capn2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	capn2b
Entrez Gene ID	563053
Full Name	calpain 2, (m/II) large subunit b
Synonyms	im:6908877,zgc:109906
General protein information	Preferred Names calpain 2, (m/II) large subunit b Names calpain 2, (m/II) large subunit b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018227.1	NP_001018177.1	calpain 2, (m/II) large subunit b

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	capn2a	NP_001013519.1
Danio rerio (zebrafish)	capn2b	NP_001018177.1

Gene Ontology
Bibliography

Related articles in PubMed

PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components.
Ma Z, Zhu P, Shi H, Guo L, Zhang Q, Chen Y, Chen S, Zhang Z, Peng J, Chen J
Nature568(7751)259-263(2019 04)

Capn5 Expression in the Healthy and Regenerating Zebrafish Retina.
Coomer CE, Morris AC
Investigative ophthalmology & visual science59(8)3643-3654(2018 07)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Bugel SM, Wehmas LC, La Du JK, Tanguay RL
Toxicology and applied pharmacology29631-41(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following capn2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the capn2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37177	NM_001018227.1 Latest version!	Danio rerio calpain 2, (m/II) large subunit b (capn2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

capn2b ( NM_001018227.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018177.1 Danio rerio calpain 2, (m/II) large subunit b (capn2b), mRNA.

