mhc1zka cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mhc1zka
Entrez Gene ID	100536229
Full Name	major histocompatibility complex class I ZKA
General protein information	Preferred Names major histocompatibility complex class I ZKA Names uncharacterized protein LOC100536229 MHC class I antigen ZKA
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017354485.2	XP_017209974.1	uncharacterized protein LOC100536229 isoform X1
NM_001302245.1	NP_001289174.1	uncharacterized protein LOC100536229 precursor
XM_017354485.1	XP_017209974.1	uncharacterized protein LOC100536229 isoform X1
XM_009303558.2	XP_009301833.1	uncharacterized protein LOC100536229 isoform X2
XM_021470907.1	XP_021326582.1	uncharacterized protein LOC100536229 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gm8909	NP_001074501.2
Mus musculus (house mouse)	H2-Bl	NP_032225.2
Mus musculus (house mouse)	H2-Q2	NP_034522.2
Rattus norvegicus (Norway rat)	RT1-A1	NP_001008827.1
Rattus norvegicus (Norway rat)	RT1-CE2	NP_001008840.1
Rattus norvegicus (Norway rat)	RT1-CE5	NP_001008843.1
Rattus norvegicus (Norway rat)	RT1-A2	NP_001008829.1
Gallus gallus (chicken)	MICA	XP_415354.4
Gallus gallus (chicken)	MR1	XP_004945738.1
Gallus gallus (chicken)	LOC417083	XP_003642294.2
Danio rerio (zebrafish)	zgc:136614	NP_001077014.1
Gallus gallus (chicken)	LOC100858733	XP_003643847.2
Gallus gallus (chicken)	LOC100858581	XP_003643753.2
Rattus norvegicus (Norway rat)	LOC100364500	XP_006255953.1
Danio rerio (zebrafish)	LOC100536229	XP_003201722.1
Macaca mulatta (Rhesus monkey)	MAMU-A3	NP_001165309.1
Mus musculus (house mouse)	H2-D1	NP_034510.3
Mus musculus (house mouse)	Gm10499	XP_006525296.1
Mus musculus (house mouse)	H2-Q4	NP_001137161.1
Mus musculus (house mouse)	H2-Q7	NP_034524.3
Rattus norvegicus (Norway rat)	RT1-CE7	NP_001008845.1
Rattus norvegicus (Norway rat)	RT1-CE1	NP_001008832.1
Rattus norvegicus (Norway rat)	RT1-CE10	NP_001008833.1
Gallus gallus (chicken)	BF2	NP_001026509.1
Gallus gallus (chicken)	LOC769716	XP_001233005.3
Gallus gallus (chicken)	YF6	XP_003643784.1
Danio rerio (zebrafish)	LOC563036	XP_001919291.4
Gallus gallus (chicken)	LOC101752075	XP_004950216.1
Gallus gallus (chicken)	LOC100859374	XP_003643261.1
Gallus gallus (chicken)	LOC100859408	XP_003643262.1
Gallus gallus (chicken)	LOC100859100	XP_003642292.2
Mus musculus (house mouse)	H2-Q1	NP_034520.2
Rattus norvegicus (Norway rat)	RT1-CE16	NP_001008839.1
Rattus norvegicus (Norway rat)	RT1-CE3	NP_001008841.1
Gallus gallus (chicken)	BF1	XP_004945708.1
Gallus gallus (chicken)	LOC417058	XP_004950006.1
Gallus gallus (chicken)	LOC417057	XP_415345.4
Gallus gallus (chicken)	LOC100859282	XP_003642295.2
Mus musculus (house mouse)	H2-Q10	NP_034521.1
Gallus gallus (chicken)	LOC430600	XP_428156.3
Danio rerio (zebrafish)	mhc1zba	NP_001103588.1
Gallus gallus (chicken)	LOC100859807	XP_003643267.1
Gallus gallus (chicken)	LOC100859881	XP_003643268.1
Gallus gallus (chicken)	LOC100859012	XP_003643257.1
Mus musculus (house mouse)	H2-Q6	NP_997531.1
Rattus norvegicus (Norway rat)	RT1-CE13	NP_001008836.1
Rattus norvegicus (Norway rat)	RT1-CE4	NP_001008842.1
Gallus gallus (chicken)	LOC768350	NP_001171648.1
Gallus gallus (chicken)	LOC417080	XP_003643749.2
Danio rerio (zebrafish)	si:ch211-287j19.6	NP_001039028.1
Danio rerio (zebrafish)	LOC556263	XP_003198042.1
Danio rerio (zebrafish)	mhc1zja	NP_001103188.1
Homo sapiens (human)	HLA-A	NP_002107.3
Pan troglodytes (chimpanzee)	PATR-A	NP_001009058.2
Mus musculus (house mouse)	H2-K1	NP_001001892.2
Gallus gallus (chicken)	LOC100857553	XP_003643732.2
Gallus gallus (chicken)	LOC100858943	XP_003643255.2

Gene Ontology
Bibliography

Related articles in PubMed

RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways.
Wu XM, Chen WQ, Hu YW, Cao L, Nie P, Chang MX
Frontiers in immunology9726(2018)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The identification of additional zebrafish DICP genes reveals haplotype variation and linkage to MHC class I genes.
Rodriguez-Nunez I, Wcisel DJ, Litman RT, Litman GW, Yoder JA
Immunogenetics68(4)295-312(2016 Apr)

A nonclassical MHC class I U lineage locus in zebrafish with a null haplotypic variant.
Dirscherl H, Yoder JA
Immunogenetics67(9)501-13(2015 Sep)

The MHC class I genes of zebrafish.
Dirscherl H, McConnell SC, Yoder JA, de Jong JL
Developmental and comparative immunology46(1)11-23(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mhc1zka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mhc1zka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa50314	NM_001302245.1 Latest version!	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa50313	XM_009303558.2 Latest version!	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa50314	XM_021470907.1 Latest version!	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa53485	XM_017354485.2 Latest version!	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa53485	XM_017354485.1	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mhc1zka ( NM_001302245.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001289174.1 Danio rerio major histocompatibility complex class I ZKA (mhc1zka), mRNA.

