capn2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	capn2a
Entrez Gene ID	541374
Full Name	calpain 2, (m/II) large subunit a
Synonyms	zgc:113590
General protein information	Preferred Names calpain 2, (m/II) large subunit a Names calpain 2, (m/II) large subunit a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013501.2	NP_001013519.1	calpain 2, (m/II) large subunit a

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	capn2a	NP_001013519.1
Danio rerio (zebrafish)	capn2b	NP_001018177.1

Gene Ontology
Bibliography

Related articles in PubMed

PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components.
Ma Z, Zhu P, Shi H, Guo L, Zhang Q, Chen Y, Chen S, Zhang Z, Peng J, Chen J
Nature568(7751)259-263(2019 04)

Capn5 Expression in the Healthy and Regenerating Zebrafish Retina.
Coomer CE, Morris AC
Investigative ophthalmology & visual science59(8)3643-3654(2018 07)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Bugel SM, Wehmas LC, La Du JK, Tanguay RL
Toxicology and applied pharmacology29631-41(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following capn2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the capn2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31296	NM_001013501.2 Latest version!	Danio rerio calpain 2, (m/II) large subunit a (capn2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

capn2a ( NM_001013501.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001013519.1 Danio rerio calpain 2, (m/II) large subunit a (capn2a), mRNA.

