rps27.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rps27.1
Entrez Gene ID	554155
Full Name	ribosomal protein S27, isoform 1
Synonyms	wu:fa18f12,zgc:109888
General protein information	Preferred Names ribosomal protein S27, isoform 1 Names 40S ribosomal protein S27-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159528.1	NP_001153000.1	40S ribosomal protein S27-like

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_02872	XP_003720862.1
Rattus norvegicus (Norway rat)	Rps27	NP_446049.1
Danio rerio (zebrafish)	rps27.2	NP_957059.1
Drosophila melanogaster (fruit fly)	RpS27	NP_651359.1
Caenorhabditis elegans (roundworm)	rps-27	NP_503134.1
Saccharomyces cerevisiae (baker's yeast)	RPS27B	NP_011885.1
Saccharomyces cerevisiae (baker's yeast)	RPS27A	NP_012766.1
Xenopus tropicalis (tropical clawed frog)	rps27	NP_001165123.1
Pan troglodytes (chimpanzee)	LOC100614845	XP_003312546.1
Homo sapiens (human)	RPS27	NP_001021.1
Pan troglodytes (chimpanzee)	LOC452745	XP_509802.2
Danio rerio (zebrafish)	rps27.1	NP_001153000.1
Arabidopsis thaliana (thale cress)	RS27A	NP_191670.1
Kluyveromyces lactis	KLLA0E11661g	XP_454477.1
Schizosaccharomyces pombe (fission yeast)	rps27	NP_595214.1
Oryza sativa (rice)	Os02g0478700	NP_001046847.1
Oryza sativa (rice)	Os04g0346100	NP_001052512.1
Arabidopsis thaliana (thale cress)	AT3G61111	NP_001118871.1
Pan troglodytes (chimpanzee)	RPS27	NP_001238926.1
Bos taurus (cattle)	RPS27	NP_001091604.1
Arabidopsis thaliana (thale cress)	AT5G47930	NP_199604.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007157	XP_308611.1
Canis lupus familiaris (dog)	RPS27	NP_001239215.1
Arabidopsis thaliana (thale cress)	AT2G45710	NP_182095.1
Eremothecium gossypii	AGOS_ACR065C	NP_983467.2
Neurospora crassa	NCU00618	XP_965758.1

Gene Ontology
Bibliography

Related articles in PubMed

Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis.
He WX, Wu M, Liu Z, Li Z, Wang Y, Zhou J, Yu P, Zhang XJ, Zhou L, Gui JF
RNA (New York, N.Y.)24(12)1738-1748(2018 12)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia.
Wang R, Yoshida K, Toki T, Sawada T, Uechi T, Okuno Y, Sato-Otsubo A, Kudo K, Kamimaki I, Kanezaki R, Shiraishi Y, Chiba K, Tanaka H, Terui K, Sato T, Iribe Y, Ohga S, Kuramitsu M, Hamaguchi I, Ohara A, Hara J, Goi K, Matsubara K, Koike K, Ishiguro A, Okamoto Y, Watanabe K, Kanno H, Kojima S, Miyano S, Kenmochi N, Ogawa S, Ito E
British journal of haematology168(6)854-64(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rps27.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rps27.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35961	NM_001159528.1 Latest version!	Danio rerio ribosomal protein S27, isoform 1 (rps27.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rps27.1 ( NM_001159528.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001153000.1 Danio rerio ribosomal protein S27, isoform 1 (rps27.1), mRNA.

Price & Availability↑

CloneID	ODa35961
Clone ID Related Accession (Same CDS sequence)	NM_001159528.1
Accession Version	NM_001159528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553875200000
Organism	Danio rerio(zebrafish)
Product	40S ribosomal protein S27-like
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EB867690.1, BC095031.1 and BM159046.1. On Apr 19, 2009 this sequence version replaced XM_678730.3. ##Evidence-Data-START## Transcript exon combination :: EH540509.1, CN023448.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGCCACTCG CAAAAGACTT GCTGCACCCG ACCCCTGAAG AAGAGAGGAG GAGGCACAAG 
AAGAAGCGTC TCGTACAGAG CCCCAACTCT TATTTCATGG ATGTCAAATG TCCAGGATGT 
TATAAGATCA CAACGGTGTT CAGCCATGCA CAGACAGTGG TGCTTTGTGT CGGTTGCTCG 
ACTGTGCTGT GTCAGCCCAC TGGAGGCAAA GCTCGCCTCA CAGAAGGGTG TTCCTTCAGA 
AGGAAGCAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153000.1
CDS	43..297
Translation	MPLAKDLLHPTPEEERRRHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH

Target ORF information:

RefSeq Version	NM_001159528.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ribosomal protein S27, isoform 1 (rps27.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159528.1

ORF Insert Sequence:

ATGCCACTCG CAAAAGACTT GCTGCACCCG ACCCCTGAAG AAGAGAGGAG GAGGCACAAG 
AAGAAGCGTC TCGTACAGAG CCCCAACTCT TATTTCATGG ATGTCAAATG TCCAGGATGT 
TATAAGATCA CAACGGTGTT CAGCCATGCA CAGACAGTGG TGCTTTGTGT CGGTTGCTCG 
ACTGTGCTGT GTCAGCCCAC TGGAGGCAAA GCTCGCCTCA CAGAAGGGTG TTCCTTCAGA 
AGGAAGCAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis.
RNA (New York, N.Y.)24(12)1738-1748(2018 12)
He WX,Wu M,Liu Z,Li Z,Wang Y,Zhou J,Yu P,Zhang XJ,Zhou L,Gui JF

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia.
British journal of haematology168(6)854-64(2015 Mar)
Wang R,Yoshida K,Toki T,Sawada T,Uechi T,Okuno Y,Sato-Otsubo A,Kudo K,Kamimaki I,Kanezaki R,Shiraishi Y,Chiba K,Tanaka H,Terui K,Sato T,Iribe Y,Ohga S,Kuramitsu M,Hamaguchi I,Ohara A,Hara J,Goi K,Matsubara K,Koike K,Ishiguro A,Okamoto Y,Watanabe K,Kanno H,Kojima S,Miyano S,Kenmochi N,Ogawa S,Ito E

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rps27.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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