IDE cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	IDE
Entrez Gene ID	477768
Full Name	insulin degrading enzyme
General protein information	Preferred Names insulin degrading enzyme Names insulin-degrading enzyme insulin-degrading enzyme isoform 15a insulin-degrading protein enzyme 15b
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014109026.1	XP_013964501.1	LOW QUALITY PROTEIN: insulin-degrading enzyme
NM_001314100.1	NP_001301029.1	insulin-degrading enzyme

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E05105g	XP_454175.1
Bos taurus (cattle)	IDE	NP_001069317.1
Danio rerio (zebrafish)	ide	NP_001082994.1
Caenorhabditis elegans (roundworm)	CELE_Y70C5C.1	NP_507226.2
Caenorhabditis elegans (roundworm)	C28F5.4	NP_495575.2
Arabidopsis thaliana (thale cress)	AT3G57470	NP_001118852.1
Magnaporthe oryzae (rice blast fungus)	MGG_13149	XP_003710564.1
Xenopus tropicalis (tropical clawed frog)	ide	XP_002939138.2
Drosophila melanogaster (fruit fly)	Ide	NP_524182.3
Caenorhabditis elegans (roundworm)	C02G6.2	NP_504531.2
Saccharomyces cerevisiae (baker's yeast)	STE23	NP_013493.2
Neurospora crassa	NCU00481	XP_956166.1
Homo sapiens (human)	IDE	NP_004960.2
Pan troglodytes (chimpanzee)	IDE	XP_507922.2
Macaca mulatta (Rhesus monkey)	IDE	XP_001090249.1
Oryza sativa (rice)	Os07g0570300	NP_001060044.1
Schizosaccharomyces pombe (fission yeast)	mug138	NP_593966.1
Canis lupus familiaris (dog)	IDE	XP_534963.3
Mus musculus (house mouse)	Ide	NP_112419.3
Rattus norvegicus (Norway rat)	Ide	NP_037291.1
Gallus gallus (chicken)	IDE	XP_421686.4
Caenorhabditis elegans (roundworm)	F44E7.4	NP_741542.1
Caenorhabditis elegans (roundworm)	C02G6.1	NP_504532.1
Arabidopsis thaliana (thale cress)	AT2G41790	NP_181710.1
Eremothecium gossypii	AGOS_AER053C	NP_984913.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010351	XP_311589.4

Gene Ontology
Bibliography

Related articles in PubMed

Cloning, sequencing and expression in the dog of the main amyloid precursor protein isoforms and some of the enzymes related with their processing.
Sarasa L, Gallego C, Monleón I, Olvera A, Canudas J, Montañés M, Pesini P, Sarasa M
Neuroscience171(4)1091-101(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe140217	NM_001314100.1 Latest version!	Canis lupus familiaris insulin degrading enzyme (IDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe43986	XM_014109026.1 Latest version!	Canis lupus familiaris insulin-degrading enzyme (IDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IDE ( NM_001314100.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001301029.1 Canis lupus familiaris insulin degrading enzyme (IDE), mRNA.

