DHX15 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX15
Entrez Gene ID	1665
Full Name	DEAH-box helicase 15
Synonyms	DBP1,DDX15,HRH2,PRP43,PRPF43,PrPp43p
General protein information	Preferred Names DEAH-box helicase 15 Names pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 ATP-dependent RNA helicase #46 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15 DEAD/H box-15 DEAH (Asp-Glu-Ala-His) box helicase 15 DEAH (Asp-Glu-Ala-His) box polypeptide 15 DEAH box protein 15 RNA helicase 2 putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p15.2
Summary	The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing. [provided by RefSeq, Jul 2008].
Disorder MIM:	603403

mRNA and Protein(s)

mRNA	Protein	Name
NM_001358.2	NP_001349.2	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
NM_001358.3	NP_001349.2	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dhx15	NP_031865.2
Saccharomyces cerevisiae (baker's yeast)	PRP43	NP_011395.1
Schizosaccharomyces pombe (fission yeast)	prp43	NP_595937.1
Oryza sativa (rice)	Os03g0314100	NP_001049931.1
Rattus norvegicus (Norway rat)	Dhx15	NP_001178526.1
Gallus gallus (chicken)	DHX15	NP_001026330.1
Danio rerio (zebrafish)	dhx15	NP_001108613.1
Kluyveromyces lactis	KLLA0B00561g	XP_451555.1
Magnaporthe oryzae (rice blast fungus)	MGG_03893	XP_003719983.1
Xenopus tropicalis (tropical clawed frog)	dhx15	NP_001135571.1
Macaca mulatta (Rhesus monkey)	DHX15	XP_001106067.1
Bos taurus (cattle)	DHX15	NP_001091487.1
Caenorhabditis elegans (roundworm)	F56D2.6	NP_741147.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011149	XP_309498.4
Homo sapiens (human)	DHX15	NP_001349.2
Drosophila melanogaster (fruit fly)	CG11107	NP_610269.1
Arabidopsis thaliana (thale cress)	AT3G62310	NP_191790.1
Pan troglodytes (chimpanzee)	DHX15	XP_001164599.1
Canis lupus familiaris (dog)	DHX15	XP_851535.1
Arabidopsis thaliana (thale cress)	AT2G47250	NP_182247.1
Eremothecium gossypii	AGOS_AAR180C	NP_982723.1
Neurospora crassa	NCU01612	XP_956523.2

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression and clinical relevance of the RNA helicase DHX15 in hepatocellular carcinoma.
Xie C, Liao H, Zhang C, Zhang S
Human pathology84213-220(2019 02)

Human RNA cap1 methyltransferase CMTr1 cooperates with RNA helicase DHX15 to modify RNAs with highly structured 5' termini.
Toczydlowska-Socha D, Zielinska MM, Kurkowska M, Astha, Almeida CF, Stefaniak F, Purta E, Bujnicki JM
Philosophical transactions of the Royal Society of London. Series B, Biological sciences373(1762)(2018 11)

ETS1 and SP1 drive DHX15 expression in acute lymphoblastic leukaemia.
Chen XL, Cai YH, Liu Q, Pan LL, Shi SL, Liu XL, Chen Y, Li JG, Wang J, Li Y, Li XF, Wang SY
Journal of cellular and molecular medicine22(5)2612-2621(2018 05)

DHX15 promotes prostate cancer progression by stimulating Siah2-mediated ubiquitination of androgen receptor.
Jing Y, Nguyen MM, Wang D, Pascal LE, Guo W, Xu Y, Ai J, Deng FM, Masoodi KZ, Yu X, Zhang J, Nelson JB, Xia S, Wang Z
Oncogene37(5)638-650(2018 02)

Protein interaction network of alternatively spliced NudCD1 isoforms.
Asselin-Mullen P, Chauvin A, Dubois ML, Drissi R, Lévesque D, Boisvert FM
Scientific reports7(1)12987(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DHX15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16259	NM_001358.3 Latest version!	Homo sapiens DEAH-box helicase 15 (DHX15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu16259	NM_001358.2	Homo sapiens DEAH-box helicase 15 (DHX15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX15 ( NM_001358.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349.2 Homo sapiens DEAH-box helicase 15 (DHX15), mRNA.