Price & Availability↑

CloneID	ODa50314
Clone ID Related Accession (Same CDS sequence)	NM_001302245.1 , XM_021470907.1
Accession Version	NM_001302245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1535731200000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100536229 precursor
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from KC607870.1. ##Evidence-Data-START## Transcript exon combination :: KC607870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGTCAG GAGGAGTCGC GAGCGGGATC TGTGCTCTGC TCTGTGTGTT TCTGCTGTGT 
GGGGACTTGC CGTCAGCTCA GGGCGAGAAG CACTCTCTGT ATTACATCTA CACGGGTTTG 
TCCAAACCTC TGGATCTGCC GGGCATCTAT GAGTTCTCTG CTATGGGTCT GTTAGATGAC 
AGACAGATTG ACTCTTATAA CAGCGAAGAG CAGAGAAAGA TTCCCAAACA GCAGTGGATG 
AAGGAGAAAA TGCAGGAGGA TTACTGGGAG AAAGGCACTC AGTCGAGAAA GAGCAAAGAG 
CAGTGGTTTA ATGTGAACGT CCACATACTG ATGGACCGCA TGAGACACAA TAAATCAGAT 
CTTCACGTTC TTCAGTGGAG ACACGGCTGT GAAGTGGAAA TACAAGGAAG TGAAGTGAAG 
TTTTCCAAAG GCGTTAGCGA GTATGGCTAT GATGGAGAAA ACCTCCTGTC TTTTGATGAG 
ACAGAGTCTC AGTGGGTCGC CCCAGTGAAA GAAGCGCTGC CAACCAAGAG AAAATGGGAC 
AATGTGCCCA TCCTGAACCA ATACACCAAA GGCTACCTGG AGAAAGAGTG TGTGGACTGG 
CTCAACAAAT TCAGAGAGTA TGCAGATGCA GAGCTCAGAA ATGGCTCTCC TCCAGATGTT 
TATAAATTCA CAAAAAAGTC CACTAAAGAT GAAACTAAGC TGAAACTCAC CTGTCTGGCC 
ACTGGCTTCT ACCCCAGCGA CGTGATGTTG AACATTAGGA GAAATCGCGT ATATCTGCCT 
GAACAAGAGA CCATATCTAC AGGAGTTCGA CCAAACCATG ATCAAACCTT CCAGCTGAGC 
AAGAGTGTGG AGATCAAGGA GGACCAGATA GATGAATATG ATTGTTCTGT GTCCCACAGA 
ACCTTCAAAC AACCAATTAT CATAAAATGG GATGGAACAG ATTTAGATGG AAAATACACC 
GGCTACCCAC CAGAGACTGT CCCAGTTGTG ATCGGAAGTG TGCTTATTTT ATTAGCCATT 
TTAGCTGTAG TGGTTTTTCT GGTGATGAAT TATGATGGTG AGTGTTGCAA AGGGCTCTTT 
ATTCACTTCA CCCTTCTTAA GGACTGTGGA CTGTTGCTGC CCCCTTGTGG AAAGACTGAG 
TATGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289174.1
CDS	1..1149
Translation	MMSGGVASGICALLCVFLLCGDLPSAQGEKHSLYYIYTGLSKPLDLPGIYEFSAMGLLDDRQIDSYNSEEQRKIPKQQWMKEKMQEDYWEKGTQSRKSKEQWFNVNVHILMDRMRHNKSDLHVLQWRHGCEVEIQGSEVKFSKGVSEYGYDGENLLSFDETESQWVAPVKEALPTKRKWDNVPILNQYTKGYLEKECVDWLNKFREYADAELRNGSPPDVYKFTKKSTKDETKLKLTCLATGFYPSDVMLNIRRNRVYLPEQETISTGVRPNHDQTFQLSKSVEIKEDQIDEYDCSVSHRTFKQPIIIKWDGTDLDGKYTGYPPETVPVVIGSVLILLAILAVVVFLVMNYDGECCKGLFIHFTLLKDCGLLLPPCGKTEYV

Target ORF information:

RefSeq Version	NM_001302245.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302245.1

ORF Insert Sequence:

ATGATGTCAG GAGGAGTCGC GAGCGGGATC TGTGCTCTGC TCTGTGTGTT TCTGCTGTGT 
GGGGACTTGC CGTCAGCTCA GGGCGAGAAG CACTCTCTGT ATTACATCTA CACGGGTTTG 
TCCAAACCTC TGGATCTGCC GGGCATCTAT GAGTTCTCTG CTATGGGTCT GTTAGATGAC 
AGACAGATTG ACTCTTATAA CAGCGAAGAG CAGAGAAAGA TTCCCAAACA GCAGTGGATG 
AAGGAGAAAA TGCAGGAGGA TTACTGGGAG AAAGGCACTC AGTCGAGAAA GAGCAAAGAG 
CAGTGGTTTA ATGTGAACGT CCACATACTG ATGGACCGCA TGAGACACAA TAAATCAGAT 
CTTCACGTTC TTCAGTGGAG ACACGGCTGT GAAGTGGAAA TACAAGGAAG TGAAGTGAAG 
TTTTCCAAAG GCGTTAGCGA GTATGGCTAT GATGGAGAAA ACCTCCTGTC TTTTGATGAG 
ACAGAGTCTC AGTGGGTCGC CCCAGTGAAA GAAGCGCTGC CAACCAAGAG AAAATGGGAC 
AATGTGCCCA TCCTGAACCA ATACACCAAA GGCTACCTGG AGAAAGAGTG TGTGGACTGG 
CTCAACAAAT TCAGAGAGTA TGCAGATGCA GAGCTCAGAA ATGGCTCTCC TCCAGATGTT 
TATAAATTCA CAAAAAAGTC CACTAAAGAT GAAACTAAGC TGAAACTCAC CTGTCTGGCC 
ACTGGCTTCT ACCCCAGCGA CGTGATGTTG AACATTAGGA GAAATCGCGT ATATCTGCCT 
GAACAAGAGA CCATATCTAC AGGAGTTCGA CCAAACCATG ATCAAACCTT CCAGCTGAGC 
AAGAGTGTGG AGATCAAGGA GGACCAGATA GATGAATATG ATTGTTCTGT GTCCCACAGA 
ACCTTCAAAC AACCAATTAT CATAAAATGG GATGGAACAG ATTTAGATGG AAAATACACC 
GGCTACCCAC CAGAGACTGT CCCAGTTGTG ATCGGAAGTG TGCTTATTTT ATTAGCCATT 
TTAGCTGTAG TGGTTTTTCT GGTGATGAAT TATGATGGTG AGTGTTGCAA AGGGCTCTTT 
ATTCACTTCA CCCTTCTTAA GGACTGTGGA CTGTTGCTGC CCCCTTGTGG AAAGACTGAG 
TATGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mhc1zka ( XM_009303558.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301833.1 Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa50313
Clone ID Related Accession (Same CDS sequence)	XM_009303558.2
Accession Version	XM_009303558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100536229 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336703.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCAG GAGGAGTCGC GAGCGGGATC TGTGCTCTGC TCTGTGTGTT TCTGCTGTGT 
GGGGACTTGC CGTCAGCTCA GGGCGAGAAG CACTCTCTGT ATTACATCTA CACGGGTTTG 
TCCAAACCTC TGGATCTGCC GGGCATCTAT GAGTTCTCTG CTATGGGTCT GTTAGATGAC 
AGACAGATTG ACTCTTATAA CAGCGAAGAG CAGAGAAAGA TTCCCAAACA GCAGTGGATG 
AAGGAGAAAA TGCAGGAGGA TTACTGGGAG AAAGGCACTC AGTCGAGAAA GAGCAAAGAG 
CAGTGGTTTA ATGTGAACGT CCACATACTG ATGGACCGCA TGAGACACAA TAAATCAGAT 
CTTCACGTTC TTCAGTGGAG ACACGGCTGT GAAGTGGAAA TACAAGGAAG TGAAGTGAAG 
TTTTCCAAAG GCGTTAGCGA GTATGGCTAT GATGGAGAAA ACCTCCTGTC TTTTGATGAG 
ACAGAGTCTC AGTGGGTCGC CCCAGTGAAA GAAGCGCTGC CAACCAAGAG AAAATGGGAC 
AATGTGCCCA TCCTGAACCA ATACACCAAA GGCTACCTGG AGAAAGAGTG TGTGGACTGG 
CTCAACAAAT TCAGAGAGTA TGCAGATGCA GAGCTCAGAA ATGGCTCTCC TCCAGATGTT 
TATAAATTCA CAAAAAAGTC CACTAAAGAT GAAACTAAGC TGAAACTCAC CTGTCTGGCC 
ACTGGCTTCT ACCCCAGCGA CGTGATGTTG AACATTAGGA GAAATCGCGT ATATCTGCCT 
GAACAAGAGA CCATATCTAC AGGAGTTCGA CCAAACCATG ATCAAACCTT CCAGCTGAGC 
AAGAGTGTGG AGATCAAGGA GGACCAGATA GATGAATATG ATTGTTCTGT GTCCCACAGA 
ACCTTCAAAC AACCAATTAT CATAAAATGG GATGGAACAG ATTTAGATGG AAAATACACC 
GGCTACCCAC CAGAGACTGT CCCAGTTGTG ATCGGAAGTG TGCTTATTTT ATTAGCCATT 
TTAGCTGTAG TGGTTTTTCT GGTGATGAAT TATGATGCTG TCTGCCTCCT CAGAGAGTTA 
CAGAAAACAG AATCACAGAA GACGCTACAG AATCAGTCTT CAACGACAGA ACAGGACAGC 
AGCAGCACAG ACACCACTGA GTGTACCTCA ATATTGCTGG GATTTATTGC TGACCATCTG 
AATAAAGAAT TAACGGAAGG AGAGAATTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301833.1
CDS	140..1372
Translation	MMSGGVASGICALLCVFLLCGDLPSAQGEKHSLYYIYTGLSKPLDLPGIYEFSAMGLLDDRQIDSYNSEEQRKIPKQQWMKEKMQEDYWEKGTQSRKSKEQWFNVNVHILMDRMRHNKSDLHVLQWRHGCEVEIQGSEVKFSKGVSEYGYDGENLLSFDETESQWVAPVKEALPTKRKWDNVPILNQYTKGYLEKECVDWLNKFREYADAELRNGSPPDVYKFTKKSTKDETKLKLTCLATGFYPSDVMLNIRRNRVYLPEQETISTGVRPNHDQTFQLSKSVEIKEDQIDEYDCSVSHRTFKQPIIIKWDGTDLDGKYTGYPPETVPVVIGSVLILLAILAVVVFLVMNYDAVCLLRELQKTESQKTLQNQSSTTEQDSSSTDTTECTSILLGFIADHLNKELTEGENW

