mhc1zca cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mhc1zca
Entrez Gene ID	556341
Full Name	major histocompatibility complex class I ZCA
Synonyms	dZ63M10.1,si:ch211-287j19.5,zgc:111893,zgc:136614
General protein information	Preferred Names major histocompatibility complex class I ZCA Names uncharacterized protein LOC556341 MHC class I antigen ZCA dZ63M10.1 (novel MHC class I antigen)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083545.1	NP_001077014.1	uncharacterized protein LOC556341 precursor
XM_017354912.1	XP_017210401.1	uncharacterized protein LOC556341 isoform X1
XM_021474930.1	XP_021330605.1	uncharacterized protein LOC556341 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gm8909	NP_001074501.2
Mus musculus (house mouse)	H2-Bl	NP_032225.2
Mus musculus (house mouse)	H2-Q2	NP_034522.2
Rattus norvegicus (Norway rat)	RT1-A1	NP_001008827.1
Rattus norvegicus (Norway rat)	RT1-CE2	NP_001008840.1
Rattus norvegicus (Norway rat)	RT1-CE5	NP_001008843.1
Rattus norvegicus (Norway rat)	RT1-A2	NP_001008829.1
Gallus gallus (chicken)	MICA	XP_415354.4
Gallus gallus (chicken)	MR1	XP_004945738.1
Gallus gallus (chicken)	LOC417083	XP_003642294.2
Danio rerio (zebrafish)	zgc:136614	NP_001077014.1
Gallus gallus (chicken)	LOC100858733	XP_003643847.2
Gallus gallus (chicken)	LOC100858581	XP_003643753.2
Rattus norvegicus (Norway rat)	LOC100364500	XP_006255953.1
Danio rerio (zebrafish)	LOC100536229	XP_003201722.1
Macaca mulatta (Rhesus monkey)	MAMU-A3	NP_001165309.1
Mus musculus (house mouse)	H2-D1	NP_034510.3
Mus musculus (house mouse)	Gm10499	XP_006525296.1
Mus musculus (house mouse)	H2-Q4	NP_001137161.1
Mus musculus (house mouse)	H2-Q7	NP_034524.3
Rattus norvegicus (Norway rat)	RT1-CE7	NP_001008845.1
Rattus norvegicus (Norway rat)	RT1-CE1	NP_001008832.1
Rattus norvegicus (Norway rat)	RT1-CE10	NP_001008833.1
Gallus gallus (chicken)	BF2	NP_001026509.1
Gallus gallus (chicken)	LOC769716	XP_001233005.3
Gallus gallus (chicken)	YF6	XP_003643784.1
Danio rerio (zebrafish)	LOC563036	XP_001919291.4
Gallus gallus (chicken)	LOC101752075	XP_004950216.1
Gallus gallus (chicken)	LOC100859374	XP_003643261.1
Gallus gallus (chicken)	LOC100859408	XP_003643262.1
Gallus gallus (chicken)	LOC100859100	XP_003642292.2
Mus musculus (house mouse)	H2-Q1	NP_034520.2
Rattus norvegicus (Norway rat)	RT1-CE16	NP_001008839.1
Rattus norvegicus (Norway rat)	RT1-CE3	NP_001008841.1
Gallus gallus (chicken)	BF1	XP_004945708.1
Gallus gallus (chicken)	LOC417058	XP_004950006.1
Gallus gallus (chicken)	LOC417057	XP_415345.4
Gallus gallus (chicken)	LOC100859282	XP_003642295.2
Mus musculus (house mouse)	H2-Q10	NP_034521.1
Gallus gallus (chicken)	LOC430600	XP_428156.3
Danio rerio (zebrafish)	mhc1zba	NP_001103588.1
Gallus gallus (chicken)	LOC100859807	XP_003643267.1
Gallus gallus (chicken)	LOC100859881	XP_003643268.1
Gallus gallus (chicken)	LOC100859012	XP_003643257.1
Mus musculus (house mouse)	H2-Q6	NP_997531.1
Rattus norvegicus (Norway rat)	RT1-CE13	NP_001008836.1
Rattus norvegicus (Norway rat)	RT1-CE4	NP_001008842.1
Gallus gallus (chicken)	LOC768350	NP_001171648.1
Gallus gallus (chicken)	LOC417080	XP_003643749.2
Danio rerio (zebrafish)	si:ch211-287j19.6	NP_001039028.1
Danio rerio (zebrafish)	LOC556263	XP_003198042.1
Danio rerio (zebrafish)	mhc1zja	NP_001103188.1
Homo sapiens (human)	HLA-A	NP_002107.3
Pan troglodytes (chimpanzee)	PATR-A	NP_001009058.2
Mus musculus (house mouse)	H2-K1	NP_001001892.2
Gallus gallus (chicken)	LOC100857553	XP_003643732.2
Gallus gallus (chicken)	LOC100858943	XP_003643255.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A nonclassical MHC class I U lineage locus in zebrafish with a null haplotypic variant.
Dirscherl H, Yoder JA
Immunogenetics67(9)501-13(2015 Sep)

