Lgr1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lgr1
Entrez Gene ID	42133
Full Name	Leucine-rich repeat-containing G protein-coupled receptor 1
Synonyms	CG7665,DLGR-1,DLGR1,Dmel\CG7665,FSH,FSH-TSH,Fsh,Fsh/CG7665,LGR1,LH/CG receptor,RH44949p,TSH,dLGR1
General protein information	Preferred Names Leucine-rich repeat-containing G protein-coupled receptor 1 Names CG7665 gene product from transcript CG7665-RB CG7665-PA CG7665-PB Fsh-Tsh-like receptor Leu-rich repeats-containing G protein-coupled receptor Lgr1-PA Lgr1-PB glycoprotein A2/Glycoprotein B5 receptor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90C2-90C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079669.3	NP_524393.2	Leucine-rich repeat-containing G protein-coupled receptor 1, isoform A
NM_169777.2	NP_732265.1	Leucine-rich repeat-containing G protein-coupled receptor 1, isoform B

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LHCGR	XP_001114090.1
Bos taurus (cattle)	LHCGR	NP_776806.1
Drosophila melanogaster (fruit fly)	Lgr1	NP_524393.2
Homo sapiens (human)	LHCGR	NP_000224.2
Rattus norvegicus (Norway rat)	Lhcgr	NP_037110.1
Xenopus tropicalis (tropical clawed frog)	lhcgr	XP_002935423.2
Anopheles gambiae (African malaria mosquito)	GPRFSH	XP_309589.2
Canis lupus familiaris (dog)	LHCGR	XP_538486.2
Gallus gallus (chicken)	LHCGR	NP_990267.1
Danio rerio (zebrafish)	lhcgr	NP_991188.1
Caenorhabditis elegans (roundworm)	fshr-1	NP_505548.1
Mus musculus (house mouse)	Lhcgr	NP_038610.1
Pan troglodytes (chimpanzee)	LHCGR	XP_003309053.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lgr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lgr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05262	NM_079669.3 Latest version!	Drosophila melanogaster Leucine-rich repeat-containing G protein-coupled receptor 1 (Lgr1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa05262	NM_169777.2 Latest version!	Drosophila melanogaster Leucine-rich repeat-containing G protein-coupled receptor 1 (Lgr1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lgr1 ( NM_079669.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524393.2 Drosophila melanogaster Leucine-rich repeat-containing G protein-coupled receptor 1 (Lgr1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05262
Clone ID Related Accession (Same CDS sequence)	NM_079669.3 , NM_169777.2
