pfkfb2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pfkfb2b
Entrez Gene ID	564852
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b
Synonyms	fj21f03,wu:fj21f03
General protein information	Preferred Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b Names 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155842.3	XP_005155899.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X1
XM_005155843.3	XP_005155900.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X2
XM_021479801.1	XP_021335476.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X4
XM_005155842.4	XP_005155899.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X1
XM_005155843.4	XP_005155900.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X2
XM_021479800.1	XP_021335475.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X3

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06524	XP_003717027.1
Oryza sativa (rice)	Os03g0294200	NP_001049815.1
Mus musculus (house mouse)	Pfkfb2	NP_032851.2
Kluyveromyces lactis	KLLA0B07513g	XP_451863.1
Homo sapiens (human)	PFKFB2	NP_006203.2
Danio rerio (zebrafish)	pfkfb2a	NP_957302.1
Neurospora crassa	NCU09347	XP_958926.1
Schizosaccharomyces pombe (fission yeast)	SPAPB17E12.14c	NP_001018229.2
Pan troglodytes (chimpanzee)	PFKFB2	XP_001165997.2
Macaca mulatta (Rhesus monkey)	PFKFB2	XP_001082732.1
Eremothecium gossypii	AGOS_AEL106W	NP_984755.2
Gallus gallus (chicken)	PFKFB2	XP_004935023.1
Canis lupus familiaris (dog)	PFKFB2	XP_005622364.1
Bos taurus (cattle)	PFKFB2	NP_777237.3
Rattus norvegicus (Norway rat)	Pfkfb2	NP_001029136.1
Danio rerio (zebrafish)	LOC564852	XP_001919792.3
Saccharomyces cerevisiae (baker's yeast)	FBP26	NP_012380.1
Xenopus tropicalis (tropical clawed frog)	pfkfb2	NP_001123395.1

Gene Ontology

The following pfkfb2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfkfb2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa147985	XM_021479801.1 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa147984	XM_021479800.1 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa16998	XM_005155842.4 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa16999	XM_005155843.4 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa16998	XM_005155842.3	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa16999	XM_005155843.3	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pfkfb2b ( XM_021479801.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335476.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa147985
Clone ID Related Accession (Same CDS sequence)	XM_021479801.1
Accession Version	XM_021479801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGTCTC GTGTGGTTGG CAGCCATCTG 
ACTCCGTGTG TGAAGGAATC AACTTTGACT GCTCAGACGA AGTTAATGAC TGTTTCCGCT 
TCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335476.1
CDS	217..1665
Translation	MSAWLQQEEAGPTSSAEAKKSEPRGQEKKCSWASYMTNSPTVIVMIGLPARGKTYMSKKLTRYLNWIGVPTKVFNLGVYRREAVKAYKSYDFFRHDNEEAMKIRKQCALVALQDVKIYLNEEGGQIAVFDATNTTRERRDLILDFAQENAYKVFFVESVCDDPEVIAANILEVKVSSPDYPEAHRERVMDDFLKRIECYKVTYQPLDPDEYDKDLSFIKVINVGRRFLVNRVQDYIQSKIVYYLMNIHVHSHSIYLCRHGESEHNIQGRIGGDSELSSRGRQFASALHEFVEEHKLSNLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGVCEEMTYEMIQNTFPEEFALRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSADDLAYLLCPLHTVMKLTPVAYGCKVEMFHLNVEAVNTHRDRPLSRVVGSHLTPCVKESTLTAQTKLMTVSASE

Target ORF information:

RefSeq Version	XM_021479801.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479801.1

ORF Insert Sequence:

