ide cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ide
Entrez Gene ID	561390
Full Name	insulin-degrading enzyme
Synonyms	zgc:162603
General protein information	Preferred Names insulin-degrading enzyme Names insulin-degrading enzyme insulysin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169527.3	XP_005169584.1	insulin-degrading enzyme isoform X1
XM_005169528.3	XP_005169585.1	insulin-degrading enzyme isoform X2
XM_005169528.4	XP_005169585.1	insulin-degrading enzyme isoform X2
NM_001089525.1	NP_001082994.1	insulin-degrading enzyme
XM_005169527.4	XP_005169584.1	insulin-degrading enzyme isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E05105g	XP_454175.1
Bos taurus (cattle)	IDE	NP_001069317.1
Danio rerio (zebrafish)	ide	NP_001082994.1
Caenorhabditis elegans (roundworm)	CELE_Y70C5C.1	NP_507226.2
Caenorhabditis elegans (roundworm)	C28F5.4	NP_495575.2
Arabidopsis thaliana (thale cress)	AT3G57470	NP_001118852.1
Magnaporthe oryzae (rice blast fungus)	MGG_13149	XP_003710564.1
Xenopus tropicalis (tropical clawed frog)	ide	XP_002939138.2
Drosophila melanogaster (fruit fly)	Ide	NP_524182.3
Caenorhabditis elegans (roundworm)	C02G6.2	NP_504531.2
Saccharomyces cerevisiae (baker's yeast)	STE23	NP_013493.2
Neurospora crassa	NCU00481	XP_956166.1
Homo sapiens (human)	IDE	NP_004960.2
Pan troglodytes (chimpanzee)	IDE	XP_507922.2
Macaca mulatta (Rhesus monkey)	IDE	XP_001090249.1
Oryza sativa (rice)	Os07g0570300	NP_001060044.1
Schizosaccharomyces pombe (fission yeast)	mug138	NP_593966.1
Canis lupus familiaris (dog)	IDE	XP_534963.3
Mus musculus (house mouse)	Ide	NP_112419.3
Rattus norvegicus (Norway rat)	Ide	NP_037291.1
Gallus gallus (chicken)	IDE	XP_421686.4
Caenorhabditis elegans (roundworm)	F44E7.4	NP_741542.1
Caenorhabditis elegans (roundworm)	C02G6.1	NP_504532.1
Arabidopsis thaliana (thale cress)	AT2G41790	NP_181710.1
Eremothecium gossypii	AGOS_AER053C	NP_984913.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010351	XP_311589.4

Gene Ontology
Bibliography

Related articles in PubMed

Toxic effects of boscalid in adult zebrafish (Danio rerio) on carbohydrate and lipid metabolism.
Qian L, Zhang J, Chen X, Qi S, Wu P, Wang C, Wang C
Environmental pollution (Barking, Essex : 1987)247775-782(2019 Apr)

Analyses of mRNA structure dynamics identify embryonic gene regulatory programs.
Beaudoin JD, Novoa EM, Vejnar CE, Yartseva V, Takacs CM, Kellis M, Giraldez AJ
Nature structural & molecular biology25(8)677-686(2018 08)

Thifluzamide affects lipid metabolism in zebrafish (Danio reio).
Yang Y, Dong F, Liu X, Xu J, Wu X, Qi S, Liu W, Zheng Y
The Science of the total environment6331227-1236(2018 Aug)

Altered glycometabolism in zebrafish exposed to thifluzamide.
Yang Y, Liu W, Li D, Qian L, Fu B, Wang C
Chemosphere18389-96(2017 Sep)

Chronic perfluorooctanesulfonic acid exposure disrupts lipid metabolism in zebrafish.
Cui Y, Lv S, Liu J, Nie S, Chen J, Dong Q, Huang C, Yang D
Human & experimental toxicology36(3)207-217(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ide gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ide cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31317	NM_001089525.1 Latest version!	Danio rerio insulin-degrading enzyme (ide), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa19075	XM_005169527.4 Latest version!	Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa19076	XM_005169528.4 Latest version!	Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa19076	XM_005169528.3	Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa19075	XM_005169527.3	Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ide ( NM_001089525.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082994.1 Danio rerio insulin-degrading enzyme (ide), mRNA.

