SI:DKEY-47K20.9 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:DKEY-47K20.9
Entrez Gene ID	792647
Full Name	si:dkey-47k20.9
General protein information	Preferred Names si:dkey-47k20.9 Names extracellular calcium-sensing receptor
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001332178.6	XP_001332214.6	extracellular calcium-sensing receptor isoform X1
XM_001332178.7	XP_001332214.6	extracellular calcium-sensing receptor isoform X1
XM_009293764.1	XP_009292039.1	extracellular calcium-sensing receptor isoform X3
XM_009293762.2	XP_009292037.1	extracellular calcium-sensing receptor isoform X2
XM_009293762.1	XP_009292037.1	extracellular calcium-sensing receptor isoform X2
XM_009293765.1	XP_009292040.1	extracellular calcium-sensing receptor isoform X4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	olfcw1	NP_001018157.1
Danio rerio (zebrafish)	LOC794788	XP_001334811.5
Danio rerio (zebrafish)	v2ra18	XP_697465.5
Danio rerio (zebrafish)	v2rh11	XP_002665412.2
Danio rerio (zebrafish)	v2rh10	XP_001333853.3
Danio rerio (zebrafish)	v2rh7	NP_001119923.1
Danio rerio (zebrafish)	si:dkey-47k20.1	XP_684062.4
Danio rerio (zebrafish)	si:dkey-47k20.3	XP_001332817.5
Danio rerio (zebrafish)	olfcg9	XP_005158744.1
Danio rerio (zebrafish)	LOC100007210	XP_001345723.5
Danio rerio (zebrafish)	LOC100007225	XP_001345735.3
Danio rerio (zebrafish)	LOC101884304	XP_005159271.1
Danio rerio (zebrafish)	LOC100149406	NP_001153313.1
Danio rerio (zebrafish)	olfcg8	XP_001919026.3
Danio rerio (zebrafish)	v2rh32	NP_001076358.1
Danio rerio (zebrafish)	v2rh12	XP_685037.4
Danio rerio (zebrafish)	LOC562852	NP_001121719.1
Danio rerio (zebrafish)	v2rh14	NP_001076568.1
Danio rerio (zebrafish)	olfch1	NP_001103760.1
Danio rerio (zebrafish)	LOC100007121	XP_001345668.3
Danio rerio (zebrafish)	LOC100148688	XP_001919391.3
Danio rerio (zebrafish)	si:ch211-229c8.14	XP_003200514.2
Danio rerio (zebrafish)	v2rc2	XP_001335066.3
Danio rerio (zebrafish)	LOC793310	XP_001333050.5
Danio rerio (zebrafish)	olfcg6	XP_005159270.1
Danio rerio (zebrafish)	v2ra16	XP_001334567.1
Danio rerio (zebrafish)	si:dkey-47k20.4	XP_001332729.4
Danio rerio (zebrafish)	v2rh1	XP_684341.5
Danio rerio (zebrafish)	olfcg3	NP_001103412.1
Danio rerio (zebrafish)	olfcg4	NP_001122034.1
Danio rerio (zebrafish)	LOC100537475	XP_003200513.1
Danio rerio (zebrafish)	LOC793043	XP_001332717.1
Danio rerio (zebrafish)	olfcs2	NP_001094425.1
Danio rerio (zebrafish)	v2ra17	XP_696250.6
Danio rerio (zebrafish)	v2rh9	XP_001333318.3
Danio rerio (zebrafish)	si:dkey-47k20.8	XP_683668.5
Danio rerio (zebrafish)	si:dkey-47k20.9	XP_001332214.5
Danio rerio (zebrafish)	LOC101882886	XP_005159267.1
Danio rerio (zebrafish)	olfcg2	NP_001103759.1
Danio rerio (zebrafish)	olfcg1	NP_001153312.1
Danio rerio (zebrafish)	LOC100148278	XP_001919635.1
Danio rerio (zebrafish)	v2ra1	XP_001919102.4
Danio rerio (zebrafish)	v2rh13	XP_685124.5
Danio rerio (zebrafish)	si:dkey-47k20.2	XP_683954.4
Danio rerio (zebrafish)	v2rx1	NP_001076368.1
Danio rerio (zebrafish)	olfcs1	NP_001038510.1
Danio rerio (zebrafish)	olfcd3	XP_696754.5
Danio rerio (zebrafish)	olfcd1	XP_001335160.3
Danio rerio (zebrafish)	olfcd2	NP_001074079.1
Danio rerio (zebrafish)	v2rc1	XP_001335414.4
Danio rerio (zebrafish)	si:dkey-47k20.7	XP_699729.3
Danio rerio (zebrafish)	si:dkey-47k20.5	XP_001332644.4
Danio rerio (zebrafish)	olfcq19	NP_001077346.1
Danio rerio (zebrafish)	si:ch211-203b20.4	XP_003200515.2
Danio rerio (zebrafish)	olfcg5	NP_001103413.1
Danio rerio (zebrafish)	olfcj1	NP_001103761.1