Accession Version	NM_079669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich repeat-containing G protein-coupled receptor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079669.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC ACCCGAGTCT GTCCCAGCGG ATGGGTACCA CTTACCGTCC GAGGAAGGGC 
CTGAAGTGTC TGTCATTCGA GTTCCAGTGC CGCCTGCTTC TCCACCATCT GCTGCTAACG 
TCGCTGAGTG GCCGGCATTT CGTATACGCC ACTAGTGCCG TCGGGGGGGC GCTGAGTGCC 
AACAACTGTC ACGACATCCA CCACGGATTC GATGTCTACC CCAATCTCAC GGCCGTATCG 
CTGGCTCAAT CGACGGACAC GCCCCTGACC GCGACGATGC CACGGTCCGC CTGGAAGTGT 
TGTTGCTGGA ATGCAAGTAA TCAAGCGGAG GAGGTGGAGT GCCGCTGCGA GGGCGATGGA 
CTGAATCGCG TACCGCAAAC CCTGACGCTG CCCATACAGC GTCTAACTAT CGCATCGGCG 
GGGCTGCCAC GCCTACGCCA CACGGGGCTG AAGGTCTACG GATCCACGTT GCTGGATGTG 
GCCTTCACCG ACTGCCTGCA GCTGGAACTG ATACAGGATG GCGCTTTTGC TAATTTAACC 
CTATTGCGAA CAATCTACAT CACCAACGCA CCCAAGCTGA CTTTCCTCTC GAAGGACGTA 
TTCCTTGGCA TTTCGGACAC CGTGGACATT ATACGAATCA TAAATAGTGG CCTGACCAGA 
GTTCCTGACT TGGGCCACCT GCCACCTCAT AATATTCTGC AAATGATAGA TCTCGATAAC 
AATCAAATCA CTCGGATAGA CAGTAAATCT ATAAAAGTAA AGACTGCCCA ATTAATTTTG 
ACAAACAATG AAATAAGCTA CGTTGATGAC TCCGCTTTCT TCGGCTCCAA GATAGCCAAA 
CTATCGCTGA AGGAAAACAA AAAACTGCAA ATGATGCATC CCAACGCATT CGATGGCATC 
ATAGACATAA CTGAACTCGA TCTCTCCAGC ACCTCGTTAG TTGGCCTTCC TTCTGCCGGA 
CTTCAGAACA TCGAAGCGTT GTACATTCAG AACACCCACA CTTTAAAGAC CATACCATCC 
ATCTACAACT TCCGGAATCT GCAGAGGGCC TATCTCACCC ACTCCTTCCA CTGCTGCGCC 
TTCCAGTTTC CATCTCGGCA CGATCCTCAG CGACACGCCC AGCGGATGCT GGAGATTGAG 
AAATGGCGGA AGCAGTGCAA AAGTGACTCC GGAACGCGAA AGGAGAGATC AACCTTGGAT 
AACCCCTTCA ATATGCCAGA AGATTTTGGT TCTTTCGGTG GCACCGACGA CTCAGCAACA 
GATATAACCC CCATCACTTT CGCTTCCTTC GATTACATGG CCGATGACAC CATGAACAAG 
GGCACTTTTC ACGAAAAGAT CATCCTGAAT CCGGGTGATG ACTCCTCGGC CGAACTTTGC 
GGCAATTTTA CTTTCAGAAA ACCCAATATT GAATGCTATC CCATGCCCAA TGATCTAAAT 
CCTTGCGAGG ATGTAATGGG CTATCAATGG CTGCGTATTT CCGTCTGGAT TGTGGTGGCT 
CTGGCCGTCG TGGGCAACGT GGCTGTGCTG ACAGTAATAC TATCGATTAG GCCGGAGTCG 
ACGCCGGTGC CGCGATTTCT GATGTGCCAC CTGGCCTTCG CCGACCTGTG CTTGGGCCTC 
TATCTGCTGC TGGTGGCCTG CATCGATGCG CATTCCATGG GGGAGTATTT CAATTTCGCC 
TACGATTGGC AGTACGGGCT TGGCTGCAAA GTAGCCGGCT TTCTCACCGT GTTCGCCAGT 
CATTTGTCGG TGTTCACGCT GACCGTGATT ACCATCGAGC GCTGGCTGGC AATCACGCAG 
GCCATGTACC TGAACCATCG CATCAAGTTG CGTCCTGCAG CGCTCATCAT GCTGGGCGGC 
TGGATTTACT CGATGCTTAT GTCCTCGCTG CCCCTGTTTG GCATAAGTAA TTATTCGTCG 
ACGAGCATTT GCCTTCCGAT GGAAAATCGC GATGTGTACG ACACCATTTA CCTGATTGCC 