Price & Availability↑

CloneID	ODa31296
Clone ID Related Accession (Same CDS sequence)	NM_001013501.2
Accession Version	NM_001013501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565971200000
Organism	Danio rerio(zebrafish)
Product	calpain 2, (m/II) large subunit a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC090435.1. On Aug 31, 2012 this sequence version replaced NM_001013501.1. ##Evidence-Data-START## Transcript exon combination :: BC090435.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGGCG TGGCATCCAC ACTCGCCAAG AAGAGAGCCC TGGCGGCTGG CTTTGGGACG 
AACTCCAATG CCGTGAAATA CCTGAACCAG AACTTCGAGA CTTTGAGGAG TGAGTGCCTG 
AGCCGCGGAG AGCTTTTCTG TGATCCCGCT TTCCCAGCCG CTCCCGAGGC CCTGGGCTTT 
AATGAACTCG GGCCACGGTC GTCAAAAACC AGAGGTGTTG AGTGGAAAAG ACCTGGGCAA 
TTGAATTCCA GTCCGGAGTT CATAGTTGGA GGAGCTACAA GAACAGATAT TTGCCAAGGA 
GCTTTAGGTG ATTGCTGGCT CTTAGCAGCC ATCGCCTCTC TGACCCTTAA TGAAGACGTT 
TTGGCTCGTG TCGTACCATC CGATCAGGGA TTTGGGCAAG ACTATGCTGG AATTTTCCAC 
TTTCAGTTTT GGCAGTTTGG AGAATGGGTG GATGTGGTGG TGGATGATCG ATTGCCCACT 
AGAGATGGAG AACTGCTCTT TGTTCACTCC GTCACAGGCT CTGAGTTCTG GAGCGCTCTG 
CTGGAGAAGG CCTATGCAAA AGTGAATGGA TGTTATGAAG CCCTTTCTGG AGGTTCCACC 
ACAGAAGGTT TTGAGGATTT CACTGGTGGG ATTGCAGAGA TGTACGAGCT GAAAAGTGCA 
CCGACCAACC TGTTTCAGAT CATCAAGAAG GCCCTGGACT CTGGAGCTCT GTTGGGATGC 
TCCATAGATA TCACCAGTGC TGCTGATTCT GAGGCCGTCA CTTATCAGAA GCTTGTAAAG 
GGACATGCAT ACTCTCTTAC TGGAGCTACT GAGGTAAATT ACAGAAGTCG AAAAGAAAAG 
CTGGTCCGAG TGCGTAATCC TTGGGGTCAG GTGGAATGGA CTGGGGCTTG GAGTGATAAC 
TCTTCTGAGT GGAACGCTGT CGATCCATCT GAGCGTGAGA ATGTGAAATC AGAAGATGGA 
GAGTTCTGGA TGTCATTCTC AGATTTTGTG AGGCAGTATT CCCGCCTTGA GATCTGCACT 
CTGACCCCAG ACACGCTGAC GTCTGACTCG CTCAAACACT GGAGTGTGTG TAAATTTGAT 
GGCACCTGGA GAAAAGGCTC TACGGCTGGT GGTTGCAGGA ACCATCCATA CACGTTCTGG 
ATGAATCCTC AGTTTAAGAT CAAGCTGGAG GAGGAAGATG ATGATCCGGA TGATAATGAA 
GTGGGCTGCA GTGTGGTGAT CGGCTTGATT CAGAAGAACC GCCGCAAGAT GAGGAAGGCT 
GGAGAAGACA TGCACACTAT CGGCTATGCC ATCTACGAGG TGCCTTCTCA GTTTCATGGG 
CAAAAGGAGG TTCATCTGGA TAAGAACTAT TTCCTGACTC ACGCTCAGAA AGCTCGATCT 
GAAACCTTTA TCAACCTCAG AGAGGTCAGC ACCCGCTTCA AACTTCCTCC TGGAGAATAT 
CTCATCGTCC CTTCTACATT CGATCCGCAC AAGAACGGGG ATTTTTGTGT GCGCGTGTTC 
TCCGAGAAAC AGTCTGAGAT GCAACAATGT GATGACCCTA TAGAAGCAAA TGTAGATGAT 
GAAATGGTGT CTGAAGAAGA GGTTGATTCT GGCTTTAGGG GCCTGTTTAC TAAACTTGCA 
GGAGATGACA TGGAGATCTC TGCTTCTGAG CTGCGAACGA TTTTCAACAA AATAGTGGCA 
AAGAGAACCG ATATCAAGAC TGATGGTTTC AGTCTGGATA CATGTAGGGT GATGGTCAAC 
CTCATGGATG AAAGTGGAAA TGGAAAACTA GGATTAACTG AGTTTGCTAC ACTTTGGAAA 
AAGATTCAAA AGTATCTGGG CATTTATAAG AAGAATGACA TGGATGGATC CGGCTGTATG 
AGTACACCAG AGATGCGAAT GGCTTTGAAG GAAGCAGGAT TCTCTCTGAA TGACTGCATT 
CACCAGAGTC TGGCTGCACG ATATGGAGAC GCAGACATGA CCATCGACTT TGACAACTTT 
GTGTCCTGTG TGATGCGCCT GGAGATGATG TTCAAAGTGT TCAAGAGGAT GGATATAGAC 
CACAGCGGGT TCATTGAGCT GGACTTCTTT CAGTGGCTGT CATTTGCTAT GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013519.1
CDS	86..2182
Translation	MSGVASTLAKKRALAAGFGTNSNAVKYLNQNFETLRSECLSRGELFCDPAFPAAPEALGFNELGPRSSKTRGVEWKRPGQLNSSPEFIVGGATRTDICQGALGDCWLLAAIASLTLNEDVLARVVPSDQGFGQDYAGIFHFQFWQFGEWVDVVVDDRLPTRDGELLFVHSVTGSEFWSALLEKAYAKVNGCYEALSGGSTTEGFEDFTGGIAEMYELKSAPTNLFQIIKKALDSGALLGCSIDITSAADSEAVTYQKLVKGHAYSLTGATEVNYRSRKEKLVRVRNPWGQVEWTGAWSDNSSEWNAVDPSERENVKSEDGEFWMSFSDFVRQYSRLEICTLTPDTLTSDSLKHWSVCKFDGTWRKGSTAGGCRNHPYTFWMNPQFKIKLEEEDDDPDDNEVGCSVVIGLIQKNRRKMRKAGEDMHTIGYAIYEVPSQFHGQKEVHLDKNYFLTHAQKARSETFINLREVSTRFKLPPGEYLIVPSTFDPHKNGDFCVRVFSEKQSEMQQCDDPIEANVDDEMVSEEEVDSGFRGLFTKLAGDDMEISASELRTIFNKIVAKRTDIKTDGFSLDTCRVMVNLMDESGNGKLGLTEFATLWKKIQKYLGIYKKNDMDGSGCMSTPEMRMALKEAGFSLNDCIHQSLAARYGDADMTIDFDNFVSCVMRLEMMFKVFKRMDIDHSGFIELDFFQWLSFAMI

Target ORF information:

RefSeq Version	NM_001013501.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 2, (m/II) large subunit a (capn2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013501.2

ORF Insert Sequence:

ATGTCCGGCG TGGCATCCAC ACTCGCCAAG AAGAGAGCCC TGGCGGCTGG CTTTGGGACG 
AACTCCAATG CCGTGAAATA CCTGAACCAG AACTTCGAGA CTTTGAGGAG TGAGTGCCTG 
AGCCGCGGAG AGCTTTTCTG TGATCCCGCT TTCCCAGCCG CTCCCGAGGC CCTGGGCTTT 
AATGAACTCG GGCCACGGTC GTCAAAAACC AGAGGTGTTG AGTGGAAAAG ACCTGGGCAA 
TTGAATTCCA GTCCGGAGTT CATAGTTGGA GGAGCTACAA GAACAGATAT TTGCCAAGGA 
GCTTTAGGTG ATTGCTGGCT CTTAGCAGCC ATCGCCTCTC TGACCCTTAA TGAAGACGTT 
TTGGCTCGTG TCGTACCATC CGATCAGGGA TTTGGGCAAG ACTATGCTGG AATTTTCCAC 
TTTCAGTTTT GGCAGTTTGG AGAATGGGTG GATGTGGTGG TGGATGATCG ATTGCCCACT 
AGAGATGGAG AACTGCTCTT TGTTCACTCC GTCACAGGCT CTGAGTTCTG GAGCGCTCTG 
CTGGAGAAGG CCTATGCAAA AGTGAATGGA TGTTATGAAG CCCTTTCTGG AGGTTCCACC 
ACAGAAGGTT TTGAGGATTT CACTGGTGGG ATTGCAGAGA TGTACGAGCT GAAAAGTGCA 
CCGACCAACC TGTTTCAGAT CATCAAGAAG GCCCTGGACT CTGGAGCTCT GTTGGGATGC 
TCCATAGATA TCACCAGTGC TGCTGATTCT GAGGCCGTCA CTTATCAGAA GCTTGTAAAG 
GGACATGCAT ACTCTCTTAC TGGAGCTACT GAGGTAAATT ACAGAAGTCG AAAAGAAAAG 
CTGGTCCGAG TGCGTAATCC TTGGGGTCAG GTGGAATGGA CTGGGGCTTG GAGTGATAAC 
TCTTCTGAGT GGAACGCTGT CGATCCATCT GAGCGTGAGA ATGTGAAATC AGAAGATGGA 
GAGTTCTGGA TGTCATTCTC AGATTTTGTG AGGCAGTATT CCCGCCTTGA GATCTGCACT 
CTGACCCCAG ACACGCTGAC GTCTGACTCG CTCAAACACT GGAGTGTGTG TAAATTTGAT 
GGCACCTGGA GAAAAGGCTC TACGGCTGGT GGTTGCAGGA ACCATCCATA CACGTTCTGG 
ATGAATCCTC AGTTTAAGAT CAAGCTGGAG GAGGAAGATG ATGATCCGGA TGATAATGAA 
GTGGGCTGCA GTGTGGTGAT CGGCTTGATT CAGAAGAACC GCCGCAAGAT GAGGAAGGCT 
GGAGAAGACA TGCACACTAT CGGCTATGCC ATCTACGAGG TGCCTTCTCA GTTTCATGGG 
CAAAAGGAGG TTCATCTGGA TAAGAACTAT TTCCTGACTC ACGCTCAGAA AGCTCGATCT 
GAAACCTTTA TCAACCTCAG AGAGGTCAGC ACCCGCTTCA AACTTCCTCC TGGAGAATAT 
CTCATCGTCC CTTCTACATT CGATCCGCAC AAGAACGGGG ATTTTTGTGT GCGCGTGTTC 
TCCGAGAAAC AGTCTGAGAT GCAACAATGT GATGACCCTA TAGAAGCAAA TGTAGATGAT 
GAAATGGTGT CTGAAGAAGA GGTTGATTCT GGCTTTAGGG GCCTGTTTAC TAAACTTGCA 
GGAGATGACA TGGAGATCTC TGCTTCTGAG CTGCGAACGA TTTTCAACAA AATAGTGGCA 
AAGAGAACCG ATATCAAGAC TGATGGTTTC AGTCTGGATA CATGTAGGGT GATGGTCAAC 
CTCATGGATG AAAGTGGAAA TGGAAAACTA GGATTAACTG AGTTTGCTAC ACTTTGGAAA 
AAGATTCAAA AGTATCTGGG CATTTATAAG AAGAATGACA TGGATGGATC CGGCTGTATG 
AGTACACCAG AGATGCGAAT GGCTTTGAAG GAAGCAGGAT TCTCTCTGAA TGACTGCATT 
CACCAGAGTC TGGCTGCACG ATATGGAGAC GCAGACATGA CCATCGACTT TGACAACTTT 
GTGTCCTGTG TGATGCGCCT GGAGATGATG TTCAAAGTGT TCAAGAGGAT GGATATAGAC 
CACAGCGGGT TCATTGAGCT GGACTTCTTT CAGTGGCTGT CATTTGCTAT GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components.
Nature568(7751)259-263(2019 04)
Ma Z,Zhu P,Shi H,Guo L,Zhang Q,Chen Y,Chen S,Zhang Z,Peng J,Chen J

Capn5 Expression in the Healthy and Regenerating Zebrafish Retina.
Investigative ophthalmology & visual science59(8)3643-3654(2018 07)
Coomer CE,Morris AC

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Toxicology and applied pharmacology29631-41(2016 Apr)
Bugel SM,Wehmas LC,La Du JK,Tanguay RL

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capn2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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