mthfd1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mthfd1b
Entrez Gene ID	260441
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b
Synonyms	cb325,fc29f07,mthfd1,wu:fc29f07,zgc:55597
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b Names C-1-tetrahydrofolate synthase, cytoplasmic methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199529.1	NP_955823.1	C-1-tetrahydrofolate synthase, cytoplasmic

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTHFD1	NP_001076946.1
Danio rerio (zebrafish)	mthfd1b	NP_955823.1
Arabidopsis thaliana (thale cress)	THFS	NP_564571.1
Oryza sativa (rice)	Os09g0446800	NP_001063310.1
Pan troglodytes (chimpanzee)	MTHFD1	XP_510001.3
Gallus gallus (chicken)	MTHFD1	NP_001034392.1
Xenopus tropicalis (tropical clawed frog)	mthfd1	NP_001008007.1
Schizosaccharomyces pombe (fission yeast)	SPBC839.16	NP_595256.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002830	XP_312083.5
Neurospora crassa	NCU03877	XP_956550.1
Homo sapiens (human)	MTHFD1	NP_005947.3
Drosophila melanogaster (fruit fly)	pug	NP_731489.2
Caenorhabditis elegans (roundworm)	K07E3.4	NP_509360.1
Kluyveromyces lactis	KLLA0E16545g	XP_454695.1
Eremothecium gossypii	AGOS_ACL121C	NP_983283.2
Magnaporthe oryzae (rice blast fungus)	MGG_01014	XP_003717930.1
Macaca mulatta (Rhesus monkey)	MTHFD1	XP_001101708.2
Rattus norvegicus (Norway rat)	Mthfd1	NP_071953.1
Canis lupus familiaris (dog)	MTHFD1	XP_537476.2
Mus musculus (house mouse)	Mthfd1	NP_620084.2
Saccharomyces cerevisiae (baker's yeast)	ADE3	NP_011720.3

Gene Ontology
Bibliography

Related articles in PubMed

Genetic control of cellular morphogenesis in Müller glia.
Charlton-Perkins M, Almeida AD, MacDonald RB, Harris WA
Glia67(7)1401-1411(2019 07)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Ethanol-induced upregulation of 10-formyltetrahydrofolate dehydrogenase helps relieve ethanol-induced oxidative stress.
Hsiao TH, Lin CJ, Chung YS, Lee GH, Kao TT, Chang WN, Chen BH, Hung JJ, Fu TF
Molecular and cellular biology34(3)498-509(2014 Feb)

Disruption of the folate pathway in zebrafish causes developmental defects.
Lee MS, Bonner JR, Bernard DJ, Sanchez EL, Sause ET, Prentice RR, Burgess SM, Brody LC
BMC developmental biology1212(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mthfd1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mthfd1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00783	NM_199529.1 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b (mthfd1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mthfd1b ( NM_199529.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955823.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b (mthfd1b), mRNA.

