Dhx15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx15
Entrez Gene ID	35654
Full Name	DEAH-box helicase 15
Synonyms	CG11107,DHX15,Dmel\CG11107,anon-EST:Liang-1.18,cg11107,clone 1.18
General protein information	Preferred Names DEAH-box helicase 15 Names CG11107 gene product from transcript CG11107-RA CG11107-PA CG11107-PB DHX15 ortholog Dhx15-PA Dhx15-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43B2-43B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273837.1	NP_001260766.1	DEAH-box helicase 15, isoform B
NM_136425.3	NP_610269.1	DEAH-box helicase 15, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dhx15	NP_031865.2
Saccharomyces cerevisiae (baker's yeast)	PRP43	NP_011395.1
Schizosaccharomyces pombe (fission yeast)	prp43	NP_595937.1
Oryza sativa (rice)	Os03g0314100	NP_001049931.1
Rattus norvegicus (Norway rat)	Dhx15	NP_001178526.1
Gallus gallus (chicken)	DHX15	NP_001026330.1
Danio rerio (zebrafish)	dhx15	NP_001108613.1
Kluyveromyces lactis	KLLA0B00561g	XP_451555.1
Magnaporthe oryzae (rice blast fungus)	MGG_03893	XP_003719983.1
Xenopus tropicalis (tropical clawed frog)	dhx15	NP_001135571.1
Macaca mulatta (Rhesus monkey)	DHX15	XP_001106067.1
Bos taurus (cattle)	DHX15	NP_001091487.1
Caenorhabditis elegans (roundworm)	F56D2.6	NP_741147.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011149	XP_309498.4
Homo sapiens (human)	DHX15	NP_001349.2
Drosophila melanogaster (fruit fly)	CG11107	NP_610269.1
Arabidopsis thaliana (thale cress)	AT3G62310	NP_191790.1
Pan troglodytes (chimpanzee)	DHX15	XP_001164599.1
Canis lupus familiaris (dog)	DHX15	XP_851535.1
Arabidopsis thaliana (thale cress)	AT2G47250	NP_182247.1
Eremothecium gossypii	AGOS_AAR180C	NP_982723.1
Neurospora crassa	NCU01612	XP_956523.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dhx15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09150	NM_001273837.1 Latest version!	Drosophila melanogaster DEAH-box helicase 15 (Dhx15), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa09150	NM_136425.3 Latest version!	Drosophila melanogaster DEAH-box helicase 15 (Dhx15), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx15 ( NM_001273837.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260766.1 Drosophila melanogaster DEAH-box helicase 15 (Dhx15), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09150
