PPM1E cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPM1E
Entrez Gene ID	22843
Full Name	protein phosphatase, Mg2+/Mn2+ dependent 1E
Synonyms	CaMKP-N,POPX1,PP2CH,caMKN
General protein information	Preferred Names protein phosphatase, Mg2+/Mn2+ dependent 1E Names protein phosphatase 1E ca(2+)/calmodulin-dependent protein kinase phosphatase N caMKP-nucleus nuclear calmodulin-dependent protein kinase phosphatase partner of PIX 1 partner of PIX-alpha partner of PIXA protein phosphatase 1E (PP2C domain containing)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q22
Summary	This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017024359.1	XP_016879848.1	protein phosphatase 1E isoform X1
NM_014906.5	NP_055721.3	protein phosphatase 1E
XM_011524534.2	XP_011522836.1	protein phosphatase 1E isoform X1
XM_005257169.4	XP_005257226.1	protein phosphatase 1E isoform X1
XM_024450657.1	XP_024306425.1	protein phosphatase 1E isoform X1
NM_014906.4	NP_055721.3	protein phosphatase 1E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppm1e	NP_942068.1
Canis lupus familiaris (dog)	PPM1E	XP_853253.1
Gallus gallus (chicken)	PPM1E	XP_415871.4
Homo sapiens (human)	PPM1E	NP_055721.3
Danio rerio (zebrafish)	ppm1e	NP_001018354.1
Pan troglodytes (chimpanzee)	PPM1E	XP_523813.4
Macaca mulatta (Rhesus monkey)	LOC711012	XP_002800484.1
Bos taurus (cattle)	PPM1E	XP_002695648.3
Mus musculus (house mouse)	Ppm1e	NP_796141.2
Xenopus tropicalis (tropical clawed frog)	ppm1e	XP_002934392.1

Gene Ontology
Bibliography

Related articles in PubMed

AMPKα phosphatase Ppm1E upregulation in human gastric cancer is required for cell proliferation.
Chen MB, Liu YY, Cheng LB, Lu JW, Zeng P, Lu PH
Oncotarget8(19)31288-31296(2017 May)

microRNA-135b expression silences Ppm1e to provoke AMPK activation and inhibit osteoblastoma cell proliferation.
Li ZW, Zhu YR, Zhou XZ, Zhuo BB, Wang XD
Oncotarget8(16)26424-26433(2017 Apr)

miR-135b-5p inhibits LPS-induced TNFα production via silencing AMPK phosphatase Ppm1e.
Li P, Fan JB, Gao Y, Zhang M, Zhang L, Yang N, Zhao X
Oncotarget7(47)77978-77986(2016 Nov)

miR-135b expression downregulates Ppm1e to activate AMPK signaling and protect osteoblastic cells from dexamethasone.
Fan JB, Ruan JW, Liu W, Zhu LQ, Zhu XH, Yi H, Cui SY, Zhao JN, Cui ZM
Oncotarget7(43)70613-70622(2016 Oct)

POPX2 phosphatase regulates the KIF3 kinesin motor complex.
Phang HQ, Hoon JL, Lai SK, Zeng Y, Chiam KH, Li HY, Koh CG
Journal of cell science127(Pt 4)727-39(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPM1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPM1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08016	NM_014906.5 Latest version!	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu50227	XM_011524534.2 Latest version!	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu50227	XM_005257169.4 Latest version!	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu86694	XM_024450657.1 Latest version!	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu86694	XM_017024359.1 Latest version!	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu08016	NM_014906.4	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPM1E ( NM_014906.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055721.3 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant 1, mRNA.

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CloneID	OHu08016
Clone ID Related Accession (Same CDS sequence)	NM_014906.5 , NM_014906.4
Accession Version	NM_014906.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	protein phosphatase 1E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025521.21, AK289966.1 and AC100832.2. On Nov 22, 2018 this sequence version replaced NM_014906.4. Transcript Variant: This variant (1) represents the longer transcript and is protein-coding. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.165465.1, BC151228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308249.4/ ENSP00000312411.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT ATTCCTGGGC 
GAGTTTCGCG GACCGTGCGG CGGCGGCGAG CCGGAGCCGG AACCCGAACC CGAACCCGAA 
CCCGAACCCG AGTCCGAGCC CGAGCCCGAA CCTGAACTGG TAGAAGCTGA GGCGGCCGAG 
GCTTCGGTAG AGGAACCCGG GGAGGAGGCG GCCACGGTAG CCGCGACGGA GGAGGGGGAC 
CAGGAGCAAG ACCCGGAGCC CGAGGAGGAG GCGGCGGTTG AGGGTGAGGA GGAGGAGGAG 
GGCGCGGCGA CGGCGGCGGC AGCCCCGGGG CACTCGGCCG TGCCGCCGCC GCCGCCCCAG 
CTGCCGCCTT TGCCCCCGCT CCCGCGACCG CTGTCAGAGC GCATCACCCG CGAGGAGGTG 
GAGGGCGAAA GCCTGGACCT GTGCCTGCAG CAGCTCTACA AATATAATTG CCCTTCCTTT 
TTGGCTGCTG CTTTAGCCAG AGCCACATCA GATGAAGTCC TTCAGAGTGA TCTTTCTGCA 
CATTATATCC CAAAGGAAAC GGATGGCACA GAAGGGACTG TGGAGATTGA GACAGTGAAA 
TTGGCCCGTT CTGTCTTCAG CAAACTACAC GAGATTTGCT GCAGCTGGGT GAAAGACTTC 
CCCCTCCGCA GGAGACCCCA GCTTTATTAT GAGACATCAA TCCATGCCAT CAAAAACATG 
CGCAGGAAAA TGGAGGACAA ACATGTCTGC ATTCCTGACT TTAATATGCT CTTCAACCTA 
GAGGACCAGG AAGAACAAGC TTACTTTGCA GTGTTTGATG GCCATGGGGG AGTAGATGCT 
GCTATTTATG CCTCCATTCA CCTCCACGTT AACTTAGTCC GCCAGGAGAT GTTCCCCCAT 
GATCCTGCTG AGGCCCTGTG CAGGGCCTTC CGGGTCACTG ATGAGCGGTT TGTGCAGAAA 
GCAGCCAGGG AGAGCTTAAG ATGTGGGACC ACAGGAGTGG TGACTTTCAT CAGAGGCAAC 
ATGCTACATG TGGCCTGGGT GGGTGATTCC CAGGTTATGC TTGTGAGAAA GGGCCAAGCT 
GTTGAACTAA TGAAGCCACA CAAACCAGAC AGAGAGGATG AAAAGCAGAG AATTGAGGCC 
CTTGGAGGTT GCGTAGTCTG GTTTGGTGCC TGGAGGGTGA ATGGAAGTCT GTCGGTTTCC 
AGAGCTATTG GAGATGCTGA ACATAAGCCA TATATCTGTG GGGATGCAGA TTCTGCCTCC 
ACTGTTCTGG ATGGGACCGA AGACTACCTC ATTCTGGCCT GTGATGGCTT CTATGACACC 
GTGAACCCTG ATGAGGCAGT GAAAGTTGTG TCCGACCACC TGAAAGAGAA TAATGGAGAC 
AGCAGCATGG TTGCCCACAA ATTAGTGGCA TCAGCTCGTG ATGCTGGGTC AAGTGATAAC 
ATCACGGTTA TTGTGGTATT CCTGAGGGAC ATGAACAAAG CTGTAAATGT TAGTGAGGAA 
TCAGATTGGA CAGAGAACTC TTTTCAAGGA GGGCAAGAAG ATGGTGGGGA TGATAAGGAG 
AATCATGGAG AGTGCAAACG CCCTTGGCCT CAGCACCAGT GCTCAGCACC AGCCGACCTA 
GGCTATGATG GGCGTGTGGA TTCATTCACT GATAGAACTA GCCTGAGCCC AGGGTCCCAA 
ATCAACGTGC TGGAAGACCC AGGCTACCTA GATCTCACAC AAATAGAAGC AAGCAAACCT 
CACAGTGCCC AGTTTTTGCT ACCAGTTGAG ATGTTTGGTC CTGGTGCACC AAAGAAAGCA 
AATCTTATTA ATGAGTTAAT GATGGAGAAA AAATCAGTTC AGTCATCATT GCCTGAATGG 
AGTGGTGCTG GAGAGTTTCC CACTGCTTTC AATTTGGGTT CAACAGGGGA GCAGATATAC 
AGAATGCAGA GCTTGTCTCC TGTCTGTTCA GGGTTGGAAA ATGAACAGTT CAAATCCCCG 
GGAAACAGAG TTTCTAGATT GTCTCATTTA CGCCACCACT ACTCAAAGAA GTGGCACAGA 
TTCAGGTTTA ATCCAAAGTT TTATTCATTT CTCTCTGCTC AAGAGCCTTC CCACAAAATA 
GGCACTAGCC TGTCCTCACT TACTGGAAGT GGGAAGAGAA ATAGGATAAG AAGTTCTCTG 
CCATGGAGGC AAAATAGTTG GAAAGGGTAC AGTGAAAACA TGAGGAAGCT CAGAAAGACT 
CATGATATTC CATGCCCAGA TCTTCCTTGG AGCTATAAAA TAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055721.3
CDS	145..2412
Translation	MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPEPEPEPEPESEPEPEPELVEAEAAEASVEEPGEEAATVAATEEGDQEQDPEPEEEAAVEGEEEEEGAATAAAAPGHSAVPPPPPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVKLARSVFSKLHEICCSWVKDFPLRRRPQLYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE

Target ORF information:

RefSeq Version	NM_014906.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014906.5

ORF Insert Sequence:

ATGGCCGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT ATTCCTGGGC 
GAGTTTCGCG GACCGTGCGG CGGCGGCGAG CCGGAGCCGG AACCCGAACC CGAACCCGAA 
CCCGAACCCG AGTCCGAGCC CGAGCCCGAA CCTGAACTGG TAGAAGCTGA GGCGGCCGAG 
GCTTCGGTAG AGGAACCCGG GGAGGAGGCG GCCACGGTAG CCGCGACGGA GGAGGGGGAC 
CAGGAGCAAG ACCCGGAGCC CGAGGAGGAG GCGGCGGTTG AGGGTGAGGA GGAGGAGGAG 
GGCGCGGCGA CGGCGGCGGC AGCCCCGGGG CACTCGGCCG TGCCGCCGCC GCCGCCCCAG 
CTGCCGCCTT TGCCCCCGCT CCCGCGACCG CTGTCAGAGC GCATCACCCG CGAGGAGGTG 
GAGGGCGAAA GCCTGGACCT GTGCCTGCAG CAGCTCTACA AATATAATTG CCCTTCCTTT 
TTGGCTGCTG CTTTAGCCAG AGCCACATCA GATGAAGTCC TTCAGAGTGA TCTTTCTGCA 
CATTATATCC CAAAGGAAAC GGATGGCACA GAAGGGACTG TGGAGATTGA GACAGTGAAA 
TTGGCCCGTT CTGTCTTCAG CAAACTACAC GAGATTTGCT GCAGCTGGGT GAAAGACTTC 
CCCCTCCGCA GGAGACCCCA GCTTTATTAT GAGACATCAA TCCATGCCAT CAAAAACATG 
CGCAGGAAAA TGGAGGACAA ACATGTCTGC ATTCCTGACT TTAATATGCT CTTCAACCTA 
GAGGACCAGG AAGAACAAGC TTACTTTGCA GTGTTTGATG GCCATGGGGG AGTAGATGCT 
GCTATTTATG CCTCCATTCA CCTCCACGTT AACTTAGTCC GCCAGGAGAT GTTCCCCCAT 
GATCCTGCTG AGGCCCTGTG CAGGGCCTTC CGGGTCACTG ATGAGCGGTT TGTGCAGAAA 
GCAGCCAGGG AGAGCTTAAG ATGTGGGACC ACAGGAGTGG TGACTTTCAT CAGAGGCAAC 
ATGCTACATG TGGCCTGGGT GGGTGATTCC CAGGTTATGC TTGTGAGAAA GGGCCAAGCT 
GTTGAACTAA TGAAGCCACA CAAACCAGAC AGAGAGGATG AAAAGCAGAG AATTGAGGCC 
CTTGGAGGTT GCGTAGTCTG GTTTGGTGCC TGGAGGGTGA ATGGAAGTCT GTCGGTTTCC 
AGAGCTATTG GAGATGCTGA ACATAAGCCA TATATCTGTG GGGATGCAGA TTCTGCCTCC 
ACTGTTCTGG ATGGGACCGA AGACTACCTC ATTCTGGCCT GTGATGGCTT CTATGACACC 
GTGAACCCTG ATGAGGCAGT GAAAGTTGTG TCCGACCACC TGAAAGAGAA TAATGGAGAC 
AGCAGCATGG TTGCCCACAA ATTAGTGGCA TCAGCTCGTG ATGCTGGGTC AAGTGATAAC 
ATCACGGTTA TTGTGGTATT CCTGAGGGAC ATGAACAAAG CTGTAAATGT TAGTGAGGAA 
TCAGATTGGA CAGAGAACTC TTTTCAAGGA GGGCAAGAAG ATGGTGGGGA TGATAAGGAG 
AATCATGGAG AGTGCAAACG CCCTTGGCCT CAGCACCAGT GCTCAGCACC AGCCGACCTA 
GGCTATGATG GGCGTGTGGA TTCATTCACT GATAGAACTA GCCTGAGCCC AGGGTCCCAA 
ATCAACGTGC TGGAAGACCC AGGCTACCTA GATCTCACAC AAATAGAAGC AAGCAAACCT 
CACAGTGCCC AGTTTTTGCT ACCAGTTGAG ATGTTTGGTC CTGGTGCACC AAAGAAAGCA 
AATCTTATTA ATGAGTTAAT GATGGAGAAA AAATCAGTTC AGTCATCATT GCCTGAATGG 
AGTGGTGCTG GAGAGTTTCC CACTGCTTTC AATTTGGGTT CAACAGGGGA GCAGATATAC 
AGAATGCAGA GCTTGTCTCC TGTCTGTTCA GGGTTGGAAA ATGAACAGTT CAAATCCCCG 
GGAAACAGAG TTTCTAGATT GTCTCATTTA CGCCACCACT ACTCAAAGAA GTGGCACAGA 
TTCAGGTTTA ATCCAAAGTT TTATTCATTT CTCTCTGCTC AAGAGCCTTC CCACAAAATA 
GGCACTAGCC TGTCCTCACT TACTGGAAGT GGGAAGAGAA ATAGGATAAG AAGTTCTCTG 
CCATGGAGGC AAAATAGTTG GAAAGGGTAC AGTGAAAACA TGAGGAAGCT CAGAAAGACT 
CATGATATTC CATGCCCAGA TCTTCCTTGG AGCTATAAAA TAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPM1E ( NM_014906.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055721.3 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08016
Clone ID Related Accession (Same CDS sequence)	NM_014906.5 , NM_014906.4
Accession Version	NM_014906.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	protein phosphatase 1E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289966.1 and AC100832.2. On May 11, 2012 this sequence version replaced NM_014906.3. Transcript Variant: This variant (1) represents the longer transcript and is protein-coding. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK289966.1, SRR1660803.165465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT ATTCCTGGGC 