Target ORF information:

RefSeq Version	XM_009303558.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303558.2

ORF Insert Sequence:

ATGATGTCAG GAGGAGTCGC GAGCGGGATC TGTGCTCTGC TCTGTGTGTT TCTGCTGTGT 
GGGGACTTGC CGTCAGCTCA GGGCGAGAAG CACTCTCTGT ATTACATCTA CACGGGTTTG 
TCCAAACCTC TGGATCTGCC GGGCATCTAT GAGTTCTCTG CTATGGGTCT GTTAGATGAC 
AGACAGATTG ACTCTTATAA CAGCGAAGAG CAGAGAAAGA TTCCCAAACA GCAGTGGATG 
AAGGAGAAAA TGCAGGAGGA TTACTGGGAG AAAGGCACTC AGTCGAGAAA GAGCAAAGAG 
CAGTGGTTTA ATGTGAACGT CCACATACTG ATGGACCGCA TGAGACACAA TAAATCAGAT 
CTTCACGTTC TTCAGTGGAG ACACGGCTGT GAAGTGGAAA TACAAGGAAG TGAAGTGAAG 
TTTTCCAAAG GCGTTAGCGA GTATGGCTAT GATGGAGAAA ACCTCCTGTC TTTTGATGAG 
ACAGAGTCTC AGTGGGTCGC CCCAGTGAAA GAAGCGCTGC CAACCAAGAG AAAATGGGAC 
AATGTGCCCA TCCTGAACCA ATACACCAAA GGCTACCTGG AGAAAGAGTG TGTGGACTGG 
CTCAACAAAT TCAGAGAGTA TGCAGATGCA GAGCTCAGAA ATGGCTCTCC TCCAGATGTT 
TATAAATTCA CAAAAAAGTC CACTAAAGAT GAAACTAAGC TGAAACTCAC CTGTCTGGCC 
ACTGGCTTCT ACCCCAGCGA CGTGATGTTG AACATTAGGA GAAATCGCGT ATATCTGCCT 
GAACAAGAGA CCATATCTAC AGGAGTTCGA CCAAACCATG ATCAAACCTT CCAGCTGAGC 
AAGAGTGTGG AGATCAAGGA GGACCAGATA GATGAATATG ATTGTTCTGT GTCCCACAGA 
ACCTTCAAAC AACCAATTAT CATAAAATGG GATGGAACAG ATTTAGATGG AAAATACACC 
GGCTACCCAC CAGAGACTGT CCCAGTTGTG ATCGGAAGTG TGCTTATTTT ATTAGCCATT 
TTAGCTGTAG TGGTTTTTCT GGTGATGAAT TATGATGCTG TCTGCCTCCT CAGAGAGTTA 
CAGAAAACAG AATCACAGAA GACGCTACAG AATCAGTCTT CAACGACAGA ACAGGACAGC 
AGCAGCACAG ACACCACTGA GTGTACCTCA ATATTGCTGG GATTTATTGC TGACCATCTG 
AATAAAGAAT TAACGGAAGG AGAGAATTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mhc1zka ( XM_021470907.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326582.1 Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa50314
Clone ID Related Accession (Same CDS sequence)	NM_001302245.1 , XM_021470907.1
Accession Version	XM_021470907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100536229 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336703.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCAG GAGGAGTCGC GAGCGGGATC TGTGCTCTGC TCTGTGTGTT TCTGCTGTGT 
GGGGACTTGC CGTCAGCTCA GGGCGAGAAG CACTCTCTGT ATTACATCTA CACGGGTTTG 
TCCAAACCTC TGGATCTGCC GGGCATCTAT GAGTTCTCTG CTATGGGTCT GTTAGATGAC 
AGACAGATTG ACTCTTATAA CAGCGAAGAG CAGAGAAAGA TTCCCAAACA GCAGTGGATG 
AAGGAGAAAA TGCAGGAGGA TTACTGGGAG AAAGGCACTC AGTCGAGAAA GAGCAAAGAG 
CAGTGGTTTA ATGTGAACGT CCACATACTG ATGGACCGCA TGAGACACAA TAAATCAGAT 
CTTCACGTTC TTCAGTGGAG ACACGGCTGT GAAGTGGAAA TACAAGGAAG TGAAGTGAAG 
TTTTCCAAAG GCGTTAGCGA GTATGGCTAT GATGGAGAAA ACCTCCTGTC TTTTGATGAG 
ACAGAGTCTC AGTGGGTCGC CCCAGTGAAA GAAGCGCTGC CAACCAAGAG AAAATGGGAC 
AATGTGCCCA TCCTGAACCA ATACACCAAA GGCTACCTGG AGAAAGAGTG TGTGGACTGG 
CTCAACAAAT TCAGAGAGTA TGCAGATGCA GAGCTCAGAA ATGGCTCTCC TCCAGATGTT 
TATAAATTCA CAAAAAAGTC CACTAAAGAT GAAACTAAGC TGAAACTCAC CTGTCTGGCC 
ACTGGCTTCT ACCCCAGCGA CGTGATGTTG AACATTAGGA GAAATCGCGT ATATCTGCCT 
GAACAAGAGA CCATATCTAC AGGAGTTCGA CCAAACCATG ATCAAACCTT CCAGCTGAGC 
AAGAGTGTGG AGATCAAGGA GGACCAGATA GATGAATATG ATTGTTCTGT GTCCCACAGA 
ACCTTCAAAC AACCAATTAT CATAAAATGG GATGGAACAG ATTTAGATGG AAAATACACC 
GGCTACCCAC CAGAGACTGT CCCAGTTGTG ATCGGAAGTG TGCTTATTTT ATTAGCCATT 
TTAGCTGTAG TGGTTTTTCT GGTGATGAAT TATGATGGTG AGTGTTGCAA AGGGCTCTTT 
ATTCACTTCA CCCTTCTTAA GGACTGTGGA CTGTTGCTGC CCCCTTGTGG AAAGACTGAG 
TATGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326582.1
CDS	143..1291
Translation	MMSGGVASGICALLCVFLLCGDLPSAQGEKHSLYYIYTGLSKPLDLPGIYEFSAMGLLDDRQIDSYNSEEQRKIPKQQWMKEKMQEDYWEKGTQSRKSKEQWFNVNVHILMDRMRHNKSDLHVLQWRHGCEVEIQGSEVKFSKGVSEYGYDGENLLSFDETESQWVAPVKEALPTKRKWDNVPILNQYTKGYLEKECVDWLNKFREYADAELRNGSPPDVYKFTKKSTKDETKLKLTCLATGFYPSDVMLNIRRNRVYLPEQETISTGVRPNHDQTFQLSKSVEIKEDQIDEYDCSVSHRTFKQPIIIKWDGTDLDGKYTGYPPETVPVVIGSVLILLAILAVVVFLVMNYDGECCKGLFIHFTLLKDCGLLLPPCGKTEYV