Clone ID Related Accession (Same CDS sequence)	NM_001273837.1 , NM_136425.3
Accession Version	NM_001273837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	DEAH-box helicase 15, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGC GTCGAATCGA AGTGGGGGAG ACCTACGGCA GCAAGGCGAA GAAGGACCCG 
GAGGCGTCCT CCTCGTCGGC GGCAGCCGCT GCCGGCACAG TGGCTCAAAT CCTGGGCGGA 
TTCGTGCCCA ACAAGCAACC GCCCACGATG AACCCGCTGA CCAACACGCC GTACTCACAA 
CGGTACCAGA ATCTCTACAA GAAGCGCATT GCGCTGCCCG TGTTCGAGTA CCAGGCGGAC 
TTTATGCGGC TGCTCAGCCT GCACCAGTGT ATTGTGCTGG TGGGCGAGAC TGGCTCCGGC 
AAGACGACCC AGATCCCGCA GTGGTGCGTG GACTTCGCAG TATCCAAGGG GCGCAAGGGA 
GTCTCCTGCA CACAGCCACG TCGAGTTGCC GCCATGTCCG TGGCCCAGCG CGTTTCGGAG 
GAGATGGACG TAAAACTGGG CGAAGAGGTC GGCTACTCCA TCCGTTTCGA GGACTGCTCC 
ACGGCCAAGA CGCTGCTCAA GTACATGACC GATGGTATGT TGTTGCGCGA GGCCATGTCT 
GATCCCATGC TGGACCAGTA CCAGGTCATT CTGCTTGACG AGGCTCACGA GCGGACCTTG 
GCCACTGACA TTCTAATGGG CGTGCTCAAG GAGGTCATTC GTCAGCGCAG CGACCTTAAG 
CTGGTGGTCA TGTCCGCCAC ACTGGACGCC GGCAAGTTCC AGCAGTATTT CGACAACGCA 
CCGCTCATGA AAGTGCCCGG TCGCACACAT CCCGTGGAGA TCTTCTACAC GCCCGAACCA 
GAAAGAGATT ACCTAGAGGC TGCTATTCGC ACGGTAATCC AGATACACAT GTGCGAGGAA 
ATTGAGGGTG ATATCCTAAT GTTCCTCACG GGCCAGGAGG AGATTGAGGA GGCTTGCAAG 
CGCATCAAGC GCGAGATCGA CAATTTGGGC TCAGAAATTG GAGAGCTGAA ATGCATTCCC 
CTGTACTCGA CGCTGCCGCC CAATTTGCAG CAGCGCATCT TCGAACCGGC CCCGCCGCCA 
AATGCTAATG GCGCAATTGG ACGCAAGGTC GTGGTGTCCA CCAACATTGC CGAAACCTCG 
CTGACCATTG ACGGAGTGGT GTTCGTGATT GATCCAGGCT TCGCCAAGCA AAAGGTGTAC 
AATCCTCGCA TTCGAGTGGA GAGTCTGCTC GTCTCACCCA TTTCAAAAGC GTCTGCGCAG 
CAGAGATCCG GTCGTGCTGG ACGTACGCGT CCTGGCAAGT GTTTCCGTCT GTACACGGAA 
AAGGCGTTTA AAAACGAGAT GCAGGACAAC ACCTACCCCG AAATTCTGCG CTCCAATTTG 
GGCACTGTGG TTCTGCAGCT CAAGAAGTTG GGCATCGATG ATCTGGTGCA TTTCGATTTT 
ATGGACCCCC CTGCTCCAGA GACCTTGATG CGTGCCCTGG AGTTGCTCAA CTATCTGGCC 
GCCCTGGATG ATGACGGAAA TCTTACAGAC CTGGGTGCCG TTATGTCCGA GTTTCCATTG 
GATCCGCAGC TGGCCAAAAT GCTGATTGCC AGCTGCCAAC ATAACTGCTC TAATGAAATA 
CTTTCCATAA CGGCCATGTT GTCGGTTCCA CAATGCTTCG TTCGCCCCAA CGAGGCGAAG 