Price & Availability↑

CloneID	ODa31317
Clone ID Related Accession (Same CDS sequence)	NM_001089525.1
Accession Version	NM_001089525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Danio rerio(zebrafish)
Product	insulin-degrading enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139607.1. On Nov 18, 2007 this sequence version replaced XM_679644.2. ##Evidence-Data-START## Transcript exon combination :: BC139607.1, AB303424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC GTCAATGGTG GTGAAGCTTT TTGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCTGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTCATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG 
TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG 
GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA 
AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GATGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GGTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TTTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCC 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082994.1
CDS	144..3080
Translation	MSDPAVKRVVSDIIRSPEDKREYRGLEFTNGLKAILISDPTTDKSSAALDVHMGSLSDPENISGLAHFCEHMLFLGTEKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLQGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNLMNDAWRLFQLEKATGNPKHPFSKFGTGNKLTLETRPSQQGIDIREELLKFHSTYYSSNLMGLCVLGRETLDELTSMVVKLFGEVENKNVPVPEFPTHPFQEEHLRQFYKVVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIFHMFQYIQKLRTEGPQEWVFQECKDLNTVAFRFKDKERPRGYTSKVAGLLHYYPLEEILAAEYLLEEFRPDLIEMVLDKLRPENVRVAVVSKSFEGQTDRTEEWYGTQYKQEAITDEAIKKWDNADLNGKFKLPMKNEFIPTNFEIYPLEKDSPSAPTLIKDTAMSKVWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTVYGMYLSVKGYNDKQHILLKKIIEKMATFEIDEKRFDIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELRDALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQSALEMMQMLEDTLIEHAHTKPLLPSQLIRYREVQVPDGGWYVYQQRNEVHNNCGIEIYYQTDMQNTHENMLLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGVQGLRFIIQSEKAPHYLESRVEAFLKTMEKSVEEMGDEAFQKHIQALAIRRLDKPKKLAAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEHIMQFYRDLLAIDAPRRHKVSVHVLSREMDSCPLVGEFPAQNDVNLAPAPSLPQPSLVQDMTEFKRSLPLFPLTKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001089525.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin-degrading enzyme (ide), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089525.1

ORF Insert Sequence:

ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC GTCAATGGTG GTGAAGCTTT TTGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCTGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTCATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG 
TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG 
GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA 
AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GATGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GGTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TTTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCC 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ide ( XM_005169527.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169584.1 Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19075
Clone ID Related Accession (Same CDS sequence)	XM_005169527.3 , XM_005169527.4
Accession Version	XM_005169527.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	insulin-degrading enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169527.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG 
TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG 
GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA 
AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169584.1
CDS	333..3329
Translation	MILSTVFGRSIRRVSTLSIRMSDPAVKRVVSDIIRSPEDKREYRGLEFTNGLKAILISDPTTDKSSAALDVHMGSLSDPENISGLAHFCEHMLFLGTEKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLQGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNLMNDAWRLFQLEKATGNPKHPFSKFGTGNKLTLETRPSQQGIDIREELLKFHSTYYSSNLMGLCVLGRETLDELTSMVVKLFGEVENKNVPVPEFPTHPFQEEHLRQFYKVVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIFHMFQYIQKLRTEGPQEWVFQECKDLNTVAFRFKDKERPRGYTSKVAGLLHYYPLEEILAAEYLLEEFRPDLIEMVLDKLRPENVRVAVVSKSFEGQTDRTEEWYGTQYKQEAITDEAIKKWDNADLNGKFKLPMKNEFIPTNFEIYPLEKDSPSAPTLIKDTAMSKVWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTVYGMYLSVKGYNDKQHILLKKIIEKMATFEIDEKRFDIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELRDALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQSALEMMQMLEDTLIEHAHTKPLLPSQLIRYREVQVPDGGWYVYQQRNEVHNNCGIEIYYQTDMQNTHENMLLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGVQGLRFIIQSEKAPHYLESRVEAFLKTMEKSVEEMGDEAFQKHIQALAIRRLDKPKKLAAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEHIMQFYRDLLAIDAPRRHKVSVHVLSREMDSCPLVGEFPAQNDVNLAPAPSLPQPSLVQDMTEFKRSLPLFPLTKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_005169527.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169527.3