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGTCTC GTGTGGTTGG CAGCCATCTG 
ACTCCGTGTG TGAAGGAATC AACTTTGACT GCTCAGACGA AGTTAATGAC TGTTTCCGCT 
TCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2b ( XM_021479800.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335475.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa147984
Clone ID Related Accession (Same CDS sequence)	XM_021479800.1
Accession Version	XM_021479800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAAGACTCC 
GTGTGTGAAG GAATCAACTT TGACTGCTCA GACGAAGTTA ATGACTGTTT CCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335475.1
CDS	217..1836
Translation	MSAWLQQEEAGPTSSAEAKKSEPRGQEKKCSWASYMTNSPTVIVMIGLPARGKTYMSKKLTRYLNWIGVPTKVFNLGVYRREAVKAYKSYDFFRHDNEEAMKIRKQCALVALQDVKIYLNEEGGQIAVFDATNTTRERRDLILDFAQENAYKVFFVESVCDDPEVIAANILEVKVSSPDYPEAHRERVMDDFLKRIECYKVTYQPLDPDEYDKDLSFIKVINVGRRFLVNRVQDYIQSKIVYYLMNIHVHSHSIYLCRHGESEHNIQGRIGGDSELSSRGRQFASALHEFVEEHKLSNLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGVCEEMTYEMIQNTFPEEFALRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSADDLAYLLCPLHTVMKLTPVAYGCKVEMFHLNVEAVNTHRDRPLQGKVRRDCAPLLLRRNSYTPLSSHDQVKRPRLYSAGNRPWLPLTPSPTTLHFPNTPDGALLLQQSQDSVCEGINFDCSDEVNDCFRF

Target ORF information:

RefSeq Version	XM_021479800.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479800.1

ORF Insert Sequence:

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAAGACTCC 
GTGTGTGAAG GAATCAACTT TGACTGCTCA GACGAAGTTA ATGACTGTTT CCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2b ( XM_005155842.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155899.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16998
Clone ID Related Accession (Same CDS sequence)	XM_005155842.3 , XM_005155842.4
Accession Version	XM_005155842.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAAGTTAGT 
GACAGTTTAA ACAAGCTCCG TAATCTTACA GGGACTCAGT GTTGTCCTGA CACTAACCCA 
CCCTCACACT GGTGTTCTGA ATCACCCCGG AACGTCACAC TTAGTACTGA TCGTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155899.1
CDS	214..1893
Translation	MSAWLQQEEAGPTSSAEAKKSEPRGQEKKCSWASYMTNSPTVIVMIGLPARGKTYMSKKLTRYLNWIGVPTKVFNLGVYRREAVKAYKSYDFFRHDNEEAMKIRKQCALVALQDVKIYLNEEGGQIAVFDATNTTRERRDLILDFAQENAYKVFFVESVCDDPEVIAANILEVKVSSPDYPEAHRERVMDDFLKRIECYKVTYQPLDPDEYDKDLSFIKVINVGRRFLVNRVQDYIQSKIVYYLMNIHVHSHSIYLCRHGESEHNIQGRIGGDSELSSRGRQFASALHEFVEEHKLSNLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGVCEEMTYEMIQNTFPEEFALRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSADDLAYLLCPLHTVMKLTPVAYGCKVEMFHLNVEAVNTHRDRPLQGKVRRDCAPLLLRRNSYTPLSSHDQVKRPRLYSAGNRPWLPLTPSPTTLHFPNTPDGALLLQQSQVSDSLNKLRNLTGTQCCPDTNPPSHWCSESPRNVTLSTDRA

Target ORF information:

RefSeq Version	XM_005155842.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155842.3

ORF Insert Sequence:

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAAGTTAGT 
GACAGTTTAA ACAAGCTCCG TAATCTTACA GGGACTCAGT GTTGTCCTGA CACTAACCCA 
CCCTCACACT GGTGTTCTGA ATCACCCCGG AACGTCACAC TTAGTACTGA TCGTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2b ( XM_005155842.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155899.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16998
Clone ID Related Accession (Same CDS sequence)	XM_005155842.3 , XM_005155842.4
Accession Version	XM_005155842.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155842.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAAGTTAGT 
GACAGTTTAA ACAAGCTCCG TAATCTTACA GGGACTCAGT GTTGTCCTGA CACTAACCCA 
CCCTCACACT GGTGTTCTGA ATCACCCCGG AACGTCACAC TTAGTACTGA TCGTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155899.1
CDS	218..1897
Translation	MSAWLQQEEAGPTSSAEAKKSEPRGQEKKCSWASYMTNSPTVIVMIGLPARGKTYMSKKLTRYLNWIGVPTKVFNLGVYRREAVKAYKSYDFFRHDNEEAMKIRKQCALVALQDVKIYLNEEGGQIAVFDATNTTRERRDLILDFAQENAYKVFFVESVCDDPEVIAANILEVKVSSPDYPEAHRERVMDDFLKRIECYKVTYQPLDPDEYDKDLSFIKVINVGRRFLVNRVQDYIQSKIVYYLMNIHVHSHSIYLCRHGESEHNIQGRIGGDSELSSRGRQFASALHEFVEEHKLSNLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGVCEEMTYEMIQNTFPEEFALRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSADDLAYLLCPLHTVMKLTPVAYGCKVEMFHLNVEAVNTHRDRPLQGKVRRDCAPLLLRRNSYTPLSSHDQVKRPRLYSAGNRPWLPLTPSPTTLHFPNTPDGALLLQQSQVSDSLNKLRNLTGTQCCPDTNPPSHWCSESPRNVTLSTDRA