AAAGCTGCCG ATGAGGCCAA GATGCGATTC GCCCACATTG ACGGAGACCA CCTGACGTTG 
CTTAATGTCT ATCACGCCTT TAAGCAGAGC AGTGAGGATC CAAACTGGTG CTACGAGAAC 
TTCATAAACT TCCGCTCGCT AAAGAGTGCG GATAACGTGC GCCAGCAGCT GGCTAGAATC 
ATGGACCGCT TCAACTTGCG GCGCACGAGC ACTGAATTCA CCTCGAAGGA CTACTATGTA 
AACATCCGAA AGGCCCTCGT GCAGGGCTTC TTCATGCAGG TGGCCCACCT GGAGCGCACT 
GGCTACTATC TGACCATCAA GGACAACCAG AACGTGCAGC TGCATCCGTC GACTTGTCTC 
GACCACAAGC CCGATTGGGT CATCTACAAT GAGTTCGTGC TGACTACAAA GAACTACATT 
CGCACGGTGA CAGATGTCAA ACCTGAATGG TTGTGCTGCC TGGCGCCCCA ATATTATGAC 
TTGAACAACT TCCCCCAGTG CGAGGCGAAA CGACAGCTGG AGTTGTTGCA ACAGCGGTTG 
GAGACCAAGC AGTACCAGAA GGGTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260766.1
CDS	205..2394
Translation	MSKRRIEVGETYGSKAKKDPEASSSSAAAAAGTVAQILGGFVPNKQPPTMNPLTNTPYSQRYQNLYKKRIALPVFEYQADFMRLLSLHQCIVLVGETGSGKTTQIPQWCVDFAVSKGRKGVSCTQPRRVAAMSVAQRVSEEMDVKLGEEVGYSIRFEDCSTAKTLLKYMTDGMLLREAMSDPMLDQYQVILLDEAHERTLATDILMGVLKEVIRQRSDLKLVVMSATLDAGKFQQYFDNAPLMKVPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEIEGDILMFLTGQEEIEEACKRIKREIDNLGSEIGELKCIPLYSTLPPNLQQRIFEPAPPPNANGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVSPISKASAQQRSGRAGRTRPGKCFRLYTEKAFKNEMQDNTYPEILRSNLGTVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALDDDGNLTDLGAVMSEFPLDPQLAKMLIASCQHNCSNEILSITAMLSVPQCFVRPNEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQSSEDPNWCYENFINFRSLKSADNVRQQLARIMDRFNLRRTSTEFTSKDYYVNIRKALVQGFFMQVAHLERTGYYLTIKDNQNVQLHPSTCLDHKPDWVIYNEFVLTTKNYIRTVTDVKPEWLCCLAPQYYDLNNFPQCEAKRQLELLQQRLETKQYQKGF

Target ORF information:

RefSeq Version	NM_001273837.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DEAH-box helicase 15 (Dhx15), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273837.1

ORF Insert Sequence:

ATGTCCAAGC GTCGAATCGA AGTGGGGGAG ACCTACGGCA GCAAGGCGAA GAAGGACCCG 
GAGGCGTCCT CCTCGTCGGC GGCAGCCGCT GCCGGCACAG TGGCTCAAAT CCTGGGCGGA 
TTCGTGCCCA ACAAGCAACC GCCCACGATG AACCCGCTGA CCAACACGCC GTACTCACAA 
CGGTACCAGA ATCTCTACAA GAAGCGCATT GCGCTGCCCG TGTTCGAGTA CCAGGCGGAC 
TTTATGCGGC TGCTCAGCCT GCACCAGTGT ATTGTGCTGG TGGGCGAGAC TGGCTCCGGC 
AAGACGACCC AGATCCCGCA GTGGTGCGTG GACTTCGCAG TATCCAAGGG GCGCAAGGGA 