Target ORF information:

RefSeq Version	XM_021470907.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470907.1

ORF Insert Sequence:

ATGATGTCAG GAGGAGTCGC GAGCGGGATC TGTGCTCTGC TCTGTGTGTT TCTGCTGTGT 
GGGGACTTGC CGTCAGCTCA GGGCGAGAAG CACTCTCTGT ATTACATCTA CACGGGTTTG 
TCCAAACCTC TGGATCTGCC GGGCATCTAT GAGTTCTCTG CTATGGGTCT GTTAGATGAC 
AGACAGATTG ACTCTTATAA CAGCGAAGAG CAGAGAAAGA TTCCCAAACA GCAGTGGATG 
AAGGAGAAAA TGCAGGAGGA TTACTGGGAG AAAGGCACTC AGTCGAGAAA GAGCAAAGAG 
CAGTGGTTTA ATGTGAACGT CCACATACTG ATGGACCGCA TGAGACACAA TAAATCAGAT 
CTTCACGTTC TTCAGTGGAG ACACGGCTGT GAAGTGGAAA TACAAGGAAG TGAAGTGAAG 
TTTTCCAAAG GCGTTAGCGA GTATGGCTAT GATGGAGAAA ACCTCCTGTC TTTTGATGAG 
ACAGAGTCTC AGTGGGTCGC CCCAGTGAAA GAAGCGCTGC CAACCAAGAG AAAATGGGAC 
AATGTGCCCA TCCTGAACCA ATACACCAAA GGCTACCTGG AGAAAGAGTG TGTGGACTGG 
CTCAACAAAT TCAGAGAGTA TGCAGATGCA GAGCTCAGAA ATGGCTCTCC TCCAGATGTT 
TATAAATTCA CAAAAAAGTC CACTAAAGAT GAAACTAAGC TGAAACTCAC CTGTCTGGCC 
ACTGGCTTCT ACCCCAGCGA CGTGATGTTG AACATTAGGA GAAATCGCGT ATATCTGCCT 
GAACAAGAGA CCATATCTAC AGGAGTTCGA CCAAACCATG ATCAAACCTT CCAGCTGAGC 
AAGAGTGTGG AGATCAAGGA GGACCAGATA GATGAATATG ATTGTTCTGT GTCCCACAGA 
ACCTTCAAAC AACCAATTAT CATAAAATGG GATGGAACAG ATTTAGATGG AAAATACACC 
GGCTACCCAC CAGAGACTGT CCCAGTTGTG ATCGGAAGTG TGCTTATTTT ATTAGCCATT 
TTAGCTGTAG TGGTTTTTCT GGTGATGAAT TATGATGGTG AGTGTTGCAA AGGGCTCTTT 
ATTCACTTCA CCCTTCTTAA GGACTGTGGA CTGTTGCTGC CCCCTTGTGG AAAGACTGAG 
TATGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mhc1zka ( XM_017354485.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209974.1 Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa53485
Clone ID Related Accession (Same CDS sequence)	XM_017354485.2 , XM_017354485.1
Accession Version	XM_017354485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100536229 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336703.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTG AACAGCACCC TTATTACCCT CAACAAGAAA GGATGAACAG AGGCCCAAAG 
CCCACAATCC CTGACTTTAC TAAAGGAGAC CCACGGGAAT TTGCACGACT GAAAGTCTCA 
TTGGACAATC TTTTACCCGA GGATTCTACT GAAAGGTTTA AATATCAAAT ACTTCTAGAA 
CACTTGAAGT TTGAGGATGC TCTTTTCATT GCAGACTCCT ACATCAATTC CAGTAGACCG 
TATTCAGATA CCATGGCCTC TCTCGCAGAA CAATATGGGC AGCCCCACCA GCTAGCTTTG 
AGGCGAATTG CTGACTTAAT GGAAGATCCT TCCATACGTA GTCAAGATGC TAGTGGATTC 
AAGAGGTTTG CATTGAAAGT AAGAGCTCTA GTAGGCATGT TGAACCAATT GGGAGATAGT 
GGCAGAGTTG AGCTTCAGTG TGGCTCACAT GTAACACGGC TGTTGTCCAA GTTGCCCAAG 
TTGCCCCATG ACTTACGAGC AGATTTCAAA CGGTATGTGT ACCCCCTAAA TGTTCGCATC 
CCCACTCTGT TAAACTTTGC AGACTGGCTT GAATATGAGC TGAAAATACA GGCTAGTGGA 
TTTGAGTTTC TTGGTGGAGA CCGAAGGGAA CGTCCTGACC AGAGGAGAGA CAGGAGTAAA 
GACTTTAAGT CCACAAAGAC AGCAGCTGTA TTTCATAGCA CAGACCCTGC TTTAAATACT 
ATAGCTCCAG GAGAGTCCAG TTTGAGTGCT GCTAAAACTC AGGACAAACC AAGAATATTT 
TGCCCCTACT GCACAAATAC CCAGCATTAT CTGAATCAGT GTCAAAATTT TAGCCAGCTC 
ACCAAAGAAC AAAAAACAAA CTGGGTGAAA ACCAATAAGA GATGCTGGCG TTGTGGTCGA 
GCTCATCAGG CAGCACAGTG CAATTTAAAG ACAAGCTGTA AGGTCTGTAA AGGCAAACAT 
CTAGATGCAC TCCATGAGCT GAATGAAAGG CCAGTTTCTG AGAACACTTG TCTTGTCAAC 
AACGCCAATG AAGTTCTATA CCTGGACAGA CCATTAGGCT GCAGTCAAGT ACTGCTAAAG 
