ppm1e cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppm1e
Entrez Gene ID	553417
Full Name	protein phosphatase, Mg2+/Mn2+ dependent, 1E
General protein information	Preferred Names protein phosphatase, Mg2+/Mn2+ dependent, 1E Names protein phosphatase 1E Ca/calmodulin-dependent protein kinase phosphatase-N camkp-n protein phosphatase 1E (PP2C domain containing)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020518.1	NP_001018354.1	protein phosphatase 1E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppm1e	NP_942068.1
Canis lupus familiaris (dog)	PPM1E	XP_853253.1
Gallus gallus (chicken)	PPM1E	XP_415871.4
Homo sapiens (human)	PPM1E	NP_055721.3
Danio rerio (zebrafish)	ppm1e	NP_001018354.1
Pan troglodytes (chimpanzee)	PPM1E	XP_523813.4
Macaca mulatta (Rhesus monkey)	LOC711012	XP_002800484.1
Bos taurus (cattle)	PPM1E	XP_002695648.3
Mus musculus (house mouse)	Ppm1e	NP_796141.2
Xenopus tropicalis (tropical clawed frog)	ppm1e	XP_002934392.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Emerson SE, St Clair RM, Waldron AL, Bruno SR, Duong A, Driscoll HE, Ballif BA, McFarlane S, Ebert AM
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 07)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phosphorylation and activation of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N/PPM1E) by Ca2+/calmodulin-dependent protein kinase I (CaMKI).
Onouchi T, Sueyoshi N, Ishida A, Kameshita I
Biochemical and biophysical research communications422(4)703-9(2012 Jun)

Functional processing of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N): evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, subcellular localization and substrate targeting in vivo.
Sueyoshi N, Nimura T, Onouchi T, Baba H, Takenaka S, Ishida A, Kameshita I
Archives of biochemistry and biophysics517(1)43-52(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ppm1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppm1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03217	NM_001020518.1 Latest version!	Danio rerio protein phosphatase, Mg2+/Mn2+ dependent, 1E (ppm1e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppm1e ( NM_001020518.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018354.1 Danio rerio protein phosphatase, Mg2+/Mn2+ dependent, 1E (ppm1e), mRNA.