Price & Availability↑

CloneID	OHu16259
Clone ID Related Accession (Same CDS sequence)	NM_001358.2 , NM_001358.3
Accession Version	NM_001358.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512835200000
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU158645.1, BC035974.1 and AA771922.1. On Jul 1, 2005 this sequence version replaced NM_001358.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB001636.1, SRR1803612.268986.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGC GGCACCGGTT GGACCTAGGG GAGGATTACC CCTCTGGCAA GAAGCGTGCG 
GGGACCGATG GGAAGGATCG AGATCGAGAC CGGGATCGTG AAGATCGGTC TAAAGATCGA 
GACCGAGAAC GTGATAGAGG AGATAGAGAG CGAGAGAGGG AGAAAGAAAA GGAGAAGGAG 
TTGCGAGCTT CAACAAATGC TATGCTTATC AGTGCTGGAT TACCACCTTT GAAAGCTTCC 
CATTCAGCTC ACTCAACCCA CTCAGCACAT TCAACGCATT CAACACATTC TGCTCATTCA 
ACGCATGCCG GACATGCAGG TCACACGTCA CTTCCACAGT GCATTAATCC GTTCACCAAC 
TTACCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GTCTTCAGCT CCCTGTTTGG 
GAATACAAGG ATAGGTTTAC AGATATTCTG GTTAGACATC AGTCCTTTGT ACTGGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATT CCACAGTGGT GTGTGGAGTA CATGCGATCA 
TTACCAGGAC CCAAGAGAGG AGTTGCCTGT ACCCAACCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAT GTGATGTTGG GCCAGGAAGT TGGTTACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGATGGGATG 
TTACTTCGTG AAGCTATGAA TGATCCCCTC CTGGAGCGTT ATGGTGTAAT AATTCTTGAT 
GAGGCTCATG AGAGGACACT GGCTACAGAT ATTCTAATGG GTGTTCTGAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGCGCTA CTCTAGATGC AGGAAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCCTA ACTATTCCTG GGCGTACACA TCCTGTTGAG 
ATCTTCTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG AACAGTTATC 
CAGATTCATA TGTGTGAAGA GGAAGAGGGA GATCTTCTTC TTTTCTTAAC TGGTCAAGAG 
GAAATTGATG AAGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGACATTA AAATCATTCC ATTGTATTCT ACACTTCCAC CTCAGCAGCA GCAACGCATT 
TTTGAGCCTC CACCTCCCAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT AGTTGTGTCA 
ACTAACATAG CAGAGACGTC TTTGACAATA GATGGTGTGG TGTTTGTGAT TGATCCTGGA 
TTTGCGAAAC AGAAGGTCTA CAATCCTCGA ATCAGAGTTG AGTCCCTTTT GGTGACAGCT 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCT GGTCGAGCTG GACGTACCAG ACCTGGAAAA 
TGCTTCAGAC TTTACACAGA GAAAGCTTAT AAAACAGAAA TGCAGGATAA CACCTATCCT 
GAGATTTTGC GTTCTAATTT AGGATCAGTT GTGTTACAAT TGAAGAAACT TGGTATTGAT 
GACTTGGTAC ATTTTGATTT TATGGATCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTG 
GAACTTTTGA ATTACCTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTTCCTCT AGATCCACAG CTCGCAAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCCGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAACGTC TACCATGCTT TTAAACAAAA TCATGAATCG 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACAGGTCCC TGATGTCCGC AGACAATGTA 
CGCCAGCAGC TATCTCGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGACTTT 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAG 
GTGGCACATT TAGAACGAAC AGGGCATTAC TTAACTGTGA AAGATAACCA GGTGGTTCAG 
TTGCATCCCT CTACTGTTCT TGACCACAAA CCTGAATGGG TGCTTTATAA TGAGTTTGTT 
CTAACAACAA AGAATTACAT CCGGACATGT ACAGATATCA AGCCAGAATG GTTGGTGAAA 
ATTGCCCCTC AATATTATGA CATGAGCAAT TTCCCACAGT GTGAAGCAAA GAGACAGTTG 
GACCGCATCA TTGCCAAACT TCAATCCAAG GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349.2
CDS	169..2556
Translation	MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQSKEYSQY

Target ORF information:

RefSeq Version	NM_001358.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 15 (DHX15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358.2

ORF Insert Sequence:

ATGTCCAAGC GGCACCGGTT GGACCTAGGG GAGGATTACC CCTCTGGCAA GAAGCGTGCG 
GGGACCGATG GGAAGGATCG AGATCGAGAC CGGGATCGTG AAGATCGGTC TAAAGATCGA 
GACCGAGAAC GTGATAGAGG AGATAGAGAG CGAGAGAGGG AGAAAGAAAA GGAGAAGGAG 
TTGCGAGCTT CAACAAATGC TATGCTTATC AGTGCTGGAT TACCACCTTT GAAAGCTTCC 
CATTCAGCTC ACTCAACCCA CTCAGCACAT TCAACGCATT CAACACATTC TGCTCATTCA 
ACGCATGCCG GACATGCAGG TCACACGTCA CTTCCACAGT GCATTAATCC GTTCACCAAC 
TTACCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GTCTTCAGCT CCCTGTTTGG 
GAATACAAGG ATAGGTTTAC AGATATTCTG GTTAGACATC AGTCCTTTGT ACTGGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATT CCACAGTGGT GTGTGGAGTA CATGCGATCA 
TTACCAGGAC CCAAGAGAGG AGTTGCCTGT ACCCAACCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAT GTGATGTTGG GCCAGGAAGT TGGTTACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGATGGGATG 
TTACTTCGTG AAGCTATGAA TGATCCCCTC CTGGAGCGTT ATGGTGTAAT AATTCTTGAT 
GAGGCTCATG AGAGGACACT GGCTACAGAT ATTCTAATGG GTGTTCTGAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGCGCTA CTCTAGATGC AGGAAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCCTA ACTATTCCTG GGCGTACACA TCCTGTTGAG 
ATCTTCTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG AACAGTTATC 
CAGATTCATA TGTGTGAAGA GGAAGAGGGA GATCTTCTTC TTTTCTTAAC TGGTCAAGAG 
GAAATTGATG AAGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGACATTA AAATCATTCC ATTGTATTCT ACACTTCCAC CTCAGCAGCA GCAACGCATT 
TTTGAGCCTC CACCTCCCAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT AGTTGTGTCA 
ACTAACATAG CAGAGACGTC TTTGACAATA GATGGTGTGG TGTTTGTGAT TGATCCTGGA 
TTTGCGAAAC AGAAGGTCTA CAATCCTCGA ATCAGAGTTG AGTCCCTTTT GGTGACAGCT 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCT GGTCGAGCTG GACGTACCAG ACCTGGAAAA 
TGCTTCAGAC TTTACACAGA GAAAGCTTAT AAAACAGAAA TGCAGGATAA CACCTATCCT 
GAGATTTTGC GTTCTAATTT AGGATCAGTT GTGTTACAAT TGAAGAAACT TGGTATTGAT 
GACTTGGTAC ATTTTGATTT TATGGATCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTG 
GAACTTTTGA ATTACCTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTTCCTCT AGATCCACAG CTCGCAAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCCGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAACGTC TACCATGCTT TTAAACAAAA TCATGAATCG 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACAGGTCCC TGATGTCCGC AGACAATGTA 
CGCCAGCAGC TATCTCGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGACTTT 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAG 
GTGGCACATT TAGAACGAAC AGGGCATTAC TTAACTGTGA AAGATAACCA GGTGGTTCAG 
TTGCATCCCT CTACTGTTCT TGACCACAAA CCTGAATGGG TGCTTTATAA TGAGTTTGTT 
CTAACAACAA AGAATTACAT CCGGACATGT ACAGATATCA AGCCAGAATG GTTGGTGAAA 
ATTGCCCCTC AATATTATGA CATGAGCAAT TTCCCACAGT GTGAAGCAAA GAGACAGTTG 
GACCGCATCA TTGCCAAACT TCAATCCAAG GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX15 ( NM_001358.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349.2 Homo sapiens DEAH-box helicase 15 (DHX15), mRNA.