GTTACAAAGG TAATTCTTCG GAATGGGGAA CATACCCTTG AGACATATGC CATTTTGGAT 
GATGGCTCAG AACGCACAAT CCTTCTGCAA GCGGCCGCCC AGGAACTGAA GTTACAAGGC 
AAACCAGAAA ACCTTGCTCT GAGAACTATC AGACAGGACA TGAAAATCAT ACATGGTACT 
ACAGTCTCCT TTACCATCTC CCCTGCCTGC CAGCCTCACA AAGTCTTTAA GATACATAGA 
GCATTTACTG CAGACCATTT GGGGCTCGCT GAACACACAT GCCCAGCTAC CACCTTACAG 
CATAAATACA GACACCTCAG GGGACTGCCA ATCCCATCCC TTAACAGTGT TCAGCCCCTC 
CTTCTAATTG GTTCTGACTA CCCCTACCTA ATCACTCCCA TCGAGCCTGT ACGCCTAGGG 
CCTCCTGGCG GACCCGCAGC AGTGAAAACG CGACTAGGCT GGACAGTCCA GGGGCCTACT 
AAGCTTGTGC GGCATCAGCT TTTGACCCAG CAGTGTCTCA ATATATCCAT CTGTAGCCCT 
TCTAATGAGC TGTTCAGACA CATTGAGAAA CTCTGGCAAC TAGATGTACT GCCCTACAGA 
AGCGAGAAAC TTGCAACCAG ATCTAAGCAA GATCAAGAAG CTGTCGACCT GTTTGAGGCA 
AAGACAACTA GAATTGATGT CAATGGGACA CAACGATATG CCACACCTCT CTTAAGGGTT 
AAGGACATGC CAAAATTACA AGCTACTAAA CAAGCTGTAA TGCCGAATCT GCGCAGCACC 
GAAGCCCGCC TAGCCAAAGA TCCAATAAAG TCAGAGGCAT ATAGAGCTGA AATTCAAAGA 
CTGAAAGAAG CAGGTTATGT GGTTGAAGTA CCCGACCAGA AACTAACGAA GAAAGAGGAA 
GAATCATGGT TCATTCCCCA CCATATGGTG AGCCATAATG CCAAAAACAG AGTTATATTC 
AACTGCTCAT TCTCATATCA GGGTAAGAAC CTGAACAACC TGCTGTTGCC GGGACGAGCT 
CTGGGGTCCT CATTACTTGG AGTGTTGCTG CGCTTCAGGG AGCATGCTGT AGCCTTTAGT 
AGTGATATAA AGGGGATGTT TCATCAAGTA AGATTACTGC CCGAGGATAA GCCCCTACTC 
AGGTTCTTAT GGCGTGACCT AAAGAAGGAA GAGCCCCCCA GAGTCTATGA GTGGCAAGTG 
CTCCCATTCG GGACTACCTG CAGTCCGTGT TGTGCCACAT ACGCACTACA AAGGCACATT 
GTTGACCACA GTCAGCAAGG AGACAATGTC CGCCACTCAC TGGAAAGGTG TTTTTACGTA 
GATAATTGGC TACAGAGTGT TGCAACACCT GACGAAGCTA AAGAGCTGGT AGACACAGGG 
ATGAATCTGT TGGCTGAAGG AGGATTTGAA TTGCGTCAGT GGGCATGCAA TTTTCCGAAT 
GTCATTGAAC ATCTGTCAAA GGAAGCCAGA TCAGAAAGCA GTGAACGCTG GCTTAACCAA 
ACTGAATCCG ATCCTCAAGA ACTCGCTTTA GGATTACGAT GGATGTGCCA GTCTGATACT 
CTCGGATACC AAACCCGCCT TTACAACTGT TCCTCCCCCA CAATGAGAAA CATATACAAG 
GTAGTAGCAA GCCAGTATGA CCCTCTTGGA TTCCTTGTCC CCTACATCAC CCGAGCCAAG 
ATCCTTATAC AGAGTCTTTG GGCTAAGCAA CGAGAATGGG ATGACCCCCT ACCAATGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209974.1
CDS	1097..3919
Translation	MDPEQHPYYPQQERMNRGPKPTIPDFTKGDPREFARLKVSLDNLLPEDSTERFKYQILLEHLKFEDALFIADSYINSSRPYSDTMASLAEQYGQPHQLALRRIADLMEDPSIRSQDASGFKRFALKVRALVGMLNQLGDSGRVELQCGSHVTRLLSKLPKLPHDLRADFKRYVYPLNVRIPTLLNFADWLEYELKIQASGFEFLGGDRRERPDQRRDRSKDFKSTKTAAVFHSTDPALNTIAPGESSLSAAKTQDKPRIFCPYCTNTQHYLNQCQNFSQLTKEQKTNWVKTNKRCWRCGRAHQAAQCNLKTSCKVCKGKHLDALHELNERPVSENTCLVNNANEVLYLDRPLGCSQVLLKVTKVILRNGEHTLETYAILDDGSERTILLQAAAQELKLQGKPENLALRTIRQDMKIIHGTTVSFTISPACQPHKVFKIHRAFTADHLGLAEHTCPATTLQHKYRHLRGLPIPSLNSVQPLLLIGSDYPYLITPIEPVRLGPPGGPAAVKTRLGWTVQGPTKLVRHQLLTQQCLNISICSPSNELFRHIEKLWQLDVLPYRSEKLATRSKQDQEAVDLFEAKTTRIDVNGTQRYATPLLRVKDMPKLQATKQAVMPNLRSTEARLAKDPIKSEAYRAEIQRLKEAGYVVEVPDQKLTKKEEESWFIPHHMVSHNAKNRVIFNCSFSYQGKNLNNLLLPGRALGSSLLGVLLRFREHAVAFSSDIKGMFHQVRLLPEDKPLLRFLWRDLKKEEPPRVYEWQVLPFGTTCSPCCATYALQRHIVDHSQQGDNVRHSLERCFYVDNWLQSVATPDEAKELVDTGMNLLAEGGFELRQWACNFPNVIEHLSKEARSESSERWLNQTESDPQELALGLRWMCQSDTLGYQTRLYNCSSPTMRNIYKVVASQYDPLGFLVPYITRAKILIQSLWAKQREWDDPLPMG

Target ORF information:

RefSeq Version	XM_017354485.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354485.2

ORF Insert Sequence:

ATGGACCCTG AACAGCACCC TTATTACCCT CAACAAGAAA GGATGAACAG AGGCCCAAAG 
CCCACAATCC CTGACTTTAC TAAAGGAGAC CCACGGGAAT TTGCACGACT GAAAGTCTCA 
TTGGACAATC TTTTACCCGA GGATTCTACT GAAAGGTTTA AATATCAAAT ACTTCTAGAA 
CACTTGAAGT TTGAGGATGC TCTTTTCATT GCAGACTCCT ACATCAATTC CAGTAGACCG 
TATTCAGATA CCATGGCCTC TCTCGCAGAA CAATATGGGC AGCCCCACCA GCTAGCTTTG 
AGGCGAATTG CTGACTTAAT GGAAGATCCT TCCATACGTA GTCAAGATGC TAGTGGATTC 
AAGAGGTTTG CATTGAAAGT AAGAGCTCTA GTAGGCATGT TGAACCAATT GGGAGATAGT 
GGCAGAGTTG AGCTTCAGTG TGGCTCACAT GTAACACGGC TGTTGTCCAA GTTGCCCAAG 
TTGCCCCATG ACTTACGAGC AGATTTCAAA CGGTATGTGT ACCCCCTAAA TGTTCGCATC 
CCCACTCTGT TAAACTTTGC AGACTGGCTT GAATATGAGC TGAAAATACA GGCTAGTGGA 
TTTGAGTTTC TTGGTGGAGA CCGAAGGGAA CGTCCTGACC AGAGGAGAGA CAGGAGTAAA 
GACTTTAAGT CCACAAAGAC AGCAGCTGTA TTTCATAGCA CAGACCCTGC TTTAAATACT 
ATAGCTCCAG GAGAGTCCAG TTTGAGTGCT GCTAAAACTC AGGACAAACC AAGAATATTT 
TGCCCCTACT GCACAAATAC CCAGCATTAT CTGAATCAGT GTCAAAATTT TAGCCAGCTC 
ACCAAAGAAC AAAAAACAAA CTGGGTGAAA ACCAATAAGA GATGCTGGCG TTGTGGTCGA 
GCTCATCAGG CAGCACAGTG CAATTTAAAG ACAAGCTGTA AGGTCTGTAA AGGCAAACAT 
CTAGATGCAC TCCATGAGCT GAATGAAAGG CCAGTTTCTG AGAACACTTG TCTTGTCAAC 
AACGCCAATG AAGTTCTATA CCTGGACAGA CCATTAGGCT GCAGTCAAGT ACTGCTAAAG 
GTTACAAAGG TAATTCTTCG GAATGGGGAA CATACCCTTG AGACATATGC CATTTTGGAT 
GATGGCTCAG AACGCACAAT CCTTCTGCAA GCGGCCGCCC AGGAACTGAA GTTACAAGGC 
AAACCAGAAA ACCTTGCTCT GAGAACTATC AGACAGGACA TGAAAATCAT ACATGGTACT 
ACAGTCTCCT TTACCATCTC CCCTGCCTGC CAGCCTCACA AAGTCTTTAA GATACATAGA 
GCATTTACTG CAGACCATTT GGGGCTCGCT GAACACACAT GCCCAGCTAC CACCTTACAG 
CATAAATACA GACACCTCAG GGGACTGCCA ATCCCATCCC TTAACAGTGT TCAGCCCCTC 
CTTCTAATTG GTTCTGACTA CCCCTACCTA ATCACTCCCA TCGAGCCTGT ACGCCTAGGG 
CCTCCTGGCG GACCCGCAGC AGTGAAAACG CGACTAGGCT GGACAGTCCA GGGGCCTACT 
AAGCTTGTGC GGCATCAGCT TTTGACCCAG CAGTGTCTCA ATATATCCAT CTGTAGCCCT 
TCTAATGAGC TGTTCAGACA CATTGAGAAA CTCTGGCAAC TAGATGTACT GCCCTACAGA 
AGCGAGAAAC TTGCAACCAG ATCTAAGCAA GATCAAGAAG CTGTCGACCT GTTTGAGGCA 
AAGACAACTA GAATTGATGT CAATGGGACA CAACGATATG CCACACCTCT CTTAAGGGTT 
AAGGACATGC CAAAATTACA AGCTACTAAA CAAGCTGTAA TGCCGAATCT GCGCAGCACC 
GAAGCCCGCC TAGCCAAAGA TCCAATAAAG TCAGAGGCAT ATAGAGCTGA AATTCAAAGA 
CTGAAAGAAG CAGGTTATGT GGTTGAAGTA CCCGACCAGA AACTAACGAA GAAAGAGGAA 
GAATCATGGT TCATTCCCCA CCATATGGTG AGCCATAATG CCAAAAACAG AGTTATATTC 
AACTGCTCAT TCTCATATCA GGGTAAGAAC CTGAACAACC TGCTGTTGCC GGGACGAGCT 
CTGGGGTCCT CATTACTTGG AGTGTTGCTG CGCTTCAGGG AGCATGCTGT AGCCTTTAGT 
AGTGATATAA AGGGGATGTT TCATCAAGTA AGATTACTGC CCGAGGATAA GCCCCTACTC 
AGGTTCTTAT GGCGTGACCT AAAGAAGGAA GAGCCCCCCA GAGTCTATGA GTGGCAAGTG 
CTCCCATTCG GGACTACCTG CAGTCCGTGT TGTGCCACAT ACGCACTACA AAGGCACATT 
GTTGACCACA GTCAGCAAGG AGACAATGTC CGCCACTCAC TGGAAAGGTG TTTTTACGTA 
GATAATTGGC TACAGAGTGT TGCAACACCT GACGAAGCTA AAGAGCTGGT AGACACAGGG 
ATGAATCTGT TGGCTGAAGG AGGATTTGAA TTGCGTCAGT GGGCATGCAA TTTTCCGAAT 
GTCATTGAAC ATCTGTCAAA GGAAGCCAGA TCAGAAAGCA GTGAACGCTG GCTTAACCAA 
ACTGAATCCG ATCCTCAAGA ACTCGCTTTA GGATTACGAT GGATGTGCCA GTCTGATACT 
CTCGGATACC AAACCCGCCT TTACAACTGT TCCTCCCCCA CAATGAGAAA CATATACAAG 
GTAGTAGCAA GCCAGTATGA CCCTCTTGGA TTCCTTGTCC CCTACATCAC CCGAGCCAAG 
ATCCTTATAC AGAGTCTTTG GGCTAAGCAA CGAGAATGGG ATGACCCCCT ACCAATGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mhc1zka ( XM_017354485.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209974.1 Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa53485
Clone ID Related Accession (Same CDS sequence)	XM_017354485.2 , XM_017354485.1
Accession Version	XM_017354485.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100536229 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003336703.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTG AACAGCACCC TTATTACCCT CAACAAGAAA GGATGAACAG AGGCCCAAAG 
CCCACAATCC CTGACTTTAC TAAAGGAGAC CCACGGGAAT TTGCACGACT GAAAGTCTCA 
TTGGACAATC TTTTACCCGA GGATTCTACT GAAAGGTTTA AATATCAAAT ACTTCTAGAA 
CACTTGAAGT TTGAGGATGC TCTTTTCATT GCAGACTCCT ACATCAATTC CAGTAGACCG 