Price & Availability↑

CloneID	ODa00783
Clone ID Related Accession (Same CDS sequence)	NM_199529.1
Accession Version	NM_199529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570809600000
Organism	Danio rerio(zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045396.1. On Jul 6, 2005 this sequence version replaced XM_679624.1. ##Evidence-Data-START## Transcript exon combination :: BC045396.1, BC075779.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGCTC AGATCATCTG CGGGAAAACC ATCTCAGCTC AGGTTCGAGA GAGGCTGAAG 
GAAGAGGTGG AGGAAATGAA AAAGACTCAT CCCAACTTCG AGCCAGGCCT TGTTGTGTTA 
CAGGTGGGAG ACAGAGATGA CTCAAACTTG TACATTAGCA TGAAGCTAAA GGCTGCTGCC 
CAGATTGGGA TCACTGCTGT CCATATAAAG CTTCCACAAA CTGCGACCGA GACAGAGGTT 
TTGCAGAGTG TGACTGAAGT CAACGAGAAC TCCAAGATCC ATGGGCTCAT AGTCCAGCTG 
CCCCTCGACT CCATCCACAC TATTAACACA GAAAGAGTCA TCAATTCTGT GGCTCCAGAG 
AAAGATGTGG ATGGTTTGAC TAGCATCAAT GCAGGGAAAC TGGCACGTGG TGATCTTGGA 
GACTGTTTTA TCCCATGTAC TCCTAATGGA TGTATGGAGC TCATCAAACA AACAGGTGTG 
TCTTTGGCAG GAAAGAGGGC TGTGGTTATC GGACGCAGTA AGATTGTTGG CGCACCAATG 
CATGATCTTC TCCTTTGGAA TCACGCAACT GTAACAACCT GTCATTCAAA GACTGCAGAC 
TTGGTCTCTG AGGTGGGAAA GGCAGACATC CTGGTGACTG GGATTGGCAA ACCAGAGATG 
GTGCATGGAG ATTGGCTGAA GACTGGTGCT GAGGTCATTG ACTGTGGAAT TAACACTATC 
GCAGATAGTA GCAGGCCAAG TGGGAAGAGA GTTGTTGGAG ACGTTCACTA TGACTCTGCC 
AAAGATCGAG CTGCGTTTAT CACTCCTGTG CCTGGAGGAG TTGGCCCAAT GACTGTGGCC 
ATGCTCATGA AGAACACAGT ATTGAGTGCC AAGCATTTCC TTCAAGCACA AAAACCTGGG 
AAGTGGACCA TAAATTACCC CACACTCAAC CTGCTGCGTC CTGTTCCCAG TGATGTGGCC 
ATCTCTCGCT CCTGTGTTCC TAAACCCATC GAGTGTCTTG CTAAGGAAAT TGGTCTTCTT 
TCGGATGAAG TTGAGCTCTA CGGCAGGACC AAGGCCAAAG TCCAACTAAA AACCATTGAT 
CGACTGCAGA CTCAGCTTGA TGGCAAATAT GTGGTTGTAA CAGGAATCAC TCCGACTCCT 
CTGGGTGAAG GAAAGAGCAC CACCACAATA GGGTTGGTTC AAGCTCTCGG AGCTCATCTC 
AAACTCAATG CATTTGCGTG TGTGCGGCAG CCATCACAAG GCCCAACGTT TGGCATCAAA 
GGTGGTGCTG CTGGCGGGGG CTATTCTCAA GTCGTTCCCA TGGAAGAGTT CAATCTTCAT 
CTGACCGGTG ACATTCATGC CATTACTGCA GCCAACAACC CTGTAGCTGC TGCAGTGGAT 
GCTCGTATGT TCCATGAAGC CACGCAGTCT GATAAGGCTC TCTTTAACCG CTTGGTACCA 
TTGGCTGAGG GTCAGAGGAA GTTTTCCCCG GTTCAGATCA ACAGACTTGA AAGGCTAGGC 
ATAAAGAAAA CAGACCCCAG CACATTAACA GAGGAGGAAA TCACGCGCTT CGTCAGGCCG 
GACATTGACC CCGAGTCTGT CACTTGGCAG CGAGTGCTGG ACACAAATGA TCGTTTTCTT 
AGGAAAATCA CGATTGGCCA GTCTCCTACT GAGAAGGGCT TCACCAGGAC TGCCCAGTTT 
