pfkfb2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pfkfb2a
Entrez Gene ID	393983
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a
Synonyms	pfkfb2,zgc:56282
General protein information	Preferred Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297125.2	XP_009295400.1	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1
XM_005170570.3	XP_005170627.1	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1
XM_005170570.4	XP_005170627.1	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1
XM_021469767.1	XP_021325442.1	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X2
NM_201008.1	NP_957302.1	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a
XM_009297125.3	XP_009295400.1	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06524	XP_003717027.1
Oryza sativa (rice)	Os03g0294200	NP_001049815.1
Mus musculus (house mouse)	Pfkfb2	NP_032851.2
Kluyveromyces lactis	KLLA0B07513g	XP_451863.1
Homo sapiens (human)	PFKFB2	NP_006203.2
Danio rerio (zebrafish)	pfkfb2a	NP_957302.1
Neurospora crassa	NCU09347	XP_958926.1
Schizosaccharomyces pombe (fission yeast)	SPAPB17E12.14c	NP_001018229.2
Pan troglodytes (chimpanzee)	PFKFB2	XP_001165997.2
Macaca mulatta (Rhesus monkey)	PFKFB2	XP_001082732.1
Eremothecium gossypii	AGOS_AEL106W	NP_984755.2
Gallus gallus (chicken)	PFKFB2	XP_004935023.1
Canis lupus familiaris (dog)	PFKFB2	XP_005622364.1
Bos taurus (cattle)	PFKFB2	NP_777237.3
Rattus norvegicus (Norway rat)	Pfkfb2	NP_001029136.1
Danio rerio (zebrafish)	LOC564852	XP_001919792.3
Saccharomyces cerevisiae (baker's yeast)	FBP26	NP_012380.1
Xenopus tropicalis (tropical clawed frog)	pfkfb2	NP_001123395.1

Gene Ontology
Bibliography

Related articles in PubMed

[Generation and phenotype analysis of zebrafish mutations of obesity-related genes lepr and mc4r].
Fei F, Sun SY, Yao YX, Wang X
Sheng li xue bao : [Acta physiologica Sinica]69(1)61-69(2017 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
Wu CC, Tsai TH, Chang C, Lee TT, Lin C, Cheng IH, Sun MC, Chuang YJ, Chen BS
PloS one9(6)e97902(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pfkfb2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfkfb2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151453	XM_021469767.1 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa31767	NM_201008.1 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa28105	XM_005170570.4 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa28105	XM_009297125.3 Latest version!	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa28105	XM_005170570.3	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa28105	XM_009297125.2	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pfkfb2a ( XM_021469767.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325442.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa151453