GAGTTTCGCG GACCGTGCGG CGGCGGCGAG CCGGAGCCGG AACCCGAACC CGAACCCGAA 
CCCGAACCCG AGTCCGAGCC CGAGCCCGAA CCTGAACTGG TAGAAGCTGA GGCGGCCGAG 
GCTTCGGTAG AGGAACCCGG GGAGGAGGCG GCCACGGTAG CCGCGACGGA GGAGGGGGAC 
CAGGAGCAAG ACCCGGAGCC CGAGGAGGAG GCGGCGGTTG AGGGTGAGGA GGAGGAGGAG 
GGCGCGGCGA CGGCGGCGGC AGCCCCGGGG CACTCGGCCG TGCCGCCGCC GCCGCCCCAG 
CTGCCGCCTT TGCCCCCGCT CCCGCGACCG CTGTCAGAGC GCATCACCCG CGAGGAGGTG 
GAGGGCGAAA GCCTGGACCT GTGCCTGCAG CAGCTCTACA AATATAATTG CCCTTCCTTT 
TTGGCTGCTG CTTTAGCCAG AGCCACATCA GATGAAGTCC TTCAGAGTGA TCTTTCTGCA 
CATTATATCC CAAAGGAAAC GGATGGCACA GAAGGGACTG TGGAGATTGA GACAGTGAAA 
TTGGCCCGTT CTGTCTTCAG CAAACTACAC GAGATTTGCT GCAGCTGGGT GAAAGACTTC 
CCCCTCCGCA GGAGACCCCA GCTTTATTAT GAGACATCAA TCCATGCCAT CAAAAACATG 
CGCAGGAAAA TGGAGGACAA ACATGTCTGC ATTCCTGACT TTAATATGCT CTTCAACCTA 
GAGGACCAGG AAGAACAAGC TTACTTTGCA GTGTTTGATG GCCATGGGGG AGTAGATGCT 
GCTATTTATG CCTCCATTCA CCTCCACGTT AACTTAGTCC GCCAGGAGAT GTTCCCCCAT 
GATCCTGCTG AGGCCCTGTG CAGGGCCTTC CGGGTCACTG ATGAGCGGTT TGTGCAGAAA 
GCAGCCAGGG AGAGCTTAAG ATGTGGGACC ACAGGAGTGG TGACTTTCAT CAGAGGCAAC 
ATGCTACATG TGGCCTGGGT GGGTGATTCC CAGGTTATGC TTGTGAGAAA GGGCCAAGCT 
GTTGAACTAA TGAAGCCACA CAAACCAGAC AGAGAGGATG AAAAGCAGAG AATTGAGGCC 
CTTGGAGGTT GCGTAGTCTG GTTTGGTGCC TGGAGGGTGA ATGGAAGTCT GTCGGTTTCC 
AGAGCTATTG GAGATGCTGA ACATAAGCCA TATATCTGTG GGGATGCAGA TTCTGCCTCC 
ACTGTTCTGG ATGGGACCGA AGACTACCTC ATTCTGGCCT GTGATGGCTT CTATGACACC 
GTGAACCCTG ATGAGGCAGT GAAAGTTGTG TCCGACCACC TGAAAGAGAA TAATGGAGAC 
AGCAGCATGG TTGCCCACAA ATTAGTGGCA TCAGCTCGTG ATGCTGGGTC AAGTGATAAC 
ATCACGGTTA TTGTGGTATT CCTGAGGGAC ATGAACAAAG CTGTAAATGT TAGTGAGGAA 
TCAGATTGGA CAGAGAACTC TTTTCAAGGA GGGCAAGAAG ATGGTGGGGA TGATAAGGAG 
AATCATGGAG AGTGCAAACG CCCTTGGCCT CAGCACCAGT GCTCAGCACC AGCCGACCTA 
GGCTATGATG GGCGTGTGGA TTCATTCACT GATAGAACTA GCCTGAGCCC AGGGTCCCAA 
ATCAACGTGC TGGAAGACCC AGGCTACCTA GATCTCACAC AAATAGAAGC AAGCAAACCT 
CACAGTGCCC AGTTTTTGCT ACCAGTTGAG ATGTTTGGTC CTGGTGCACC AAAGAAAGCA 
AATCTTATTA ATGAGTTAAT GATGGAGAAA AAATCAGTTC AGTCATCATT GCCTGAATGG 
AGTGGTGCTG GAGAGTTTCC CACTGCTTTC AATTTGGGTT CAACAGGGGA GCAGATATAC 
AGAATGCAGA GCTTGTCTCC TGTCTGTTCA GGGTTGGAAA ATGAACAGTT CAAATCCCCG 
GGAAACAGAG TTTCTAGATT GTCTCATTTA CGCCACCACT ACTCAAAGAA GTGGCACAGA 
TTCAGGTTTA ATCCAAAGTT TTATTCATTT CTCTCTGCTC AAGAGCCTTC CCACAAAATA 
GGCACTAGCC TGTCCTCACT TACTGGAAGT GGGAAGAGAA ATAGGATAAG AAGTTCTCTG 
CCATGGAGGC AAAATAGTTG GAAAGGGTAC AGTGAAAACA TGAGGAAGCT CAGAAAGACT 
CATGATATTC CATGCCCAGA TCTTCCTTGG AGCTATAAAA TAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055721.3
CDS	130..2397
Translation	MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPEPEPEPEPESEPEPEPELVEAEAAEASVEEPGEEAATVAATEEGDQEQDPEPEEEAAVEGEEEEEGAATAAAAPGHSAVPPPPPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVKLARSVFSKLHEICCSWVKDFPLRRRPQLYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE

Target ORF information:

RefSeq Version	NM_014906.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014906.4

ORF Insert Sequence:

ATGGCCGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT ATTCCTGGGC 
GAGTTTCGCG GACCGTGCGG CGGCGGCGAG CCGGAGCCGG AACCCGAACC CGAACCCGAA 
CCCGAACCCG AGTCCGAGCC CGAGCCCGAA CCTGAACTGG TAGAAGCTGA GGCGGCCGAG 
GCTTCGGTAG AGGAACCCGG GGAGGAGGCG GCCACGGTAG CCGCGACGGA GGAGGGGGAC 
CAGGAGCAAG ACCCGGAGCC CGAGGAGGAG GCGGCGGTTG AGGGTGAGGA GGAGGAGGAG 
GGCGCGGCGA CGGCGGCGGC AGCCCCGGGG CACTCGGCCG TGCCGCCGCC GCCGCCCCAG 
CTGCCGCCTT TGCCCCCGCT CCCGCGACCG CTGTCAGAGC GCATCACCCG CGAGGAGGTG 
GAGGGCGAAA GCCTGGACCT GTGCCTGCAG CAGCTCTACA AATATAATTG CCCTTCCTTT 
TTGGCTGCTG CTTTAGCCAG AGCCACATCA GATGAAGTCC TTCAGAGTGA TCTTTCTGCA 
CATTATATCC CAAAGGAAAC GGATGGCACA GAAGGGACTG TGGAGATTGA GACAGTGAAA 
TTGGCCCGTT CTGTCTTCAG CAAACTACAC GAGATTTGCT GCAGCTGGGT GAAAGACTTC 
CCCCTCCGCA GGAGACCCCA GCTTTATTAT GAGACATCAA TCCATGCCAT CAAAAACATG 
CGCAGGAAAA TGGAGGACAA ACATGTCTGC ATTCCTGACT TTAATATGCT CTTCAACCTA 
GAGGACCAGG AAGAACAAGC TTACTTTGCA GTGTTTGATG GCCATGGGGG AGTAGATGCT 
GCTATTTATG CCTCCATTCA CCTCCACGTT AACTTAGTCC GCCAGGAGAT GTTCCCCCAT 
GATCCTGCTG AGGCCCTGTG CAGGGCCTTC CGGGTCACTG ATGAGCGGTT TGTGCAGAAA 
GCAGCCAGGG AGAGCTTAAG ATGTGGGACC ACAGGAGTGG TGACTTTCAT CAGAGGCAAC 
ATGCTACATG TGGCCTGGGT GGGTGATTCC CAGGTTATGC TTGTGAGAAA GGGCCAAGCT 
GTTGAACTAA TGAAGCCACA CAAACCAGAC AGAGAGGATG AAAAGCAGAG AATTGAGGCC 
CTTGGAGGTT GCGTAGTCTG GTTTGGTGCC TGGAGGGTGA ATGGAAGTCT GTCGGTTTCC 
AGAGCTATTG GAGATGCTGA ACATAAGCCA TATATCTGTG GGGATGCAGA TTCTGCCTCC 
ACTGTTCTGG ATGGGACCGA AGACTACCTC ATTCTGGCCT GTGATGGCTT CTATGACACC 
GTGAACCCTG ATGAGGCAGT GAAAGTTGTG TCCGACCACC TGAAAGAGAA TAATGGAGAC 
AGCAGCATGG TTGCCCACAA ATTAGTGGCA TCAGCTCGTG ATGCTGGGTC AAGTGATAAC 
ATCACGGTTA TTGTGGTATT CCTGAGGGAC ATGAACAAAG CTGTAAATGT TAGTGAGGAA 
TCAGATTGGA CAGAGAACTC TTTTCAAGGA GGGCAAGAAG ATGGTGGGGA TGATAAGGAG 
AATCATGGAG AGTGCAAACG CCCTTGGCCT CAGCACCAGT GCTCAGCACC AGCCGACCTA 
GGCTATGATG GGCGTGTGGA TTCATTCACT GATAGAACTA GCCTGAGCCC AGGGTCCCAA 
ATCAACGTGC TGGAAGACCC AGGCTACCTA GATCTCACAC AAATAGAAGC AAGCAAACCT 
CACAGTGCCC AGTTTTTGCT ACCAGTTGAG ATGTTTGGTC CTGGTGCACC AAAGAAAGCA 
AATCTTATTA ATGAGTTAAT GATGGAGAAA AAATCAGTTC AGTCATCATT GCCTGAATGG 
AGTGGTGCTG GAGAGTTTCC CACTGCTTTC AATTTGGGTT CAACAGGGGA GCAGATATAC 
AGAATGCAGA GCTTGTCTCC TGTCTGTTCA GGGTTGGAAA ATGAACAGTT CAAATCCCCG 
GGAAACAGAG TTTCTAGATT GTCTCATTTA CGCCACCACT ACTCAAAGAA GTGGCACAGA 
TTCAGGTTTA ATCCAAAGTT TTATTCATTT CTCTCTGCTC AAGAGCCTTC CCACAAAATA 
GGCACTAGCC TGTCCTCACT TACTGGAAGT GGGAAGAGAA ATAGGATAAG AAGTTCTCTG 
CCATGGAGGC AAAATAGTTG GAAAGGGTAC AGTGAAAACA TGAGGAAGCT CAGAAAGACT 
CATGATATTC CATGCCCAGA TCTTCCTTGG AGCTATAAAA TAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPM1E ( XM_011524534.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522836.1 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50227
Clone ID Related Accession (Same CDS sequence)	XM_011524534.2 , XM_005257169.4
Accession Version	XM_011524534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	protein phosphatase 1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCT TCAGAGTGAT CTTTCTGCAC ATTATATCCC AAAGGAAACG GATGGCACAG 
AAGGGACTGT GGGACCAGGA AGAACAAGCT TACTTTGCAG TGTTTGATGG CCATGGGGGA 
GTAGATGCTG CTATTTATGC CTCCATTCAC CTCCACGTTA ACTTAGTCCG CCAGGAGATG 
TTCCCCCATG ATCCTGCTGA GGCCCTGTGC AGGGCCTTCC GGGTCACTGA TGAGCGGTTT 
GTGCAGAAAG CAGCCAGGGA GAGCTTAAGA TGTGGGACCA CAGGAGTGGT GACTTTCATC 
AGAGGCAACA TGCTACATGT GGCCTGGGTG GGTGATTCCC AGGTTATGCT TGTGAGAAAG 
GGCCAAGCTG TTGAACTAAT GAAGCCACAC AAACCAGACA GAGAGGATGA AAAGCAGAGA 
ATTGAGGCCC TTGGAGGTTG CGTAGTCTGG TTTGGTGCCT GGAGGGTGAA TGGAAGTCTG 
TCGGTTTCCA GAGCTATTGG AGATGCTGAA CATAAGCCAT ATATCTGTGG GGATGCAGAT 
TCTGCCTCCA CTGTTCTGGA TGGGACCGAA GACTACCTCA TTCTGGCCTG TGATGGCTTC 
TATGACACCG TGAACCCTGA TGAGGCAGTG AAAGTTGTGT CCGACCACCT GAAAGAGAAT 
AATGGAGACA GCAGCATGGT TGCCCACAAA TTAGTGGCAT CAGCTCGTGA TGCTGGGTCA 
AGTGATAACA TCACGGTTAT TGTGGTATTC CTGAGGGACA TGAACAAAGC TGTAAATGTT 
AGTGAGGAAT CAGATTGGAC AGAGAACTCT TTTCAAGGAG GGCAAGAAGA TGGTGGGGAT 
GATAAGGAGA ATCATGGAGA GTGCAAACGC CCTTGGCCTC AGCACCAGTG CTCAGCACCA 
GCCGACCTAG GCTATGATGG GCGTGTGGAT TCATTCACTG ATAGAACTAG CCTGAGCCCA 
GGGTCCCAAA TCAACGTGCT GGAAGACCCA GGCTACCTAG ATCTCACACA AATAGAAGCA 
AGCAAACCTC ACAGTGCCCA GTTTTTGCTA CCAGTTGAGA TGTTTGGTCC TGGTGCACCA 
AAGAAAGCAA ATCTTATTAA TGAGTTAATG ATGGAGAAAA AATCAGTTCA GTCATCATTG 
CCTGAATGGA GTGGTGCTGG AGAGTTTCCC ACTGCTTTCA ATTTGGGTTC AACAGGGGAG 
CAGATATACA GAATGCAGAG CTTGTCTCCT GTCTGTTCAG GGTTGGAAAA TGAACAGTTC 
AAATCCCCGG GAAACAGAGT TTCTAGATTG TCTCATTTAC GCCACCACTA CTCAAAGAAG 
TGGCACAGAT TCAGGTTTAA TCCAAAGTTT TATTCATTTC TCTCTGCTCA AGAGCCTTCC 
CACAAAATAG GCACTAGCCT GTCCTCACTT ACTGGAAGTG GGAAGAGAAA TAGGATAAGA 
AGTTCTCTGC CATGGAGGCA AAATAGTTGG AAAGGGTACA GTGAAAACAT GAGGAAGCTC 
AGAAAGACTC ATGATATTCC ATGCCCAGAT CTTCCTTGGA GCTATAAAAT AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522836.1
CDS	962..2518
Translation	MKSFRVIFLHIISQRKRMAQKGLWDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE

Target ORF information:

RefSeq Version	XM_011524534.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524534.2

ORF Insert Sequence:

ATGAAGTCCT TCAGAGTGAT CTTTCTGCAC ATTATATCCC AAAGGAAACG GATGGCACAG 
AAGGGACTGT GGGACCAGGA AGAACAAGCT TACTTTGCAG TGTTTGATGG CCATGGGGGA 
GTAGATGCTG CTATTTATGC CTCCATTCAC CTCCACGTTA ACTTAGTCCG CCAGGAGATG 
TTCCCCCATG ATCCTGCTGA GGCCCTGTGC AGGGCCTTCC GGGTCACTGA TGAGCGGTTT 
GTGCAGAAAG CAGCCAGGGA GAGCTTAAGA TGTGGGACCA CAGGAGTGGT GACTTTCATC 
AGAGGCAACA TGCTACATGT GGCCTGGGTG GGTGATTCCC AGGTTATGCT TGTGAGAAAG 
GGCCAAGCTG TTGAACTAAT GAAGCCACAC AAACCAGACA GAGAGGATGA AAAGCAGAGA 
ATTGAGGCCC TTGGAGGTTG CGTAGTCTGG TTTGGTGCCT GGAGGGTGAA TGGAAGTCTG 
TCGGTTTCCA GAGCTATTGG AGATGCTGAA CATAAGCCAT ATATCTGTGG GGATGCAGAT 
TCTGCCTCCA CTGTTCTGGA TGGGACCGAA GACTACCTCA TTCTGGCCTG TGATGGCTTC 
TATGACACCG TGAACCCTGA TGAGGCAGTG AAAGTTGTGT CCGACCACCT GAAAGAGAAT 
AATGGAGACA GCAGCATGGT TGCCCACAAA TTAGTGGCAT CAGCTCGTGA TGCTGGGTCA 
AGTGATAACA TCACGGTTAT TGTGGTATTC CTGAGGGACA TGAACAAAGC TGTAAATGTT 
AGTGAGGAAT CAGATTGGAC AGAGAACTCT TTTCAAGGAG GGCAAGAAGA TGGTGGGGAT 
GATAAGGAGA ATCATGGAGA GTGCAAACGC CCTTGGCCTC AGCACCAGTG CTCAGCACCA 
GCCGACCTAG GCTATGATGG GCGTGTGGAT TCATTCACTG ATAGAACTAG CCTGAGCCCA 
GGGTCCCAAA TCAACGTGCT GGAAGACCCA GGCTACCTAG ATCTCACACA AATAGAAGCA 
AGCAAACCTC ACAGTGCCCA GTTTTTGCTA CCAGTTGAGA TGTTTGGTCC TGGTGCACCA 
AAGAAAGCAA ATCTTATTAA TGAGTTAATG ATGGAGAAAA AATCAGTTCA GTCATCATTG 
CCTGAATGGA GTGGTGCTGG AGAGTTTCCC ACTGCTTTCA ATTTGGGTTC AACAGGGGAG 
CAGATATACA GAATGCAGAG CTTGTCTCCT GTCTGTTCAG GGTTGGAAAA TGAACAGTTC 
AAATCCCCGG GAAACAGAGT TTCTAGATTG TCTCATTTAC GCCACCACTA CTCAAAGAAG 
TGGCACAGAT TCAGGTTTAA TCCAAAGTTT TATTCATTTC TCTCTGCTCA AGAGCCTTCC 
CACAAAATAG GCACTAGCCT GTCCTCACTT ACTGGAAGTG GGAAGAGAAA TAGGATAAGA 
AGTTCTCTGC CATGGAGGCA AAATAGTTGG AAAGGGTACA GTGAAAACAT GAGGAAGCTC 
AGAAAGACTC ATGATATTCC ATGCCCAGAT CTTCCTTGGA GCTATAAAAT AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPM1E ( XM_005257169.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257226.1 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu50227
Clone ID Related Accession (Same CDS sequence)	XM_011524534.2 , XM_005257169.4
Accession Version	XM_005257169.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	protein phosphatase 1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257169.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCT TCAGAGTGAT CTTTCTGCAC ATTATATCCC AAAGGAAACG GATGGCACAG 
AAGGGACTGT GGGACCAGGA AGAACAAGCT TACTTTGCAG TGTTTGATGG CCATGGGGGA 
GTAGATGCTG CTATTTATGC CTCCATTCAC CTCCACGTTA ACTTAGTCCG CCAGGAGATG 
TTCCCCCATG ATCCTGCTGA GGCCCTGTGC AGGGCCTTCC GGGTCACTGA TGAGCGGTTT 
GTGCAGAAAG CAGCCAGGGA GAGCTTAAGA TGTGGGACCA CAGGAGTGGT GACTTTCATC 
AGAGGCAACA TGCTACATGT GGCCTGGGTG GGTGATTCCC AGGTTATGCT TGTGAGAAAG 
GGCCAAGCTG TTGAACTAAT GAAGCCACAC AAACCAGACA GAGAGGATGA AAAGCAGAGA 
ATTGAGGCCC TTGGAGGTTG CGTAGTCTGG TTTGGTGCCT GGAGGGTGAA TGGAAGTCTG 
TCGGTTTCCA GAGCTATTGG AGATGCTGAA CATAAGCCAT ATATCTGTGG GGATGCAGAT 
TCTGCCTCCA CTGTTCTGGA TGGGACCGAA GACTACCTCA TTCTGGCCTG TGATGGCTTC 
TATGACACCG TGAACCCTGA TGAGGCAGTG AAAGTTGTGT CCGACCACCT GAAAGAGAAT 
AATGGAGACA GCAGCATGGT TGCCCACAAA TTAGTGGCAT CAGCTCGTGA TGCTGGGTCA 
AGTGATAACA TCACGGTTAT TGTGGTATTC CTGAGGGACA TGAACAAAGC TGTAAATGTT 
AGTGAGGAAT CAGATTGGAC AGAGAACTCT TTTCAAGGAG GGCAAGAAGA TGGTGGGGAT 
GATAAGGAGA ATCATGGAGA GTGCAAACGC CCTTGGCCTC AGCACCAGTG CTCAGCACCA 
GCCGACCTAG GCTATGATGG GCGTGTGGAT TCATTCACTG ATAGAACTAG CCTGAGCCCA 
GGGTCCCAAA TCAACGTGCT GGAAGACCCA GGCTACCTAG ATCTCACACA AATAGAAGCA 
AGCAAACCTC ACAGTGCCCA GTTTTTGCTA CCAGTTGAGA TGTTTGGTCC TGGTGCACCA 
AAGAAAGCAA ATCTTATTAA TGAGTTAATG ATGGAGAAAA AATCAGTTCA GTCATCATTG 
CCTGAATGGA GTGGTGCTGG AGAGTTTCCC ACTGCTTTCA ATTTGGGTTC AACAGGGGAG 
CAGATATACA GAATGCAGAG CTTGTCTCCT GTCTGTTCAG GGTTGGAAAA TGAACAGTTC 
AAATCCCCGG GAAACAGAGT TTCTAGATTG TCTCATTTAC GCCACCACTA CTCAAAGAAG 
TGGCACAGAT TCAGGTTTAA TCCAAAGTTT TATTCATTTC TCTCTGCTCA AGAGCCTTCC 
CACAAAATAG GCACTAGCCT GTCCTCACTT ACTGGAAGTG GGAAGAGAAA TAGGATAAGA 
AGTTCTCTGC CATGGAGGCA AAATAGTTGG AAAGGGTACA GTGAAAACAT GAGGAAGCTC 
AGAAAGACTC ATGATATTCC ATGCCCAGAT CTTCCTTGGA GCTATAAAAT AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257226.1
CDS	66..1622
Translation	MKSFRVIFLHIISQRKRMAQKGLWDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE

Target ORF information:

RefSeq Version	XM_005257169.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257169.4

ORF Insert Sequence:

ATGAAGTCCT TCAGAGTGAT CTTTCTGCAC ATTATATCCC AAAGGAAACG GATGGCACAG 
AAGGGACTGT GGGACCAGGA AGAACAAGCT TACTTTGCAG TGTTTGATGG CCATGGGGGA 
GTAGATGCTG CTATTTATGC CTCCATTCAC CTCCACGTTA ACTTAGTCCG CCAGGAGATG 
TTCCCCCATG ATCCTGCTGA GGCCCTGTGC AGGGCCTTCC GGGTCACTGA TGAGCGGTTT 
GTGCAGAAAG CAGCCAGGGA GAGCTTAAGA TGTGGGACCA CAGGAGTGGT GACTTTCATC 
AGAGGCAACA TGCTACATGT GGCCTGGGTG GGTGATTCCC AGGTTATGCT TGTGAGAAAG 
GGCCAAGCTG TTGAACTAAT GAAGCCACAC AAACCAGACA GAGAGGATGA AAAGCAGAGA 
ATTGAGGCCC TTGGAGGTTG CGTAGTCTGG TTTGGTGCCT GGAGGGTGAA TGGAAGTCTG 
TCGGTTTCCA GAGCTATTGG AGATGCTGAA CATAAGCCAT ATATCTGTGG GGATGCAGAT 
TCTGCCTCCA CTGTTCTGGA TGGGACCGAA GACTACCTCA TTCTGGCCTG TGATGGCTTC 
TATGACACCG TGAACCCTGA TGAGGCAGTG AAAGTTGTGT CCGACCACCT GAAAGAGAAT 
AATGGAGACA GCAGCATGGT TGCCCACAAA TTAGTGGCAT CAGCTCGTGA TGCTGGGTCA 
AGTGATAACA TCACGGTTAT TGTGGTATTC CTGAGGGACA TGAACAAAGC TGTAAATGTT 
AGTGAGGAAT CAGATTGGAC AGAGAACTCT TTTCAAGGAG GGCAAGAAGA TGGTGGGGAT 
GATAAGGAGA ATCATGGAGA GTGCAAACGC CCTTGGCCTC AGCACCAGTG CTCAGCACCA 
GCCGACCTAG GCTATGATGG GCGTGTGGAT TCATTCACTG ATAGAACTAG CCTGAGCCCA 
GGGTCCCAAA TCAACGTGCT GGAAGACCCA GGCTACCTAG ATCTCACACA AATAGAAGCA 
AGCAAACCTC ACAGTGCCCA GTTTTTGCTA CCAGTTGAGA TGTTTGGTCC TGGTGCACCA 
AAGAAAGCAA ATCTTATTAA TGAGTTAATG ATGGAGAAAA AATCAGTTCA GTCATCATTG 
CCTGAATGGA GTGGTGCTGG AGAGTTTCCC ACTGCTTTCA ATTTGGGTTC AACAGGGGAG 
CAGATATACA GAATGCAGAG CTTGTCTCCT GTCTGTTCAG GGTTGGAAAA TGAACAGTTC 
AAATCCCCGG GAAACAGAGT TTCTAGATTG TCTCATTTAC GCCACCACTA CTCAAAGAAG 
TGGCACAGAT TCAGGTTTAA TCCAAAGTTT TATTCATTTC TCTCTGCTCA AGAGCCTTCC 
CACAAAATAG GCACTAGCCT GTCCTCACTT ACTGGAAGTG GGAAGAGAAA TAGGATAAGA 
AGTTCTCTGC CATGGAGGCA AAATAGTTGG AAAGGGTACA GTGAAAACAT GAGGAAGCTC 
AGAAAGACTC ATGATATTCC ATGCCCAGAT CTTCCTTGGA GCTATAAAAT AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPM1E ( XM_017024359.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879848.1 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86694
Clone ID Related Accession (Same CDS sequence)	XM_017024359.1 , XM_024450657.1
Accession Version	XM_017024359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein phosphatase 1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGA AAATGGAGGA CAAACATGTC TGCATTCCTG ACTTTAATAT GCTCTTCAAC 
CTAGAGGACC AGGAAGAACA AGCTTACTTT GCAGTGTTTG ATGGCCATGG GGGAGTAGAT 
GCTGCTATTT ATGCCTCCAT TCACCTCCAC GTTAACTTAG TCCGCCAGGA GATGTTCCCC 
CATGATCCTG CTGAGGCCCT GTGCAGGGCC TTCCGGGTCA CTGATGAGCG GTTTGTGCAG 
AAAGCAGCCA GGGAGAGCTT AAGATGTGGG ACCACAGGAG TGGTGACTTT CATCAGAGGC 
AACATGCTAC ATGTGGCCTG GGTGGGTGAT TCCCAGGTTA TGCTTGTGAG AAAGGGCCAA 
GCTGTTGAAC TAATGAAGCC ACACAAACCA GACAGAGAGG ATGAAAAGCA GAGAATTGAG 
GCCCTTGGAG GTTGCGTAGT CTGGTTTGGT GCCTGGAGGG TGAATGGAAG TCTGTCGGTT 
TCCAGAGCTA TTGGAGATGC TGAACATAAG CCATATATCT GTGGGGATGC AGATTCTGCC 
TCCACTGTTC TGGATGGGAC CGAAGACTAC CTCATTCTGG CCTGTGATGG CTTCTATGAC 
ACCGTGAACC CTGATGAGGC AGTGAAAGTT GTGTCCGACC ACCTGAAAGA GAATAATGGA 
GACAGCAGCA TGGTTGCCCA CAAATTAGTG GCATCAGCTC GTGATGCTGG GTCAAGTGAT 
AACATCACGG TTATTGTGGT ATTCCTGAGG GACATGAACA AAGCTGTAAA TGTTAGTGAG 
GAATCAGATT GGACAGAGAA CTCTTTTCAA GGAGGGCAAG AAGATGGTGG GGATGATAAG 
GAGAATCATG GAGAGTGCAA ACGCCCTTGG CCTCAGCACC AGTGCTCAGC ACCAGCCGAC 
CTAGGCTATG ATGGGCGTGT GGATTCATTC ACTGATAGAA CTAGCCTGAG CCCAGGGTCC 
CAAATCAACG TGCTGGAAGA CCCAGGCTAC CTAGATCTCA CACAAATAGA AGCAAGCAAA 
CCTCACAGTG CCCAGTTTTT GCTACCAGTT GAGATGTTTG GTCCTGGTGC ACCAAAGAAA 
GCAAATCTTA TTAATGAGTT AATGATGGAG AAAAAATCAG TTCAGTCATC ATTGCCTGAA 
TGGAGTGGTG CTGGAGAGTT TCCCACTGCT TTCAATTTGG GTTCAACAGG GGAGCAGATA 
TACAGAATGC AGAGCTTGTC TCCTGTCTGT TCAGGGTTGG AAAATGAACA GTTCAAATCC 
CCGGGAAACA GAGTTTCTAG ATTGTCTCAT TTACGCCACC ACTACTCAAA GAAGTGGCAC 
AGATTCAGGT TTAATCCAAA GTTTTATTCA TTTCTCTCTG CTCAAGAGCC TTCCCACAAA 
ATAGGCACTA GCCTGTCCTC ACTTACTGGA AGTGGGAAGA GAAATAGGAT AAGAAGTTCT 
CTGCCATGGA GGCAAAATAG TTGGAAAGGG TACAGTGAAA ACATGAGGAA GCTCAGAAAG 
ACTCATGATA TTCCATGCCC AGATCTTCCT TGGAGCTATA AAATAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879848.1
CDS	1364..2914
Translation	MRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE

Target ORF information:

RefSeq Version	XM_017024359.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024359.1

ORF Insert Sequence:

ATGCGCAGGA AAATGGAGGA CAAACATGTC TGCATTCCTG ACTTTAATAT GCTCTTCAAC 
CTAGAGGACC AGGAAGAACA AGCTTACTTT GCAGTGTTTG ATGGCCATGG GGGAGTAGAT 
GCTGCTATTT ATGCCTCCAT TCACCTCCAC GTTAACTTAG TCCGCCAGGA GATGTTCCCC 
CATGATCCTG CTGAGGCCCT GTGCAGGGCC TTCCGGGTCA CTGATGAGCG GTTTGTGCAG 
AAAGCAGCCA GGGAGAGCTT AAGATGTGGG ACCACAGGAG TGGTGACTTT CATCAGAGGC 
AACATGCTAC ATGTGGCCTG GGTGGGTGAT TCCCAGGTTA TGCTTGTGAG AAAGGGCCAA 
GCTGTTGAAC TAATGAAGCC ACACAAACCA GACAGAGAGG ATGAAAAGCA GAGAATTGAG 
GCCCTTGGAG GTTGCGTAGT CTGGTTTGGT GCCTGGAGGG TGAATGGAAG TCTGTCGGTT 
TCCAGAGCTA TTGGAGATGC TGAACATAAG CCATATATCT GTGGGGATGC AGATTCTGCC 
TCCACTGTTC TGGATGGGAC CGAAGACTAC CTCATTCTGG CCTGTGATGG CTTCTATGAC 
ACCGTGAACC CTGATGAGGC AGTGAAAGTT GTGTCCGACC ACCTGAAAGA GAATAATGGA 
GACAGCAGCA TGGTTGCCCA CAAATTAGTG GCATCAGCTC GTGATGCTGG GTCAAGTGAT 
AACATCACGG TTATTGTGGT ATTCCTGAGG GACATGAACA AAGCTGTAAA TGTTAGTGAG 
GAATCAGATT GGACAGAGAA CTCTTTTCAA GGAGGGCAAG AAGATGGTGG GGATGATAAG 
GAGAATCATG GAGAGTGCAA ACGCCCTTGG CCTCAGCACC AGTGCTCAGC ACCAGCCGAC 
CTAGGCTATG ATGGGCGTGT GGATTCATTC ACTGATAGAA CTAGCCTGAG CCCAGGGTCC 
CAAATCAACG TGCTGGAAGA CCCAGGCTAC CTAGATCTCA CACAAATAGA AGCAAGCAAA 
CCTCACAGTG CCCAGTTTTT GCTACCAGTT GAGATGTTTG GTCCTGGTGC ACCAAAGAAA 
GCAAATCTTA TTAATGAGTT AATGATGGAG AAAAAATCAG TTCAGTCATC ATTGCCTGAA 
TGGAGTGGTG CTGGAGAGTT TCCCACTGCT TTCAATTTGG GTTCAACAGG GGAGCAGATA 
TACAGAATGC AGAGCTTGTC TCCTGTCTGT TCAGGGTTGG AAAATGAACA GTTCAAATCC 
CCGGGAAACA GAGTTTCTAG ATTGTCTCAT TTACGCCACC ACTACTCAAA GAAGTGGCAC 
AGATTCAGGT TTAATCCAAA GTTTTATTCA TTTCTCTCTG CTCAAGAGCC TTCCCACAAA 
ATAGGCACTA GCCTGTCCTC ACTTACTGGA AGTGGGAAGA GAAATAGGAT AAGAAGTTCT 
CTGCCATGGA GGCAAAATAG TTGGAAAGGG TACAGTGAAA ACATGAGGAA GCTCAGAAAG 
ACTCATGATA TTCCATGCCC AGATCTTCCT TGGAGCTATA AAATAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPM1E ( XM_024450657.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306425.1 Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X2, mRNA.