GTCTCCTGCA CACAGCCACG TCGAGTTGCC GCCATGTCCG TGGCCCAGCG CGTTTCGGAG 
GAGATGGACG TAAAACTGGG CGAAGAGGTC GGCTACTCCA TCCGTTTCGA GGACTGCTCC 
ACGGCCAAGA CGCTGCTCAA GTACATGACC GATGGTATGT TGTTGCGCGA GGCCATGTCT 
GATCCCATGC TGGACCAGTA CCAGGTCATT CTGCTTGACG AGGCTCACGA GCGGACCTTG 
GCCACTGACA TTCTAATGGG CGTGCTCAAG GAGGTCATTC GTCAGCGCAG CGACCTTAAG 
CTGGTGGTCA TGTCCGCCAC ACTGGACGCC GGCAAGTTCC AGCAGTATTT CGACAACGCA 
CCGCTCATGA AAGTGCCCGG TCGCACACAT CCCGTGGAGA TCTTCTACAC GCCCGAACCA 
GAAAGAGATT ACCTAGAGGC TGCTATTCGC ACGGTAATCC AGATACACAT GTGCGAGGAA 
ATTGAGGGTG ATATCCTAAT GTTCCTCACG GGCCAGGAGG AGATTGAGGA GGCTTGCAAG 
CGCATCAAGC GCGAGATCGA CAATTTGGGC TCAGAAATTG GAGAGCTGAA ATGCATTCCC 
CTGTACTCGA CGCTGCCGCC CAATTTGCAG CAGCGCATCT TCGAACCGGC CCCGCCGCCA 
AATGCTAATG GCGCAATTGG ACGCAAGGTC GTGGTGTCCA CCAACATTGC CGAAACCTCG 
CTGACCATTG ACGGAGTGGT GTTCGTGATT GATCCAGGCT TCGCCAAGCA AAAGGTGTAC 
AATCCTCGCA TTCGAGTGGA GAGTCTGCTC GTCTCACCCA TTTCAAAAGC GTCTGCGCAG 
CAGAGATCCG GTCGTGCTGG ACGTACGCGT CCTGGCAAGT GTTTCCGTCT GTACACGGAA 
AAGGCGTTTA AAAACGAGAT GCAGGACAAC ACCTACCCCG AAATTCTGCG CTCCAATTTG 
GGCACTGTGG TTCTGCAGCT CAAGAAGTTG GGCATCGATG ATCTGGTGCA TTTCGATTTT 
ATGGACCCCC CTGCTCCAGA GACCTTGATG CGTGCCCTGG AGTTGCTCAA CTATCTGGCC 
GCCCTGGATG ATGACGGAAA TCTTACAGAC CTGGGTGCCG TTATGTCCGA GTTTCCATTG 
GATCCGCAGC TGGCCAAAAT GCTGATTGCC AGCTGCCAAC ATAACTGCTC TAATGAAATA 
CTTTCCATAA CGGCCATGTT GTCGGTTCCA CAATGCTTCG TTCGCCCCAA CGAGGCGAAG 
AAAGCTGCCG ATGAGGCCAA GATGCGATTC GCCCACATTG ACGGAGACCA CCTGACGTTG 
CTTAATGTCT ATCACGCCTT TAAGCAGAGC AGTGAGGATC CAAACTGGTG CTACGAGAAC 
TTCATAAACT TCCGCTCGCT AAAGAGTGCG GATAACGTGC GCCAGCAGCT GGCTAGAATC 
ATGGACCGCT TCAACTTGCG GCGCACGAGC ACTGAATTCA CCTCGAAGGA CTACTATGTA 
AACATCCGAA AGGCCCTCGT GCAGGGCTTC TTCATGCAGG TGGCCCACCT GGAGCGCACT 
GGCTACTATC TGACCATCAA GGACAACCAG AACGTGCAGC TGCATCCGTC GACTTGTCTC 
GACCACAAGC CCGATTGGGT CATCTACAAT GAGTTCGTGC TGACTACAAA GAACTACATT 
CGCACGGTGA CAGATGTCAA ACCTGAATGG TTGTGCTGCC TGGCGCCCCA ATATTATGAC 
TTGAACAACT TCCCCCAGTG CGAGGCGAAA CGACAGCTGG AGTTGTTGCA ACAGCGGTTG 
GAGACCAAGC AGTACCAGAA GGGTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx15 ( NM_136425.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610269.1 Drosophila melanogaster DEAH-box helicase 15 (Dhx15), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09150