ATTCTGGGCT CCAACGGAGT GGCCTTCTCC ATCATAGCCG TCTGCTATGC CCAGATCTAT 
TTGTCCTTGG GTCGCGAGAC ACGGCAGGCC CACCAGAATA GCCCGGGCGA GTTGAGCGTT 
GCCAAGAAGA TGGCCCTTTT GGTCTTTACA AACTTTGCGT GCTGGTCGCC TATTGCTTTC 
TTTGGACTCA CCGCGTTGGC CGGATACCCA CTGATTAATG TTACCAAGTC GAAGATATTG 
CTAGTCTTCT TCTATCCCTT GAATTCCTGC GCGGATCCCT ATCTGTATGC CATATTGACG 
TCCCAGTATC GCCAGGATCT GTTCACCTTG TTGTCCAAAT TGGGCCTGTG CCAGCAGAGC 
GCTCTGAAGT ACAAGGATAG CCTTTCCGGC CAAGCCACCA CCAGATTTAC AATCCACGGC 
TCCATTCAGA GGCACAGCTC ACTCACGTGC AAAATGCAGA CTGTGATGGG CGCTGAGACG 
CAGAAAATGC TGAAGAACAG CGAGGATTAT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524393.2
CDS	266..2761
Translation	MEKHPSLSQRMGTTYRPRKGLKCLSFEFQCRLLLHHLLLTSLSGRHFVYATSAVGGALSANNCHDIHHGFDVYPNLTAVSLAQSTDTPLTATMPRSAWKCCCWNASNQAEEVECRCEGDGLNRVPQTLTLPIQRLTIASAGLPRLRHTGLKVYGSTLLDVAFTDCLQLELIQDGAFANLTLLRTIYITNAPKLTFLSKDVFLGISDTVDIIRIINSGLTRVPDLGHLPPHNILQMIDLDNNQITRIDSKSIKVKTAQLILTNNEISYVDDSAFFGSKIAKLSLKENKKLQMMHPNAFDGIIDITELDLSSTSLVGLPSAGLQNIEALYIQNTHTLKTIPSIYNFRNLQRAYLTHSFHCCAFQFPSRHDPQRHAQRMLEIEKWRKQCKSDSGTRKERSTLDNPFNMPEDFGSFGGTDDSATDITPITFASFDYMADDTMNKGTFHEKIILNPGDDSSAELCGNFTFRKPNIECYPMPNDLNPCEDVMGYQWLRISVWIVVALAVVGNVAVLTVILSIRPESTPVPRFLMCHLAFADLCLGLYLLLVACIDAHSMGEYFNFAYDWQYGLGCKVAGFLTVFASHLSVFTLTVITIERWLAITQAMYLNHRIKLRPAALIMLGGWIYSMLMSSLPLFGISNYSSTSICLPMENRDVYDTIYLIAILGSNGVAFSIIAVCYAQIYLSLGRETRQAHQNSPGELSVAKKMALLVFTNFACWSPIAFFGLTALAGYPLINVTKSKILLVFFYPLNSCADPYLYAILTSQYRQDLFTLLSKLGLCQQSALKYKDSLSGQATTRFTIHGSIQRHSSLTCKMQTVMGAETQKMLKNSEDYV

Target ORF information:

RefSeq Version	NM_079669.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich repeat-containing G protein-coupled receptor 1 (Lgr1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079669.3

ORF Insert Sequence:

ATGGAAAAGC ACCCGAGTCT GTCCCAGCGG ATGGGTACCA CTTACCGTCC GAGGAAGGGC 
CTGAAGTGTC TGTCATTCGA GTTCCAGTGC CGCCTGCTTC TCCACCATCT GCTGCTAACG 
TCGCTGAGTG GCCGGCATTT CGTATACGCC ACTAGTGCCG TCGGGGGGGC GCTGAGTGCC 
AACAACTGTC ACGACATCCA CCACGGATTC GATGTCTACC CCAATCTCAC GGCCGTATCG 
CTGGCTCAAT CGACGGACAC GCCCCTGACC GCGACGATGC CACGGTCCGC CTGGAAGTGT 
TGTTGCTGGA ATGCAAGTAA TCAAGCGGAG GAGGTGGAGT GCCGCTGCGA GGGCGATGGA 
CTGAATCGCG TACCGCAAAC CCTGACGCTG CCCATACAGC GTCTAACTAT CGCATCGGCG 
GGGCTGCCAC GCCTACGCCA CACGGGGCTG AAGGTCTACG GATCCACGTT GCTGGATGTG 
GCCTTCACCG ACTGCCTGCA GCTGGAACTG ATACAGGATG GCGCTTTTGC TAATTTAACC 
CTATTGCGAA CAATCTACAT CACCAACGCA CCCAAGCTGA CTTTCCTCTC GAAGGACGTA 
