atg12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	atg12
Entrez Gene ID	570965
Full Name	ATG12 autophagy related 12 homolog (S. cerevisiae)
General protein information	Preferred Names ATG12 autophagy related 12 homolog (S. cerevisiae) Names ubiquitin-like protein ATG12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001246200.1	NP_001233129.1	ubiquitin-like protein ATG12

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007296	XP_308525.2
Bos taurus (cattle)	ATG12	NP_001070450.1
Rattus norvegicus (Norway rat)	Atg12	NP_001033584.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012847	XP_307342.3
Homo sapiens (human)	ATG12	NP_004698.3
Pan troglodytes (chimpanzee)	ATG12	NP_001238927.1
Canis lupus familiaris (dog)	ATG12	XP_531866.2
Drosophila melanogaster (fruit fly)	Atg12	NP_648551.3
Xenopus tropicalis (tropical clawed frog)	LOC100493403	XP_002940007.1
Gallus gallus (chicken)	ATG12	XP_424963.1
Danio rerio (zebrafish)	atg12	NP_001233129.1
Mus musculus (house mouse)	Atg12	NP_080493.2

Gene Ontology
Bibliography

Related articles in PubMed

Hepatotoxicity Induced by Isoniazid-Lipopolysaccharide through Endoplasmic Reticulum Stress, Autophagy, and Apoptosis Pathways in Zebrafish.
Zhang Y, Cen J, Jia Z, Hsiao CD, Xia Q, Wang X, Chen X, Wang R, Jiang Z, Zhang L, Liu K
Antimicrobial agents and chemotherapy63(5)(2019 05)

Fasting enhances cold resistance in fish through stimulating lipid catabolism and autophagy.
Lu DL, Ma Q, Wang J, Li LY, Han SL, Limbu SM, Li DL, Chen LQ, Zhang ML, Du ZY
The Journal of physiology597(6)1585-1603(2019 Mar)

Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish.
Chen B, Zheng YM, Zhang JP
Frontiers in endocrinology9366(2018)

Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway.
Zhang J, Zhang X, Liu Y, Su Z, Dawar FU, Dan H, He Y, Gui JF, Mei J
Oncotarget8(67)111807-111818(2017 Dec)

Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes.
Biga PR, Latimer MN, Froehlich JM, Gabillard JC, Seiliez I
Biology open6(11)1720-1725(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following atg12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atg12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35580	NM_001246200.1 Latest version!	Danio rerio ATG12 autophagy related 12 homolog (S. cerevisiae) (atg12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atg12 ( NM_001246200.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001233129.1 Danio rerio ATG12 autophagy related 12 homolog (S. cerevisiae) (atg12), mRNA.

Price & Availability↑

CloneID	ODa35580
Clone ID Related Accession (Same CDS sequence)	NM_001246200.1
Accession Version	NM_001246200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Danio rerio(zebrafish)
Product	ubiquitin-like protein ATG12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HQ450379.1. On Oct 10, 2011 this sequence version replaced XM_694510.4. ##Evidence-Data-START## Transcript exon combination :: HQ450379.1, CO350297.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGACA ACGCAGAATC TCCTACAGAA AACCCGAAAG ATGAACATTC GCTTCAGCAC 
GCTGTCACAG ATCACTCTGA GTCATCGGAC GAGAAGAAAA AGATTGATGT GCTTTTAAAG 
GCGGTCGGTG TCACACCCAT CATGAAGACC AAAAAATGGT CTGTGGAGAG AAGGAGGACG 
ATACAGTCAC TCGCCCAGTT CATCTCACGC TTCCTCAAGC TGGAGCCCAG CGAACAGTTG 
TTTATTTATG TTAATCAGTC GTTTGCGCCA TCACCGGACC AAGAAGTTGG TGTGCTTTTT 
GAGTGTTTCG GAAGTGACGG CAAACTAGTT CTTCATTACT GCAAGTCTCA GGCCTGGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001233129.1
CDS	1..363
Translation	MSDNAESPTENPKDEHSLQHAVTDHSESSDEKKKIDVLLKAVGVTPIMKTKKWSVERRRTIQSLAQFISRFLKLEPSEQLFIYVNQSFAPSPDQEVGVLFECFGSDGKLVLHYCKSQAWG

Target ORF information:

RefSeq Version	NM_001246200.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATG12 autophagy related 12 homolog (S. cerevisiae) (atg12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001246200.1

ORF Insert Sequence:

ATGTCTGACA ACGCAGAATC TCCTACAGAA AACCCGAAAG ATGAACATTC GCTTCAGCAC 
GCTGTCACAG ATCACTCTGA GTCATCGGAC GAGAAGAAAA AGATTGATGT GCTTTTAAAG 
GCGGTCGGTG TCACACCCAT CATGAAGACC AAAAAATGGT CTGTGGAGAG AAGGAGGACG 
ATACAGTCAC TCGCCCAGTT CATCTCACGC TTCCTCAAGC TGGAGCCCAG CGAACAGTTG 
TTTATTTATG TTAATCAGTC GTTTGCGCCA TCACCGGACC AAGAAGTTGG TGTGCTTTTT 
GAGTGTTTCG GAAGTGACGG CAAACTAGTT CTTCATTACT GCAAGTCTCA GGCCTGGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatotoxicity Induced by Isoniazid-Lipopolysaccharide through Endoplasmic Reticulum Stress, Autophagy, and Apoptosis Pathways in Zebrafish.
Antimicrobial agents and chemotherapy63(5)(2019 05)
Zhang Y,Cen J,Jia Z,Hsiao CD,Xia Q,Wang X,Chen X,Wang R,Jiang Z,Zhang L,Liu K

Fasting enhances cold resistance in fish through stimulating lipid catabolism and autophagy.
The Journal of physiology597(6)1585-1603(2019 Mar)
Lu DL,Ma Q,Wang J,Li LY,Han SL,Limbu SM,Li DL,Chen LQ,Zhang ML,Du ZY

Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish.
Frontiers in endocrinology9366(2018)
Chen B,Zheng YM,Zhang JP

Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway.
Oncotarget8(67)111807-111818(2017 Dec)
Zhang J,Zhang X,Liu Y,Su Z,Dawar FU,Dan H,He Y,Gui JF,Mei J

Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes.
Biology open6(11)1720-1725(2017 Nov)
Biga PR,Latimer MN,Froehlich JM,Gabillard JC,Seiliez I