Clone ID Related Accession (Same CDS sequence)	NM_001273837.1 , NM_136425.3
Accession Version	NM_136425.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	DEAH-box helicase 15, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136425.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGC GTCGAATCGA AGTGGGGGAG ACCTACGGCA GCAAGGCGAA GAAGGACCCG 
GAGGCGTCCT CCTCGTCGGC GGCAGCCGCT GCCGGCACAG TGGCTCAAAT CCTGGGCGGA 
TTCGTGCCCA ACAAGCAACC GCCCACGATG AACCCGCTGA CCAACACGCC GTACTCACAA 
CGGTACCAGA ATCTCTACAA GAAGCGCATT GCGCTGCCCG TGTTCGAGTA CCAGGCGGAC 
TTTATGCGGC TGCTCAGCCT GCACCAGTGT ATTGTGCTGG TGGGCGAGAC TGGCTCCGGC 
AAGACGACCC AGATCCCGCA GTGGTGCGTG GACTTCGCAG TATCCAAGGG GCGCAAGGGA 
GTCTCCTGCA CACAGCCACG TCGAGTTGCC GCCATGTCCG TGGCCCAGCG CGTTTCGGAG 
GAGATGGACG TAAAACTGGG CGAAGAGGTC GGCTACTCCA TCCGTTTCGA GGACTGCTCC 
ACGGCCAAGA CGCTGCTCAA GTACATGACC GATGGTATGT TGTTGCGCGA GGCCATGTCT 
GATCCCATGC TGGACCAGTA CCAGGTCATT CTGCTTGACG AGGCTCACGA GCGGACCTTG 
GCCACTGACA TTCTAATGGG CGTGCTCAAG GAGGTCATTC GTCAGCGCAG CGACCTTAAG 
CTGGTGGTCA TGTCCGCCAC ACTGGACGCC GGCAAGTTCC AGCAGTATTT CGACAACGCA 
CCGCTCATGA AAGTGCCCGG TCGCACACAT CCCGTGGAGA TCTTCTACAC GCCCGAACCA 
GAAAGAGATT ACCTAGAGGC TGCTATTCGC ACGGTAATCC AGATACACAT GTGCGAGGAA 
ATTGAGGGTG ATATCCTAAT GTTCCTCACG GGCCAGGAGG AGATTGAGGA GGCTTGCAAG 
CGCATCAAGC GCGAGATCGA CAATTTGGGC TCAGAAATTG GAGAGCTGAA ATGCATTCCC 
CTGTACTCGA CGCTGCCGCC CAATTTGCAG CAGCGCATCT TCGAACCGGC CCCGCCGCCA 
AATGCTAATG GCGCAATTGG ACGCAAGGTC GTGGTGTCCA CCAACATTGC CGAAACCTCG 
CTGACCATTG ACGGAGTGGT GTTCGTGATT GATCCAGGCT TCGCCAAGCA AAAGGTGTAC 
AATCCTCGCA TTCGAGTGGA GAGTCTGCTC GTCTCACCCA TTTCAAAAGC GTCTGCGCAG 
CAGAGATCCG GTCGTGCTGG ACGTACGCGT CCTGGCAAGT GTTTCCGTCT GTACACGGAA 
AAGGCGTTTA AAAACGAGAT GCAGGACAAC ACCTACCCCG AAATTCTGCG CTCCAATTTG 
GGCACTGTGG TTCTGCAGCT CAAGAAGTTG GGCATCGATG ATCTGGTGCA TTTCGATTTT 
ATGGACCCCC CTGCTCCAGA GACCTTGATG CGTGCCCTGG AGTTGCTCAA CTATCTGGCC 
GCCCTGGATG ATGACGGAAA TCTTACAGAC CTGGGTGCCG TTATGTCCGA GTTTCCATTG 
GATCCGCAGC TGGCCAAAAT GCTGATTGCC AGCTGCCAAC ATAACTGCTC TAATGAAATA 
CTTTCCATAA CGGCCATGTT GTCGGTTCCA CAATGCTTCG TTCGCCCCAA CGAGGCGAAG 