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CloneID	OHu86694
Clone ID Related Accession (Same CDS sequence)	XM_017024359.1 , XM_024450657.1
Accession Version	XM_024450657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein phosphatase 1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGA AAATGGAGGA CAAACATGTC TGCATTCCTG ACTTTAATAT GCTCTTCAAC 
CTAGAGGACC AGGAAGAACA AGCTTACTTT GCAGTGTTTG ATGGCCATGG GGGAGTAGAT 
GCTGCTATTT ATGCCTCCAT TCACCTCCAC GTTAACTTAG TCCGCCAGGA GATGTTCCCC 
CATGATCCTG CTGAGGCCCT GTGCAGGGCC TTCCGGGTCA CTGATGAGCG GTTTGTGCAG 
AAAGCAGCCA GGGAGAGCTT AAGATGTGGG ACCACAGGAG TGGTGACTTT CATCAGAGGC 
AACATGCTAC ATGTGGCCTG GGTGGGTGAT TCCCAGGTTA TGCTTGTGAG AAAGGGCCAA 
GCTGTTGAAC TAATGAAGCC ACACAAACCA GACAGAGAGG ATGAAAAGCA GAGAATTGAG 
GCCCTTGGAG GTTGCGTAGT CTGGTTTGGT GCCTGGAGGG TGAATGGAAG TCTGTCGGTT 
TCCAGAGCTA TTGGAGATGC TGAACATAAG CCATATATCT GTGGGGATGC AGATTCTGCC 
TCCACTGTTC TGGATGGGAC CGAAGACTAC CTCATTCTGG CCTGTGATGG CTTCTATGAC 
ACCGTGAACC CTGATGAGGC AGTGAAAGTT GTGTCCGACC ACCTGAAAGA GAATAATGGA 
GACAGCAGCA TGGTTGCCCA CAAATTAGTG GCATCAGCTC GTGATGCTGG GTCAAGTGAT 
AACATCACGG TTATTGTGGT ATTCCTGAGG GACATGAACA AAGCTGTAAA TGTTAGTGAG 
GAATCAGATT GGACAGAGAA CTCTTTTCAA GGAGGGCAAG AAGATGGTGG GGATGATAAG 
GAGAATCATG GAGAGTGCAA ACGCCCTTGG CCTCAGCACC AGTGCTCAGC ACCAGCCGAC 
CTAGGCTATG ATGGGCGTGT GGATTCATTC ACTGATAGAA CTAGCCTGAG CCCAGGGTCC 
CAAATCAACG TGCTGGAAGA CCCAGGCTAC CTAGATCTCA CACAAATAGA AGCAAGCAAA 
CCTCACAGTG CCCAGTTTTT GCTACCAGTT GAGATGTTTG GTCCTGGTGC ACCAAAGAAA 
GCAAATCTTA TTAATGAGTT AATGATGGAG AAAAAATCAG TTCAGTCATC ATTGCCTGAA 
TGGAGTGGTG CTGGAGAGTT TCCCACTGCT TTCAATTTGG GTTCAACAGG GGAGCAGATA 
TACAGAATGC AGAGCTTGTC TCCTGTCTGT TCAGGGTTGG AAAATGAACA GTTCAAATCC 
CCGGGAAACA GAGTTTCTAG ATTGTCTCAT TTACGCCACC ACTACTCAAA GAAGTGGCAC 
AGATTCAGGT TTAATCCAAA GTTTTATTCA TTTCTCTCTG CTCAAGAGCC TTCCCACAAA 
ATAGGCACTA GCCTGTCCTC ACTTACTGGA AGTGGGAAGA GAAATAGGAT AAGAAGTTCT 
CTGCCATGGA GGCAAAATAG TTGGAAAGGG TACAGTGAAA ACATGAGGAA GCTCAGAAAG 
ACTCATGATA TTCCATGCCC AGATCTTCCT TGGAGCTATA AAATAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306425.1
CDS	1166..2716
Translation	MRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE

Target ORF information:

RefSeq Version	XM_024450657.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1E (PPM1E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450657.1

ORF Insert Sequence:

ATGCGCAGGA AAATGGAGGA CAAACATGTC TGCATTCCTG ACTTTAATAT GCTCTTCAAC 
CTAGAGGACC AGGAAGAACA AGCTTACTTT GCAGTGTTTG ATGGCCATGG GGGAGTAGAT 
GCTGCTATTT ATGCCTCCAT TCACCTCCAC GTTAACTTAG TCCGCCAGGA GATGTTCCCC 
CATGATCCTG CTGAGGCCCT GTGCAGGGCC TTCCGGGTCA CTGATGAGCG GTTTGTGCAG 
AAAGCAGCCA GGGAGAGCTT AAGATGTGGG ACCACAGGAG TGGTGACTTT CATCAGAGGC 
AACATGCTAC ATGTGGCCTG GGTGGGTGAT TCCCAGGTTA TGCTTGTGAG AAAGGGCCAA 
GCTGTTGAAC TAATGAAGCC ACACAAACCA GACAGAGAGG ATGAAAAGCA GAGAATTGAG 
GCCCTTGGAG GTTGCGTAGT CTGGTTTGGT GCCTGGAGGG TGAATGGAAG TCTGTCGGTT 
TCCAGAGCTA TTGGAGATGC TGAACATAAG CCATATATCT GTGGGGATGC AGATTCTGCC 
TCCACTGTTC TGGATGGGAC CGAAGACTAC CTCATTCTGG CCTGTGATGG CTTCTATGAC 
ACCGTGAACC CTGATGAGGC AGTGAAAGTT GTGTCCGACC ACCTGAAAGA GAATAATGGA 
GACAGCAGCA TGGTTGCCCA CAAATTAGTG GCATCAGCTC GTGATGCTGG GTCAAGTGAT 
AACATCACGG TTATTGTGGT ATTCCTGAGG GACATGAACA AAGCTGTAAA TGTTAGTGAG 
GAATCAGATT GGACAGAGAA CTCTTTTCAA GGAGGGCAAG AAGATGGTGG GGATGATAAG 
GAGAATCATG GAGAGTGCAA ACGCCCTTGG CCTCAGCACC AGTGCTCAGC ACCAGCCGAC 
CTAGGCTATG ATGGGCGTGT GGATTCATTC ACTGATAGAA CTAGCCTGAG CCCAGGGTCC 
CAAATCAACG TGCTGGAAGA CCCAGGCTAC CTAGATCTCA CACAAATAGA AGCAAGCAAA 
CCTCACAGTG CCCAGTTTTT GCTACCAGTT GAGATGTTTG GTCCTGGTGC ACCAAAGAAA 
GCAAATCTTA TTAATGAGTT AATGATGGAG AAAAAATCAG TTCAGTCATC ATTGCCTGAA 
TGGAGTGGTG CTGGAGAGTT TCCCACTGCT TTCAATTTGG GTTCAACAGG GGAGCAGATA 
TACAGAATGC AGAGCTTGTC TCCTGTCTGT TCAGGGTTGG AAAATGAACA GTTCAAATCC 
CCGGGAAACA GAGTTTCTAG ATTGTCTCAT TTACGCCACC ACTACTCAAA GAAGTGGCAC 
AGATTCAGGT TTAATCCAAA GTTTTATTCA TTTCTCTCTG CTCAAGAGCC TTCCCACAAA 
ATAGGCACTA GCCTGTCCTC ACTTACTGGA AGTGGGAAGA GAAATAGGAT AAGAAGTTCT 
CTGCCATGGA GGCAAAATAG TTGGAAAGGG TACAGTGAAA ACATGAGGAA GCTCAGAAAG 
ACTCATGATA TTCCATGCCC AGATCTTCCT TGGAGCTATA AAATAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AMPKα phosphatase Ppm1E upregulation in human gastric cancer is required for cell proliferation.
Oncotarget8(19)31288-31296(2017 May)
Chen MB,Liu YY,Cheng LB,Lu JW,Zeng P,Lu PH

microRNA-135b expression silences Ppm1e to provoke AMPK activation and inhibit osteoblastoma cell proliferation.
Oncotarget8(16)26424-26433(2017 Apr)
Li ZW,Zhu YR,Zhou XZ,Zhuo BB,Wang XD

miR-135b-5p inhibits LPS-induced TNFα production via silencing AMPK phosphatase Ppm1e.
Oncotarget7(47)77978-77986(2016 Nov)
Li P,Fan JB,Gao Y,Zhang M,Zhang L,Yang N,Zhao X

miR-135b expression downregulates Ppm1e to activate AMPK signaling and protect osteoblastic cells from dexamethasone.
Oncotarget7(43)70613-70622(2016 Oct)
Fan JB,Ruan JW,Liu W,Zhu LQ,Zhu XH,Yi H,Cui SY,Zhao JN,Cui ZM

POPX2 phosphatase regulates the KIF3 kinesin motor complex.
Journal of cell science127(Pt 4)727-39(2014 Feb)
Phang HQ,Hoon JL,Lai SK,Zeng Y,Chiam KH,Li HY,Koh CG

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PPM1E cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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