Clone ID Related Accession (Same CDS sequence)	XM_021469767.1
Accession Version	XM_021469767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAACT CACCCACAGT GATAGTGTTG GTTGGTCTTC CTGCCAGAGG AAAGACGTAT 
ATCTCCAAGA AGCTGACGCG ATACCTTAAC TGGATCGGTG TTCCTACTAA AGTTTTTAAC 
CTGGGTGTTT ATAGAAGAGA AGCTGTGCAG TCCTACAAGT CCTATGACTT CTTCCGTCAC 
GACAATGAAG AGGCCATGAA GATCAGGAGA CAGTGCGCCA TTGTGGCTCT TCAGGACGTG 
AAGTCTTATC TGAACGAGGA GGGAGGCCAG ATCGCTGTTT TTGATGCTAC AAACACAACC 
AGAGAGAGAC GAGACCTCAT CTTGAGTTTT GCGCAGGATC ACTCCTATAA GGTGTTTTTT 
GTCGAGTCTC TTTGTAGCGA CCCAGAAGTC ATTGCTGCCA ATATAATGGA TGTGAAGGTT 
TCCAGTCCGG ATTATCCGGA GCGAGACAGA GAGAGCGTAA TGGAGGACTT CCTCAAGAGA 
ATCGAGTGCT ACAAAGTCAC ATATCAGCCC TTAGACCCCG ATGAACATGA CAAGGCGCTG 
TCCTTCATCC AGGTGATCAA CGTGGGTCAG CGTTTCCTGG TGAACCGCGT TCAGGATTAT 
ATTCAGAGTA AGATCGTCTA CTACCTCATG AACATCCACG TTCAGAAACA CTCCATCTAC 
CTCTGCCGAC ACGGAGAGAG TCAGCACAAC GTCCAGGGCT GCATCGGTGG AGACTCGGAG 
CTCTCCAGCA GAGGAAAAGA GTTCTCTAAA GCCCTGCGTG GGTTTCTGGA AACCCAGAAG 
ATCCCAGACC TGAAGGTCTG GACCAGCCAG CTTAGACGCA CGATTCAGAC GGCGGAAGAG 
CTGGGCGTCC CGTACGAACA GTGGAAAATC CTCAACGAGA TCGACGCTGG CATCTGTGAA 
GAAATGACGT ATGAGAAGAT TAAAGAAGCA TACCCGGATG AATATTCTCT CAGAGACCAG 
GACAAATATC ATTACAGATA CCCAGGAGGA GAGTCGTATC AGGATCTGGT CCAGCGTCTG 
GAGCCGGTCA TCATGGAGCT GGAGAGACAA GGAAACGTGC TGGTGATCTG CCATCAGGCT 
GTCATGCGCT GCCTGCTCGC ATACTTCCTG GACAAGAGTG CCAATGATCT GCCGTATCTG 
AAGTGTCCTC TCCATGCGGT GCTCAAACTC ACCCCTGTGG CTTATGGCTG TAAAGTGGAC 
ATGTTTGACC TGAAAGTAGA AGCTGTAAAC ACACACCGGG ACAGACCTCT TGGCAGGGGC 
AGACAGGTAG TGGCTCCGGC CTTGCTGAGA AGGAACAGTT TCACTCCTCT GTCCAGTCAG 
GATCAGGTGA AGCGGCCGAG GCTCTTCAGC GTGGGAAACC CGCCGCGGAT AACAAGCGCA 
CCTGCTTTAT CAACAACACG ATTCCCACAG ACACGGGAGA GCGAAGAGCT GAGCGAGAGC 
CAGGAGTTAC TGAACTGCTT CAGCCCATCA GACTCAAAAG AGGTCATCCA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325442.1
CDS	262..1758
Translation	MTNSPTVIVLVGLPARGKTYISKKLTRYLNWIGVPTKVFNLGVYRREAVQSYKSYDFFRHDNEEAMKIRRQCAIVALQDVKSYLNEEGGQIAVFDATNTTRERRDLILSFAQDHSYKVFFVESLCSDPEVIAANIMDVKVSSPDYPERDRESVMEDFLKRIECYKVTYQPLDPDEHDKALSFIQVINVGQRFLVNRVQDYIQSKIVYYLMNIHVQKHSIYLCRHGESQHNVQGCIGGDSELSSRGKEFSKALRGFLETQKIPDLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGICEEMTYEKIKEAYPDEYSLRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSANDLPYLKCPLHAVLKLTPVAYGCKVDMFDLKVEAVNTHRDRPLGRGRQVVAPALLRRNSFTPLSSQDQVKRPRLFSVGNPPRITSAPALSTTRFPQTRESEELSESQELLNCFSPSDSKEVIQE