Price & Availability↑

CloneID	ODa03217
Clone ID Related Accession (Same CDS sequence)	NM_001020518.1
Accession Version	NM_001020518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	protein phosphatase 1E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB113302.1. ##Evidence-Data-START## Transcript exon combination :: AB113302.1, BC162507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505379 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGCT CTGCCAACGA GGAGAAAACC TTCAAGAGGT TCCTGGAGCT CTTCTTACGG 
GAGATGAGGC CACCTATCCA GGAGGACGTA CCTCTGCCTA CTCGCCCCTT GTCGGACCTC 
ATCTCGGAGG ACGAGGTGGA GGGGGAATGC CTGGACCTGT GTCTCCAGCA CCTGTGTAAA 
TATAATTGCC CTTTCTCCTT GGCTGCAGCT CTGGCCCGCG GTACAGCAGA CAGCATCCTG 
CAAAGTGATC TCTCCTTGTA CCATCTCAAC AAGAGTGTTG AGGACGGGAC TGAGACTGAG 
ACTGAGACAG AAACGGAGAC ACAGACCCAG ATCGATTCGG TGAAGCTGGC GCGACTTGTC 
TTCAATAAGC TGTGTGAGAC GTGCAGTCAA TGGCTAAAAG ACTTTCCATA TCGCCGCCGT 
CACTTGCCCT ACTATGAGAC TTCCATCCAT GCTATTAAAA ACATGCGCCG GAAGATGGAG 
GACAAACACG TTGTGATTCC TGACTTCAAC ACACTCTTCA ATCTACAGGA TCAAGAAGAG 
CAGGCCTACT TTGCCGTGTT TGATGGTCAC GGCGGGGTGG ACGCTGCCAA CTACGCCGCC 
AATCACCTGC ATGTTAATCT GGTCAGGCAA GAAATGTTCA GCCAGGACGC AGGAGAAGCC 
TTGTGTCACT CCTTCAAACT GACCGATGAG AGATTCATCA AAAAAGCAAA AAGCGAGAAC 
CTGCGCTGTG GCACCACAGG GGTCGTGACT TTTCTGAGGG GTCGCACGCT GTACGTGACC 
TGGCTGGGCG ATTCACAGGT CATGATGGTG AAGAGAGGTC AACCGGTGGA GCTGATGAAG 
CCACACAAAC CTGACAGAGA GGATGAGAAA AAGAGGATTG AGGCATTGGG AGGGTGTGTG 
ATCTGGTTCG GCACATGGAG GGTGAATGGC AGCCTGTCTG TGTCAAGAGC CATCGGTGAT 
TCAGAACACA AGCCCTACAT CTGTGGCGAT GCTGACTGCA GCACCTTCAA CCTGGACGGC 
AGCGAGGACT ACTTGATCCT GGCCTGCGAC GGCTTCTACG ACACAGTGAA CCCAGAAGAA 
GCCGTCCGAG TGGTGAGCGA CCACTTGCAG GAGAACAACG GGGACACCGC CATGGTGGCC 
CACAAGCTGG TGGCCTCAGC CCGAGATGCG GGGTCCAGTG ACAACATCAC GGTAATCGTA 
GTGTTCCTCA GAGACCCCCG TTTGCCTCCG CCCTCAGATG AGCCTGAGGA GCAGCAGGAT 
GAGGAGCCAT TGGAGGACGC AGGAGAAGAG GAAGAAGAGG AAGAAGCAGT GGATGCCTTG 
CAGAGTGAAT TGGTGTGTCA AGATGGAGGT GCGGAGAATG GAGGAAAGAA CCGGGGCGGC 
TGGCCTCTGC AGCAGTGCTC AGCGCCAGCT GACCTGGCCT ACGAAGACCG CATGGATTCA 
TTCACGGACA GAACAAGTCT GAGCATCACA GGGCCGGATG TGCATGCAGA GACTGGTTGC 
TTCAGGATCC CAGCTTCATC TGGCCTGCCC CCAACCAGAA CCGTCACGCC AGACCTCATT 
CCCTCCAACA GCAGGGCCGG CGCTGCCTGG CGTCCTTACC AAGAGGACGA CCCTTCTGGG 
TACAGGCAAA AACCCCAGCC TAATCCTAGA GTGCCCTCCT ACATGCAGAC CACCTCAGAT 
GGACAATTGC TGGAGGTGGC TGCGCTCTTC TCTCAGGAGG GCCGCAAGAA GAGGCGGCTG 
GACGTCCTGC CGCTGAGGAG GGAATCACAG CGCCTGACCC GGAGGGCCAG GGAAAGCCAC 
GGCCCTCTGA GCTGCTTCCC TAGTGTGCCC TATCCTCTGC GCTCCTCAGA GAGGAAGCCC 
GGGATAGCCT TTCCTCATGT AACCCATAGC AAAAGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018354.1
CDS	57..1958
Translation	MAGSANEEKTFKRFLELFLREMRPPIQEDVPLPTRPLSDLISEDEVEGECLDLCLQHLCKYNCPFSLAAALARGTADSILQSDLSLYHLNKSVEDGTETETETETETQTQIDSVKLARLVFNKLCETCSQWLKDFPYRRRHLPYYETSIHAIKNMRRKMEDKHVVIPDFNTLFNLQDQEEQAYFAVFDGHGGVDAANYAANHLHVNLVRQEMFSQDAGEALCHSFKLTDERFIKKAKSENLRCGTTGVVTFLRGRTLYVTWLGDSQVMMVKRGQPVELMKPHKPDREDEKKRIEALGGCVIWFGTWRVNGSLSVSRAIGDSEHKPYICGDADCSTFNLDGSEDYLILACDGFYDTVNPEEAVRVVSDHLQENNGDTAMVAHKLVASARDAGSSDNITVIVVFLRDPRLPPPSDEPEEQQDEEPLEDAGEEEEEEEAVDALQSELVCQDGGAENGGKNRGGWPLQQCSAPADLAYEDRMDSFTDRTSLSITGPDVHAETGCFRIPASSGLPPTRTVTPDLIPSNSRAGAAWRPYQEDDPSGYRQKPQPNPRVPSYMQTTSDGQLLEVAALFSQEGRKKRRLDVLPLRRESQRLTRRARESHGPLSCFPSVPYPLRSSERKPGIAFPHVTHSKRV

Target ORF information:

RefSeq Version	NM_001020518.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein phosphatase, Mg2+/Mn2+ dependent, 1E (ppm1e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020518.1

ORF Insert Sequence:

ATGGCCGGCT CTGCCAACGA GGAGAAAACC TTCAAGAGGT TCCTGGAGCT CTTCTTACGG 
GAGATGAGGC CACCTATCCA GGAGGACGTA CCTCTGCCTA CTCGCCCCTT GTCGGACCTC 
ATCTCGGAGG ACGAGGTGGA GGGGGAATGC CTGGACCTGT GTCTCCAGCA CCTGTGTAAA 
TATAATTGCC CTTTCTCCTT GGCTGCAGCT CTGGCCCGCG GTACAGCAGA CAGCATCCTG 
CAAAGTGATC TCTCCTTGTA CCATCTCAAC AAGAGTGTTG AGGACGGGAC TGAGACTGAG 
ACTGAGACAG AAACGGAGAC ACAGACCCAG ATCGATTCGG TGAAGCTGGC GCGACTTGTC 
TTCAATAAGC TGTGTGAGAC GTGCAGTCAA TGGCTAAAAG ACTTTCCATA TCGCCGCCGT 
CACTTGCCCT ACTATGAGAC TTCCATCCAT GCTATTAAAA ACATGCGCCG GAAGATGGAG 
GACAAACACG TTGTGATTCC TGACTTCAAC ACACTCTTCA ATCTACAGGA TCAAGAAGAG 
CAGGCCTACT TTGCCGTGTT TGATGGTCAC GGCGGGGTGG ACGCTGCCAA CTACGCCGCC 
AATCACCTGC ATGTTAATCT GGTCAGGCAA GAAATGTTCA GCCAGGACGC AGGAGAAGCC 
TTGTGTCACT CCTTCAAACT GACCGATGAG AGATTCATCA AAAAAGCAAA AAGCGAGAAC 
CTGCGCTGTG GCACCACAGG GGTCGTGACT TTTCTGAGGG GTCGCACGCT GTACGTGACC 
TGGCTGGGCG ATTCACAGGT CATGATGGTG AAGAGAGGTC AACCGGTGGA GCTGATGAAG 
CCACACAAAC CTGACAGAGA GGATGAGAAA AAGAGGATTG AGGCATTGGG AGGGTGTGTG 
ATCTGGTTCG GCACATGGAG GGTGAATGGC AGCCTGTCTG TGTCAAGAGC CATCGGTGAT 
TCAGAACACA AGCCCTACAT CTGTGGCGAT GCTGACTGCA GCACCTTCAA CCTGGACGGC 
AGCGAGGACT ACTTGATCCT GGCCTGCGAC GGCTTCTACG ACACAGTGAA CCCAGAAGAA 
GCCGTCCGAG TGGTGAGCGA CCACTTGCAG GAGAACAACG GGGACACCGC CATGGTGGCC 
CACAAGCTGG TGGCCTCAGC CCGAGATGCG GGGTCCAGTG ACAACATCAC GGTAATCGTA 
GTGTTCCTCA GAGACCCCCG TTTGCCTCCG CCCTCAGATG AGCCTGAGGA GCAGCAGGAT 
GAGGAGCCAT TGGAGGACGC AGGAGAAGAG GAAGAAGAGG AAGAAGCAGT GGATGCCTTG 
CAGAGTGAAT TGGTGTGTCA AGATGGAGGT GCGGAGAATG GAGGAAAGAA CCGGGGCGGC 
TGGCCTCTGC AGCAGTGCTC AGCGCCAGCT GACCTGGCCT ACGAAGACCG CATGGATTCA 
TTCACGGACA GAACAAGTCT GAGCATCACA GGGCCGGATG TGCATGCAGA GACTGGTTGC 
TTCAGGATCC CAGCTTCATC TGGCCTGCCC CCAACCAGAA CCGTCACGCC AGACCTCATT 
CCCTCCAACA GCAGGGCCGG CGCTGCCTGG CGTCCTTACC AAGAGGACGA CCCTTCTGGG 
TACAGGCAAA AACCCCAGCC TAATCCTAGA GTGCCCTCCT ACATGCAGAC CACCTCAGAT 
GGACAATTGC TGGAGGTGGC TGCGCTCTTC TCTCAGGAGG GCCGCAAGAA GAGGCGGCTG 
GACGTCCTGC CGCTGAGGAG GGAATCACAG CGCCTGACCC GGAGGGCCAG GGAAAGCCAC 
GGCCCTCTGA GCTGCTTCCC TAGTGTGCCC TATCCTCTGC GCTCCTCAGA GAGGAAGCCC 
GGGATAGCCT TTCCTCATGT AACCCATAGC AAAAGGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 07)
Emerson SE,St Clair RM,Waldron AL,Bruno SR,Duong A,Driscoll HE,Ballif BA,McFarlane S,Ebert AM

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phosphorylation and activation of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N/PPM1E) by Ca2+/calmodulin-dependent protein kinase I (CaMKI).
Biochemical and biophysical research communications422(4)703-9(2012 Jun)
Onouchi T,Sueyoshi N,Ishida A,Kameshita I

Functional processing of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N): evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, subcellular localization and substrate targeting in vivo.
Archives of biochemistry and biophysics517(1)43-52(2012 Jan)
Sueyoshi N,Nimura T,Onouchi T,Baba H,Takenaka S,Ishida A,Kameshita I

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ppm1e cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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