The MHC class I genes of zebrafish.
Dirscherl H, McConnell SC, Yoder JA, de Jong JL
Developmental and comparative immunology46(1)11-23(2014 Sep)

Characterization of the Z lineage Major histocompatability complex class I genes in zebrafish.
Dirscherl H, Yoder JA
Immunogenetics66(3)185-98(2014 Mar)

Multiple divergent haplotypes express completely distinct sets of class I MHC genes in zebrafish.
McConnell SC, Restaino AC, de Jong JL
Immunogenetics66(3)199-213(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mhc1zca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mhc1zca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02401	NM_001083545.1 Latest version!	Danio rerio major histocompatibility complex class I ZCA (mhc1zca), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa147250	XM_021474930.1 Latest version!	Danio rerio major histocompatibility complex class I ZCA (mhc1zca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa53703	XM_017354912.1 Latest version!	Danio rerio zgc:136614 (zgc:136614), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mhc1zca ( NM_001083545.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077014.1 Danio rerio major histocompatibility complex class I ZCA (mhc1zca), mRNA.

Price & Availability↑

CloneID	ODa02401
Clone ID Related Accession (Same CDS sequence)	NM_001083545.1
Accession Version	NM_001083545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501862400000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC556341 precursor
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC115196.1. On or before Jan 14, 2017 this sequence version replaced NM_001039986.1, XM_679111.2. ##Evidence-Data-START## Transcript exon combination :: BC115196.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGTTT TGGCCGTTTT GTTTTCGGCT GTGATGCTCC TTTCTGTTGT ATCAGTCCGG 
ACCGAAAAAC ACTCCCTCTA CTACATATAC ACAGCTTTAT CCAGACCTGT CGACCAGTCG 
GGCATCTATC AATTCACTGC AATGGGTTTG CTTGATGACA GACAGATTGA CTATTACAAT 
AACATAGATC AGAAGAAGAT TCCTAAACAG CACTGGATGA AAGAGAAAAT GCAGGAAGAT 
TACTGGGAAA AAGGCACCCA GTCCAGAAAG AGCAAAGAAC AGTGGTTTAA TGTGAACGTC 
AACATTCTGA TGGACCGAAT GAGACACAAT AATTCAGATC TTCATGTTCT TCAGTGGAGA 
CATGGTTGTG AAATTGAGAT TCAGGGGAAT GAACACCGCT TTTCTAAAGG CATTGATGAG 
TACAGCTATG ATGGAAGAAA CTTCCTTTCT TTTGATGATG CGGAGTCTCA GTGGGTTGCC 
CCAGTGGACG CAGCTCTTCC AACCAAGAGG AAATGGGATA ATGTGCCCAT CCTAAATCAG 
TACACCAAGG GCTACCTGGA AAAAGAGTGT GTGGACTGGC TCAACAAATT CAGAGAATAC 
GGAGATCAGG AGCTTAGAGA CGGCTCTCCC CCAGATGTTT ATGTGTTTGC AAGAAGGATT 
ATCAGTGGCA AAATCAAGCT AAAACTTACC TGTATGGCCA CTGGATTCTA CCCCAAAGAT 
ATGATTTTGA CTATAAGGAA ATACCGCACG ACACTGCCTG ATAATGATCT AGATTCCTCA 
GGAGTTAGAC CAAACCAGGA TGGGACCTTT CAGCTGAGAA AGAGCACTAA CATCTATGAG 
GATGAAAAAG CAGAATATGA CTGTTATGTG AACCACAGAA CCCTCAAGGA ACCAATTATC 
GTCAGATGGA ATGGTGAATA CCTTTCAGAG CCTCCCATAG CCATCATTGC AGCAATAATT 
GGAGTTTTGA TTCTGCTGGT AGCCATTGGT GTAACAGTTT GGATCTTAAA AAAGAAGAAC 
ATAATTGGAA ATAAGGATGA GAAACGCTCT ATGCCAAATG GTTCGGCCAA CTATGGTAGA 
GGGAGTAGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077014.1
CDS	41..1135
Translation	MAVLAVLFSAVMLLSVVSVRTEKHSLYYIYTALSRPVDQSGIYQFTAMGLLDDRQIDYYNNIDQKKIPKQHWMKEKMQEDYWEKGTQSRKSKEQWFNVNVNILMDRMRHNNSDLHVLQWRHGCEIEIQGNEHRFSKGIDEYSYDGRNFLSFDDAESQWVAPVDAALPTKRKWDNVPILNQYTKGYLEKECVDWLNKFREYGDQELRDGSPPDVYVFARRIISGKIKLKLTCMATGFYPKDMILTIRKYRTTLPDNDLDSSGVRPNQDGTFQLRKSTNIYEDEKAEYDCYVNHRTLKEPIIVRWNGEYLSEPPIAIIAAIIGVLILLVAIGVTVWILKKKNIIGNKDEKRSMPNGSANYGRGSSA

Target ORF information:

RefSeq Version	NM_001083545.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio major histocompatibility complex class I ZCA (mhc1zca), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083545.1

ORF Insert Sequence:

ATGGCTGTTT TGGCCGTTTT GTTTTCGGCT GTGATGCTCC TTTCTGTTGT ATCAGTCCGG 
ACCGAAAAAC ACTCCCTCTA CTACATATAC ACAGCTTTAT CCAGACCTGT CGACCAGTCG 
GGCATCTATC AATTCACTGC AATGGGTTTG CTTGATGACA GACAGATTGA CTATTACAAT 
AACATAGATC AGAAGAAGAT TCCTAAACAG CACTGGATGA AAGAGAAAAT GCAGGAAGAT 
TACTGGGAAA AAGGCACCCA GTCCAGAAAG AGCAAAGAAC AGTGGTTTAA TGTGAACGTC 
AACATTCTGA TGGACCGAAT GAGACACAAT AATTCAGATC TTCATGTTCT TCAGTGGAGA 
CATGGTTGTG AAATTGAGAT TCAGGGGAAT GAACACCGCT TTTCTAAAGG CATTGATGAG 
TACAGCTATG ATGGAAGAAA CTTCCTTTCT TTTGATGATG CGGAGTCTCA GTGGGTTGCC 
CCAGTGGACG CAGCTCTTCC AACCAAGAGG AAATGGGATA ATGTGCCCAT CCTAAATCAG 
TACACCAAGG GCTACCTGGA AAAAGAGTGT GTGGACTGGC TCAACAAATT CAGAGAATAC 
GGAGATCAGG AGCTTAGAGA CGGCTCTCCC CCAGATGTTT ATGTGTTTGC AAGAAGGATT 
ATCAGTGGCA AAATCAAGCT AAAACTTACC TGTATGGCCA CTGGATTCTA CCCCAAAGAT 
ATGATTTTGA CTATAAGGAA ATACCGCACG ACACTGCCTG ATAATGATCT AGATTCCTCA 
GGAGTTAGAC CAAACCAGGA TGGGACCTTT CAGCTGAGAA AGAGCACTAA CATCTATGAG 
GATGAAAAAG CAGAATATGA CTGTTATGTG AACCACAGAA CCCTCAAGGA ACCAATTATC 
GTCAGATGGA ATGGTGAATA CCTTTCAGAG CCTCCCATAG CCATCATTGC AGCAATAATT 
GGAGTTTTGA TTCTGCTGGT AGCCATTGGT GTAACAGTTT GGATCTTAAA AAAGAAGAAC 
ATAATTGGAA ATAAGGATGA GAAACGCTCT ATGCCAAATG GTTCGGCCAA CTATGGTAGA 
GGGAGTAGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mhc1zca ( XM_021474930.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330605.1 Danio rerio major histocompatibility complex class I ZCA (mhc1zca), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa147250
Clone ID Related Accession (Same CDS sequence)	XM_021474930.1