GACATCACTG TTGCCAGTGA GATTATGGCA GTTTTGGCTC TCACTACCGG CCTGGAGGAC 
ATGAAACAGA GGCTAGCCAA AATGGTGGTG GCCACCAGTC GCAGCGGTCA ACCTGTCACC 
ACAGAGGACT TGGGAGTGTG TGGAGCTCTG ACAGTCCTCA TGCGCGATGC TATCAAGCCA 
AACCTCATGC AGACTCTGGA GGGAAACCCT GTGTTTGTTC ATGCTGGGCC TTTCGCCAAC 
ATTGCACATG GAAACTCCTC AATCTTGGCT GATAAAATTG CTTTGAAACT AGTTGGACCT 
CAGGGATTTG TGGTCACAGA AGCTGGGTTT GGAGCAGATA TTGGAATGGA GAAATTTTTC 
AACATCAAGT GTCGCTACTC TGGTCTCAAG CCTCATGCTG TGGTGTTGGT TGCTACAGTC 
CGAGCACTTA AGATGCATGG AGGTGGTCCA ACGGTTACTG CAGGCACCCC TCTGCCAAAA 
GAATATATTG AAGAGAACCT GACTCTGCTG GAAGCTGGAT GTGCTAACAT GAGGAAACAA 
GTGGAGAATG CTAAGCTTTT TGGAGTTCCA GTAGTGGTTG CTGTCAATGC TTTCAAGACA 
GACACACAGG CTGAGCTAAA TCTGATCTGC AAGATGGCCA AAGAGGCAGG AGCGTTTGAT 
GCCGTGCCCT GCTCTCACTG GGCTGATGGT GGCGCTGGTG CTGTAGATCT GGCTCGAGCT 
GTTCAAAGAG CTGCAGACCT GCCCAACAGC TTCCAATTTC TCTATGATGT TCAGTTACCA 
ATAGCAGACA AGATCAGAAT CATTGCGCAG AAGATCTATG GTGCAGATGA CATTGAACTT 
CTCCCAGAGG CCCAACAAAA AGTGGAGCGG TACACCAAAC AGGGTTTTGC GGATCTGCCC 
ATATGTATGG CCAAAACTCA TCTGTCTCTC TCACACGATG CTGAAAAGAA AGGCGTTCCT 
ACTGGATTCG TTCTTCCCAT TCGTGACATT CGTGCTAGTG TTGGGGCAGG ATTCCTCTAC 
CCGCTTGTTG GCACTATGCC GACTATTCCT GGTCTTCCTA CTCGTCCATG TTTCTATGAC 
ATTGATCTGG ATACTGAGAG TGGAGAGGTC ATTGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955823.1
CDS	64..2868
Translation	MPAQIICGKTISAQVRERLKEEVEEMKKTHPNFEPGLVVLQVGDRDDSNLYISMKLKAAAQIGITAVHIKLPQTATETEVLQSVTEVNENSKIHGLIVQLPLDSIHTINTERVINSVAPEKDVDGLTSINAGKLARGDLGDCFIPCTPNGCMELIKQTGVSLAGKRAVVIGRSKIVGAPMHDLLLWNHATVTTCHSKTADLVSEVGKADILVTGIGKPEMVHGDWLKTGAEVIDCGINTIADSSRPSGKRVVGDVHYDSAKDRAAFITPVPGGVGPMTVAMLMKNTVLSAKHFLQAQKPGKWTINYPTLNLLRPVPSDVAISRSCVPKPIECLAKEIGLLSDEVELYGRTKAKVQLKTIDRLQTQLDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLKLNAFACVRQPSQGPTFGIKGGAAGGGYSQVVPMEEFNLHLTGDIHAITAANNPVAAAVDARMFHEATQSDKALFNRLVPLAEGQRKFSPVQINRLERLGIKKTDPSTLTEEEITRFVRPDIDPESVTWQRVLDTNDRFLRKITIGQSPTEKGFTRTAQFDITVASEIMAVLALTTGLEDMKQRLAKMVVATSRSGQPVTTEDLGVCGALTVLMRDAIKPNLMQTLEGNPVFVHAGPFANIAHGNSSILADKIALKLVGPQGFVVTEAGFGADIGMEKFFNIKCRYSGLKPHAVVLVATVRALKMHGGGPTVTAGTPLPKEYIEENLTLLEAGCANMRKQVENAKLFGVPVVVAVNAFKTDTQAELNLICKMAKEAGAFDAVPCSHWADGGAGAVDLARAVQRAADLPNSFQFLYDVQLPIADKIRIIAQKIYGADDIELLPEAQQKVERYTKQGFADLPICMAKTHLSLSHDAEKKGVPTGFVLPIRDIRASVGAGFLYPLVGTMPTIPGLPTRPCFYDIDLDTESGEVIGLF