ORF Insert Sequence:

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG 
TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG 
GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA 
AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ide ( XM_005169527.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169584.1 Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19075
Clone ID Related Accession (Same CDS sequence)	XM_005169527.3 , XM_005169527.4
Accession Version	XM_005169527.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	insulin-degrading enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169527.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG 
TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG 
GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA 
AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169584.1
CDS	333..3329
Translation	MILSTVFGRSIRRVSTLSIRMSDPAVKRVVSDIIRSPEDKREYRGLEFTNGLKAILISDPTTDKSSAALDVHMGSLSDPENISGLAHFCEHMLFLGTEKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLQGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNLMNDAWRLFQLEKATGNPKHPFSKFGTGNKLTLETRPSQQGIDIREELLKFHSTYYSSNLMGLCVLGRETLDELTSMVVKLFGEVENKNVPVPEFPTHPFQEEHLRQFYKVVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIFHMFQYIQKLRTEGPQEWVFQECKDLNTVAFRFKDKERPRGYTSKVAGLLHYYPLEEILAAEYLLEEFRPDLIEMVLDKLRPENVRVAVVSKSFEGQTDRTEEWYGTQYKQEAITDEAIKKWDNADLNGKFKLPMKNEFIPTNFEIYPLEKDSPSAPTLIKDTAMSKVWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTVYGMYLSVKGYNDKQHILLKKIIEKMATFEIDEKRFDIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELRDALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQSALEMMQMLEDTLIEHAHTKPLLPSQLIRYREVQVPDGGWYVYQQRNEVHNNCGIEIYYQTDMQNTHENMLLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGVQGLRFIIQSEKAPHYLESRVEAFLKTMEKSVEEMGDEAFQKHIQALAIRRLDKPKKLAAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEHIMQFYRDLLAIDAPRRHKVSVHVLSREMDSCPLVGEFPAQNDVNLAPAPSLPQPSLVQDMTEFKRSLPLFPLTKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_005169527.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin-degrading enzyme (ide), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169527.4

ORF Insert Sequence:

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGCCCG 
TTTGCATACG TGGACCCGTT GCATTGTAAC ATGGCGTATT TGTACCTTGA GCTGCTTAAG 
GATTCGCTGA ATGAGTATGC ATATGCAGCC GAGTTAGCCG GCCTGAGCTA TGACCTTCAA 
AATACCGTCT ATGGGATGTA TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ide ( XM_005169528.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169585.1 Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa19076
Clone ID Related Accession (Same CDS sequence)	XM_005169528.3 , XM_005169528.4
Accession Version	XM_005169528.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	insulin-degrading enzyme isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC 
TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG 
GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC 
TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169585.1
CDS	333..3329
Translation	MILSTVFGRSIRRVSTLSIRMSDPAVKRVVSDIIRSPEDKREYRGLEFTNGLKAILISDPTTDKSSAALDVHMGSLSDPENISGLAHFCEHMLFLGTEKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLQGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNLMNDAWRLFQLEKATGNPKHPFSKFGTGNKLTLETRPSQQGIDIREELLKFHSTYYSSNLMGLCVLGRETLDELTSMVVKLFGEVENKNVPVPEFPTHPFQEEHLRQFYKVVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIFHMFQYIQKLRTEGPQEWVFQECKDLNTVAFRFKDKERPRGYTSKVAGLLHYYPLEEILAAEYLLEEFRPDLIEMVLDKLRPENVRVAVVSKSFEGQTDRTEEWYGTQYKQEAITDEAIKKWDNADLNGKFKLPMKNEFIPTNFEIYPLEKDSPSAPTLIKDTAMSKVWFKQDDKFFLPKACLNFEFFSRYLYADPVHCNMTYLFLRLLKDDLREYTYAARLAGLVYGIASGMNAILLSVKGYNDKQHILLKKIIEKMATFEIDEKRFDIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELRDALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQSALEMMQMLEDTLIEHAHTKPLLPSQLIRYREVQVPDGGWYVYQQRNEVHNNCGIEIYYQTDMQNTHENMLLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGVQGLRFIIQSEKAPHYLESRVEAFLKTMEKSVEEMGDEAFQKHIQALAIRRLDKPKKLAAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEHIMQFYRDLLAIDAPRRHKVSVHVLSREMDSCPLVGEFPAQNDVNLAPAPSLPQPSLVQDMTEFKRSLPLFPLTKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_005169528.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169528.3