Accession Version	XM_021474930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC556341 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTT TGGCCGTTTT GTTTTCGGCT GTGATGCTCC TTTCTGTTGT ATCAGTCCGG 
ACCGAAAAAC ACTCCCTCTA CTACATATAC ACAGCTTTAT CCAGACCTGT CGACCAGTCG 
GGCATCTATC AATTCACTGC AATGGGTTTG CTTGATGACA GACAGATTGA CTATTACAAT 
AACATAGATC AGAAGAAGAT TCCTAAACAG CACTGGATGA AAGAGAAAAT GCAGGAAGAT 
TACTGGGAAA AAGGCACCCA GTCCAGAAAG AGCAAAGAAC AGTGGTTTAA TGTGAACGTC 
AACATTCTGA TGGACCGAAT GAGACACAAT AATTCAGATC TTCATGTTCT TCAGTGGAGA 
CATGGTTGTG AAATTGAGAT TCAGGGGAAT GAACACCGCT TTTCTAAAGG CATTGATGAG 
TACAGCTATG ATGGAAGAAA CTTCCTTTCT TTTGATGATG CGGAGTCTCA GTGGGTTGCC 
CCAGTGGACG CAGCTCTTCC AACCAAGAGG AAATGGGATA ATGTGCCCAT CCTAAATCAG 
TACACCAAGG GCTACCTGGA AAAAGAGTGT GTGGACTGGC TCAACAAATT CAGAGAATAC 
GGAGATCAGG AGCTTAGAGA CGGCTATGGT GAATACCTTT CAGAGCCTCC CATAGCCATC 
ATTGCAGCAA TAATTGGAGT TTTGATTCTG CTGGTAGCCA TTGGTGTAAC AGTTTGGATC 
TTAAAAAAGA AGAACATAAT TGGAAATAAG GATGAGAAAC GCTCTATGCC AAATGGTTCG 
GCCAACTATG GTAGAGGGAG TAGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330605.1
CDS	89..898
Translation	MAVLAVLFSAVMLLSVVSVRTEKHSLYYIYTALSRPVDQSGIYQFTAMGLLDDRQIDYYNNIDQKKIPKQHWMKEKMQEDYWEKGTQSRKSKEQWFNVNVNILMDRMRHNNSDLHVLQWRHGCEIEIQGNEHRFSKGIDEYSYDGRNFLSFDDAESQWVAPVDAALPTKRKWDNVPILNQYTKGYLEKECVDWLNKFREYGDQELRDGYGEYLSEPPIAIIAAIIGVLILLVAIGVTVWILKKKNIIGNKDEKRSMPNGSANYGRGSSA

Target ORF information:

RefSeq Version	XM_021474930.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio major histocompatibility complex class I ZCA (mhc1zca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474930.1

ORF Insert Sequence:

ATGGCTGTTT TGGCCGTTTT GTTTTCGGCT GTGATGCTCC TTTCTGTTGT ATCAGTCCGG 
ACCGAAAAAC ACTCCCTCTA CTACATATAC ACAGCTTTAT CCAGACCTGT CGACCAGTCG 
GGCATCTATC AATTCACTGC AATGGGTTTG CTTGATGACA GACAGATTGA CTATTACAAT 
AACATAGATC AGAAGAAGAT TCCTAAACAG CACTGGATGA AAGAGAAAAT GCAGGAAGAT 
TACTGGGAAA AAGGCACCCA GTCCAGAAAG AGCAAAGAAC AGTGGTTTAA TGTGAACGTC 
AACATTCTGA TGGACCGAAT GAGACACAAT AATTCAGATC TTCATGTTCT TCAGTGGAGA 
CATGGTTGTG AAATTGAGAT TCAGGGGAAT GAACACCGCT TTTCTAAAGG CATTGATGAG 
TACAGCTATG ATGGAAGAAA CTTCCTTTCT TTTGATGATG CGGAGTCTCA GTGGGTTGCC 
CCAGTGGACG CAGCTCTTCC AACCAAGAGG AAATGGGATA ATGTGCCCAT CCTAAATCAG 
TACACCAAGG GCTACCTGGA AAAAGAGTGT GTGGACTGGC TCAACAAATT CAGAGAATAC 
GGAGATCAGG AGCTTAGAGA CGGCTATGGT GAATACCTTT CAGAGCCTCC CATAGCCATC 
ATTGCAGCAA TAATTGGAGT TTTGATTCTG CTGGTAGCCA TTGGTGTAAC AGTTTGGATC 
TTAAAAAAGA AGAACATAAT TGGAAATAAG GATGAGAAAC GCTCTATGCC AAATGGTTCG 
GCCAACTATG GTAGAGGGAG TAGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mhc1zca ( XM_017354912.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210401.1 Danio rerio zgc:136614 (zgc:136614), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa53703
Clone ID Related Accession (Same CDS sequence)	XM_017354912.1
Accession Version	XM_017354912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC556341 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTT TGGCCGTTTT GTTTTCGGCT GTGATGCTCC TTTCTGTTGT ATCAGTCCGG 
ACCGAAAAAC ACTCCCTCTA CTACATATAC ACAGCTTTAT CCAGACCTGT CGACCAGTCG 
GGCATCTATC AATTCACTGC AATGGGTTTG CTTGATGACA GACAGATTGA CTATTACAAT 
AACATAGATC AGAAGAAGAT TCCTAAACAG CACTGGATGA AAGAGAAAAT GCAGGAAGAT 
TACTGGGAAA AAGGCACCCA GTCCAGAAAG AGCAAAGAAC AGTGGTTTAA TGTGAACGTC 
AACATTCTGA TGGACCGAAT GAGACACAAT AATTCAGATC TTCATGTTCT TCAGTGGAGA 
CATGGTTGTG AAATTGAGAT TCAGGGGAAT GAACACCGCT TTTCTAAAGG CATTGATGAG 
TACAGCTATG ATGGAAGAAA CTTCCTTTCT TTTGATGATG CGGAGTCTCA GTGGGTTGCC 
CCAGTGGACG CAGCTCTTCC AACCAAGAGG AAATGGGATA ATGTGCCCAT CCTAAATCAG 
TACACCAAGG GCTACCTGGA AAAAGAGTGT GTGGACTGGC TCAACAAATT CAGAGAATAC 
GGAGATCAGG AGCTTAGAGA CGGCTCTCCC CCAGATGTTT ATGTGTTTGC AAGAAGGATT 
ATCAGTGGCA AAATCAAGCT AAAACTTACC TGTATGGCCA CTGGATTCTA CCCCAAAGAT 
ATGATTTTGA CTATAAGGAA ATACCGCACG ACACTGCCTG ATAATGATCT AGATTCCTCA 
GGAGTTAGAC CAAACCAGGA TGGGACCTTT CAGCTGAGAA AGAGCACTAA CATCTATGAG 
GATGAAAAAG CAGAATATGA CTGTTATGTG AACCACAGAA CCCTCAAGGA ACCAATTATC 
GTCAGATGGA ATGGTGAATA CCTTTCAGAG CCTCCCATAG CCATCATTGC AGCAATAATT 
GGAGTTTTGA TTCTGCTGGT AGCCATTGGT GTAACAGTTT GGATCTTAAA AAAGAAGAAC 
ATAATTGGAA ATAAGGATGA GAAACGCTTT GTCGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210401.1
CDS	65..1123
Translation	MAVLAVLFSAVMLLSVVSVRTEKHSLYYIYTALSRPVDQSGIYQFTAMGLLDDRQIDYYNNIDQKKIPKQHWMKEKMQEDYWEKGTQSRKSKEQWFNVNVNILMDRMRHNNSDLHVLQWRHGCEIEIQGNEHRFSKGIDEYSYDGRNFLSFDDAESQWVAPVDAALPTKRKWDNVPILNQYTKGYLEKECVDWLNKFREYGDQELRDGSPPDVYVFARRIISGKIKLKLTCMATGFYPKDMILTIRKYRTTLPDNDLDSSGVRPNQDGTFQLRKSTNIYEDEKAEYDCYVNHRTLKEPIIVRWNGEYLSEPPIAIIAAIIGVLILLVAIGVTVWILKKKNIIGNKDEKRFVV