Target ORF information:

RefSeq Version	XM_005155842.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155842.4

ORF Insert Sequence:

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAAGTTAGT 
GACAGTTTAA ACAAGCTCCG TAATCTTACA GGGACTCAGT GTTGTCCTGA CACTAACCCA 
CCCTCACACT GGTGTTCTGA ATCACCCCGG AACGTCACAC TTAGTACTGA TCGTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2b ( XM_005155843.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155900.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16999
Clone ID Related Accession (Same CDS sequence)	XM_005155843.3 , XM_005155843.4
Accession Version	XM_005155843.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAATCTCGT 
GTGGTTGGCA GCCATCTGAC TCCGTGTGTG AAGGAATCAA CTTTGACTGC TCAGACGAAG 
TTAATGACTG TTTCCGCTTC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155900.1
CDS	213..1859
Translation	MSAWLQQEEAGPTSSAEAKKSEPRGQEKKCSWASYMTNSPTVIVMIGLPARGKTYMSKKLTRYLNWIGVPTKVFNLGVYRREAVKAYKSYDFFRHDNEEAMKIRKQCALVALQDVKIYLNEEGGQIAVFDATNTTRERRDLILDFAQENAYKVFFVESVCDDPEVIAANILEVKVSSPDYPEAHRERVMDDFLKRIECYKVTYQPLDPDEYDKDLSFIKVINVGRRFLVNRVQDYIQSKIVYYLMNIHVHSHSIYLCRHGESEHNIQGRIGGDSELSSRGRQFASALHEFVEEHKLSNLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGVCEEMTYEMIQNTFPEEFALRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSADDLAYLLCPLHTVMKLTPVAYGCKVEMFHLNVEAVNTHRDRPLQGKVRRDCAPLLLRRNSYTPLSSHDQVKRPRLYSAGNRPWLPLTPSPTTLHFPNTPDGALLLQQSQSRVVGSHLTPCVKESTLTAQTKLMTVSASE

Target ORF information:

RefSeq Version	XM_005155843.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155843.3

ORF Insert Sequence:

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAATCTCGT 
GTGGTTGGCA GCCATCTGAC TCCGTGTGTG AAGGAATCAA CTTTGACTGC TCAGACGAAG 
TTAATGACTG TTTCCGCTTC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2b ( XM_005155843.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155900.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16999
Clone ID Related Accession (Same CDS sequence)	XM_005155843.3 , XM_005155843.4
Accession Version	XM_005155843.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155843.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAATCTCGT 
GTGGTTGGCA GCCATCTGAC TCCGTGTGTG AAGGAATCAA CTTTGACTGC TCAGACGAAG 
TTAATGACTG TTTCCGCTTC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155900.1
CDS	217..1863
Translation	MSAWLQQEEAGPTSSAEAKKSEPRGQEKKCSWASYMTNSPTVIVMIGLPARGKTYMSKKLTRYLNWIGVPTKVFNLGVYRREAVKAYKSYDFFRHDNEEAMKIRKQCALVALQDVKIYLNEEGGQIAVFDATNTTRERRDLILDFAQENAYKVFFVESVCDDPEVIAANILEVKVSSPDYPEAHRERVMDDFLKRIECYKVTYQPLDPDEYDKDLSFIKVINVGRRFLVNRVQDYIQSKIVYYLMNIHVHSHSIYLCRHGESEHNIQGRIGGDSELSSRGRQFASALHEFVEEHKLSNLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGVCEEMTYEMIQNTFPEEFALRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSADDLAYLLCPLHTVMKLTPVAYGCKVEMFHLNVEAVNTHRDRPLQGKVRRDCAPLLLRRNSYTPLSSHDQVKRPRLYSAGNRPWLPLTPSPTTLHFPNTPDGALLLQQSQSRVVGSHLTPCVKESTLTAQTKLMTVSASE