Gene Ontology

The following SI:DKEY-47K20.9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:DKEY-47K20.9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa46705	XM_001332178.7 Latest version!	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa46706	XM_009293762.2 Latest version!	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa46708	XM_009293764.1 Latest version!	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa46709	XM_009293765.1 Latest version!	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa46705	XM_001332178.6	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa46706	XM_009293762.1	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:DKEY-47K20.9 ( XM_001332178.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001332214.6 Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa46705
Clone ID Related Accession (Same CDS sequence)	XM_001332178.6 , XM_001332178.7
Accession Version	XM_001332178.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	extracellular calcium-sensing receptor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_001332178.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGGTACTCAC ACTGGATGAA GATGCAATAT TCTGGAATTT TGAGACGAGA 
AAACCCCCAC GGTCTGTGTG CAGTGAAAGC TGCCCTCCAG GCACCAGACG AGCCACTCGC 
AAGGGTCTTC CTGTCTGCTG TTTTGACTGC CTGCCATGTG GAGATGGAGA GATTTCTAAC 
ACAACAAATG CTGTTGAGTG CTTTCTATGT CCAGATGAGT TTTGGTCCAA TCAATATAAT 
AATCACTGTG TACCAAAAGA AGTAGAGTTT CTCTCCTATG AAGATCCTCT GGGGATCTCC 
CTGACCACTG CTTCTCTGCT TGGCACCTGC TTCTGTGCTC TTGTGATATT TGTTTTTGCT 
CTTCATCGTA ATACTCCTAT AGTACGTGCA AATAATTCAG AGCTCAGCTT CCTACTGCTG 
CTGTCACTCA AACTGTGTTT CCTTTGTGTC CTGCTGTTTA TTGGTCGACC ACAGCTGTGG 
ACATGTCAGT TAAGACATGT TGTGTTTGGT ATAAGCTTTG TCCTGTGCAT GTCCAGTATT 
CTGGTCAAGA CTATGGTGGT AATAGCTGTG TTCAAGTCCT CTCGGCCAGA AGGCAAAGGA 
GCAATAAAAT GGTTTGGAGC AGTTCAACAG AGAAGCACAG TTGTTGTCCT CACAGTCCTC 
CAGGTTCTTA TATGTGCAGT CTGGTTATCA ACTGCCTCTC CAACACCCTA TAAAAACAAC 
CAATATATCC GTTCTAAAAT AGTATATGAA TGTGCTATAG GTTCAGTTGT TGGGTTTTCA 
ATGTTACTCA GCTATATTGG CTTTTTGGCA GCAGCCAGCT TTTTGTTAGC CTTCCTGGCT 
AGAAATCTTC CAGATAATTT TAATGAAGCA AAGTTCATCA CATTTAGCAT GTTGATTTTT 
AGTGCCGTGT GGATTGCATT TGTTCCAGCG TATGTGAGTT CACCAGGTAA ATACACAGTG 
GCTGTAGAAA TTTTCGCCAT TCTAGCTTCG ACTTTTGGTT TACTGATTGC CATATTTGCC 
CCAAAGTGCT ACATCATTCT TTTACATCCA GAGAGAAACA CCAAAAATGT CATAATGGGA 
AGAGAAACAC AAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001332214.6
CDS	23..2623
Translation	MWFTLYIYICLSLNCISATLILKPSMCLLQRSFKLNGMYQDGDFILGGLFEVHFFTVFPDLTFTTNPKSPYCEIFNMEGFQHAQTMAFAIEEINKNPKLLPNITLGYHLYDNCVMLGMAFRAAISLTSGTEESFLNLNCSGPSPVIGIVGDPSSTPSIAISSVLGLFRVPIVSHYATCSCLSNRKKYPSFFRTIPSDAFQVRAIVQVLKHFKWTWVGLLYSDDDYGVYAAQSFQQEMRQFGLCAAFSEFLPHDNNPRTIQHIMGVIQGSTAKAVVVFAPSSFLIPLMNEVVLQNMTGRQWIASEAWATSLESHIPSFQPFLRGTIGIAIRRGEIQRLHDFLLRIRPSNDPKNYMLRIFWENMFGCSFGKGDTEGEQVIKVCTGQEDLSTTNTPYTDVSGLRAAYNVYKAVYALAHALHDLMECEEGKGPFSKNSCADKTNLKPWQVVHYLQNVNFTTGFGDYVSFDKNGDALAIYDVLNWQPSSDESIRIYTVGVVKEGTETGMVLTLDEDAIFWNFETRKPPRSVCSESCPPGTRRATRKGLPVCCFDCLPCGDGEISNTTNAVECFLCPDEFWSNQYNNHCVPKEVEFLSYEDPLGISLTTASLLGTCFCALVIFVFALHRNTPIVRANNSELSFLLLLSLKLCFLCVLLFIGRPQLWTCQLRHVVFGISFVLCMSSILVKTMVVIAVFKSSRPEGKGAIKWFGAVQQRSTVVVLTVLQVLICAVWLSTASPTPYKNNQYIRSKIVYECAIGSVVGFSMLLSYIGFLAAASFLLAFLARNLPDNFNEAKFITFSMLIFSAVWIAFVPAYVSSPGKYTVAVEIFAILASTFGLLIAIFAPKCYIILLHPERNTKNVIMGRETQNK

Target ORF information:

RefSeq Version	XM_001332178.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001332178.6

ORF Insert Sequence:

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGGTACTCAC ACTGGATGAA GATGCAATAT TCTGGAATTT TGAGACGAGA 
AAACCCCCAC GGTCTGTGTG CAGTGAAAGC TGCCCTCCAG GCACCAGACG AGCCACTCGC 
AAGGGTCTTC CTGTCTGCTG TTTTGACTGC CTGCCATGTG GAGATGGAGA GATTTCTAAC 
ACAACAAATG CTGTTGAGTG CTTTCTATGT CCAGATGAGT TTTGGTCCAA TCAATATAAT 
AATCACTGTG TACCAAAAGA AGTAGAGTTT CTCTCCTATG AAGATCCTCT GGGGATCTCC 
CTGACCACTG CTTCTCTGCT TGGCACCTGC TTCTGTGCTC TTGTGATATT TGTTTTTGCT 
CTTCATCGTA ATACTCCTAT AGTACGTGCA AATAATTCAG AGCTCAGCTT CCTACTGCTG 
CTGTCACTCA AACTGTGTTT CCTTTGTGTC CTGCTGTTTA TTGGTCGACC ACAGCTGTGG 
ACATGTCAGT TAAGACATGT TGTGTTTGGT ATAAGCTTTG TCCTGTGCAT GTCCAGTATT 
CTGGTCAAGA CTATGGTGGT AATAGCTGTG TTCAAGTCCT CTCGGCCAGA AGGCAAAGGA 
GCAATAAAAT GGTTTGGAGC AGTTCAACAG AGAAGCACAG TTGTTGTCCT CACAGTCCTC 
CAGGTTCTTA TATGTGCAGT CTGGTTATCA ACTGCCTCTC CAACACCCTA TAAAAACAAC 
CAATATATCC GTTCTAAAAT AGTATATGAA TGTGCTATAG GTTCAGTTGT TGGGTTTTCA 
ATGTTACTCA GCTATATTGG CTTTTTGGCA GCAGCCAGCT TTTTGTTAGC CTTCCTGGCT 
AGAAATCTTC CAGATAATTT TAATGAAGCA AAGTTCATCA CATTTAGCAT GTTGATTTTT 
AGTGCCGTGT GGATTGCATT TGTTCCAGCG TATGTGAGTT CACCAGGTAA ATACACAGTG 
GCTGTAGAAA TTTTCGCCAT TCTAGCTTCG ACTTTTGGTT TACTGATTGC CATATTTGCC 
CCAAAGTGCT ACATCATTCT TTTACATCCA GAGAGAAACA CCAAAAATGT CATAATGGGA 
AGAGAAACAC AAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-47K20.9 ( XM_001332178.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001332214.6 Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa46705
Clone ID Related Accession (Same CDS sequence)	XM_001332178.6 , XM_001332178.7
Accession Version	XM_001332178.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	extracellular calcium-sensing receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001332178.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGGTACTCAC ACTGGATGAA GATGCAATAT TCTGGAATTT TGAGACGAGA 
AAACCCCCAC GGTCTGTGTG CAGTGAAAGC TGCCCTCCAG GCACCAGACG AGCCACTCGC 
AAGGGTCTTC CTGTCTGCTG TTTTGACTGC CTGCCATGTG GAGATGGAGA GATTTCTAAC 
ACAACAAATG CTGTTGAGTG CTTTCTATGT CCAGATGAGT TTTGGTCCAA TCAATATAAT 
AATCACTGTG TACCAAAAGA AGTAGAGTTT CTCTCCTATG AAGATCCTCT GGGGATCTCC 
CTGACCACTG CTTCTCTGCT TGGCACCTGC TTCTGTGCTC TTGTGATATT TGTTTTTGCT 
CTTCATCGTA ATACTCCTAT AGTACGTGCA AATAATTCAG AGCTCAGCTT CCTACTGCTG 
CTGTCACTCA AACTGTGTTT CCTTTGTGTC CTGCTGTTTA TTGGTCGACC ACAGCTGTGG 
ACATGTCAGT TAAGACATGT TGTGTTTGGT ATAAGCTTTG TCCTGTGCAT GTCCAGTATT 
CTGGTCAAGA CTATGGTGGT AATAGCTGTG TTCAAGTCCT CTCGGCCAGA AGGCAAAGGA 
GCAATAAAAT GGTTTGGAGC AGTTCAACAG AGAAGCACAG TTGTTGTCCT CACAGTCCTC 
CAGGTTCTTA TATGTGCAGT CTGGTTATCA ACTGCCTCTC CAACACCCTA TAAAAACAAC 
CAATATATCC GTTCTAAAAT AGTATATGAA TGTGCTATAG GTTCAGTTGT TGGGTTTTCA 
ATGTTACTCA GCTATATTGG CTTTTTGGCA GCAGCCAGCT TTTTGTTAGC CTTCCTGGCT 
AGAAATCTTC CAGATAATTT TAATGAAGCA AAGTTCATCA CATTTAGCAT GTTGATTTTT 
AGTGCCGTGT GGATTGCATT TGTTCCAGCG TATGTGAGTT CACCAGGTAA ATACACAGTG 
GCTGTAGAAA TTTTCGCCAT TCTAGCTTCG ACTTTTGGTT TACTGATTGC CATATTTGCC 
CCAAAGTGCT ACATCATTCT TTTACATCCA GAGAGAAACA CCAAAAATGT CATAATGGGA 
AGAGAAACAC AAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001332214.6
CDS	24..2624
Translation	MWFTLYIYICLSLNCISATLILKPSMCLLQRSFKLNGMYQDGDFILGGLFEVHFFTVFPDLTFTTNPKSPYCEIFNMEGFQHAQTMAFAIEEINKNPKLLPNITLGYHLYDNCVMLGMAFRAAISLTSGTEESFLNLNCSGPSPVIGIVGDPSSTPSIAISSVLGLFRVPIVSHYATCSCLSNRKKYPSFFRTIPSDAFQVRAIVQVLKHFKWTWVGLLYSDDDYGVYAAQSFQQEMRQFGLCAAFSEFLPHDNNPRTIQHIMGVIQGSTAKAVVVFAPSSFLIPLMNEVVLQNMTGRQWIASEAWATSLESHIPSFQPFLRGTIGIAIRRGEIQRLHDFLLRIRPSNDPKNYMLRIFWENMFGCSFGKGDTEGEQVIKVCTGQEDLSTTNTPYTDVSGLRAAYNVYKAVYALAHALHDLMECEEGKGPFSKNSCADKTNLKPWQVVHYLQNVNFTTGFGDYVSFDKNGDALAIYDVLNWQPSSDESIRIYTVGVVKEGTETGMVLTLDEDAIFWNFETRKPPRSVCSESCPPGTRRATRKGLPVCCFDCLPCGDGEISNTTNAVECFLCPDEFWSNQYNNHCVPKEVEFLSYEDPLGISLTTASLLGTCFCALVIFVFALHRNTPIVRANNSELSFLLLLSLKLCFLCVLLFIGRPQLWTCQLRHVVFGISFVLCMSSILVKTMVVIAVFKSSRPEGKGAIKWFGAVQQRSTVVVLTVLQVLICAVWLSTASPTPYKNNQYIRSKIVYECAIGSVVGFSMLLSYIGFLAAASFLLAFLARNLPDNFNEAKFITFSMLIFSAVWIAFVPAYVSSPGKYTVAVEIFAILASTFGLLIAIFAPKCYIILLHPERNTKNVIMGRETQNK

Target ORF information:

RefSeq Version	XM_001332178.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001332178.7

ORF Insert Sequence:

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGGTACTCAC ACTGGATGAA GATGCAATAT TCTGGAATTT TGAGACGAGA 
AAACCCCCAC GGTCTGTGTG CAGTGAAAGC TGCCCTCCAG GCACCAGACG AGCCACTCGC 
AAGGGTCTTC CTGTCTGCTG TTTTGACTGC CTGCCATGTG GAGATGGAGA GATTTCTAAC 
ACAACAAATG CTGTTGAGTG CTTTCTATGT CCAGATGAGT TTTGGTCCAA TCAATATAAT 
AATCACTGTG TACCAAAAGA AGTAGAGTTT CTCTCCTATG AAGATCCTCT GGGGATCTCC 
CTGACCACTG CTTCTCTGCT TGGCACCTGC TTCTGTGCTC TTGTGATATT TGTTTTTGCT 
CTTCATCGTA ATACTCCTAT AGTACGTGCA AATAATTCAG AGCTCAGCTT CCTACTGCTG 
CTGTCACTCA AACTGTGTTT CCTTTGTGTC CTGCTGTTTA TTGGTCGACC ACAGCTGTGG 
ACATGTCAGT TAAGACATGT TGTGTTTGGT ATAAGCTTTG TCCTGTGCAT GTCCAGTATT 
CTGGTCAAGA CTATGGTGGT AATAGCTGTG TTCAAGTCCT CTCGGCCAGA AGGCAAAGGA 
GCAATAAAAT GGTTTGGAGC AGTTCAACAG AGAAGCACAG TTGTTGTCCT CACAGTCCTC 
CAGGTTCTTA TATGTGCAGT CTGGTTATCA ACTGCCTCTC CAACACCCTA TAAAAACAAC 
CAATATATCC GTTCTAAAAT AGTATATGAA TGTGCTATAG GTTCAGTTGT TGGGTTTTCA 
ATGTTACTCA GCTATATTGG CTTTTTGGCA GCAGCCAGCT TTTTGTTAGC CTTCCTGGCT 
AGAAATCTTC CAGATAATTT TAATGAAGCA AAGTTCATCA CATTTAGCAT GTTGATTTTT 
AGTGCCGTGT GGATTGCATT TGTTCCAGCG TATGTGAGTT CACCAGGTAA ATACACAGTG 
GCTGTAGAAA TTTTCGCCAT TCTAGCTTCG ACTTTTGGTT TACTGATTGC CATATTTGCC 
CCAAAGTGCT ACATCATTCT TTTACATCCA GAGAGAAACA CCAAAAATGT CATAATGGGA 
AGAGAAACAC AAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-47K20.9 ( XM_009293762.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292037.1 Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46706
Clone ID Related Accession (Same CDS sequence)	XM_009293762.2 , XM_009293762.1
Accession Version	XM_009293762.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	extracellular calcium-sensing receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
TCTTTTCAGC AAGAAATGCG GCAATTTGGA CTTTGTGCTG CATTTTCTGA ATTCTTGCCT 
CATGATAATA ATCCCAGAAC CATACAGCAT ATAATGGGGG TTATTCAGGG CTCTACAGCA 
AAAGCGGTGG TTGTTTTTGC CCCATCATCC TTTCTCATAC CTTTGATGAA TGAGGTGGTT 
TTGCAGAATA TGACAGGCAG GCAGTGGATT GCAAGTGAAG CTTGGGCCAC CTCTCTTGAA 
TCCCATATTC CAAGTTTCCA GCCCTTTTTG AGGGGCACAA TAGGAATTGC TATCAGGCGT 
GGAGAGATTC AAAGACTTCA TGACTTTCTA CTACGTATTC GCCCTAGTAA TGATCCAAAA 
AATTATATGT TGAGAATCTT CTGGGAGAAT ATGTTTGGGT GCAGTTTTGG TAAAGGAGAT 
ACTGAAGGAG AGCAAGTCAT AAAGGTATGT ACAGGACAGG AGGATCTGAG CACCACAAAT 
ACACCATACA CTGACGTCTC AGGACTAAGA GCAGCTTATA ATGTATATAA GGCAGTTTAT 
GCACTGGCAC ATGCCCTTCA TGACTTGATG GAGTGTGAGG AGGGGAAAGG ACCATTCAGT 
AAAAACAGCT GTGCTGACAA AACAAACCTA AAACCCTGGC AGGTGGTTCA CTACCTACAG 
AATGTAAACT TCACTACGGG CTTTGGGGAT TATGTGTCAT TTGATAAGAA TGGAGATGCT 
TTGGCTATTT ATGATGTACT AAACTGGCAG CCAAGCTCCG ATGAATCAAT AAGGATCTAC 
ACGGTTGGTG TTGTAAAAGA AGGAACAGAA ACTGGAATGG TACTCACACT GGATGAAGAT 
GCAATATTCT GGAATTTTGA GACGAGAAAA CCCCCACGGT CTGTGTGCAG TGAAAGCTGC 
CCTCCAGGCA CCAGACGAGC CACTCGCAAG GGTCTTCCTG TCTGCTGTTT TGACTGCCTG 
CCATGTGGAG ATGGAGAGAT TTCTAACACA ACAAATGCTG TTGAGTGCTT TCTATGTCCA 
GATGAGTTTT GGTCCAATCA ATATAATAAT CACTGTGTAC CAAAAGAAGT AGAGTTTCTC 
TCCTATGAAG ATCCTCTGGG GATCTCCCTG ACCACTGCTT CTCTGCTTGG CACCTGCTTC 
TGTGCTCTTG TGATATTTGT TTTTGCTCTT CATCGTAATA CTCCTATAGT ACGTGCAAAT 
AATTCAGAGC TCAGCTTCCT ACTGCTGCTG TCACTCAAAC TGTGTTTCCT TTGTGTCCTG 
CTGTTTATTG GTCGACCACA GCTGTGGACA TGTCAGTTAA GACATGTTGT GTTTGGTATA 
AGCTTTGTCC TGTGCATGTC CAGTATTCTG GTCAAGACTA TGGTGGTAAT AGCTGTGTTC 
AAGTCCTCTC GGCCAGAAGG CAAAGGAGCA ATAAAATGGT TTGGAGCAGT TCAACAGAGA 
AGCACAGTTG TTGTCCTCAC AGTCCTCCAG GTTCTTATAT GTGCAGTCTG GTTATCAACT 
GCCTCTCCAA CACCCTATAA AAACAACCAA TATATCCGTT CTAAAATAGT ATATGAATGT 
GCTATAGGTT CAGTTGTTGG GTTTTCAATG TTACTCAGCT ATATTGGCTT TTTGGCAGCA 
GCCAGCTTTT TGTTAGCCTT CCTGGCTAGA AATCTTCCAG ATAATTTTAA TGAAGCAAAG 
TTCATCACAT TTAGCATGTT GATTTTTAGT GCCGTGTGGA TTGCATTTGT TCCAGCGTAT 
GTGAGTTCAC CAGGTAAATA CACAGTGGCT GTAGAAATTT TCGCCATTCT AGCTTCGACT 
TTTGGTTTAC TGATTGCCAT ATTTGCCCCA AAGTGCTACA TCATTCTTTT ACATCCAGAG 
AGAAACACCA AAAATGTCAT AATGGGAAGA GAAACACAAA ATAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292037.1
CDS	24..2531
Translation	MWFTLYIYICLSLNCISATLILKPSMCLLQRSFKLNGMYQDGDFILGGLFEVHFFTVFPDLTFTTNPKSPYCEIFNMEGFQHAQTMAFAIEEINKNPKLLPNITLGYHLYDNCVMLGMAFRAAISLTSGTEESFLNLNCSGPSPVIGIVGDPSSTPSIAISSVLGLFRVPIVSHYATCSCLSNRKKYPSFFRTIPSDAFQSFQQEMRQFGLCAAFSEFLPHDNNPRTIQHIMGVIQGSTAKAVVVFAPSSFLIPLMNEVVLQNMTGRQWIASEAWATSLESHIPSFQPFLRGTIGIAIRRGEIQRLHDFLLRIRPSNDPKNYMLRIFWENMFGCSFGKGDTEGEQVIKVCTGQEDLSTTNTPYTDVSGLRAAYNVYKAVYALAHALHDLMECEEGKGPFSKNSCADKTNLKPWQVVHYLQNVNFTTGFGDYVSFDKNGDALAIYDVLNWQPSSDESIRIYTVGVVKEGTETGMVLTLDEDAIFWNFETRKPPRSVCSESCPPGTRRATRKGLPVCCFDCLPCGDGEISNTTNAVECFLCPDEFWSNQYNNHCVPKEVEFLSYEDPLGISLTTASLLGTCFCALVIFVFALHRNTPIVRANNSELSFLLLLSLKLCFLCVLLFIGRPQLWTCQLRHVVFGISFVLCMSSILVKTMVVIAVFKSSRPEGKGAIKWFGAVQQRSTVVVLTVLQVLICAVWLSTASPTPYKNNQYIRSKIVYECAIGSVVGFSMLLSYIGFLAAASFLLAFLARNLPDNFNEAKFITFSMLIFSAVWIAFVPAYVSSPGKYTVAVEIFAILASTFGLLIAIFAPKCYIILLHPERNTKNVIMGRETQNK