Target ORF information:

RefSeq Version	XM_021469767.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469767.1

ORF Insert Sequence:

ATGACAAACT CACCCACAGT GATAGTGTTG GTTGGTCTTC CTGCCAGAGG AAAGACGTAT 
ATCTCCAAGA AGCTGACGCG ATACCTTAAC TGGATCGGTG TTCCTACTAA AGTTTTTAAC 
CTGGGTGTTT ATAGAAGAGA AGCTGTGCAG TCCTACAAGT CCTATGACTT CTTCCGTCAC 
GACAATGAAG AGGCCATGAA GATCAGGAGA CAGTGCGCCA TTGTGGCTCT TCAGGACGTG 
AAGTCTTATC TGAACGAGGA GGGAGGCCAG ATCGCTGTTT TTGATGCTAC AAACACAACC 
AGAGAGAGAC GAGACCTCAT CTTGAGTTTT GCGCAGGATC ACTCCTATAA GGTGTTTTTT 
GTCGAGTCTC TTTGTAGCGA CCCAGAAGTC ATTGCTGCCA ATATAATGGA TGTGAAGGTT 
TCCAGTCCGG ATTATCCGGA GCGAGACAGA GAGAGCGTAA TGGAGGACTT CCTCAAGAGA 
ATCGAGTGCT ACAAAGTCAC ATATCAGCCC TTAGACCCCG ATGAACATGA CAAGGCGCTG 
TCCTTCATCC AGGTGATCAA CGTGGGTCAG CGTTTCCTGG TGAACCGCGT TCAGGATTAT 
ATTCAGAGTA AGATCGTCTA CTACCTCATG AACATCCACG TTCAGAAACA CTCCATCTAC 
CTCTGCCGAC ACGGAGAGAG TCAGCACAAC GTCCAGGGCT GCATCGGTGG AGACTCGGAG 
CTCTCCAGCA GAGGAAAAGA GTTCTCTAAA GCCCTGCGTG GGTTTCTGGA AACCCAGAAG 
ATCCCAGACC TGAAGGTCTG GACCAGCCAG CTTAGACGCA CGATTCAGAC GGCGGAAGAG 
CTGGGCGTCC CGTACGAACA GTGGAAAATC CTCAACGAGA TCGACGCTGG CATCTGTGAA 
GAAATGACGT ATGAGAAGAT TAAAGAAGCA TACCCGGATG AATATTCTCT CAGAGACCAG 
GACAAATATC ATTACAGATA CCCAGGAGGA GAGTCGTATC AGGATCTGGT CCAGCGTCTG 
GAGCCGGTCA TCATGGAGCT GGAGAGACAA GGAAACGTGC TGGTGATCTG CCATCAGGCT 
GTCATGCGCT GCCTGCTCGC ATACTTCCTG GACAAGAGTG CCAATGATCT GCCGTATCTG 
AAGTGTCCTC TCCATGCGGT GCTCAAACTC ACCCCTGTGG CTTATGGCTG TAAAGTGGAC 
ATGTTTGACC TGAAAGTAGA AGCTGTAAAC ACACACCGGG ACAGACCTCT TGGCAGGGGC 
AGACAGGTAG TGGCTCCGGC CTTGCTGAGA AGGAACAGTT TCACTCCTCT GTCCAGTCAG 
GATCAGGTGA AGCGGCCGAG GCTCTTCAGC GTGGGAAACC CGCCGCGGAT AACAAGCGCA 
CCTGCTTTAT CAACAACACG ATTCCCACAG ACACGGGAGA GCGAAGAGCT GAGCGAGAGC 
CAGGAGTTAC TGAACTGCTT CAGCCCATCA GACTCAAAAG AGGTCATCCA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2a ( NM_201008.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957302.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), mRNA.

Price & Availability↑

CloneID	ODa31767
Clone ID Related Accession (Same CDS sequence)	NM_201008.1
Accession Version	NM_201008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1563552000000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050514.1. ##Evidence-Data-START## Transcript exon combination :: BC050514.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCGCCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTACGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TAAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATACCAGC CCTTAGACCC CGGTGAACAT 
GACAAGGCGC TGTCCTTTAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG GCACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAAGTC TGGACCAGTC AGCTGAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCTTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGTC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAG AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957302.1
CDS	191..1759
Translation	MDSSNEHKKTEPRVTEKKCSWASHMTNSPTVIVLVGLPARGKTYISKKLTRYLNWIGVPTKVFNLGVYRREAVQSYKSYDFFRHDNEEAMKIRRQCAIVALQDVKSYLNEEGGQIAVFDATNTTRERRDLILSFAQDHSYKVFFVESLCSDPEVIAANIMDVKVSSPDYPERDRESVMEDFLKRIECYKVTYQPLDPGEHDKALSFIQVINVGQRFLVNRVQDYIQSKIVYYLMNIHVQKHSIYLCRHGESQHNVQGCIGGDSELSSRGKEFSKALRGFLETQKIPDLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGICEEMSYEKIKEAYPDEYSLRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSANDLPYLKCPLHAVLKLTPVAYGCKVDMFDLKVEAVNTHRDRPLGRGRQVVAPALLRRNSFTPLSSQDQVKRPRLFSVGNPPRITSAPALSTTRFPETRESEELSESQELLNCFSPSDSKEVIQE

Target ORF information:

RefSeq Version	NM_201008.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201008.1

ORF Insert Sequence:

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCGCCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTACGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TAAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATACCAGC CCTTAGACCC CGGTGAACAT 
GACAAGGCGC TGTCCTTTAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG GCACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAAGTC TGGACCAGTC AGCTGAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCTTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGTC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAG AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2a ( XM_009297125.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295400.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28105
Clone ID Related Accession (Same CDS sequence)	XM_009297125.2 , XM_005170570.3 , XM_005170570.4 , XM_009297125.3
Accession Version	XM_009297125.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295400.1
CDS	316..1884
Translation	MDSSNEHKKTEPRVTEKKCSWASHMTNSPTVIVLVGLPARGKTYISKKLTRYLNWIGVPTKVFNLGVYRREAVQSYKSYDFFRHDNEEAMKIRRQCAIVALQDVKSYLNEEGGQIAVFDATNTTRERRDLILSFAQDHSYKVFFVESLCSDPEVIAANIMDVKVSSPDYPERDRESVMEDFLKRIECYKVTYQPLDPDEHDKALSFIQVINVGQRFLVNRVQDYIQSKIVYYLMNIHVQKHSIYLCRHGESQHNVQGCIGGDSELSSRGKEFSKALRGFLETQKIPDLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGICEEMTYEKIKEAYPDEYSLRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSANDLPYLKCPLHAVLKLTPVAYGCKVDMFDLKVEAVNTHRDRPLGRGRQVVAPALLRRNSFTPLSSQDQVKRPRLFSVGNPPRITSAPALSTTRFPQTRESEELSESQELLNCFSPSDSKEVIQE