Price & Availability↑

CloneID	OHu16259
Clone ID Related Accession (Same CDS sequence)	NM_001358.2 , NM_001358.3
Accession Version	NM_001358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574092800000
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU158645.1, BC035974.1 and AA771922.1. On Nov 22, 2018 this sequence version replaced NM_001358.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB001636.1, SRR3476690.1166976.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336812.5/ ENSP00000336741.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCAAGC GGCACCGGTT GGACCTAGGG GAGGATTACC CCTCTGGCAA GAAGCGTGCG 
GGGACCGATG GGAAGGATCG AGATCGAGAC CGGGATCGTG AAGATCGGTC TAAAGATCGA 
GACCGAGAAC GTGATAGAGG AGATAGAGAG CGAGAGAGGG AGAAAGAAAA GGAGAAGGAG 
TTGCGAGCTT CAACAAATGC TATGCTTATC AGTGCTGGAT TACCACCTTT GAAAGCTTCC 
CATTCAGCTC ACTCAACCCA CTCAGCACAT TCAACGCATT CAACACATTC TGCTCATTCA 
ACGCATGCCG GACATGCAGG TCACACGTCA CTTCCACAGT GCATTAATCC GTTCACCAAC 
TTACCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GTCTTCAGCT CCCTGTTTGG 
GAATACAAGG ATAGGTTTAC AGATATTCTG GTTAGACATC AGTCCTTTGT ACTGGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATT CCACAGTGGT GTGTGGAGTA CATGCGATCA 
TTACCAGGAC CCAAGAGAGG AGTTGCCTGT ACCCAACCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAT GTGATGTTGG GCCAGGAAGT TGGTTACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGATGGGATG 
TTACTTCGTG AAGCTATGAA TGATCCCCTC CTGGAGCGTT ATGGTGTAAT AATTCTTGAT 
GAGGCTCATG AGAGGACACT GGCTACAGAT ATTCTAATGG GTGTTCTGAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGCGCTA CTCTAGATGC AGGAAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCCTA ACTATTCCTG GGCGTACACA TCCTGTTGAG 
ATCTTCTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG AACAGTTATC 
CAGATTCATA TGTGTGAAGA GGAAGAGGGA GATCTTCTTC TTTTCTTAAC TGGTCAAGAG 
GAAATTGATG AAGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGACATTA AAATCATTCC ATTGTATTCT ACACTTCCAC CTCAGCAGCA GCAACGCATT 
TTTGAGCCTC CACCTCCCAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT AGTTGTGTCA 
ACTAACATAG CAGAGACGTC TTTGACAATA GATGGTGTGG TGTTTGTGAT TGATCCTGGA 
TTTGCGAAAC AGAAGGTCTA CAATCCTCGA ATCAGAGTTG AGTCCCTTTT GGTGACAGCT 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCT GGTCGAGCTG GACGTACCAG ACCTGGAAAA 
TGCTTCAGAC TTTACACAGA GAAAGCTTAT AAAACAGAAA TGCAGGATAA CACCTATCCT 
GAGATTTTGC GTTCTAATTT AGGATCAGTT GTGTTACAAT TGAAGAAACT TGGTATTGAT 
GACTTGGTAC ATTTTGATTT TATGGATCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTG 
GAACTTTTGA ATTACCTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTTCCTCT AGATCCACAG CTCGCAAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCCGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAACGTC TACCATGCTT TTAAACAAAA TCATGAATCG 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACAGGTCCC TGATGTCCGC AGACAATGTA 
CGCCAGCAGC TATCTCGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGACTTT 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAG 
GTGGCACATT TAGAACGAAC AGGGCATTAC TTAACTGTGA AAGATAACCA GGTGGTTCAG 
TTGCATCCCT CTACTGTTCT TGACCACAAA CCTGAATGGG TGCTTTATAA TGAGTTTGTT 
CTAACAACAA AGAATTACAT CCGGACATGT ACAGATATCA AGCCAGAATG GTTGGTGAAA 
ATTGCCCCTC AATATTATGA CATGAGCAAT TTCCCACAGT GTGAAGCAAA GAGACAGTTG 
GACCGCATCA TTGCCAAACT TCAATCCAAG GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349.2
CDS	162..2549
Translation	MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQSKEYSQY

Target ORF information:

RefSeq Version	NM_001358.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 15 (DHX15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358.3

ORF Insert Sequence:

ATGTCCAAGC GGCACCGGTT GGACCTAGGG GAGGATTACC CCTCTGGCAA GAAGCGTGCG 
GGGACCGATG GGAAGGATCG AGATCGAGAC CGGGATCGTG AAGATCGGTC TAAAGATCGA 
GACCGAGAAC GTGATAGAGG AGATAGAGAG CGAGAGAGGG AGAAAGAAAA GGAGAAGGAG 
TTGCGAGCTT CAACAAATGC TATGCTTATC AGTGCTGGAT TACCACCTTT GAAAGCTTCC 
CATTCAGCTC ACTCAACCCA CTCAGCACAT TCAACGCATT CAACACATTC TGCTCATTCA 
ACGCATGCCG GACATGCAGG TCACACGTCA CTTCCACAGT GCATTAATCC GTTCACCAAC 
TTACCCCATA CTCCTCGATA CTATGATATT CTAAAGAAAC GTCTTCAGCT CCCTGTTTGG 
GAATACAAGG ATAGGTTTAC AGATATTCTG GTTAGACATC AGTCCTTTGT ACTGGTTGGT 
GAGACTGGGT CTGGTAAAAC AACACAGATT CCACAGTGGT GTGTGGAGTA CATGCGATCA 
TTACCAGGAC CCAAGAGAGG AGTTGCCTGT ACCCAACCCA GGAGAGTGGC TGCAATGAGT 
GTGGCTCAGA GAGTTGCTGA TGAGATGGAT GTGATGTTGG GCCAGGAAGT TGGTTACTCC 
ATTCGATTTG AAGACTGCAG TAGTGCAAAA ACCATTCTTA AGTATATGAC TGATGGGATG 
TTACTTCGTG AAGCTATGAA TGATCCCCTC CTGGAGCGTT ATGGTGTAAT AATTCTTGAT 
GAGGCTCATG AGAGGACACT GGCTACAGAT ATTCTAATGG GTGTTCTGAA GGAAGTTGTA 
AGACAGAGAT CAGATTTAAA GGTTATAGTT ATGAGCGCTA CTCTAGATGC AGGAAAATTC 
CAGATTTACT TTGATAACTG TCCTCTCCTA ACTATTCCTG GGCGTACACA TCCTGTTGAG 
ATCTTCTATA CTCCAGAACC AGAGAGAGAT TATCTTGAAG CAGCAATTCG AACAGTTATC 
CAGATTCATA TGTGTGAAGA GGAAGAGGGA GATCTTCTTC TTTTCTTAAC TGGTCAAGAG 
GAAATTGATG AAGCCTGTAA GAGAATAAAG CGTGAAGTTG ATGATTTGGG CCCTGAAGTT 
GGTGACATTA AAATCATTCC ATTGTATTCT ACACTTCCAC CTCAGCAGCA GCAACGCATT 
TTTGAGCCTC CACCTCCCAA AAAACAGAAT GGAGCAATTG GAAGAAAGGT AGTTGTGTCA 
ACTAACATAG CAGAGACGTC TTTGACAATA GATGGTGTGG TGTTTGTGAT TGATCCTGGA 
TTTGCGAAAC AGAAGGTCTA CAATCCTCGA ATCAGAGTTG AGTCCCTTTT GGTGACAGCT 
ATTAGTAAAG CTTCAGCTCA GCAAAGGGCT GGTCGAGCTG GACGTACCAG ACCTGGAAAA 
TGCTTCAGAC TTTACACAGA GAAAGCTTAT AAAACAGAAA TGCAGGATAA CACCTATCCT 
GAGATTTTGC GTTCTAATTT AGGATCAGTT GTGTTACAAT TGAAGAAACT TGGTATTGAT 
GACTTGGTAC ATTTTGATTT TATGGATCCA CCAGCTCCTG AAACTCTGAT GAGAGCCCTG 
GAACTTTTGA ATTACCTGGC TGCTTTAAAT GATGATGGAG ATCTGACTGA ATTGGGATCC 
ATGATGGCAG AGTTTCCTCT AGATCCACAG CTCGCAAAAA TGGTTATTGC AAGTTGTGAC 
TACAACTGTT CTAATGAGGT CCTATCTATT ACTGCTATGT TGTCAGTCCC ACAGTGTTTT 
GTTCGCCCCA CGGAGGCCAA GAAAGCCGCA GATGAGGCCA AGATGAGATT TGCCCACATA 
GATGGAGATC ATCTGACACT GCTGAACGTC TACCATGCTT TTAAACAAAA TCATGAATCG 
GTTCAGTGGT GTTATGACAA CTTCATTAAC TACAGGTCCC TGATGTCCGC AGACAATGTA 
CGCCAGCAGC TATCTCGAAT TATGGACAGA TTTAATTTGC CTCGTCGAAG TACTGACTTT 
ACAAGCAGGG ACTATTATAT TAATATAAGA AAAGCTTTGG TTACTGGGTA TTTTATGCAG 
GTGGCACATT TAGAACGAAC AGGGCATTAC TTAACTGTGA AAGATAACCA GGTGGTTCAG 
TTGCATCCCT CTACTGTTCT TGACCACAAA CCTGAATGGG TGCTTTATAA TGAGTTTGTT 
CTAACAACAA AGAATTACAT CCGGACATGT ACAGATATCA AGCCAGAATG GTTGGTGAAA 
ATTGCCCCTC AATATTATGA CATGAGCAAT TTCCCACAGT GTGAAGCAAA GAGACAGTTG 
GACCGCATCA TTGCCAAACT TCAATCCAAG GAATATTCAC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression and clinical relevance of the RNA helicase DHX15 in hepatocellular carcinoma.
Human pathology84213-220(2019 02)
Xie C,Liao H,Zhang C,Zhang S