Target ORF information:

RefSeq Version	XM_009293762.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293762.2

ORF Insert Sequence:

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
TCTTTTCAGC AAGAAATGCG GCAATTTGGA CTTTGTGCTG CATTTTCTGA ATTCTTGCCT 
CATGATAATA ATCCCAGAAC CATACAGCAT ATAATGGGGG TTATTCAGGG CTCTACAGCA 
AAAGCGGTGG TTGTTTTTGC CCCATCATCC TTTCTCATAC CTTTGATGAA TGAGGTGGTT 
TTGCAGAATA TGACAGGCAG GCAGTGGATT GCAAGTGAAG CTTGGGCCAC CTCTCTTGAA 
TCCCATATTC CAAGTTTCCA GCCCTTTTTG AGGGGCACAA TAGGAATTGC TATCAGGCGT 
GGAGAGATTC AAAGACTTCA TGACTTTCTA CTACGTATTC GCCCTAGTAA TGATCCAAAA 
AATTATATGT TGAGAATCTT CTGGGAGAAT ATGTTTGGGT GCAGTTTTGG TAAAGGAGAT 
ACTGAAGGAG AGCAAGTCAT AAAGGTATGT ACAGGACAGG AGGATCTGAG CACCACAAAT 
ACACCATACA CTGACGTCTC AGGACTAAGA GCAGCTTATA ATGTATATAA GGCAGTTTAT 
GCACTGGCAC ATGCCCTTCA TGACTTGATG GAGTGTGAGG AGGGGAAAGG ACCATTCAGT 
AAAAACAGCT GTGCTGACAA AACAAACCTA AAACCCTGGC AGGTGGTTCA CTACCTACAG 
AATGTAAACT TCACTACGGG CTTTGGGGAT TATGTGTCAT TTGATAAGAA TGGAGATGCT 
TTGGCTATTT ATGATGTACT AAACTGGCAG CCAAGCTCCG ATGAATCAAT AAGGATCTAC 
ACGGTTGGTG TTGTAAAAGA AGGAACAGAA ACTGGAATGG TACTCACACT GGATGAAGAT 
GCAATATTCT GGAATTTTGA GACGAGAAAA CCCCCACGGT CTGTGTGCAG TGAAAGCTGC 
CCTCCAGGCA CCAGACGAGC CACTCGCAAG GGTCTTCCTG TCTGCTGTTT TGACTGCCTG 
CCATGTGGAG ATGGAGAGAT TTCTAACACA ACAAATGCTG TTGAGTGCTT TCTATGTCCA 
GATGAGTTTT GGTCCAATCA ATATAATAAT CACTGTGTAC CAAAAGAAGT AGAGTTTCTC 
TCCTATGAAG ATCCTCTGGG GATCTCCCTG ACCACTGCTT CTCTGCTTGG CACCTGCTTC 
TGTGCTCTTG TGATATTTGT TTTTGCTCTT CATCGTAATA CTCCTATAGT ACGTGCAAAT 
AATTCAGAGC TCAGCTTCCT ACTGCTGCTG TCACTCAAAC TGTGTTTCCT TTGTGTCCTG 
CTGTTTATTG GTCGACCACA GCTGTGGACA TGTCAGTTAA GACATGTTGT GTTTGGTATA 
AGCTTTGTCC TGTGCATGTC CAGTATTCTG GTCAAGACTA TGGTGGTAAT AGCTGTGTTC 
AAGTCCTCTC GGCCAGAAGG CAAAGGAGCA ATAAAATGGT TTGGAGCAGT TCAACAGAGA 
AGCACAGTTG TTGTCCTCAC AGTCCTCCAG GTTCTTATAT GTGCAGTCTG GTTATCAACT 
GCCTCTCCAA CACCCTATAA AAACAACCAA TATATCCGTT CTAAAATAGT ATATGAATGT 
GCTATAGGTT CAGTTGTTGG GTTTTCAATG TTACTCAGCT ATATTGGCTT TTTGGCAGCA 
GCCAGCTTTT TGTTAGCCTT CCTGGCTAGA AATCTTCCAG ATAATTTTAA TGAAGCAAAG 
TTCATCACAT TTAGCATGTT GATTTTTAGT GCCGTGTGGA TTGCATTTGT TCCAGCGTAT 
GTGAGTTCAC CAGGTAAATA CACAGTGGCT GTAGAAATTT TCGCCATTCT AGCTTCGACT 
TTTGGTTTAC TGATTGCCAT ATTTGCCCCA AAGTGCTACA TCATTCTTTT ACATCCAGAG 
AGAAACACCA AAAATGTCAT AATGGGAAGA GAAACACAAA ATAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-47K20.9 ( XM_009293762.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292037.1 Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46706
Clone ID Related Accession (Same CDS sequence)	XM_009293762.2 , XM_009293762.1
Accession Version	XM_009293762.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	extracellular calcium-sensing receptor isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
TCTTTTCAGC AAGAAATGCG GCAATTTGGA CTTTGTGCTG CATTTTCTGA ATTCTTGCCT 
CATGATAATA ATCCCAGAAC CATACAGCAT ATAATGGGGG TTATTCAGGG CTCTACAGCA 