Target ORF information:

RefSeq Version	NM_199529.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b (mthfd1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199529.1

ORF Insert Sequence:

ATGCCTGCTC AGATCATCTG CGGGAAAACC ATCTCAGCTC AGGTTCGAGA GAGGCTGAAG 
GAAGAGGTGG AGGAAATGAA AAAGACTCAT CCCAACTTCG AGCCAGGCCT TGTTGTGTTA 
CAGGTGGGAG ACAGAGATGA CTCAAACTTG TACATTAGCA TGAAGCTAAA GGCTGCTGCC 
CAGATTGGGA TCACTGCTGT CCATATAAAG CTTCCACAAA CTGCGACCGA GACAGAGGTT 
TTGCAGAGTG TGACTGAAGT CAACGAGAAC TCCAAGATCC ATGGGCTCAT AGTCCAGCTG 
CCCCTCGACT CCATCCACAC TATTAACACA GAAAGAGTCA TCAATTCTGT GGCTCCAGAG 
AAAGATGTGG ATGGTTTGAC TAGCATCAAT GCAGGGAAAC TGGCACGTGG TGATCTTGGA 
GACTGTTTTA TCCCATGTAC TCCTAATGGA TGTATGGAGC TCATCAAACA AACAGGTGTG 
TCTTTGGCAG GAAAGAGGGC TGTGGTTATC GGACGCAGTA AGATTGTTGG CGCACCAATG 
CATGATCTTC TCCTTTGGAA TCACGCAACT GTAACAACCT GTCATTCAAA GACTGCAGAC 
TTGGTCTCTG AGGTGGGAAA GGCAGACATC CTGGTGACTG GGATTGGCAA ACCAGAGATG 
GTGCATGGAG ATTGGCTGAA GACTGGTGCT GAGGTCATTG ACTGTGGAAT TAACACTATC 
GCAGATAGTA GCAGGCCAAG TGGGAAGAGA GTTGTTGGAG ACGTTCACTA TGACTCTGCC 
AAAGATCGAG CTGCGTTTAT CACTCCTGTG CCTGGAGGAG TTGGCCCAAT GACTGTGGCC 
ATGCTCATGA AGAACACAGT ATTGAGTGCC AAGCATTTCC TTCAAGCACA AAAACCTGGG 
AAGTGGACCA TAAATTACCC CACACTCAAC CTGCTGCGTC CTGTTCCCAG TGATGTGGCC 
ATCTCTCGCT CCTGTGTTCC TAAACCCATC GAGTGTCTTG CTAAGGAAAT TGGTCTTCTT 
TCGGATGAAG TTGAGCTCTA CGGCAGGACC AAGGCCAAAG TCCAACTAAA AACCATTGAT 
CGACTGCAGA CTCAGCTTGA TGGCAAATAT GTGGTTGTAA CAGGAATCAC TCCGACTCCT 
CTGGGTGAAG GAAAGAGCAC CACCACAATA GGGTTGGTTC AAGCTCTCGG AGCTCATCTC 
AAACTCAATG CATTTGCGTG TGTGCGGCAG CCATCACAAG GCCCAACGTT TGGCATCAAA 
GGTGGTGCTG CTGGCGGGGG CTATTCTCAA GTCGTTCCCA TGGAAGAGTT CAATCTTCAT 
CTGACCGGTG ACATTCATGC CATTACTGCA GCCAACAACC CTGTAGCTGC TGCAGTGGAT 
GCTCGTATGT TCCATGAAGC CACGCAGTCT GATAAGGCTC TCTTTAACCG CTTGGTACCA 
TTGGCTGAGG GTCAGAGGAA GTTTTCCCCG GTTCAGATCA ACAGACTTGA AAGGCTAGGC 
ATAAAGAAAA CAGACCCCAG CACATTAACA GAGGAGGAAA TCACGCGCTT CGTCAGGCCG 
GACATTGACC CCGAGTCTGT CACTTGGCAG CGAGTGCTGG ACACAAATGA TCGTTTTCTT 
AGGAAAATCA CGATTGGCCA GTCTCCTACT GAGAAGGGCT TCACCAGGAC TGCCCAGTTT 