Price & Availability↑

CloneID	OCe140217
Clone ID Related Accession (Same CDS sequence)	NM_001314100.1
Accession Version	NM_001314100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Canis lupus familiaris(dog)
Product	insulin-degrading enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KF386029.1. ##Evidence-Data-START## Transcript exon combination :: KF386029.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGCGGTACC CGCTAGCGTG GCTGCTGCAC TCCGCGCGGC CCAGCACCTT CCGCTCCGTC 
CTCGGCGCCC GCCTGCCGCT CCCGGAGCGC CTCTGTGGTT TCCAAGAGAA GAGTTATAGC 
AAAATGAATA ATCCAGCTAT CAAGAGACTA GGAAATCACA TTATCAAATC CCCTGAAGAC 
AAGAGAGAAT ACCGTGGGCT AGAACTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCGACCACAG ATAAGTCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACCAGTG GAGAACATAC CAATTACTAT TTTGACGTTT CCCACGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCTTGGAGA 
CTCTTTCAGT TGGAAAAAGC TACGGGGAAT CCCAAACACC CCTTCAGCAA ATTTGGGACA 
GGTAACAAAT ACACTTTAGA GACTCGACCA AACCAAGAAG GCATTGATGT CAGGCAAGAG 
CTACTGAAAT TCCACTCTAC TTATTATTCA TCCAACTTAA TGGCTATTTG TGTGTTAGGT 
CGAGAATCCT TAGACGACTT AACTAATCTG GTGGTAAAGC TATTTTCTGA AGTTGAGAAC 
AAAAATGTCC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGCCATTATC TTGGTCATCT AATTGGTCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGACAGA AGGAGGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACACGT TGAAGATATA ATTTTGCACA TGTTCCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCATTTAGGT TTAAAGATGA AGAGAGGCCA CGGGGCTATA CCTCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAGT ATTTGCTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAGT CTTTTGAAGG AAAGACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTGTACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCAACGAA GAATGAATTT ATTCCTACAA ATTTTGAGAT TTTATCATTA 
GAAAAAGAAG CAACACCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCA AAGGCTTGTC TGAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAGCA GCCTCACCAA 
CACGCCATGT ACTACCTCCG TTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCC TGGATGATGT TACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATCGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCCCTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACAAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAGAC AGACATGCAG 
AGCACCTCAG AGAATATGTT TCTAGAGCTC TTCTGTCAGA TCATCTCTGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGCTGGGC TATATTGTCT TCAGCGGGCC GCGTCGAGCC 
AACGGCATAC AGGGCTTACG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGTAGAGTGG AAGCTTTCCT AATCACCATG GAGAAGTCCA TAGAGGACAT GACAGAAGAG 
GCTTTTCAAA AACACATTCA AGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAACTG 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAA ATCATCTCCC AGCAATACAA TTTTGACAGA 
GACAATACAG AAGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCCCCTAGG AGACATAAGG TATCAGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTCGGA GAGTTCCCGT GTCAAAATGA TATAAATCTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA ATTCAAGCGA 
GGTTTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001301029.1
CDS	1..3060
Translation	MRYPLAWLLHSARPSTFRSVLGARLPLPERLCGFQEKSYSKMNNPAIKRLGNHIIKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDEERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAVPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSRYIYADPLHCNMTYLFIRLLKDDLKEYTYAARLSGLSYGIASGMNAILLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	NM_001314100.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris insulin degrading enzyme (IDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314100.1

ORF Insert Sequence:

ATGCGGTACC CGCTAGCGTG GCTGCTGCAC TCCGCGCGGC CCAGCACCTT CCGCTCCGTC 
CTCGGCGCCC GCCTGCCGCT CCCGGAGCGC CTCTGTGGTT TCCAAGAGAA GAGTTATAGC 
AAAATGAATA ATCCAGCTAT CAAGAGACTA GGAAATCACA TTATCAAATC CCCTGAAGAC 
AAGAGAGAAT ACCGTGGGCT AGAACTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCGACCACAG ATAAGTCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACCAGTG GAGAACATAC CAATTACTAT TTTGACGTTT CCCACGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCTTGGAGA 
CTCTTTCAGT TGGAAAAAGC TACGGGGAAT CCCAAACACC CCTTCAGCAA ATTTGGGACA 
GGTAACAAAT ACACTTTAGA GACTCGACCA AACCAAGAAG GCATTGATGT CAGGCAAGAG 
CTACTGAAAT TCCACTCTAC TTATTATTCA TCCAACTTAA TGGCTATTTG TGTGTTAGGT 
CGAGAATCCT TAGACGACTT AACTAATCTG GTGGTAAAGC TATTTTCTGA AGTTGAGAAC 
AAAAATGTCC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGCCATTATC TTGGTCATCT AATTGGTCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGACAGA AGGAGGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACACGT TGAAGATATA ATTTTGCACA TGTTCCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCATTTAGGT TTAAAGATGA AGAGAGGCCA CGGGGCTATA CCTCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAGT ATTTGCTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAGT CTTTTGAAGG AAAGACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTGTACCAGA CGAAGTCATT AAGAAATGGC AAAATGCTGA CCTGAATGGG 
AAATTTAAAC TTCCAACGAA GAATGAATTT ATTCCTACAA ATTTTGAGAT TTTATCATTA 
GAAAAAGAAG CAACACCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCA AAGGCTTGTC TGAACTTTGA ATTTTTCAGT 
CGCTACATTT ATGCTGATCC TCTCCATTGC AACATGACAT ACCTGTTTAT CAGGTTATTG 
AAGGATGATT TAAAAGAGTA TACATATGCA GCACGCCTCT CAGGTTTGAG CTATGGCATT 
GCATCAGGAA TGAATGCAAT ACTTCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAGCA GCCTCACCAA 
CACGCCATGT ACTACCTCCG TTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCC TGGATGATGT TACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATCGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCCCTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACAAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAGAC AGACATGCAG 
AGCACCTCAG AGAATATGTT TCTAGAGCTC TTCTGTCAGA TCATCTCTGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGCTGGGC TATATTGTCT TCAGCGGGCC GCGTCGAGCC 
AACGGCATAC AGGGCTTACG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGTAGAGTGG AAGCTTTCCT AATCACCATG GAGAAGTCCA TAGAGGACAT GACAGAAGAG 
GCTTTTCAAA AACACATTCA AGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAACTG 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAA ATCATCTCCC AGCAATACAA TTTTGACAGA 
GACAATACAG AAGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCCCCTAGG AGACATAAGG TATCAGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTCGGA GAGTTCCCGT GTCAAAATGA TATAAATCTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA ATTCAAGCGA 
GGTTTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDE ( XM_014109026.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964501.1 Canis lupus familiaris insulin-degrading enzyme (IDE), mRNA.