AAAGCGGTGG TTGTTTTTGC CCCATCATCC TTTCTCATAC CTTTGATGAA TGAGGTGGTT 
TTGCAGAATA TGACAGGCAG GCAGTGGATT GCAAGTGAAG CTTGGGCCAC CTCTCTTGAA 
TCCCATATTC CAAGTTTCCA GCCCTTTTTG AGGGGCACAA TAGGAATTGC TATCAGGCGT 
GGAGAGATTC AAAGACTTCA TGACTTTCTA CTACGTATTC GCCCTAGTAA TGATCCAAAA 
AATTATATGT TGAGAATCTT CTGGGAGAAT ATGTTTGGGT GCAGTTTTGG TAAAGGAGAT 
ACTGAAGGAG AGCAAGTCAT AAAGGTATGT ACAGGACAGG AGGATCTGAG CACCACAAAT 
ACACCATACA CTGACGTCTC AGGACTAAGA GCAGCTTATA ATGTATATAA GGCAGTTTAT 
GCACTGGCAC ATGCCCTTCA TGACTTGATG GAGTGTGAGG AGGGGAAAGG ACCATTCAGT 
AAAAACAGCT GTGCTGACAA AACAAACCTA AAACCCTGGC AGGTGGTTCA CTACCTACAG 
AATGTAAACT TCACTACGGG CTTTGGGGAT TATGTGTCAT TTGATAAGAA TGGAGATGCT 
TTGGCTATTT ATGATGTACT AAACTGGCAG CCAAGCTCCG ATGAATCAAT AAGGATCTAC 
ACGGTTGGTG TTGTAAAAGA AGGAACAGAA ACTGGAATGG TACTCACACT GGATGAAGAT 
GCAATATTCT GGAATTTTGA GACGAGAAAA CCCCCACGGT CTGTGTGCAG TGAAAGCTGC 
CCTCCAGGCA CCAGACGAGC CACTCGCAAG GGTCTTCCTG TCTGCTGTTT TGACTGCCTG 
CCATGTGGAG ATGGAGAGAT TTCTAACACA ACAAATGCTG TTGAGTGCTT TCTATGTCCA 
GATGAGTTTT GGTCCAATCA ATATAATAAT CACTGTGTAC CAAAAGAAGT AGAGTTTCTC 
TCCTATGAAG ATCCTCTGGG GATCTCCCTG ACCACTGCTT CTCTGCTTGG CACCTGCTTC 
TGTGCTCTTG TGATATTTGT TTTTGCTCTT CATCGTAATA CTCCTATAGT ACGTGCAAAT 
AATTCAGAGC TCAGCTTCCT ACTGCTGCTG TCACTCAAAC TGTGTTTCCT TTGTGTCCTG 
CTGTTTATTG GTCGACCACA GCTGTGGACA TGTCAGTTAA GACATGTTGT GTTTGGTATA 
AGCTTTGTCC TGTGCATGTC CAGTATTCTG GTCAAGACTA TGGTGGTAAT AGCTGTGTTC 
AAGTCCTCTC GGCCAGAAGG CAAAGGAGCA ATAAAATGGT TTGGAGCAGT TCAACAGAGA 
AGCACAGTTG TTGTCCTCAC AGTCCTCCAG GTTCTTATAT GTGCAGTCTG GTTATCAACT 
GCCTCTCCAA CACCCTATAA AAACAACCAA TATATCCGTT CTAAAATAGT ATATGAATGT 
GCTATAGGTT CAGTTGTTGG GTTTTCAATG TTACTCAGCT ATATTGGCTT TTTGGCAGCA 
GCCAGCTTTT TGTTAGCCTT CCTGGCTAGA AATCTTCCAG ATAATTTTAA TGAAGCAAAG 
TTCATCACAT TTAGCATGTT GATTTTTAGT GCCGTGTGGA TTGCATTTGT TCCAGCGTAT 
GTGAGTTCAC CAGGTAAATA CACAGTGGCT GTAGAAATTT TCGCCATTCT AGCTTCGACT 
TTTGGTTTAC TGATTGCCAT ATTTGCCCCA AAGTGCTACA TCATTCTTTT ACATCCAGAG 
AGAAACACCA AAAATGTCAT AATGGGAAGA GAAACACAAA ATAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292037.1
CDS	23..2530
Translation	MWFTLYIYICLSLNCISATLILKPSMCLLQRSFKLNGMYQDGDFILGGLFEVHFFTVFPDLTFTTNPKSPYCEIFNMEGFQHAQTMAFAIEEINKNPKLLPNITLGYHLYDNCVMLGMAFRAAISLTSGTEESFLNLNCSGPSPVIGIVGDPSSTPSIAISSVLGLFRVPIVSHYATCSCLSNRKKYPSFFRTIPSDAFQSFQQEMRQFGLCAAFSEFLPHDNNPRTIQHIMGVIQGSTAKAVVVFAPSSFLIPLMNEVVLQNMTGRQWIASEAWATSLESHIPSFQPFLRGTIGIAIRRGEIQRLHDFLLRIRPSNDPKNYMLRIFWENMFGCSFGKGDTEGEQVIKVCTGQEDLSTTNTPYTDVSGLRAAYNVYKAVYALAHALHDLMECEEGKGPFSKNSCADKTNLKPWQVVHYLQNVNFTTGFGDYVSFDKNGDALAIYDVLNWQPSSDESIRIYTVGVVKEGTETGMVLTLDEDAIFWNFETRKPPRSVCSESCPPGTRRATRKGLPVCCFDCLPCGDGEISNTTNAVECFLCPDEFWSNQYNNHCVPKEVEFLSYEDPLGISLTTASLLGTCFCALVIFVFALHRNTPIVRANNSELSFLLLLSLKLCFLCVLLFIGRPQLWTCQLRHVVFGISFVLCMSSILVKTMVVIAVFKSSRPEGKGAIKWFGAVQQRSTVVVLTVLQVLICAVWLSTASPTPYKNNQYIRSKIVYECAIGSVVGFSMLLSYIGFLAAASFLLAFLARNLPDNFNEAKFITFSMLIFSAVWIAFVPAYVSSPGKYTVAVEIFAILASTFGLLIAIFAPKCYIILLHPERNTKNVIMGRETQNK