Human RNA cap1 methyltransferase CMTr1 cooperates with RNA helicase DHX15 to modify RNAs with highly structured 5' termini.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences373(1762)(2018 11)
Toczydlowska-Socha D,Zielinska MM,Kurkowska M,Astha,Almeida CF,Stefaniak F,Purta E,Bujnicki JM

ETS1 and SP1 drive DHX15 expression in acute lymphoblastic leukaemia.
Journal of cellular and molecular medicine22(5)2612-2621(2018 05)
Chen XL,Cai YH,Liu Q,Pan LL,Shi SL,Liu XL,Chen Y,Li JG,Wang J,Li Y,Li XF,Wang SY

DHX15 promotes prostate cancer progression by stimulating Siah2-mediated ubiquitination of androgen receptor.
Oncogene37(5)638-650(2018 02)
Jing Y,Nguyen MM,Wang D,Pascal LE,Guo W,Xu Y,Ai J,Deng FM,Masoodi KZ,Yu X,Zhang J,Nelson JB,Xia S,Wang Z

Protein interaction network of alternatively spliced NudCD1 isoforms.
Scientific reports7(1)12987(2017 10)
Asselin-Mullen P,Chauvin A,Dubois ML,Drissi R,Lévesque D,Boisvert FM

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DHX15 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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