Price & Availability↑

CloneID	ODa37177
Clone ID Related Accession (Same CDS sequence)	NM_001018227.1
Accession Version	NM_001018227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565971200000
Organism	Danio rerio(zebrafish)
Product	calpain 2, (m/II) large subunit b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095045.1. On Jan 12, 2008 this sequence version replaced XM_693145.2. ##Evidence-Data-START## Transcript exon combination :: BC095045.1, BC171423.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGGAA TTGCGTCTAC TGTGGCCAAG AAGAGAGCGC TGGCTGCAGG GTTTGGAACC 
AACGCCAATG CCACCAAGTA CCTGAACCAG GACTTTGAGG CGCTCAGGAG TGAGTGCAGG 
GCCAGAGGGA CCCTGTTCTC AGACCCGACT TTCCCCGCCG CTCCTGAATC ACTGGGCTTT 
AAAGAACTCG GACCCAACTC GTCCAAAACC AGAGGATTAG TATGGAAAAG GCCCGGGGAA 
TTATGCCAGA AACCACGCTT CATCGTTGGT GGGGCCACAA AAACGGATAT CTGTCAAGGA 
GCTTTGGGTG ACTGTTGGCT GCTGGCTGCT ATTGCCTCCC TGACACAGAA TGAAGATGTT 
TTGGCAAGAG TTGTGCCCAA TGGACAGGAG TTTGATGGCA CTTATGCAGG AATCTTCCAT 
TTCCAGTTCT GGCAGTTTGG CGAGTGGGTG GATGTGGTCA TTGACGACCG GCTGCCGGTG 
AAAGATGGCG AGCTGTTGTT TGTTTACTCC GCTGAGAAAA ACGAGTTCTG GAGCGCCCTG 
CTGGAGAAGG CCTATGCTAA GGTGAACGGC TGTTATGAAG CCCTGTCTGG AGGATCCACT 
AGCGAGGGTT TTGAGGACTT CACTGGAGGC ATCGCTGAGA GCTACGAGAT CAGAAAAGCA 
CCGACCAATT TGTTTCAGAT CATCCAGAAA GCCCTGGAGG CTGGAGCTTT GCTGGGCTGC 
TCCATCGACA TCACTAGTGC TGCTGATTCA GAAGCCATCA CTCGTCAGAA GCTGGTCAAA 
GGCCACGCCT ACTCACTCAC AGGGGCAACA GAGGTGAACT ATCGTGGTCG CAAGGAAAAG 
CTGGTTCGTA TGCGCAACCC TTGGGGACAG GTGGAGTGGA CCGGAGCCTG GAGCGACAAT 
TCACCTGAAT GGAACAGTGT TGCGGCATCA GAGCGTCCAG ACGCCAGCGC CGAGGATGGT 
GAATTTTGGA TGGCATTCTC CGAATTCCTC ACCAATTACT CTCGTATAGA GATCTGCACT 
CTGACGCCGG ATGCCATCAC AGATGATTCA GTAAAACATT GGGCCGTGAC AAATCATGAC 
GGCACATGGA GGAGAGGCTC CACCGCTGGA GGCTGCAGAA ACAATCCATA CACATTTTGG 
ATGAATCCAC AATTTGTGGT GAAACTGGAT GAAGAAAATG TCGAAGATAA GGAGAAGGGC 
TGCAGTTTTG TAGTGGGTCT GATTCAGAAG AACCGCCGGC GCCTGAGGAA AACTGGAGAG 
GATATGAACA CTATTGGCTT TGCTATTTAT GAGCTCCCCC AGGAGTTTCA CGGTCAGCGG 
GAAGTGCACC TGGATAAAAA CTTCTTCCTG ACGCACGGAC AGAAAGCTCG CTCTGAGACT 
TTCATTAACC TCAGGGAGGT CTGCACGCGC TTCCATCTGC CTCCTGGAGA GTACCTCATC 
GTACCATCTA CCTTCGAAGC CAACAAGGAC GGAGACTTCT GCCTGCGTGT CTTCTCTGAA 
AGCCAAAGTG AAACACAACA TTGCGATGAC CCTGTGGATG CTGAATTACC TGATGAGACT 
GTCTCAGATG ATCAAGTTGA TTCAAAGTTT AGGGGGATGT TTGTAAAACT TGCCGGACCT 
GACATGGAAA TCTCGCCCCT GGAGCTCATG ACCATCTTTA ACAAAATCAT TGCAAAACGC 
ACTGACATTA AGTCGAGCAC CTTCACTTTG GATACGTGTC GTGTCATGGT GAACCTCATG 
GACGACAGTG GGAATGGAAA ACTGGGGCTG GGAGAGTTTG CTACTCTGTG GAAGAAAGTG 
CAGAGATACC TTGAAATTTT CAAGCATAAT GACTTGGATA GCTCGGGAAC CATAAGCACT 
CCAGAGCTGC GTATGGCACT GAAAGAGGCC GGTTTCTGTC TCAATAACAC TTTATTTCAG 
CTGATGGTTG CACGTTATGC TGAGAAGGAC ATGACTCTCC TCTTTGACAA CTTCGTGTCC 
TGCCTGATGC GTCTTGAAAT GATGTTCAGG GCATTCAAAA GGTTGGATCC TCACAAAAGT 
GGCTTCATTG AGCTGAATTT TAATCAGTGG CTGAACCTTA CCATGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018177.1
CDS	73..2163
Translation	MAGIASTVAKKRALAAGFGTNANATKYLNQDFEALRSECRARGTLFSDPTFPAAPESLGFKELGPNSSKTRGLVWKRPGELCQKPRFIVGGATKTDICQGALGDCWLLAAIASLTQNEDVLARVVPNGQEFDGTYAGIFHFQFWQFGEWVDVVIDDRLPVKDGELLFVYSAEKNEFWSALLEKAYAKVNGCYEALSGGSTSEGFEDFTGGIAESYEIRKAPTNLFQIIQKALEAGALLGCSIDITSAADSEAITRQKLVKGHAYSLTGATEVNYRGRKEKLVRMRNPWGQVEWTGAWSDNSPEWNSVAASERPDASAEDGEFWMAFSEFLTNYSRIEICTLTPDAITDDSVKHWAVTNHDGTWRRGSTAGGCRNNPYTFWMNPQFVVKLDEENVEDKEKGCSFVVGLIQKNRRRLRKTGEDMNTIGFAIYELPQEFHGQREVHLDKNFFLTHGQKARSETFINLREVCTRFHLPPGEYLIVPSTFEANKDGDFCLRVFSESQSETQHCDDPVDAELPDETVSDDQVDSKFRGMFVKLAGPDMEISPLELMTIFNKIIAKRTDIKSSTFTLDTCRVMVNLMDDSGNGKLGLGEFATLWKKVQRYLEIFKHNDLDSSGTISTPELRMALKEAGFCLNNTLFQLMVARYAEKDMTLLFDNFVSCLMRLEMMFRAFKRLDPHKSGFIELNFNQWLNLTMI

Target ORF information:

RefSeq Version	NM_001018227.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 2, (m/II) large subunit b (capn2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018227.1

ORF Insert Sequence:

ATGGCAGGAA TTGCGTCTAC TGTGGCCAAG AAGAGAGCGC TGGCTGCAGG GTTTGGAACC 
AACGCCAATG CCACCAAGTA CCTGAACCAG GACTTTGAGG CGCTCAGGAG TGAGTGCAGG 
GCCAGAGGGA CCCTGTTCTC AGACCCGACT TTCCCCGCCG CTCCTGAATC ACTGGGCTTT 
AAAGAACTCG GACCCAACTC GTCCAAAACC AGAGGATTAG TATGGAAAAG GCCCGGGGAA 
TTATGCCAGA AACCACGCTT CATCGTTGGT GGGGCCACAA AAACGGATAT CTGTCAAGGA 
GCTTTGGGTG ACTGTTGGCT GCTGGCTGCT ATTGCCTCCC TGACACAGAA TGAAGATGTT 
TTGGCAAGAG TTGTGCCCAA TGGACAGGAG TTTGATGGCA CTTATGCAGG AATCTTCCAT 
TTCCAGTTCT GGCAGTTTGG CGAGTGGGTG GATGTGGTCA TTGACGACCG GCTGCCGGTG 
AAAGATGGCG AGCTGTTGTT TGTTTACTCC GCTGAGAAAA ACGAGTTCTG GAGCGCCCTG 
CTGGAGAAGG CCTATGCTAA GGTGAACGGC TGTTATGAAG CCCTGTCTGG AGGATCCACT 
AGCGAGGGTT TTGAGGACTT CACTGGAGGC ATCGCTGAGA GCTACGAGAT CAGAAAAGCA 
CCGACCAATT TGTTTCAGAT CATCCAGAAA GCCCTGGAGG CTGGAGCTTT GCTGGGCTGC 
TCCATCGACA TCACTAGTGC TGCTGATTCA GAAGCCATCA CTCGTCAGAA GCTGGTCAAA 
GGCCACGCCT ACTCACTCAC AGGGGCAACA GAGGTGAACT ATCGTGGTCG CAAGGAAAAG 
CTGGTTCGTA TGCGCAACCC TTGGGGACAG GTGGAGTGGA CCGGAGCCTG GAGCGACAAT 
TCACCTGAAT GGAACAGTGT TGCGGCATCA GAGCGTCCAG ACGCCAGCGC CGAGGATGGT 
GAATTTTGGA TGGCATTCTC CGAATTCCTC ACCAATTACT CTCGTATAGA GATCTGCACT 
CTGACGCCGG ATGCCATCAC AGATGATTCA GTAAAACATT GGGCCGTGAC AAATCATGAC 
GGCACATGGA GGAGAGGCTC CACCGCTGGA GGCTGCAGAA ACAATCCATA CACATTTTGG 
ATGAATCCAC AATTTGTGGT GAAACTGGAT GAAGAAAATG TCGAAGATAA GGAGAAGGGC 
TGCAGTTTTG TAGTGGGTCT GATTCAGAAG AACCGCCGGC GCCTGAGGAA AACTGGAGAG 
GATATGAACA CTATTGGCTT TGCTATTTAT GAGCTCCCCC AGGAGTTTCA CGGTCAGCGG 
GAAGTGCACC TGGATAAAAA CTTCTTCCTG ACGCACGGAC AGAAAGCTCG CTCTGAGACT 
TTCATTAACC TCAGGGAGGT CTGCACGCGC TTCCATCTGC CTCCTGGAGA GTACCTCATC 
GTACCATCTA CCTTCGAAGC CAACAAGGAC GGAGACTTCT GCCTGCGTGT CTTCTCTGAA 
AGCCAAAGTG AAACACAACA TTGCGATGAC CCTGTGGATG CTGAATTACC TGATGAGACT 
GTCTCAGATG ATCAAGTTGA TTCAAAGTTT AGGGGGATGT TTGTAAAACT TGCCGGACCT 
GACATGGAAA TCTCGCCCCT GGAGCTCATG ACCATCTTTA ACAAAATCAT TGCAAAACGC 
ACTGACATTA AGTCGAGCAC CTTCACTTTG GATACGTGTC GTGTCATGGT GAACCTCATG 
GACGACAGTG GGAATGGAAA ACTGGGGCTG GGAGAGTTTG CTACTCTGTG GAAGAAAGTG 
CAGAGATACC TTGAAATTTT CAAGCATAAT GACTTGGATA GCTCGGGAAC CATAAGCACT 
CCAGAGCTGC GTATGGCACT GAAAGAGGCC GGTTTCTGTC TCAATAACAC TTTATTTCAG 
CTGATGGTTG CACGTTATGC TGAGAAGGAC ATGACTCTCC TCTTTGACAA CTTCGTGTCC 
TGCCTGATGC GTCTTGAAAT GATGTTCAGG GCATTCAAAA GGTTGGATCC TCACAAAAGT 
GGCTTCATTG AGCTGAATTT TAATCAGTGG CTGAACCTTA CCATGATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components.
Nature568(7751)259-263(2019 04)
Ma Z,Zhu P,Shi H,Guo L,Zhang Q,Chen Y,Chen S,Zhang Z,Peng J,Chen J

Capn5 Expression in the Healthy and Regenerating Zebrafish Retina.
Investigative ophthalmology & visual science59(8)3643-3654(2018 07)
Coomer CE,Morris AC

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Toxicology and applied pharmacology29631-41(2016 Apr)
Bugel SM,Wehmas LC,La Du JK,Tanguay RL

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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capn2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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