TATTCAGATA CCATGGCCTC TCTCGCAGAA CAATATGGGC AGCCCCACCA GCTAGCTTTG 
AGGCGAATTG CTGACTTAAT GGAAGATCCT TCCATACGTA GTCAAGATGC TAGTGGATTC 
AAGAGGTTTG CATTGAAAGT AAGAGCTCTA GTAGGCATGT TGAACCAATT GGGAGATAGT 
GGCAGAGTTG AGCTTCAGTG TGGCTCACAT GTAACACGGC TGTTGTCCAA GTTGCCCAAG 
TTGCCCCATG ACTTACGAGC AGATTTCAAA CGGTATGTGT ACCCCCTAAA TGTTCGCATC 
CCCACTCTGT TAAACTTTGC AGACTGGCTT GAATATGAGC TGAAAATACA GGCTAGTGGA 
TTTGAGTTTC TTGGTGGAGA CCGAAGGGAA CGTCCTGACC AGAGGAGAGA CAGGAGTAAA 
GACTTTAAGT CCACAAAGAC AGCAGCTGTA TTTCATAGCA CAGACCCTGC TTTAAATACT 
ATAGCTCCAG GAGAGTCCAG TTTGAGTGCT GCTAAAACTC AGGACAAACC AAGAATATTT 
TGCCCCTACT GCACAAATAC CCAGCATTAT CTGAATCAGT GTCAAAATTT TAGCCAGCTC 
ACCAAAGAAC AAAAAACAAA CTGGGTGAAA ACCAATAAGA GATGCTGGCG TTGTGGTCGA 
GCTCATCAGG CAGCACAGTG CAATTTAAAG ACAAGCTGTA AGGTCTGTAA AGGCAAACAT 
CTAGATGCAC TCCATGAGCT GAATGAAAGG CCAGTTTCTG AGAACACTTG TCTTGTCAAC 
AACGCCAATG AAGTTCTATA CCTGGACAGA CCATTAGGCT GCAGTCAAGT ACTGCTAAAG 
GTTACAAAGG TAATTCTTCG GAATGGGGAA CATACCCTTG AGACATATGC CATTTTGGAT 
GATGGCTCAG AACGCACAAT CCTTCTGCAA GCGGCCGCCC AGGAACTGAA GTTACAAGGC 
AAACCAGAAA ACCTTGCTCT GAGAACTATC AGACAGGACA TGAAAATCAT ACATGGTACT 
ACAGTCTCCT TTACCATCTC CCCTGCCTGC CAGCCTCACA AAGTCTTTAA GATACATAGA 
GCATTTACTG CAGACCATTT GGGGCTCGCT GAACACACAT GCCCAGCTAC CACCTTACAG 
CATAAATACA GACACCTCAG GGGACTGCCA ATCCCATCCC TTAACAGTGT TCAGCCCCTC 
CTTCTAATTG GTTCTGACTA CCCCTACCTA ATCACTCCCA TCGAGCCTGT ACGCCTAGGG 
CCTCCTGGCG GACCCGCAGC AGTGAAAACG CGACTAGGCT GGACAGTCCA GGGGCCTACT 
AAGCTTGTGC GGCATCAGCT TTTGACCCAG CAGTGTCTCA ATATATCCAT CTGTAGCCCT 
TCTAATGAGC TGTTCAGACA CATTGAGAAA CTCTGGCAAC TAGATGTACT GCCCTACAGA 
AGCGAGAAAC TTGCAACCAG ATCTAAGCAA GATCAAGAAG CTGTCGACCT GTTTGAGGCA 
AAGACAACTA GAATTGATGT CAATGGGACA CAACGATATG CCACACCTCT CTTAAGGGTT 
AAGGACATGC CAAAATTACA AGCTACTAAA CAAGCTGTAA TGCCGAATCT GCGCAGCACC 
GAAGCCCGCC TAGCCAAAGA TCCAATAAAG TCAGAGGCAT ATAGAGCTGA AATTCAAAGA 
CTGAAAGAAG CAGGTTATGT GGTTGAAGTA CCCGACCAGA AACTAACGAA GAAAGAGGAA 
GAATCATGGT TCATTCCCCA CCATATGGTG AGCCATAATG CCAAAAACAG AGTTATATTC 
AACTGCTCAT TCTCATATCA GGGTAAGAAC CTGAACAACC TGCTGTTGCC GGGACGAGCT 
CTGGGGTCCT CATTACTTGG AGTGTTGCTG CGCTTCAGGG AGCATGCTGT AGCCTTTAGT 
AGTGATATAA AGGGGATGTT TCATCAAGTA AGATTACTGC CCGAGGATAA GCCCCTACTC 
AGGTTCTTAT GGCGTGACCT AAAGAAGGAA GAGCCCCCCA GAGTCTATGA GTGGCAAGTG 
CTCCCATTCG GGACTACCTG CAGTCCGTGT TGTGCCACAT ACGCACTACA AAGGCACATT 
GTTGACCACA GTCAGCAAGG AGACAATGTC CGCCACTCAC TGGAAAGGTG TTTTTACGTA 
GATAATTGGC TACAGAGTGT TGCAACACCT GACGAAGCTA AAGAGCTGGT AGACACAGGG 
ATGAATCTGT TGGCTGAAGG AGGATTTGAA TTGCGTCAGT GGGCATGCAA TTTTCCGAAT 
GTCATTGAAC ATCTGTCAAA GGAAGCCAGA TCAGAAAGCA GTGAACGCTG GCTTAACCAA 
ACTGAATCCG ATCCTCAAGA ACTCGCTTTA GGATTACGAT GGATGTGCCA GTCTGATACT 
CTCGGATACC AAACCCGCCT TTACAACTGT TCCTCCCCCA CAATGAGAAA CATATACAAG 
GTAGTAGCAA GCCAGTATGA CCCTCTTGGA TTCCTTGTCC CCTACATCAC CCGAGCCAAG 
ATCCTTATAC AGAGTCTTTG GGCTAAGCAA CGAGAATGGG ATGACCCCCT ACCAATGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209974.1
CDS	1093..3915
Translation	MDPEQHPYYPQQERMNRGPKPTIPDFTKGDPREFARLKVSLDNLLPEDSTERFKYQILLEHLKFEDALFIADSYINSSRPYSDTMASLAEQYGQPHQLALRRIADLMEDPSIRSQDASGFKRFALKVRALVGMLNQLGDSGRVELQCGSHVTRLLSKLPKLPHDLRADFKRYVYPLNVRIPTLLNFADWLEYELKIQASGFEFLGGDRRERPDQRRDRSKDFKSTKTAAVFHSTDPALNTIAPGESSLSAAKTQDKPRIFCPYCTNTQHYLNQCQNFSQLTKEQKTNWVKTNKRCWRCGRAHQAAQCNLKTSCKVCKGKHLDALHELNERPVSENTCLVNNANEVLYLDRPLGCSQVLLKVTKVILRNGEHTLETYAILDDGSERTILLQAAAQELKLQGKPENLALRTIRQDMKIIHGTTVSFTISPACQPHKVFKIHRAFTADHLGLAEHTCPATTLQHKYRHLRGLPIPSLNSVQPLLLIGSDYPYLITPIEPVRLGPPGGPAAVKTRLGWTVQGPTKLVRHQLLTQQCLNISICSPSNELFRHIEKLWQLDVLPYRSEKLATRSKQDQEAVDLFEAKTTRIDVNGTQRYATPLLRVKDMPKLQATKQAVMPNLRSTEARLAKDPIKSEAYRAEIQRLKEAGYVVEVPDQKLTKKEEESWFIPHHMVSHNAKNRVIFNCSFSYQGKNLNNLLLPGRALGSSLLGVLLRFREHAVAFSSDIKGMFHQVRLLPEDKPLLRFLWRDLKKEEPPRVYEWQVLPFGTTCSPCCATYALQRHIVDHSQQGDNVRHSLERCFYVDNWLQSVATPDEAKELVDTGMNLLAEGGFELRQWACNFPNVIEHLSKEARSESSERWLNQTESDPQELALGLRWMCQSDTLGYQTRLYNCSSPTMRNIYKVVASQYDPLGFLVPYITRAKILIQSLWAKQREWDDPLPMG