GACATCACTG TTGCCAGTGA GATTATGGCA GTTTTGGCTC TCACTACCGG CCTGGAGGAC 
ATGAAACAGA GGCTAGCCAA AATGGTGGTG GCCACCAGTC GCAGCGGTCA ACCTGTCACC 
ACAGAGGACT TGGGAGTGTG TGGAGCTCTG ACAGTCCTCA TGCGCGATGC TATCAAGCCA 
AACCTCATGC AGACTCTGGA GGGAAACCCT GTGTTTGTTC ATGCTGGGCC TTTCGCCAAC 
ATTGCACATG GAAACTCCTC AATCTTGGCT GATAAAATTG CTTTGAAACT AGTTGGACCT 
CAGGGATTTG TGGTCACAGA AGCTGGGTTT GGAGCAGATA TTGGAATGGA GAAATTTTTC 
AACATCAAGT GTCGCTACTC TGGTCTCAAG CCTCATGCTG TGGTGTTGGT TGCTACAGTC 
CGAGCACTTA AGATGCATGG AGGTGGTCCA ACGGTTACTG CAGGCACCCC TCTGCCAAAA 
GAATATATTG AAGAGAACCT GACTCTGCTG GAAGCTGGAT GTGCTAACAT GAGGAAACAA 
GTGGAGAATG CTAAGCTTTT TGGAGTTCCA GTAGTGGTTG CTGTCAATGC TTTCAAGACA 
GACACACAGG CTGAGCTAAA TCTGATCTGC AAGATGGCCA AAGAGGCAGG AGCGTTTGAT 
GCCGTGCCCT GCTCTCACTG GGCTGATGGT GGCGCTGGTG CTGTAGATCT GGCTCGAGCT 
GTTCAAAGAG CTGCAGACCT GCCCAACAGC TTCCAATTTC TCTATGATGT TCAGTTACCA 
ATAGCAGACA AGATCAGAAT CATTGCGCAG AAGATCTATG GTGCAGATGA CATTGAACTT 
CTCCCAGAGG CCCAACAAAA AGTGGAGCGG TACACCAAAC AGGGTTTTGC GGATCTGCCC 
ATATGTATGG CCAAAACTCA TCTGTCTCTC TCACACGATG CTGAAAAGAA AGGCGTTCCT 
ACTGGATTCG TTCTTCCCAT TCGTGACATT CGTGCTAGTG TTGGGGCAGG ATTCCTCTAC 
CCGCTTGTTG GCACTATGCC GACTATTCCT GGTCTTCCTA CTCGTCCATG TTTCTATGAC 
ATTGATCTGG ATACTGAGAG TGGAGAGGTC ATTGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic control of cellular morphogenesis in Müller glia.
Glia67(7)1401-1411(2019 07)
Charlton-Perkins M,Almeida AD,MacDonald RB,Harris WA

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Ethanol-induced upregulation of 10-formyltetrahydrofolate dehydrogenase helps relieve ethanol-induced oxidative stress.
Molecular and cellular biology34(3)498-509(2014 Feb)
Hsiao TH,Lin CJ,Chung YS,Lee GH,Kao TT,Chang WN,Chen BH,Hung JJ,Fu TF

Disruption of the folate pathway in zebrafish causes developmental defects.
BMC developmental biology1212(2012 Apr)
Lee MS,Bonner JR,Bernard DJ,Sanchez EL,Sause ET,Prentice RR,Burgess SM,Brody LC

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mthfd1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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