TTCCTTGGCA TTTCGGACAC CGTGGACATT ATACGAATCA TAAATAGTGG CCTGACCAGA 
GTTCCTGACT TGGGCCACCT GCCACCTCAT AATATTCTGC AAATGATAGA TCTCGATAAC 
AATCAAATCA CTCGGATAGA CAGTAAATCT ATAAAAGTAA AGACTGCCCA ATTAATTTTG 
ACAAACAATG AAATAAGCTA CGTTGATGAC TCCGCTTTCT TCGGCTCCAA GATAGCCAAA 
CTATCGCTGA AGGAAAACAA AAAACTGCAA ATGATGCATC CCAACGCATT CGATGGCATC 
ATAGACATAA CTGAACTCGA TCTCTCCAGC ACCTCGTTAG TTGGCCTTCC TTCTGCCGGA 
CTTCAGAACA TCGAAGCGTT GTACATTCAG AACACCCACA CTTTAAAGAC CATACCATCC 
ATCTACAACT TCCGGAATCT GCAGAGGGCC TATCTCACCC ACTCCTTCCA CTGCTGCGCC 
TTCCAGTTTC CATCTCGGCA CGATCCTCAG CGACACGCCC AGCGGATGCT GGAGATTGAG 
AAATGGCGGA AGCAGTGCAA AAGTGACTCC GGAACGCGAA AGGAGAGATC AACCTTGGAT 
AACCCCTTCA ATATGCCAGA AGATTTTGGT TCTTTCGGTG GCACCGACGA CTCAGCAACA 
GATATAACCC CCATCACTTT CGCTTCCTTC GATTACATGG CCGATGACAC CATGAACAAG 
GGCACTTTTC ACGAAAAGAT CATCCTGAAT CCGGGTGATG ACTCCTCGGC CGAACTTTGC 
GGCAATTTTA CTTTCAGAAA ACCCAATATT GAATGCTATC CCATGCCCAA TGATCTAAAT 
CCTTGCGAGG ATGTAATGGG CTATCAATGG CTGCGTATTT CCGTCTGGAT TGTGGTGGCT 
CTGGCCGTCG TGGGCAACGT GGCTGTGCTG ACAGTAATAC TATCGATTAG GCCGGAGTCG 
ACGCCGGTGC CGCGATTTCT GATGTGCCAC CTGGCCTTCG CCGACCTGTG CTTGGGCCTC 
TATCTGCTGC TGGTGGCCTG CATCGATGCG CATTCCATGG GGGAGTATTT CAATTTCGCC 
TACGATTGGC AGTACGGGCT TGGCTGCAAA GTAGCCGGCT TTCTCACCGT GTTCGCCAGT 
CATTTGTCGG TGTTCACGCT GACCGTGATT ACCATCGAGC GCTGGCTGGC AATCACGCAG 
GCCATGTACC TGAACCATCG CATCAAGTTG CGTCCTGCAG CGCTCATCAT GCTGGGCGGC 
TGGATTTACT CGATGCTTAT GTCCTCGCTG CCCCTGTTTG GCATAAGTAA TTATTCGTCG 
ACGAGCATTT GCCTTCCGAT GGAAAATCGC GATGTGTACG ACACCATTTA CCTGATTGCC 
ATTCTGGGCT CCAACGGAGT GGCCTTCTCC ATCATAGCCG TCTGCTATGC CCAGATCTAT 
TTGTCCTTGG GTCGCGAGAC ACGGCAGGCC CACCAGAATA GCCCGGGCGA GTTGAGCGTT 
GCCAAGAAGA TGGCCCTTTT GGTCTTTACA AACTTTGCGT GCTGGTCGCC TATTGCTTTC 
TTTGGACTCA CCGCGTTGGC CGGATACCCA CTGATTAATG TTACCAAGTC GAAGATATTG 
CTAGTCTTCT TCTATCCCTT GAATTCCTGC GCGGATCCCT ATCTGTATGC CATATTGACG 
TCCCAGTATC GCCAGGATCT GTTCACCTTG TTGTCCAAAT TGGGCCTGTG CCAGCAGAGC 
GCTCTGAAGT ACAAGGATAG CCTTTCCGGC CAAGCCACCA CCAGATTTAC AATCCACGGC 
TCCATTCAGA GGCACAGCTC ACTCACGTGC AAAATGCAGA CTGTGATGGG CGCTGAGACG 
CAGAAAATGC TGAAGAACAG CGAGGATTAT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lgr1 ( NM_169777.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732265.1 Drosophila melanogaster Leucine-rich repeat-containing G protein-coupled receptor 1 (Lgr1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05262