Target ORF information:

RefSeq Version	XM_017354485.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio major histocompatibility complex class I ZKA (mhc1zka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354485.1

ORF Insert Sequence:

ATGGACCCTG AACAGCACCC TTATTACCCT CAACAAGAAA GGATGAACAG AGGCCCAAAG 
CCCACAATCC CTGACTTTAC TAAAGGAGAC CCACGGGAAT TTGCACGACT GAAAGTCTCA 
TTGGACAATC TTTTACCCGA GGATTCTACT GAAAGGTTTA AATATCAAAT ACTTCTAGAA 
CACTTGAAGT TTGAGGATGC TCTTTTCATT GCAGACTCCT ACATCAATTC CAGTAGACCG 
TATTCAGATA CCATGGCCTC TCTCGCAGAA CAATATGGGC AGCCCCACCA GCTAGCTTTG 
AGGCGAATTG CTGACTTAAT GGAAGATCCT TCCATACGTA GTCAAGATGC TAGTGGATTC 
AAGAGGTTTG CATTGAAAGT AAGAGCTCTA GTAGGCATGT TGAACCAATT GGGAGATAGT 
GGCAGAGTTG AGCTTCAGTG TGGCTCACAT GTAACACGGC TGTTGTCCAA GTTGCCCAAG 
TTGCCCCATG ACTTACGAGC AGATTTCAAA CGGTATGTGT ACCCCCTAAA TGTTCGCATC 
CCCACTCTGT TAAACTTTGC AGACTGGCTT GAATATGAGC TGAAAATACA GGCTAGTGGA 
TTTGAGTTTC TTGGTGGAGA CCGAAGGGAA CGTCCTGACC AGAGGAGAGA CAGGAGTAAA 
GACTTTAAGT CCACAAAGAC AGCAGCTGTA TTTCATAGCA CAGACCCTGC TTTAAATACT 
ATAGCTCCAG GAGAGTCCAG TTTGAGTGCT GCTAAAACTC AGGACAAACC AAGAATATTT 
TGCCCCTACT GCACAAATAC CCAGCATTAT CTGAATCAGT GTCAAAATTT TAGCCAGCTC 
ACCAAAGAAC AAAAAACAAA CTGGGTGAAA ACCAATAAGA GATGCTGGCG TTGTGGTCGA 
GCTCATCAGG CAGCACAGTG CAATTTAAAG ACAAGCTGTA AGGTCTGTAA AGGCAAACAT 
CTAGATGCAC TCCATGAGCT GAATGAAAGG CCAGTTTCTG AGAACACTTG TCTTGTCAAC 
AACGCCAATG AAGTTCTATA CCTGGACAGA CCATTAGGCT GCAGTCAAGT ACTGCTAAAG 
GTTACAAAGG TAATTCTTCG GAATGGGGAA CATACCCTTG AGACATATGC CATTTTGGAT 
GATGGCTCAG AACGCACAAT CCTTCTGCAA GCGGCCGCCC AGGAACTGAA GTTACAAGGC 
AAACCAGAAA ACCTTGCTCT GAGAACTATC AGACAGGACA TGAAAATCAT ACATGGTACT 
ACAGTCTCCT TTACCATCTC CCCTGCCTGC CAGCCTCACA AAGTCTTTAA GATACATAGA 
GCATTTACTG CAGACCATTT GGGGCTCGCT GAACACACAT GCCCAGCTAC CACCTTACAG 
CATAAATACA GACACCTCAG GGGACTGCCA ATCCCATCCC TTAACAGTGT TCAGCCCCTC 
CTTCTAATTG GTTCTGACTA CCCCTACCTA ATCACTCCCA TCGAGCCTGT ACGCCTAGGG 
CCTCCTGGCG GACCCGCAGC AGTGAAAACG CGACTAGGCT GGACAGTCCA GGGGCCTACT 
AAGCTTGTGC GGCATCAGCT TTTGACCCAG CAGTGTCTCA ATATATCCAT CTGTAGCCCT 
TCTAATGAGC TGTTCAGACA CATTGAGAAA CTCTGGCAAC TAGATGTACT GCCCTACAGA 
AGCGAGAAAC TTGCAACCAG ATCTAAGCAA GATCAAGAAG CTGTCGACCT GTTTGAGGCA 
AAGACAACTA GAATTGATGT CAATGGGACA CAACGATATG CCACACCTCT CTTAAGGGTT 
AAGGACATGC CAAAATTACA AGCTACTAAA CAAGCTGTAA TGCCGAATCT GCGCAGCACC 
GAAGCCCGCC TAGCCAAAGA TCCAATAAAG TCAGAGGCAT ATAGAGCTGA AATTCAAAGA 
CTGAAAGAAG CAGGTTATGT GGTTGAAGTA CCCGACCAGA AACTAACGAA GAAAGAGGAA 
GAATCATGGT TCATTCCCCA CCATATGGTG AGCCATAATG CCAAAAACAG AGTTATATTC 
AACTGCTCAT TCTCATATCA GGGTAAGAAC CTGAACAACC TGCTGTTGCC GGGACGAGCT 
CTGGGGTCCT CATTACTTGG AGTGTTGCTG CGCTTCAGGG AGCATGCTGT AGCCTTTAGT 
AGTGATATAA AGGGGATGTT TCATCAAGTA AGATTACTGC CCGAGGATAA GCCCCTACTC 
AGGTTCTTAT GGCGTGACCT AAAGAAGGAA GAGCCCCCCA GAGTCTATGA GTGGCAAGTG 
CTCCCATTCG GGACTACCTG CAGTCCGTGT TGTGCCACAT ACGCACTACA AAGGCACATT 
GTTGACCACA GTCAGCAAGG AGACAATGTC CGCCACTCAC TGGAAAGGTG TTTTTACGTA 
GATAATTGGC TACAGAGTGT TGCAACACCT GACGAAGCTA AAGAGCTGGT AGACACAGGG 
ATGAATCTGT TGGCTGAAGG AGGATTTGAA TTGCGTCAGT GGGCATGCAA TTTTCCGAAT 
GTCATTGAAC ATCTGTCAAA GGAAGCCAGA TCAGAAAGCA GTGAACGCTG GCTTAACCAA 
ACTGAATCCG ATCCTCAAGA ACTCGCTTTA GGATTACGAT GGATGTGCCA GTCTGATACT 
CTCGGATACC AAACCCGCCT TTACAACTGT TCCTCCCCCA CAATGAGAAA CATATACAAG 
GTAGTAGCAA GCCAGTATGA CCCTCTTGGA TTCCTTGTCC CCTACATCAC CCGAGCCAAG 
ATCCTTATAC AGAGTCTTTG GGCTAAGCAA CGAGAATGGG ATGACCCCCT ACCAATGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways.
Frontiers in immunology9726(2018)
Wu XM,Chen WQ,Hu YW,Cao L,Nie P,Chang MX

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The identification of additional zebrafish DICP genes reveals haplotype variation and linkage to MHC class I genes.
Immunogenetics68(4)295-312(2016 Apr)
Rodriguez-Nunez I,Wcisel DJ,Litman RT,Litman GW,Yoder JA

A nonclassical MHC class I U lineage locus in zebrafish with a null haplotypic variant.
Immunogenetics67(9)501-13(2015 Sep)
Dirscherl H,Yoder JA

The MHC class I genes of zebrafish.
Developmental and comparative immunology46(1)11-23(2014 Sep)
Dirscherl H,McConnell SC,Yoder JA,de Jong JL

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mhc1zka cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

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ORF Insert Sequence:

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