ORF Insert Sequence:

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC 
TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG 
GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC 
TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ide ( XM_005169528.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169585.1 Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.

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CloneID	ODa19076
Clone ID Related Accession (Same CDS sequence)	XM_005169528.3 , XM_005169528.4
Accession Version	XM_005169528.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	insulin-degrading enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169528.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC 
TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG 
GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC 
TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169585.1
CDS	333..3329
Translation	MILSTVFGRSIRRVSTLSIRMSDPAVKRVVSDIIRSPEDKREYRGLEFTNGLKAILISDPTTDKSSAALDVHMGSLSDPENISGLAHFCEHMLFLGTEKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLQGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNLMNDAWRLFQLEKATGNPKHPFSKFGTGNKLTLETRPSQQGIDIREELLKFHSTYYSSNLMGLCVLGRETLDELTSMVVKLFGEVENKNVPVPEFPTHPFQEEHLRQFYKVVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIFHMFQYIQKLRTEGPQEWVFQECKDLNTVAFRFKDKERPRGYTSKVAGLLHYYPLEEILAAEYLLEEFRPDLIEMVLDKLRPENVRVAVVSKSFEGQTDRTEEWYGTQYKQEAITDEAIKKWDNADLNGKFKLPMKNEFIPTNFEIYPLEKDSPSAPTLIKDTAMSKVWFKQDDKFFLPKACLNFEFFSRYLYADPVHCNMTYLFLRLLKDDLREYTYAARLAGLVYGIASGMNAILLSVKGYNDKQHILLKKIIEKMATFEIDEKRFDIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELRDALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQSALEMMQMLEDTLIEHAHTKPLLPSQLIRYREVQVPDGGWYVYQQRNEVHNNCGIEIYYQTDMQNTHENMLLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGVQGLRFIIQSEKAPHYLESRVEAFLKTMEKSVEEMGDEAFQKHIQALAIRRLDKPKKLAAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEHIMQFYRDLLAIDAPRRHKVSVHVLSREMDSCPLVGEFPAQNDVNLAPAPSLPQPSLVQDMTEFKRSLPLFPLTKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_005169528.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin-degrading enzyme (ide), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169528.4

ORF Insert Sequence:

ATGATATTGT CCACGGTTTT CGGTCGAAGT ATTAGACGTG TGTCGACTCT GTCCATCAGA 
ATGAGTGATC CGGCTGTCAA GAGGGTGGTG AGTGATATCA TTCGCTCTCC AGAAGACAAA 
CGGGAGTATC GAGGACTCGA GTTTACAAAT GGTCTGAAAG CCATTCTCAT CAGTGACCCC 
ACCACTGACA AATCATCAGC TGCCCTGGAC GTCCACATGG GCTCTTTGTC AGATCCTGAG 
AACATTTCAG GCCTGGCCCA CTTCTGCGAG CACATGCTGT TTCTGGGCAC TGAGAAGTAT 
CCCAAAGAGA ACGAGTACAG CCAGTTTTTG AGTGAGCATG CCGGCAGCTC GAACGCCTTC 
ACCAGCGGAG AGCACACCAA TTACTACTTT GATGTATCCC ATGAGCATCT GCAAGGGGCG 
TTGGACAGAT TTGCCCAGTT CTTCCTCTGT CCCCTGTTTG ATGAGAGCTG TAAGGACCGA 
GAGGTGAATG CAGTGGACTC TGAACATGAG AAGAACCTCA TGAATGACGC ATGGCGGTTG 
TTTCAGTTGG AGAAAGCCAC TGGGAACCCA AAACACCCTT TTAGCAAATT TGGAACCGGC 
AACAAGTTAA CCCTTGAGAC CAGGCCATCT CAACAGGGCA TTGATATCCG CGAGGAGCTG 
CTAAAGTTTC ACTCTACCTA TTATTCCTCC AACCTGATGG GATTGTGTGT GCTGGGCAGA 
GAGACGCTGG ATGAATTGAC ATCAATGGTG GTGAAGCTTT TCGGAGAGGT GGAGAACAAG 
AATGTCCCTG TCCCAGAATT CCCTACACAT CCTTTCCAGG AGGAACACCT GAGGCAATTC 
TATAAAGTGG TGCCAATCAA GGACATCAGA AACTTGTATG TGACCTTCCC TATACCAGAC 
CTGCAGAAGT ACTACAAATC AAATCCGGGT CATTATCTTG GCCACCTGAT TGGACACGAG 
GGTCCAGGCA GTCTTTTGTC TGAGCTCAAA TCTAAAGGAT GGGTGAACAC TCTAGTTGGA 
GGCCAGAAAG AAGGAGCCAG AGGATTCATG TTCTTCATTA TTAATGTGGA TTTGACAGAA 
GAGGGGCTGT TGCATGTCGA GGATATCATC TTCCACATGT TTCAGTACAT TCAGAAGCTG 
CGGACGGAGG GCCCTCAGGA GTGGGTCTTT CAGGAGTGTA AGGATTTGAA CACCGTGGCT 
TTCCGATTTA AGGATAAGGA ACGTCCTCGT GGATACACGT CTAAAGTGGC TGGACTTCTG 
CATTACTATC CGCTGGAAGA AATTTTGGCT GCCGAATACT TGCTGGAAGA GTTCAGGCCA 
GACCTCATTG AGATGGTGCT TGATAAACTG AGACCAGAGA ATGTCAGGGT TGCAGTTGTC 
TCCAAATCAT TTGAAGGACA GACTGATCGG ACTGAGGAGT GGTACGGCAC ACAGTATAAA 
CAAGAAGCCA TTACTGATGA AGCTATTAAG AAATGGGACA ATGCAGACCT CAACGGAAAG 
TTTAAACTGC CAATGAAGAA TGAATTTATC CCCACAAACT TTGAGATTTA CCCTCTAGAG 
AAGGATTCCC CATCTGCCCC CACTTTAATA AAGGACACTG CAATGAGCAA AGTTTGGTTC 
AAACAAGATG ACAAGTTTTT CCTGCCTAAA GCTTGTTTAA ACTTTGAGTT TTTCAGTCGC 
TACCTTTATG CAGACCCAGT GCACTGCAAC ATGACTTACC TGTTTCTCAG GTTGTTGAAG 
GATGATTTGA GAGAGTATAC ATATGCAGCC CGCCTGGCAG GGTTGGTCTA TGGCATAGCC 
TCAGGAATGA ATGCCATCCT TCTGTCAGTC AAGGGTTATA ATGACAAGCA ACACATCCTG 
CTGAAGAAGA TCATTGAGAA AATGGCCACG TTTGAGATCG ATGAGAAGCG GTTTGATATC 
ATTAAGGAAG CGTACATGAG GTCTTTGAAT AACTTCCGCG CTGAGCAGCC TCACCAGCAC 