Clone ID Related Accession (Same CDS sequence)	NM_079669.3 , NM_169777.2
Accession Version	NM_169777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich repeat-containing G protein-coupled receptor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169777.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC ACCCGAGTCT GTCCCAGCGG ATGGGTACCA CTTACCGTCC GAGGAAGGGC 
CTGAAGTGTC TGTCATTCGA GTTCCAGTGC CGCCTGCTTC TCCACCATCT GCTGCTAACG 
TCGCTGAGTG GCCGGCATTT CGTATACGCC ACTAGTGCCG TCGGGGGGGC GCTGAGTGCC 
AACAACTGTC ACGACATCCA CCACGGATTC GATGTCTACC CCAATCTCAC GGCCGTATCG 
CTGGCTCAAT CGACGGACAC GCCCCTGACC GCGACGATGC CACGGTCCGC CTGGAAGTGT 
TGTTGCTGGA ATGCAAGTAA TCAAGCGGAG GAGGTGGAGT GCCGCTGCGA GGGCGATGGA 
CTGAATCGCG TACCGCAAAC CCTGACGCTG CCCATACAGC GTCTAACTAT CGCATCGGCG 
GGGCTGCCAC GCCTACGCCA CACGGGGCTG AAGGTCTACG GATCCACGTT GCTGGATGTG 
GCCTTCACCG ACTGCCTGCA GCTGGAACTG ATACAGGATG GCGCTTTTGC TAATTTAACC 
CTATTGCGAA CAATCTACAT CACCAACGCA CCCAAGCTGA CTTTCCTCTC GAAGGACGTA 
TTCCTTGGCA TTTCGGACAC CGTGGACATT ATACGAATCA TAAATAGTGG CCTGACCAGA 
GTTCCTGACT TGGGCCACCT GCCACCTCAT AATATTCTGC AAATGATAGA TCTCGATAAC 
AATCAAATCA CTCGGATAGA CAGTAAATCT ATAAAAGTAA AGACTGCCCA ATTAATTTTG 
ACAAACAATG AAATAAGCTA CGTTGATGAC TCCGCTTTCT TCGGCTCCAA GATAGCCAAA 
CTATCGCTGA AGGAAAACAA AAAACTGCAA ATGATGCATC CCAACGCATT CGATGGCATC 
ATAGACATAA CTGAACTCGA TCTCTCCAGC ACCTCGTTAG TTGGCCTTCC TTCTGCCGGA 
CTTCAGAACA TCGAAGCGTT GTACATTCAG AACACCCACA CTTTAAAGAC CATACCATCC 
ATCTACAACT TCCGGAATCT GCAGAGGGCC TATCTCACCC ACTCCTTCCA CTGCTGCGCC 
TTCCAGTTTC CATCTCGGCA CGATCCTCAG CGACACGCCC AGCGGATGCT GGAGATTGAG 
AAATGGCGGA AGCAGTGCAA AAGTGACTCC GGAACGCGAA AGGAGAGATC AACCTTGGAT 
AACCCCTTCA ATATGCCAGA AGATTTTGGT TCTTTCGGTG GCACCGACGA CTCAGCAACA 
GATATAACCC CCATCACTTT CGCTTCCTTC GATTACATGG CCGATGACAC CATGAACAAG 
GGCACTTTTC ACGAAAAGAT CATCCTGAAT CCGGGTGATG ACTCCTCGGC CGAACTTTGC 
GGCAATTTTA CTTTCAGAAA ACCCAATATT GAATGCTATC CCATGCCCAA TGATCTAAAT 
CCTTGCGAGG ATGTAATGGG CTATCAATGG CTGCGTATTT CCGTCTGGAT TGTGGTGGCT 
CTGGCCGTCG TGGGCAACGT GGCTGTGCTG ACAGTAATAC TATCGATTAG GCCGGAGTCG 
ACGCCGGTGC CGCGATTTCT GATGTGCCAC CTGGCCTTCG CCGACCTGTG CTTGGGCCTC 
TATCTGCTGC TGGTGGCCTG CATCGATGCG CATTCCATGG GGGAGTATTT CAATTTCGCC 
TACGATTGGC AGTACGGGCT TGGCTGCAAA GTAGCCGGCT TTCTCACCGT GTTCGCCAGT 
CATTTGTCGG TGTTCACGCT GACCGTGATT ACCATCGAGC GCTGGCTGGC AATCACGCAG 
GCCATGTACC TGAACCATCG CATCAAGTTG CGTCCTGCAG CGCTCATCAT GCTGGGCGGC 