Target ORF information:

RefSeq Version	XM_009293762.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293762.1

ORF Insert Sequence:

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
TCTTTTCAGC AAGAAATGCG GCAATTTGGA CTTTGTGCTG CATTTTCTGA ATTCTTGCCT 
CATGATAATA ATCCCAGAAC CATACAGCAT ATAATGGGGG TTATTCAGGG CTCTACAGCA 
AAAGCGGTGG TTGTTTTTGC CCCATCATCC TTTCTCATAC CTTTGATGAA TGAGGTGGTT 
TTGCAGAATA TGACAGGCAG GCAGTGGATT GCAAGTGAAG CTTGGGCCAC CTCTCTTGAA 
TCCCATATTC CAAGTTTCCA GCCCTTTTTG AGGGGCACAA TAGGAATTGC TATCAGGCGT 
GGAGAGATTC AAAGACTTCA TGACTTTCTA CTACGTATTC GCCCTAGTAA TGATCCAAAA 
AATTATATGT TGAGAATCTT CTGGGAGAAT ATGTTTGGGT GCAGTTTTGG TAAAGGAGAT 
ACTGAAGGAG AGCAAGTCAT AAAGGTATGT ACAGGACAGG AGGATCTGAG CACCACAAAT 
ACACCATACA CTGACGTCTC AGGACTAAGA GCAGCTTATA ATGTATATAA GGCAGTTTAT 
GCACTGGCAC ATGCCCTTCA TGACTTGATG GAGTGTGAGG AGGGGAAAGG ACCATTCAGT 
AAAAACAGCT GTGCTGACAA AACAAACCTA AAACCCTGGC AGGTGGTTCA CTACCTACAG 
AATGTAAACT TCACTACGGG CTTTGGGGAT TATGTGTCAT TTGATAAGAA TGGAGATGCT 
TTGGCTATTT ATGATGTACT AAACTGGCAG CCAAGCTCCG ATGAATCAAT AAGGATCTAC 
ACGGTTGGTG TTGTAAAAGA AGGAACAGAA ACTGGAATGG TACTCACACT GGATGAAGAT 
GCAATATTCT GGAATTTTGA GACGAGAAAA CCCCCACGGT CTGTGTGCAG TGAAAGCTGC 
CCTCCAGGCA CCAGACGAGC CACTCGCAAG GGTCTTCCTG TCTGCTGTTT TGACTGCCTG 
CCATGTGGAG ATGGAGAGAT TTCTAACACA ACAAATGCTG TTGAGTGCTT TCTATGTCCA 
GATGAGTTTT GGTCCAATCA ATATAATAAT CACTGTGTAC CAAAAGAAGT AGAGTTTCTC 
TCCTATGAAG ATCCTCTGGG GATCTCCCTG ACCACTGCTT CTCTGCTTGG CACCTGCTTC 
TGTGCTCTTG TGATATTTGT TTTTGCTCTT CATCGTAATA CTCCTATAGT ACGTGCAAAT 
AATTCAGAGC TCAGCTTCCT ACTGCTGCTG TCACTCAAAC TGTGTTTCCT TTGTGTCCTG 
CTGTTTATTG GTCGACCACA GCTGTGGACA TGTCAGTTAA GACATGTTGT GTTTGGTATA 
AGCTTTGTCC TGTGCATGTC CAGTATTCTG GTCAAGACTA TGGTGGTAAT AGCTGTGTTC 
AAGTCCTCTC GGCCAGAAGG CAAAGGAGCA ATAAAATGGT TTGGAGCAGT TCAACAGAGA 
AGCACAGTTG TTGTCCTCAC AGTCCTCCAG GTTCTTATAT GTGCAGTCTG GTTATCAACT 
GCCTCTCCAA CACCCTATAA AAACAACCAA TATATCCGTT CTAAAATAGT ATATGAATGT 
GCTATAGGTT CAGTTGTTGG GTTTTCAATG TTACTCAGCT ATATTGGCTT TTTGGCAGCA 
GCCAGCTTTT TGTTAGCCTT CCTGGCTAGA AATCTTCCAG ATAATTTTAA TGAAGCAAAG 
TTCATCACAT TTAGCATGTT GATTTTTAGT GCCGTGTGGA TTGCATTTGT TCCAGCGTAT 
GTGAGTTCAC CAGGTAAATA CACAGTGGCT GTAGAAATTT TCGCCATTCT AGCTTCGACT 
TTTGGTTTAC TGATTGCCAT ATTTGCCCCA AAGTGCTACA TCATTCTTTT ACATCCAGAG 
AGAAACACCA AAAATGTCAT AATGGGAAGA GAAACACAAA ATAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-47K20.9 ( XM_009293764.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292039.1 Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X3, mRNA.