AAAGCTGCCG ATGAGGCCAA GATGCGATTC GCCCACATTG ACGGAGACCA CCTGACGTTG 
CTTAATGTCT ATCACGCCTT TAAGCAGAGC AGTGAGGATC CAAACTGGTG CTACGAGAAC 
TTCATAAACT TCCGCTCGCT AAAGAGTGCG GATAACGTGC GCCAGCAGCT GGCTAGAATC 
ATGGACCGCT TCAACTTGCG GCGCACGAGC ACTGAATTCA CCTCGAAGGA CTACTATGTA 
AACATCCGAA AGGCCCTCGT GCAGGGCTTC TTCATGCAGG TGGCCCACCT GGAGCGCACT 
GGCTACTATC TGACCATCAA GGACAACCAG AACGTGCAGC TGCATCCGTC GACTTGTCTC 
GACCACAAGC CCGATTGGGT CATCTACAAT GAGTTCGTGC TGACTACAAA GAACTACATT 
CGCACGGTGA CAGATGTCAA ACCTGAATGG TTGTGCTGCC TGGCGCCCCA ATATTATGAC 
TTGAACAACT TCCCCCAGTG CGAGGCGAAA CGACAGCTGG AGTTGTTGCA ACAGCGGTTG 
GAGACCAAGC AGTACCAGAA GGGTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610269.1
CDS	342..2531
Translation	MSKRRIEVGETYGSKAKKDPEASSSSAAAAAGTVAQILGGFVPNKQPPTMNPLTNTPYSQRYQNLYKKRIALPVFEYQADFMRLLSLHQCIVLVGETGSGKTTQIPQWCVDFAVSKGRKGVSCTQPRRVAAMSVAQRVSEEMDVKLGEEVGYSIRFEDCSTAKTLLKYMTDGMLLREAMSDPMLDQYQVILLDEAHERTLATDILMGVLKEVIRQRSDLKLVVMSATLDAGKFQQYFDNAPLMKVPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEIEGDILMFLTGQEEIEEACKRIKREIDNLGSEIGELKCIPLYSTLPPNLQQRIFEPAPPPNANGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVSPISKASAQQRSGRAGRTRPGKCFRLYTEKAFKNEMQDNTYPEILRSNLGTVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALDDDGNLTDLGAVMSEFPLDPQLAKMLIASCQHNCSNEILSITAMLSVPQCFVRPNEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQSSEDPNWCYENFINFRSLKSADNVRQQLARIMDRFNLRRTSTEFTSKDYYVNIRKALVQGFFMQVAHLERTGYYLTIKDNQNVQLHPSTCLDHKPDWVIYNEFVLTTKNYIRTVTDVKPEWLCCLAPQYYDLNNFPQCEAKRQLELLQQRLETKQYQKGF

Target ORF information:

RefSeq Version	NM_136425.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DEAH-box helicase 15 (Dhx15), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136425.3

ORF Insert Sequence:

ATGTCCAAGC GTCGAATCGA AGTGGGGGAG ACCTACGGCA GCAAGGCGAA GAAGGACCCG 
GAGGCGTCCT CCTCGTCGGC GGCAGCCGCT GCCGGCACAG TGGCTCAAAT CCTGGGCGGA 
TTCGTGCCCA ACAAGCAACC GCCCACGATG AACCCGCTGA CCAACACGCC GTACTCACAA 
CGGTACCAGA ATCTCTACAA GAAGCGCATT GCGCTGCCCG TGTTCGAGTA CCAGGCGGAC 
TTTATGCGGC TGCTCAGCCT GCACCAGTGT ATTGTGCTGG TGGGCGAGAC TGGCTCCGGC 
AAGACGACCC AGATCCCGCA GTGGTGCGTG GACTTCGCAG TATCCAAGGG GCGCAAGGGA 