TGGATTTACT CGATGCTTAT GTCCTCGCTG CCCCTGTTTG GCATAAGTAA TTATTCGTCG 
ACGAGCATTT GCCTTCCGAT GGAAAATCGC GATGTGTACG ACACCATTTA CCTGATTGCC 
ATTCTGGGCT CCAACGGAGT GGCCTTCTCC ATCATAGCCG TCTGCTATGC CCAGATCTAT 
TTGTCCTTGG GTCGCGAGAC ACGGCAGGCC CACCAGAATA GCCCGGGCGA GTTGAGCGTT 
GCCAAGAAGA TGGCCCTTTT GGTCTTTACA AACTTTGCGT GCTGGTCGCC TATTGCTTTC 
TTTGGACTCA CCGCGTTGGC CGGATACCCA CTGATTAATG TTACCAAGTC GAAGATATTG 
CTAGTCTTCT TCTATCCCTT GAATTCCTGC GCGGATCCCT ATCTGTATGC CATATTGACG 
TCCCAGTATC GCCAGGATCT GTTCACCTTG TTGTCCAAAT TGGGCCTGTG CCAGCAGAGC 
GCTCTGAAGT ACAAGGATAG CCTTTCCGGC CAAGCCACCA CCAGATTTAC AATCCACGGC 
TCCATTCAGA GGCACAGCTC ACTCACGTGC AAAATGCAGA CTGTGATGGG CGCTGAGACG 
CAGAAAATGC TGAAGAACAG CGAGGATTAT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732265.1
CDS	336..2831
Translation	MEKHPSLSQRMGTTYRPRKGLKCLSFEFQCRLLLHHLLLTSLSGRHFVYATSAVGGALSANNCHDIHHGFDVYPNLTAVSLAQSTDTPLTATMPRSAWKCCCWNASNQAEEVECRCEGDGLNRVPQTLTLPIQRLTIASAGLPRLRHTGLKVYGSTLLDVAFTDCLQLELIQDGAFANLTLLRTIYITNAPKLTFLSKDVFLGISDTVDIIRIINSGLTRVPDLGHLPPHNILQMIDLDNNQITRIDSKSIKVKTAQLILTNNEISYVDDSAFFGSKIAKLSLKENKKLQMMHPNAFDGIIDITELDLSSTSLVGLPSAGLQNIEALYIQNTHTLKTIPSIYNFRNLQRAYLTHSFHCCAFQFPSRHDPQRHAQRMLEIEKWRKQCKSDSGTRKERSTLDNPFNMPEDFGSFGGTDDSATDITPITFASFDYMADDTMNKGTFHEKIILNPGDDSSAELCGNFTFRKPNIECYPMPNDLNPCEDVMGYQWLRISVWIVVALAVVGNVAVLTVILSIRPESTPVPRFLMCHLAFADLCLGLYLLLVACIDAHSMGEYFNFAYDWQYGLGCKVAGFLTVFASHLSVFTLTVITIERWLAITQAMYLNHRIKLRPAALIMLGGWIYSMLMSSLPLFGISNYSSTSICLPMENRDVYDTIYLIAILGSNGVAFSIIAVCYAQIYLSLGRETRQAHQNSPGELSVAKKMALLVFTNFACWSPIAFFGLTALAGYPLINVTKSKILLVFFYPLNSCADPYLYAILTSQYRQDLFTLLSKLGLCQQSALKYKDSLSGQATTRFTIHGSIQRHSSLTCKMQTVMGAETQKMLKNSEDYV

Target ORF information:

RefSeq Version	NM_169777.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich repeat-containing G protein-coupled receptor 1 (Lgr1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169777.2

ORF Insert Sequence:

ATGGAAAAGC ACCCGAGTCT GTCCCAGCGG ATGGGTACCA CTTACCGTCC GAGGAAGGGC 
CTGAAGTGTC TGTCATTCGA GTTCCAGTGC CGCCTGCTTC TCCACCATCT GCTGCTAACG 
TCGCTGAGTG GCCGGCATTT CGTATACGCC ACTAGTGCCG TCGGGGGGGC GCTGAGTGCC 
AACAACTGTC ACGACATCCA CCACGGATTC GATGTCTACC CCAATCTCAC GGCCGTATCG 
CTGGCTCAAT CGACGGACAC GCCCCTGACC GCGACGATGC CACGGTCCGC CTGGAAGTGT 
TGTTGCTGGA ATGCAAGTAA TCAAGCGGAG GAGGTGGAGT GCCGCTGCGA GGGCGATGGA 
CTGAATCGCG TACCGCAAAC CCTGACGCTG CCCATACAGC GTCTAACTAT CGCATCGGCG 
GGGCTGCCAC GCCTACGCCA CACGGGGCTG AAGGTCTACG GATCCACGTT GCTGGATGTG 