Price & Availability↑

CloneID	OCe43986
Clone ID Related Accession (Same CDS sequence)	XM_014109026.1
Accession Version	XM_014109026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	LOW QUALITY PROTEIN: insulin-degrading enzyme
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCGGTACC CGCTAGCGTG GCTGCTGCAC TCCGCGCGGC CCAGCACCTT CCGCTCCGTC 
CTCGGCGCCC GCCTGCCGCT CCCGGAGCGC CTCTGTGGTT TCCAAGAGAA GAGTTATAGC 
AAAATGAATA ATCCAGCTAT CAAGAGACTA GGAAATCACA TTATCAAATC CCCTGAAGAC 
AAGAGAGAAT ACCGTGGGCT AGAACTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCGACCACAG ATAAGTCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACCAGTG GAGAACATAC CAATTACTAT TTTGACGTTT CCCACGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCTTGGAGA 
CTCTTTCAGT TGGAAAAAGC TACGGGGAAT CCCAAACACC CCTTCAGCAA ATTTGGGACA 
GGTAACAAAT ACACTTTAGA GACTCGACCA AACCAAGAAG GCATTGATGT CAGGCAAGAG 
CTACTGAAAT TCCACTCTAC TTATTATTCA TCCAACTTAA TGGCTATTTG TGTGTTAGGT 
CGAGAATCCT TAGACGACTT AACTAATCTG GTGGTAAAGC TATTTTCTGA AGTTGAGAAC 
AAAAATGTCC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGCCATTATC TTGGTCATCT AATTGGTCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGACAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACACGT TGAAGATATA ATTTTGCACA TGTTCCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCATTTAGGT TTAAAGATAA AGAGAGGCCA CGGGGCTATA CCTCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAGT ATTTGCTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAGT CTTTTGAAGG AAAGACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTGTACCAGA CGAAGTCATT AAGAAATGGC AACATGCTGA CCTGATGGAA 
AAATTTAACT TTCCACGAGC GCATGATTTT ATTCCTACAA TTGCTGAGAT TTTATCATTA 
GAAAAAGAAG CACACCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCA AAGGCTTGTC TGAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGACCTC 
CAAAATACCA TCTATGGGAT GTATCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAGCA GCCTCACCAA 
CACGCCATGT ACTACCTCCG TTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCC TGGATGATGT TACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATCGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCCCTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACAAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAGAC AGACATGCAG 
AGCACCTCAG AGAATATGTT TCTAGAGCTC TTCTGTCAGA TCATCTCTGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGCTGGGC TATATTGTCT TCAGCGGGCC GCGTCGAGCC 
AACGGCATAC AGGGCTTACG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGTAGAGTGG AAGCTTTCCT AATCACCATG GAGAAGTCCA TAGAGGACAT GACAGAAGAG 
GCTTTTCAAA AACACATTCA AGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAACTG 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAA ATCATCTCCC AGCAATACAA TTTTGACAGA 
GACAATACAG AAGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCCCCTAGG AGACATAAGG TATCAGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTCGGA GAGTTCCCGT GTCAAAATGA TATAAATCTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA ATTCAAGCGA 
GGTTTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964501.1
CDS	82..3141
Translation	MRYPLAWLLHSARPSTFRSVLGARLPLPERLCGFQEKSYSKMNNPAIKRLGNHIIKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAVPDEVIKKWQHADLMEKFNFPRAHDFIPTIAEILSLEKEAHPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Target ORF information:

RefSeq Version	XM_014109026.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris insulin-degrading enzyme (IDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109026.1

ORF Insert Sequence:

ATGCGGTACC CGCTAGCGTG GCTGCTGCAC TCCGCGCGGC CCAGCACCTT CCGCTCCGTC 
CTCGGCGCCC GCCTGCCGCT CCCGGAGCGC CTCTGTGGTT TCCAAGAGAA GAGTTATAGC 
AAAATGAATA ATCCAGCTAT CAAGAGACTA GGAAATCACA TTATCAAATC CCCTGAAGAC 
AAGAGAGAAT ACCGTGGGCT AGAACTGGCC AATGGTATCA AAGTACTTCT TATCAGTGAT 
CCGACCACAG ATAAGTCATC AGCAGCACTT GATGTACACA TAGGTTCATT GTCAGATCCT 
CCAAATATTG CTGGCTTAAG TCATTTTTGT GAACATATGC TATTTTTGGG AACAAAGAAA 
TACCCTAAAG AAAATGAATA CAGCCAGTTT CTCAGTGAGC ATGCAGGAAG TTCAAATGCC 
TTTACCAGTG GAGAACATAC CAATTACTAT TTTGACGTTT CCCACGAACA CCTAGAAGGT 
GCCCTAGACA GGTTTGCACA GTTTTTCCTG TGCCCCTTGT TTGATGAGAG TTGCAAAGAC 
AGAGAGGTGA ATGCAGTTGA TTCAGAACAT GAGAAGAATG TGATGAACGA TGCTTGGAGA 
CTCTTTCAGT TGGAAAAAGC TACGGGGAAT CCCAAACACC CCTTCAGCAA ATTTGGGACA 
GGTAACAAAT ACACTTTAGA GACTCGACCA AACCAAGAAG GCATTGATGT CAGGCAAGAG 
CTACTGAAAT TCCACTCTAC TTATTATTCA TCCAACTTAA TGGCTATTTG TGTGTTAGGT 
CGAGAATCCT TAGACGACTT AACTAATCTG GTGGTAAAGC TATTTTCTGA AGTTGAGAAC 
AAAAATGTCC CATTGCCAGA ATTTCCTGAA CACCCTTTCC AAGAAGAACA TCTTAAACAA 
CTTTACAAAA TAGTACCCAT TAAGGATATT AGGAATCTTT ATGTGACATT TCCCATACCT 
GACCTTCAGA AATACTACAA ATCAAATCCT GGCCATTATC TTGGTCATCT AATTGGTCAT 
GAAGGTCCTG GAAGTCTGTT ATCAGAACTT AAATCAAAGG GCTGGGTAAA TACTCTTGTT 
GGTGGACAGA AGGAAGGAGC CCGAGGTTTT ATGTTTTTTA TCATTAATGT GGACTTGACT 
GAGGAAGGAT TATTACACGT TGAAGATATA ATTTTGCACA TGTTCCAATA CATTCAGAAG 
TTACGTGCAG AAGGACCTCA AGAATGGGTT TTCCAAGAGT GCAAGGACTT GAATGCTGTT 
GCATTTAGGT TTAAAGATAA AGAGAGGCCA CGGGGCTATA CCTCTAAGAT TGCAGGAATA 
TTGCATTATT ATCCCCTAGA AGAAGTGCTC ACAGCTGAGT ATTTGCTGGA AGAATTTAGA 
CCTGACTTAA TAGAGATGGT TCTCGATAAA CTCAGACCAG AAAATGTCCG GGTTGCAATA 
GTTTCCAAGT CTTTTGAAGG AAAGACTGAT CGCACAGAAG AGTGGTATGG AACCCAGTAC 
AAGCAAGAAG CTGTACCAGA CGAAGTCATT AAGAAATGGC AACATGCTGA CCTGATGGAA 
AAATTTAACT TTCCACGAGC GCATGATTTT ATTCCTACAA TTGCTGAGAT TTTATCATTA 
GAAAAAGAAG CACACCCATA CCCTTCTCTT ATTAAGGATA CAGCTATGAG CAAACTCTGG 