Target ORF information:

RefSeq Version	XM_017354912.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:136614 (zgc:136614), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354912.1

ORF Insert Sequence:

ATGGCTGTTT TGGCCGTTTT GTTTTCGGCT GTGATGCTCC TTTCTGTTGT ATCAGTCCGG 
ACCGAAAAAC ACTCCCTCTA CTACATATAC ACAGCTTTAT CCAGACCTGT CGACCAGTCG 
GGCATCTATC AATTCACTGC AATGGGTTTG CTTGATGACA GACAGATTGA CTATTACAAT 
AACATAGATC AGAAGAAGAT TCCTAAACAG CACTGGATGA AAGAGAAAAT GCAGGAAGAT 
TACTGGGAAA AAGGCACCCA GTCCAGAAAG AGCAAAGAAC AGTGGTTTAA TGTGAACGTC 
AACATTCTGA TGGACCGAAT GAGACACAAT AATTCAGATC TTCATGTTCT TCAGTGGAGA 
CATGGTTGTG AAATTGAGAT TCAGGGGAAT GAACACCGCT TTTCTAAAGG CATTGATGAG 
TACAGCTATG ATGGAAGAAA CTTCCTTTCT TTTGATGATG CGGAGTCTCA GTGGGTTGCC 
CCAGTGGACG CAGCTCTTCC AACCAAGAGG AAATGGGATA ATGTGCCCAT CCTAAATCAG 
TACACCAAGG GCTACCTGGA AAAAGAGTGT GTGGACTGGC TCAACAAATT CAGAGAATAC 
GGAGATCAGG AGCTTAGAGA CGGCTCTCCC CCAGATGTTT ATGTGTTTGC AAGAAGGATT 
ATCAGTGGCA AAATCAAGCT AAAACTTACC TGTATGGCCA CTGGATTCTA CCCCAAAGAT 
ATGATTTTGA CTATAAGGAA ATACCGCACG ACACTGCCTG ATAATGATCT AGATTCCTCA 
GGAGTTAGAC CAAACCAGGA TGGGACCTTT CAGCTGAGAA AGAGCACTAA CATCTATGAG 
GATGAAAAAG CAGAATATGA CTGTTATGTG AACCACAGAA CCCTCAAGGA ACCAATTATC 
GTCAGATGGA ATGGTGAATA CCTTTCAGAG CCTCCCATAG CCATCATTGC AGCAATAATT 
GGAGTTTTGA TTCTGCTGGT AGCCATTGGT GTAACAGTTT GGATCTTAAA AAAGAAGAAC 
ATAATTGGAA ATAAGGATGA GAAACGCTTT GTCGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Multiple divergent haplotypes express completely distinct sets of class I MHC genes in zebrafish.
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mhc1zca cDNA ORF clone, Danio rerio(zebrafish)

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