Target ORF information:

RefSeq Version	XM_009297125.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297125.2

ORF Insert Sequence:

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2a ( XM_005170570.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170627.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28105
Clone ID Related Accession (Same CDS sequence)	XM_009297125.2 , XM_005170570.3 , XM_005170570.4 , XM_009297125.3
Accession Version	XM_005170570.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170627.1
CDS	271..1839
Translation	MDSSNEHKKTEPRVTEKKCSWASHMTNSPTVIVLVGLPARGKTYISKKLTRYLNWIGVPTKVFNLGVYRREAVQSYKSYDFFRHDNEEAMKIRRQCAIVALQDVKSYLNEEGGQIAVFDATNTTRERRDLILSFAQDHSYKVFFVESLCSDPEVIAANIMDVKVSSPDYPERDRESVMEDFLKRIECYKVTYQPLDPDEHDKALSFIQVINVGQRFLVNRVQDYIQSKIVYYLMNIHVQKHSIYLCRHGESQHNVQGCIGGDSELSSRGKEFSKALRGFLETQKIPDLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGICEEMTYEKIKEAYPDEYSLRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSANDLPYLKCPLHAVLKLTPVAYGCKVDMFDLKVEAVNTHRDRPLGRGRQVVAPALLRRNSFTPLSSQDQVKRPRLFSVGNPPRITSAPALSTTRFPQTRESEELSESQELLNCFSPSDSKEVIQE

Target ORF information:

RefSeq Version	XM_005170570.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170570.3

ORF Insert Sequence:

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2a ( XM_005170570.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170627.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28105
Clone ID Related Accession (Same CDS sequence)	XM_009297125.2 , XM_005170570.3 , XM_005170570.4 , XM_009297125.3
Accession Version	XM_005170570.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170570.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170627.1
CDS	319..1887
Translation	MDSSNEHKKTEPRVTEKKCSWASHMTNSPTVIVLVGLPARGKTYISKKLTRYLNWIGVPTKVFNLGVYRREAVQSYKSYDFFRHDNEEAMKIRRQCAIVALQDVKSYLNEEGGQIAVFDATNTTRERRDLILSFAQDHSYKVFFVESLCSDPEVIAANIMDVKVSSPDYPERDRESVMEDFLKRIECYKVTYQPLDPDEHDKALSFIQVINVGQRFLVNRVQDYIQSKIVYYLMNIHVQKHSIYLCRHGESQHNVQGCIGGDSELSSRGKEFSKALRGFLETQKIPDLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGICEEMTYEKIKEAYPDEYSLRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSANDLPYLKCPLHAVLKLTPVAYGCKVDMFDLKVEAVNTHRDRPLGRGRQVVAPALLRRNSFTPLSSQDQVKRPRLFSVGNPPRITSAPALSTTRFPQTRESEELSESQELLNCFSPSDSKEVIQE

Target ORF information:

RefSeq Version	XM_005170570.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170570.4

ORF Insert Sequence:

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkfb2a ( XM_009297125.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295400.1 Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28105
Clone ID Related Accession (Same CDS sequence)	XM_009297125.2 , XM_005170570.3 , XM_005170570.4 , XM_009297125.3
Accession Version	XM_009297125.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295400.1
CDS	364..1932
Translation	MDSSNEHKKTEPRVTEKKCSWASHMTNSPTVIVLVGLPARGKTYISKKLTRYLNWIGVPTKVFNLGVYRREAVQSYKSYDFFRHDNEEAMKIRRQCAIVALQDVKSYLNEEGGQIAVFDATNTTRERRDLILSFAQDHSYKVFFVESLCSDPEVIAANIMDVKVSSPDYPERDRESVMEDFLKRIECYKVTYQPLDPDEHDKALSFIQVINVGQRFLVNRVQDYIQSKIVYYLMNIHVQKHSIYLCRHGESQHNVQGCIGGDSELSSRGKEFSKALRGFLETQKIPDLKVWTSQLRRTIQTAEELGVPYEQWKILNEIDAGICEEMTYEKIKEAYPDEYSLRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVICHQAVMRCLLAYFLDKSANDLPYLKCPLHAVLKLTPVAYGCKVDMFDLKVEAVNTHRDRPLGRGRQVVAPALLRRNSFTPLSSQDQVKRPRLFSVGNPPRITSAPALSTTRFPQTRESEELSESQELLNCFSPSDSKEVIQE