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CloneID	ODa46708
Clone ID Related Accession (Same CDS sequence)	XM_009293764.1
Accession Version	XM_009293764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	extracellular calcium-sensing receptor isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGACAAATGA TTTGAAAATG ATGGCCAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292039.1
CDS	23..1564
Translation	MWFTLYIYICLSLNCISATLILKPSMCLLQRSFKLNGMYQDGDFILGGLFEVHFFTVFPDLTFTTNPKSPYCEIFNMEGFQHAQTMAFAIEEINKNPKLLPNITLGYHLYDNCVMLGMAFRAAISLTSGTEESFLNLNCSGPSPVIGIVGDPSSTPSIAISSVLGLFRVPIVSHYATCSCLSNRKKYPSFFRTIPSDAFQVRAIVQVLKHFKWTWVGLLYSDDDYGVYAAQSFQQEMRQFGLCAAFSEFLPHDNNPRTIQHIMGVIQGSTAKAVVVFAPSSFLIPLMNEVVLQNMTGRQWIASEAWATSLESHIPSFQPFLRGTIGIAIRRGEIQRLHDFLLRIRPSNDPKNYMLRIFWENMFGCSFGKGDTEGEQVIKVCTGQEDLSTTNTPYTDVSGLRAAYNVYKAVYALAHALHDLMECEEGKGPFSKNSCADKTNLKPWQVVHYLQNVNFTTGFGDYVSFDKNGDALAIYDVLNWQPSSDESIRIYTVGVVKEGTETGMTNDLKMMAR

Target ORF information:

RefSeq Version	XM_009293764.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293764.1

ORF Insert Sequence:

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGACAAATGA TTTGAAAATG ATGGCCAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-47K20.9 ( XM_009293765.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292040.1 Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa46709
Clone ID Related Accession (Same CDS sequence)	XM_009293765.1
Accession Version	XM_009293765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	extracellular calcium-sensing receptor isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292040.1
CDS	23..1540
Translation	MWFTLYIYICLSLNCISATLILKPSMCLLQRSFKLNGMYQDGDFILGGLFEVHFFTVFPDLTFTTNPKSPYCEIFNMEGFQHAQTMAFAIEEINKNPKLLPNITLGYHLYDNCVMLGMAFRAAISLTSGTEESFLNLNCSGPSPVIGIVGDPSSTPSIAISSVLGLFRVPIVSHYATCSCLSNRKKYPSFFRTIPSDAFQVRAIVQVLKHFKWTWVGLLYSDDDYGVYAAQSFQQEMRQFGLCAAFSEFLPHDNNPRTIQHIMGVIQGSTAKAVVVFAPSSFLIPLMNEVVLQNMTGRQWIASEAWATSLESHIPSFQPFLRGTIGIAIRRGEIQRLHDFLLRIRPSNDPKNYMLRIFWENMFGCSFGKGDTEGEQVIKVCTGQEDLSTTNTPYTDVSGLRAAYNVYKAVYALAHALHDLMECEEGKGPFSKNSCADKTNLKPWQVVHYLQNVNFTTGFGDYVSFDKNGDALAIYDVLNWQPSSDESIRIYTVGVVKEGTETGMI

Target ORF information:

RefSeq Version	XM_009293765.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-47k20.9 (si:dkey-47k20.9), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293765.1

ORF Insert Sequence:

ATGTGGTTTA CTCTGTATAT TTACATTTGC CTGTCTTTGA ATTGTATCTC TGCAACTTTA 
ATCCTCAAAC CAAGTATGTG TTTGCTACAA AGGAGTTTTA AGTTGAATGG AATGTACCAG 
GATGGAGATT TTATACTCGG AGGCCTATTT GAGGTTCACT TCTTTACAGT GTTTCCAGAT 
CTGACTTTCA CAACAAATCC AAAATCACCA TACTGTGAAA TATTTAATAT GGAAGGCTTC 
CAGCATGCAC AGACCATGGC TTTTGCAATA GAAGAGATAA ATAAGAATCC AAAACTGCTG 
CCAAACATCA CTCTTGGATA TCATCTTTAT GACAATTGTG TGATGCTAGG AATGGCATTC 
CGGGCTGCCA TTTCACTTAC TAGTGGGACA GAGGAGTCCT TCTTGAACCT AAATTGCAGT 
GGTCCATCAC CAGTGATTGG GATTGTTGGA GATCCAAGTT CAACTCCTTC CATTGCAATT 
TCCAGTGTTC TGGGGCTGTT TCGAGTGCCT ATAGTTAGTC ACTACGCCAC CTGCTCCTGT 
TTGAGTAACA GGAAAAAGTA CCCCTCTTTC TTTAGAACAA TCCCCAGTGA TGCATTTCAG 
GTACGGGCTA TAGTCCAAGT CTTAAAACAT TTTAAATGGA CCTGGGTTGG TCTCCTCTAC 
AGTGATGATG ACTATGGCGT CTATGCTGCT CAGTCTTTTC AGCAAGAAAT GCGGCAATTT 
GGACTTTGTG CTGCATTTTC TGAATTCTTG CCTCATGATA ATAATCCCAG AACCATACAG 
CATATAATGG GGGTTATTCA GGGCTCTACA GCAAAAGCGG TGGTTGTTTT TGCCCCATCA 
TCCTTTCTCA TACCTTTGAT GAATGAGGTG GTTTTGCAGA ATATGACAGG CAGGCAGTGG 
ATTGCAAGTG AAGCTTGGGC CACCTCTCTT GAATCCCATA TTCCAAGTTT CCAGCCCTTT 
TTGAGGGGCA CAATAGGAAT TGCTATCAGG CGTGGAGAGA TTCAAAGACT TCATGACTTT 
CTACTACGTA TTCGCCCTAG TAATGATCCA AAAAATTATA TGTTGAGAAT CTTCTGGGAG 
AATATGTTTG GGTGCAGTTT TGGTAAAGGA GATACTGAAG GAGAGCAAGT CATAAAGGTA 
TGTACAGGAC AGGAGGATCT GAGCACCACA AATACACCAT ACACTGACGT CTCAGGACTA 
AGAGCAGCTT ATAATGTATA TAAGGCAGTT TATGCACTGG CACATGCCCT TCATGACTTG 
ATGGAGTGTG AGGAGGGGAA AGGACCATTC AGTAAAAACA GCTGTGCTGA CAAAACAAAC 
CTAAAACCCT GGCAGGTGGT TCACTACCTA CAGAATGTAA ACTTCACTAC GGGCTTTGGG 
GATTATGTGT CATTTGATAA GAATGGAGAT GCTTTGGCTA TTTATGATGT ACTAAACTGG 
CAGCCAAGCT CCGATGAATC AATAAGGATC TACACGGTTG GTGTTGTAAA AGAAGGAACA 
GAAACTGGAA TGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SI:DKEY-47K20.9 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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