GCCTTCACCG ACTGCCTGCA GCTGGAACTG ATACAGGATG GCGCTTTTGC TAATTTAACC 
CTATTGCGAA CAATCTACAT CACCAACGCA CCCAAGCTGA CTTTCCTCTC GAAGGACGTA 
TTCCTTGGCA TTTCGGACAC CGTGGACATT ATACGAATCA TAAATAGTGG CCTGACCAGA 
GTTCCTGACT TGGGCCACCT GCCACCTCAT AATATTCTGC AAATGATAGA TCTCGATAAC 
AATCAAATCA CTCGGATAGA CAGTAAATCT ATAAAAGTAA AGACTGCCCA ATTAATTTTG 
ACAAACAATG AAATAAGCTA CGTTGATGAC TCCGCTTTCT TCGGCTCCAA GATAGCCAAA 
CTATCGCTGA AGGAAAACAA AAAACTGCAA ATGATGCATC CCAACGCATT CGATGGCATC 
ATAGACATAA CTGAACTCGA TCTCTCCAGC ACCTCGTTAG TTGGCCTTCC TTCTGCCGGA 
CTTCAGAACA TCGAAGCGTT GTACATTCAG AACACCCACA CTTTAAAGAC CATACCATCC 
ATCTACAACT TCCGGAATCT GCAGAGGGCC TATCTCACCC ACTCCTTCCA CTGCTGCGCC 
TTCCAGTTTC CATCTCGGCA CGATCCTCAG CGACACGCCC AGCGGATGCT GGAGATTGAG 
AAATGGCGGA AGCAGTGCAA AAGTGACTCC GGAACGCGAA AGGAGAGATC AACCTTGGAT 
AACCCCTTCA ATATGCCAGA AGATTTTGGT TCTTTCGGTG GCACCGACGA CTCAGCAACA 
GATATAACCC CCATCACTTT CGCTTCCTTC GATTACATGG CCGATGACAC CATGAACAAG 
GGCACTTTTC ACGAAAAGAT CATCCTGAAT CCGGGTGATG ACTCCTCGGC CGAACTTTGC 
GGCAATTTTA CTTTCAGAAA ACCCAATATT GAATGCTATC CCATGCCCAA TGATCTAAAT 
CCTTGCGAGG ATGTAATGGG CTATCAATGG CTGCGTATTT CCGTCTGGAT TGTGGTGGCT 
CTGGCCGTCG TGGGCAACGT GGCTGTGCTG ACAGTAATAC TATCGATTAG GCCGGAGTCG 
ACGCCGGTGC CGCGATTTCT GATGTGCCAC CTGGCCTTCG CCGACCTGTG CTTGGGCCTC 
TATCTGCTGC TGGTGGCCTG CATCGATGCG CATTCCATGG GGGAGTATTT CAATTTCGCC 
TACGATTGGC AGTACGGGCT TGGCTGCAAA GTAGCCGGCT TTCTCACCGT GTTCGCCAGT 
CATTTGTCGG TGTTCACGCT GACCGTGATT ACCATCGAGC GCTGGCTGGC AATCACGCAG 
GCCATGTACC TGAACCATCG CATCAAGTTG CGTCCTGCAG CGCTCATCAT GCTGGGCGGC 
TGGATTTACT CGATGCTTAT GTCCTCGCTG CCCCTGTTTG GCATAAGTAA TTATTCGTCG 
ACGAGCATTT GCCTTCCGAT GGAAAATCGC GATGTGTACG ACACCATTTA CCTGATTGCC 
ATTCTGGGCT CCAACGGAGT GGCCTTCTCC ATCATAGCCG TCTGCTATGC CCAGATCTAT 
TTGTCCTTGG GTCGCGAGAC ACGGCAGGCC CACCAGAATA GCCCGGGCGA GTTGAGCGTT 
GCCAAGAAGA TGGCCCTTTT GGTCTTTACA AACTTTGCGT GCTGGTCGCC TATTGCTTTC 
TTTGGACTCA CCGCGTTGGC CGGATACCCA CTGATTAATG TTACCAAGTC GAAGATATTG 
CTAGTCTTCT TCTATCCCTT GAATTCCTGC GCGGATCCCT ATCTGTATGC CATATTGACG 
TCCCAGTATC GCCAGGATCT GTTCACCTTG TTGTCCAAAT TGGGCCTGTG CCAGCAGAGC 
GCTCTGAAGT ACAAGGATAG CCTTTCCGGC CAAGCCACCA CCAGATTTAC AATCCACGGC 
TCCATTCAGA GGCACAGCTC ACTCACGTGC AAAATGCAGA CTGTGATGGG CGCTGAGACG 
CAGAAAATGC TGAAGAACAG CGAGGATTAT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lgr1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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