Target ORF information:

RefSeq Version	XM_005155843.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2b (pfkfb2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155843.4

ORF Insert Sequence:

ATGTCTGCAT GGCTGCAGCA GGAGGAGGCT GGTCCAACCA GTTCTGCTGA AGCCAAGAAA 
TCAGAGCCTA GAGGCCAAGA GAAGAAATGC TCATGGGCTT CTTACATGAC AAACTCCCCT 
ACTGTGATCG TCATGATCGG CCTCCCTGCC AGAGGCAAGA CGTACATGTC GAAAAAACTC 
ACTCGCTACC TTAACTGGAT AGGAGTTCCA ACAAAGGTGT TCAATCTGGG TGTGTACCGG 
AGGGAAGCAG TTAAAGCGTA CAAGTCATAT GACTTCTTTA GACATGACAA TGAGGAGGCC 
ATGAAAATAC GGAAACAGTG TGCATTGGTG GCTTTGCAAG ATGTCAAGAT CTACCTGAAT 
GAGGAAGGTG GACAAATAGC TGTATTTGAT GCCACAAACA CAACTCGGGA GAGGAGGGAT 
CTCATATTGG ATTTTGCACA GGAGAATGCA TACAAGGTGT TTTTTGTGGA GTCAGTATGT 
GATGATCCAG AGGTTATTGC TGCTAACATC CTGGAAGTTA AAGTTTCAAG TCCGGATTAT 
CCAGAAGCAC ACAGAGAGAG AGTCATGGAT GACTTCCTGA AACGTATTGA ATGTTATAAG 
GTCACTTACC AGCCGCTAGA TCCTGATGAA TATGACAAAG ACCTGTCCTT CATTAAGGTG 
ATAAATGTGG GTCGTCGATT CCTGGTGAAC CGTGTTCAGG ACTACATCCA GAGCAAGATT 
GTCTACTACC TCATGAACAT CCATGTACAC TCGCACTCTA TCTACCTGTG CAGGCATGGA 
GAGAGTGAAC ACAACATCCA AGGGCGTATT GGAGGAGACT CTGAACTCTC CTCACGAGGG 
AGACAGTTTG CGAGCGCCCT GCATGAGTTT GTTGAGGAGC ATAAGCTATC TAACCTGAAG 
GTTTGGACAA GCCAGTTAAG GAGAACCATC CAGACGGCTG AGGAGCTGGG AGTTCCCTAT 
GAACAGTGGA AAATCCTCAA TGAGATAGAC GCTGGTGTTT GTGAAGAGAT GACCTATGAA 
ATGATCCAGA ACACATTCCC AGAGGAATTT GCTTTAAGAG ATCAGGACAA GTACCATTAC 
AGATATCCAG GAGGAGAGTC ATATCAGGAC CTGGTTCAGC GACTGGAGCC TGTAATTATG 
GAGCTTGAGA GACAAGGCAA TGTACTAGTC ATCTGCCATC AGGCGGTAAT GCGGTGCCTA 
CTAGCCTATT TCTTGGACAA GAGTGCAGAT GATCTTGCCT ATTTGCTATG TCCTCTTCAT 
ACTGTCATGA AGCTCACTCC TGTTGCCTAC GGTTGCAAAG TTGAGATGTT TCATCTGAAT 
GTGGAGGCAG TGAACACACA TCGGGACAGA CCGCTGCAGG GGAAAGTGAG ACGAGACTGC 
GCTCCTCTGC TGTTAAGGCG GAATAGTTAC ACCCCTCTGT CCAGTCACGA CCAGGTTAAG 
CGTCCCCGTC TCTACAGCGC TGGCAACCGG CCCTGGCTGC CCCTCACCCC TTCCCCCACC 
ACCCTGCACT TCCCAAACAC ACCAGACGGG GCACTGCTGC TCCAGCAAAG CCAATCTCGT 
GTGGTTGGCA GCCATCTGAC TCCGTGTGTG AAGGAATCAA CTTTGACTGC TCAGACGAAG 
TTAATGACTG TTTCCGCTTC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pfkfb2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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