Target ORF information:

RefSeq Version	XM_009297125.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a (pfkfb2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297125.3

ORF Insert Sequence:

ATGGACAGTT CGAATGAGCA CAAGAAGACG GAGCCCAGAG TCACTGAGAA GAAATGCTCA 
TGGGCCTCAC ACATGACAAA CTCACCCACA GTGATAGTGT TGGTTGGTCT TCCTGCCAGA 
GGAAAGACGT ATATCTCCAA GAAGCTGACG CGATACCTTA ACTGGATCGG TGTTCCTACT 
AAAGTTTTTA ACCTGGGTGT TTATAGAAGA GAAGCTGTGC AGTCCTACAA GTCCTATGAC 
TTCTTCCGTC ACGACAATGA AGAGGCCATG AAGATCAGGA GACAGTGCGC CATTGTGGCT 
CTTCAGGACG TGAAGTCTTA TCTGAACGAG GAGGGAGGCC AGATCGCTGT TTTTGATGCT 
ACAAACACAA CCAGAGAGAG ACGAGACCTC ATCTTGAGTT TTGCGCAGGA TCACTCCTAT 
AAGGTGTTTT TTGTCGAGTC TCTTTGTAGC GACCCAGAAG TCATTGCTGC CAATATAATG 
GATGTGAAGG TTTCCAGTCC GGATTATCCG GAGCGAGACA GAGAGAGCGT AATGGAGGAC 
TTCCTCAAGA GAATCGAGTG CTACAAAGTC ACATATCAGC CCTTAGACCC CGATGAACAT 
GACAAGGCGC TGTCCTTCAT CCAGGTGATC AACGTGGGTC AGCGTTTCCT GGTGAACCGC 
GTTCAGGATT ATATTCAGAG TAAGATCGTC TACTACCTCA TGAACATCCA CGTTCAGAAA 
CACTCCATCT ACCTCTGCCG ACACGGAGAG AGTCAGCACA ACGTCCAGGG CTGCATCGGT 
GGAGACTCGG AGCTCTCCAG CAGAGGAAAA GAGTTCTCTA AAGCCCTGCG TGGGTTTCTG 
GAAACCCAGA AGATCCCAGA CCTGAAGGTC TGGACCAGCC AGCTTAGACG CACGATTCAG 
ACGGCGGAAG AGCTGGGCGT CCCGTACGAA CAGTGGAAAA TCCTCAACGA GATCGACGCT 
GGCATCTGTG AAGAAATGAC GTATGAGAAG ATTAAAGAAG CATACCCGGA TGAATATTCT 
CTCAGAGACC AGGACAAATA TCATTACAGA TACCCAGGAG GAGAGTCGTA TCAGGATCTG 
GTCCAGCGTC TGGAGCCGGT CATCATGGAG CTGGAGAGAC AAGGAAACGT GCTGGTGATC 
TGCCATCAGG CTGTCATGCG CTGCCTGCTC GCATACTTCC TGGACAAGAG TGCCAATGAT 
CTGCCGTATC TGAAGTGTCC TCTCCATGCG GTGCTCAAAC TCACCCCTGT GGCTTATGGC 
TGTAAAGTGG ACATGTTTGA CCTGAAAGTA GAAGCTGTAA ACACACACCG GGACAGACCT 
CTTGGCAGGG GCAGACAGGT AGTGGCTCCG GCCTTGCTGA GAAGGAACAG TTTCACTCCT 
CTGTCCAGTC AGGATCAGGT GAAGCGGCCG AGGCTCTTCA GCGTGGGAAA CCCGCCGCGG 
ATAACAAGCG CACCTGCTTT ATCAACAACA CGATTCCCAC AGACACGGGA GAGCGAAGAG 
CTGAGCGAGA GCCAGGAGTT ACTGAACTGC TTCAGCCCAT CAGACTCAAA AGAGGTCATC 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Generation and phenotype analysis of zebrafish mutations of obesity-related genes lepr and mc4r].
Sheng li xue bao : [Acta physiologica Sinica]69(1)61-69(2017 Feb)
Fei F,Sun SY,Yao YX,Wang X

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
PloS one9(6)e97902(2014)
Wu CC,Tsai TH,Chang C,Lee TT,Lin C,Cheng IH,Sun MC,Chuang YJ,Chen BS

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pfkfb2a cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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