRISGNYLILDGYDAVQESSTDEEVASSLPPPPTTGAPPTDSAGQQQHSPQNAHSDGALSPFTPEFLVQQRWGAMEDSCFEIQSPPSCADSQSQIMEYIRKIEADLEHLKKVEESYNILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_019811430.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019811430.1

ORF Insert Sequence:

ATGAGCGGCA CACAGTCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA 
TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGTACA 
TTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGATTCTGAA 
GTAGAGTCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA 
GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA AGAACGGCTG 
CAGGATGGAG CTGTAGTTAC GTCCTCCAGG TCTTTAGGTG ACCCCCCGAC AGTCAGTGAG 
GTAGAAGCAG ACCCTGGTGA CGGACTAGGG AGAATTGACT ACAGCAGCGG AGATGCTTCT 
CGGCCCAGTA GTGACAGCGC GGAAAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAGAAAAG CGAAGTGATT CAGGACACTG ACACTCAGTC ACTCATCGGG 
AGCCCCTCTC CCCGAATGGC ACCTCATATT ATTGGAGCAG AGGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CACGTAGTTT CACAGTTTGA CCCTGCAACG 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAGCAGACCA ATTCCAAGGA GACTCGGCGT 
GTCTTCCTCG AGTTTCATCA GTTTTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TATCAATAGA TCTAGAAAAA AGAAGACCTG AGCTTATACC TGAGGATCTA 
CACCGCCACT ATATTCAAAC TATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAACG TAGCATGGGG CTGACCTTGG CTGAAAGCGA GCTGACAAAA 
CTCGATGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCGGAA 
CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGATAAA 
AGTTCAACAA TGCAGTATGT TATCCTTATG TACATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGGAAGC GCAGAGGATT CCCCAGCATC 
CTGGGACCCC CAAGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCTATGGAA 
CTACAGAAGC AGCGGCACCC TAAGCACTTA TCCACACCCT CTTCTGTGAG TCCTGAGCCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAGGTG AAGGAACAGA CGTTGGGTAC 
CTGCCAGCCA ATTCCATGTC CTCTGGGGCT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATCTGGGATC AAAGCAAGTT GGAGAAACAC CTGCATCCGG AGACTGCATG 
GATGGCGCCC CTCGCACTCC CAATACCATC TTCGATTTCC CACCTCCTCC GTTAGACCAA 
GTGCAGGAGG AAGAGTGGGA GGTGGAAAGG GTGCCTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAATTCG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ACCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCAGTTCGCA AGAGGAATGA GACCTCAGTT 
ATTGATCAGA TTGGGGAAGA TTTACTGACC TGGTTCAGCG GACCAGGGGA GGAGAAGTTG 
AAACACGCTG CTGCCACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCCCG ATTTCAGACT TTTGTGCAAG ATGCCGAGAG CAATCCGCTG 
TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTGT TAGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAACTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAATA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TCGATACCTC CAATCTGAAG 
TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCACTGGT CTGGAAGGTG AATCGGGATA AAACTATCGA TCTGTACACG 
TTGCTGCTGG AAGACATTCT GGTATTGTTA CAAAAACAGG ATGACAGACT GGTGTTACGA 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT GTTTGTCATC 
TCCATGTCGG ACAATGGGGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTTAT CTGCCGGATG GCCGCATCAG TGAAGGAACA GTCGGCGAAG 
CCAATCCCAC TGCCCCAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAGCATCA TAGCATTTCA GTCCCTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCCTT AATATCGTCA AAACCTCAGT CTCATTCACT GGGTGCCTCT 
GGGAAGTCCG AGGTAGATGA TCTGTTTGTG GCTGAGAGAC AGTTTGCTAA GGAGCGGCAT 
GCAGATGGGA CTCTGCAGGA AGCTGGAGCA AGTTACCAAA TTCCAATCCC AGACCCGCAC 
TTGCCTGCCT CAGAAGAACG GTGGGCCTTG GACGCACTAA GGAATTTGGG CTTGTTGAAG 
CAGTTGCTTG TACAACAGCT GGGTTTGGCT GAGAAGAGCA CTCAGGAAGA CTGGTCACGT 
TTCCTGAGAT ACGGAACAGC GTCTTCCGGG GTGCAGACAG ACAGTGTAAC CCAGAACTCT 
GAAAATAGTA AGGCCTGTCC TCCTGGTGAA GGACAGGTGT CCTTTAGAAC TGAAAGTGGT 
GACATTTCCA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGAGATTCA 
GTGGTACTGG CATTCCAAGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGGGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGTGGTT 
AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATCCT TGACGGCTAC 
GATGCGGTGC AGGAGAGCTC CACGGATGAG GAGGTTGCTT CCTCACTCCC CCCACCGCCC 
ACGACAGGCG CCCCCCCCAC GGACTCTGCC GGCCAGCAGC AGCATTCTCC CCAGAACGCC 
CATTCCGATG GGGCGCTCTC ACCGTTCACC CCGGAATTCC TGGTCCAGCA GCGCTGGGGA 
GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGTCCCCCCT CTTGTGCAGA CTCACAAAGC 
CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTTG AACACTTAAA GAAGGTGGAG 
GAAAGTTACA ACATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA 
GATAAAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ARHGEF12 cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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