GTCTCCTGCA CACAGCCACG TCGAGTTGCC GCCATGTCCG TGGCCCAGCG CGTTTCGGAG 
GAGATGGACG TAAAACTGGG CGAAGAGGTC GGCTACTCCA TCCGTTTCGA GGACTGCTCC 
ACGGCCAAGA CGCTGCTCAA GTACATGACC GATGGTATGT TGTTGCGCGA GGCCATGTCT 
GATCCCATGC TGGACCAGTA CCAGGTCATT CTGCTTGACG AGGCTCACGA GCGGACCTTG 
GCCACTGACA TTCTAATGGG CGTGCTCAAG GAGGTCATTC GTCAGCGCAG CGACCTTAAG 
CTGGTGGTCA TGTCCGCCAC ACTGGACGCC GGCAAGTTCC AGCAGTATTT CGACAACGCA 
CCGCTCATGA AAGTGCCCGG TCGCACACAT CCCGTGGAGA TCTTCTACAC GCCCGAACCA 
GAAAGAGATT ACCTAGAGGC TGCTATTCGC ACGGTAATCC AGATACACAT GTGCGAGGAA 
ATTGAGGGTG ATATCCTAAT GTTCCTCACG GGCCAGGAGG AGATTGAGGA GGCTTGCAAG 
CGCATCAAGC GCGAGATCGA CAATTTGGGC TCAGAAATTG GAGAGCTGAA ATGCATTCCC 
CTGTACTCGA CGCTGCCGCC CAATTTGCAG CAGCGCATCT TCGAACCGGC CCCGCCGCCA 
AATGCTAATG GCGCAATTGG ACGCAAGGTC GTGGTGTCCA CCAACATTGC CGAAACCTCG 
CTGACCATTG ACGGAGTGGT GTTCGTGATT GATCCAGGCT TCGCCAAGCA AAAGGTGTAC 
AATCCTCGCA TTCGAGTGGA GAGTCTGCTC GTCTCACCCA TTTCAAAAGC GTCTGCGCAG 
CAGAGATCCG GTCGTGCTGG ACGTACGCGT CCTGGCAAGT GTTTCCGTCT GTACACGGAA 
AAGGCGTTTA AAAACGAGAT GCAGGACAAC ACCTACCCCG AAATTCTGCG CTCCAATTTG 
GGCACTGTGG TTCTGCAGCT CAAGAAGTTG GGCATCGATG ATCTGGTGCA TTTCGATTTT 
ATGGACCCCC CTGCTCCAGA GACCTTGATG CGTGCCCTGG AGTTGCTCAA CTATCTGGCC 
GCCCTGGATG ATGACGGAAA TCTTACAGAC CTGGGTGCCG TTATGTCCGA GTTTCCATTG 
GATCCGCAGC TGGCCAAAAT GCTGATTGCC AGCTGCCAAC ATAACTGCTC TAATGAAATA 
CTTTCCATAA CGGCCATGTT GTCGGTTCCA CAATGCTTCG TTCGCCCCAA CGAGGCGAAG 
AAAGCTGCCG ATGAGGCCAA GATGCGATTC GCCCACATTG ACGGAGACCA CCTGACGTTG 
CTTAATGTCT ATCACGCCTT TAAGCAGAGC AGTGAGGATC CAAACTGGTG CTACGAGAAC 
TTCATAAACT TCCGCTCGCT AAAGAGTGCG GATAACGTGC GCCAGCAGCT GGCTAGAATC 
ATGGACCGCT TCAACTTGCG GCGCACGAGC ACTGAATTCA CCTCGAAGGA CTACTATGTA 
AACATCCGAA AGGCCCTCGT GCAGGGCTTC TTCATGCAGG TGGCCCACCT GGAGCGCACT 
GGCTACTATC TGACCATCAA GGACAACCAG AACGTGCAGC TGCATCCGTC GACTTGTCTC 
GACCACAAGC CCGATTGGGT CATCTACAAT GAGTTCGTGC TGACTACAAA GAACTACATT 
CGCACGGTGA CAGATGTCAA ACCTGAATGG TTGTGCTGCC TGGCGCCCCA ATATTATGAC 
TTGAACAACT TCCCCCAGTG CGAGGCGAAA CGACAGCTGG AGTTGTTGCA ACAGCGGTTG 
GAGACCAAGC AGTACCAGAA GGGTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dhx15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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