TTCAAACAAG ATGATAAGTT TTTCTTGCCA AAGGCTTGTC TGAACTTTGA ATTTTTCAGC 
CCATTTGCTT ATGTGGACCC CTTGCACTGT AACATGGCCT ATTTGTACCT TGAGCTCCTC 
AAAGACTCAC TCAACGAGTA TGCATATGCA GCAGAGCTAG CAGGCTTGAG CTATGACCTC 
CAAAATACCA TCTATGGGAT GTATCTCTCG GTGAAAGGTT ACAATGACAA GCAGCCAATT 
TTGCTAAAGA AGATCATTGA GAAAATGGCT ACCTTTGAGA TTGATGAAAA AAGATTTGAA 
ATTATCAAAG AGGCATATAT GCGATCTCTT AACAATTTCC GAGCTGAGCA GCCTCACCAA 
CACGCCATGT ACTACCTCCG TTTGCTGATG ACTGAAGTGG CCTGGACTAA AGATGAATTA 
AAAGAAGCCC TGGATGATGT TACCCTTCCT CGCCTTAAGG CCTTCATACC TCAGCTCCTG 
TCACGGCTGC ACATCGAAGC CCTTCTCCAT GGAAACATAA CAAAGCAGGC TGCCCTAGGA 
ATTATGCAGA TGGTTGAAGA CACCCTCATT GAACATGCTC ATACAAAACC TCTCCTTCCA 
AGTCAGCTGG TTCGGTATAG AGAAGTTCAG CTCCCTGACA GAGGATGGTT TGTGTATCAG 
CAGAGGAATG AAGTTCACAA TAACTGTGGC ATCGAGATAT ACTACCAGAC AGACATGCAG 
AGCACCTCAG AGAATATGTT TCTAGAGCTC TTCTGTCAGA TCATCTCTGA ACCTTGCTTC 
AACACCCTGC GCACCAAGGA GCAGCTGGGC TATATTGTCT TCAGCGGGCC GCGTCGAGCC 
AACGGCATAC AGGGCTTACG ATTCATCATC CAGTCAGAAA AGCCACCTCA CTACCTAGAA 
AGTAGAGTGG AAGCTTTCCT AATCACCATG GAGAAGTCCA TAGAGGACAT GACAGAAGAG 
GCTTTTCAAA AACACATTCA AGCATTAGCA ATTCGTCGAC TAGACAAACC AAAGAAACTG 
TCTGCAGAGT GTGCTAAATA TTGGGGGGAA ATCATCTCCC AGCAATACAA TTTTGACAGA 
GACAATACAG AAGTTGCATA TTTAAAGACA CTTACCAAGG AAGATATCAT CAAATTCTAC 
AAGGAAATGT TGGCAGTAGA TGCCCCTAGG AGACATAAGG TATCAGTCCA TGTTCTTGCC 
AGAGAAATGG ATTCTTGTCC TGTGGTCGGA GAGTTCCCGT GTCAAAATGA TATAAATCTG 
TCACAAGCAC CAGCCTTGCC ACAACCTGAA GTGATTCAGA ACATGACTGA ATTCAAGCGA 
GGTTTGCCAC TGTTTCCCCT TGTGAAACCA CATATTAACT TCATGGCTGC AAAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cloning, sequencing and expression in the dog of the main amyloid precursor protein isoforms and some of the enzymes related with their processing.
Neuroscience171(4)1091-101(2010 Dec)
Sarasa L,Gallego C,Monleón I,Olvera A,Canudas J,Montañés M,Pesini P,Sarasa M

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IDE cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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