GCCATGTATT ACCTCCGTCT GCTCATGACT GAAGTGGCCT GGACCAAAGA TGAGCTCAGA 
GACGCTCTGG ATGATGTGAC GTTACCTCGT CTGAAGGCGT TCATACCGCA GCTGTTGTCA 
CGGTTACACA TTGAAGCACT TCTGCATGGC AACATAACCA AACAGTCAGC TCTGGAAATG 
ATGCAAATGC TGGAAGACAC TCTCATTGAG CATGCTCACA CTAAACCCCT TCTACCGAGT 
CAGCTGATTC GCTACAGAGA GGTGCAGGTT CCTGATGGTG GTTGGTATGT ATACCAGCAG 
AGGAACGAGG TTCATAATAA CTGTGGGATC GAGATTTACT ATCAGACTGA CATGCAGAAC 
ACCCATGAGA ACATGCTGCT GGAGCTCTTC TGTCAGATCA TCTCTGAGCC ATGCTTCAAC 
ACGCTACGCA CCAAAGAACA GCTGGGTTAT ATTGTGTTCA GTGGGCCTCG CAGGGCCAAT 
GGGGTGCAGG GTCTGCGCTT CATCATCCAG TCTGAAAAAG CTCCCCACTA CCTGGAGTCT 
CGTGTAGAGG CTTTCCTGAA GACCATGGAG AAGAGCGTGG AGGAGATGGG AGACGAGGCC 
TTTCAGAAAC ACATCCAGGC ACTGGCCATT CGCCGTCTTG ACAAACCCAA GAAGCTGGCG 
GCCGAGTGTG CAAAGTACTG GGGAGAGATC ATCTCACAAC AGTACAACTT CGACAGGGAT 
AATATTGAAG TGGCTTACCT GAAGACACTG ACAAAGGAAC ACATTATGCA GTTTTACAGG 
GACTTGTTGG CCATTGATGC CCCCAGAAGA CATAAAGTGT CTGTGCATGT GCTGTCTAGA 
GAGATGGACT CTTGTCCACT GGTCGGAGAG TTTCCTGCTC AAAATGATGT CAATCTGGCT 
CCTGCTCCAT CACTTCCTCA GCCATCATTG GTTCAGGATA TGACTGAGTT CAAGAGGAGT 
CTGCCGCTGT TTCCCCTCAC CAAACCTCAC ATCAACTTCA TGGCTGCCAA ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Toxic effects of boscalid in adult zebrafish (Danio rerio) on carbohydrate and lipid metabolism.
Environmental pollution (Barking, Essex : 1987)247775-782(2019 Apr)
Qian L,Zhang J,Chen X,Qi S,Wu P,Wang C,Wang C

Analyses of mRNA structure dynamics identify embryonic gene regulatory programs.
Nature structural & molecular biology25(8)677-686(2018 08)
Beaudoin JD,Novoa EM,Vejnar CE,Yartseva V,Takacs CM,Kellis M,Giraldez AJ

Thifluzamide affects lipid metabolism in zebrafish (Danio reio).
The Science of the total environment6331227-1236(2018 Aug)
Yang Y,Dong F,Liu X,Xu J,Wu X,Qi S,Liu W,Zheng Y

Altered glycometabolism in zebrafish exposed to thifluzamide.
Chemosphere18389-96(2017 Sep)
Yang Y,Liu W,Li D,Qian L,Fu B,Wang C

Chronic perfluorooctanesulfonic acid exposure disrupts lipid metabolism in zebrafish.
Human & experimental toxicology36(3)207-217(2017 Mar)
Cui Y,Lv S,Liu J,Nie S,Chen J,Dong Q,Huang C,Yang D

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ide cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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